Cytometry Part A

Cover image for Vol. 87 Issue 12

Edited By: Attila Tárnok

Impact Factor: 2.928

ISI Journal Citation Reports © Ranking: 2014: 28/79 (BIOCHEMICAL RESEARCH METHODS); 109/184 (Cell Biology)

Online ISSN: 1552-4930

Associated Title(s): Cytometry Part B: Clinical Cytometry

Information on OMIPs

Optimized Multicolor Immunofluorescence Panel (OMIP) is a special peer-reviewed Cytometry Part A publication type that reports on newly designed and optimized multicolor panels for flow cytometry, fluorescence microscopy, image cytometry, and other polychromatic fluorescence-based methods. The first two OMIPs were published in the September 2010 issue of the Journal.

OMIPs are aimed: (1) to alleviate the development time for researchers in need of the same or highly similar panels, (2) to provide a starting point for the creation of novel OMIPs, and (3) to give the developers of the panels credit via citation or the publication.

An OMIP consists of two portions:
i. A two-page printed component includes basic information, such as the title, a summary table, a brief narrative, a reagent table, an example staining figure and a detailed comparison with similar OMIPs.
ii. An online component includes significant amount of technical details, such as panel development strategy, cross-references to related panels, exact staining protocol, instrument configuration and reagent information.

For more information on OMIPs, please refer to the following articles:

OMIPs—Orchestrating multiplexity in polychromatic science
Mario Roederer and Attila Tárnok
Cytometry, 77A: 811–812.

Publication of optimized multicolor immunofluorescence panels
Yolanda Mahnke, Pratip Chattopadhyay, and Mario Roederer
Cytometry, 77A: 814–818

OMIPs published to date:

OMIP-001: Quality and phenotype of Ag-responsive human T-cells.
Yolanda Mahnke and Mario Roederer
Cytometry, 77A: 819–820

OMIP-002: Phenotypic analysis of specific human CD8 T-cells using peptide-MHC class I multimers for any of four epitopes.
Pratip Chattopadhyay, Mario Roederer, and David Price
Cytometry, 77A: 821–822

OMIP-003: Phenotypic analysis of human memory B cells
Chungwen Wei, John Jung, and Iñaki Sanz
Cytometry, 79A: 894–896

OMIP-004: In-depth characterization of human T regulatory cells
Angélique Biancotto, Pradeep Dagur, J. Chris Fuchs, Marc Langweiler, and J. Philip McCoy Jr,
Cytometry, 81A: 15–16

OMIP-005: Quality and phenotype of antigen-responsive rhesus macaque T cells
Kathryn Foulds, Mitzi Donaldson, and Mario Roederer
Cytometry, 81A: 360–361

OMIP-006: Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry
David Murdoch, Janet Staats, and Kent Weinhold
Cytometry, 81A: 281–283.

OMIP-007: Phenotypic analysis of human natural killer cells
Michael Eller and Jeffrey Currier
Cytometry, 81A: 447–449

OMIP-008: Measurement of Th1 and Th2 cytokine polyfunctionality of human T cells
Cindy Zuleger and Mark Albertini
Cytometry, 81A: 450–452

OMIP-009: Characterization of antigen-specific human T-cells
Laurie Lamoreaux, Richard Koup, and Mario Roederer
Cytometry, 81A: 362–363

OMIP-010: A new 10-color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples.
Frank Preijers, Erik Huys, and Bijan Moshaver
Cytometry, 81A: 453–455

OMIP-011: Characterization of circulating endothelial cells (CECs) in peripheral blood
Raskit Lachmann, Paola Lanuti, and Sebastiano Miscia
Cytometry, 81A: 549–551

OMIP-012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice
Brian Long and Cheryl Stoddart
Cytometry, 81A: 646–648

OMIP-013: Differentiation of human T-cells.
Yolanda Mahnke, Margaret Beddall, and Mario Roederer
Cytometry, 81A: 935–936

OMIP-014: Validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining
Stephen De Rosa, Donald Carter, and M. Juliana McElrath
Cytometry, 81A: 1019–1021

OMIP-015: Human regulatory and activated T-cells without intracellular staining
Yolanda Mahnke, Margaret Beddall, and Mario Roederer
Cytometry, 83A: 179–181

OMIP-016: Characterization of antigen-responsive macaque and human T-cells
Sabrina Guenounou, Nathalie Bosquet, Claudia Dembek, Roger Le Grand, and Antonio Cosma
Cytometry, 83A: 182–184

OMIP-017: Human CD4+ helper T-cell subsets including follicular helper cells
Yolanda Mahnke, Margaret Beddall, and Mario Roederer
Cytometry, 83A: 439–440

OMIP-018: Chemokine receptor expression on human T helper cells

Tess Brodie, Elena Brenna, and Federica Sallusto
Cytometry, 83A: 530–532

Corrigendum for OMIP-018: Chemokine receptor expression on human T helper cells
Tess Brodie, Elena Brenna, and Federica Sallusto
This article corrects
OMIP-018: Chemokine receptor expression on human T helper cells
Cytometry, 83A: 530–532

OMIP-019: Quantification of human γδT-cells, iNKT-cells, and hematopoietic precursors
Yolanda Mahnke, Margaret Beddall, and Mario Roederer
Cytometry, 83A: 676–678

OMIP-020: Phenotypic characterization of human γδ T-cells by multicolor flow cytometry.
Kilian Wistuba-Hamprecht, Graham Pawelec, and Evelyna Derhovanessian
Cytometry, 85: 522–524

OMIP-021: Simultaneous quantification of human conventional and innate-like T-cell subsets
Nicholas Gherardin, David Ritchie, Dale Godfrey, and Paul Neeson
Cytometry, 85: 573-575

OMIP-022: comprehensive assessment of antigen-specific human t-cell functionality and memory
Andrew Graves, Marcelino Padilla, and David Hokey
Cytometry, 85: 576-579

OMIP-023: 10-color, 13 antibody panel for in-depth phenotyping of human peripheral blood leukocytes
József Bocsi, Susanne Melzer, Ingo Dähnert and Attila Tárnok
Cytometry, 85: 781-784

OMIP-024: Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples
Gemma Moncunill, Hannah Han, Carlota Dobaño, M. Juliana McElrath and Stephen C. De Rosa
Cytometry, 85: 995-998

OMIP-025: Evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining
Gemma Moncunill, Carlota Dobaño, M. Juliana McElrath and Stephen C. De Rosa
Cytometry, 87: 289-292

OMIP-026: Phenotypic analysis of B and plasma cells in rhesus macaques
Berit Neumann, Sieghart Sopper and Christiane Stahl-Hennig
Cytometry, 87: 800-802

OMIP-027: Functional analysis of human natural killer cells
Margaret C. Costanzo, Matthew Creegan, Kerri G. Lal and Michael A. Eller
Cytometry, 87: 803-805

OMIP-028: Activation panel for Rhesus macaque NK cell subsets
Nicholas Pomplun, Kim L. Weisgrau, David T. Evans and Eva G. Rakasz
Cytometry, 87: 890–893

OMIP-029: Human NK-cell phenotypization
Yolanda D. Mahnke, Margaret H. Beddall and Mario Roederer
Cytometry, 87: 890–893