Macromolecular Bioscience

Cover image for Vol. 15 Issue 11

Early View (Online Version of Record published before inclusion in an issue)

Editor-in-Chief: Kirsten Severing, Editor: Anne Pfisterer

Online ISSN: 1616-5195

Associated Title(s): Advanced Healthcare Materials, Macromolecular Chemistry and Physics, Macromolecular Materials and Engineering, Macromolecular Rapid Communications


  1. 1 - 41
  1. Communications

    1. Cellularized Cellular Solids via Freeze-Casting

      Sarah Christoph, Julien Kwiatoszynski, Thibaud Coradin and Francisco M. Fernandes

      Article first published online: 4 NOV 2015 | DOI: 10.1002/mabi.201500319

      Thumbnail image of graphical abstract

      The elaboration of metabolically active cell-containing materials is a decisive step toward the successful application of cell based technologies. The present work unveils a new process allowing to simultaneously encapsulate living cells and shaping cell-containing materials into solid-state macroporous foams with precisely controlled morphology using freeze casting.

  2. Full Papers

    1. Patterned Polypeptoid Brushes

      Maximilian Schneider, Zian Tang, Marcus Richter, Claudia Marschelke, Paul Förster, Erik Wegener, Ihsan Amin, Heike Zimmermann, Dieter Scharnweber, Hans-Georg Braun, Robert Luxenhofer and Rainer Jordan

      Article first published online: 2 NOV 2015 | DOI: 10.1002/mabi.201500314

      Thumbnail image of graphical abstract

      The preparation of patterned polypeptoid (POI) brushes on gold and oxide substrates by surface-initiated polymerization of N-substituted glycine N-carboxyanhydrides from patterned self-assembled monolayers is presented. Furthermore, the chemoselective functionalization and anti-biofouling properties of POI brushes are shown.

    2. A Stimulated Mixed Micelle System for In Vitro Study on Chemo-Photodynamic Therapy

      Ching-Yi Chen, Chao-Kai Syu and Hsin Chang Lin

      Article first published online: 8 OCT 2015 | DOI: 10.1002/mabi.201500269

      Thumbnail image of graphical abstract

      A stimulated mixed micelle system with photosensitizer-centered for encapsulation of doxorubicin was developed for chemo-photodynamic therapy. This mixed micelle system shows good stability, controlled release rate and high singlet oxygen generation efficiency. In vitro study demonstrates a higher cytotoxicity under light condition relative to individual single drug loaded micelle, indicating improved therapeutic efficiency due to combination therapy.

    3. PolyDOPA Mussel-Inspired Coating as a Means for Hydroxyapatite Entrapment on Polytetrafluoroethylene Surface for Application in Periodontal Diseases

      Tiziana Nardo, Valeria Chiono, Gianluca Ciardelli and Maryam Tabrizian

      Article first published online: 7 OCT 2015 | DOI: 10.1002/mabi.201500241

      Thumbnail image of graphical abstract

      This work gives a thorough characterization of non-resorbable polytetrafluoroethylene films, coated with hydroxyapatite nanoparticles (HAp) through an adhesive coating of self-polymerized 3,4-dihydroxy-DL-phenylalanine (polyDOPA). This coating technique represents an interesting approach able to improve MC3T3-E1 pre-osteoblastic cells adhesion and proliferation on polyDOPA/HAp-coated substrates.

    4. Non-Fouling Biodegradable Poly(ϵ-caprolactone) Nanofibers for Tissue Engineering

      Nina Yu. Kostina, Ognen Pop-Georgievski, Michael Bachmann, Neda Neykova, Michael Bruns, Jiří Michálek, Martin Bastmeyer and Cesar Rodriguez-Emmenegger

      Article first published online: 7 OCT 2015 | DOI: 10.1002/mabi.201500252

      Thumbnail image of graphical abstract

      Four antifouling polymer brushes are grown from biodegradable poly(ϵ-caprolactone) nanofibers without impairing their unique architecture. The nanofibers are challenged with mouse embryonic fibroblasts. Fibroblasts are repelled from the nanofibers coated with brushes while the cells could rapidly adhere and form cell–matrix adhesions on pristine nanofibers. The nanofibers modified with protein repellent brushes are able to suppress cell adhesion.

