Macromolecular Bioscience

Cover image for Vol. 16 Issue 2

Early View (Online Version of Record published before inclusion in an issue)

Editor-in-Chief: Kirsten Severing, Editor: Anne Pfisterer

Online ISSN: 1616-5195

Associated Title(s): Advanced Healthcare Materials, Macromolecular Chemistry and Physics, Macromolecular Materials and Engineering, Macromolecular Rapid Communications

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  1. 1 - 48
  1. Full Papers

    1. Design of a Novel Composite H2S-Releasing Hydrogel for Cardiac Tissue Repair

      Arianna Mauretti, Annalisa Neri, Olga Kossover, Dror Seliktar, Paolo Di Nardo and Sonia Melino

      Article first published online: 9 FEB 2016 | DOI: 10.1002/mabi.201500430

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      New composite 3D-hydrogel scaffolds with embedded protein-microbubbles are manufactured and assessed for their potential scaffolding properties for the growth of both fibroblasts and Sca1+ cardiac progenitor cells. Furthermore, enzymatic H2S-releasing microbubbles are produced and embedded in 3D scaffolds for tissue repair. The effects of this important gasotransmitter on the cell proliferation of human progenitor cells are studied using a novel 3D H2S-releasing scaffold.

    2. Polydopamine Inter-Fiber Networks: New Strategy for Producing Rigid, Sticky, 3D Fluffy Electrospun Fibrous Polycaprolactone Sponges

      Wuyong Choi, Slgirim Lee, Seung-Hyun Kim and Jae-Hyung Jang

      Article first published online: 8 FEB 2016 | DOI: 10.1002/mabi.201500375

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      Inter-molecular crosslinking polydopamine networks with polycaprolactone fibers are employed to spatially expand and mechanically strengthen fibrous matrices. Iron ions play a role as a catalyst for rapid formation of irreversible dopamine polymerization. The adhesive catecholamine reinforces the mechanical properties of 3D electrospun fibers and triggers the robust immobilization of biomolecules, ultimately demonstrating their potential as drug or cell delivery vehicles.

    3. The Effect of Sterilization Methods on the Structural and Chemical Properties of Fibrin Microthread Scaffolds

      Jonathan M. Grasman, Megan P. O'Brien, Kevin Ackerman, Keith A. Gagnon, Gregory M. Wong and George D. Pins

      Article first published online: 4 FEB 2016 | DOI: 10.1002/mabi.201500410

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      The structural and chemical properties of fibrin-based scaffolds are affected by crosslinking and sterilization protocols. Ethanol and ethylene oxide gas sterilization procedures are performed on uncrosslinked, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), or ultraviolet light crosslinked fibrin microthreads to evaluate the mechanical, structural, and chemical properties of these materials. The data presented here inform the strategic design of fibrin-based scaffolds.

    4. Crosslinked Redox-Responsive Micelles Based on Lipoic Acid-Derived Amphiphiles for Enhanced siRNA Delivery

      Ariane Tschiche, Bala N. S. Thota, Falko Neumann, Andreas Schäfer, Nan Ma and Rainer Haag

      Article first published online: 4 FEB 2016 | DOI: 10.1002/mabi.201500363

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      Disulfide-based reversibly stabilized micelles based on crosslinkable lipoic acid amphiphiles are developed in the present study, which show degradation upon reductive conditions, and thus lead to efficient cargo release. In particular, one candidate out of this series is able to efficiently release siRNA due to its redox-responsive biodegradable profile, thereby leading to potent gene silencing.

    5. pH-Triggered Charge-Reversal Polyurethane Micelles for Controlled Release of Doxorubicin

      Wanying He, Xu Zheng, Qi Zhao, Lijie Duan, Qiang Lv, Guang Hui Gao and Shuangjiang Yu

      Article first published online: 3 FEB 2016 | DOI: 10.1002/mabi.201500358

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      A pH-triggered charge-reversal nanocarrier is fabricated for efficient anticancer-drug delivery. The charge-reversal behavior is tunable by changing the molar ratio of piperazine/carboxyl. With reducing the pH, the surface charge of nanocarriers can be changed from negative to positive which enhance the cellular uptake and intracellular drug release.

    6. Biodegradable Self-Assembled Nanoparticles of Galactose-Containing Amphiphilic Triblock Copolymers for Targeted Delivery of Paclitaxel to HepG2 Cells

      Tieshi Wang, Xinde Tang, Jingtian Han, Yuanyuan Ding, Wenjuan Guo and Meishan Pei

      Article first published online: 2 FEB 2016 | DOI: 10.1002/mabi.201500413

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      Biodegradable self-assembled nanoparticles of galactose-containing amphiphilic triblock copolymers are used as carriers of paclitaxel to achieve targeted delivery to HepG2 cells. The polymeric NPs are biocompatible and non-toxic, and can be selectively recognized.

