Journal of Biophotonics

Cover image for Vol. 8 Issue 4

Early View (Online Version of Record published before inclusion in an issue)

Editor: Jürgen Popp

Impact Factor: 3.856

ISI Journal Citation Reports © Ranking: 2013: 17/78 (BIOCHEMICAL RESEARCH METHODS); 17/74 (Biophysics)

Online ISSN: 1864-0648

Associated Title(s): Laser & Photonics Reviews

  1. Full Articles

    1. Hyperspectral imaging in medicine: image pre-processing problems and solutions in Matlab

      Robert Koprowski

      Article first published online: 9 FEB 2015 | DOI: 10.1002/jbio.201400133

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      A new application in Matlab intended for preliminary analysis of hyperspectral images. The available full source code and the description of the results obtained. Lack of licensing restrictions on the use and modification of the proposed application. Testing of the operation correctness on dozens of hyperspectral images.

    2. You have full text access to this OnlineOpen article
      Topographic prominence as a method for cluster identification in single-molecule localisation data

      Juliette Griffié, Lies Boelen, Garth Burn, Andrew P. Cope and Dylan M. Owen

      Article first published online: 6 FEB 2015 | DOI: 10.1002/jbio.201400127

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      The goal of this study is to present a new cluster analysis method adapted to single-molecule localisation microscopy, based on a topographic approach. By using the relative heights of the peaks in the local molecular density heat maps, cluster characteristics can be identified more accurately than with previously demonstrated height thresholds. The algorithm is validated using simulated and experimental data and found to significantly outperform previous cluster identification methods.

    3. Synergistic effects of atmospheric pressure plasma-emitted components on DNA oligomers: a Raman spectroscopic study

      Eugen Edengeiser, Jan-Wilm Lackmann, Erik Bründermann, Simon Schneider, Jan Benedikt, Julia E. Bandow and Martina Havenith

      Article first published online: 6 FEB 2015 | DOI: 10.1002/jbio.201400123

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      Plasma is a partially ionized gas containing free electrons, ions, and neutral particles. Recent developments in cold atmospheric-pressure plasmas have led to increased interest in their disinfecting properties. Despite an ongoing clinical evaluation, little is known about the mechanisms that lead to molecular modifications. The study aims to gain insight into inactivation mechanisms of plasmas. The focus is on the impact of plasma components of an indirect plasma on DNA oligomers in vitro.

    4. Photobleaching and phototoxicity of KillerRed in tumor spheroids induced by continuous wave and pulsed laser illumination

      Daria S. Kuznetsova, Marina V. Shirmanova, Varvara V. Dudenkova, Pavel V. Subochev, Ilya V. Turchin, Elena V. Zagaynova, Sergey A. Lukyanov, Boris E. Shakhov and Vladislav A. Kamensky

      Article first published online: 3 FEB 2015 | DOI: 10.1002/jbio.201400130

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      The article is devoted to study the photobleaching of the genetically encoded photosensitizer KillerRed. The work was performed on tumor spheroids upon different regimes of laser irradiation and included the analysis of fluorescence and mechanisms of cancer cell death after the treatment. These results can improve the understanding of the photobleaching mechanisms in phototoxic proteins and show the importance of appropriate selection of the treatment regime for PDT.

    5. Scheimpflug camera in the quantitative assessment of reproducibility of high-speed corneal deformation during intraocular pressure measurement

      Robert Koprowski, Renato Ambrósio Jr., Sven Reisdorf

      Article first published online: 26 JAN 2015 | DOI: 10.1002/jbio.201400137

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      Corvis ST (Oculus, Wetzlar, Germany) uses ultra-high-speed Scheimpflug photography during air-puff non-contact tonometry (NCT) that enables to measure intraocular pressure and dynamically evaluate corneal/ocular response. Many parameters derived from corneal deformation and vibration responses and ocular movement are calculated. Analysis of corneal deformation response with a frequency above 100 Hz is proposed using advanced image analysis and processing. The paper also presents the assessment of reproducibility of the performed measurements. It is a valuable contribution to the analysis of patients with diabetes, keratoconus or patients after surgery.

