Macromolecular Bioscience

Cover image for Vol. 15 Issue 2

Early View (Online Version of Record published before inclusion in an issue)

VIEW

  1. 1 - 44
  1. Full Papers

    1. Synthesis and Properties of Star HPMA Copolymer Nanocarriers Synthesised by RAFT Polymerisation Designed for Selective Anticancer Drug Delivery and Imaging

      Petr Chytil, Eva Koziolová, Olga Janoušková, Libor Kostka, Karel Ulbrich and Tomáš Etrych

      Article first published online: 2 MAR 2015 | DOI: 10.1002/mabi.201400510

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      Well-defined water-soluble high-molecular-weight star polymer drug nanocarriers, based on HPMA copolymers, enabling the pH-sensitive drug release in the tumour tissue were synthesised and their properties were studied. The nanocarriers showed a low dispersity and contained different functionalities, which enabled the attachment of drugs along the polymer chains and also the one-point attachment for the targeting ligands and/or a labelling moiety.

    2. Inclusion Complexes Between Polytetrahydrofuran-b-Amylose Block Copolymers and Polytetrahydrofuran Chains

      Rachmawati Rachmawati, Albert J. J. Woortman, Kamlesh Kumar and Katja Loos

      Article first published online: 23 FEB 2015 | DOI: 10.1002/mabi.201400515

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      Polytetrahydrofuran-block-amylose is able to act as a host molecule which can include guest polytetrahydrofuran chains to result in complexes. The amylose helices in the resulting complexesare in the form of a V amylose with six glucose residues per helical turn. This complexation between the host block copolymer and the guest homopolymer offers a facile route to building supramolecular structures.

    3. Optimization of Aqueous SI-ATRP Grafting of Poly(Oligo(Ethylene Glycol) Methacrylate) Brushes from Benzyl Chloride Macroinitiator Surfaces

      Andrew E. Rodda, Francesca Ercole, David R. Nisbet, John S. Forsythe and Laurence Meagher

      Article first published online: 17 FEB 2015 | DOI: 10.1002/mabi.201400512

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      Surface Initiated ATRP of poly(OEGMA) was performed from macroinitiators bearing the stable benzyl chloride moiety. Despite the structural mismatch between the initiator and monomer and expected poor kinetics, dense polymer brush coatings were grafted from the surface under a set of optimised conditions in aqueous solution. These coatings were highly effective at reducing protein fouling and cell adhesion.

    4. Organic Radical Contrast Agents Based on Polyacetylenes Containing 2,2,6,6-Tetramethylpiperidine 1-Oxyl (TEMPO): Targeted Magnetic Resonance (MR)/Optical Bimodal Imaging of Folate Receptor Expressing HeLa Tumors in Vitro and in Vivo

      Lixia Huang, Chenggong Yan, Danting Cui, Yichen Yan, Xiang Liu, Xinwei Lu, Xiangliang Tan, Xiaodan Lu, Jun Xu, Yikai Xu and Ruiyuan Liu

      Article first published online: 13 FEB 2015 | DOI: 10.1002/mabi.201400403

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      Folic acid modified paramagnetic fluorescent bimodal organic radical contrast agents (ORCAs) are prepared using polyacetylenes containing 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO). The ORCAs exhibit satisfactory magnetic resonance imaging (MRI) contrast efficacy with low cytotoxicity. In vitro and in vivo MRI/optical imaging demonstrate the active targetability of the targeted ORCAs to folate receptor-positive HeLa tumors.

  2. Feature Articles

    1. Cellulose Carbonates: A Platform for Promising Biopolymer Derivatives With Multifunctional Capabilities

      Thomas Elschner and Thomas Heinze

      Article first published online: 13 FEB 2015 | DOI: 10.1002/mabi.201400521

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      Cellulose carbonates are versatile platform compounds in order to obtain highly engineered, functional biopolymer derivatives. Low, intermediate, and high degrees of substitution as well as regioselective substitution patterns are accessible.