  2. Communications

    1. A Supramolecular Approach toward Bioinspired PAMAM-Dendronized Fusion Toxins

      Seah Ling Kuan, Christina Förtsch, David Yuen Wah Ng, Stephan Fischer, Yu Tokura, Weina Liu, Yuzhou Wu, Kaloian Koynov, Holger Barth and Tanja Weil

      Article first published online: 2 FEB 2016 | DOI: 10.1002/mabi.201500417

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      Supramolecular fusion toxins, which involve the C3 protein from Clostridium botulinum noncovalently fused to polyamidoamine-dendronized transporter proteins are prepared using a bottom-up molecular self-assembly, which allows the uptake behavior of the toxin to be reprogrammed and holds great promise as an innovative chemical tool for re-engineering protein therapeutics.

  3. Full Papers

    1. Acid Sensitive Polymeric Micelles Combining Folate and Bioreducible Conjugate for Specific Intracellular siRNA Delivery

      Yanfang Yang, Xuejun Xia, Wujun Dong, Hongliang Wang, Lin Li, Panpan Ma, Wei Sheng, Xueqing Xu and Yuling Liu

      Article first published online: 29 JAN 2016 | DOI: 10.1002/mabi.201500389

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      A new siRNA transporting system combining triple effects of folate targeting, acid sensitive polymer micelles, and bio-reducible disulfide bond linked siRNA-CPPs conjugate is developed to suppress c-myc gene expression of breast cancer both in vitro and in vivo. Good body safety and higher gene silencing efficiency of the constructed nanocarrier demonstrate it may be a potential siRNA transporting vector for oncotherapy.

    2. You have full text access to this OnlineOpen article
      Quantitative Comparison of the Antimicrobial Efficiency of Leaching versus Nonleaching Polymer Materials

      Joerg Bruenke, Ingolf Roschke, Seema Agarwal, Thomas Riemann and Andreas Greiner

      Article first published online: 25 JAN 2016 | DOI: 10.1002/mabi.201500266

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      Antimicrobial materials are of outmost importance for a variety of biomedical applications. The use of nonleaching antimicrobial materials offers a variety of obvious advantages for biomedical applications. In this contribution, a quantitative comparison of the antimicrobial activity of nonleaching and leaching antimicrobial polymer materials is undertaken by use of the well-established Certika test on central venous catheters.

    3. Mixed Micelles for Targeted and Efficient Doxorubicin Delivery to Multidrug-Resistant Breast Cancer Cells

      Heesun Jung and Hyejung Mok

      Article first published online: 25 JAN 2016 | DOI: 10.1002/mabi.201500381

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      In this report, mixed micelles composed of biocompatible polypropylene glycol (PPG)-grafted HA copolymers (PPG-g-HA) and pluronic L61 are proposed, named them as PPG-g-HA/L61 micelles, for targeted delivery to multidrug resistance (MDR) breast cancer cells (MCF-7 MDR cells). This study reveals that the PPG-g-HA/L61 micelles can be a promising nanocarrier to overcome MDR and release doxorubicin in a hyaluronidase-sensitive manner without any toxicity to normal cells.

    4. Polydopamine-Decorated Sticky, Water-Friendly, Biodegradable Polycaprolactone Cell Carriers

      Minhee Kim, Jung-Suk Kim, Haeshin Lee and Jae-Hyung Jang

      Article first published online: 22 JAN 2016 | DOI: 10.1002/mabi.201500432

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      Polydopamine interfacial glue can create a highly versatile platform cell carrier that can maximize the therapeutic benefits of cell replacement approaches. The polydopamine coating on polycaprolactonemicrospheres devised sticky, water-friendly, and biodegradable cell carriers, where cells are robustly immobilized on their external surfaces. The sticky cell carrier demonstrates great potential to provide crucial clues to implement the significant advancement of cell therapy approaches.

    5. Poly(p-phenylene) with Poly(ethylene glycol) Chains and Amino Groups as a Functional Platform for Controlled Drug Release and Radiotherapy

      Bahar Guler, Huseyin Akbulut, Firat Baris Barlas, Caner Geyik, Dilek Odaci Demirkol, Ahmet Murat Senisik, Halil Armagan Arican, Hakan Coskunol, Suna Timur and Yusuf Yagci

      Article first published online: 21 JAN 2016 | DOI: 10.1002/mabi.201500384

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      Poly(p-phenylene) with poly(ethylene glycol) chains and amino groups is used as a cargo molecule for the as controlled release of anti-cancer drug and radiotherapy applications.