    6. A new approach for optical assessment of directional anisotropy in turbid media

      Pejhman Ghassemi, Lauren T. Moffatt, Jeffrey W. Shupp and Jessica C. Ramella-Roman

      Article first published online: 19 JAN 2015 | DOI: 10.1002/jbio.201400124

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      A study of polarized light transport in scattering media exhibiting directional anisotropy or linear birefringence is presented. Novel theoretical and experimental methodologies for the quantification of birefringent alignment based on out-of-plane polarized light transport are demonstrated. A polarized Monte Carlo model and a polarimetric imaging system were devised to predict and measure the impact of birefringence on an impinging linearly polarized light beam. Ex-vivo experiments conducted on bovine tendon showed good agreement with the analytical results.

    7. GJIC Enhances the phototoxicity of photofrin-mediated photodynamic treatment by the mechanisms related with ROS and Calcium pathways

      Dengpan Wu, Lixia Fan, Chengfang Xu, Zhen Liu, Yuan Zhang, Lucy Liu, Qin Wang, Liang Tao

      Article first published online: 19 JAN 2015 | DOI: 10.1002/jbio.201400131

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      Despite initially positive responses, recurrences after Photodynamic treatment (PDT) can occur and there is need for improvement in the effectiveness of PDT. It is shown that the presence of GJIC (Connexin32) increased the PDT phototoxicity in cell and animal level, which was related with ROS and calcium pathways. The study indicates the possibility that up-regulation or maintenance of GJ functionality may be used to increase the efficacy of PDT.

    8. Differential remodeling of extracellular matrices by breast cancer initiating cells

      Anju M. Raja, Shuoyu Xu, Shuangmu Zhuo, Dean C.S. Tai, Wanxin Sun, Peter T.C. So, Roy E. Welsch, Chien-Shing Chen and Hanry Yu

      Article first published online: 19 JAN 2015 | DOI: 10.1002/jbio.201400079

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      The extracellular matrix-remodeling effects of cancer initiating cells (CICs) using Second Harmonic Generation (SHG) microscopy is studied to identify potential phenotypic signatures of the CIC-rich tumors. The CIC-rich tumors have less collagen in the tumor interior than in the CIC-poor tumors and collagen fibers are more preferentially aligned perpendicular to the CIC-rich tumor boundary, suggesting more invasive behavior and more effective collagen remodeling capability of the CIC-rich tumors.

    9. Photodamage in deep tissue two-photon optical biopsy of human skin

      Luca Dalbosco, Giulia Zanini, Elvira D'Amato, Francesco Tessarolo, Sebastiana Boi, Paolo Bauer, Albrecht Haase and Renzo Antolini

      Article first published online: 18 DEC 2014 | DOI: 10.1002/jbio.201400083

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      Photodamage was studied in thick samples of human skin tissue (healthy skin and neoplastic lesions). Photobleaching, photoionization, and thermomechanical damage effects were characterized comparatively. The laser power dependence of the damage rates allowed to connect macroscopic effects to underlying molecular processes. Tissue alterations were found only from thermomechanical cavitation and limited to superficial layers of the epidermis. From the depth-dependencies of all damage thresholds a depth-dependent power-compensation scheme was defined allowing for damage-free deep tissue optical biopsy.

    10. Clinical assessment of non carious cervical lesion using swept-source optical coherence tomography

      Ikumi Wada, Yasushi Shimada, Masaomi Ikeda, Alireza Sadr, Syozi Nakashima, Junji Tagami and Yasunori Sumi

      Article first published online: 11 DEC 2014 | DOI: 10.1002/jbio.201400113

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      Non-carious cervical lesions (NCCLs) is a dental hard tissue defect that is unrelated to dental caries. We examined the incidence of occlusal wear and cervical demineralization in relation to NCCL dimensions using SS-OCT. Cervival demineralization was observed in 56.2% of small NCCLs, and the ratio increased to >80% in larger NCCLs. Cervical demineralization and occlusal attrition can be considered as an etiological factor in formation and progress of NCCLs.

