Macromolecular Bioscience

Cover image for Vol. 14 Issue 11

Early View (Online Version of Record published before inclusion in an issue)

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  1. 1 - 41
  1. Full Papers

    1. Engineering a Freestanding Biomimetic Cardiac Patch Using Biodegradable Poly(lactic-co-glycolic acid) (PLGA) and Human Embryonic Stem Cell-derived Ventricular Cardiomyocytes (hESC-VCMs)

      Yin Chen, Junping Wang, Bo Shen, Camie W. Y. Chan, Chaoyi Wang, Yihua Zhao, Ho N. Chan, Qian Tian, Yangfan Chen, Chunlei Yao, I-Ming Hsing, Ronald A. Li and Hongkai Wu

      Article first published online: 25 NOV 2014 | DOI: 10.1002/mabi.201400448

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      A facile method for engineering a freestanding biomimetic cardiac patch using biodegradable poly(lactic-co-glycolic acid) (PLGA) and stem cell-derived cardiomyocytes is presented. The biomimetic cardiac patch fabricated with microgrooved PLGA film recapitulates the anisotropic electrophysiological feature of native cardiac tissue and is believed to be applicable in the treatment of myocardial infarction.

  2. Invited Reviews

    1. Elastin-Like Polypeptide Based Nanoparticles: Design Rationale Toward Nanomedicine

      Ferdinanda C. M. Smits, Bastiaan C. Buddingh, Mark B. van Eldijk and Jan C. M. van Hest

      Article first published online: 19 NOV 2014 | DOI: 10.1002/mabi.201400419

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      Elastin-like polypeptides (ELPs) are biocompatible, temperature responsive and can be made with high sequence control. This makes ELP-based materials very interesting for their use towards nanomedicine applications. Many ELP-based systems that show self-assembly into nanoparticles have been developed. This review will give an overview of the ELP-based block copolymers capable of nanoparticle formation and their design parameters.

  3. Full Papers

    1. Multiphase Protein Microgels

      Ulyana Shimanovich, Yang Song, Jasna Brujic, Ho Cheung Shum and Tuomas P. J. Knowles

      Article first published online: 19 NOV 2014 | DOI: 10.1002/mabi.201400366

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      We show that the characteristics of aqueous two-phase systems can be exploited in combination with protein self-assembly for controlling aggregation and structuring of fibrillar proteins into 3D core–shell microgels.

    2. A Cationic Poly(2-oxazoline) with High In Vitro Transfection Efficiency Identified by a Library Approach

      Alexandra C. Rinkenauer, Lutz Tauhardt, Felix Wendler, Kristian Kempe, Michael Gottschaldt, Anja Traeger and Ulrich S. Schubert

      Article first published online: 18 NOV 2014 | DOI: 10.1002/mabi.201400334

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      An 18-membered library of cationic poly(2-oxazoline)-based copolymers has been prepared. The influence of different polymer parameters, namely the polymer side chain hydrophobicity and the type and content of amino groups, on the pDNA condensation, the transfection efficiency, the cytotoxicity, and cellular membrane interaction as well as the size, charge, and stability of the polyplexes was studied and first structure–property relationships elucidated.

    3. Protein Compatibility of Selected Cross-linking Reactions for Hydrogels

      Nadine Hammer, Ferdinand P. Brandl, Susanne Kirchhof, Viktoria Messmann and Achim M. Goepferich

      Article first published online: 14 NOV 2014 | DOI: 10.1002/mabi.201400379

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      Despite years of research, finding cross-linking reactions for hydrogels that are compatible with incorporated proteins is still challenging. The authors, therefore, investigate side reactions between a model protein and methoxypolyethylene glycol functionalized with maleimide, vinyl sulfone, acrylamide, thiol, and furyl moieties. The extent of protein modification was found to depend on the cross-linking agent and the pH during incubation.

