Macromolecular Bioscience

Cover image for Vol. 14 Issue 9

Early View (Online Version of Record published before inclusion in an issue)

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  1. 1 - 42
  1. Full Papers

    1. Sulfated Hyaluronan Influences the Formation of Artificial Extracellular Matrices and the Adhesion of Osteogenic Cells

      Alina Miron, Sandra Rother, Linda Huebner, Ute Hempel, Iris Käppler, Stephanie Moeller, Matthias Schnabelrauch, Dieter Scharnweber and Vera Hintze

      Article first published online: 12 SEP 2014 | DOI: 10.1002/mabi.201400292

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      The presence of sulfated glycosaminoglycans (GAG), like sulfated hyaluronan derivatives and chondroitin sulfate, during in vitro-fibrillogenesis of collagen type I alters the structure of the resulting artificial extracellular matrices. Besides, the GAG amount associated to collagen increases in a sulfation-dependent manner. Moreover, GAG sulfation influences the cytoskeletal arrangement of SaOS-2 cells which implies that adhesion-related intracellular signaling is affected.

    2. pH-Responsive Single Walled Carbon Nanotube Dispersion for Target Specific Release of Doxorubicin to Cancer Cells

      Moumita Ghosh, Sayanti Brahmachari and Prasanta Kumar Das

      Article first published online: 11 SEP 2014 | DOI: 10.1002/mabi.201400290

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      pH Responsive cholesterol based dipeptide carboxylates were synthesized as efficient dispersing agents for SWNTs in water. These highly dispersed nanotubes were used for the uploading of anticancer drug doxorubicin. The nanohybrid showed excellent pH responsive drug release at the extracellular tumorogenic environmental pH (6.0–6.5) and selectively kills cancer cells.

    3. Liposomal Drug Deposits in Poly(Dopamine) Coatings: Effect of Their Composition, Cell Type, Uptake Pathway Considerations, and Shear Stress

      Martin E. Lynge, Marina Fernandez-Medina, Almar Postma and Brigitte Städler

      Article first published online: 11 SEP 2014 | DOI: 10.1002/mabi.201400350

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      Different types of liposome-containing poly(dopamine)-based films were assembled and their interaction with adhering cells was assessed. The internalization of cargo was not affected by the presence of lipophilic dopamine conjugates in the lipid membrane but by the charge of the liposomes. Clathrin-mediated endocytosis was identified as uptake pathway. Shear stress had an impact on the interaction of adhering endothelial cells with the composite films.

    4. Sprayed Cells and Polyelectrolyte Films for Biomaterial Functionalization: The Influence of Physical PLL–PGA Film Treatments on Dental Pulp Cell Behavior

      Ivan V. Panayotov, Pierre-Yves Collart-Dutilleul, Hamideh Salehi, Marta Martin, Attila Végh, Jacques Yachouh, Boyan Vladimirov, Péter Sipos, Balázs Szalontai, Csilla Gergely and Frédéric J. G. Cuisinier

      Article first published online: 11 SEP 2014 | DOI: 10.1002/mabi.201400256

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      A good manufacturing practice (GMP)-compliant spraying method is described for the buildup of polyelectro-lyte multilayer (PEM) films constructed by poly-L-lysine (PLL) and poly-glutamic acid (PGA), onto which dental pulp cells are also deposited by spraying. The prepared PEM films were exposed to UV irradiation and drying/re-hydrating cycle. This procedure renders the films thicker, rougher and stiffer, enhancing dental pulp cell adhesion and proliferation.

    5. Bamboo Leaf-Like Micro-Nano Sheets Self-Assembled by Block Copolymers as Wafers for Cells

      Wen Zhu, Bo Peng, Jing Wang, Ke Zhang, Lixin Liu and Yongming Chen

      Article first published online: 10 SEP 2014 | DOI: 10.1002/mabi.201400283

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      Bamboo leaf-like micro-nano sheets are prepared via crystallization-driven self-assembly of poly(ethylene oxide)-b-poly(ϵ-caprolactone). The preparation is quite convenient within 1 h. These polymer sheets look like wafers for cells, and they do display a selective internalization to different cells. The macrophages show great interest in the wafers.

