Macromolecular Bioscience

Cover image for Vol. 15 Issue 9

Early View (Online Version of Record published before inclusion in an issue)

Editor-in-Chief: Kirsten Severing, Editor: Anne Pfisterer

Online ISSN: 1616-5195

Associated Title(s): Advanced Healthcare Materials, Macromolecular Chemistry and Physics, Macromolecular Materials and Engineering, Macromolecular Rapid Communications

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  1. Full Papers

    1. Regional Morphology and Transport of PAMAM Dendrimers Across Isolated Rat Intestinal Tissue

      Dallin Hubbard, Tanner Bond and Hamidreza Ghandehari

      Article first published online: 2 SEP 2015 | DOI: 10.1002/mabi.201500225

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      PAMAM dendrimers have been observed to traverse the intestinal epithelium as a macromolecule. Herein is established the regional dependence of PAMAM dendrimers in isolated tissue models using rat intestinal epithelium. The histology of isolated rat intestinal tissues in the presence of PAMAM dendrimers is established.

    2. Investigation of Antifouling Properties of Surfaces Featuring Zwitterionic α-Aminophosphonic Acid Moieties

      Natalie Wagner, Phyllis Zimmermann, Peter Heisig, Franziska Klitsche, Wolfgang Maison and Patrick Theato

      Article first published online: 2 SEP 2015 | DOI: 10.1002/mabi.201500196

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      Zwitterionic α-aminophosphonic acid surfaces are prepared by successful surface-Kabachnik–Fields reaction of an aldehyde-functionalized surface with amines and dialkyl phosphonates and subsequent deprotection. The obtained zwitterionic surfaces exhibit antifouling properties.

    3. Protein-Containing Multilayer Capsules by Templating on Mesoporous CaCO3 Particles: POST- and PRE-Loading Approaches

      Nadezhda G. Balabushevich, Anna V. Lopez de Guerenu, Natalia A. Feoktistova, Andre G. Skirtach and Dmitry Volodkin

      Article first published online: 26 AUG 2015 | DOI: 10.1002/mabi.201500243

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      Protein encapsulation into polymer-based multilayer capsules is performed by templating on sacrificial porous CaCO3 microparticles. We focus on protein encapsulation by considering two approaches: POST-loading into performed capsules and PRE-loading into the carbonate microparticles by adsorption or co-synthesis. Protein content and its activity strongly depend on the encapsulation approach to be chosen for successful encapsulation.

    4. Highly Fluorescent Gene Carrier Based on Ag–Au Alloy Nanoclusters

      Ping Wang, Lin Lin, Zhaopei Guo, Jie Chen, Huayu Tian, Xuesi Chen and Hua Yang

      Article first published online: 19 AUG 2015 | DOI: 10.1002/mabi.201500235

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      This research has successfully prepared a highly fluorescent gene carrier Ag–Au–PEI, which shows much higher quantum yield than most of the reported gold nanocluster-based quantum dots. The high fluorescent Ag–Au–PEI conjugates not only exhibit remarkable high gene transfection efficiencies and relatively low cytotoxicity in vitro but also possess bioimaging capacity in vivo.

    5. Enhanced Cytoplasmic Delivery of RAGE siRNA Using Bioreducible Polyethylenimine-based Nanocarriers for Myocardial Gene Therapy

      Min Jung Yang, Sook Hee Ku, Dongkyu Kim, Won Jong Kim, Hyejung Mok, Sun Hwa Kim and Ick Chan Kwon

      Article first published online: 19 AUG 2015 | DOI: 10.1002/mabi.201500213

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      Bioreducible polyethylenimine (rPEI) is synthesized from low molecular weight PEI via cleavable disulfide linkages to achieve high stability, high transfection efficiency, high cytoplasmic siRNA release, and low cytotoxicity. rPEI/siRAGE polyplex can reduce RAGE expression in ischemic/reperfused myocardium, thereby leading to attenuation of myocardial infarction.

    6. Self-Assembly of a Multifunctional Lipid With Core–Shell Dendrimer DNA Nanoparticles Enhanced Efficient Gene Delivery at Low Charge Ratios into RPE Cells

      Da Sun, Hiroshi Maeno, Maneesh Gujrati, Rebecca Schur, Akiko Maeda, Tadao Maeda, Krzysztof Palczewski and Zheng-Rong Lu

      Article first published online: 13 AUG 2015 | DOI: 10.1002/mabi.201500192

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      A hybrid gene delivery system comprised of a multifunctional lipid ECO and a core–shell dendrimer mediates high gene transfection efficiency at a low charge ratio in RPE cells. The non-viral delivery system is effective for in vivo gene transfection in the RPE cells after subretinal injection and promising in treating genetic disorders of the eye.