    4. Novel Cationic Triblock Copolymer of Poly[2-(dimethylamino)ethyl methacrylate]-block-poly(β-amino ester)-block-poly[2-(dimethylamino)ethyl methacrylate]: A Promising Non-Viral Gene Delivery System

      Rosemeyre A. Cordeiro, Dina Farinha, Nuno Rocha, Arménio C. Serra, Henrique Faneca and Jorge F. J. Coelho

      Article first published online: 14 NOV 2014 | DOI: 10.1002/mabi.201400424

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      An efficient block copolymer non-viral vector composed by poly[2-(dimethylamino)ethyl methacrylate] and poly(β-amino ester) for plasmid DNA delivery. The new cationic block copolymers of poly[2-(dimethylamino)ethyl methacrylate]-b-poly(β-amino ester)-b-poly[2-(dimethylamino)ethyl methacrylate] efficiently bind pDNA and show high transfection activity in HeLa and COS-7 cell lines compared to bPEI and TurboFect™ polymeric non-viral vector standards.

    5. Thermoresponsive Self-Assembly of Nanostructures from a Collagen-Like Peptide-Containing Diblock Copolymer

      Tianzhi Luo, Lirong He, Patrick Theato and Kristi L. Kiick

      Article first published online: 13 NOV 2014 | DOI: 10.1002/mabi.201400358

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      Well-defined vesicles with a diameter of approximately 100 nm were produced from temperature-triggered self-assembly from a collagen-like peptide containing thermoresponsive diblock conjugates, driven by the coil/globule conformational transition of the PDEGMEMA building block above its LCST. The incorporation of CLP domains in these nanostructures offers opportunities for the selective targeting of collagen-containing matrices.

    6. Targeted Delivery of Vaccine to Dendritic Cells by Chitosan Nanoparticles Conjugated with a Targeting Peptide Ligand Selected by Phage Display Technique

      Su-Na Jung, Sang-Kee Kang, Guen-Hye Yeo, Hai-Ying Li, Tao Jiang, Jae-Woon Nah, Jin-Duck Bok, Chong-Su Cho and Yun-Jaie Choi

      Article first published online: 13 NOV 2014 | DOI: 10.1002/mabi.201400352

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      Delivering antigen to dendritic cells (DCs) is a promising strategy for enhancing vaccine efficacy. A novel DC-targeting peptide ligand is isolated by phage display technique. DC-targeting peptide conjugated chitosan nanoparticles (TPC-NPs) are designed and evaluated for their targeting efficiency to DCs. Furthermore, immunization of mice with antigen-carrying TPC-NPs induces strong and balanced immune responses, demonstrating that TPC-NPs can further improve the immunogenicity of vaccine.

    7. Clustered Nanocarriers: The Effect of Size on the Clustering of CCMV Virus-Like Particles With Soft Macromolecules

      Martijn Verwegen and Jeroen J. L. M. Cornelissen

      Article first published online: 12 NOV 2014 | DOI: 10.1002/mabi.201400326

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      Clusters of virus-like particles are potentially interesting biomaterials that can find application in drug delivery, imaging, etc. This clustering can be induced by positively charged macromolecules, of which we here show that the macromolecular topology in combination with the symmetry of the virus-like particle are crucial parameters.

    8. Micellization of Antineoplastic Agent to Significantly Upregulate Efficacy and Security

      Chen Li, Weiguo Xu, Jianxun Ding, Ying Zhang, Jincheng Wang, Xiuli Zhuang and Xuesi Chen

      Article first published online: 7 NOV 2014 | DOI: 10.1002/mabi.201400356

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      Doxorubicin-incorporated micelle is prepared by noncovalent interaction-assissed nanoprecipitation of drug and methoxy poly(ethylene glycol)-block-oligo(D,L-leucine). The loading micelle exhibits enhanced cellular interalization and antiproliferative activity in vitro, improved intratumoral distribution, and upregulated antitumor efficacy and security in vivo, which all indicate its great potential for smart delivery of antineoplastic agents.