  3. Full Papers

    1. Galactosylated Poly(Ethyleneglycol)-Lithocholic Acid Selectively Kills Hepatoma Cells, While Sparing Normal Liver Cells

      Nomundelger Gankhuyag, Bijay Singh, Sushila Maharjan, Yun-Jaie Choi, Chong-Su Cho and Myung-Haing Cho

      Article first published online: 6 FEB 2015 | DOI: 10.1002/mabi.201400475

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      A potent anti-cancer drug, lithocholic acid (LCA), is converted to poly(ethyleneglycol) (PEG) conjugates, and further decorated with lactobionic acid (LBA) to produce LCA-PEG-LBA (LPL) to target the hepatocytes. LPL can self-assemble to form nanoparticles, and it has high potency than LCA to kill HepG2 cancer cells, sparing normal LO2 cells. The cell death is occurred through apoptosis induced by LPL nanoparticles. Besides, LPL has high specificity to mouse liver cells in vivo.

  4. Reviews

    1. The Role of Matrix Compliance on Cell Responses to Drugs and Toxins: Towards Predictive Drug Screening Platforms

      Silviya Petrova Zustiak

      Article first published online: 5 FEB 2015 | DOI: 10.1002/mabi.201400507

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      The compliance of the cellular environment influences cell responses to drugs. When cells are seeded on 2D substrates or in 3D matrices of physiologically-relevant stiffness or on tissue culture polystyrene (TCP) and subjected to a drug screen, they exhibit different drug sensitivity, where the stiffness-matched material resembles in vivo results more closely than those obtained on the TCP.

    2. Defined Polymeric Materials for Gene Delivery

      Dongsheng He and Ernst Wagner

      Article first published online: 5 FEB 2015 | DOI: 10.1002/mabi.201400524

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      Polymers with precise chemical structure will be useful gene delivery carriers. Due to the different extracellular and intracellular delivery barriers, multiple transport functions have to be incorporated in a site-specific manner into precise molecular backbones. Strategies for the design of such defined carrier materials, including dendrimers, peptides, and sequence-defined oligoaminoamides are reviewed.

  5. Full Papers

    1. Incorporation of Methionine Analogues Into Bombyx mori Silk Fibroin for Click Modifications

      Hidetoshi Teramoto and Katsura Kojima

      Article first published online: 2 FEB 2015 | DOI: 10.1002/mabi.201400482

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      Clicking the silk: Bombyx mori silk fibroin incorporating unnatural analogues of methionine (Met) is produced. No genetic manipulation of B. mori larvae is necessary for production. Selective modifications of incorporated Met analogues are possible with click chemistry. This study provides a novel approach to modifying the characteristics of silk-based biomaterials.

    2. Mucus Barriers to Microparticles and Microbes are Altered in Hirschsprung's Disease

      Hasan M. Yildiz, Taylor L. Carlson, Allan M. Goldstein and Rebecca L. Carrier

      Article first published online: 2 FEB 2015 | DOI: 10.1002/mabi.201400473

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      Microparticle and microbial transport through colonic mucus were reduced in an animal model of Hirschsprung's disease (HD) (7-fold and 3.6-fold, respectively). Mucus barrier differences extended beyond the aganglionic colon segment, consistent with development of enterocolitis after surgical removal of aganglionic colon. Therapies geared toward altering the mucus barrier could be explored for preventing HD associated enterocolitis.

    3. Three-Layered Biodegradable Micelles Prepared by Two-Step Self-Assembly of PLA-PEI-PLA and PLA-PEG-PLA Triblock Copolymers as Efficient Gene Delivery System

      Daniel G. Abebe, Rima Kandil, Teresa Kraus, Maha Elsayed, Olivia M. Merkel and Tomoko Fujiwara

      Article first published online: 2 FEB 2015 | DOI: 10.1002/mabi.201400488

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      “Three-layered micelles” (3LM) comprised of two of low MW triblock copolymers PLLA-PEI-PLLA and PLLA-PEG-PLLA are designed to combine electrostatic interaction and solvent-induced condensation of DNA. The 3LM shows efficient encapsulation of DNA, great stability at neutral pH and efficient DNA release at acidic pH (4.5). A new class of non-viral delivery systems for nucleic acids with superb stability and stealth properties is identified.