    7. Asymmetric AB3 Miktoarm Star Polymers: Synthesis, Self-Assembly, and Study of Micelle Stability Using AF4 for Efficient Drug Delivery

      Alexandre Moquin, Anjali Sharma, Yiming Cui, Anthony Lau, Dusica Maysinger and Ashok Kakkar

      Article first published online: 11 AUG 2015 | DOI: 10.1002/mabi.201500186

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      AB3 (PCL–PEG3) miktoarm polymers form micelles and incorporate large amount of hydrophobic drug (curcumin). Size distribution of the micelles is narrow as determined by AF4 fractionation. Curcumin-loaded AB3 micelles exert significant cell loss in glioblastoma (GBM) spheroids.

    8. Long-Term Controlled Protein Release from Poly(Ethylene Glycol) Hydrogels by Modulating Mesh Size and Degradation

      Xinming Tong, Soah Lee, Layla Bararpour and Fan Yang

      Article first published online: 11 AUG 2015 | DOI: 10.1002/mabi.201500245

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      A PEG-based hydrogel platform is developed to allow long-term controlled protein release. No protein is released from non-degradable PEG hydrogels, confirming efficient loading via physical entrapment. Degradation of hydrogels increasing swelling ratio and mesh size enables sustained protein release. bFGF released from hydrogel over 35 d continues to stimulate cell proliferation comparable to fresh bFGF.

    9. Efficient pDNA Delivery Using Cationic 2-Hydroxypropyl-β-Cyclodextrin Pluronic-Based Polyrotaxanes

      Vivek Badwaik, Yawo Mondjinou, Aditya Kulkarni, Linjia Liu, Asher Demoret and David H. Thompson

      Article first published online: 10 AUG 2015 | DOI: 10.1002/mabi.201500220

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      Dried α, ω-bis-tris (2-aminoethyl) amine Pluronic triblock copolymer (0.08 mmol) and 2-hydroxypropyl-β-cyclodextrin (using the ratio of CD:PPG unit = 1:2 for each type of Pluronic) were dissolved (or suspended) in 60 mL hexane and the mixtures were vortexed before vigorously stirring for 2 h. The mixtures were then gently stirred for 72 h at 20 °C and cholesteryl chloroformate (12 equiv) was used to cap the polyrotaxanes before purification and lyophilization.

    10. Cellular Response to Linear and Branched Poly(acrylic acid)

      Elizabeth G. Whitty, Alison R. Maniego, Sharon A. Bentwitch, Yohann Guillaneuf, Mark R. Jones, Marianne Gaborieau and Patrice Castignolles

      Article first published online: 10 AUG 2015 | DOI: 10.1002/mabi.201500153

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      The cytotoxicity and intracellular effects of poly(acrylic acid)/poly(sodium acrylate) (PNaA) are investigated. This pH-responsive polymer has potential in anticancer drug delivery. Capillary electrophoresis demonstrates interactions of PNaA with serum proteins. The MTT assay is used to assess the cytotoxicity of PNaA with different branching architectures for L1210 progenitor leukemia cells and L6 myoblast cells.

    11. Nontoxic, Hydrophilic Cationic Polymers—Identified as Class of Antimicrobial Polymers

      Arne Strassburg, Frauke Kracke, Julia Wenners, Anna Jemeljanova, Jannis Kuepper, Hanne Petersen and Joerg C. Tiller

      Article first published online: 4 AUG 2015 | DOI: 10.1002/mabi.201500207

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      Antimicrobially active and nontoxic hydrophilic polycations based on 1,4-dibromo-2-butene and tetramethyl-1,3-propanediamine named PBI are an alternative to toxic biocides and amphiphilic polycations. Investigations on PBIs with defined molecular weight (Mn) and tailored end groups revealed that there is a dependence of antimicrobial activity and selectivity on Mn and nature of the end groups.

  2. Communications

    1. Star-Shaped, Biodegradable, and Elastomeric PLLA-PEG-POSS Hybrid Membrane With Biomineralization Activity for Guiding Bone Tissue Regeneration

      Meihua Xie, Juan Ge, Bo Lei, Qian Zhang, Xiaofeng Chen and Peter X. Ma

      Article first published online: 4 AUG 2015 | DOI: 10.1002/mabi.201500237

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      Novel degradable star-shaped poly-L-lactide-poly (ethylene glycol)-polyhedral oligomeric silsesquioxane (SPPS) hybrid implants with highly controlled mechanical properties, and with elastomeric behavior, biomineralization activity, and cell biocompatibility are fabricated successfully by a facile photo-crosslinking method.