    9. Diblock Copolymers of Polyethylene Glycol and a Polymethacrylamide with Side-Chains Containing Twin Ortho Ester Rings: Synthesis, Characterization, and Evaluation as Potential pH-Responsive Micelles

      Xiaojing Zhou, Shi Luo, Rupei Tang, Rui Wang and Jun Wang

      Article first published online: 5 NOV 2014 | DOI: 10.1002/mabi.201400395

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      Block copolymers, PEG-b-PMEA, bearing twin five-membered ortho ester pendants, formed micelles with pH-responsive solubility as a result of accelerated release of payload. The micelles were compatible with cells, and paclitaxel-loaded micelles killed cancer cells with potency equivalent to free drug, suggesting potential use as effective nano-carriers for anticancer drugs without toxic side-effects.

    10. Effect of Amphiphilic PCL–PEG Nano-Micelles on HepG2 Cell Migration

      Yang Shen, Mengtian Leng, Hongchi Yu, Qin Zhang, Xianglin Luo, Hans Gregersen, Guixue Wang and Xiaoheng Liu

      Article first published online: 4 NOV 2014 | DOI: 10.1002/mabi.201400376

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      Nano-sized polymer micelles attract attentions to their biocompatibility and biodegradability in antitumor therapy. However, few investigations focus on exploring the effect of internalized nano-micelles on the cancer cells migration. We demonstrated that amphiphilic PCL–PEG NPs-induced cell migration depended on integrin-FAK-Rho GTPases pathway, which expected to evoke more attentions in the design of drug delivery systems based on polymer micelles.

    11. Degradable Glycine-Based Photo-Polymerizable Polyphosphazenes for Use as Scaffolds for Tissue Regeneration

      Sandra Rothemund, Tamara B. Aigner, Aitziber Iturmendi, Maria Rigau, Branislav Husár, Florian Hildner, Eleni Oberbauer, Martina Prambauer, Gbenga Olawale, Reinhard Forstner, Robert Liska, Klaus R. Schröder, Oliver Brüggemann and Ian Teasdale

      Article first published online: 30 OCT 2014 | DOI: 10.1002/mabi.201400390

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      Hydrolytically degradable, porous matrices are prepared via a mild and simple photopolymerization of functionalized poly(organo)phosphazenes. The modular system allows for facile surface functionalization and property tuning. The novel materials are biocompatible and have tunable degradation rates to non-toxic small molecules and thus are promising for use as synthetic scaffolds for tissue regeneration.

    12. Substrate-induced PC12 Cell Differentiation Without Filopodial, Lamellipodial Activity or NGF Stimulation

      Guillaume Lamour, Sylvie Souès and Ahmed Hamraoui

      Article first published online: 28 OCT 2014 | DOI: 10.1002/mabi.201400323

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      PC12 cells are driven to extend neurites through cell-substrate interactions only, that is, without nerve growth factor (NGF) stimulation. Actin polymerization is destabilized by treating the cells with cytochalasin. As a result the cells are deprived of both filopodia and lamellipodia. However, they extend neurites continuously. This shows that neither filopodia nor lamellipodia are required to trigger substrate-induced neuronal differentiation of PC12 cells.

    13. Poly(anhydride-ester) and Poly(N-vinyl-2-pyrrolidone) Blends: Salicylic Acid-Releasing Blends with Hydrogel-Like Properties that Reduce Inflammation

      Michelle A. Ouimet, Renata Fogaça, Sabrina S. Snyder, Sameer Sathaye, Luiz H. Catalani, Darrin J. Pochan and Kathryn E. Uhrich

      Article first published online: 21 OCT 2014 | DOI: 10.1002/mabi.201400238

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      Hydrogels-like polymer blends that combine the biocompatibility and hydrophilicity of PVP with controlled release of salicylic acid to reduce inflammation are presented. These copolymer blends substantially reduce the inflammatory cytokine, TNF-α, in vitro and indicate no cytotoxic or anti-proliferative effects. Based upon the unique combination, these blended polymer films show great potential for wound healing.

