8_06i/2012Imaging: Nanoscale Localization Sampling Based on Nanoantenna Arrays for Super-resolution Imaging of Fluorescent Monomers on Sliding Microtubules (Small 6/2012)

The cover image illustrates nanoscale localization sampling for super-resolution microscopy of microtubules sliding on nanoantenna arrays. Periodic nanohole arrays create local hot spots through surface plasmon localization. Subdiffraction-limited images are produced using the fluorescence excited by hot spots that sample microtubules labeled with rhodamine. A fourfold improvement is demonstrated in the spatial resolution below the diffraction limit at 76 nm resolution in the direction of movement and 135 nm orthogonally. Correlation studies between neighboring nanoantennas confirm the possibility of providing microtubular transport dynamics. The nanoantenna-based sampling scheme can be useful for moving objects that have a high labeling density or for performing fluctuation spectroscopy in small volumes. By customizing nanoantenna structures for specific resolution needs, scanless super-resolution microscopy may be possible on demand in the far-field. For more information, please read the Full Paper “Nanoscale Localization Sampling Based on Nanoantenna Arrays for Super-resolution Imaging of Fluorescent Monomers on Sliding Microtubules” by T. Nishizaka, D. Kim, and co-workers, beginning on page 892.

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