    6. Cell Motility on Polyethylene Glycol Block Copolymers Correlates to Fibronectin Surface Adsorption

      Peter J. F. Röttgermann, Samira Hertrich, Ida Berts, Max Albert, Felix J. Segerer, Jean-François Moulin, Bert Nickel and Joachim O. Rädler

      Article first published online: 10 SEP 2014 | DOI: 10.1002/mabi.201400246

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      Cell morphology and motility on artificial surfaces can be controlled by different degrees of surface PEGylations. Here, fibronectin incubation in three different PEG block copolymers is analyzed via neutron reflectivity. Fibronectin adsorbs in the vicinity of the PLL layer. The amount of fibronectin in the polymer layer is correlated to cell spreading and motility.

    7. Decomposition and Transformation of Pyrene-Derivative Micelles at Intracellular Milieu and Their Influence on Cytoviability

      Haisheng Wang, Wei Yu, Wenbo Zhang and Changyou Gao

      Article first published online: 10 SEP 2014 | DOI: 10.1002/mabi.201400338

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      Amphiphilic pyrene-containing random copolymers with pH-sensitive hydrazone bonds are synthesized. Spherical hydrolysable micelles formed by the amphiphilic copolymers at pH 6 are transformed into nanorods due to cleavage of the hydrazone bonds. The shape transformation is observed in pH 5.5 PBS too. The hydrolysable micelles cause higher toxicity to A549 cells than the non-hydrolysable ones without shape transformation.

    8. Preparation and Bioimaging Applications of AIE Dye Cross-linked Luminescent Polymeric Nanoparticles

      Meiying Liu, Xiqi Zhang, Bin Yang, Fengjie Deng, Yang Yang, Zhen Li, Xiaoyong Zhang and Yen Wei

      Article first published online: 8 SEP 2014 | DOI: 10.1002/mabi.201400262

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      We demonstrate that red luminescent polymeric nanoparticles can be prepared via combination of free radical polymerization and a ring-opening reaction using aggregation-induced emission dye as cross-linker. The good water dispersibility, size, enhanced fluorescence intensity, and excellent biocompatibility that these luminescent polymeric nanoparticles exhibit gives them good potential for biological imaging applications.

  2. Communications

    1. Collagen Surfaces Modified with Photo-Cleavable Polyethylene Glycol-Lipid Support Versatile Single-Cell Arrays of Both Non-adherent and Adherent Cells

      Shinya Yamahira, Satoshi Yamaguchi, Masahiro Kawahara and Teruyuki Nagamune

      Article first published online: 5 SEP 2014 | DOI: 10.1002/mabi.201400312

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      A smart-material surface for simply and rapidly preparing versatile single-cell arrays of both non-adherent and adherent cells was designed and constructed based on a photo-cleavable polyethylene glycol (PEG)-lipid. Two high-throughput image analysis applications were demonstrated on these PEG-lipid-based single cell arrays. In addition, extremely densely-packed single-cell arrays were successfully constructed.

  3. Full Papers

    1. In Vitro Synergistic Action of Geldanamycin- and Docetaxel-Containing HPMA Copolymer–RGDfK Conjugates Against Ovarian Cancer

      Nate Larson, Sarah Roberts, Abhijit Ray, Brandon Buckway, Darwin L. Cheney and Hamidreza Ghandehari

      Article first published online: 4 SEP 2014 | DOI: 10.1002/mabi.201400360

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      In vitro evaluation of combination therapy utilizing HPMA copolymer–RGDfK conjugates. Aminohexylgeldanamycin conjugates are more stable and exhibit slower drug release than docetaxel conjugates. Both conjugates demonstrate binding specifically to αvβ3 integrins. HPMA copolymer–RGDfK conjugates bearing aminohexylgeldanamycin and docetaxel induce cytotoxicity synergistically suggesting potential as combination therapy for ovarian cancer.

  4. Communications

    1. Microfluidic Synthesis of Pharmacologically Responsive Supramolecular Biohybrid Microgels

      Désirée Hövermann, Torsten Rossow, Raphael J. Gübeli, Sebastian Seiffert and Wilfried Weber

      Article first published online: 3 SEP 2014 | DOI: 10.1002/mabi.201400342

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      We describe the microfluidic synthesis of pharmacologically responsive biohybrid hydrogel particles synthesized from 8-arm poly(ethylene glycol) coupled to fluorescein and a fluorescein-specific humanized antibody fragment. Mixing the reactants in a microfluidic device results in narrowly dispersed microgels that can be degraded selectively by addition of fluorescein to disrupt the antigen–antibody ties.

