Journal of Biophotonics

Cover image for Vol. 9 Issue 5

Early View (Online Version of Record published before inclusion in an issue)

Editor: Jürgen Popp

Impact Factor: 4.447

ISI Journal Citation Reports © Ranking: 2014: 7/87 (Optics); 13/79 (BIOCHEMICAL RESEARCH METHODS); 13/73 (Biophysics)

Online ISSN: 1864-0648

Associated Title(s): Laser & Photonics Reviews

  1. Full Articles

    1. Multispectral optoacoustic and MRI coregistration for molecular imaging of orthotopic model of human glioblastoma

      Amalina Binte Ebrahim Attia, Chris Jun Hui Ho, Prashant Chandrasekharan, Ghayathri Balasundaram, Hui Chien Tay, Neal C. Burton, Kai-Hsiang Chuang, Vasilis Ntziachristos and Malini Olivo

      Version of Record online: 19 APR 2016 | DOI: 10.1002/jbio.201500321

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      Multimodal imaging can be used to study various tissue parameters in cancer and gain insights into tumor physiology. This work explores the feasibility of using multimodal MRI/optoacoustic image coregistration and hybrid imaging for non-invasive molecular imaging of glucose uptake in an orthotopic glioblastoma mouse model. Analogous to PET/CT and PET/MRI configurations, MSOT/MRI imaging can be used to acquire coregistered molecular/functional/anatomical information, thereby improving tumor detection and characterization.

    2. Complex refractive index of normal and malignant human colorectal tissue in the visible and near-infrared

      Panagiotis Giannios, Spyridon Koutsoumpos, Konstantinos G. Toutouzas, Maria Matiatou, George C. Zografos and Konstantinos Moutzouris

      Version of Record online: 19 APR 2016 | DOI: 10.1002/jbio.201600001

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      Multi-wavelength prism coupling refractometry is employed for the ex-vivo reflectance measurement of freshly excised human colorectal specimens. Novel data of the complex refractive index of human intestinal tissue are provided and the index contrast between colorectal tumors and healthy mucosa is demonstrated as a marker of disease, suggesting its potential for novel diagnostic tools in endoscopy.

    3. Label-free in vivo cellular-level detection and imaging of apoptosis

      Andrew J. Bower, Marina Marjanovic, Youbo Zhao, Joanne Li, Eric J. Chaney and Stephen A. Boppart

      Version of Record online: 19 APR 2016 | DOI: 10.1002/jbio.201600003

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      Apoptosis, or programmed cell death plays a critical role in health and homeostasis as well as in the treatment of many diseases. While this process has been characterized extensively in vitro, label-free detection of cells in vivo has yet to be shown. In this study, fluorescence lifetime imaging microscopy of reduced nicotinamide adenine dinucleotide is utilized to assess the metabolic response of in vivo mouse keratinocytes following induction of apoptosis.

    4. A novel multiphoton microscopy images segmentation method based on superpixel and watershed

      Weilin Wu, Jinyong Lin, Shu Wang, Yan Li, Mingyu Liu, Gaoqiang Liu, Jianyong Cai, Guannan Chen and Rong Chen

      Version of Record online: 19 APR 2016 | DOI: 10.1002/jbio.201600007

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      Processing procedure of the images segmentation method based on superpixels and watershed (MSW) segmentation algorithm for multiphoton microscopy image.

    5. Induction of neuronal activation by femtosecond-pulsed laser irradiation and its potential application for amyloid-β–induced toxicity assessment

      Seunghee Lee, Jonghee Yoon, Myunghwan Choi and Chulhee Choi

      Version of Record online: 19 APR 2016 | DOI: 10.1002/jbio.201600004

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      Direct optical stimulation at a neuron using femtosecond-pulsed laser can induce calcium elevation and ROS generation mediated by superoxide. We verified the applicability of such mechanism for the assessment of neuronal functionality in vehicle and Aβ-treated neurons. This work suggests promising applications of the potential for implementing such laser-induced neural activation for rapid and reliable drug screening.

    6. Photomodulation of the osteoclastogenic potential of oral squamous carcinoma cells

      Tatiana Dias Schalch, Kristianne Porta Santos Fernandes, João Costa-Rodrigues, Mônica Pereira Garcia, Raquel Agnelli Mesquita-Ferrari, Sandra Kalil Bussadori and Maria Helena Fernandes

      Version of Record online: 18 APR 2016 | DOI: 10.1002/jbio.201500292

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      This study evaluated whether photobiomodulation (PBM), using the parameters indicated for the treatment of mucositis, could modulate the osteoclastogenic potential of cells derived from oral squamous cell carcinoma (SCC). PBM (4 J/cm2) decreased the pro-osteoclastogenic potential of these cells. The safe use of PBM for the treatment of oral mucositis depends on a full understanding of the effects of laser irradiation on the different metabolic pathways of oral SCC.