    6. Catechin-Modified Polylactide Stereocomplex at Chain End Improved Antibiobacterial Property

      Hiroharu Ajiro, Shogo Ito, Kai Kan and Mitsuru Akashi

      Article first published online: 20 JAN 2016 | DOI: 10.1002/mabi.201500398

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      Chain end moiety of polylactides are modified with chemical species bearing antibacterial properties, using initiators. The double bonds are introduced in order to incorporate silver ion by physical interaction, resulting in a poor association. On the other hand, chemically bonded catechin shows antibacterial properties, as well as the improved thermal resistance by stereocomplex formation.

    7. Electrospun Poly(ε-caprolactone)/Polyhedral Oligomeric Silsesquioxane-Based Copolymer Blends: Evolution of Fiber Internal Structures

      Adam J. P. Bauer, Yitian Wu and Bingbing Li

      Article first published online: 19 JAN 2016 | DOI: 10.1002/mabi.201500399

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      This study reports a comprehensive, morphological, physicochemical, and structural evaluation of electrospun poly(ε-caprolactone) (PCL)/poly[(propylmethacrylheptaisobutyl-polyhedral oligomeric silsesquioxane)-co-(methyl methacrylate)] (POSS-MMA) fibers. A ternary phase diagram is constructed to depict the electrospinnability of solution mixtures. A transition from PCL-rich fibers, to bicontinuous core/shell fibers, to POSS-MMA-rich fibers with a discontinuous PCL inner phase is clearly evidenced by both physicochemical characterization and structural analyses.

    8. Gelatin Nanoparticles with Enhanced Affinity for Calcium Phosphate

      Kambiz Farbod, Mani Diba, Tatiana Zinkevich, Stephan Schmidt, Matthew J. Harrington, Arno P. M. Kentgens and Sander C. G. Leeuwenburgh

      Article first published online: 15 JAN 2016 | DOI: 10.1002/mabi.201500414

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      Targeted drug delivery entails a selective and effective localization of pharmacologically active compounds at predefined targeted site(s), thus minimizing undesired side effects. By means of conjugating bone-targeting alendronate to biocompatible gelatin nanoparticles, a simple method is developed for the preparation of gelatin nanoparticles that exhibit great potential for use in bone-specific drug delivery and regenerative medicine.

    9. Amphiphilic Copolymers Shuttle Drugs Across the Blood–Brain Barrier

      Mirjam Clemens-Hemmelmann, Christiane Kuffner, Verena Metz, Linda Kircher, Ulrich Schmitt, Christoph Hiemke, Rolf Postina and Rudolf Zentel

      Article first published online: 13 JAN 2016 | DOI: 10.1002/mabi.201500388

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      The non-water-soluble and brain-impermeable drug domperidone (DOM) can be enriched in the brain by use of an amphiphilic copolymer based on poly(N-(2-hydroxypropyl)-methacrylamide) as a carrier.

    10. Effect of PCL/PEG-Based Membranes on Actinorhodin Production in Streptomyces coelicolor Cultivations

      Roberto Scaffaro, Francesco Lopresti, Alberto Sutera, Luigi Botta, Rosa Maria Fontana, Anna Maria Puglia and Giuseppe Gallo

      Article first published online: 13 JAN 2016 | DOI: 10.1002/mabi.201500391

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      Polycaprolactone-based membranes are developed for cultivations of Streptomyces coelicolor, a model strain producing various secondary metabolites including the blue colored actinorhodin (ACT). The results indicate that the ACT production is strongly dependent on the pores size distribution of the device. In particular, membranes with pores of 100 μm are able to offer a potential improvement in volumetric productivity of ACT if compared to conventional submerged liquid culture.

    11. Silk Fibroin Degradation Related to Rheological and Mechanical Properties

      Benjamin P. Partlow, A. Pasha Tabatabai, Gary G. Leisk, Peggy Cebe, Daniel L. Blair and David L. Kaplan

      Article first published online: 12 JAN 2016 | DOI: 10.1002/mabi.201500370

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      Silk fibroin is a promising natural bio­material that is useful for numerous applications. However, a full understanding of how processing affects the final material characteristics is required to effectively engineer devices and scaffolds. The degree of degradation, due to processing, is shown to have a strong influence on both solution and solid state properties.

