© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Edited By: Alois Jungbauer and Sang Yup Lee
Impact Factor: 3.781
ISI Journal Citation Reports © Ranking: 2015: 17/77 (BIOCHEMICAL RESEARCH METHODS); 34/161 (Biotechnology & Applied Microbiology)
Online ISSN: 1860-7314
Recently Published Issues
Congratulations to the winning cover of BTJ's 2016 cover contest!
The October issue cover was provided by the authors of the article Chlorella species as hosts for genetic engineering and expression of heterologous proteins: Progress, challenge and perspective, Bo Yang, Jin Liu, Yue Jiang and Feng Chen. The winner of the book voucher was informed via email. Many thanks to the more than 1000 participants who were voting for these covers!
Recently Published Articles
- You have full text access to this OnlineOpen articleStreptomyces venezuelae TX-TL – a next generation cell-free synthetic biology tool
Simon J. Moore, Hung-En Lai, Hannah Needham, Karen M. Polizzi and Paul S. Freemont
Version of Record online: 24 FEB 2017 | DOI: 10.1002/biot.201600678
Streptomyces venezuelae is a developing host for synthetic biology and metabolic engineering. To accelerate the understanding of this host and access secondary metabolism enzymes, the authors have characterized a high-yield S. venezuelae in vitro transcription-translation (TX-TL) system for synthetic biology. The system utilizes 3-phosphoglyceric acid (3-PGA) to regenerate energy and has been shown to synthesize up to 1.3 µM of sfGFP (under batch synthesis) and a range of enzymes from the oxytetracycline biosynthesis pathway.
- You have full text access to this OnlineOpen articleReal-time pH monitoring of industrially relevant enzymatic reactions in a microfluidic side-entry reactor (μSER) shows potential for pH control
Pia Gruber, Marco P.C. Marques, Philipp Sulzer, Roland Wohlgemuth, Torsten Mayr, Frank Baganz and Nicolas Szita
Version of Record online: 24 FEB 2017 | DOI: 10.1002/biot.201600475
The pH is an important parameter for the efficiency of enzymatic reactions. Small volume flow reactors, also known as microfluidic reactors, can be used to optimise enzymatic reactions at low cost. Therefore, being able to measure the pH in a microfluidic reactor is very important. In this contribution the authors demonstrate that the measurement of pH in such a reactor is possible by integrating pH sensors at multiple locations. These sensors were used to measure the pH in two enzymatic reactions and it was shown that the pH could be adjusted to remain within boundaries ideal for the enzymes.
- An Automated Laboratory-scale Methodology for the Generation of Sheared Mammalian Cell Culture Samples
Adrian Joseph, Stephen Goldrick, Michael Mollet, Richard Turner, Jean Bender, David Gruber, Suzanne S Farid and Nigel Titchener-Hooker
Accepted manuscript online: 24 FEB 2017 02:15AM EST | DOI: 10.1002/biot.201600730
- Disruption of genes involved in CORVET complex leads to enhanced secretion of heterologous carboxylesterase only in protease deficient Pichia pastoris
Lukas Marsalek, Clemens Gruber, Friedrich Altmann, Markus Aleschko, Diethard Mattanovich, Brigitte Gasser and Verena Puxbaum
Accepted manuscript online: 23 FEB 2017 05:35AM EST | DOI: 10.1002/biot.201600584
- Animal component-free Agrobacterium tumefaciens cultivation media for better GMP-compliance increases biomass yield and pharmaceutical protein expression in Nicotiana benthamiana
Marcel Houdelet, Anna Galinski, Tanja Holland, Kathrin Wenzel, Stefan Schillberg and Johannes Felix Buyel
Accepted manuscript online: 21 FEB 2017 10:30AM EST | DOI: 10.1002/biot.201600721