    11. Molecularly imprinted hollow sphere array for the sensing of proteins

      Wei Chen, Min Xue, Kenneth J. Shea, Zihui Meng, Zequn Yan, Zhe Wang, Fei Xue, Feng Qu

      Article first published online: 11 DEC 2014 | DOI: 10.1002/jbio.201400100

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      A new type of hollow sphere array for the label-free detection of hemoglobin (Hb) was developed. The molecularly imprinted hollow sphere array (MIHSA) with 3D photonic structure display remarkable selectivity and adsorption capacity to Hb. Furthermore, the reflection of the MIHSA red shifted significantly in response to Hb with obviously color change. The MIHSA showed promising potential for the naked-eye detection of target Hb.

  2. Review Articles

    1. Holographic imaging of unlabelled sperm cells for semen analysis: a review

      Giuseppe Di Caprio, Maria Antonietta Ferrara, Lisa Miccio, Francesco Merola, Pasquale Memmolo, Pietro Ferraro and Giuseppe Coppola

      Article first published online: 9 DEC 2014 | DOI: 10.1002/jbio.201400093

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      Semen analysis has long represented the standard test for evaluating male fertility. Though still useful, the test is not perfect, as it fails to accurately predict fertility status in certain situations. This paper proposes a review of analysis on sperm properties based on Digital Holography. The addition of the third dimension provides a better statistic useful to relate the sperm anomalies with male infertility and to enable cell sorting.

  3. Full Articles

    1. Low-level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

      Felipe F. Sperandio, Alyne Simões, Luciana Corrêa, Ana Cecília C. Aranha, Fernanda S. Giudice, Michael R. Hamblin and Suzana C.O.M. Sousa

      Article first published online: 20 NOV 2014 | DOI: 10.1002/jbio.201400064

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      Low-level laser therapy accelerates the epithelium maturation during wound healing; this picture illustrates how the immunohistochemical expression of cytokeratin 10, a terminal differentiation biomarker, is significantly augmented in the keratinocytes of the laser-irradiated lesion if compared to the control group. The center of the wound (CW) shows complete re-epithelialization; the covering epithelium over the connective tissue (CT) notably expresses cytokeratin 10.

    2. Dye distance mapping using waveguide evanescent field fluorescence microscopy and its application to cell biology

      Frederik Fleissner, Michael Morawitz, S. Jeffrey Dixon, Uwe Langbein and Silvia Mittler

      Article first published online: 17 NOV 2014 | DOI: 10.1002/jbio.201400088

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      A microscopy method is described enabling to measure not only distances between an adhesion point of a cell and its substratum at particular locations but to map the distance of the cell's plasma membrane along an entire cell or even along an entire cell population, allowing distinguishing between the various adhesion forms and imaging membrane bending in-between adhesion sites giving more insides into the cell-substratum interaction.

    3. Detection and controlled depletion of cancer cells using photothermal phase microscopy

      Nir Abraham Turko, Itay Barnea, Omry Blum, Rafi Korenstein and Natan Tzvi Shaked

      Article first published online: 17 NOV 2014 | DOI: 10.1002/jbio.201400095

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      Laser therapy for real-time, selective cancer treatment is now one step closer to realization, as a new method for ablating cancer cells specifically is implemented. The system is comprised of a photothermal phase interferometer, which can quantitatively image all cells in an in vitro sample, labeled and unlabeled, and then selectively choose cells for ablation using novel image contrast mechanism that is based on the heat signatures of gold nanoparticles.

