Biotechnology Journal

Cover image for Vol. 12 Issue 1

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Alois Jungbauer and Sang Yup Lee

Impact Factor: 3.781

ISI Journal Citation Reports © Ranking: 2015: 17/77 (BIOCHEMICAL RESEARCH METHODS); 34/161 (Biotechnology & Applied Microbiology)

Online ISSN: 1860-7314


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  1. Reviews

    1. Bringing 3D tumor models to the clinic – predictive value for personalized medicine

      Kathrin Halfter and Dr. Barbara Mayer

      Version of Record online: 18 JAN 2017 | DOI: 10.1002/biot.201600295

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      This review discusses the use of three-dimensional (3D) cell culture models as a preclinical testing system for clinical cancer treatment. A literature search revealed only a small number of conducted studies. This may explain the current reluctance in the use of this technology in the clinical routine despite potential benefit for patients.

  2. Research Articles

    1. Smartphone-based portable wireless optical system for the detection of target analytes

      Shreedhar Gautam, Bhagwan S Batule, Hyo Yong Kim, Ki Soo Park and Hyun Gyu Park

      Version of Record online: 18 JAN 2017 | DOI: 10.1002/biot.201600581

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      A novel smartphone-based Portable and Wireless Optical System (PAWS) for rapid, quantitative, and on-site analysis of target analytes is descirbed. Colorimetric signal is generated in presence of the target molecules which is converted to an electrical signal by the inbuilt electronic circuit of the device. The converted electrical signal is then measured wirelessly via multimeter in the smartphone which processes the data and displays the results, including the concentration of analytes and its significance. This handheld device shows great potential as a programmable and miniaturized platform to achieve rapid and on-site detection of various analytes in a point-of-care testing (POCT) manner.

    2. Genome analysis of a hyper acetone-butanol-ethanol (ABE) producing Clostridium acetobutylicum BKM19

      Changhee Cho, Donghui Choe, Yu-Sin Jang, Kyung-Jin Kim, Won Jun Kim, Byung-Kwan Cho, E. Terry Papoutsakis, George N. Bennett, Do Young Seung and Sang Yup Lee

      Version of Record online: 16 JAN 2017 | DOI: 10.1002/biot.201600457

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      A hyper acetone-butanol-ethanol (ABE) producing Clostridium acetobutylicum BKM19 strain and its parental strain PJC4BK (the buk mutant) are sequenced to identify the mutations responsible for enhanced solvents production. In comparison with the C. acetobutylicum PJC4BK genome, 13 single nucleotide variants (SNVs), 1 deletion, and 1 back mutation are found in the C. acetobutylicum BKM19 genome.

    3. Metabolic engineering of Mannheimia succiniciproducens for succinic acid production based on elementary mode analysis with clustering

      Won Jun Kim, Jung Ho Ahn, Hyun Uk Kim, Tae Yong Kim and Sang Yup Lee

      Version of Record online: 16 JAN 2017 | DOI: 10.1002/biot.201600701

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      Elementary mode analysis with clustering (EMC) was used to analyze the metabolic network of Mannheimia succiniciproducens for succinic acid overproduction. Systems metabolic engineering with the EMC analysis identified novel gene overexpression targets that enhanced the succinic acid production. The EMC analysis presented in this study can also be deployed in metabolic engineering of other microorganisms to overproduce succinic acid and other chemicals of interest in an intuitive manner.

  3. Biotech Methods

    1. Predictive glycoengineering of biosimilars using a Markov chain glycosylation model

      Philipp N. Spahn, Anders H. Hansen, Stefan Kol, Bjørn G. Voldborg and Nathan E. Lewis

      Version of Record online: 28 DEC 2016 | DOI: 10.1002/biot.201600489

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      Biosimilars must resemble the glycoprofile of the original protein drug. The authors present a computational method to predict the required perturbations in the glycosylation reaction network that will match a glycoprofile from a production cell line with the glycoprofile of the original product.