    4. Improved Gene Transfection Efficacy and Cytocompatibility of Multifunctional Polyamidoamine-Cross-Linked Hyaluronan Particles

      Akshay Srivastava, Claire Cunningham, Abhay Pandit and J. Gerard Wall

      Article first published online: 29 JAN 2015 | DOI: 10.1002/mabi.201400401

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      Multi-functional, cationic HA-based particles are developed by cross-linking HA chains with polyamidoamine (PAMAM). The well segregated, cationized particles complex plasmid DNA efficiently and can be functionalized with recombinant antibody fragments for cell targeting. They exhibit improved transfection efficiency and negligible cytotoxicity toward bovine intervertebral disk cells compared with Superfect® or non-cationized particles

    5. Facile Synthesis and Structural Characterization of Amylose–Fatty Acid Inclusion Complexes

      Zheng Cao, Albert J. J. Woortman, Petra Rudolf and Katja Loos

      Article first published online: 29 JAN 2015 | DOI: 10.1002/mabi.201400464

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      Amylose–fatty acid complexes can be prepared by mixing various fatty acids with amylose in hot aqueous solutions. Each fatty acid is selective for a specific fraction of amylose leading to a fractionation effect.

    6. Injectable Hybrid Hydrogels of Hyaluronic Acid Crosslinked by Well-Defined Synthetic Polycations: Preparation and Characterization In Vitro and In Vivo

      Daisy Cross, Xiaoze Jiang, Weihang Ji, Wenqing Han and Chun Wang

      Article first published online: 28 JAN 2015 | DOI: 10.1002/mabi.201400491

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      Hyaluronic acid and well-defined synthetic cationic copolymers form hybrid hydrogels through electrostatic interactions. These materials may have potential medical applications such as injectable carriers for cells.

    7. New Ionic bis-MPA and PAMAM Dendrimers: A Study of Their Biocompatibility and DNA-Complexation

      Julie Movellan, Rebeca González-Pastor, Pilar Martín-Duque, Teresa Sierra, Jesús M. de la Fuente and José Luis Serrano

      Article first published online: 28 JAN 2015 | DOI: 10.1002/mabi.201400422

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      Ionic dendrimers are described that consist on quaternary salts of amino-terminated PAMAM and bis-MPA derivatives and biocompatible chains derived from 2-[2-(2-methoxyethoxy) ethoxy]acetic acid. Whereas the PAMAM compounds remain cytotoxic, the I-bis-MPA compound exhibits high loading capacity for pDNA and very low toxicity.

    8. Novel Linear Polymers Able to Inhibit Bacterial Quorum Sensing

      Eliana Cavaleiro PhD, Ana Sofia Duarte PhD, Ana Cristina Esteves PhD, António Correia PhD, Michael J. WhitcombePhD, Elena V. Piletska PhD, Sergey A. Piletsky PhD and Iva Chianella PhD

      Article first published online: 28 JAN 2015 | DOI: 10.1002/mabi.201400447

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      The conventional treatment of bacterial infections is based on the administration of antibiotics, which promotes bacterial resistance. We present the development of linear polymers able to disrupt quorum sensing as shown by the reduction of bacterial bioluminescence and biofilm formation. The polymers could be used to control bacterial virulence without promoting resistance, thus constituting a safe alternative to the use of antibiotics.

    9. Polymer Brushes Interfacing Blood as a Route Toward High Performance Blood Contacting Devices

      František Surman, Tomáš Riedel, Michael Bruns, Nina Yu. Kostina, Zdeňka Sedláková and Cesar Rodriguez-Emmenegger

      Article first published online: 21 JAN 2015 | DOI: 10.1002/mabi.201400470

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      Hydrophilic polymer brushes based on HPMA prepared by surface initiated ATRP provide an excellent surface modification to prevent the adhesion of blood components as well as the fouling from whole blood. This surface modification opens new opportunities to improve the performance of blood contacting devices.

