© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Edited By: Alois Jungbauer and Sang Yup Lee
Impact Factor: 3.781
ISI Journal Citation Reports © Ranking: 2015: 17/77 (BIOCHEMICAL RESEARCH METHODS); 34/161 (Biotechnology & Applied Microbiology)
Online ISSN: 1860-7314
Recently Published Issues
Congratulations to the winning cover of BTJ's 2016 cover contest!
The October issue cover was provided by the authors of the article Chlorella species as hosts for genetic engineering and expression of heterologous proteins: Progress, challenge and perspective, Bo Yang, Jin Liu, Yue Jiang and Feng Chen. The winner of the book voucher was informed via email. Many thanks to the more than 1000 participants who were voting for these covers!
Recently Published Articles
- Production of taxadiene by engineering of mevalonate pathway in Escherichia coli and endophytic fungus Alternaria alternata TPF6
Guangkai Bian, Yujie Yuan, Hui Tao, Xiaofei Shi, Xiaofang Zhong, Yichao Han, Shuai Fu, Chengxiang Fang, Zixin Deng and Tiangang Liu
Accepted manuscript online: 20 FEB 2017 03:00AM EST | DOI: 10.1002/biot.201600697
- Physiological capacities decline during induced bioprocesses leading to substrate accumulation
Wieland N. Reichelt, Markus Brillmann, Peter Thurrold, Peter Keil, Jens Fricke and Christoph Herwig
Version of Record online: 17 FEB 2017 | DOI: 10.1002/biot.201600547
- Multi-objective global optimization (MOGO): Algorithm and case study in gradient elution chromatography
Lars Freier and Eric von Lieres
Version of Record online: 16 FEB 2017 | DOI: 10.1002/biot.201600613
Gaussian Process Regression (GPR) is used for interpolating given measurement data with sound confidence intervals. This information is applied for designing further experiments such as to maximize the expected improvement over the current best objective value. An iterative procedure enables global optimization for single and multiple objectives.
- Bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation in E. coli
Mingzhi Liang, Stefanie Frank, Heinrich Lünsdorf, Martin J. Warren and Michael B. Prentice
Version of Record online: 10 FEB 2017 | DOI: 10.1002/biot.201600415
Polyphosphate is a polymer which provides phosphate and energy reserves to microorganisms. Levels of polyphosphate in bacteria change in response to the environment and growth phase with accompanying excretion or uptake of phosphate. Environmental manipulation of wastewater bacteria to make them increase phosphate stores causes them to take up phosphate, which is useful as part of water treatment, but they later release the phosphate back into the wastewater. In this study the authors show that targeting the polymerising enzyme polyphosphate kinase (PPK1) to bacterial microcompartments in E. coli causes stable polyphosphate storage by sequestering it away from other depolymerising enzymes inside the bacteria. These bacteria now take up phosphate without subsequent excretion.
- Tenocytic extract and mechanical stimulation in a tissue-engineered tendon construct increases cellular proliferation and ECM deposition
Brandon Engebretson, Zachary R. Mussett and Vassilios I. Sikavitsas
Version of Record online: 10 FEB 2017 | DOI: 10.1002/biot.201600595
Growth factors are regularly added to stem cell cultures to encourage differentiation towards a desired lineage. In this study, the authors demonstrate that a cell free extract, obtained by the lysing of mature tenocytes, can be used in lieu of other more expensive commercial growth factors in tendon tissue engineering cultures. This tenocyte extract, when combined with mechanostimulation, is shown to increase cellularity and tensile strength of our tendon constructs, yielding a more tendon like phenotype for transplantation.