    5. A Periosteum-Inspired 3D Hydrogel-Bioceramic Composite for Enhanced Bone Regeneration

      Yong Yao Chun, Jun Kit Wang, Nguan Soon Tan, Peggy Puk Yik Chan, Timothy Thatt Yang Tan and Cleo Choong

      Article first published online: 7 OCT 2015 | DOI: 10.1002/mabi.201500258

      Thumbnail image of graphical abstract

      A hydrogel-bioceramic composite consisting of fish scale-derived CaP incorporated into a Gtn-HPA/CMC-Tyr hydrogel is demonstrated as a potential periosteum-inspired injectable system to enhance bone healing. The incorporation of CaP into the hydrogel significantly improves its mechanical properties, provides a bioactive environment for nucleation and formation of apatite, encourages cell proliferation, and shows a zero order FITC-dextran release profile.

    6. Disruption of Amyloid Prion Protein Aggregates by Cationic Pyridylphenylene Dendrimers

      Svetlana A. Sorokina, Yulia Yu. Stroylova, Zinaida B. Shifrina and Vladimir I. Muronetz

      Article first published online: 7 OCT 2015 | DOI: 10.1002/mabi.201500268

      Thumbnail image of graphical abstract

      Cationic pyridylphenylene dendrimers are able to disrupt amyloid protein aggregates, e.g., prion protein inclusion bodies (PrP IB). The incubation of PrP IB with the dendrimers leads to formation of stable PrP-dendrimer complexes and the amyloid properties of PrP are decreased upon the interaction with the dendrimer.

  3. Communications

    1. Acidity-Induced Destabilization of Nano-Sized Supramolecular Linear-Hyperbranched Polymersome for Controlled Release of Encapsulated Cargoes

      Xing Dong, Bin Yang, Hui-zhen Jia, Jing-yi Zhu, Ren-xi Zhuo, Jun Feng and Xian-zheng Zhang

      Article first published online: 7 OCT 2015 | DOI: 10.1002/mabi.201500298

      Thumbnail image of graphical abstract

      A linear-hyperbranched supramolecular amphiphile is designed. The host-guest interaction in the amphiphilic construction allows not only control over assembly types (solid and hollow nanoparticles), but also the one-step accomplishment of both polymersome fabrication and drug encapsulation. The vesicles can release the encapsulated hydrophilic agents in an acidity-accelerated manner.

  4. Full Papers

    1. Tailored Fringed Platforms Produced by Laser Interference for Aligned Neural Cell Growth

      Ramón J. Peláez, Ankor González-Mayorga, María C. Gutiérrez, Concepción García-Rama, Carmen N. Afonso and María C. Serrano

      Article first published online: 6 OCT 2015 | DOI: 10.1002/mabi.201500253

      Thumbnail image of graphical abstract

      Laser interferometry is as a versatile and easy-to-use technique to fabricate fringed patterns on thin partially reduced graphene oxide layers with interest for cell alignment. Neural cells grown on these platforms significantly align their neurites along the direction of the fringes and preserve high viability in culture for up to 2 weeks.

    2. Stiffness of Hydrogels Regulates Cellular Reprogramming Efficiency Through Mesenchymal-to-Epithelial Transition and Stemness Markers 

      Bogyu Choi, Kwang-Sook Park, Ji-Ho Kim, Kyoung-Won Ko, Jin-Su Kim, Dong Keun Han and Soo-Hong Lee

      Article first published online: 6 OCT 2015 | DOI: 10.1002/mabi.201500273

      Thumbnail image of graphical abstract

      The stiffness of hyd rogels can be used as a potent regulator to promote cell fate changes associated with reprogramming into induced pluripotent stem cells (iPSCs) via increased mesenchymal-to-epithelial transition activity and stem cell marker expression. This study can lead to effective and reproducible iPSC production and be applied to iPSC-based therapy.