    10. Reduction-Sensitive Amphiphilic Triblock Copolymers Self-Assemble Into Stimuli-Responsive Micelles for Drug Delivery

      Smahan Toughraï, Violeta Malinova, Raffaello Masciadri, Sindhu Menon, Pascal Tanner, Cornelia Palivan, Nico Bruns and Wolfgang Meier

      Article first published online: 13 JAN 2015 | DOI: 10.1002/mabi.201400400

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      Polymeric micelles obtained from the self-assembly of redox-sensitive amphiphilic triblock copolymers with a cleavable disulfide bond in the center of the hydrophobic block were investigated as potential drug delivery systems. The degradation products of the micelles are amphiphilic diblock copolymers and should therefore allow for facile clearing from biological systems.

  6. Communications

    1. Cell—Cell Crosslinking by Bio-Molecular Recognition of Heparin-Based Layer-by-Layer Nanofilmsa

      Akihiro Nishiguchi, Michiya Matsusaki and Mitsuru Akashi

      Article first published online: 9 JAN 2015 | DOI: 10.1002/mabi.201400415

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      Bio-molecular recognition between living cells and polymer nanofilms allows for the rapid cellular assembly to form cell aggregates. The aggregates display viscous properties and network structures crosslinked with polymer nanofilms. Specific inter-domain interactions between proteins and glycosaminoglycan cause cell—cell crosslinking. The assembly method supports in vitro 3D-tissue formation.

  7. Full Papers

    1. Stable Emulsions Prepared by Self-assembly of Hyaluronic Acid and Chitosan for Papain Loading

      Donghua Zhao, Wei Wei, Ye Zhu, Jianhua Sun, Qiong Hu and Xiaoya Liu

      Article first published online: 9 JAN 2015 | DOI: 10.1002/mabi.201400486

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      Papain/hyaluronic acid/chitosan complex colloidal particles are obtained through electrostatic interactions. The complexes can be used as biocompatible emulsifiers and novel potential carriers for papain loading. Formation of the complexes in a compact form can protect the enzyme and even cause an increase in enzymatic activity, increasing the applications in the cosmetic, food, and pharmaceuticals industries.

  8. Full Paper

    1. Tuning the Buffering Capacity of Polyethylenimine with Glycerol Molecules for Efficient Gene Delivery: Staying In or Out of the Endosomes

      Bijay Singh, Sushila Maharjan, Tae-Eun Park, Tao Jiang, Sang-Kee Kang, Yun-Jaie Choi and Chong-Su Cho

      Article first published online: 8 JAN 2015 | DOI: 10.1002/mabi.201400463

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      Non-viral gene delivery vehicles are developed by tuning the buffering capacity of PEI with glycerol molecules. Mechanistic studies reveal that the glycerol-crosslinked PEI appears to trigger macropinocytosis for higher cellular uptake, in addition to clathrin- and caveolae-mediated endocytosis. Independent of endocytic pathway, the endosomal escape of polyplexes depends on the buffering capacity of the polymers, elucidating the intracellular fate of polyplexes.

  9. Full Papers

    1. The Application of LbL-Microcarriers for the Treatment of Chronic Inflammation: Monitoring the Impact of LbL-Microcarriers on Cell Viability

      Mandy Fichtner, Claudia Claus, Jacqueline Lessig-Owlanj, Jürgen Arnhold and Uta Reibetanz

      Article first published online: 7 JAN 2015 | DOI: 10.1002/mabi.201400405

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      Layer-by-Layer (LbL) coated microparticles and microcapsules represent a unique drug delivery system due to their modular, as well as multifunctional characteristic. A promising application is the treatment of chronic inflammation. LbL-microcarriers transporting anti-inflammatory substances into the affected tissue provide to overcome several drawbacks of conventional therapy. However, microcarrier-induced loss of cell viability has to be strongly avoided.

