Journal of Separation Science
© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Impact Factor: 2.737
ISI Journal Citation Reports © Ranking: 2014: 21/74 (Chemistry Analytical)
Online ISSN: 1615-9314
Associated Title(s): ELECTROPHORESIS
July 31, 2007
Separation of three water-soluble vitamins by poly(dimethylsiloxane) microchannel electrophoresisA method for rapid separation and sensitive determination of three water-soluble vitamins, pyridoxine, ascorbic acid (VC) and para-aminobenzoic acid (PABA) has been developed by poly(dimethylsiloxane) (PDMS) microchannel electrophoresis integrated with amperometric detection. After the microchip was treated by oxygen plasma, peak shapes of three analytes were essentially improved. The proposed method has been successfully applied to actual human urine, without solid phase extraction (SPE) treatment, with the recoveries of 80%~122% for the three water-soluble vitamins.
The PDMS-microchip CE integrated with amperometric electrochemical detection has been demonstrated to be a new alternative for rapid separation and sensitive determination of pyridoxine, VC and PABA. The PDMS microchannels are modified by oxygen plasma, which resulted in short migration time, good peak shape and improved separation efficiency. This microsystem has been used to determine low levels of pyridoxine, VC and PABA in urine with satisfactory results, and no SPE procedure is necessary prior to analysis. Therefore, microchannel electrophoresis should have the potential applications for the determination of water-soluble vitamins in biological fluids.
J. Sep. Sci. 2007, 30 (14).
Full article online, DOI: 10.1002/jssc.200700155