© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Edited By: Alois Jungbauer and Sang Yup Lee
Impact Factor: 3.781
ISI Journal Citation Reports © Ranking: 2015: 17/77 (BIOCHEMICAL RESEARCH METHODS); 34/161 (Biotechnology & Applied Microbiology)
Online ISSN: 1860-7314
Recently Published Issues
Congratulations to the winning cover of BTJ's 2016 cover contest!
The October issue cover was provided by the authors of the article Chlorella species as hosts for genetic engineering and expression of heterologous proteins: Progress, challenge and perspective, Bo Yang, Jin Liu, Yue Jiang and Feng Chen. The winner of the book voucher was informed via email. Many thanks to the more than 1000 participants who were voting for these covers!
Recently Published Articles
- You have full text access to this OnlineOpen articleAn automated laboratory-scale methodology for the generation of sheared mammalian cell culture samples
Adrian Joseph, Stephen Goldrick, Michael Mollet, Richard Turner, Jean Bender, David Gruber, Suzanne S. Farid and Nigel Titchener-Hooker
Version of Record online: 22 MAR 2017 | DOI: 10.1002/biot.201600730
Disk-stack centrifuges are commonly used for the removal of cells and cell debris when harvesting large volumes of mammalian cell culture broth. However, the characterization of the levels of shear in the feed-zone region of a disk-stack centrifuge is often difficult to quantify. This work details a cost-effective and rapid method that can be used to define the levels of shear present in disk-stack centrifuges across a range of platforms.
- High throughput inclusion body sizing: Nano particle tracking analysis
Wieland N. Reichelt, Andreas Kaineder, Markus Brillmann, Lukas Neutsch, Alexander Taschauer, Hans Lohninger and Christoph Herwig
Accepted manuscript online: 16 MAR 2017 08:51AM EST | DOI: 10.1002/biot.201600471
- You have full text access to this OnlineOpen articleAnimal component-free Agrobacterium tumefaciens cultivation media for better GMP-compliance increases biomass yield and pharmaceutical protein expression in Nicotiana benthamiana
Marcel Houdelet, Anna Galinski, Tanja Holland, Kathrin Wenzel, Stefan Schillberg and Johannes Felix Buyel
Version of Record online: 16 MAR 2017 | DOI: 10.1002/biot.201600721
Transient protein expression in plants is a rapid method to produce recombinant proteins, e.g. for pandemic emergency cases. All animal-derived components in A. tumefaciens cultivation media were replaced by plant-derived counterparts increasing GMP (good manufacturing practice)-compatibility. A DoE (design of experiments)-based approach was used to optimize the new medium in terms of bacterial growth and product accumulation during transient expression in plants, improving economic process viability.
- Dual display of proteins on the yeast cell surface simplifies quantification of binding interactions and enzymatic bioconjugation reactions
Sungwon Lim, Jeff E. Glasgow, Maria Filsinger Interrante, Erica M. Storm and Jennifer R. Cochran
Accepted manuscript online: 16 MAR 2017 03:30AM EST | DOI: 10.1002/biot.201600696
- A Cleavable Self-Assembling Tag Strategy for Preparing Proteins and Peptides with an Authentic N-Terminus
Qing Zhao, Bihong Zhou, Xianxing Gao, Lei Xing, Xu Wang and Zhanglin Lin
Accepted manuscript online: 15 MAR 2017 10:21AM EST | DOI: 10.1002/biot.201600656