Journal of Biophotonics

Cover image for Vol. 9 Issue 10

Early View (Online Version of Record published before inclusion in an issue)

Editor: Jürgen Popp

Impact Factor: 3.818

ISI Journal Citation Reports © Ranking: 2015: 10/90 (Optics); 16/77 (BIOCHEMICAL RESEARCH METHODS); 16/72 (Biophysics)

Online ISSN: 1864-0648

Associated Title(s): Laser & Photonics Reviews

  1. Full Articles

    1. Reference-independent wide field fluorescence lifetime measurements using Frequency-Domain (FD) technique based on phase and amplitude crossing point

      Gilad Yahav, Eran Barnoy, Nir Roth, Lior Turgeman and Dror Fixler

      Version of Record online: 24 OCT 2016 | DOI: 10.1002/jbio.201600220

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      Fluorescence lifetime imaging microscopy (FLIM) is an essential tool in many scientific fields such as biology and medicine thanks to the known advantages of the fluorescence lifetime (FLT) over the classical fluorescence intensity (FI). However, the frequency domain (FD) FLIM technique suffers from its strong dependence on the reference and its compliance to the sample. In this paper, we suggest a new way to calculate the FLT by using the crossing point (CRPO) between the modulation and phase FLTs measured over several light emitting diode (LED) DC currents values.

      This new technique was validated by measuring homogeneous substances with known FLT, where the CRPO appears to be the optimal measuring point. Furthermore, the CRPO method was applied in heterogeneous samples. It was found that the CRPO applied to known mixed solutions provides the weighted average of the FLT of the used solutions. While measuring B16 and lymphocyte cells, the CRPO FLT of the DAPI compound in single FLT regions was measured at 3.5 ± 0.06ns and at 2.83 ± 0.07 ns, respectively, both of which match previous reports and multi-frequency analyses. This paper suggests the CRPO as a new method to extract the FLT in problematic cases such as high MCP gains and heterogeneous environments.

    2. Improved quantification of collagen anisotropy with polarization-resolved second harmonic generation microscopy

      Radu Hristu, Stefan G. Stanciu, Denis E. Tranca and George A. Stanciu

      Version of Record online: 24 OCT 2016 | DOI: 10.1002/jbio.201600197

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      We propose a method for choosing the optimal laser beam polarization in SHG imaging for calculating the anisotropy factor used in assessing collagen anisotropy in healthy against pathological tissue. We demonstrate the method for the differentiation between healthy and dysplastic areas on skin tissue slices, but argue it can be equally applied to enhance the characterization of other tissue categories, either H&E stained or non-stained, fixed or freshly excised.

    3. Quantitative phase-filtered wavelength-modulated differential photoacoustic radar tumor hypoxia imaging toward early cancer detection

      Edem Dovlo, Bahman Lashkari, Sung soo Sean Choi, Andreas Mandelis, Wei Shi and Fei-Fei Liu

      Version of Record online: 19 OCT 2016 | DOI: 10.1002/jbio.201600168

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      Wavelength-modulated differential photoacoustic radar (WM-DPAR) imaging utilizes chirp modulated laser beams at two distinct wavelengths for non-invasive early cancer detection via the sensitive characterization of functional information such as hemoglobin oxygenation (SO2) levels and total hemoglobin concentration (tHb). Due to the out-of-phase modulation of the lasers, background absorption is highly suppressed while the difference between the two photoacoustic signals is amplified. Minute changes in tHb and SO2 are thereby detectable, allowing for pre-malignant tumor identification (before becoming anatomically apparent) and hypoxia monitoring.

    4. Intravital excitation increases detection sensitivity for pulmonary tuberculosis by whole-body imaging with β-lactamase reporter enzyme fluorescence

      Fatemeh Nooshabadi, Hee-Jeong Yang, Yunfeng Cheng, Madeleine S. Durkee, Hexin Xie, Jianghong Rao, Jeffrey D. Cirillo and Kristen C. Maitland

      Version of Record online: 18 OCT 2016 | DOI: 10.1002/jbio.201600132

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      We demonstrate an integrated imaging platform that increases the sensitivity of in vivo optical detection of Mycobacterium tuberculosis (Mtb) in mouse lungs. Mtb-specific near-infrared fluorogenic substrate is detected with whole-animal imaging and fiber-microendoscope intravital illumination. By combining these bacteria-sensing methodologies, detection threshold was improved by 100× over whole-animal imaging with epi-illumination, and by 10× over previous intravital illumination experiments with recombinant reporter strains in the visible range.