    12. Three-Layered PCL Grafts Promoted Vascular Regeneration in a Rabbit Carotid Artery Model

      Kai Wang, Wenting Zheng, Yiwa Pan, Shaoyang Ma, Yong Guan, Ruming Liu, Meifeng Zhu, Xin Zhou, Jun Zhang, Qiang Zhao, Yan Zhu, Lianyong Wang and Deling Kong

      Article first published online: 12 JAN 2016 | DOI: 10.1002/mabi.201500355

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      A middle loose layer is created in preparation of vascular grafts by electrospinning poly(ε-caprolactone) and electrospraying poly(ethylene oxide). The presence of loose layer overcomes the limitation of small pores in the regular electrospun scaffolds, significantly enhanced macrophage infiltration, cellularization, and blood vessel regeneration in rabbit carotid artery implantation.

    13. Scanning Ion Conductance Microscopic Study for Cellular Uptake of Cationic Conjugated Polymer Nanoparticles

      Yuping Shan, Namuna Panday, Yong Myoung, Megan Twomey, Xuewen Wang, Wenzhi Li, Emrah Celik, Vincent Moy, Hongda Wang, Joong Ho Moon and Jin He

      Article first published online: 12 JAN 2016 | DOI: 10.1002/mabi.201500320

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      Scanning ion conductance microscopy (SICM) reveals that high surface area ratio changes are observed in human cervical cancer cells incubated with positively charged conjugated polymer nanoparticles (CPNs), implying active cell surface responses toward adsorbed CPNs.

    14. Maleimide-Functionalized Poly(2-Oxazoline)s and Their Conjugation to Elastin-Like Polypeptides

      Jonas F. Nawroth, Jonathan R. McDaniel, Ashutosh Chilkoti, Rainer Jordan and Robert Luxenhofer

      Article first published online: 12 JAN 2016 | DOI: 10.1002/mabi.201500376

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      A novel method is described to incorporate maleimide functionality into poly(2-oxazoline)s via nucleophilic termination with the Diels–Alder adduct of furan and maleimide. With this functionality, a new peptide-polymer hybrid of hydrophilic cysteine-bearing Elastin-like Polypeptides and hydrophobic poly(2-oxazoline)s is created. These conjugates spontaneously form aggregates in water and are used in first experiments for loading of anticancer drug paclitaxel.

    15. Polymicrobial Biofilm Inhibition Effects of Acetate-Buffered Chitosan Sponge Delivery Device

      Jessica Amber Jennings, Karen E. Beenken, Ashley C. Parker, James Keaton Smith, Harry S. Courtney, Mark S. Smeltzer and Warren O. Haggard

      Article first published online: 12 JAN 2016 | DOI: 10.1002/mabi.201500347

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      Degradable chitosan sponges modified with an acetate buffer release two different antibiotics, vancomycin and amikacin, to prevent polymicrobial biofilm infection by Staphylococcus aureus and Pseudomonas aeruginosa.

    16. Effects of Grafting Density and Film Thickness on the Adhesion of Staphylococcus epidermidis to Poly(2-hydroxy ethyl methacrylate) and Poly(poly(ethylene glycol)methacrylate) Brushes

      Sorin-Alexandru Ibanescu, Justyna Nowakowska, Nina Khanna, Regine Landmann and Harm-Anton Klok

      Article first published online: 12 JAN 2016 | DOI: 10.1002/mabi.201500335

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      This study investigates the influence of grafting density and film thickness on the adhesion of Staphylococcus epidermidis to poly(poly(ethylene glycol)methacrylate) and poly(2-hydroxyethyl methacrylate) brushes prepared via surface-initiated polymerization. Except for very low grafting densities (ρ = 1%), these polymer brushes are equally effective as poly(ethylene glycol)-modified surfaces in preventing S. epidermis adhesion, independent on film thickness and grafting density.

    17. Mimicking of Chondrocyte Microenvironment Using In Situ Forming Dendritic Polyglycerol Sulfate-Based Synthetic Polyanionic Hydrogels

      Pradip Dey, Tobias Schneider, Leonardo Chiappisi, Michael Gradzielski, Gundula Schulze-Tanzil and Rainer Haag

      Article first published online: 11 JAN 2016 | DOI: 10.1002/mabi.201500377

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      A stable polyanionic injectable hydrogel with varying amount of the negatively charged polymer have been developed using a bioorthogonal strain promoted azide-alkyne cycloaddition reaction. Introduction of negatively charged polymers leads to better chondrocyte viability over 21 d compared to the neutral hydrogels and also shows less collagen type I gene deposition.