  3. Feature Articles

    1. Biofunctional Materials Based on Amino Cellulose Derivatives – A Nanobiotechnological Concept

      Thomas Heinze, Melanie Siebert, Peter Berlin and Andreas Koschella

      Article first published online: 30 JUL 2015 | DOI: 10.1002/mabi.201500184

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      Amino cellulose derivatives simply available by nucleophilic displacement reactions of tosyl cellulose with a broad variety of amines possess an extraordinary ability for self-assembling. Nanocomposites and monolayers may be formed on various substrates. For example, the modified surfaces are efficient carriers for (bio) functional molecules yielding efficient biosensors.

  4. Communications

    1. Anti-Resorptive Functions of Poly(ethylene sodium phosphate) on Human Osteoclasts

      Sujit Kootala, Masahiro Tokunaga, Jöns Hilborn and Yasuhiko Iwasaki

      Article first published online: 29 JUL 2015 | DOI: 10.1002/mabi.201500166

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      The effect of poly(ethylene sodium phosphate) (PEP · Na) on human osteoclasts is studied. PEP · Na exhibits excellent biocompatibility with osteoblasts, whereas the polymer effectively reduces the adhesion of osteoclasts adhered on bone slices. This is the first report to describe the possibility of using polyphosphoesters for selective inhibition of osteoclast adhesion and function.

    2. Anti-Bioadhesive Coating Based on Easy to Make Pseudozwitterionic RAFT Block Copolymers for Blood-Contacting Applications

      Sabrina Nehache, Chin-Cheng Yeh, Mona Semsarilar, André Deratani, Yung Chang and Damien Quemener

      Article first published online: 29 JUL 2015 | DOI: 10.1002/mabi.201500185

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      Well-defined pseudozwitterionic diblock copolymers are synthesized using RAFT chemistry. These charged polymers are then used to prepare anti-bioadhesion surfaces that could be used in blood-contact devices. It is demonstrated that such coatings prevent blood protein adsorption to the polymer modified surfaces.

  5. Full Papers

    1. Bioreducible Poly-l-Lysine–Poly[HPMA] Block Copolymers Obtained by RAFT-Polymerization as Efficient Polyplex-Transfection Reagents

      Kristof Tappertzhofen, Simone Beck, Evelyn Montermann, David Huesmann, Matthias Barz, Kaloian Koynov, Matthias Bros and Rudolf Zentel

      Article first published online: 29 JUL 2015 | DOI: 10.1002/mabi.201500212

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      Polylysine-S-S-b-poly[N-(2-hydroxypropyl) methacrylamide] (p[HPMA]) copolymers with a bioreducible disulfide group can be obtained by RAFT polymerization of a macromolecular disulfide chain transfer agent (CTA). Polyplex formation with pDNA leads to increased transfection efficiencies compared to non disulfide containing block copolymers.

    2. N-(2-Hydroxypropyl)methacrylamide Copolymer–Drug Conjugates for Combination Chemotherapy of Acute Myeloid Leukemia

      Rui Zhang, Jiyuan Yang, Yan Zhou, Paul J. Shami and Jindřich Kopeček

      Article first published online: 29 JUL 2015 | DOI: 10.1002/mabi.201500193

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      Four different drugs, including cytarabine, daunorubicin, GDC-0980, and JS-K, are used for two-drug combination treatment against acute myeloid leukemia. Combining cytarabine with GDC-0980 shows the strongest synergistism. N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-cytarabine/GDC-0980 conjugates are synthesized. They also hve potent cytotoxicity and strong synergstism in combination.

    3. Exploring the Properties of Genetically Engineered Silk-Elastin-Like Protein Films

      Raul Machado, André da Costa, Vitor Sencadas, Ana Margarida Pereira, Tony Collins, José Carlos Rodríguez-Cabello, Senentxu Lanceros-Méndez and Margarida Casal

      Article first published online: 27 JUL 2015 | DOI: 10.1002/mabi.201500132

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      Free standing films of a genetically engineered silk-elastin-like protein are prepared by solvent casting using water and formic acid as solvents. The films are exposed to methanol-saturated air to induce the formation of aggregated β-strands and extensively characterized for their properties. Results indicate that the films are electrically insulating with excellent optical clarity and mechanical properties, depending of the post-treatment and glycerol content.