    14. Multi-Technique Characterization of Poly-L-lysine Dendrigrafts–Cu(II) Complexes for Biocatalysis

      Jean-Christophe Rossi, Barbara Maret, Kevin Vidot, Jean-Patrick Francoia, Michela Cangiotti, Susanna Lucchi, Concetta Coppola and Maria Francesca Ottaviani

      Article first published online: 20 OCT 2014 | DOI: 10.1002/mabi.201400341

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      Significant structural features of poly-L-lysine dendrigrafts (DGLs) – Cu(II) complexes are characterized to implement the use of these complexes as biocatalysts. The agreement and complementarity among EPR, potentiometric titration, UV-Vis and CD measurements provide an exhaustive view of the Cu(II)-DGL complexation behavior as a function of DGL generation, relative concentrations, and pH.

  4. Feature Articles

    1. Thermoresponsive Nanodevices in Biomedical Applications

      Julián Bergueiro and Marcelo Calderón

      Article first published online: 16 OCT 2014 | DOI: 10.1002/mabi.201400362

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      Thermoresponsive nanodevices are one of the most promising systems in nanomedicine. Due to new building blocks, a number of promising smart systems have been rapidly developed in response to the needs of nanomedicine. This article reviews the innovations in this field from the last four years.

  5. Full Papers

    1. Blending of Diblock and Triblock Copolypeptide Amphiphiles Yields Cell Penetrating Vesicles with Low Toxicity

      April R. Rodriguez, Uh-Joo Choe, Daniel T. Kamei and Timothy J. Deming

      Article first published online: 16 OCT 2014 | DOI: 10.1002/mabi.201400348

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      Diblock and triblock copolypeptides were blended to form vesicle populations with uniform compositions. Mixing of cationic and non-ionic amphiphiles in these vesicles gave optimized properties of good cellular uptake while maintaining minimal cytotoxicity. Copolypeptide mixing is a promising strategy to provide useful functionality without requiring the use of complicated synthesis procedures.

    2. Glycosylated Star Polypeptides from NCA Polymerization: Selective Binding as a Function of Degree of Branching and Glycosylation

      Mark Byrne, Robert Mildner, Henning Menzel and Andreas Heise

      Article first published online: 15 OCT 2014 | DOI: 10.1002/mabi.201400371

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      Poly(glutamic acid) star polymers with up to 64 arms were synthesized via N-carboxy-anhydride (NCA) polymerization from dendritic initiators. Glycosylation afforded star-shaped glyco- peptides with varying degrees of sugar conjugation. The secondary structure and the ability to selectively bind to lectin ConA was found to be dependent on sugar content and polymer structure, respectively.

    3. Porous Polylactic Acid–Silica Hybrids: Preparation, Characterization, and Study of Mesenchymal Stem Cell Osteogenic Differentiation

      Christos Pandis, Sara Trujillo, Joana Matos, Sara Madeira, Joaquín Ródenas-Rochina, Sotiria Kripotou, Apostolos Kyritsis, João F. Mano and José Luis Gómez Ribelles

      Article first published online: 10 OCT 2014 | DOI: 10.1002/mabi.201400339

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      Polymer/silica porous hybrids are prepared by a novel strategy using sol–gel reactions inside the pores of polylactic acid (PLA) membranes. The link between organic and inorganic phase is achieved by functionalizing PLA and using glycidoxypropyltrimethoxysilane (GPTMS) as coupling agent. The adjustment of silica precursors ratio allows modifying the thermomechanical properties. The proliferation and osteoblastic differentiation potential is studied.