    18. You have full text access to this OnlineOpen article
      A Selective Mucin/Methylcellulose Hybrid Gel with Tailored Mechanical Properties

      Constantin Nowald, Anja Penk, Hsin-Yi Chiu, Thomas Bein, Daniel Huster and Oliver Lieleg

      Article first published online: 8 JAN 2016 | DOI: 10.1002/mabi.201500353

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      Mucin/MC hybrid gels combine thermal autogelation behavior with selective permeability properties. The hybrid system quickly forms a viscoelastic gel upon contact with warm body surfaces. By integrating thermoresponsive liposomes into the gel, an increase in temperature triggering the gelation process can—at the same time—induce delayed and prolonged drug release.

  4. Reviews

    1. Recent Strategies in Tissue Engineering for Guided Peripheral Nerve Regeneration

      Kayla Belanger, Tony M. Dinis, Sami Taourirt, Guillaume Vidal, David L. Kaplan and Christopher Egles

      Article first published online: 8 JAN 2016 | DOI: 10.1002/mabi.201500367

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      Treating severe peripheral nerve injuries remains a clinical challenge to this day. The “gold standard” for surgical intervention of a sectioned nerve has long been the autologous nerve graft, but with the continuing advancement in the field of tissue engineering, safer and more economical alternatives utilizing synthetic and natural polymers are becoming available.

  5. Full Papers

    1. Biocompatible Size-Defined Dendrimer–Albumin Binding Protein Hybrid Materials as a Versatile Platform for Biomedical Applications

      Jan Maly, Ondrej Stanek, Jan Frolik, Marek Maly, Franka Ennen, Dietmar Appelhans, Alena Semeradtova, Dominika Wrobel, Marcel Stofik, Tereza Knapova, Milan Kuchar, Lucie Cervenkova Stastna, Jan Cermak, Peter Sebo and Petr Maly

      Article first published online: 8 JAN 2016 | DOI: 10.1002/mabi.201500332

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      Biotinylated dendrimer is used for a controlled fabrication of biocompatible biohybrid structures to undergo conjugation with a highly HSA-affine tetrameric streptavidin-binding domain protein scaffold. The biohybrid structures are suitable as a potential ligand-tailored drug delivery system, where the dendrimer can render a specifically high affinity to various biological ligands and biomolecules based on the binding property of the mutated binding domain proteins.

    2. Tuning the Mechanical Properties of Poly(Ethylene Glycol) Microgel-Based Scaffolds to Increase 3D Schwann Cell Proliferation

      Wenda Zhou, Jessica M. Stukel, Hannah L. Cebull and Rebecca Kuntz Willits

      Article first published online: 4 JAN 2016 | DOI: 10.1002/mabi.201500336

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      A novel, microgel-based scaffold is developed and characterized to manipulate the range of stiffnesses appropriate for neural tissue engineering. The results demonstrate here an optimal stiffness range for increased Schwann cell proliferation that is 5× lower than previously reported for 2D studies.

    3. Designing Visible Light-Cured Thiol-Acrylate Hydrogels for Studying the HIPPO Pathway Activation in Hepatocellular Carcinoma Cells

      Tsai-Yu Lin, John C. Bragg and Chien-Chi Lin

      Article first published online: 28 DEC 2015 | DOI: 10.1002/mabi.201500361

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      Dually degradable PEG-peptide hydrogels are synthesized using visible light initiated thiol-acrylate photopolymerization. Methods of modulating hydrogel crosslinking and degradation are proposed, including adjusting concentration of comonomer or altering the design of peptide linker. This diverse hydrogel system is useful in studying the influence of matrix stiffness and integrin ligands on cell fate processes in 3D.

    4. Selective Uptake of a Fructose Glycopolymer Prepared by RAFT Polymerization into Human Breast Cancer Cells

      Christian von der Ehe, Alexandra Rinkenauer, Christine Weber, David Szamosvari, Michael Gottschaldt and Ulrich S. Schubert

      Article first published online: 21 DEC 2015 | DOI: 10.1002/mabi.201500346

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      A new fructose glycomonomer is copoly­merized with N-isopropyl acrylamide by reversible addition fragmentation chain transfer (RAFT) polymerization. The fructose glycopolymer as well as the analog mannose, glucose, and galactose glycopoly­mers are deprotected and labeled with a Rhodamine B derivative. The glycopolymers are noncytotoxic and show uptake into MDA-MB-231 breast cancer cells, revealing the highest uptake efficiency for the fructose polymer.

