Sortase A-mediated site-specific labeling of camelid single-domain antibody-fragments: a versatile strategy for multiple molecular imaging modalities
Sam Massa, Niravkumar Vikani, Cecilia Betti, Steven Ballet, Saskia Vanderhaegen, Jan Steyaert, Benedicte Descamps, Christian Vanhove, Anton Bunschoten, Fijs W. B. van Leeuwen, Sophie Hernot, Vicky Caveliers, Tony Lahoutte, Serge Muyldermans, Catarina Xavier and Nick Devoogdt
Version of Record online: 5 MAY 2016 | DOI: 10.1002/cmmi.1696
Camelid single-domain antibody-fragments were labeled chemoenzymatically by Sortase A resulting in homogeneous imaging tracers. The conjugation of different imaging probes allowed tumor visualization in multiple imaging modalities, with high contrast as soon as 1 h post-injection.