  6. Communications

    1. Orthotopic Osteogenecity Enhanced by a Porous Gelatin Sponge in a Critical-Sized Rat Calvaria Defect

      Naofumi Kanda, Takahisa Anada, Takuto Handa, Kazuhito Kobayashi, Yushi Ezoe, Tetsu Takahashi and Osamu Suzuki

      Article first published online: 22 JUL 2015 | DOI: 10.1002/mabi.201500191

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      Preparation of gelatin disks for bone regeneration: this study is designed to investigate whether a gelatin sponge with a specific porous structure exhibits orthotopic osteogenecity in critical-sized rat calvarial defects. The results provide the findings that a Gel sponge is capable of inducing new bone formation orthotopically, if the scaffold has a specific porous structure.

  7. Full Papers

    1. Study on Self-Assembled Well-Defined PEG Graft Copolymers as Efficient Drug-Loaded Nanoparticles for Anti-Inflammatory Therapy

      Paulina Maksym-Bębenek and Dorota Neugebauer

      Article first published online: 22 JUL 2015 | DOI: 10.1002/mabi.201500189

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      Hydroxy-functional PEG graft polymethacrylates are designed as functional nanocarriers. The cumulative influence of hydrophilic/hydrophobic balance, degree of grafting, length of side chains and backbone, on micellization, drug loading and release profiles is demonstrated.

    2. Investigation of Functionalized Poly(N,N-dimethylacrylamide)-block-polystyrene Nanoparticles As Novel Drug Delivery System to Overcome the Blood–Brain Barrier In Vitro

      Maria Gregori, Daniela Bertani, Emanuela Cazzaniga, Antonina Orlando, Michele Mauri, Alberto Bianchi, Francesca Re, Silvia Sesana, Stefania Minniti, Maura Francolini, Alfredo Cagnotto, Mario Salmona, Luca Nardo, Domenico Salerno, Francesco Mantegazza, Massimo Masserini and Roberto Simonutti

      Article first published online: 21 JUL 2015 | DOI: 10.1002/mabi.201500172

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      In the search of novel drug carriers for brain drug delivery, poly(N,N-dimethylacrylamide)-b-polystyrene nanoparticles (NP) have been functionalized with a modified fragment of Apolipoprotein E (mApoE). These NP show to be uptaken and to overcome a BBB in vitro cellular model. The cytotoxicity of NP is also investigated on different cell lines, showing high biocompatibility.

  8. Communications

    1. Microfluidic Production of Alginate Hydrogel Particles for Antibody Encapsulation and Release

      Linas Mazutis, Remigijus Vasiliauskas and David A. Weitz

      Article first published online: 21 JUL 2015 | DOI: 10.1002/mabi.201500226

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      Using a microfluidic device with a characteristic flow-focusing junction that facilitates the break-up of viscous fluids into monodisperse droplets the authors produce alginate microparticles of unique biconcave shape resembling the red blood cells. They loaded alginate particles with fluorescently labeled antibodies and evaluated their release over time in different aqueous buffers and tested the biocompatibility in the whole blood sample.

    2. Synthesis and Characterization of pH-Responsive Copolypeptides Vesicles for siRNA and Chemotherapeutic Drug Co-Delivery

      Zhanrong Li, Jingguo Li, Jinsheng Huang, Junjie Zhang, Du Cheng and Xintao Shuai

      Article first published online: 17 JUL 2015 | DOI: 10.1002/mabi.201500161

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      A novel pH-sensitive polymeric vesicles based on diblock copolypeptides for anti-cancer drugs and siRNA co-delivery are described. The vesicles are stable in aqueous solutions at neutral pH and disassemble in acidic pHs. The vesicles can co-deliver DOX and siRNA inside the same cell and exhibit a pH-sensitive drug release behavior.

  9. Full Papers

    1. Neurogenesis Is Induced by Electrical Stimulation of Human Mesenchymal Stem Cells Co-Cultured With Mature Neuronal Cells

      Sang Jun Park, Ji Sun Park, Han Na Yang, Se Won Yi, Chun-Ho Kim and Keun-Hong Park

      Article first published online: 17 JUL 2015 | DOI: 10.1002/mabi.201500115

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      The effects of both electrical stimulation and co-culturing hMSCs with neuronal cells are tested. The hMSCs co-cultured with mature neuronal cells and stimulated with electrical shock show the greatest level of neurite outgrowth (>150 µm) and smaller cell body sizes. The neuronal differentiation of the adhered hMSCs onto glass cover slips with gold nanoparticles are also evaluated for their specific genes and proteins.