  10. Communications

    1. Tough, In-Situ Thermogelling, Injectable Hydrogels for Biomedical Applications

      Ghulam Jalani, Derek H. Rosenzweig, Georges Makhoul, Sherif Abdalla, Renzo Cecere, Fiorenzo Vetrone, Lisbet Haglund and Marta Cerruti

      Article first published online: 3 JAN 2015 | DOI: 10.1002/mabi.201400406

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      Homogenous, in-situ thermogelling, highly tough, non-toxic injectable hydrogels from chitosan and hyaluronic acid co-crosslinked with β-glycerophophate and genipin are produced. The co-crosslinked gels form faster than genipin only crosslinked gels and are 2.5 times stronger than ionically crosslinked gels. Homogenously dispersed cells express enhanced amount of collagen II, showing great potential of these gels for cartilage tissue engineering.

  11. Full Papers

    1. Agarose-Assisted Micro-Contact Printing for High-Quality Biomolecular Micro-Patterns

      Min Jee Jang and Yoonkey Nam

      Article first published online: 3 JAN 2015 | DOI: 10.1002/mabi.201400407

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      We present a novel surface modification method for a PDMS stamp with agarose hydrogel in order to enhance the micro-contact printing of biomolecules. A thin film of agarose was formed on the stamp by a simple dip-coating procedure, which dramatically enhanced the transferability of ink molecules to the substrate and the uniformity of printed patterns.

    2. Effects of Permeability and Living Space on Cell Fate and Neo-Tissue Development in Hydrogel-Based Scaffolds: A Study With Cartilaginous Model

      Changjiang Fan and Dong-An Wang

      Article first published online: 2 JAN 2015 | DOI: 10.1002/mabi.201400453

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      The effects of permeability and living space of hydrogel scaffolds on cell fate and neo-tissue development are studied, respectively. The better permeability of hydrogel facilitates cell survival and rapid proliferation. The presence of macro-porous living spaces favors the formation of larger cell clusters, which significantly enhance ECM deposition and eventually induce formation of neo-tissue.

    3. Phosphorylcholine-Modified Chitosan Films as Effective Promoters of Cell Aggregation: Correlation Between the Films Properties and Cellular Response

      Baowen Qi, Piotr Kujawa, Sayaka Toita, Grégory Beaune and Françoise M. Winnik

      Article first published online: 2 JAN 2015 | DOI: 10.1002/mabi.201400439

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      Polymer substrates composed of an adhesive part, Chitosan, and of an antifouling part, phosphorylcholine, are prepared and characterized. Surface topography, zeta potential, and protein adsorption are investigated as a function of phosphorylcholine content. These results are related to the capacity of the substrates containing most of phosphorylcholine to promote the aggregation of cells.

  12. Communications

    1. PEG-Poly(l-alanine) Thermogel As a 3D Scaffold of Bone-Marrow-Derived Mesenchymal Stem Cells

      Min Hee Park, Hyo Jung Moon, Jin Hye Park, Usha Pramod Shinde, Du Young Ko and Byeongmoon Jeong

      Article first published online: 16 DEC 2014 | DOI: 10.1002/mabi.201400426

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      Polypeptide thermogel prepared by increasing the temperature of cell suspended polymer aqueous solution to 37 °C provides an excellent 3D culture matrix of bone-marrow-derived mesenchymal stem cells. Both in vitro and in vivo studies on three-dimensional culture of the stem cells prove the preferential chondrogenic differentiation of the stem cells in the in situ formed hydrogel.

  13. Full Papers

    1. Enhanced Efficacy of Pluronic Copolymer Micelle Encapsulated SCR7 against Cancer Cell Proliferation

      Franklin John, Jinu George, Supriya V. Vartak, Mrinal Srivastava, P. A. Hassan, V. K. Aswal, Subhas. S. Karki and Sathees C. Raghavan

      Article first published online: 16 DEC 2014 | DOI: 10.1002/mabi.201400480

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      Encapsulation of 5,6-bis-(benzylideneamino)-2-mercaptopyrimidin-4-ol (SCR7) in Pluronic P123 copolymer resulted in accumulation of DNA breaks within the cells, leading to cell cycle arrest at G1 phase and activation of apoptosis. Micellar encapsulation rendered the molecule about 5 times more effective than the parenteral form.

