Biotechnology Journal

Cover image for Vol. 12 Issue 9

Edited By: Alois Jungbauer and Sang Yup Lee

Impact Factor: 3.649

ISI Journal Citation Reports © Ranking: 2016: 23/77 (BIOCHEMICAL RESEARCH METHODS); 39/158 (Biotechnology & Applied Microbiology)

Online ISSN: 1860-7314

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Congratulations to the winning cover of BTJ's 2016 cover contest!

Biotechnology Journal 2016 cover contest winner: October cover

The October issue cover was provided by the authors of the article Chlorella species as hosts for genetic engineering and expression of heterologous proteins: Progress, challenge and perspective, Bo Yang, Jin Liu, Yue Jiang and Feng Chen. The winner of the book voucher was informed via email. Many thanks to the more than 1000 participants who were voting for these covers!


CRISPR autumn collection

Recently Published Articles

  1. You have full text access to this OnlineOpen article
    Covalent Binding of Bone Morphogenetic Protein-2 and Transforming Growth Factor-β3 to 3D Plotted Scaffolds for Osteochondral Tissue Regeneration

    Andrea Di Luca, Michel Klein-Gunnewiek, Julius G. Vancso, Clemens A. van Blitterswijk, Edmondo M. Benetti and Lorenzo Moroni

    Version of Record online: 25 SEP 2017 | DOI: 10.1002/biot.201700072

    Thumbnail image of graphical abstract

    3D scaffolds coupled to biological factors such as growth factors could have the potential to steer cell activity. Homogeneous covalent binding of growth factors showed enhanced gene expression for cartilage and bone tissue regeneration, associated to increased protein synthesis for bone regeneration. The possibility to create covalently bound gradients of growth factors is also explored. This article is part of an AFOB (Asian Federation of Biotechnology) Special issue. To learn more about the AFOB, visit

  2. Expansion of Bone Marrow Mesenchymal Stromal Cells in Perfused 3D Ceramic Scaffolds Enhances In Vivo Bone Formation

    Allison I. Hoch, Ralph Duhr, Nunzia Di Maggio, Arne Mehrkens, Marcel Jakob and David Wendt

    Version of Record online: 25 SEP 2017 | DOI: 10.1002/biot.201700071

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    Bone marrow-derived mesenchymal stromal cells (BMSC), when expanded directly within 3D ceramic scaffolds in perfusion bioreactors, more reproducibly form bone when implanted in vivo as compared to conventional expansion on 2D polystyrene dishes/flasks. Since the bioreactor-based expansion on 3D ceramic scaffolds encompasses multiple aspects that are inherently different from expansion on 2D polystyrene, the authors aimed to decouple the effects of specific parameters among these two model systems. This article is part of an AFOB (Asian Federation of Biotechnology) Special issue. To learn more about the AFOB visit

  3. Electrospun Nanofibrous Meshes Cultured With Wharton's Jelly Stem Cell: An Alternative for Cartilage Regeneration, Without the Need of Growth Factors

    Marta Alves da Silva, Albino Martins, Ana R. Costa-Pinto, Nélson Monteiro, Susana Faria, Rui L. Reis and Nuno M. Neves

    Version of Record online: 25 SEP 2017 | DOI: 10.1002/biot.201700073

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    Human Wharton's Jelly stem cells where isolated and cultured without external chondrogenic stimulation onto polycaprolactone nanofiber meshes. At the end of the experiment, cartilage-like tissue is obtained. This tissue shows significant differences compared to the control conditions (using human articular chondrocytes): improved ECM characteristics and gene expression. This article is part of an AFOB (Asian Federation of Biotechnology) Special issue. To learn more about the AFOB, visit

  4. Engineering Microbial Metabolite Dynamics and Heterogeneity

    Alexander C. Schmitz, Christopher J. Hartline and Fuzhong Zhang

    Version of Record online: 25 SEP 2017 | DOI: 10.1002/biot.201700422

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    Metabolite dynamics and metabolite heterogeneity have a profound impact on the bioproduction of chemical compounds. The authors present the current understanding of factors that contribute to metabolite dyanmics and heterogeneity and how these factors have informed metabolic engineering strategies for increased chemical production in bio-processes.

  5. Optimized selection marker and CHO host cell combinations for generating high monoclonal antibody producing cell lines

    Jessna H.M. Yeo, Steven C.L. Ho, Mariati  , Esther Koh, Shi Jie Tay, Susanto Woen, Peiqing Zhang and Yuansheng Yang

    Accepted manuscript online: 25 SEP 2017 09:12AM EST | DOI: 10.1002/biot.201700175