    2. Crosslinked Polypropylenimine Dendrimers With Bioreducible Linkages for Gene Delivery Systems and Their Reductive Degradation Behaviors

      Gyeong Jin Lee, Kitae Ryu, Kyunghwan Kim, Ji-yeong Choi and Tae-il Kim

      Article first published online: 16 JUL 2015 | DOI: 10.1002/mabi.201500141

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      Crosslinked bioreducible polypropylenimine dendrimers (PPI–CBAs) showed high stability even in reducing condition probably due to the re-crosslinking. It is thought that this may lead to additional consumption of glutathione and the following increase of intracellular reactive oxygen species according to the concentration and crosslinking degree of PPI–CBAs, finally inducing cytotoxicity by oxidative stress.

    3. Fluorescent Phosphorus Dendrimer as a Spectral Nanosensor for Macrophage Polarization and Fate Tracking in Spinal Cord Injury

      Antos Shakhbazau, Manoj Mishra, Tak-Ho Chu, Craig Brideau, Karen Cummins, Shigeki Tsutsui, Dzmitry Shcharbin, Jean-Pierre Majoral, Serge Mignani, Mireille Blanchard-Desce, Maria Bryszewska, V. Wee Yong, Peter K. Stys and Jan van Minnen

      Article first published online: 14 JUL 2015 | DOI: 10.1002/mabi.201500150

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      Fluorescent phosphorus dendrimer is readily internalized and maintained by bone marrow-derived macrophages, making it a useful cytoplasmic tracer. Sensitivity of dendrimer emission profile to the macrophage physiological status generates distinct spectral phenotypes, a feature which may offer new strategies for targeted release of therapeutic cargos, and assist in tracing macrophage plasticity in longitudinal studies in vitro and in vivo.

    4. Acid-Sensitive Nanogels for Synergistic Chemo-Photodynamic Therapy

      Xiaofei Chen, Xuemei Yao, Li Chen and Xuesi Chen

      Article first published online: 14 JUL 2015 | DOI: 10.1002/mabi.201500180

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      A novel kind of intelligent nanogels is designed, which can spatiotemporally control the release of drug (doxorubicin) and photosensitizers (porphyrins) to combine chemo-therapy and photodynamic therapy..

    5. Targeted Imaging and Chemo-Phototherapy of Brain Cancer by a Multifunctional Drug Delivery System

      Yongwei Hao, Lei Wang, Yalin Zhao, Dehui Meng, Dong Li, Haixia Li, Bingxiang Zhang, Jinjin Shi, Hongling Zhang, Zhenzhong Zhang and Yun Zhang

      Article first published online: 14 JUL 2015 | DOI: 10.1002/mabi.201500091

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      Multifunctional poly (lactide-co-glycolic acid) nanoparticles are developed by a modified oil in water single-emulsion solvent evaporation method. They could simultaneously deliver indocyanine green (ICG) and docetaxel (DTX) to the brain by surface decoration with the brain-targeting peptide angiopep-2 to achieve combined chemo-phototherapy for glioma under near-infrared (NIR) imaging. Under the 808 nm laser irradiation, the glioma growth of the nude mice bearing U87MG glioma is efficiently inhibited.

    6. Visualizing the Inner Architecture of Poly(ϵ-caprolactone)-Based Biomaterials and Its Impact on Performance Optimization

      Adam J. P. Bauer, Yitian Wu, Jianzhao Liu and Bingbing Li

      Article first published online: 7 JUL 2015 | DOI: 10.1002/mabi.201500175

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      This study demonstrates the superior degradation selectivity of Candida antarctica lipase B (CALB) enzyme; when used at low concentrations, it preferentially breaks down the amorphous chains of poly(ϵ-caprolactone) (PCL) prior to its crystalline chains. Top-down dissection using this enzyme is performed on several PCL-based model systems. The spatial distribution of the amorphous compartments are precisely mapped out, which otherwise cannot be achieved.

    7. A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting

      Fabio Salvatore Palumbo, Antonella Bavuso Volpe, Flavia Bongiovì, Giovanna Pitarresi and Gaetano Giammona

      Article first published online: 1 JUL 2015 | DOI: 10.1002/mabi.201500129

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      New cationic HA derivatives (HA-EDA-pDEAEMA), synthesized by atom transfer radical polymerization (ATRP) are able to complex siRNA and to form self-assembled nanosystems. In vitro studies performed on HCT 116 cell line, which over express CD44 receptor, demonstrate a receptor-mediated uptake of complexes.

    8. Multi-Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co-Cultured Human Mesenchymal Stem Cells

      Sofia Amadori, Paola Torricelli, Silvia Panzavolta, Annapaola Parrilli, Milena Fini and Adriana Bigi

      Article first published online: 30 JUN 2015 | DOI: 10.1002/mabi.201500165

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      Multi-layered, easily synthesized scaffolds made of gelatin and hydroxyapatite are able to promote chondrogenic and osteogenic differentiation of human mesenchymal stem cells.