    3. Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X

      Kerstin Uhde-Holzem, Michael McBurney, Brylee David B. Tiu, Rigoberto C. Advincula, Rainer Fischer, Ulrich Commandeur and Nicole F. Steinmetz

      Article first published online: 6 OCT 2015 | DOI: 10.1002/mabi.201500280

      Thumbnail image of graphical abstract

      The in planta nanomanufacturing of immunosorbent, high aspect ratio nanoparticles for orientationally controlled antibody display is reported. The authors produce potato virus X-based nanofilaments with genetically introduced protein A fragments and demonstrate antibody capture for biosensing applications.

    4. Thiol-ene Photocrosslinking of Cytocompatible Resilin-Like Polypeptide-PEG Hydrogels

      Christopher L. McGann, Rebekah E. Dumm, Anna K. Jurusik, Ishnoor Sidhu and Kristi L. Kiick

      Article first published online: 5 OCT 2015 | DOI: 10.1002/mabi.201500305

      Thumbnail image of graphical abstract

      Efficient photoinitiated crosslinking via thiol-ene step polymerization is conferred to hybrid hydrogels composed of poly(ethylene glycol) star macromers and a recombinant elastomeric biopolymer based upon resilin, an insect protein. The hydrogels form elastic polymer networks and demonstrate cytocompatibility with human mesenchymal stem cells making them potential candidates as tissue engineering scaffolds for mechanically demanding applications.

    5. Phenolic Acid-based Poly(anhydride-esters) as Antioxidant Biomaterials

      Almudena Prudencio, Jonathan J. Faig, MinJung Song and Kathryn E. Uhrich

      Article first published online: 1 OCT 2015 | DOI: 10.1002/mabi.201500244

      Thumbnail image of graphical abstract

      Naturally occurring, antioxidant-based poly(anhydride-esters) are synthesized in high yields via solution polymerization methods. Structures of precursor and polymer are determined and verified by NMR and Fourier transform IR spectroscopies. The polymers exhibit sustained hydrolytic release of phenolic acids over the 30 day study, maintaining antioxidant potency when compared to free bioactives.

    6. Layer-by-Layers of Polymeric Micelles as a Biomimetic Drug-Releasing Network

      Sundar P. Authimoolam, Andrew L. Lakes, David A. Puleo and Thomas D. Dziubla

      Article first published online: 29 SEP 2015 | DOI: 10.1002/mabi.201500310

      Thumbnail image of graphical abstract

      Filomicelle networks possess high biomimetic relevance to natural mucin. In this work, these synthetic networks are evaluated for its ability to function as drug-releasing network. In developing a tunable release, dense barrier is deposited atop networks, and critically evaluated for its drug release and chemical stability. These tunable networks can potentially serve as a functional coat rendering localized delivery.

    7. Synthesis and Enzymatic Degradation of Soft Aliphatic Polyesters

      Viola Buchholz, Seema Agarwal and Andreas Greiner

      Article first published online: 24 SEP 2015 | DOI: 10.1002/mabi.201500279

      Thumbnail image of graphical abstract

      Synthesis of novel aliphatic enzymatically degradable polyesters with short side chains leads to variable features. The influence of the reduced crystallinity on thermal and mechanical properties, melting behavior and Young's modulus, is reported. The controllable degradation behavior in combination with the reduced brittleness offers new possible applications for aliphatic polyesters.

    8. A Novel Small-Caliber Bacterial Cellulose Vascular Prosthesis: Production, Characterization, and Preliminary In Vivo Testing

      Alexandre F. Leitão, Miguel A. Faria, Augusto M. R. Faustino, Ricardo Moreira, Petra Mela, Luís Loureiro, Ivone Silva and Miguel Gama

      Article first published online: 21 SEP 2015 | DOI: 10.1002/mabi.201500251

      Thumbnail image of graphical abstract

      Bacterial cellulose (BC) is among the polymers being studied for use as an off-the-shelf vascular graft. The authors present a novel, facile, and cost-effective method for small caliber BC grafts production. The morphology of the graft wall, tensile strength, and luminal topography all show promising results. The in vivo behavior of these BC grafts further demonstrate their viability achieving, one month patency with neo-vessels, and endothelial cells presenting on the luminal surface of the graft.

