Biotechnology Journal

Cover image for Vol. 13 Issue 2

Editor: Alois Jungbauer and Sang Yup Lee

Impact Factor: 3.649

ISI Journal Citation Reports © Ranking: 2016: 23/78 (Biochemical Research Methods); 41/160 (Biotechnology & Applied Microbiology)

Online ISSN: 1860-7314

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  • Current Issue:February 2018

    Volume 13, Issue 2

  • January 2018

    Volume 13, Issue 1

    Special Issue: Biotech Methods and Advances

  • December 2017

    Volume 12, Issue 12

    Special Issue: AFOB Special Issue on Stem Cells in...

  • November 2017

    Volume 12, Issue 11

    Special Issue: AFOB Special Issue on Industrial Bi...

  • October 2017

    Volume 12, Issue 10

    Special Issue: Focus Issue: Metabolic Science: Dri...

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Congratulations to the winners of our cover competition! The first prize goes to the Inside Cover Picture of the October Special issue on "Metabolic Science", and was prepared by Alessandro Pellis, Marco Vastano, Felice Quartinello, Enrique Herrero Acero and Georg M. Guebitz.

Metabolic Engineering 12

June 24-28, 2018, The Westin Grand Munich, Munich, Germany

Recently Published Articles

  1. Enzymatic production of biodiesel: strategies to overcome methanol inactivation

    Marina Lotti, Jürgen Pleiss, Francisco Valero and Pau Ferrer

    Accepted manuscript online: 20 FEB 2018 09:00AM EST | DOI: 10.1002/biot.201700155

  2. Heat Shock Protein Reports on Proteome Stress

    Yu Liu and Xin Zhang

    Accepted manuscript online: 20 FEB 2018 09:00AM EST | DOI: 10.1002/biot.201800039

  3. Impact of CHO Metabolism on Cell Growth and Protein Production: An Overview of Toxic and Inhibiting Metabolites and Nutrients

    Sara Pereira, Helene Faustrup Kildegaard and Mikael Rørdam Andersen

    Version of Record online: 19 FEB 2018 | DOI: 10.1002/biot.201700499

    Thumbnail image of graphical abstract

    Chinese hamster ovary (CHO) cells are the chosen expression platform for the production of therapeutic proteins. ‘Omics data sets can aid and guide the rational design and generation of mammalian cells factories free of unwanted metabolic products and therefore capable of achieving higher cell densities and productivities. In this review, a number of metabolites depleting and accumulating throughout the course of cultivations, that have toxic and growth inhibitory effects to the cells are presented. Additionally, the potential application of this knowledge along with the use of analytical methods and on-line metabolic resources for enhancing CHO cell lines by combining media development and cell line engineering approaches is discussed.

  4. Virus-like particle engineering: From rational design to versatile applications

    Xuanwei Ding, Dong Liu, George Booth, Wei Gao and Yuan Lu

    Accepted manuscript online: 17 FEB 2018 06:20AM EST | DOI: 10.1002/biot.201700324

  5. You have full text access to this OnlineOpen article
    OPP Labeling Enables Total Protein Synthesis Quantification in CHO Production Cell Lines at the Single-Cell Level

    Fabian Nagelreiter, Michael T. Coats, Gerald Klanert, Elisabeth Gludovacz, Nicole Borth, Johannes Grillari and Markus Schosserer

    Version of Record online: 15 FEB 2018 | DOI: 10.1002/biot.201700492

    Thumbnail image of graphical abstract

    O-propargyl-puromycin (OPP) is incorporated into the nascent polypeptide chain during translation. A fluorophore is then covalently attached to OPP, which allows measurement of global protein synthesis via flow cytometry. We provide proof-of-principle that total protein synthesis rates can be analyzed on a single cell level in combination with other cellular parameters such as recombinant protein production and DNA content in Chinese hamster ovary cells.