    9. Advanced Evaluation Strategies for Protein-Imprinted Polymer Nanobeads

      Bettina Pluhar and Boris Mizaikoff

      Article first published online: 26 JUN 2015 | DOI: 10.1002/mabi.201500106

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      An analytical strategy for providing exhaustive information on the selective binding and inhibition properties of nano-MIPs. Inhibited and active pepsin, respectively, along with two other proteins were incubated to equilibrium with pepsin-imprinted nanobeads, separated from the nanobeads by centrifugation, and the remaining supernatant was analyzed via SDS-PAGE.

  10. Communications

    1. EGFP-Based Protein Nanoparticles with Cell-Penetrating Peptide for Efficient siRNA Delivery

      Xingang Guan, Xiuli Hu, Fengchao Cui, Yunqi Li, Xiabing Jing and Zhigang Xie

      Article first published online: 24 JUN 2015 | DOI: 10.1002/mabi.201500163

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      Fluorescent protein (EGFP) nanoparticles are developed and used for siRNA delivery. Protein nanoparticle can condense siRNA into stable nanoparticle/siRNA complexes, and transfect cy3-labelled siRNA into tumor cells.

  11. Full Papers

    1. Probing the Mucoadhesive Interactions Between Porcine Gastric Mucin and Some Water-Soluble Polymers

      Yasser A. Albarkah, Rebecca J. Green and Vitaliy V. Khutoryanskiy

      Article first published online: 23 JUN 2015 | DOI: 10.1002/mabi.201500158

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      This study reports the structural features of porcine gastric mucin in aqueous dispersions and its interactions with water-soluble polymers. Sonication of mucin dispersion results in disaggregation of particles. However, the interaction of mucin with poly(carboxylic acids) via hydrogen bonding causes further aggregation of its particles.

    2. Hydrolysis-Sensitive Dithiolethione Prodrug Micelles

      Urara Hasegawa, Naoya Tateishi, Hiroshi Uyama and André J. van der Vlies

      Article first published online: 23 JUN 2015 | DOI: 10.1002/mabi.201500156

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      Hydrolysis-sensitive micellar prodrugs of desmethyl anethole dithiolethione (ADTOH), a chemopreventive agent, are prepared from amphiphilic block copolymers composed of a hydrophilic poly(N-acryloyl morpholine) block and a hydrophobic block linking ADT-OH via an ester bond using glycine and isoleucine linkers. This ADT-ester approach enables to modulate the release rate of ADT-OH and thus might find potential application in cancer therapy and prevention.

    3. C-Terminal-oriented Immobilization of Enzymes Using Sortase A-mediated Technique

      Yuto Hata, Takuya Matsumoto, Tsutomu Tanaka and Akihiko Kondo

      Article first published online: 22 JUN 2015 | DOI: 10.1002/mabi.201500113

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      C-Terminal-oriented immobilization of enzymes is demonstrated by sortase A-mediated technique. Although C-terminal-oriented immobilization of enzymes does not provide an improvement of the resistance to pH and thermotolerance, enzymes immobilized by this technique show improved activity and reusability compared to chemically immobilized enzymes. This study indicates that oriented immobilization of enzymes by sortase A may improve the feasibility of industrial utilization of enzymes.

    4. Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment

      Wei Ching Low, Pim-On Rujitanaroj, Dong-Keun Lee, Jinghao Kuang, Phillip B. Messersmith, Jerry Kok Yen Chan and Sing Yian Chew

      Article first published online: 19 JUN 2015 | DOI: 10.1002/mabi.201500101

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      DOPA-melanin (DM) coated nanofibers were functionalized with siRNA to induce REST silencing in mesenchymal stem cells (MSCs) for neuronal differentiation. DM modification enhanced the siRNA loading, release kinetics, and REST knockdown efficiency. Under non-specific differentiation condition, the synergistic effects of REST silencing and nanofiber topography improved MSCs neuronal commitment. Such nanofiber-mediated gene silencing platform may find promising applications in vivo.

    5. Aβ17–20 Peptide-Guided Structuring of Polymeric Conjugates and Their pH-Triggered Dynamic Response

      Sonu Kumar, Varun Bheemireddy and Priyadarsi De

      Article first published online: 17 JUN 2015 | DOI: 10.1002/mabi.201500134

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      “Smart” biohybrid polymers based on amyloidogenic short peptide segment Aβ17-20 are synthesized via RAFT method. The formation of unique nanostructures and α-helix-based secondary structures are obtained governed by peptide moieties. Post-polymerization modification approach is used to prepare PEGylated amphiphilic graft copolymer having imine linkage, which self-assemble into nanoparticles offering pH-triggered drug-release behavior.