    9. Chemoenzymatic Synthesis of Oligo(l-cysteine) for Use as a Thermostable Bio-Based Material

      Yinan Ma, Ryota Sato, Zhibo Li and Keiji Numata

      Article first published online: 21 SEP 2015 | DOI: 10.1002/mabi.201500255

      Thumbnail image of graphical abstract

      Oligomerization of thiol-unprotected l-cysteine ethyl ester catalyzed by proteinase K in aqueous solution has been used to synthesize oligo(l-cysteine) with high free thiol content and relative large degree of polymerization. The products exhibit stable thermal properties and have great potential for use as a thermostable bio-based material with resistance to oxidation.

    10. Synthesis of Amphiphilic Hyperbranched AIE-active Fluorescent Organic Nanoparticles and Their Application in Biological Application

      Qiulan Lv, Ke Wang, Dazhuang Xu, Meiying Liu, Qing Wan, Hongye Huang, Shangdong Liang, Xiaoyong Zhang and Yen Wei

      Article first published online: 16 SEP 2015 | DOI: 10.1002/mabi.201500256

      Thumbnail image of graphical abstract

      A facile and effective strategy has been reported for fabrication of AIE-active nanoprobes trough one-step Michael addition reaction.

  5. Communications

    1. Chemical and Enzymatic Site Specific PEGylation of hGH: The Stability and in vivo Activity of PEG-N-Terminal-hGH and PEG-Gln141-hGH Conjugates

      Antonella Grigoletto, Anna Mero, Ilenia Zanusso, Oddone Schiavon and Gianfranco Pasut

      Article first published online: 9 SEP 2015 | DOI: 10.1002/mabi.201500282

      Thumbnail image of graphical abstract

      Two different site-specific monoPEGylated forms of hGH are prepared using a chemical, N-terminal PEGylation and enzymatic, transglutaminase-based conjugation approaches. The N-terminal PEGylation yielded a conjugate with a higher thermal stability and, of particular interest, one that is able to recover the secondary structure after thermal denaturation.

    2. Mold-Based Application of Laser-Induced Periodic Surface Structures (LIPSS) on Biomaterials for Nanoscale Patterning

      Wim Hendrikson, Wendy Masman-Bakker, Bas van Bochove, Johann Skolski, Justus Eichstädt, Bart Koopman, Clemens van Blitterswijk, Dirk Grijpma, Gert-willem Römer, Lorenzo Moroni and Jeroen Rouwkema

      Article first published online: 3 SEP 2015 | DOI: 10.1002/mabi.201500270

      Thumbnail image of graphical abstract

      Laser-induced periodic surface structures (LIPSS) are applied to poly(trimethylene carbonate) (PTMC) and poly(ethylene glycol)–poly(trimethylene carbonate) (PEG–PTMC) polymeric biomaterial surfaces using a mold-based approach. This results in nanoscale surface features that are regular, but at the same time contain a certain level of disorder which is beneficial for cellular responses.

  6. Full Papers

    1. Regional Morphology and Transport of PAMAM Dendrimers Across Isolated Rat Intestinal Tissue

      Dallin Hubbard, Tanner Bond and Hamidreza Ghandehari

      Article first published online: 2 SEP 2015 | DOI: 10.1002/mabi.201500225

      Thumbnail image of graphical abstract

      PAMAM dendrimers have been observed to traverse the intestinal epithelium as a macromolecule. Herein is established the regional dependence of PAMAM dendrimers in isolated tissue models using rat intestinal epithelium. The histology of isolated rat intestinal tissues in the presence of PAMAM dendrimers is established.