  2. Letters

    1. Photoacoustic microscopy of electronic acupuncture (EA) effect in small animals

      Jinge Yang, Dan Wu, Yong Tang and Huabei Jiang

      Version of Record online: 18 OCT 2016 | DOI: 10.1002/jbio.201600210

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      Acoustic-resolution photoacoustic microscopy (AR-PAM) system were used could to probe changes in hemoglobin concentration of cortical regions caused by electroacupuncture. Remarkable CBV changes in sensorimotor and retrosplenial agranular cortex were observed. Results showed the potential of PAM as a visualization tool to study the acupuncture effect on brain hemodynamics in animal models.

  3. Full Articles

    1. Distinction between breast cancer cell subtypes using third harmonic generation microscopy

      Evangelia Gavgiotaki, George Filippidis, Haris Markomanolaki, George Kenanakis, Sofia Agelaki, Vassilis Georgoulias and Irene Athanassakis

      Version of Record online: 18 OCT 2016 | DOI: 10.1002/jbio.201600173

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      Third Harmonic Generation (THG) microscopy as a non-invasive, label free imaging methodology, allows linkage of lipid profiles with various breast cancer cells. The collected THG signal arise mostly from the lipid droplets and the membrane lipid bilayer. Quantification of THG signal can accurately distinguish HER2-positive cells. Further analysis using Fourier transform infrared (FTIR) spectra reveals cancer-specific profiles, correlating lipid raft-corresponding spectra to THG signal, associating thus THG to chemical information.

    2. Autofluorescence flow sorting of breast cancer cell metabolism

      Amy T. Shah, Taylor M. Cannon, James N. Higginbotham, Robert J. Coffey and Melissa C. Skala

      Version of Record online: 12 OCT 2016 | DOI: 10.1002/jbio.201600128

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      Clinical cancer treatment aims to target all cell subpopulations within a tumor. Autofluorescence microscopy of the metabolic cofactors NAD(P)H and FAD has shown sensitivity to anti-cancer treatment response. Alternatively, flow cytometry is attractive for high throughput analysis and flow sorting. This study measures cellular autofluorescence in three flow cytometry channels and applies cellular autofluorescence to sort a heterogeneous mixture of breast cancer cells into subpopulations enriched for each phenotype.

    3. Remission spectrometry for blood vessel detection during stereotactic biopsy of brain tumors

      Niklas A. Markwardt, Herbert Stepp, Gerhard Franz, Ronald Sroka, Marcus Goetz, Petr Zelenkov and Adrian Rühm

      Version of Record online: 7 OCT 2016 | DOI: 10.1002/jbio.201600193

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      To increase the safety of stereotactic brain biopsies, it is crucial to avoid hemorrhages due to blood vessel rupture. By illuminating the tissue that is to be sampled and comparing the remitted intensities at two wavelengths with strongly differing hemoglobin absorption, blood vessels can be recognized before biopsy sampling. The method is easily implementable into a conventional biopsy needle and clearly discriminates between vessels within and outside the needle's coverage.

    4. Optical redox ratio and endogenous porphyrins in the detection of urinary bladder cancer: A patient biopsy analysis

      Scott Palmer, Karina Litvinova, Andrey Dunaev, Ji Yubo, David McGloin and Ghulam Nabi

      Version of Record online: 7 OCT 2016 | DOI: 10.1002/jbio.201600162

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      New avenues are sought for the early detection of urinary bladder cancer, a common cancer which is often missed during routine examination. One suggested technique is autofluorescence spectroscopy, however much is still unknown about autofluorescence properties in health and disease. We present new findings regarding differences in autofluorescence profiles between healthy and diseased tissue – reflecting alterations to biochemical processes – which may be of clinical worth for disease diagnosis.