    6. Polymer-Coated Magnetic Nanoparticles for Curcumin Delivery to Cancer Cells

      Serena Mancarella, Valentina Greco, Francesca Baldassarre, Daniele Vergara, Michele Maffia and Stefano Leporatti

      Article first published online: 17 JUN 2015 | DOI: 10.1002/mabi.201500142

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      A novel curcumin delivery system based on biodegradable polyelectrolyte-coated iron oxide magnetic nanoparticles is developed. These composite nano-Fe3O4 particles have a suitable size distribution, drug encapsulation efficiency, and drug release kinetics. Curcumin is successfully loaded inside nano-Fe3O4. Cytotoxicity assays show cell viability reduction upon curcumin release demonstrating high efficiency of this novel nanoformulation.

    7. Co-Culture of Human Endothelial Cells and Foreskin Fibroblasts on 3D Silk–Fibrin Scaffolds Supports Vascularization

      Juhi Samal, Stefan Weinandy, Agnieszka Weinandy, Marius Helmedag, Lisanne Rongen, Benita Hermanns-Sachweh, Subhas C. Kundu and Stefan Jockenhoevel

      Article first published online: 12 JUN 2015 | DOI: 10.1002/mabi.201500054

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      Current study hypothesizes that fabricated silk fibroin–fibrin combinatorial scaffolds can support the co-culture of HUVECs and human foreskin fibroblasts to yield an interconnected network of capillary-like structures. Silk fibroin provides the suitable mechanical strength and guides the formation of capillary-like structures in fibrin gel. To our knowledge, it is for the first time that a silk fibroin–fibrin combinatorial scaffold has been developed and used as a matrix to support vascularization.

  12. Communications

    1. PEGylation to Improve Protein Stability During Melt Processing

      Parker Lee, Jenna Towslee, João Maia and Jonathan Pokorski

      Article first published online: 12 JUN 2015 | DOI: 10.1002/mabi.201500143

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      PEG conjugated proteins were melt processed in polymer blends. Protein:PLGA blends were processed at high temperatures using a laboratory-built syringe extruder. The simple covalent conjugation of polyethylene glycol, as is often seen in biopharmaceuticals, provides enhanced dispersion and protein stability in the melt state and post-processing.

  13. Full Papers

    1. Promoting Cell Survival and Proliferation in Degradable Poly(vinyl alcohol)–Tyramine Hydrogels

      Khoon S. Lim, Yogambha Ramaswamy, Justine J. Roberts, Marie-Helene Alves, Laura A. Poole-Warren and Penny J. Martens

      Article first published online: 11 JUN 2015 | DOI: 10.1002/mabi.201500121

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      Antioxidative proteins (sericin and gelatin) are incorporated into PVA–Tyr gels to promote cell survival and proliferation following the photoencapsulation process. Addition of sericin alone is enough to protect cells from the radicals generated during the photocross-linking process, but both sericin and gelatin are needed synergistically to facilitate long-term 3D cell growth and proliferation.

    2. pH-Responsive H-Type PMAA2-b-HTPBN-b-PMAA2 Four-Arm Star Block Copolymer Micelles for PTX Drug Release

      Yan-Ling Luo, Mei Han, Feng Xu, Ya-Shao Chen and Yong-Qin Zhang

      Article first published online: 11 JUN 2015 | DOI: 10.1002/mabi.201500103

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      We are synthesizing H-type PMAA2-b-HTPBN-b-PMAA2 block copolymers. The copolymers could self-assemble into nanoscale spherical core-shell micelle aggregates. The micellization behavior and physicochemical properties are pH-dependent, and the pH phase transition located at 5.7–6.1. The micelle drug formulations exhibited pH-triggered release and cytotoxicity. The H-type block copolymer micelles are promising as drug targeting release carriers.

    3. Soft PEG-Hydrogels with Independently Tunable Stiffness and RGDS-Content for Cell Adhesion Studies

      Anika M. Jonker, Saskia A. Bode, Addie H. Kusters, Jan C. M. van Hest and Dennis W. P. M. Löwik

      Article first published online: 11 JUN 2015 | DOI: 10.1002/mabi.201500110

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      Soft poly(ethylene) glycol (PEG) hydrogels cross-linked via strain-promoted azide-alkyne cycloaddition (SPAAC) are prepared with independently tunable stiffness and RGDS-content. Rheology studies confirms the soft nature of the hydrogels. HOS cells adhere to all RGDS-containing gels independent of gel stiffness. HeLa cells and NIH 3T3 fibroblasts show significantly decreased cell adhesion on the stiffest hydrogel.