    2. Investigation of Antifouling Properties of Surfaces Featuring Zwitterionic α-Aminophosphonic Acid Moieties

      Natalie Wagner, Phyllis Zimmermann, Peter Heisig, Franziska Klitsche, Wolfgang Maison and Patrick Theato

      Article first published online: 2 SEP 2015 | DOI: 10.1002/mabi.201500196

      Thumbnail image of graphical abstract

      Zwitterionic α-aminophosphonic acid surfaces are prepared by successful surface-Kabachnik–Fields reaction of an aldehyde-functionalized surface with amines and dialkyl phosphonates and subsequent deprotection. The obtained zwitterionic surfaces exhibit antifouling properties.

    3. Protein-Containing Multilayer Capsules by Templating on Mesoporous CaCO3 Particles: POST- and PRE-Loading Approaches

      Nadezhda G. Balabushevich, Anna V. Lopez de Guerenu, Natalia A. Feoktistova, Andre G. Skirtach and Dmitry Volodkin

      Article first published online: 26 AUG 2015 | DOI: 10.1002/mabi.201500243

      Thumbnail image of graphical abstract

      Protein encapsulation into polymer-based multilayer capsules is performed by templating on sacrificial porous CaCO3 microparticles. We focus on protein encapsulation by considering two approaches: POST-loading into performed capsules and PRE-loading into the carbonate microparticles by adsorption or co-synthesis. Protein content and its activity strongly depend on the encapsulation approach to be chosen for successful encapsulation.

    4. Highly Fluorescent Gene Carrier Based on Ag–Au Alloy Nanoclusters

      Ping Wang, Lin Lin, Zhaopei Guo, Jie Chen, Huayu Tian, Xuesi Chen and Hua Yang

      Article first published online: 19 AUG 2015 | DOI: 10.1002/mabi.201500235

      Thumbnail image of graphical abstract

      This research has successfully prepared a highly fluorescent gene carrier Ag–Au–PEI, which shows much higher quantum yield than most of the reported gold nanocluster-based quantum dots. The high fluorescent Ag–Au–PEI conjugates not only exhibit remarkable high gene transfection efficiencies and relatively low cytotoxicity in vitro but also possess bioimaging capacity in vivo.

    5. Enhanced Cytoplasmic Delivery of RAGE siRNA Using Bioreducible Polyethylenimine-based Nanocarriers for Myocardial Gene Therapy

      Min Jung Yang, Sook Hee Ku, Dongkyu Kim, Won Jong Kim, Hyejung Mok, Sun Hwa Kim and Ick Chan Kwon

      Article first published online: 19 AUG 2015 | DOI: 10.1002/mabi.201500213

      Thumbnail image of graphical abstract

      Bioreducible polyethylenimine (rPEI) is synthesized from low molecular weight PEI via cleavable disulfide linkages to achieve high stability, high transfection efficiency, high cytoplasmic siRNA release, and low cytotoxicity. rPEI/siRAGE polyplex can reduce RAGE expression in ischemic/reperfused myocardium, thereby leading to attenuation of myocardial infarction.

    6. Self-Assembly of a Multifunctional Lipid With Core–Shell Dendrimer DNA Nanoparticles Enhanced Efficient Gene Delivery at Low Charge Ratios into RPE Cells

      Da Sun, Hiroshi Maeno, Maneesh Gujrati, Rebecca Schur, Akiko Maeda, Tadao Maeda, Krzysztof Palczewski and Zheng-Rong Lu

      Article first published online: 13 AUG 2015 | DOI: 10.1002/mabi.201500192

      Thumbnail image of graphical abstract

      A hybrid gene delivery system comprised of a multifunctional lipid ECO and a core–shell dendrimer mediates high gene transfection efficiency at a low charge ratio in RPE cells. The non-viral delivery system is effective for in vivo gene transfection in the RPE cells after subretinal injection and promising in treating genetic disorders of the eye.