  4. Research Articles

    1. High-throughput downstream process development for cell-based products using aqueous two-phase systems (ATPS) – A case study

      Sarah Zimmermann, Christian Scheeder, Philipp K Zimmermann, Are Bogsnes, Mattias Hansson, Arne Staby and Jürgen Hubbuch

      Version of Record online: 21 DEC 2016 | DOI: 10.1002/biot.201600587

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      Aqueous two-phase systems (ATPS) offer a gentle and cost-effective means to purify therapeutically relevant cell types based on their surface properties. In this case study a high-throughput screening (HTS) platform was used to design a purification strategy for two model cell lines. The study shows how HTS in combination with multi-stage models are an effective tool to design purification processes and that the applied models are highly accurate. Finally, functional analysis showed how cell cycle distribution affects cell partitioning in ATPS.

  5. Rapid Communications

    1. Aerobic expression of Vitreoscilla hemoglobin improves the growth performance of CHO-K1 cells

      Mariana Juárez, Claudia H. González-De la Rosa, Elisa Memún, Juan-Carlos Sigala and Alvaro R. Lara

      Version of Record online: 21 DEC 2016 | DOI: 10.1002/biot.201600438

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      The hemoglobin from Vitreoscilla stercoraria (VHb) has been widely applied for improving the microaerobic metabolism of microbial cells. However, much less is known about the effect of VHb on mammalian cell metabolism. It has been proposed that VHb plays a role in delivering oxygen to the electron transport chain, which has been useful to minimize the aerobic acetate production by Escherichia coli. In this work a codon optimized VHb gene was expressed in CHO cells culture. The presence of VHb improved cell viability and growth rate, while strongly decreased the lactate yield per cell. This is useful for the design of CHO cells with better performance under process conditions.

  6. Biotech Methods

    1. A simplified procedure for antibody engineering by yeast surface display: Coupling display levels and target binding by ribosomal skipping

      Julius Grzeschik, Steffen C. Hinz, Doreen Könning, Thomas Pirzer, Stefan Becker, Stefan Zielonka and Harald Kolmar

      Version of Record online: 20 DEC 2016 | DOI: 10.1002/biot.201600454

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      Yeast surface display is a widely used method for protein engineering. However, current yeast display applications rely on the staining of epitope tags in order to verify full-length presentation of the protein of interest. A novel method for yeast display that relies on ribosomal skipping and omits the need for immunofluorescence stainings of epitope tags, thereby being more cost- and time-efficient is described.

  7. Rapid Communications

    1. Simultaneous conversion of free fatty acids and triglycerides to biodiesel by immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase

      Jerome Amoah, Emmanuel Quayson, Shinji Hama, Ayumi Yoshida, Tomohisa Hasunuma, Chiaki Ogino and Akihiko Kondo

      Version of Record online: 15 DEC 2016 | DOI: 10.1002/biot.201600400

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      The high free fatty acids (FFA) in oil has been a hurdle in one-step biodiesel production. A lipase with both transesterification and esterification activity and has high water tolerance was successfully used for the conversion. This technique can be used for industrial production of biodiesel from waste cooking oils and microbial oils without pre-treatment.

  8. Research Articles

    1. You have full text access to this OnlineOpen article
      Immobilized hematopoietic growth factors onto magnetic particles offer a scalable strategy for cell therapy manufacturing in suspension cultures

      Matthew J. Worrallo, Rebecca L.L. Moore, Katie E. Glen and Robert J. Thomas

      Version of Record online: 1 DEC 2016 | DOI: 10.1002/biot.201600493

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      Therapeutic products based on blood system cells, in particular various immune cells, are entering the clinical market or in development with dramatic clinical benefits for a range of life threatening conditions such as cancer. These cells need new manufacturing technology to ensure cost and quality can be controlled to the level required for medicinal products. This work describes a new way to provide important factors for the growth of these cells outside the body that will help optimise and control product manufacture.