  5. Reviews

    1. Polymeric Anti-HIV Therapeutics

      Maarten Danial and Harm-Anton Klok

      Article first published online: 3 SEP 2014 | DOI: 10.1002/mabi.201400298

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      Anti-HIV therapeutics have enabled to improve the lives of many infected individuals and shifted the prognosis to a chronic but manageable disease. In parallel to the development of small molecule drug therapeutics, profound interest and efforts have been made to develop polymeric anti-HIV therapeutics. An overview of these macromolecular therapeutics is presented herein.

  6. Full Papers

    1. A Combined Micelle and Poly(Serinol Hexamethylene Urea)-Co-Poly(N-Isopropylacrylamide) Reverse Thermal Gel as an Injectable Ocular Drug Delivery System

      Amin Famili, Malik Y. Kahook and Daewon Park

      Article first published online: 3 SEP 2014 | DOI: 10.1002/mabi.201400250

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      A poorly-soluble ocular corticosteroid is encapsulated within polymeric micelles, which are mixed with a novel reverse thermal gel to form an injectable long-term drug delivery system. This combined system improves release kinetics of the drug over micelles or reverse thermal gel alone by eliminating burst release behavior and extending the timeframe of drug release.

    2. Spatiotemporally Programmable Surface Engineered Nanoparticles for Effective Anticancer Drug Delivery

      Arsalan Ahmed, Hongliang Yu, Dingwang Han, Jingwei Rao, Yin Ding and Yong Hu

      Article first published online: 2 SEP 2014 | DOI: 10.1002/mabi.201400228

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      Reducible stealthy hybrid nanoparticles are fabricated. The obtained nanoparticles have a sandwich structure consisting of a long circulating outermost layer, a targeting middle layer, and a hydrophobic innermost core. The outermost layer detaches from the nanoparticles in the tumor tissue. Targeting ligands are used to strengthen the interaction between nanoparticles and tumor cells, and the inner core encapsulates and protects the drug from non-specific interactions.

  7. Communications

    1. Nanostructured Gel Scaffolds for Osteogenesis through Biological Assembly of Biopolymers via Specific Nucleobase Pairing

      Ming Fan, Jingxuan Yan, Huaping Tan, Dandan Ben, Qiuling He, Zhongwei Huang and Xiaohong Hu

      Article first published online: 2 SEP 2014 | DOI: 10.1002/mabi.201400320

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      A nanostructured gel scaffold basing cellulose and heparin was developed for targeted BMP-2 delivery and in vitro osteogenesis. The gel scaffold was attributed to the mechanism of the Watson–Crick base pairing via intermolecular hydrogen bonding between thymine and adenine.

  8. Full Papers

    1. Structurally Distinct Hybrid Polymer/Lipid Nanoconstructs Harboring a Type-I Ribotoxin as Cellular Imaging and Glioblastoma-Directed Therapeutic Vectors

      M. Sheikh Mohamed, Srivani Veeranarayanan, Ankur Baliyan, Aby Cheruvathoor Poulose, Yutaka Nagaoka, Hiroaki Minegishi, Seiki Iwai, Yasuhiro Shimane, Yasuhiko Yoshida, Toru Maekawa and D. Sakthi Kumar

      Article first published online: 2 SEP 2014 | DOI: 10.1002/mabi.201400248

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      A multicomponent polymeric/lipid nano-construct encompassing a plant toxin and imaging dye was utilized to specifically curb the proliferation tendencies of glioblastoma in addition to suppressing the anti-apoptotic survivin and membrane interaction vinculin proteins. The cytocompatibility and multifunctionality of the nanoparticles significantly projects their prospective theranostic potential.

    2. Novel Poly(Ethylene Oxide)-b-Poly(Propylene Oxide) Copolymer-Glucose Conjugate by the Microwave-Assisted Ring Opening of a Sugar Lactone

      Romina J. Glisoni and Alejandro Sosnik

      Article first published online: 27 AUG 2014 | DOI: 10.1002/mabi.201400235

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      The surface modification of Pluronic® F127 polymeric micelles with glucose residues by means of a fast and efficient ring opening reaction of gluconolactone leads to their recognition by a soluble vegetal lectin, Concanavalin A, and their subsequent agglutination.

