Journal of Biophotonics

Cover image for Vol. 10 Issue 8

Early View (Online Version of Record published before inclusion in an issue)

Editor: Jürgen Popp

Impact Factor: 4.328

ISI Journal Citation Reports © Ranking: 2016: 10/92 (Optics); 12/77 (BIOCHEMICAL RESEARCH METHODS); 15/72 (Biophysics)

Online ISSN: 1864-0648

Associated Title(s): Laser & Photonics Reviews


    1. Enhanced volumetric imaging in 2-photon microscopy via acoustic lens beam shaping

      Simonluca Piazza, Paolo Bianchini, Colin Sheppard, Alberto Diaspro and Martí Duocastella

      Version of Record online: 25 JUL 2017 | DOI: 10.1002/jbio.201700050

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      A simple technique for fast volumetric imaging and enhanced signal-to-background (S/B) in 2-photon microscopy is presented. The approach uses an acoustic optofluidic lens to shape the excitation beam on a pixel-by-pixel basis. Control of the phase induced by the lens enables both fast axial focus scanning, for continuous volumetric imaging, and controlled aberrated imaging, suitable for background removal. The high temporal resolution and enhanced penetration depth capabilities of the method are demonstrated by imaging mouse brain tissue.

  2. Full Articles

    1. Imaging blood flow inside highly scattering media using ultrasound modulated optical tomography

      Altaf Hussain, Wiendelt Steenbergen and Ivo M. Vellekoop

      Version of Record online: 6 JUL 2017 | DOI: 10.1002/jbio.201700013

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      Sensitive, accurate and noninvasive method to measure blood flow is important to study biological processes such as local metabolism, pharmacodynamics, and stroke recovery. Optical methods of flowmetry possess these features, however the lack of spatial resolution with increasing depth due multiple scattering present challenge. Ultrasound modulated optical tomography (UOT), a hybrid of light and ultrasound combines the high sensitivity to blood flow (i. e. attribute of the optical techniques) and the spatial resolution (i. e. the attribute of ultrasound). The ability of UOT for depth resolved blood flow imaging is illustrated in the image above.

    2. Guiding cellular activity with polarized light

      Colin Constant, Andrea Bergano, Kiminobu Sugaya and Aristide Dogariu

      Version of Record online: 3 JUL 2017 | DOI: 10.1002/jbio.201600326

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      Polarized light can exert a force on dielectric materials causing them to align in the direction of polarization. We show here that the activity of the sub-cellular component, actin, responsible for cellular motion, is also affected by the action of polarized light. Using statistically robust methods for image analysis, we demonstrate that preferred actin motion is directed along the direction of light polarization, indicating possible control over motion of the entire cell.

    3. Towards the use of bioresorbable fibers in time-domain diffuse optics

      Laura Di Sieno, Nadia G. Boetti, Alberto Dalla Mora, Diego Pugliese, Andrea Farina, Sanathana Konugolu Venkata Sekar, Edoardo Ceci-Ginistrelli, Davide Janner, Antonio Pifferi and Daniel Milanese

      Version of Record online: 21 JUN 2017 | DOI: 10.1002/jbio.201600275

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      In this work, we add a step toward a new way to look inside the body using bioresorbable optical fibers that can be implanted in patients. We described their fabrication and we demonstrate their suitability in retrieving optical properties (related to tissue components and structure). Bioresorbable fibers respond to the need of low cost and minimally invasive instruments for diagnostics (e. g. follow-up after surgical interventions) or treatments (e. g. photodynamic therapy).

    4. High sensitivity non-invasive detection of calcifications deep inside biological tissue using Transmission Raman Spectroscopy

      Adrian Ghita, Pavel Matousek and Nick Stone

      Version of Record online: 21 JUN 2017 | DOI: 10.1002/jbio.201600260

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      An advanced Transmission Raman Spectroscopy (TRS) system has been developed to characterise the chemical composition of calcifications buried deep inside tissue. The calcifications were detected at clinically relevant concentrations in ∼40 mm-thick phantom tissue. The research represents a key step towards a future breast cancer diagnostic tool for distinguishing in vivo malignant and benign lesions from the chemical makeup of associated calcifications.

