Journal of Biophotonics

Cover image for Vol. 10 Issue 5

Early View (Online Version of Record published before inclusion in an issue)

Editor: Jürgen Popp

Impact Factor: 3.818

ISI Journal Citation Reports © Ranking: 2015: 10/90 (Optics); 16/77 (BIOCHEMICAL RESEARCH METHODS); 16/72 (Biophysics)

Online ISSN: 1864-0648

Associated Title(s): Laser & Photonics Reviews

  1. Full Articles

    1. 3D in vivo imaging with extended-focus optical coherence microscopy

      Yu Chen, Le A. Trinh, Jeff Fingler and Scott E. Fraser

      Version of Record online: 18 APR 2017 | DOI: 10.1002/jbio.201700008

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      This paper presents an xf-OCM imaging system for 3D biological imaging with 1.6-mm lateral resolution and 2.9-mm axial resolution, and DOF of ∼100 mm that was extended by ∼3X than standard OCM. It combines high spatial resolution, large penetration depth and insensitivity to spherical abberrations, which is well suited to label-freee imaging of living biological samples.

    2. Quantitative cognitive-test characterization of reconnectable implantable fiber-optic neurointerfaces for optogenetic neurostimulation

      I. V. Fedotov, O. I. Ivashkina, M. S. Pochechuev, M. A. Roshchina, K. A. Toropova, A. B. Fedotov, K. V. Anokhin and A. M. Zheltikov

      Version of Record online: 23 FEB 2017 | DOI: 10.1002/jbio.201600225

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      With the optical methods of brain research advancing toward challenging goals of understanding the cell biology behind learning and memory, accurate quantitative characterization of optogenetic neurostimulation in live brain and its cognitive effects becomes even more important. Here, we show that cognitive tests on representative groups of freely behaving transgenic mice enable such a quantitative characterization of optogenetic neurostimulation, allowing the performance of reconnectable optical fibers to be accurately quantified.

    3. Development and application of LED arrays for use in phototherapy research

      Mohammed A. Hadis, Paul R. Cooper, Michael R. Milward, Patricia. C. Gorecki, Edward Tarte, James Churm and William M. Palin

      Version of Record online: 6 FEB 2017 | DOI: 10.1002/jbio.201600273

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      LED micro arrays and a rapid assay method that can be used to identify irradiation parameters in low level light therapy (LLLT), photodynamic therapy and photodissinfection are developed and its application is demonstrated in vitro using dental pulp cells. The method provides a robust and reliable platform for hight-hroughput analysis of biological effects which has time-saving and is cost-effective for use in research laboratories.

    4. Holographic optical tweezers-based in vivo manipulations in zebrafish embryos

      Florian Hörner, Robert Meissner, Sruthi Polali, Jana Pfeiffer, Timo Betz, Cornelia Denz and Erez Raz

      Version of Record online: 6 FEB 2017 | DOI: 10.1002/jbio.201600226

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      We established a procedure allowing the use of holographic optical tweezers for the purpose of manipulating cellular organelles and moving particles introduced into cells. The method allows the characterization of mechanical properties distribution within cells, in the physiological environment of the developing early vertebrate embryo. The method is demonstrated by manipulating the shape of the cell nucleus, and by comparing mechanical features at different locations within the cell, using the delay in bead oscillation as readout.

    5. Real-time Raman and SRS imaging of living human macrophages reveals cell-to-cell heterogeneity and dynamics of lipid uptake

      Clara Stiebing, Tobias Meyer, Ingo Rimke, Christian Matthäus, Michael Schmitt, Stefan Lorkowski and Jürgen Popp

      Version of Record online: 6 FEB 2017 | DOI: 10.1002/jbio.201600279

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      The unregulated uptake of lipids by macrophages is a crucial factor for the initiation of atherosclerotic plaque in arterial walls. Based on spontaneous and stimulated Raman imaging, a complete overview of the uptake behavior of deuterated palmitic acid by living macrophages is presented. Striking cell-to-cell heterogeneity in the uptake kinetics was observed for the first time.