    7. Asymmetric AB3 Miktoarm Star Polymers: Synthesis, Self-Assembly, and Study of Micelle Stability Using AF4 for Efficient Drug Delivery

      Alexandre Moquin, Anjali Sharma, Yiming Cui, Anthony Lau, Dusica Maysinger and Ashok Kakkar

      Article first published online: 11 AUG 2015 | DOI: 10.1002/mabi.201500186

      Thumbnail image of graphical abstract

      AB3 (PCL–PEG3) miktoarm polymers form micelles and incorporate large amount of hydrophobic drug (curcumin). Size distribution of the micelles is narrow as determined by AF4 fractionation. Curcumin-loaded AB3 micelles exert significant cell loss in glioblastoma (GBM) spheroids.

    8. Long-Term Controlled Protein Release from Poly(Ethylene Glycol) Hydrogels by Modulating Mesh Size and Degradation

      Xinming Tong, Soah Lee, Layla Bararpour and Fan Yang

      Article first published online: 11 AUG 2015 | DOI: 10.1002/mabi.201500245

      Thumbnail image of graphical abstract

      A PEG-based hydrogel platform is developed to allow long-term controlled protein release. No protein is released from non-degradable PEG hydrogels, confirming efficient loading via physical entrapment. Degradation of hydrogels increasing swelling ratio and mesh size enables sustained protein release. bFGF released from hydrogel over 35 d continues to stimulate cell proliferation comparable to fresh bFGF.

    9. Efficient pDNA Delivery Using Cationic 2-Hydroxypropyl-β-Cyclodextrin Pluronic-Based Polyrotaxanes

      Vivek Badwaik, Yawo Mondjinou, Aditya Kulkarni, Linjia Liu, Asher Demoret and David H. Thompson

      Article first published online: 10 AUG 2015 | DOI: 10.1002/mabi.201500220

      Thumbnail image of graphical abstract

      Dried α, ω-bis-tris (2-aminoethyl) amine Pluronic triblock copolymer (0.08 mmol) and 2-hydroxypropyl-β-cyclodextrin (using the ratio of CD:PPG unit = 1:2 for each type of Pluronic) were dissolved (or suspended) in 60 mL hexane and the mixtures were vortexed before vigorously stirring for 2 h. The mixtures were then gently stirred for 72 h at 20 °C and cholesteryl chloroformate (12 equiv) was used to cap the polyrotaxanes before purification and lyophilization.

    10. Cellular Response to Linear and Branched Poly(acrylic acid)

      Elizabeth G. Whitty, Alison R. Maniego, Sharon A. Bentwitch, Yohann Guillaneuf, Mark R. Jones, Marianne Gaborieau and Patrice Castignolles

      Article first published online: 10 AUG 2015 | DOI: 10.1002/mabi.201500153

      Thumbnail image of graphical abstract

      The cytotoxicity and intracellular effects of poly(acrylic acid)/poly(sodium acrylate) (PNaA) are investigated. This pH-responsive polymer has potential in anticancer drug delivery. Capillary electrophoresis demonstrates interactions of PNaA with serum proteins. The MTT assay is used to assess the cytotoxicity of PNaA with different branching architectures for L1210 progenitor leukemia cells and L6 myoblast cells.

    11. Nontoxic, Hydrophilic Cationic Polymers—Identified as Class of Antimicrobial Polymers

      Arne Strassburg, Frauke Kracke, Julia Wenners, Anna Jemeljanova, Jannis Kuepper, Hanne Petersen and Joerg C. Tiller

      Article first published online: 4 AUG 2015 | DOI: 10.1002/mabi.201500207

      Thumbnail image of graphical abstract

      Antimicrobially active and nontoxic hydrophilic polycations based on 1,4-dibromo-2-butene and tetramethyl-1,3-propanediamine named PBI are an alternative to toxic biocides and amphiphilic polycations. Investigations on PBIs with defined molecular weight (Mn) and tailored end groups revealed that there is a dependence of antimicrobial activity and selectivity on Mn and nature of the end groups.