    4. Biocompatible, Biodegradable and Porous Liquid Crystal Elastomer Scaffolds for Spatial Cell Cultures

      Anshul Sharma, Abdollah Neshat, Cory J. Mahnen, Alek d. Nielsen, Jacob Snyder, Tory L. Stankovich, Benjamin G. Daum, Emily M. LaSpina, Gabrielle Beltrano, Yunxiang Gao, Shuo Li, Byung-Woo Park, Robert J. Clements, Ernest J. Freeman, Christopher Malcuit, Jennifer A. McDonough, LaShanda T. J. Korley, Torsten Hegmann and Elda Hegmann

      Article first published online: 10 OCT 2014 | DOI: 10.1002/mabi.201400325

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      A new trick for LC elastomers: Biocompatible, biodegradable and porous liquid crystal (LC) elastomers were used for the first time as viable, soft scaffolds for cell growth and proliferation in a spatial (3D) fashion.

    5. Microcellular Open Porous Monoliths for Cell Growth by Thiol-Ene Polymerization of Low-Toxicity Monomers in High Internal Phase Emulsions

      Maja Sušec, Robert Liska, Günter Russmüller, Jiři Kotek and Peter Krajnc

      Article first published online: 8 OCT 2014 | DOI: 10.1002/mabi.201400219

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      Combination of high internal phase emulsion templating and thiol-ene polymerization of vinyl esters and thiols produced highly porous polymers with an interconnected morphology and tunable pore size. Usefulness of novel material is demonstrated by application as a scaffold for in vitro osteoblasts growth.

  6. Reviews

    1. Tissue-Engineered Cartilage: The Crossroads of Biomaterials, Cells and Stimulating Factors

      Nandana Bhardwaj, Dipali Devi and Biman B. Mandal

      Article first published online: 3 OCT 2014 | DOI: 10.1002/mabi.201400335

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      Over the years, cartilage tissue engineering is growing by leaps and bounds. Not only are various biomaterials being opted for repair, but newer methodologies being invented. With an aim to present an overview of current progress, we have summed up the advances and problems in cartilage regeneration with strong emphasis on material types, cells, stimulating factors and culture conditions.

  7. Full Papers

    1. Adsorption of Fibronectin on Salt-Etched Polyelectrolyte Multilayers and its Roles in Mediating the Adhesion and Migration of Vascular Smooth Muscle Cells

      Jun Deng, Mingcong Sun, Shasha Wang, Lulu Han, Zhengwei Mao, Dan Li, Hong Chen and Changyou Gao

      Article first published online: 3 OCT 2014 | DOI: 10.1002/mabi.201400344

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      The adsorption of fibronectin (Fn) and the adhesion and migration of vascular smooth muscle cells (VSMCs) are characterized and correlated on salt-treated PEI(PSS/PDDA)7 multilayers. The swelling properties and surface charge of the salt-treated multilayers jointly govern the adsorption manner, conformation, and activity of Fn, which in turn contribute heavily to the adhesion, spreading, and migration of VSMCs.

    2. New Semi-Biodegradable Materials from Semi-Interpenetrated Networks of Poly(ϵ-caprolactone) and Poly(ethyl acrylate)

      P. Lozano Picazo, M. Pérez Garnes, C. Martínez Ramos, A. Vallés-Lluch and M. Monleón Pradas

      Article first published online: 30 SEP 2014 | DOI: 10.1002/mabi.201400331

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      Semi-degradable materials may have find applications. Poly-(ethyl acrylate) and poly(ϵ-caprolactone) are here combined as semi-IPNs. Materials are obatined which combine their outstanding biological properties and possess mechanical properties tunable in a wide range of moduli. The behavior of fibroblasts and adipose-tissue-derived stem cells on these new materials is studied.

    3. Revisiting Complexation between DNA and Polyethylenimine: Does the Disulfide Linkage Play a Critical Role in Promoting Gene Delivery?

      Yongzheng Ma, Baizhu Chen, Ning He, Gaojian Chen, Lianwei Li and Chi Wu

      Article first published online: 29 SEP 2014 | DOI: 10.1002/mabi.201400253

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      To improve transfection efficiency and lower cytotoxicity, disulfide bonds have been applied in gene vectors. However, the disulfide bonds may not play an important role in promoting gene delivery, but do extend the chain length. A long free PEI chain could efficiently protect DNA from degradation, so that more DNA is transported into the nucleus.

