Journal of Separation Science

Cover image for Vol. 39 Issue 8

Impact Factor: 2.737

ISI Journal Citation Reports © Ranking: 2014: 21/74 (Chemistry Analytical)

Online ISSN: 1615-9314

Associated Title(s): ELECTROPHORESIS

July 31, 2007

Determination of uric acid in plasma and allantoic fluid of chicken embryos by capillary electrophoresis

Uric acid is the end product of catabolism of purine nucleosides; adenosine and guanosine. In clinical practice, the uric acid tests are used to evaluate the elevated blood levels of uric acid to detect hypertension, vascular disease, Lesh-Nyham syndrome or gout, as well as to assess uric acid levels in urine for kidney stone formation. The urine test is most often used to monitor patients already diagnosed with kidney stones, but it can also serve to detect the metabolic disorders that affect the body's production of uric acid and to help measure the level of kidney functioning. Apart from the role of uric acid as a marker of series of diseases, it was shown that it exhibits anti-oxidant activity and protect living organisms from reactive free radicals.

Capillary electrophoresis (CE) with diode array detection (DAD) was used by Jana Matějčková, Petr Tůma, Eva Samcová, and Zdeňka Zemanová to determine uric acid in the chicken plasma and the allantoic fluid of the chicken embryos. Complete separation of uric and ascorbic acids was attained in less than 10. The volume of the plasma collected from a single chicken embryo amounts to approximately 10 μL. Such small volumes cannot be analyzed using standard enzymatic or chromatographic methods available in clinical laboratories. On the other hand, the volume of the sample injected into a CE capillary can be of order of units to tens of nL, and the CE analysis can be performed with the plasma samples as small as 1 μL. The limit of the detection was found to be low enough for sensitive analysis of native plasma and allantoic fluid samples. Also range of linearity, repeatability for peak area, and migration time, as well as recovery of uric acid from biological samples were found to be satisfactory. The method was applied to detect the elevated uric acid concentrations in the chicken embryos with induced unilateral renal agenesis.

Jana Matějčková, Petr Tůma, Eva Samcová, and Zdeňka Zemanová, J. Sep. Sci. 2007, 30 (12).
Full article online, DOI: 10.1002/jssc.20060046