    4. Light-emitting diode therapy in exercise-trained mice increases muscle performance, cytochrome c oxidase activity, ATP and cell proliferation

      Cleber Ferraresi, Nivaldo Antonio Parizotto, Marcelo Victor Pires de Sousa, Beatriz Kaippert, Ying-Ying Huang, Tomoharu Koiso, Vanderlei Salvador Bagnato and Michael R. Hamblin

      Article first published online: 6 NOV 2014 | DOI: 10.1002/jbio.201400087

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      Light-emitting diode therapy (LEDT) applied over the leg, gluteus and lower-back muscles of mice using a LED cluster increased muscle performance, ATP and mitochondrial metabolism; oxidative stress and proliferative myocyte markers in mice subjected to acute and progressive strength training. Six bi-daily training sessions LEDT-After and LEDT-Before-After regimens more than doubled muscle performance and increased ATP more than tenfold. The effectiveness of LEDT on improving muscle performance and recovery suggest applicability for high performance sports and in training programs.

    5. Multimodal and multiplex spectral imaging of rat cornea ex vivo using a white-light laser source

      Hiroki Segawa, Yuichi Kaji, Philippe Leproux, Vincent Couderc, Takeaki Ozawa, Tetsuro Oshika and Hideaki Kano

      Article first published online: 6 NOV 2014 | DOI: 10.1002/jbio.201400059

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      Multimodal nonlinear spectral imaging with coherent anti-Stokes Raman scattering (CARS) molecular fingerprinting was applied to ex vivo measurement of rat cornea. Complicated inner structure of the cornea was clearly visualized with molecular structural information produced by combined spectral analysis of multiplex CARS, second harmonic generation (SHG) and multiplex third-order sum frequency generation (TSFG). This study will open the door for molecular level analysis in corneal medical diagnostics.

    6. Polarization second harmonic generation microscopy provides quantitative enhanced molecular specificity for tissue diagnostics

      Rajesh Kumar, Kirsten M. Grønhaug, Elisabeth I. Romijn, Andreas Finnøy, Catharina L. Davies, Jon O. Drogset and Magnus B. Lilledahl

      Article first published online: 3 NOV 2014 | DOI: 10.1002/jbio.201400086

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      Polarization dependent effects introduced by optics present in a commercial microscope can create artifact in susceptibility (χ) image, which ultimately may lead to misleading results. This report discusses the issues responsible for image artifacts in p-SHG analysis and provides the relevant solutions in terms of calibrations. Moreover, as a proof-of-concept, χ-parameters value of a heart muscle, ovary tissue and osteoarthritic cartilage was obtained by p-SHG analysis.

    7. In-vitro investigations on laser-induced smoke generation mimicking the laparoscopic laser surgery purposes

      Wael Y. Khoder, Christian G. Stief, Sebastian Fiedler, Thomas Pongratz, Wolfgang Beyer, Georg Hennig, Adrian Rühm and Ronald Sroka

      Article first published online: 3 NOV 2014 | DOI: 10.1002/jbio.201400061

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      Intraoperative smoke generation is an adherent phenomenon to laser-assisted surgery that could obscure surgical-vision. An experimental setup mimicking clinical laparoscopy is used to detect smoke-production. This time dependent signal correlates to the ablation-rate. Smoke generation depends on the optical properties of tissue and thus on the laser wavelength used for ablation. Laparoscopic surgeons should be aware of specific wave-length-laser-tissue interaction, avoiding carbonization during cutting/coagulation to reduce smoke-generation yielding visual advantages.

    8. The use of optical trap and microbeam to investigate the mechanical and transport characteristics of tunneling nanotubes in tumor spheroids

      Pooja Patheja, Raktim Dasgupta, Alok Dube, Sunita Ahlawat, Ravi Shanker Verma and Pradeep Kumar Gupta

      Article first published online: 29 OCT 2014 | DOI: 10.1002/jbio.201400039

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      The use of optical trap and microbeam for investigating mechanical and transport properties of inter cellular tunneling nanotubes (TnTs) in tumor spheroids has been demonstrated. TnTs in tumor spheroids have been visualized by manipulating TnT connected cells using optical tweezers. Functionality of the TnTs for transferring cytoplasmic vesicles and injected dye molecules by optoporation method has been studied. Further, the TnTs could be longitudinally stretched by manipulating the connected cells and their elastic response was studied.

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