Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Editor-in-Chief: José Oliveira; Deputy Editor: Mary Farrell
Online ISSN: 1613-6829
Associated Title(s): Advanced Energy Materials, Advanced Engineering Materials, Advanced Functional Materials, Advanced Healthcare Materials, Advanced Materials, Advanced Materials Interfaces, Advanced Optical Materials, Particle & Particle Systems Characterization
Single-Particle Tracking: Single-Particle Tracking of Hepatitis B Virus-like Vesicle Entry into Cells (Small 9/2011)
The cover image shows the visualization of the entry of a Hepatitis B virus-like vesicle (HBsAg) into a cell. HBsAg (shown as red particles) is used as a model to study the infection mechanisms and dynamics of the single-enveloped virus in living cells by real-time fluorescence microscopy. HBsAg is found to enter cells via a caveolin-mediated endocytic pathway (caveolin: yellow). By tracking individual HBsAg particles in living cells, the anomalously actin-dependent but not microtubule-dependent motility of internalized HBsAg particles is revealed (actin: green). The motility of HBsAg particles in living cells was also analyzed quantitatively. The results may potentially settle the long-term debate of whether hepatitis B viruses directly break the plasma membrane barrier or rely on endocytosis to infect the cell. For more information, please read the Full Paper “Single-Particle Tracking of Hepatitis B Virus-like Vesicle Entry into Cells” by Z. Huang, Z. Tang, H. Wang, and co-workers, beginning on page 1212.