    4. Cellular Uptake Behavior of Doxorubicin-Conjugated Nanodiamond Clusters for Efficient Cancer Therapy

      Tae-Kyung Ryu, Gyoung-Ja Lee, Chang-Kyu Rhee and Sung-Wook Choi

      Article first published online: 11 JUN 2015 | DOI: 10.1002/mabi.201500176

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      We prepare doxorubicin (Dox)-conjugated nanodiamonds (ND) clusters with different sizes and systematically demonstrate high cellular uptake and ablation efficiency of Dox-ND clusters for cancer therapy.

    5. Design, Degradation Mechanism and Long-Term Cytotoxicity of Poly(l-lactide) and Poly(Lactide-co-ϵ-Caprolactone) Terpolymer Film and Air-Spun Nanofiber Scaffold

      Gad Sabbatier, Aitor Larrañaga, Andrée-Anne Guay-Bégin, Jorge Fernandez, Florence Diéval, Bernard Durand, Jose-Ramon Sarasua and Gaétan Laroche

      Article first published online: 8 JUN 2015 | DOI: 10.1002/mabi.201500130

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      Innovative degradable terpolymer nanofiber scaffold with finely tuned features lead to a better long-term interaction with specific cells than usual degradable biomaterial. This family of polymer can be used for degradable medical devices and has been applied as vascular biomaterial. Moreover, this article brings substantial new information in general aspects of biodegradable scaffold.

    6. Shell Cross-Linked Polymeric Micelles as Camptothecin Nanocarriers for Anti-HCV Therapy

      Isabel Jiménez-Pardo, Rebeca González-Pastor, Alexandre Lancelot, Rafael Claveria-Gimeno, Adrián Velázquez-Campoy, Olga Abian, M. Blanca Ros and Teresa Sierra

      Article first published online: 5 JUN 2015 | DOI: 10.1002/mabi.201500094

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      Shell photocross-linked polymeric micelles transport efficiently camptothecin into hepatitis C infected cells and show lower toxicity than the free drug. A design based on a hybrid dendritic–linear–dendritic block copolymer yields a macromer that permit effective preparation of stable carriers. An amphiphilic fluorescent probe proves the internalization of the carriers within the cells.

    7. Biodegradable Nanotopography Combined with Neurotrophic Signals Enhances Contact Guidance and Neuronal Differentiation of Human Neural Stem Cells

      Kisuk Yang, Esther Park, Jong Seung Lee, Il-Sun Kim, Kwonho Hong, Kook In Park, Seung-Woo Cho and Hee Seok Yang

      Article first published online: 2 JUN 2015 | DOI: 10.1002/mabi.201500080

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      Biodegradable PLGA-patterned substrates with immobilization of ECM proteins via a DOPA coating significantly promote contact guidance and neuronal differentiation of hNSCs. The addition of neurotrophic factor (NGF) further enhances neurogenesis of hNSCs, indicating a synergistic effect of biophysical and biochemical cues on hNSC differentiation.

  14. Communications

    1. Instructive Conductive 3D Silk Foam-Based Bone Tissue Scaffolds Enable Electrical Stimulation of Stem Cells for Enhanced Osteogenic Differentiation

      John G. Hardy, Sydney A. Geissler, David Aguilar Jr., Maria K. Villancio-Wolter, David J. Mouser, Rushi C. Sukhavasi, R. Chase Cornelison, Lee W. Tien, R. Carmen Preda, Rebecca S. Hayden, Jacqueline K. Chow, Lindsey Nguy, David L. Kaplan and Christine E. Schmidt

      Article first published online: 1 JUN 2015 | DOI: 10.1002/mabi.201500171

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      Instructive tissue scaffolds capable of controlling the behavior of the cells that reside within them are particularly interesting for tissue engineering. Herein we describe the preparation of conductive silk foam-based bone tissue scaffolds that enable the electrical stimulation of human mesenchymal stem cells to enhance their differentiation toward osteogenic outcomes.

  15. Full Papers

    1. Hyaluronic Acid/Poly-l-Lysine Multilayers as Reservoirs for Storage and Release of Small Charged Molecules

      Vladimir Z. Prokopović, Claus Duschl and Dmitry Volodkin

      Article first published online: 15 MAY 2015 | DOI: 10.1002/mabi.201500093

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      Polyelectrolyte multilayer films composed of biocompatible polymers hyaluronic acid and poly-l-lysine are assembled and their ability to store and release small charged molecules is studied. The films show high storage capacity and sustain release profiles, and these phenomena are explained by the proposed mechanism based on charge balance and polymer dynamics in the film.

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