    7. SPR-based plastic optical fibre biosensor for the detection of C-reactive protein in serum

      Ayda Aray, Francesco Chiavaioli, Mojtaba Arjmand, Cosimo Trono, Sara Tombelli, Ambra Giannetti, Nunzio Cennamo, Mahmood Soltanolkotabi, Luigi Zeni and Francesco Baldini

      Version of Record online: 18 APR 2016 | DOI: 10.1002/jbio.201500315

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      A surface plasmon resonance-based biosensor implemented on a plastic optical fibre for the selective detection of C-reactive protein in a real and complex matrix (i.e. serum) is presented. With detection limit of 9 µg L–1 (below that requested in clinical applications), the proposed biosensing platform, integrated into a home-made thermo-stabilized flow cell, could underpin the need for high sensitive, real-time, label-free, portable and low cost platforms for future lab-on-a-chip applications.

    8. Investigating fibroblast cells under “safe” and “injurious” blue-light exposure by holographic microscopy

      Alejandro Calabuig, Martina Mugnano, Lisa Miccio, Simonetta Grilli and Pietro Ferraro

      Version of Record online: 18 APR 2016 | DOI: 10.1002/jbio.201500340

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      A study on the temporal evolution of cell morphology and volume during blue light exposure is reported. The results reveal a behaviour that is typical of necrotic cells, with early swelling and successive leakage of the intracellular liquids when the laser is set in the “injurious” operation. The approach may open the route to a deep investigation of light-cell interactions, with information about death pathways and threshold conditions between healthy and damaged cells when subjected to light-exposure.

    9. Differentiating responses of lung cancer cell lines to Doxorubicin exposure: in vitro Raman micro spectroscopy, oxidative stress and bcl-2 protein expression

      Zeineb Farhane, Franck Bonnier, Marcus Alexander Maher, Jane Bryant, Alan Casey and Hugh James Byrne

      Version of Record online: 18 APR 2016 | DOI: 10.1002/jbio.201600019

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      Raman microspectroscopy is employed to investigate and compare the in vitro mechanisms of action of Doxorubicin and cellular resistances of cancer cell lines A549 and Calu-1.

      Results show the potential of Raman not only to distinguish the different mechanisms of action and subcellular response but also to elucidate drug resistance in the different cell lines.

  2. Letters

    1. Chemico-mechanical imaging of Barrett's oesophagus

      Francesca Palombo, Marco Madami, Daniele Fioretto, Jayakrupakar Nallala, Hugh Barr, Antoine David and Nick Stone

      Version of Record online: 18 APR 2016 | DOI: 10.1002/jbio.201600038

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      Barrett's oesophagus is a condition characterized by a change in the lining of the oesophagus that markedly increases the risk of adenocarcinoma. Here, the first site-matched application of Brillouin microscopy, Raman microscopy and FTIR micro-spectroscopic imaging to ex-vivo epithelial tissue – Barrett's oesophagus is demonstrated. Brillouin microscopy enables maps of elasticity of the epithelium to be obtained, whilst Raman and FTIR imaging provide ‘chemical images' without the need for labelling or staining. This approach provides a valuable platform for investigating the structure, biomechanics and composition of complex heterogeneous systems.

  3. Full Articles

    1. Activity assessment of root caries lesions with thermal and near-IR imaging methods

      Robert C. Lee, Cynthia L. Darling, Michal Staninec, Antonio Ragadio and Daniel Fried

      Version of Record online: 6 APR 2016 | DOI: 10.1002/jbio.201500333

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      The highly mineralized surface layer plays an important role in the dehydration process of remineralized dentin lesions. The PS-OCT algorithm for the automated assessment of remineralization successfully detected the highly mineralized surface layer on root caries lesions. Thermal imaging provided the best performance in root caries lesion activity assessment. Detection of remineralization with these novel methods has the potential to aid in the clinical assessment of root caries lesion activity.

    2. Bladder cancer diagnosis from bladder wash by Fourier transform infrared spectroscopy as a novel test for tumor recurrence

      Seher Gok, Ozge Z. Aydin, Yavuz S. Sural, Ferruh Zorlu, Umit Bayol and Feride Severcan

      Version of Record online: 4 APR 2016 | DOI: 10.1002/jbio.201500322

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      Cystoscopy combined with urine cytology, is a gold standard in the detection of the bladder tumor. Cystoscopy is an invasive technique while the cytology has low sensitivity. Infrared data coupled with chemometrics offer a non-invasive, observer-independent and low cost test, for the detection of bladder tumor recurrence from bladder wash samples that are routinely collected during cystoscopy.

    3. Synthesis of a poly(ethylene glycol) galloyl sensitizer tip for an ’all-in-one' photodynamic device

      Ashwini A. Ghogare and Alexander Greer

      Version of Record online: 4 APR 2016 | DOI: 10.1002/jbio.201600013

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      This paper describes the synthesis of a specialized silica tip for an optical fiber device capable of delivering all components necessary for photodynamic therapy. The hydrolytic stability of the attached PEGs and the extent to which the PEG groups enhance solubility will also be discussed. The new triPEG-galloyl sensitizer has the potential for use in intraoperative pointsource photodynamic therapy which aims for precision treatment of residual disease.