    5. Cetuximab-conjugated nanodiamonds drug delivery system for enhanced targeting therapy and 3D Raman imaging

      Dandan Li, Xin Chen, Hong Wang, Jie Liu, Meiling Zheng, Yang Fu, Yuan Yu and Jinfang Zhi

      Version of Record online: 21 JUN 2017 | DOI: 10.1002/jbio.201700011

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      Cetuximab-nanodiamonds (NDs)-cisplatin targeting drug delivery system is developed in this work, which combines specific targeting and enhanced therapeutic efficacy capabilities, incomparison with nonspecific NDs-cisplatin conjugate and specific EGF-NDs-cisplatin bioconjugate. Besides, the three-dimensional Raman imaging technique is utilized to visualize the performance of NDs-based drug delivery system in cancer cell targeting and internalization. These advantageous properties of cetuximab-NDs-cisplatin propose a promising treatment and imaging tool for further clinical application.

    6. Development of a classification model for non-alcoholic steatohepatitis (NASH) using confocal Raman micro-spectroscopy

      Jie Yan, Yang Yu, Jeon Woong Kang, Zhi Yang Tam, Shuoyu Xu, Eliza Li Shan Fong, Surya Pratap Singh, Ziwei Song, Lisa Tucker-Kellogg, Peter T. C. So and Hanry Yu

      Version of Record online: 21 JUN 2017 | DOI: 10.1002/jbio.201600303

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      Raman micro-spectroscopy was used to detect and quantify NASH signatures on mice model tissue samples. Quantification of the signatures such as lipid content, using spectrum decomposition and machine learning techniques, revealed their spatiotemporal redistribution as the disease progresses. We identified biochemical changes specific to NASH and show that the classification model could accurately detect NASH (AUC=0.85–0.87). This model can be further validated in clinical samples.

  3. Letters

    1. Diffractive corneal inlay for presbyopia

      Walter D. Furlan, Salvador García-Delpech, Patricia Udaondo, Laura Remón, Vicente Ferrando and Juan A. Monsoriu

      Version of Record online: 21 JUN 2017 | DOI: 10.1002/jbio.201600320

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      A new concept of corneal inlay, for the treatment of presbyopia, is proposed. In it, the micro-holes on the inlay surface, that are essential to allow the flow of nutrients, are arranged to take advantage of the unwanted diffracted light in previous small aperture inlays, for creating a true near focus. The inlay design can be customized. Experimental results demonstrate the high performance of this proposal in comparison with current designs.

  4. Full Articles

    1. Ratiometric analysis of optical coherence tomography–measured in vivo retinal layer thicknesses for the detection of early diabetic retinopathy

      Basanta Bhaduri, Ryan L. Shelton, Ryan M. Nolan, Lucas Hendren, Alexandra Almasov, Leanne T. Labriola and Stephen A. Boppart

      Version of Record online: 21 JUN 2017 | DOI: 10.1002/jbio.201600282

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      Six retinal layer boundaries were segmented from spectral-domain optical coherence tomography (SD-OCT) images using open-source software. Five study groups were identified and one-way analyses of variance (ANOVA) were performed between the adjacent study groups. ANOVA indicated differences in thicknesses and their ratios among the study groups. Ratiometric analysis may provide more sensitive parameters for detecting changes in diabetic mellitus (DM) and diabetic retinopathy (DR).

    2. Propagation of Gaussian and Laguerre-Gaussian vortex beams through mouse brain tissue

      Lingyan Shi, Lukas Lindwasser, Wubao Wang, Robert Alfano and Adrián Rodríguez-Contreras

      Version of Record online: 21 JUN 2017 | DOI: 10.1002/jbio.201700022

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      To test the hypothesis that applying Laguerre-Gaussian (LG) vortex beam would obtain higher penetration depth than Gaussian (G) beam for brain imaging, light transmittances of LG beams in mouse brain tissue were measured at different topological charges. Study showed no obvious difference between the transmittances of LG and G beams, which may be due to the interference effects in brain tissue that are not yet fully understood.