    4. A Cancer-Recognizing Polymeric Photosensitizer Based on the Tumor Extracellular pH Response of Conjugated Polymers for Targeted Cancer Photodynamic Therapy

      Songhee Jeong, Wooram Park, Chung-Sung Lee and Kun Na

      Article first published online: 24 SEP 2014 | DOI: 10.1002/mabi.201400361

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      A cancer-recognizing polymeric photosensitizer (CRPP) for targeted photodynamic cancer therapy is developed through a facile synthetic process. The newly synthesized CRPP is capable of reversing its charge from negative to positive at the tumor extracellular pH (pHe, pH ∼6.5) to facilitate cellular internalization, which can lead to enhanced therapeutic efficacy against tumor cells.

    5. Sulfated Hyaluronan Influences the Formation of Artificial Extracellular Matrices and the Adhesion of Osteogenic Cells

      Alina Miron, Sandra Rother, Linda Huebner, Ute Hempel, Iris Käppler, Stephanie Moeller, Matthias Schnabelrauch, Dieter Scharnweber and Vera Hintze

      Article first published online: 12 SEP 2014 | DOI: 10.1002/mabi.201400292

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      The presence of sulfated glycosaminoglycans (GAG), like sulfated hyaluronan derivatives and chondroitin sulfate, during in vitro-fibrillogenesis of collagen type I alters the structure of the resulting artificial extracellular matrices. Besides, the GAG amount associated to collagen increases in a sulfation-dependent manner. Moreover, GAG sulfation influences the cytoskeletal arrangement of SaOS-2 cells which implies that adhesion-related intracellular signaling is affected.

    6. pH-Responsive Single Walled Carbon Nanotube Dispersion for Target Specific Release of Doxorubicin to Cancer Cells

      Moumita Ghosh, Sayanti Brahmachari and Prasanta Kumar Das

      Article first published online: 11 SEP 2014 | DOI: 10.1002/mabi.201400290

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      pH Responsive cholesterol based dipeptide carboxylates were synthesized as efficient dispersing agents for SWNTs in water. These highly dispersed nanotubes were used for the uploading of anticancer drug doxorubicin. The nanohybrid showed excellent pH responsive drug release at the extracellular tumorogenic environmental pH (6.0–6.5) and selectively kills cancer cells.

    7. Liposomal Drug Deposits in Poly(Dopamine) Coatings: Effect of Their Composition, Cell Type, Uptake Pathway Considerations, and Shear Stress

      Martin E. Lynge, Marina Fernandez-Medina, Almar Postma and Brigitte Städler

      Article first published online: 11 SEP 2014 | DOI: 10.1002/mabi.201400350

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      Different types of liposome-containing poly(dopamine)-based films were assembled and their interaction with adhering cells was assessed. The internalization of cargo was not affected by the presence of lipophilic dopamine conjugates in the lipid membrane but by the charge of the liposomes. Clathrin-mediated endocytosis was identified as uptake pathway. Shear stress had an impact on the interaction of adhering endothelial cells with the composite films.

    8. Sprayed Cells and Polyelectrolyte Films for Biomaterial Functionalization: The Influence of Physical PLL–PGA Film Treatments on Dental Pulp Cell Behavior

      Ivan V. Panayotov, Pierre-Yves Collart-Dutilleul, Hamideh Salehi, Marta Martin, Attila Végh, Jacques Yachouh, Boyan Vladimirov, Péter Sipos, Balázs Szalontai, Csilla Gergely and Frédéric J. G. Cuisinier

      Article first published online: 11 SEP 2014 | DOI: 10.1002/mabi.201400256

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      A good manufacturing practice (GMP)-compliant spraying method is described for the buildup of polyelectro-lyte multilayer (PEM) films constructed by poly-L-lysine (PLL) and poly-glutamic acid (PGA), onto which dental pulp cells are also deposited by spraying. The prepared PEM films were exposed to UV irradiation and drying/re-hydrating cycle. This procedure renders the films thicker, rougher and stiffer, enhancing dental pulp cell adhesion and proliferation.