    3. Stabilization of Gelatin Nanoparticles Without Crosslinking

      Saeed Ahmad Khan and Marc Schneider

      Article first published online: 26 AUG 2014 | DOI: 10.1002/mabi.201400214

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      Crosslinking free preparation of gelatin nanoparticles is described, offering a flexible drug carrier system for hydrophilic materials. The structural integrity of gelatin nanoparticles is kept intact by coating them with Eudragit® E 100 forming hybrid nanospheres. For preparation, a hybrid technique of nanoprecipitation-emulsion solvent evaporation is employed.

    4. Synthesis of Homopolypeptides by Aminolysis Mediated by Proteases Encapsulated in Silica Nanospheres

      Peter J. Baker, Siddharth V. Patwardhan and Keiji Numata

      Article first published online: 25 AUG 2014 | DOI: 10.1002/mabi.201400295

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      The encapsulation of three different proteases (bromelain, papain, and trypsin) in bioinspired silica enhances their thermal stability for both hydrolysis and aminolysis reactions. The enhanced thermal activity resulted in increased production of poly-L-leucine, poly-L-alanine, and poly-L-valine by protease-mediated aminolysis at 50 °C.

    5. Boronic Acid Shell-Crosslinked Dextran-b-PLA Micelles for Acid-Responsive Drug Delivery

      Ziwei Zhao, Xuemei Yao, Zhe Zhang, Li Chen, Chaoliang He and Xuesi Chen

      Article first published online: 21 AUG 2014 | DOI: 10.1002/mabi.201400251

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      3-carboxy-5-nitrophenylboronic acid (CNPBA) shell-crosslinked micelles based on amphiphilic dextran-block-polylactide (Dex-b-PLA) are prepared and used for efficient intracellular drug delivery. The pH-responsive biodegradable CNPBA-Dex-b-PLA can efficiently load and deliver DOX into tumor cells and enhance the inhibition of cellular proliferation in vitro, providing a favorable platform as a drug delivery system for cancer therapy.

    6. Functional Brush-Decorated Poly(globalide) Films by ARGET-ATRP for Bioconjugation

      Zeliha Ates, Fabrice Audouin, Amy Harrington, Brendan O'Connor and Andreas Heise

      Article first published online: 21 AUG 2014 | DOI: 10.1002/mabi.201400282

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      Poly(acrylic) acid brushes were grafted from polyester films obtained from the unsaturated macrolactone globalide by ARGET ATRP of tert.-butyl acrylate. After mild in situ deprotection high-density acrylic acid functional films were obtained while avoiding polyester hydrolysis. The conjugation with green fluorescent protein (GFP) and an enzyme was successfully carried out retaining the enzymatic activity.

    7. Monodisperse Carboxyl-Functionalized Poly(Ethylene Glycol)-Coated Magnetic Poly(Glycidyl Methacrylate) Microspheres: Application to the Immunocapture of β-Amyloid Peptides

      Daniel Horák, Helena Hlídková, Mohamed Hiraoui, Myriam Taverna, Vladimír Proks, Eliška Mázl Chánová, Claire Smadja and Zdenka Kučerová

      Article first published online: 21 AUG 2014 | DOI: 10.1002/mabi.201400249

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      Monodisperse magnetic poly(glycidyl methacrylate) microspheres coated with α-amino-ω-methoxy-PEG5000/α-amino-ω-Boc-NH-PEG5000 substantially reduced non-specific protein adsorption. Anti-Aβ 6E10-immobilized microspheres specifically captured β-amyloid peptide (Aβ) from model solutions. Identification and evaluation of small changes in Aβ levels in cerebrospinal fluid is of crucial importance for early detection of Alzheimer's disease.

  9. Communication

    1. Micropatterning Electrospun Scaffolds to Create Intrinsic Vascular Networks

      Eric M. Jeffries, Shintaro Nakamura, Kee-Won Lee, Jimmy Clampffer, Hiroyuki Ijima and Yadong Wang

      Article first published online: 20 AUG 2014 | DOI: 10.1002/mabi.201400306

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      Engineered microvasculature that effectively perfuses the parenchyme and directly integrates with host circulation remains the challenge of organ engineering. Described is a method for patterning microvascular structures in electrospun scaffolds using water-soluble templates produced by fused deposition modeling. The fibrous nature mimicks extracellular matrix and permits nutrient diffusion. Additionally, scaffolds are biodegradable and suturable. In vitro endothelialization demonstrates feasible vascularization.

