Photodamage in deep tissue two-photon optical biopsy of human skin
Luca Dalbosco, Giulia Zanini, Elvira D'Amato, Francesco Tessarolo, Sebastiana Boi, Paolo Bauer, Albrecht Haase and Renzo Antolini
Article first published online: 18 DEC 2014 | DOI: 10.1002/jbio.201400083
Photodamage was studied in thick samples of human skin tissue (healthy skin and neoplastic lesions). Photobleaching, photoionization, and thermomechanical damage effects were characterized comparatively. The laser power dependence of the damage rates allowed to connect macroscopic effects to underlying molecular processes. Tissue alterations were found only from thermomechanical cavitation and limited to superficial layers of the epidermis. From the depth-dependencies of all damage thresholds a depth-dependent power-compensation scheme was defined allowing for damage-free deep tissue optical biopsy.