    5. Structural damage of Bacillus subtilis biofilms using pulsed laser interaction with gold thin films

      Judith Krawinkel, Maria Leilani Torres-Mapa, Eisha Mhatre, Ákos T. Kovács and Alexander Heisterkamp

      Version of Record online: 7 OCT 2016 | DOI: 10.1002/jbio.201600146

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      Biofilms can have a negative impact in medical and industrial settings. Distinct changes on the surface structure and overall morphology are induced on two day-old B. subtilis biofilms using the interaction of 532 nm pulsed laser with gold thin films. After irradiation of biofilms in the presence of gold film, an increased number of dead bacterial cells was detected. Thus, this technique may be utilized in targeting and eradicating matured biofilms.

    6. Changes in endogenous UV fluorescence and biomechanical stiffness of bovine articular cartilage after collagenase digestion are strongly correlated

      William Lewis, Juan-Pablo Padilla-Martinez, Antonio Ortega-Martinez and Walfre Franco

      Version of Record online: 7 OCT 2016 | DOI: 10.1002/jbio.201600093

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      Articular cartilage contains a variety of fluorescent molecules, including constituents of collagen and other structural proteins. Ultraviolet fluorescence spectroscopy was carried out on bovine cartilage samples before and after digestion by collagenase, a model of osteoarthritis. Changes in the stiffness of the cartilage were found to correlate with changes in the UV fluorescence spectrum of the cartilage.

    7. Low-power laser irradiation in salivary glands reduces glycemia in streptozotocin-induced diabetic female rats

      Cíntia Yuki Fukuoka, Gabriella Torres Schröter, José Nicolau and Alyne Simões

      Version of Record online: 7 OCT 2016 | DOI: 10.1002/jbio.201600175

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      Low-power laser irradiation (LPLI) can modulate inflammation in vitro and in vivo. Diabetes results in chronic hyperglycemia and therefore chronic inflammation. Recent reports indicated that LPLI might have a systemic effect. Our study suggest that LPLI can reduce the glycemia, improving the insulin resistance, without changes in insulin levels, in a diabetic rat experimental model.

    8. Raman spectroscopic identification of Mycobacterium tuberculosis

      Stephan Stöckel, Susann Meisel, Björn Lorenz, Sandra Kloß, Sandra Henk, Stefan Dees, Elvira Richter, Sönke Andres, Matthias Merker, Ines Labugger, Petra Rösch and Jürgen Popp

      Version of Record online: 7 OCT 2016 | DOI: 10.1002/jbio.201600174

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      Tuberculosis has been a scourge of humankind from time immemorial and is still one of the world's most dangerous diseases. Thus, the per se cultivation-independent Raman microspectroscopy emerges as a perfect tool for a rapid on-the-spot mycobacterial diagnostic test. For the first time, it could be shown that a definite identification of mycobacterial species is possible by using Raman microspectroscopy on single bacterial cells in combination with elaborated statistical methods.

  4. Letters

    1. Direct and label-free detection of the human growth hormone in urine by an ultrasensitive bimodal waveguide biosensor

      Ana Belén González-Guerrero, Jesús Maldonado, Stefania Dante, Daniel Grajales and Laura M. Lechuga

      Version of Record online: 27 SEP 2016 | DOI: 10.1002/jbio.201600154

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      Graphical abstract of the hGH direct detection using a bimodal nanometric waveguide in which anti-hGH are immobilizing on by a silane coupling agent.

    2. Bessel-beam illumination in dual-axis confocal microscopy mitigates resolution degradation caused by refractive heterogeneities

      Ye Chen, Adam Glaser and Jonathan T. C. Liu

      Version of Record online: 26 SEP 2016 | DOI: 10.1002/jbio.201600196

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      The propagation-invariant and self-reconstructing features of Bessel beams have been shown to benefit volumetric microscopy of tissues with refractive heterogeneities. This study examined the resolution degradation that occurs when performing dual-axis confocal (DAC) microscopy within tissues using Gaussian or Bessel illumination. Results indicate Bessel beams are useful for preserving resolution in DAC microscopy, in which tissue-imaging performance is sensitive to positional changes and distortions of off-axis illumination and collection beams.