    5. Folic-Acid-Modified Conducting Polymer: Electrochemical Detection of the Cell Attachment

      Hacer Azak, Firat Baris Barlas, Huseyin Bekir Yildiz, Kadri Gulec, Bilal Demir, Dilek Odaci Demirkol and Suna Timur

      Article first published online: 15 DEC 2015 | DOI: 10.1002/mabi.201500274

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      Electropolymerization of 4-[4H-dithieno(3,2-b:2′,3′-d)pyrrol-4-yl]aniline (DTP-aryl-NH2) is carried out and folic acid was modified via functional amino groups on resulted conducting polymer. This biorecognition layer is conducted to the cell attachment and detection applications by using HeLa (folate receptor positive) and A549 (folate receptor negative) cell lines.

    6. Enhanced Cellular Activity in Gelatin-Poly(Ethylene Glycol) Hydrogels without Compromising Gel Stiffness

      Thai Thanh Hoang Thi, Jung Seok Lee, Yunki Lee, Kyung Min Park and Ki Dong Park

      Article first published online: 14 DEC 2015 | DOI: 10.1002/mabi.201500327

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      Cells are incorporated within two types of stiff hydrogels. The hydrogel consisting of only semi-synthetic polymer (GPT) suppresses cellular activities, while composite hydrogel of GPT and gelatin-hydroxyphenyl propionic acid conjugates (natural polymer) induces adhesion and proliferation of embedded cell. The live/dead staining images show that the human dermal fibroblasts cultured in composite hydrogel matrix after 10 days are spread out while those in GPT hydrogel are in a quiescent state with a round morphology. This phenomenon demonstrates that deficiency in cellular activity driven by gel stiffness could be overcome by finely tuning the material properties.

    7. Poly(Amido Amine)s Containing Agmatine and Butanol Side Chains as Efficient Gene Carriers

      Young-Wook Won, Marc Ankoné, Johan F. J. Engbersen, Jan Feijen and Sung Wan Kim

      Article first published online: 11 DEC 2015 | DOI: 10.1002/mabi.201500369

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      Poly cystamine bisacrylamide with 4-aminobutanol and 4-aminobutylguanidine (agmatine) (CBA-ABOL/AGM) containing 80% AGM and 20% ABOL has shown the best transfection efficiency with the lowest cytotoxicity, indicating that this polymer is very promising as a potent and nontoxic gene carrier.

    8. Polyelectrolytes Multilayers to Modulate Cell Adhesion: A Study of the Influence of Film Composition and Polyelectrolyte Interdigitation on the Adhesion of the A549 Cell Line

      Nicolás E. Muzzio, Miguel A. Pasquale, Danijela Gregurec, Eleftheria Diamanti, Marija Kosutic, Omar Azzaroni and Sergio E. Moya

      Article first published online: 11 DEC 2015 | DOI: 10.1002/mabi.201500275

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      Biocompatible surfaces with improved cell adhesion are obtained by diblock polyelectrolyte multilayers (PEMs). On the basis of cell adhesion studied on a large number of PEMs made of natural and synthetic polyelectrolytes, a diblock PEM is fabricated by combining a block of soft natural polyelectrolytes on the top of a stiffer block containing poly(sodium 4-styrenesulfonate) as polyanion.

    9. In Vivo Imaging of Glycol Chitosan-Based Nanogel Biodistribution

      Paula Pereira, Alexandra Correia and Francisco M. Gama

      Article first published online: 10 DEC 2015 | DOI: 10.1002/mabi.201500267

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      It is primordial to study the biosdistribution profile of nanogels following systemic administration for the preclinical development of nanosystems; the in vivo performance will determine its feasibility as a nanomedicine. Near-infrared fluorescence imaging allows concluding that the nanogels have a satisfactory blood circulation half-life, suitable for drug delivery, and simultaneously an efficient and quick body clearance.

    10. Reduction-Sensitive Polymeric Micelles Based on Docetaxel-Polymer Conjugates Via Disulfide Linker for Efficient Cancer Therapy

      Yuanyuan Guo, Pei Zhang, Qingyun Zhao, Kaiming Wang and Yuxia Luan

      Article first published online: 9 DEC 2015 | DOI: 10.1002/mabi.201500317

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      The synthesized mPEG-PLGA-SS-DTX conjugates are used to load free DTX to conduct dual-mode drug loaded polymeric micelles. The reduction-sensitive PP-SS-DTX/DTX micelles are passively targeted to tumor tissues and release anticancer drugs at the reductive glutathione condition in tumor cells which is important in drug concentrating to tumors. The as-prepared micelles exhibit effective activity on inhibiting cancer cells for promising cancer therapy application.