    4. You have full text access to this OnlineOpen article
      Cell nuclei have lower refractive index and mass density than cytoplasm

      Mirjam Schürmann, Jana Scholze, Paul Müller, Jochen Guck and Chii J. Chan

      Version of Record online: 24 MAR 2016 | DOI: 10.1002/jbio.201500273

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      Digital holographic microscopy is used to determine the refractive index (RI) and dry mass of isolated nuclei. Nuclear RI is found to be lower compared to whole cells in four cell lines. Changes to nuclear volume by electrostatic and osmotic conditions, and chromatin modifications, reveal a linear relationship between RI and mass density. These findings indicate a lower nuclear density compared to the cytoplasm also in the in situ situation.

    5. Non-invasive optical assessment of viscosity of middle ear effusions in otitis media

      Guillermo L. Monroy, Paritosh Pande, Ryan L. Shelton, Ryan M. Nolan, Darold R. Spillman Jr., Ryan G. Porter, Michael A. Novak and Stephen A. Boppart

      Version of Record online: 24 MAR 2016 | DOI: 10.1002/jbio.201500313

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      Middle ear disease is commonly evaluated with otoscopy, providing limited qualitative information about the tympanic membrane and middle-ear content. Here a method is presented to assess the general state of the middle ear with cross-sectional optical coherence tomography images that directly reveal the presence of a scattering effusion. Relative Stokes-Einstein coefficients are calculated for various suspensions and phantoms and both in vivo and ex vivo human middle ear effusion samples.

  4. Review Articles

    1. Photodynamic therapy in the upper aerodigestive tract. Overview and outlook

      Veronika Volgger and Christian Stephan Betz

      Version of Record online: 24 MAR 2016 | DOI: 10.1002/jbio.201600036

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      Down to the present day, photodynamic therapy (PDT) is rarely used in the treatment of head and neck malignancies. However, clinical trials on PDT treatment of early and advanced head and neck squamous cell carcinomas, field cancerization and “difficult-to-treat” basal cell carcinomas show promising results. This article is aimed to give a comprehensive update on the current role of PDT in the head and neck and discuss further challenges and developments.

  5. Full Articles

    1. Comparing Raman and fluorescence lifetime spectroscopy from human atherosclerotic lesions using a bimodal probe

      Sebastian Dochow, Hussain Fatakdawala, Jennifer E. Phipps, Dinglong Ma, Thomas Bocklitz, Michael Schmitt, John W. Bishop, Kenneth B. Margulies, Laura Marcu and Jürgen Popp

      Version of Record online: 22 MAR 2016 | DOI: 10.1002/jbio.201500341

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      Fluorescence lifetime imaging (FLIM) and Raman are two promising methods to support morphological intravascular imaging techniques with chemical contrast. Both approaches may support each other or might be used in combination with OCT / IVUS for morphological and chemical assessment of intravascular plaques. In this contribution both modalities were simultaneously acquired from two human coronary specimens using a bimodal probe.

    2. Improving the quantitative accuracy of cerebral oxygen saturation in monitoring the injured brain using atlas based Near Infrared Spectroscopy models

      Michael Clancy, Antonio Belli, David Davies, Samuel J. E. Lucas, Zhangjie Su and Hamid Dehghani

      Version of Record online: 22 MAR 2016 | DOI: 10.1002/jbio.201500302

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      Utilisation of 3D Atlas models and localised high density NIR spectroscopic measurements improves the quantitative accuracy of recovered baseline measurements, such as cerebral oxygenation.

    3. Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy

      Molin Wongwattanakul, Chariya Hahnvajanawong, Patcharaporn Tippayawat, Sirinart Chio-Srichan, Chanvit Leelayuwat, Temduang Limpaiboon, Patcharee Jearanaikoon and Philip Heraud

      Version of Record online: 21 MAR 2016 | DOI: 10.1002/jbio.201500253

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      Cholangiocarcinoma (CCA), a cancer of bile duct epithelium, is a major health problem in Thailand especially in the northeast. Overall treatment outcomes have not shown much improvement because the disease is usually detected at an advanced stage and often shows chemotherapeutic resistance. High-throughput Fourier Transform Infrared (FTIR) microspectroscopy can be used for cell classification and has the potential to diagnose cancer and possibly predict chemo-response. This is the first study to demonstrate the use of FTIR microspectroscopy to assess chemo-response both in vitro and in vivo.

    4. Four-dimensional live imaging of hemodynamics in mammalian embryonic heart with Doppler optical coherence tomography

      Shang Wang, David S. Lakomy, Monica D. Garcia, Andrew L. Lopez III, Kirill V. Larin and Irina V. Larina

      Version of Record online: 21 MAR 2016 | DOI: 10.1002/jbio.201500314

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      Congenital heart disease is the most common congenital disorder, affecting 1% newborns in the U.S. Visualization and measurement of hemodynamics in early developing mammalian heart can provide great insights for improved understanding of normal cardiogenesis and congenital cardiac defects. In this study, Doppler optical coherence tomography is performed in live mouse embryo culture to obtain the first four-dimensional high-resolution imaging and measurement of hemodynamic features in the mammalian embryonic heart.