    6. Low-level light emitting diode (LED) therapy suppresses inflammasome-mediated brain damage in experimental ischemic stroke

      Hae In Lee, Sae-Won Lee, Nam Gyun Kim, Kyoung-Jun Park, Byung Tae Choi, Yong-Il Shin and Hwa Kyoung Shin

      Version of Record online: 6 FEB 2017 | DOI: 10.1002/jbio.201600244

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      This study identified a novel therapeutic mechanism in cases of ischemic damage that relies on suppressing neuroinflammation after the insult itself. Post-ischemic low-level light emitting diode (LED) therapy (LED-T; 610 nm, 2 J/cm2) suppressed neuroinflammation and neural cell death in the ischemic cortex via TLR2-mediated activation and the NLRP3 inflammasome, and that this activation was in turn mediated through MAPK and NF-kB pathways. Notably, neurological scores were improved after LED therapy. Our findings suggest that therapeutic interventions targeting the inflammasome with LED would be beneficial to ameliorate brain injury following ischemic stroke.

    7. Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging

      Y. X. Li, V. Gautam, A. Brüstle, I. A. Cockburn, V. R. Daria, C. Gillespie, K. Gaus, C. Alt and W. M. Lee

      Version of Record online: 6 FEB 2017 | DOI: 10.1002/jbio.201600289

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      Modern multiphoton microscopy demands the smooth integration of software and hardware so as to conduct cutting edge in-vivo imaging at single cell level. We introduced an advanced control module capable of freely controlling scanning speed of polygon-mirror and imaging formats on-the-fly. The high dynamic scanning (ms to µs) was used to capture neuron transients and blood flow (mm/s) at single cell level over a large field of view (>500 µm).

    8. Differential standard deviation of log-scale intensity based optical coherence tomography angiography

      Weisong Shi, Wanrong Gao, Chaoliang Chen and Victor X. D. Yang

      Version of Record online: 30 JAN 2017 | DOI: 10.1002/jbio.201600264

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      Differential standard deviation of log-scale intensity (DSDLI) based optical coherence tomography angiography (OCTA) was presented to enhance the contrast of microvasculature maps. The signal noise ratio and the spatial resolution of blood vessels are both improved greatly with our method compared to speckle variance OCT and power intensity method, which suggests a promising application of DSDLI in the disease diagnosis and monitoring.

    9. A comparative study of ex vivo skin optical clearing using two-photon microscopy

      Anton Sdobnov, Maxim E. Darvin, Juergen Lademann and Valery Tuchin

      Version of Record online: 30 JAN 2017 | DOI: 10.1002/jbio.201600066

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      The aim of this study was to demonstrate the abilities of glycerol and OmnipaqueTM solutions to improve the optical clearing of porcine skin to provide diagnostics and monitoring of skin diseases by Multi-Photon Tomography. Based on received quantitative data, it was concluded that OmnipaqueTM is an effective and promising agent for optical clearing as it has a much lower or no measureable impact on tissue morphology in comparison with glycerol.

    10. Triglyceride dependent differentiation of obesity in adipose tissues by FTIR spectroscopy coupled with chemometrics

      Fatma Kucuk Baloglu, Onur Baloglu, Sebastian Heise, Gudrun Brockmann and Feride Severcan

      Version of Record online: 27 JAN 2017 | DOI: 10.1002/jbio.201600223

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      In the current study, it was proposed that triglyceride band can be used as a specific spectral biomarker for the diagnosis of obesity. Fourier Transform Infrared (FTIR) Spectroscopy coupled with unsupervised chemometric methods was used to determine obesity-dependent molecular alterations of adipose tissue in the spectral region of triglyceride band. Correspondingly, the control, obese and obesity related insulin resistant groups were distinguished successfully in visceral and subcutaneous adipose tissues.