    11. Polyglycerol-Based Copper Chelators for the Transport and Release of Copper Ions in Biological Environments

      Ralf Albrecht, Susanne Fehse, Kritee Pant, Sabrina Nowag, Holger Stephan, Rainer Haag and Carl Christoph Tzschucke

      Article first published online: 8 DEC 2015 | DOI: 10.1002/mabi.201500284

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      Multifunctionalized polymer nanoparticles with hyperbranched polyglycerol as core and an outer shell of ligands can be used for stabilization and transport of copper in biological environments. We investigate the copper loading capacity, the stability of the copper loaded carriers at different pHs, in the presence of ethylenediaminetetraacetic acid and in human serum, as well as the transport of copper in biological systems. A cyclam-based carrier system shows promising results.

    12. Mitochondrially Targeted Nanoparticles Based on α-TOS for the Selective Cancer Treatment

      Raquel Palao-Suay, Laura Rodrigáñez, María Rosa Aguilar, Carolina Sánchez-Rodríguez, Francisco Parra, Mar Fernández, Juan Parra, Juan Riestra-Ayora, Ricardo Sanz-Fernández and Julio San Román

      Article first published online: 3 DEC 2015 | DOI: 10.1002/mabi.201500265

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      Pseudo-block copolymers based on copolymers of N-vinyl pyrrolidone and a methacrylic derivative of α-tocopheryl succinate (α-TOS) are used to encapsulate additional α-TOS and the resulted nanoparticles (NPs) present a potent activity and selectivity against cancer cells. Both unloaded and loaded NPs reduce cell viability of cancer cells by the induction of apoptosis (intrinsic pathway).

    13. Zwitterionic Cellulose Carbamate with Regioselective Substitution Pattern: A Coating Material Possessing Antimicrobial Activity

      Thomas Elschner, Claudia Lüdecke, Diana Kalden, Martin Roth, Bettina Löffler, Klaus D. Jandt and Thomas Heinze

      Article first published online: 3 DEC 2015 | DOI: 10.1002/mabi.201500349

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      A polyzwitterion with regioselective distribution of positive and negative charges is synthesized applying the platform chemistry of cellulose phenyl carbonate. Coatings of the biopolymer derivative on glass, titanium, and tissue culture poly(styrene) possess an antimicrobial activity against E. coli.

    14. Effect of Immobilized Thiolated Glycosaminoglycans on Fibronectin Adsorption and Behavior of Fibroblasts

      Alexander Köwitsch, Marcus S. Niepel, Georgios P. A. Michanetzis, Yannis F. Missirlis and Thomas Groth

      Article first published online: 30 NOV 2015 | DOI: 10.1002/mabi.201500276

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      Glycosaminoglycans (GAGs) with different degrees of sulfation are thiolated for covalent immobilization on functionalized model substrata to enable biospecific interactions. The modified surfaces show prominent differences in fibronectin adsorption and evoke discrete adhesion mechanisms of human fibroblasts depending on the degree of sulfation and thiolation of the applied GAGs.

  6. Communications

    1. Surface Charge Convertible and Biodegradable Synthetic Zwitterionic Nanoparticles for Enhancing Cellular Drug Uptake

      Luyan Wu, Caihua Ni, Liping Zhang, Gang Shi, Xue Bai, Yamin Zhou and Fei He

      Article first published online: 30 NOV 2015 | DOI: 10.1002/mabi.201500299

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      A biodegradable terpolymer is synthesized, leading to the formation of zwitter­ionic nanoparticles through self-assembly. The surface charge is convertible from negative to positive at pH 7.4 and 6.5, respectively. As drug vehicles the NPs display excellent nonfouling feature in bloodstream and effective anticancer drug uptake in tumor cells.

  7. Full Papers

    1. A Supramolecular Gel Approach to Minimize the Neural Cell Damage during Cryopreservation Process

      Jie Zeng, Yixia Yin, Li Zhang, Wanghui Hu, Chaocan Zhang and Wanyu Chen

      Article first published online: 27 NOV 2015 | DOI: 10.1002/mabi.201500277

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      A thermoreversible supramolecular gel cryopreservation system increases the viabilities of cells during cryopreservation since it confines ice crystal growth in its porous network and it reduces the change rate of osmotic shock when the cryoprotective agent is loaded and unloaded. Moreover, this supramolecular gel can be removed easily so it does not hinder the adherence, growth, and proliferation of thawed cells.