    3. Doxorubicin kinetics and effects on lung cancer cell lines using in vitro Raman micro-spectroscopy: binding signatures, drug resistance and DNA repair

      Zeineb Farhane, Franck Bonnier, Orla Howe, Alan Casey and Hugh J. Byrne

      Version of Record online: 21 JUN 2017 | DOI: 10.1002/jbio.201700060

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      Raman micro-spectroscopy is employed to investigate and compare Doxorubicin mechanisms of action, its kinetics and different cellular resistances of cancer cell lines A549 and Calu-1. Results show the potential of Raman not only to distinguish the different mechanisms of action at subcellular level but also to elucidate drug resistance by increase tolerance to DNA damage and higher DNA repair.

    4. High–resolution deep functional imaging of the whole mouse brain by photoacoustic computed tomography in vivo

      Pengfei Zhang, Lei Li, Li Lin, Peng Hu, Junhui Shi, Yun He, Liren Zhu, Yong Zhou and Lihong V. Wang

      Version of Record online: 21 JUN 2017 | DOI: 10.1002/jbio.201700024

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      Aim and scope: To reveal the mechanism of brain disorders, an imaging methodology with increased spatial and temporal resolution as well as deep penetration in tissues is highly required. We report a photoacoustic computed tomography (PACT) system for mapping the microvascular network of a whole mouse brain with the skull intact. We then performed functional imaging and observed strong interhemispherical correlations between functional regions and the epileptic wave propagating in the deep brain.

    5. Fs-laser ablation of teeth is temperature limited and provides information about the ablated components

      Rebeca Ferraz de Menezes, Catherine Malinda Harvey, Marleny Elizabeth Márquez de Martínez Gerbi, Zachary J. Smith, Dan Smith, Juan C. Ivaldi, Alton Phillips, James W. Chan and Sebastian Wachsmann-Hogiu

      Version of Record online: 24 MAY 2017 | DOI: 10.1002/jbio.201700042

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      Thermal effects of femtosecond lasers for the removal of carious dental tissue have been investigated. Minimal morphological damages of teeth are incurred at repetition rates below the carbonization threshold, while minimal thermal damage (<5 °C temperature increase) occurs at repetition rates at or below 10kHz with a laser fluence of 40 J/cm2. fsLIBS spectra were recorded to evaluate the differences between normal and diseased teeth as well as between different tooth components.

    6. Reflectance spectroscopy: to shed new light on the capillary refill test

      Rani Toll John, Joakim Henricson, Gert E Nilsson, Daniel Wilhelms and Chris D. Anderson

      Version of Record online: 24 MAY 2017 | DOI: 10.1002/jbio.201700043

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      Polarized reflectance spectroscopy enables quantitative assessment of the return of blood to the blanched area, during the Capillary Refill (CR) test. CR test is currently performed at various body sites normally by naked eye assessment. The reliability and validity of the CR test has been questioned. Quantitative CR responses show site and temperature variability. The availability of objective endpoint values would increase the utility of the CR test.

    7. FSOCA-induced switchable footpad skin optical clearing window for blood flow and cell imaging in vivo

      Rui Shi, Wei Feng, Chao Zhang, Zhihong Zhang and Dan Zhu

      Version of Record online: 18 MAY 2017 | DOI: 10.1002/jbio.201700052

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      An innovative FSOCA was developed to make the footpad skin transparent, which provided a switchable window for blood flow and cell imaging in vivo. The results showed that the cutaneous blood flow can be monitored with higher contrast, and fluorescent cells can be imaged with higher signal intensity and larger depth after FSOCA treatment. This switchable footpad skin optical clearing window will be significant for microcirculation, immunology, and other diseases studies.

