In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels
Matilde Bongio, M. Reza Nejadnik, Z. Tahmasebi Birgani, Pamela Habibovic, Lucas A. Kinard, F. Kurtis Kasper, Antonios G. Mikos, John A. Jansen, Sander C. G. Leeuwenburgh and Jeroen J. J. P. van den Beucken
Article first published online: 10 APR 2013 | DOI: 10.1002/mabi.201200474
Hydrogels are highly hydrated polymers with structural properties similar to soft tissues. In view of bone regenerative applications, the incorporation of alkaline phosphatase (ALP) within hydrogels is a simple and promising strategy to generate scaffolds with both an organic and inorganic phase. Specifically, the enzymatic hydrolysis of organic phosphates, diffused from the physiological environment, leads to the formation of minerals.