  14. Reviews

    1. Self-assembled Monolayers and Nanocomposite Hydrogels of Functional Nanomaterials for Tissue Engineering Applications

      Nermin Seda Kehr, Seda Atay and Bahar Ergün

      Article first published online: 16 DEC 2014 | DOI: 10.1002/mabi.201400363

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      Self-assembled monolayers (SAMs) and nanocomposite (NC) hydrogels of functional nanomaterials (NMs) have gained high interest as biomaterials to mimic natural extracellular matrix and to control cell/material interactions. This paper highlights some of the recent reports on the use of SAMs and NC hydrogels of functional NMs as biomaterials particularly in cell culturing applications.

  15. Full Papers

    1. Semiconductor Polymer Dots Induce Proliferation in Human Gastric Mucosal and Adenocarcinoma Cells

      Shouying Li, Jia Chen, Geng Chen, Qiong Li, Kai Sun, Zhen Yuan, Weiping Qin, Hong Xu and Changfeng Wu

      Article first published online: 12 DEC 2014 | DOI: 10.1002/mabi.201400428

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      Highly fluorescent polymer dots induce apparent proliferation in human gastric mucosal and adenocarcinoma cells, as validated by MTT viability studies, cell cycle analysis, ROS generation, and MMP changes. The Pdot-induced cell proliferation represents a distinctive finding as most of other nanoparticles interacted with cells typically cause cell damage and inhibit cell growth.

    2. Synthesis and Functions of Well-defined Polymer-drug Conjugates as Efficient Nanocarriers for Intravesical Chemotherapy of Bladder Cancer

      Qingsong Yu, Jiajing Zhang, Guan Zhang and Zhihua Gan

      Article first published online: 12 DEC 2014 | DOI: 10.1002/mabi.201400416

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      Water-soluble polymer-doxorubicin conjugates based on poly(ethylene glycol)-b-poly(N-2-(hydroxypropyl methacrylamide)) for controlled release of anti-cancer drugs under mild acidic conditions are synthesized by RAFT polymerization and post-polymerization technique. With further indroducing targeting moiety folate, the conjugates show enhanced cytotoxicity against T24 cells.

    3. Peptide-Directed Self-Assembly of Functionalized Polymeric Nanoparticles. Part II: Effects of Nanoparticle Composition on Assembly Behavior and Multiple Drug Loading Ability

      Xu Xiang, Xiaochu Ding, Trevor Moser, Qi Gao, Tolou Shokuhfar and Patricia A. Heiden

      Article first published online: 4 DEC 2014 | DOI: 10.1002/mabi.201400438

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      A novel nanoparticle scaffolding system is demonstrated using controlled-release, drug-loaded nanoparticles that yield ordered fibers and scaffolds using ionic complementary peptide-directed self-assembly. The scaffolding system possesses the advantages of individually controlling the release of each loaded drug and the ability to control their assembly sequence.

  16. Feature Articles

    1. Recent Advances in Nanoscale Bioinspired Materials

      Melik C. Demirel, Murat Cetinkaya, Abdon Pena-Francesch and Huihun Jung

      Article first published online: 4 DEC 2014 | DOI: 10.1002/mabi.201400324

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      This feature article highlights structural and functional characteristics of bio-derived materials, which will expedite the design, fabrication, and synthesis of eco-friendly, recyclable, advanced nano-materials and devices. Recent Advances in Nanoscale Bioinspired Materials.

  17. Full Papers

    1. Engineering a Freestanding Biomimetic Cardiac Patch Using Biodegradable Poly(lactic-co-glycolic acid) (PLGA) and Human Embryonic Stem Cell-derived Ventricular Cardiomyocytes (hESC-VCMs)

      Yin Chen, Junping Wang, Bo Shen, Camie W. Y. Chan, Chaoyi Wang, Yihua Zhao, Ho N. Chan, Qian Tian, Yangfan Chen, Chunlei Yao, I-Ming Hsing, Ronald A. Li and Hongkai Wu

      Article first published online: 25 NOV 2014 | DOI: 10.1002/mabi.201400448

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      A facile method for engineering a freestanding biomimetic cardiac patch using biodegradable poly(lactic-co-glycolic acid) (PLGA) and stem cell-derived cardiomyocytes is presented. The biomimetic cardiac patch fabricated with microgrooved PLGA film recapitulates the anisotropic electrophysiological feature of native cardiac tissue and is believed to be applicable in the treatment of myocardial infarction.