    11. The effects of low level laser therapy on both HIV-1 infected and uninfected TZM-bl cells

      Masixole Yvonne Lugongolo, Sello Lebohang Manoto, Saturnin Ombinda-Lemboumba, Malik Maaza and Patience Mthunzi-Kufa

      Version of Record online: 27 JAN 2017 | DOI: 10.1002/jbio.201600217

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      In a dose dependent manner, laser irradiation had detrimental effects on HIV-1 infected cells as seen by an increase in cell rounding (B), and increased levels of LDH in medium (B). However, irradiation in the absence of infection had no inhibitory influence on uninfected cells. Laser irradiation reduced HIV-1 expression in irradiated cells (C).

    12. Inverted multiscale optical resolution photoacoustic microscopy

      Weizhi Qi, Tian Jin, Jian Rong, Huabei Jiang and Lei Xi

      Version of Record online: 27 JAN 2017 | DOI: 10.1002/jbio.201600246

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      An inverted optical resolution photoacoustic microscopy system with multiscale FOVs and resolutions is developed. A cylindrically focused transducer and a two-dimensional galvanometric scanner are integrated to achieve a hybrid optical-mechanical scanning mechanism. Black tapes, carbon fibers, and sharp blades are imaged to evaluate the performance of the system, and in vivo multiscale imaging of vasculatures inside the ears and brains of mice is demonstrated using this system.

    13. Atherectomy using a solid-state laser at 355 nm wavelength

      Amir Herzog, Grzegorz Oszkinis, David Planer, Krzysztof Ziaja, Łukasz Kruszyna, Michał Goran Stanisić, Damian Ziaja, Amiel A. Ishaaya and Wacław Kuczmik

      Version of Record online: 20 JAN 2017 | DOI: 10.1002/jbio.201600209

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      Peripheral arterial disease (PAD) is allied with an increased risk of ischemic events and death. In this work, pulses of a solid-state laser at 355 nm delivered through a hybrid catheter are used to conduct a pre-clinical study and the first two clinical cases. Successful recanalization of calcified totally occluded arteries is obtained and constitutes a proof of concept for using a solid-state pulsed laser at 355 nm in atherectomy.

    14. Confocal laser endomicroscopy in head and neck malignancies using FITC-labelled EpCAM- and EGF-R-antibodies in cell lines and tumor biopsies

      Anna S. Englhard, Alexander Palaras, Veronika Volgger, Herbert Stepp, Brigitte Mack, Darko Libl, Olivier Gires and Christian S. Betz

      Version of Record online: 20 JAN 2017 | DOI: 10.1002/jbio.201600238

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      Optimizing detection of resection margins during tumor surgery is a promising means to reduce local recurrence. Here, the use of antibodies specific for the tumor antigens EGF-R and EpCAM as probes for confocal laser scanning endomicroscopy (CLE) was evaluated in vitro on permanent cell lines and ex vivo on HNSCC specimens and healthy mucosa. Both, EpCAM and EGF-R are promising candidates for CLE-mediated detection of residual carcinoma cells.

    15. Cypate-mediated thermosensitive nanoliposome for tumor imaging and photothermal triggered drug release

      Zhihao Han, Liwei Lv, Yi Ma, Zhaohui Wang, Yuxi Liu, Min Zhang, Siwen Li and Yueqing Gu

      Version of Record online: 20 JAN 2017 | DOI: 10.1002/jbio.201600270

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      Nanoscale drug delivery systems are attracting considerable attention as an alternative strategy to overcome a lot of limitation. The multifunctional photothermal sensitive liposomes drug-loading system was established, with cypate and DOX carried in it for multi-mechanism diagnosis and treatment of tumor diseases. The attractive features of DOX@PTSL demonstrated here support the anticipation of its promising prospect in cancer therapy.