  10. Full Papers

    1. Electrospun Honeycomb as Nests for Controlled Osteoblast Spatial Organization

      Salima Nedjari, Sandy Eap, Anne Hébraud, Corinne R. Wittmer, Nadia Benkirane-Jessel and Guy Schlatter

      Article first published online: 19 AUG 2014 | DOI: 10.1002/mabi.201400226

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      PCL and PLA nanofibrous scaffolds with honeycomb micro-patterns are prepared by electrospinning. It is shown that the relief of the scaffolds depends on the distribution of fiber diameters. Mimicking hemi-osteons, cell gathering is observed inside the PCL honeycomb fibrous nests with a characteristic size greater than 80 μm.

    2. An Orthogonal Click-Chemistry Approach to Design Poly(glycerol monomethacrylate)-based Nanomaterials for Controlled Immunostimulation

      Lakshminarayanan Ragupathy, Douglas G. Millar, Nicola Tirelli and Francesco Cellesi

      Article first published online: 18 AUG 2014 | DOI: 10.1002/mabi.201400146

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      Immune active polymers based on poly(glycerol monomethacrylate) (PGMMA) are presented. Propargyl-terminated PGMMAs, modified with dangling acrylate groups, are conjugated with thiomannose through Michael-type addition. Orthogonal azide-alkyne cycloaddition can be used to prepare a final traceable nanomaterial. Depending on the mannose content, these polymers can activate dendritic cells, stimulating cytokine (TNFα) and surface activation marker (CD40) expression.

    3. Telechelic Poly(2-oxazoline)s with a Biocidal and a Polymerizable Terminal as Collagenase Inhibiting Additive for Long-Term Active Antimicrobial Dental Materials

      Christoph P. Fik, Stefan Konieczny, David H. Pashley, Christian J. Waschinski, Reinhild S. Ladisch, Ulrich Salz, Thorsten Bock and Joerg C. Tiller

      Article first published online: 8 AUG 2014 | DOI: 10.1002/mabi.201400220

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      A dental adhesive is rendered antimicrobially contact-active by an additive based on poly(2-methyloxazoline) equipped with a biocidal and a polymerizable end group. The polymeric additive also kills cariogenic bacteria in dentin tubuli and inhibits collagenases, such as human MMP that naturally adhere to dental tissue for preventing dentin degradation below a dental restoration.

    4. Stable Biochemically Micro-patterned Hydrogel Layers Control Specific Cell Adhesion and Allow Long Term Cyclic Tensile Strain Experiments

      Alexandra M. Greiner, Peter Hoffmann, Kristina Bruellhoff, Simon Jungbauer, Joachim P. Spatz, Martin Moeller, Ralf Kemkemer and Jürgen Groll

      Article first published online: 6 AUG 2014 | DOI: 10.1002/mabi.201400261

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      PDMS coated with sP(EO-stat-PO) is patterned with fibronectin by micro-contact printing and used for studies with human dermal fibroblasts exposed to uniaxial cyclic tensile strain (CTS). The coating has long term specific protein and cell anti- and pro-adhesive properties. The cells align along the fibronectin lines and orient accordingly their actin fibers independently of the direction of CTS application.

    5. A Novel Crosslinked Hyaluronic Acid Nanogel for Drug Delivery

      Sílvia Santos Pedrosa, Catarina Gonçalves, Laurent David and Miguel Gama

      Article first published online: 4 AUG 2014 | DOI: 10.1002/mabi.201400135

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      A simple two-step method is developed for the synthesis of a redox sensitive hyaluronic acid nanogel. Hyaluronic acid is chemically grafted with a thiolated alkyl chain that allows the reticulation of the self assembled nanogel by dissulfide bond. The nanogel presents high stability and drug loading efficiency.

    6. Rapid Flow Through Immunoassay for CRP Determination Based on Polyethylene Filters Modified with ω-Aminocellulose Carbamate

      Thomas Elschner, Friedrich Scholz, Peter Miethe and Thomas Heinze

      Article first published online: 4 AUG 2014 | DOI: 10.1002/mabi.201400179

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      A new covalent antibody immobilization method based on ω-aminocellulose carbamate coatings is investigated. The flow through immunoassay for CRP determination possesses a very low limit of detection (5 ng CRP mL−1) and a high precision (CV < 9.1%).

    7. 18F-Radiolabeling, Preliminary Evaluation of Folate-pHPMA Conjugates via PET

      Hanno Schieferstein, Annette Kelsch, Achim Reibel, Kaloian Koynov, Matthias Barz, Hans-Georg Buchholz, Nicole Bausbacher, Oliver Thews, Rudolf Zentel and Tobias L. Ross

      Article first published online: 4 AUG 2014 | DOI: 10.1002/mabi.201400200

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      For two new 18F-labeled pHPMA copolymers, which are functionalized with folate units, active targeting can be proved in vivo (Walker carcinoma) by blockade studies.