    2. Multiphase Protein Microgels

      Ulyana Shimanovich, Yang Song, Jasna Brujic, Ho Cheung Shum and Tuomas P. J. Knowles

      Article first published online: 19 NOV 2014 | DOI: 10.1002/mabi.201400366

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      We show that the characteristics of aqueous two-phase systems can be exploited in combination with protein self-assembly for controlling aggregation and structuring of fibrillar proteins into 3D core–shell microgels.

    3. A Cationic Poly(2-oxazoline) with High In Vitro Transfection Efficiency Identified by a Library Approach

      Alexandra C. Rinkenauer, Lutz Tauhardt, Felix Wendler, Kristian Kempe, Michael Gottschaldt, Anja Traeger and Ulrich S. Schubert

      Article first published online: 18 NOV 2014 | DOI: 10.1002/mabi.201400334

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      An 18-membered library of cationic poly(2-oxazoline)-based copolymers has been prepared. The influence of different polymer parameters, namely the polymer side chain hydrophobicity and the type and content of amino groups, on the pDNA condensation, the transfection efficiency, the cytotoxicity, and cellular membrane interaction as well as the size, charge, and stability of the polyplexes was studied and first structure–property relationships elucidated.

    4. Protein Compatibility of Selected Cross-linking Reactions for Hydrogels

      Nadine Hammer, Ferdinand P. Brandl, Susanne Kirchhof, Viktoria Messmann and Achim M. Goepferich

      Article first published online: 14 NOV 2014 | DOI: 10.1002/mabi.201400379

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      Despite years of research, finding cross-linking reactions for hydrogels that are compatible with incorporated proteins is still challenging. The authors, therefore, investigate side reactions between a model protein and methoxypolyethylene glycol functionalized with maleimide, vinyl sulfone, acrylamide, thiol, and furyl moieties. The extent of protein modification was found to depend on the cross-linking agent and the pH during incubation.

    5. Targeted Delivery of Vaccine to Dendritic Cells by Chitosan Nanoparticles Conjugated with a Targeting Peptide Ligand Selected by Phage Display Technique

      Su-Na Jung, Sang-Kee Kang, Guen-Hye Yeo, Hai-Ying Li, Tao Jiang, Jae-Woon Nah, Jin-Duck Bok, Chong-Su Cho and Yun-Jaie Choi

      Article first published online: 13 NOV 2014 | DOI: 10.1002/mabi.201400352

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      Delivering antigen to dendritic cells (DCs) is a promising strategy for enhancing vaccine efficacy. A novel DC-targeting peptide ligand is isolated by phage display technique. DC-targeting peptide conjugated chitosan nanoparticles (TPC-NPs) are designed and evaluated for their targeting efficiency to DCs. Furthermore, immunization of mice with antigen-carrying TPC-NPs induces strong and balanced immune responses, demonstrating that TPC-NPs can further improve the immunogenicity of vaccine.

    6. Micellization of Antineoplastic Agent to Significantly Upregulate Efficacy and Security

      Chen Li, Weiguo Xu, Jianxun Ding, Ying Zhang, Jincheng Wang, Xiuli Zhuang and Xuesi Chen

      Article first published online: 7 NOV 2014 | DOI: 10.1002/mabi.201400356

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      Doxorubicin-incorporated micelle is prepared by noncovalent interaction-assissed nanoprecipitation of drug and methoxy poly(ethylene glycol)-block-oligo(D,L-leucine). The loading micelle exhibits enhanced cellular interalization and antiproliferative activity in vitro, improved intratumoral distribution, and upregulated antitumor efficacy and security in vivo, which all indicate its great potential for smart delivery of antineoplastic agents.