    2. Development of Non-Cell Adhesive Vascular Grafts Using Supramolecular Building Blocks

      Geert C. van Almen, Hanna Talacua, Bastiaan D. Ippel, Björne B. Mollet, Mellany Ramaekers, Marc Simonet, Anthal I. P. M. Smits, Carlijn V. C. Bouten, Jolanda Kluin and Patricia Y. W. Dankers

      Article first published online: 27 NOV 2015 | DOI: 10.1002/mabi.201500278

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      This proof-of-concept study demonstrates that mixing of ureido-pyrimidinone (UPy) modified poly(ethylene glycol) (UPy-PEG) with a chain-extended UPy-functionalized polycaprolactone results in a mechanically stable material that can be electrospun into vascular tubular grafts. It is shown that only 10% of this UPy-PEG is sufficient to obtain reduced cell infiltration in vivo, after a relatively short implantation time.

    3. Two-Phase Electrospinning to Incorporate Polyelectrolyte Complexes and Growth Factors into Electrospun Chitosan Nanofibers

      Laura W. Place, Maria Sekyi, Julia Taussig and Matt J. Kipper

      Article first published online: 27 NOV 2015 | DOI: 10.1002/mabi.201500288

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      Three methods are described for electrospinning chitosan nanofibers containing heparin/growth factor nanoparticles in a separate phase. Electrospinning a water-in-organic emulsion does not achieve growth factor incorporation. Electrospinning nanofibers using a compound needle with a chitosan organic phase and a poly(vinyl alcohol) aqueous phase achieves the most successful growth factor incorporation and delivery, when the aqueous phase is in the core.

    4. Paclitaxel-Loaded β-Cyclodextrin-Modified Poly(Acrylic Acid) Nanoparticles through Multivalent Inclusion for Anticancer Therapy

      Shanmei Yuan, Jiao Chen, Jie Sheng, Yong Hu and Zhongying Jiang

      Article first published online: 27 NOV 2015 | DOI: 10.1002/mabi.201500302

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      Paclitaxel-loaded multivalent inclusion nanoparticles (NPs) are fabricated. These obtained NPs have a negative surface charge and show an enhanced paclitaxel (PTX) solubility. They can accumulate in the tumor tissue and have a superior antitumor activity compared to commercially available Taxol, which indicates that these NPs own potential application in the PTX delivery system.

    5. Synthesis of Novel Cellulose Carbamates Possessing Terminal Amino Groups and Their Bioactivity

      Kristin Ganske, Cornelia Wiegand, Uta-Christina Hipler and Thomas Heinze

      Article first published online: 27 NOV 2015 | DOI: 10.1002/mabi.201500324

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      Novel cellulose carbamates are synthesized by aminolysis of cellulose phenyl carbonates. The ω-aminoethyl-p-aminobenzylcellulose carbamate shows antimicrobial activity and a moderate biocompatibility not only in solution but also as films on cotton and polyester. Cotton coated with cellulose carbamate inhibits bacteria growth in case of Staphylococcus aureus and Klebsiella pneumoniae completely.

    6. Biofunctionalized Hydrogel Microscaffolds Promote 3D Hepatic Sheet Morphology

      Myung Hee Kim, Supriya K. Kumar, Hitomi Shirahama, Jeongeun Seo, Jae-Ho Lee, Vladimir P. Zhdanov and Nam-Joon Cho

      Article first published online: 27 NOV 2015 | DOI: 10.1002/mabi.201500338

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      Spheroid hepatocyte culture, as found in sphere-templated nonfouling microscaffolds, poses the risk of cell necrosis at the hepatosphere core. Conjugating extracellular matrix (ECM) to these porous scaffolds directs heptatocytes into an advantageous 3D sheet morphology, shown here both experimentally and via Monte Carlo simulation.

    7. Application of Linear and Branched Poly(Ethylene Glycol)-Poly(Lactide) Block Copolymers for the Preparation of Films and Solution Electrospun Meshes

      Martina Kessler, Juergen Groll and Joerg Tessmar

      Article first published online: 27 NOV 2015 | DOI: 10.1002/mabi.201500238

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      Star-shaped poly(ethylene glycol)-poly­(lactide) block copolymers are processed to solvent cast films and solution electrospun meshes. Their swelling, degradation, and mechanical properties are investigated regarding an application as barrier device for the prevention of postsurgical adhesions. With increased branching of the polymer a longer retention of PEG and a longer endurance of the mechanical properties are achieved.

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