  9. Reviews

    1. Nanomaterials-based biosensors for detection of microorganisms and microbial toxins

      Laura Sutarlie, Sian Yang Ow and Xiaodi Su

      Version of Record online: 27 OCT 2016 | DOI: 10.1002/biot.201500459

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      The detection of microorganisms and their toxins in food and the environment are important for health and safety reasons. This review summarizes the various nanoparticle-based methods to detect microorganisms and their toxins from environmental samples as well as the implications and possibilities for adaptation of these methods into an on-site rapid detection system. This article is part of an AFOB (Asian Federation of Biotechnology) Special issue. To learn more about the AFOB, visit

  10. Research Articles

    1. You have full text access to this OnlineOpen article
      Improved production of propionic acid using genome shuffling

      Carlos H Luna-Flores, Robin W Palfreyman, Jens O Krömer, Lars K Nielsen and Esteban Marcellin

      Version of Record online: 20 OCT 2016 | DOI: 10.1002/biot.201600120

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      Traditionally derived from oil-based resources, biological production of propionic acid (PA) using Propionibacterium has gained interest in recent years. In this study, genome shuffling on two P. acidipropionici strains was used to increase growth rate and PA production. Sequencing of the new strains suggests that recombination events, in well-conserved regions of the genome, are responsible for the phenotype improvement. This work provides base resolution insight on the underlying mechanisms leading to a higher PA production using genome shuffling.

    2. You have full text access to this OnlineOpen article
      Camelid VHH affinity ligands enable separation of closely related biopharmaceuticals

      Timothy M. Pabst, Michaela Wendeler, Xiangyang Wang, Sandra Bezemer, Pim Hermans and Alan K. Hunter

      Version of Record online: 20 OCT 2016 | DOI: 10.1002/biot.201600357

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      Separation of closely related, but less biologically active, forms of biopharmaceuticals often requires that the purification process be operated under conditions that are low-yielding and/or manufacturing unfriendly. In this study, the authors demonstrate the use of highly selective camelid VHH affinity chromatography for the separation of active proteins from inactive protein variants that differ by as little as one amino acid or a minor change in a post-translational modification.

  11. Rapid Communications

    1. Polyester hydrolysis is enhanced by a truncated esterase: Less is more

      Antonino Biundo, Doris Ribitsch, Georg Steinkellner, Karl Gruber and Georg M. Guebitz

      Version of Record online: 11 OCT 2016 | DOI: 10.1002/biot.201600450

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      Enzymes are able to hydrolyze the surface of polymer substrates. In this study, the authors prove that the truncation of the N-terminus of the esterase Cbotu_EstA improves its adsorption on polyethylene terephthalate (PET) surfaces and the hydrolysis of ester bonds releasing products which can be detected to analyze the enzymatic activity.

  12. Research Articles

    1. You have full text access to this OnlineOpen article
      A versatile modular bioreactor platform for Tissue Engineering

      Sebastian Schuerlein, Thomas Schwarz, Steffan Krziminski, Sabine Gätzner, Anke Hoppensack, Ivo Schwedhelm, Matthias Schweinlin, Heike Walles and Jan Hansmann

      Version of Record online: 26 SEP 2016 | DOI: 10.1002/biot.201600326

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      Generating tissues or organs by using Tissue Engineering requires complex culture conditions beyond the functionality of standard cell culture incubators. In this study an automated, compact, flexible, modular bioreactor platform addressing the requirements of Tissue Engineering applications is developed. Therefore, the modules were combined according to specific Tissue Engineering processes.

  13. Biotech Methods

    1. You have free access to this content
      CellShape: A user-friendly image analysis tool for quantitative visualization of bacterial cell factories inside

      Ángel Goñi-Moreno, Juhyun Kim and Víctor de Lorenzo

      Version of Record online: 7 SEP 2016 | DOI: 10.1002/biot.201600323

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      The CellShape tool allows translation of fluo-rescence microcopy observations of single bacteria into a quantitative description of the inside constituents of the cell


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