    9. Bamboo Leaf-Like Micro-Nano Sheets Self-Assembled by Block Copolymers as Wafers for Cells

      Wen Zhu, Bo Peng, Jing Wang, Ke Zhang, Lixin Liu and Yongming Chen

      Article first published online: 10 SEP 2014 | DOI: 10.1002/mabi.201400283

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      Bamboo leaf-like micro-nano sheets are prepared via crystallization-driven self-assembly of poly(ethylene oxide)-b-poly(ϵ-caprolactone). The preparation is quite convenient within 1 h. These polymer sheets look like wafers for cells, and they do display a selective internalization to different cells. The macrophages show great interest in the wafers.

    10. Cell Motility on Polyethylene Glycol Block Copolymers Correlates to Fibronectin Surface Adsorption

      Peter J. F. Röttgermann, Samira Hertrich, Ida Berts, Max Albert, Felix J. Segerer, Jean-François Moulin, Bert Nickel and Joachim O. Rädler

      Article first published online: 10 SEP 2014 | DOI: 10.1002/mabi.201400246

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      Cell morphology and motility on artificial surfaces can be controlled by different degrees of surface PEGylations. Here, fibronectin incubation in three different PEG block copolymers is analyzed via neutron reflectivity. Fibronectin adsorbs in the vicinity of the PLL layer. The amount of fibronectin in the polymer layer is correlated to cell spreading and motility.

    11. Decomposition and Transformation of Pyrene-Derivative Micelles at Intracellular Milieu and Their Influence on Cytoviability

      Haisheng Wang, Wei Yu, Wenbo Zhang and Changyou Gao

      Article first published online: 10 SEP 2014 | DOI: 10.1002/mabi.201400338

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      Amphiphilic pyrene-containing random copolymers with pH-sensitive hydrazone bonds are synthesized. Spherical hydrolysable micelles formed by the amphiphilic copolymers at pH 6 are transformed into nanorods due to cleavage of the hydrazone bonds. The shape transformation is observed in pH 5.5 PBS too. The hydrolysable micelles cause higher toxicity to A549 cells than the non-hydrolysable ones without shape transformation.

    12. Preparation and Bioimaging Applications of AIE Dye Cross-linked Luminescent Polymeric Nanoparticles

      Meiying Liu, Xiqi Zhang, Bin Yang, Fengjie Deng, Yang Yang, Zhen Li, Xiaoyong Zhang and Yen Wei

      Article first published online: 8 SEP 2014 | DOI: 10.1002/mabi.201400262

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      We demonstrate that red luminescent polymeric nanoparticles can be prepared via combination of free radical polymerization and a ring-opening reaction using aggregation-induced emission dye as cross-linker. The good water dispersibility, size, enhanced fluorescence intensity, and excellent biocompatibility that these luminescent polymeric nanoparticles exhibit gives them good potential for biological imaging applications.

  8. Communications

    1. Collagen Surfaces Modified with Photo-Cleavable Polyethylene Glycol-Lipid Support Versatile Single-Cell Arrays of Both Non-adherent and Adherent Cells

      Shinya Yamahira, Satoshi Yamaguchi, Masahiro Kawahara and Teruyuki Nagamune

      Article first published online: 5 SEP 2014 | DOI: 10.1002/mabi.201400312

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      A smart-material surface for simply and rapidly preparing versatile single-cell arrays of both non-adherent and adherent cells was designed and constructed based on a photo-cleavable polyethylene glycol (PEG)-lipid. Two high-throughput image analysis applications were demonstrated on these PEG-lipid-based single cell arrays. In addition, extremely densely-packed single-cell arrays were successfully constructed.