  7. Communications

    1. Star-Shaped, Biodegradable, and Elastomeric PLLA-PEG-POSS Hybrid Membrane With Biomineralization Activity for Guiding Bone Tissue Regeneration

      Meihua Xie, Juan Ge, Bo Lei, Qian Zhang, Xiaofeng Chen and Peter X. Ma

      Article first published online: 4 AUG 2015 | DOI: 10.1002/mabi.201500237

      Thumbnail image of graphical abstract

      Novel degradable star-shaped poly-L-lactide-poly (ethylene glycol)-polyhedral oligomeric silsesquioxane (SPPS) hybrid implants with highly controlled mechanical properties, and with elastomeric behavior, biomineralization activity, and cell biocompatibility are fabricated successfully by a facile photo-crosslinking method.

  8. Feature Articles

    1. Biofunctional Materials Based on Amino Cellulose Derivatives – A Nanobiotechnological Concept

      Thomas Heinze, Melanie Siebert, Peter Berlin and Andreas Koschella

      Article first published online: 30 JUL 2015 | DOI: 10.1002/mabi.201500184

      Thumbnail image of graphical abstract

      Amino cellulose derivatives simply available by nucleophilic displacement reactions of tosyl cellulose with a broad variety of amines possess an extraordinary ability for self-assembling. Nanocomposites and monolayers may be formed on various substrates. For example, the modified surfaces are efficient carriers for (bio) functional molecules yielding efficient biosensors.

  9. Communications

    1. Anti-Resorptive Functions of Poly(ethylene sodium phosphate) on Human Osteoclasts

      Sujit Kootala, Masahiro Tokunaga, Jöns Hilborn and Yasuhiko Iwasaki

      Article first published online: 29 JUL 2015 | DOI: 10.1002/mabi.201500166

      Thumbnail image of graphical abstract

      The effect of poly(ethylene sodium phosphate) (PEP · Na) on human osteoclasts is studied. PEP · Na exhibits excellent biocompatibility with osteoblasts, whereas the polymer effectively reduces the adhesion of osteoclasts adhered on bone slices. This is the first report to describe the possibility of using polyphosphoesters for selective inhibition of osteoclast adhesion and function.

    2. Anti-Bioadhesive Coating Based on Easy to Make Pseudozwitterionic RAFT Block Copolymers for Blood-Contacting Applications

      Sabrina Nehache, Chin-Cheng Yeh, Mona Semsarilar, André Deratani, Yung Chang and Damien Quemener

      Article first published online: 29 JUL 2015 | DOI: 10.1002/mabi.201500185

      Thumbnail image of graphical abstract

      Well-defined pseudozwitterionic diblock copolymers are synthesized using RAFT chemistry. These charged polymers are then used to prepare anti-bioadhesion surfaces that could be used in blood-contact devices. It is demonstrated that such coatings prevent blood protein adsorption to the polymer modified surfaces.

  10. Full Papers

    1. Bioreducible Poly-l-Lysine–Poly[HPMA] Block Copolymers Obtained by RAFT-Polymerization as Efficient Polyplex-Transfection Reagents

      Kristof Tappertzhofen, Simone Beck, Evelyn Montermann, David Huesmann, Matthias Barz, Kaloian Koynov, Matthias Bros and Rudolf Zentel

      Article first published online: 29 JUL 2015 | DOI: 10.1002/mabi.201500212

      Thumbnail image of graphical abstract

      Polylysine-S-S-b-poly[N-(2-hydroxypropyl) methacrylamide] (p[HPMA]) copolymers with a bioreducible disulfide group can be obtained by RAFT polymerization of a macromolecular disulfide chain transfer agent (CTA). Polyplex formation with pDNA leads to increased transfection efficiencies compared to non disulfide containing block copolymers.

