© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Editor-in-Chief: Lorna Stimson, Deputy Editor: Lucie Kalvodova
Impact Factor: 4.079
ISI Journal Citation Reports © Ranking: 2015: 13/77 (BIOCHEMICAL RESEARCH METHODS); 75/289 (Biochemistry & Molecular Biology)
Online ISSN: 1615-9861
Associated Title(s): PROTEOMICS - Clinical Applications
Cover Picture: Proteomics 7/2008
In this issue of Proteomics you will find the following highlighted articles:
In thriller movies and spy stories, you can often tell which character is a bad guy if his “confession” changes under pressure or depends on the inquisitor. Likewise for peptides with modifications. Staes et al. use a similar technique to find α-amino blocked peptides. After chromatography of a digest over a C18 reverse phase column, fractions were treated with TNBS and re-chromatographed on the same column, under the same conditions. The peptides that had trypsin-exposed amino groups became much more hydrophobic in the second round because of the addition of the TNBS. The technique (COFRADIC) was also improved by preceding the C18 column by use of a strong cation exchange for fractionation and using a kit for removal of any pyrrolidone carboxylic acid termini from peptides. The revised protocol raised the yield of true amino termini from 60% to 95%.
Staes, A. et al., Proteomics 2008, 8, 1362–1370.
As hikers in North America and normal people in many parts of the world know, Cryptosporidium parvum is a protozoan parasite that causes an unpleasant intestinal infection in humans. It also infects livestock species, which leads to widespread waterborne transmission unless effective water treatment is employed. When the oocytes enter the gastrointestinal tract, they are stimulated to undergo excystation, releasing four sporozoites that enter the epithelial cells. There they undergo asexual reproduction and begin a complex series of steps before reproduction is complete and oocytes are released. Although the genome has been completely sequenced, many of the proteins predicted did not have recognizable functions. Sanderson et al. used a tissue culture system of excystation to collect enough sporozoites for proteomic analysis by MuDPIT and LC-MS/MS after (a) 2-DE and (b) 1-DE. Over 1200 unique proteins were identified, representing >30% of the predicted organism proteome, >200 of which had transmembrane domains.
Sanderson, S. J. et al., Proteomics 2008, 8, 1398–1414.
Reactive oxygen species (ROS) seem to be involved in a variety of diseases, including Alzheimer's, Parkinson's, cancer and heart disease. Searches for biomarkers for these diseases have most commonly been done in blood plasma, which contains proteins from essentially every cell type and tissue in the organism. Mirzaei et al. explore questions of cause and effect in rat plasma by trapping ROS-caused carbonylation points with biotin hydrazide, followed by avidin affinity chromatography and proteomic analysis (LC-MS/MS). Of 146 proteins identified in four rats, 44 had at least one carbonylation site and 38 had two or more sites. Over 30% of the proteins were membrane proteins, suggesting a major source of ROS was external, a hypothesis supported by the observation that mitochondrial proteins are not affected, despite their proximity to endogenous ROS. On the other hand, 13% were nuclear proteins. Another surprise: virtually no (2%) plasma proteins were found.
Mirzaei, H. et al., Proteomics 2008, 8, 1516–1527.