    16. Localized measurements of physical parameters within human sperm cells obtained with wide-field interferometry

      Michal Balberg, Mattan Levi, Ksawery Kalinowski, Itay Barnea, Simcha K. Mirsky and Natan T. Shaked

      Version of Record online: 12 JAN 2017 | DOI: 10.1002/jbio.201600186

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      Quantitative measurements of the dry mass of human spermatozoa, their nuclei (black contour), and acrosomes (white contour), are obtained using interference phase microscopy (IPM). In addition, the refractive index and the thickness of dried sperm heads and their organelles are obtained. Our findings enable one to evaluate the distribution of these quantifiable physical parameters in order to extract morphological information from label-free spermatozoa for the benefit of in-vitro fertilization.

    17. Identification of morphological and biochemical changes in keratin-8/18 knock-down cells using Raman spectroscopy

      S. P. Singh, Hunain Alam, Crismita Dmello, Hitesh Mamgain, Milind M. Vaidya, Ramachandra Rao Dasari and C. Murali Krishna

      Version of Record online: 9 JAN 2017 | DOI: 10.1002/jbio.201600249

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      In the present study, Raman spectroscopy, both fiber-optic and microscopy, were explored to study changes due to loss of ‘keratin' protein in tongue cancer cells. A microscopic approach was explored to study changes at the single-cell level and applicability of less expensive and commercially available for fiber-optic probes on cell pellets was also assessed. The findings can help in designing novel keratin-based ‘theranostics' tools for oral cancers.

    18. Multimodal fiber-probe spectroscopy allows detecting epileptogenic focal cortical dysplasia in children

      Suresh Anand, Riccardo Cicchi, Flavio Giordano, Valerio Conti, Anna Maria Buccoliero, Renzo Guerrini and Francesco Saverio Pavone

      Version of Record online: 9 JAN 2017 | DOI: 10.1002/jbio.201600136

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      Here we investigate the application of fluorescence spectroscopy (at 378 nm and 445 nm excitation) and Raman spectroscopy (at 785 nm excitation) to discriminate normal brain tissue and focal cortical dysplasia. Multimodal spectroscopic approach yielded 100% sensitivity and 90% specificity by exploiting the information obtained from different spectroscopic techniques. Our results demonstrate the application of multimodal spectroscopic approach for the possible surgical resection of the dysplastic tissue during surgery for epilepsy.

    19. Phagocytosis and immune response studies of Macrophage-Nanodiamond Interactions in vitro and in vivo

      K.-J. Huang, C.-Y. Lee, Y.-C. Lin, C.-Y. Lin, E. Perevedentseva, S.-F. Hung and C.-L. Cheng

      Version of Record online: 9 JAN 2017 | DOI: 10.1002/jbio.201600202

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      The applications of nanodiamond (ND) as drug delivery and bio-imaging can require the relinquishing ND-drug conjugate via blood flow, where interaction with immune cells may occur. In this work, we investigated the ND penetration in macrophage and the immune response using the tissue-resident murine macrophages (RAW 264.7). It was shown that ND didn't stimulate the immune response, and functionality of the macrophage was not altered. Using MTT test, ND was found negligibly cytotoxic to macrophages. Thus, ND can serve as a biocompatible platform for bio-medical applications.

    20. Comparative study of presurgical skin infiltration depth measurements of melanocytic lesions with OCT and high frequency ultrasound

      Arthur Varkentin, Mikhail Mazurenka, Elias Blumenröther, Merve Meinhardt-Wollweber, Maik Rahlves, Sigrid M. C. Broekaert, Susanne Schäd-Trcka, Steffen Emmertinst, Uwe Morgner and Bernhard Roth

      Version of Record online: 23 DEC 2016 | DOI: 10.1002/jbio.201600139

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      Malignant melanoma is the most dangerous skin cancer type. A very important criteria for risk evaluation is the skin infiltration depth of the melanoma. Non-invasive preoperative in vivo thickness assessment can provide additional information for histopathologists and has the potential to decrease false positive cases and avoid different steps of surgical intervention. With optical coherence tomography as well as high frequency ultrasound such thickness assessment is possible.