    8. Bioreducible Branched Polyethyleneimine Derivatives Physically Loaded with Hydrophobic Pheophorbide A: Preparation, Characterization, and Light-Induced Cytotoxicity

      Hana Cho, Li Li, You Han Bae, Kang Moo Huh and Han Chang Kang

      Article first published online: 1 AUG 2014 | DOI: 10.1002/mabi.201400145

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      Branched types of polyethyleneimine (bPEI) derivatives can load hydrophobic photosensitizers in neutral or basic pH-tuned hydrophobic cavities. However, drug release from bPEI derivatives is not activated by acidic pH but by cytosol-specific triggered biodegradation. This mechanism results in light-mediated cytotoxic effects when photosensitizer-loaded reducible bPEI derivatives are used that are not seen when non-reducible bPEI derivatives are used.

    9. Nanoparticle/Polymer Assembled Microcapsules with pH Sensing Property

      Pan Zhang, Xiaoxue Song, Weijun Tong and Changyou Gao

      Article first published online: 1 AUG 2014 | DOI: 10.1002/mabi.201400259

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      The dual-labeled microcapsules via nanoparticle/polymer assembly based on polyamine–salt aggregates are fabricated for the ratiometric intracellular pH sensing. The microcapsules are internalized by several kinds of cells naturally, and after being internalized they are all located in acidic organelles. Negatively charged drugs are easily encapsulated into capsules.

    10. Hybrid Polyglycerols with Long Blood Circulation: Synthesis, Biocompatibility, and Biodistribution

      Muhammad Imran ul-haq, Benjamin F. L. Lai and Jayachandran N. Kizhakkedathu

      Article first published online: 11 JUL 2014 | DOI: 10.1002/mabi.201400152

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      Hybrid polyglycerols (inline image ≈ 100 kDa) with different compositions (15–80 mol% linear polyglycerol) with excellent blood and cell compatibility are synthesized. The polymers show long blood circulation with minimal organ accumulation in mice and are attractive for the development of multifunctional drug delivery systems.

    11. Cell–Microsphere Constructs Formed with Human Adipose-Derived Stem Cells and Gelatin Microspheres Promotes Stemness, Differentiation, and Controlled Pro-Angiogenic Potential

      Anjaneyulu Kodali, Thiam Chye Lim, David Tai Leong and Yen Wah Tong

      Article first published online: 1 JUL 2014 | DOI: 10.1002/mabi.201400094

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      Three-dimensional cell–microsphere constructs formed with gelatin microspheres are found to be suitable platforms for the growth and differentiation of adipose-derived stem cells (ADSCs). These constructs also tend to preserve the stemness properties of the ADSCs along with enhancing their pro-angiogenic properties.

    12. Toward Anticancer Immunotherapeutics: Well-Defined Polymer–Antibody Conjugates for Selective Dendritic Cell Targeting

      Kristof Tappertzhofen, Monika Bednarczyk, Kaloian Koynov, Matthias Bros, Stephan Grabbe and Rudolf Zentel

      Article first published online: 30 JUN 2014 | DOI: 10.1002/mabi.201400190

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      The synthesis of multifunctionalizable semitelechelic maleimide-modifided HPMA based polymers of narrow dispersity that can be conjugated to anti-DEC-205 antibodies affording “star-like” topologies is described. Primary in vitro studies show their high potential to target dendritic cells (DCs). This opens the opportunity to present tumor antigens to DCs and thus to induce antitumor immune responses.

    13. Enzymatic Synthesis of Dendritic Amphoteric α-Glucans by Thermostable Phosphorylase Catalysis

      Yusei Takata, Riko Shimohigoshi, Kazuya Yamamoto and Jun-Ichi Kadokawa

      Article first published online: 30 JUN 2014 | DOI: 10.1002/mabi.201400204

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      Enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends is performed by phosphorylase catalysis, which shows inherent isoelectric points and assembling/disassembling behaviors in water in accordance of the pH values.

