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Recently Published Articles
- Tunable Crosslinked Cell-Derived Extracellular Matrix Guides Cell Fate
Ramesh Subbiah, Mintai P. Hwang, Ping Du, Muhammad Suhaeri, Jun-Ha Hwang, Jeong-Ho Hong and Kwideok Park
Version of Record online: 25 AUG 2016 | DOI: 10.1002/mabi.201600280
The biophysical property of cell substrates plays a crucial role on cellular behaviors. Here, the use of cell-derived extracellular matrix (ECM), which closely reflects a complexity of natural ECM compositions and biophysical cues, is reported. A platform of varying ECM physiomechanical profiles is generated via crosslinking the ECM with different concentrations of genipin. Cell-specific responses to distinct ECM profiles have been demonstrated by examining how different types of cells respond to the crosslinked ECM microenvironments.
- Dual Enzyme-Triggered In Situ Crosslinkable Gelatin Hydrogels for Artificial Cellular Microenvironments
Bae Young Kim, Yunki Lee, Joo Young Son, Kyung Min Park and Ki Dong Park
Version of Record online: 25 AUG 2016 | DOI: 10.1002/mabi.201600312
A gelatin-based hydrogels formed through a dual enzyme-mediated crosslinking reaction using horseradish peroxidase (HRP) and glucose oxidase as an H2O2-generating enzyme to gradually supply a radical source in HRP-mediated crosslinking reaction is reported.
- Cell-Mediated Proteolytic Release of Growth Factors from Poly(Ethylene Glycol) Matrices
Stéphanie Metzger, Ulrich Blache, Philipp S. Lienemann, Maria Karlsson, Franz E. Weber, Wilfried Weber and Martin Ehrbar
Version of Record online: 22 AUG 2016 | DOI: 10.1002/mabi.201600223
A matrix metalloproteinase-sensitive streptavidin linker allows the efficient immobilization and cell-dependent release of biotinylated proteins for cell stimulation in 3D microenvironments. Using biotinylated bone morphogenetic protein (BMP)-2 as a proof of concept, cells are driven toward osteogenesis and show even higher osteogenic differentiation with the immobilized BMP-2 than with comparable amounts of growth factor presented in soluble form.
- Refolding of Aggregation-Prone ScFv Antibody Fragments Assisted by Hydrophobically Modified Poly(sodium acrylate) Derivatives
Nicolas Martin, Narciso Costa, Frank Wien, Françoise M. Winnik, Céline Ortega, Amaury Herbet, Didier Boquet and Christophe Tribet
Version of Record online: 22 AUG 2016 | DOI: 10.1002/mabi.201600213
Amphiphilic derivatives of poly(acrylicacid) are assessed for their ability to help chemical refolding of aggregation-prone scFv. Weak noncovalent associations with the polymers impart solubility to scFv and are compatible with recovery of a native-like conformation.
- Reducible Polyethylenimine Nanoparticles for Efficient siRNA Delivery in Corneal Neovascularization Therapy
Hyounkoo Han, Sohee Son, Sejin Son, Namho Kim, Ji Young Yhee, Jae Hyeop Lee, Jun-Sub Choi, Choun-Ki Joo, Hohyeon Lee, Duhwan Lee, Won Jong Kim, Sun Hwa Kim, Ick Chan Kwon, Hyuncheol Kim and Kwangmeyung Kim
Version of Record online: 9 AUG 2016 | DOI: 10.1002/mabi.201600051
NFκB-siRNA/reducible cationic polymer mediated ocular disease therapy shows significant antiangiogenesis effect without noticeable toxicity after subconjunctival injection into rat models. This study demonstrates that bioreducible cationic polymer can be a potent siRNA delivery carrier for treating various ocular diseases.