  5. Full Articles

    1. Evaluation of the 2-(1-Hexyloxyethyl)-2-devinyl pyropheophorbide (HPPH) mediated photodynamic therapy by macroscopic singlet oxygen modeling

      Rozhin Penjweini, Michele M. Kim, Baochang Liu and Timothy C. Zhu

      Version of Record online: 22 SEP 2016 | DOI: 10.1002/jbio.201600121

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      Concentration of the reacted singlet oxygen ([1O2]rx) can be calculated using singlet oxygen explicit dosimetry of light fluence (rate), initial tissue oxygenation, and photosensitizer concentration using a macroscopic model for HPPH-mediated photodynamic therapy (PDT) in radiation-induced fibrosarcoma (RIF) tumor model. Assessing tumor re-growth control across four dosimetric quantities (light fluence, photobleaching ratio, PDT dose, and [1O2]rx) in the RIF tumor model demonstrates that [1O2]rx is the most reliable dosimetric metric.

    2. Optimization and therapeutic effects of PDT mediated by ALA and MAL in the treatment of cutaneous malignant lesions: A comparative study

      Cassio Aparecido Lima, Viviane Pereira Goulart, Etelvino Jose Henriques Bechara, Luciana Correa and Denise Maria Zezell

      Version of Record online: 22 SEP 2016 | DOI: 10.1002/jbio.201600164

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      Topical photodynamic therapy (PDT) is a well succededed modality in the treatment of nonmelanoma skin cancers. Better cosmetic outcomes are expected irradiating the tissue in the optimized time. Thus, this study examined the optimum time-point to perform the light exposure after topical application of 5-aminolevulinic acid (ALA) and its methylated ester (MAL) in malignant lesions precursors of cutaneous squamous cell carcinoma. In addition, the therapeutic effects for both treatments were assessed after 10 and 20 days.

    3. The chemokines secretion and the oxidative stress are targets of low-level laser therapy in allergic lung inflammation

      Jorge Luis Costa Carvalho, Auriléia Aparecida de Brito, Ana Paula Ligeiro de Oliveira, Hugo Caire de Castro Faria Neto, Thiago Martini Pereira, Regiane Albertini de Carvalho, Elen Anatriello and Flávio Aimbire

      Version of Record online: 20 SEP 2016 | DOI: 10.1002/jbio.201600061

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      The manuscript goal is to provide evidence that low-level laser therapy (LLLT) applied non-invasively attenuates the intense eosinophilia as well as restore the balance of oxidative stress that are two of the most important features of allergic asthma. Taking it into consideration it is reasonable to suggest that laser treatment used for a long period can attenuate the inflammation in lung microenvironment allowing asthmatic patients to take lower doses of corticoid which, consequently result in fewer side-effects.

    4. Low-level laser therapy stimulates bone metabolism and inhibits root resorption during tooth movement in a rodent model

      Selly Sayuri Suzuki, Aguinaldo Silva Garcez, Hideo Suzuki, Edilson Ervolino, Won Moon and Martha Simões Ribeiro

      Version of Record online: 20 SEP 2016 | DOI: 10.1002/jbio.201600016

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      Orthodontic tooth movement is a complex biological process that induces periodontal tissue reactions of the supporting structures in response to biomechanical forces, leading to bone remodeling. Since tooth movement is based on a localized inflammatory effect, this study hypothesizes that low-level laser therapy can accelerate metabolic changes, influence the bone resorption and formation processes, and consequently result in a faster tooth movement with less damage to root surface.

    5. Photobiomodulation reduces abdominal adipose tissue inflammatory infiltrate of diet-induced obese and hyperglycemic mice

      Tania Mateus Yoshimura, Caetano Padial Sabino and Martha Simões Ribeiro

      Version of Record online: 16 SEP 2016 | DOI: 10.1002/jbio.201600088

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      Infra-red light application to adipose tissue reduces its inflammation and may be considered as a therapy to manage obesity-related diseases.