    14. Synthesis of Functionalized Poly(ester carbonate) with Laminin-Derived Peptide for Promoting Neurite Outgrowth of PC12 cells

      Dongming Xing, Lie Ma and Changyou Gao

      Article first published online: 25 JUN 2014 | DOI: 10.1002/mabi.201400186

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      Laminin-derived peptide functionalized poly(ester carbonate) (PEC) is synthesized to promote neurite outgrowth of PC12 cells. The maleimide-functionalized PECs are clicked with various thiol-containing molecules. The CQAASIKVAV-grafted PEC could support cell proliferation and neurite outgrowth. Therefore, the maleimide-functionalized PECs can serve as a versatile platform for diverse functionalization and have comprehensive potential in biomedical applications.

    15. Hydrophobic Polyalanine Modified Hyperbranched Polyethylenimine as High Efficient pDNA and siRNA Carrier

      Zhaopei Guo, Huayu Tian, Lin Lin, Jie Chen, Chaoliang He, Zhaohui Tang and Xuesi Chen

      Article first published online: 20 JUN 2014 | DOI: 10.1002/mabi.201400044

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      Successful gene therapy for cancer and various human genetic diseases treatment relies on high efficient gene carriers. But for most carriers, either DNA or RNA can be effectively delivered. Here, amphiphilic copolymers polyethylenimine-polyalanine, with good biocompatibility, are prepared for high efficient transfection both in DNA and RNA.

    16. Star-Shape Redox-Responsive PEG-Sheddable Copolymer of Disulfide-Linked Polyethylene Glycol-Lysine-di-Tocopherol Succinate for Tumor-Triggering Intracellular Doxorubicin Rapid Release: Head-to-Head Comparison

      Xiaoyu Ai, Jin Sun, Lu Zhong, Chunnuan Wu, Handong Niu, Tao Xu, He Lian, Xiaopeng Han, Guolian Ren, Wenya Ding, Jia Wang, Xiaohui Pu and Zhonggui He

      Article first published online: 20 JUN 2014 | DOI: 10.1002/mabi.201400149

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      Self-assembled redox-responsive nanomicelles are endocytosed into tumor cells. Then, disulfide bonds in micelles are cleaved and poly(ethylene glycol) (PEG) hydration shell is shed, induced by the high glutathione concentration. The nanomicelles are disassembled and the loaded doxorubicin (DOX) is released into the plasma and translocates into the nucleus to exert its anticancer effect.

    17. Protease-Sensitive PEG Hydrogels Regulate Vascularization In Vitro and In Vivo

      Marina Vigen, Jacob Ceccarelli and Andrew J. Putnam

      Article first published online: 18 JUN 2014 | DOI: 10.1002/mabi.201400161

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      Co-encapsulation of endothelial cells and supportive fibroblasts within proteolyzable poly(ethylene glycol) (PEG)-based hydrogels led to vascular morphogenesis in vitro that was robust to changes in crosslinking peptide identity, but was significantly attenuated by gel crosslinking and MMP inhibition. In vivo, perfused vasculature formed from transplanted cells in all gel types, and vascularization was not attenuated in more crosslinked gels.

    18. Introducing PeptoPlexes: Polylysine-block-Polysarcosine Based Polyplexes for Transfection of HEK 293T Cells

      Philipp Heller, Alexander Birke, David Huesmann, Benjamin Weber, Karl Fischer, Angelika Reske-Kunz, Matthias Bros and Matthias Barz

      Article first published online: 18 JUN 2014 | DOI: 10.1002/mabi.201400167

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      The synthesis, characterization, and application of polypeptide-polypeptoid block copolymers and polyplexes thereof based on the body's own amino acids sarcosine and lysine are presented. The pathway to PLys-block-PSar and the use of the synthesized block ionomers for the formation of polyplexes (PeptoPlexes) and for first transfection studies in HEK 293T cells are described.

  11. Communication

    1. Zwitterionic Red Fluorescent Polymeric Nanoparticles for Cell Imaging

      Haiyin Li, Xiqi Zhang, Xiaoyong Zhang, Bin Yang, Yang Yang, Zengfang Huang and Yen Wei

      Article first published online: 13 JUN 2014 | DOI: 10.1002/mabi.201400223

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      Cross-linked zwitterionic red fluorescent copolymers are fabricated by incorporating phospholipid monomer and aggregation-induced emission dye-based dimer via reversible addition–fragmentation transfer polymerization. These cross-linked polymers are easy to self-assembly into uniform nanoparticles that show high water dispersibility, intense red fluorescence, and excellent biocompatibility, making them promising for cell imaging applications.

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