  9. Full Papers

    1. In Vitro Synergistic Action of Geldanamycin- and Docetaxel-Containing HPMA Copolymer–RGDfK Conjugates Against Ovarian Cancer

      Nate Larson, Sarah Roberts, Abhijit Ray, Brandon Buckway, Darwin L. Cheney and Hamidreza Ghandehari

      Article first published online: 4 SEP 2014 | DOI: 10.1002/mabi.201400360

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      In vitro evaluation of combination therapy utilizing HPMA copolymer–RGDfK conjugates. Aminohexylgeldanamycin conjugates are more stable and exhibit slower drug release than docetaxel conjugates. Both conjugates demonstrate binding specifically to αvβ3 integrins. HPMA copolymer–RGDfK conjugates bearing aminohexylgeldanamycin and docetaxel induce cytotoxicity synergistically suggesting potential as combination therapy for ovarian cancer.

  10. Communications

    1. Microfluidic Synthesis of Pharmacologically Responsive Supramolecular Biohybrid Microgels

      Désirée Hövermann, Torsten Rossow, Raphael J. Gübeli, Sebastian Seiffert and Wilfried Weber

      Article first published online: 3 SEP 2014 | DOI: 10.1002/mabi.201400342

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      We describe the microfluidic synthesis of pharmacologically responsive biohybrid hydrogel particles synthesized from 8-arm poly(ethylene glycol) coupled to fluorescein and a fluorescein-specific humanized antibody fragment. Mixing the reactants in a microfluidic device results in narrowly dispersed microgels that can be degraded selectively by addition of fluorescein to disrupt the antigen–antibody ties.

  11. Reviews

    1. You have free access to this content
      Polymeric Anti-HIV Therapeutics

      Maarten Danial and Harm-Anton Klok

      Article first published online: 3 SEP 2014 | DOI: 10.1002/mabi.201400298

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      Anti-HIV therapeutics have enabled to improve the lives of many infected individuals and shifted the prognosis to a chronic but manageable disease. In parallel to the development of small molecule drug therapeutics, profound interest and efforts have been made to develop polymeric anti-HIV therapeutics. An overview of these macromolecular therapeutics is presented herein.

  12. Full Papers

    1. A Combined Micelle and Poly(Serinol Hexamethylene Urea)-Co-Poly(N-Isopropylacrylamide) Reverse Thermal Gel as an Injectable Ocular Drug Delivery System

      Amin Famili, Malik Y. Kahook and Daewon Park

      Article first published online: 3 SEP 2014 | DOI: 10.1002/mabi.201400250

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      A poorly-soluble ocular corticosteroid is encapsulated within polymeric micelles, which are mixed with a novel reverse thermal gel to form an injectable long-term drug delivery system. This combined system improves release kinetics of the drug over micelles or reverse thermal gel alone by eliminating burst release behavior and extending the timeframe of drug release.

    2. Structurally Distinct Hybrid Polymer/Lipid Nanoconstructs Harboring a Type-I Ribotoxin as Cellular Imaging and Glioblastoma-Directed Therapeutic Vectors

      M. Sheikh Mohamed, Srivani Veeranarayanan, Ankur Baliyan, Aby Cheruvathoor Poulose, Yutaka Nagaoka, Hiroaki Minegishi, Seiki Iwai, Yasuhiro Shimane, Yasuhiko Yoshida, Toru Maekawa and D. Sakthi Kumar

      Article first published online: 2 SEP 2014 | DOI: 10.1002/mabi.201400248

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      A multicomponent polymeric/lipid nano-construct encompassing a plant toxin and imaging dye was utilized to specifically curb the proliferation tendencies of glioblastoma in addition to suppressing the anti-apoptotic survivin and membrane interaction vinculin proteins. The cytocompatibility and multifunctionality of the nanoparticles significantly projects their prospective theranostic potential.

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