    7. Diblock Copolymers of Polyethylene Glycol and a Polymethacrylamide with Side-Chains Containing Twin Ortho Ester Rings: Synthesis, Characterization, and Evaluation as Potential pH-Responsive Micelles

      Xiaojing Zhou, Shi Luo, Rupei Tang, Rui Wang and Jun Wang

      Article first published online: 5 NOV 2014 | DOI: 10.1002/mabi.201400395

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      Block copolymers, PEG-b-PMEA, bearing twin five-membered ortho ester pendants, formed micelles with pH-responsive solubility as a result of accelerated release of payload. The micelles were compatible with cells, and paclitaxel-loaded micelles killed cancer cells with potency equivalent to free drug, suggesting potential use as effective nano-carriers for anticancer drugs without toxic side-effects.

    8. Effect of Amphiphilic PCL–PEG Nano-Micelles on HepG2 Cell Migration

      Yang Shen, Mengtian Leng, Hongchi Yu, Qin Zhang, Xianglin Luo, Hans Gregersen, Guixue Wang and Xiaoheng Liu

      Article first published online: 4 NOV 2014 | DOI: 10.1002/mabi.201400376

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      Nano-sized polymer micelles attract attentions to their biocompatibility and biodegradability in antitumor therapy. However, few investigations focus on exploring the effect of internalized nano-micelles on the cancer cells migration. We demonstrated that amphiphilic PCL–PEG NPs-induced cell migration depended on integrin-FAK-Rho GTPases pathway, which expected to evoke more attentions in the design of drug delivery systems based on polymer micelles.

    9. Degradable Glycine-Based Photo-Polymerizable Polyphosphazenes for Use as Scaffolds for Tissue Regeneration

      Sandra Rothemund, Tamara B. Aigner, Aitziber Iturmendi, Maria Rigau, Branislav Husár, Florian Hildner, Eleni Oberbauer, Martina Prambauer, Gbenga Olawale, Reinhard Forstner, Robert Liska, Klaus R. Schröder, Oliver Brüggemann and Ian Teasdale

      Article first published online: 30 OCT 2014 | DOI: 10.1002/mabi.201400390

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      Hydrolytically degradable, porous matrices are prepared via a mild and simple photopolymerization of functionalized poly(organo)phosphazenes. The modular system allows for facile surface functionalization and property tuning. The novel materials are biocompatible and have tunable degradation rates to non-toxic small molecules and thus are promising for use as synthetic scaffolds for tissue regeneration.

    10. Substrate-induced PC12 Cell Differentiation Without Filopodial, Lamellipodial Activity or NGF Stimulation

      Guillaume Lamour, Sylvie Souès and Ahmed Hamraoui

      Article first published online: 28 OCT 2014 | DOI: 10.1002/mabi.201400323

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      PC12 cells are driven to extend neurites through cell-substrate interactions only, that is, without nerve growth factor (NGF) stimulation. Actin polymerization is destabilized by treating the cells with cytochalasin. As a result the cells are deprived of both filopodia and lamellipodia. However, they extend neurites continuously. This shows that neither filopodia nor lamellipodia are required to trigger substrate-induced neuronal differentiation of PC12 cells.

    11. Poly(anhydride-ester) and Poly(N-vinyl-2-pyrrolidone) Blends: Salicylic Acid-Releasing Blends with Hydrogel-Like Properties that Reduce Inflammation

      Michelle A. Ouimet, Renata Fogaça, Sabrina S. Snyder, Sameer Sathaye, Luiz H. Catalani, Darrin J. Pochan and Kathryn E. Uhrich

      Article first published online: 21 OCT 2014 | DOI: 10.1002/mabi.201400238

      Thumbnail image of graphical abstract

      Hydrogels-like polymer blends that combine the biocompatibility and hydrophilicity of PVP with controlled release of salicylic acid to reduce inflammation are presented. These copolymer blends substantially reduce the inflammatory cytokine, TNF-α, in vitro and indicate no cytotoxic or anti-proliferative effects. Based upon the unique combination, these blended polymer films show great potential for wound healing.

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