    2. N-(2-Hydroxypropyl)methacrylamide Copolymer–Drug Conjugates for Combination Chemotherapy of Acute Myeloid Leukemia

      Rui Zhang, Jiyuan Yang, Yan Zhou, Paul J. Shami and Jindřich Kopeček

      Article first published online: 29 JUL 2015 | DOI: 10.1002/mabi.201500193

      Thumbnail image of graphical abstract

      Four different drugs, including cytarabine, daunorubicin, GDC-0980, and JS-K, are used for two-drug combination treatment against acute myeloid leukemia. Combining cytarabine with GDC-0980 shows the strongest synergistism. N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-cytarabine/GDC-0980 conjugates are synthesized. They also hve potent cytotoxicity and strong synergstism in combination.

    3. Exploring the Properties of Genetically Engineered Silk-Elastin-Like Protein Films

      Raul Machado, André da Costa, Vitor Sencadas, Ana Margarida Pereira, Tony Collins, José Carlos Rodríguez-Cabello, Senentxu Lanceros-Méndez and Margarida Casal

      Article first published online: 27 JUL 2015 | DOI: 10.1002/mabi.201500132

      Thumbnail image of graphical abstract

      Free standing films of a genetically engineered silk-elastin-like protein are prepared by solvent casting using water and formic acid as solvents. The films are exposed to methanol-saturated air to induce the formation of aggregated β-strands and extensively characterized for their properties. Results indicate that the films are electrically insulating with excellent optical clarity and mechanical properties, depending of the post-treatment and glycerol content.

  11. Communications

    1. Orthotopic Osteogenecity Enhanced by a Porous Gelatin Sponge in a Critical-Sized Rat Calvaria Defect

      Naofumi Kanda, Takahisa Anada, Takuto Handa, Kazuhito Kobayashi, Yushi Ezoe, Tetsu Takahashi and Osamu Suzuki

      Article first published online: 22 JUL 2015 | DOI: 10.1002/mabi.201500191

      Thumbnail image of graphical abstract

      Preparation of gelatin disks for bone regeneration: this study is designed to investigate whether a gelatin sponge with a specific porous structure exhibits orthotopic osteogenecity in critical-sized rat calvarial defects. The results provide the findings that a Gel sponge is capable of inducing new bone formation orthotopically, if the scaffold has a specific porous structure.

  12. Full Papers

    1. Investigation of Functionalized Poly(N,N-dimethylacrylamide)-block-polystyrene Nanoparticles As Novel Drug Delivery System to Overcome the Blood–Brain Barrier In Vitro

      Maria Gregori, Daniela Bertani, Emanuela Cazzaniga, Antonina Orlando, Michele Mauri, Alberto Bianchi, Francesca Re, Silvia Sesana, Stefania Minniti, Maura Francolini, Alfredo Cagnotto, Mario Salmona, Luca Nardo, Domenico Salerno, Francesco Mantegazza, Massimo Masserini and Roberto Simonutti

      Article first published online: 21 JUL 2015 | DOI: 10.1002/mabi.201500172

      Thumbnail image of graphical abstract

      In the search of novel drug carriers for brain drug delivery, poly(N,N-dimethylacrylamide)-b-polystyrene nanoparticles (NP) have been functionalized with a modified fragment of Apolipoprotein E (mApoE). These NP show to be uptaken and to overcome a BBB in vitro cellular model. The cytotoxicity of NP is also investigated on different cell lines, showing high biocompatibility.

  13. Communications

    1. Microfluidic Production of Alginate Hydrogel Particles for Antibody Encapsulation and Release

      Linas Mazutis, Remigijus Vasiliauskas and David A. Weitz

      Article first published online: 21 JUL 2015 | DOI: 10.1002/mabi.201500226

      Thumbnail image of graphical abstract

      Using a microfluidic device with a characteristic flow-focusing junction that facilitates the break-up of viscous fluids into monodisperse droplets the authors produce alginate microparticles of unique biconcave shape resembling the red blood cells. They loaded alginate particles with fluorescently labeled antibodies and evaluated their release over time in different aqueous buffers and tested the biocompatibility in the whole blood sample.


  1. 1 - 41