    8. You have free access to this content
      Intrinsic fluorescence and mechanical testing of articular cartilage in human patients with osteoarthritis

      Juan Pablo Padilla-Martinez, William Lewis, Antonio Ortega-Martinez and Walfre Franco

      Version of Record online: 18 MAY 2017 | DOI: 10.1002/jbio.201600269

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      Fluorescence spectroscopy and mechanical testing were performed on articular cartilage samples taken from six patients with osteoarthritis undergoing joint surgery. After stratification of cartilage based on degree of damage, an association was observed between cartilage stiffness and fluorescence at 330 nm excitation/390 nm emission (R=0.82, p=0.04). The fluorescence of physiologic crosslinks and advanced glycation end-products may offer utility for the characterization of cartilage in OA.

    9. Evaluation of detection distance-dependent reflectance spectroscopy for the determination of the sun protection factor using pig ear skin

      Carina Reble, Ingo Gersonde, Sabine Schanzer, Martina C. Meinke, Jürgen Helfmann and Jürgen Lademann

      Version of Record online: 18 MAY 2017 | DOI: 10.1002/jbio.201600257

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      Spatially resolved reflectance spectroscopy is applied for the determination of the sun protection factor with a UV dose below the minimal erythema limit. Experiments on pig ear skin using 5 different sunscreens are presented and compared to reference values from certified test institutes.

    10. Two-photon imaging of fiber-coupled neurons

      M.S. Pochechuev, I.V. Fedotov, O.I. Ivashkina, M.A. Roshchina, K.V. Anokhin and A.M. Zheltikov

      Version of Record online: 18 MAY 2017 | DOI: 10.1002/jbio.201600203

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      Fiber-optic components offer a versatile platform for the development of innovative neurointerfaces, as well as flexible microprobes for deep-brain optical interrogation. The development of methods whereby optical fibers could be reproducibly coupled to individual neurons stands alone as a fundamental challenge. Here, we apply two-photon imaging as a tool to validate optical coupling between a fiber-optic probe and individual neurons in a live brain of transgenic mice.

    11. Near–infrared photodynamic inactivation of S. pneumoniae and its interaction with RAW 264.7 macrophages

      Ilaiáli S. Leite, Mariana C. Geralde, Ana C.G. Salina, Alexandra I. Medeiros, Lívia N. Dovigo, Vanderlei S. Bagnato and Natalia M. Inada

      Version of Record online: 18 MAY 2017 | DOI: 10.1002/jbio.201600283

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      Indocyanine green and two near-infrared light sources (left: 780 nm lasers; right: 850 nm LEDs) are used to efficiently inactivate Streptococcus pneumoniae and have low impact on RAW 264.7 macrophages. Bacteria-macrophage co-cultures experiments indicate a positive interaction of indocyanine green-mediated photodynamic therapy with macrophage's killing action.

    12. Optical coherence elastography for strain dynamics measurements in laser correction of cornea shape

      Vladimir Y. Zaitsev, Alexander L. Matveyev, Lev A. Matveev, Grigory V. Gelikonov, Alexander I. Omelchenko, Olga I. Baum, Sergey E. Avetisov, Andrey V. Bolshunov, Vladimir I. Siplivy, Dmitry V. Shabanov, Alex Vitkin and Emil N. Sobol

      Version of Record online: 11 MAY 2017 | DOI: 10.1002/jbio.201600291

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      OCT images of an excised rabbit eye cornea before and after application of a non-destructive laser-reshaping procedure using an IR laser. The applied 50-second pulse-periodic laser-induced moderate heating (∼45–55 °C) made it possible to noticeably reshape cornea (e. g., produce/smoothen indentations) without causing tissue coagulation and worsening its optical properties. The inset shows examples of reconstructed dynamic (inter-frame) and cumulative strains obtained during thermo-mechanical smoothing of the indentation shown in the upper panels.