Journal of Biophotonics

Cover image for Vol. 10 Issue 10

Early View (Online Version of Record published before inclusion in an issue)

Editor: Jürgen Popp

Impact Factor: 4.328

ISI Journal Citation Reports © Ranking: 2016: 10/92 (Optics); 12/77 (BIOCHEMICAL RESEARCH METHODS); 15/72 (Biophysics)

Online ISSN: 1864-0648

Associated Title(s): Laser & Photonics Reviews


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  1. Full Articles

    1. Optofluidic organization and transport of cell chain

      Xiaoshuai Liu, Jianbin Huang, Yuchao Li, Yao Zhang and Baojun Li

      Version of Record online: 2 MAY 2017 | DOI: 10.1002/jbio.201600306

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      Non-contact optofluidic organization of cell chain were demonstrated by implanting a large-tapered-angle fiber probe (LTAP) into the microfluidic platform, and the organized cell chain can be transported via the adjustment of laser power and flow velocity.

    2. Measurement of optical properties of pig esophagus by using a modified spectrometer set-up

      Martin Hohmann, B. Lengenfelder, R. Kanawade, F. Klämpfl, A. Douplik and H. Albrecht

      Version of Record online: 2 MAY 2017 | DOI: 10.1002/jbio.201600187

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      Optical properties (μa, μs and g) of certain human tissue types such as skin and blood have been very well investigated. However until today, for internal body organs such as the esophagus they are not well characterized. For ex-vivo measurements “Inverse Adding Doubling” (IAD) and Inverse Monte-Carlo-Simulation (IMCS) are state of the art. Both methods need the measurement of the collimated transmission. For this measurement of the g-factor a new approach has been developed and evaluated with intralipid.

    3. Low–level light emitting diode therapy promotes long–term functional recovery after experimental stroke in mice

      Hae In Lee, Sae-Won Lee, Nam Gyun Kim, Kyoung-Jun Park, Byung Tae Choi, Yong-Il Shin and Hwa Kyoung Shin

      Version of Record online: 2 MAY 2017 | DOI: 10.1002/jbio.201700038

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      This study aimed to investigate the effects of low-level light emitting diode therapy (LED-T) on the long-term functional outcomes after cerebral ischemia, and the optimal timing of LED-T initiation for achieving suitable functional recovery. we clearly found that LED-T administered during the subacute stage had a positive impact on the long-term functional outcome, probably via neuron and astrocyte proliferation, blood vessel reconstruction, and increased brain-derived neurotrophic factor expression.

    4. Light-driven photosensitizer uptake increases Candida albicans photodynamic inactivation

      Renan A. Romano, Sebastião Pratavieira, Ana P. da Silva, Cristina Kurachi and Francisco E. G. Guimarães

      Version of Record online: 2 MAY 2017 | DOI: 10.1002/jbio.201600309

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      Photosensitizer uptake can be increased by a light-driven incubation in C. albicans cells, leading to a lower survival rate comparing with the traditional dark incubation protocol.

    5. Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies

      Zachary A. Steelman, Will J. Eldridge, Jacob B. Weintraub and Adam Wax

      Version of Record online: 18 APR 2017 | DOI: 10.1002/jbio.201600314

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      Recent studies using quantitative phase microscopy have suggested that the nuclear refractive index is lower than that of cytoplasm. In this paper, we verify this claim in new cell lines, including non-cancerous and primary lines. We further validate against potential corrupting factors, and examine the impact that a low nuclear index would have on light scattering technologies.

    6. Treatment of tumor in lymph nodes using near-infrared laser light-activated thermosensitive liposome-encapsulated doxorubicin and gold nanorods

      Daisuke Matsuki, Oladipo Adewale, Sachiko Horie, Junnosuke Okajima, Atsuki Komiya, Oluwatobi Oluwafemi, Shigenao Maruyama, Shiro Mori and Tetsuya Kodama

      Version of Record online: 18 APR 2017 | DOI: 10.1002/jbio.201600241

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      Tumor metastasis to lymph nodes is an important contributory factor for cancer-related deaths despite recent developments in cancer therapy. This study demonstrates that tumor in the lymph node of the mouse can be treated by the external near-infrared laser light to trigger the unloading of doxorubicin encapsulated in thermosensitive liposomes administered together with gold nanorods. This work presents a theranostic approach that has the potential to treat metastatic lymph nodes.

    7. IR-spectroscopy of skin in vivo: Optimal skin sites and properties for non-invasive glucose measurement by photoacoustic and photothermal spectroscopy

      Alexander Bauer, Otto Hertzberg, Arne Küderle, Dominik Strobel, Miguel A. Pleitez and Werner Mäntele

      Version of Record online: 18 APR 2017 | DOI: 10.1002/jbio.201600261

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      Four different locations on the human arm and hand are investigated for their suitability for non-invasive glucose monitoring with photoacoustic and photothermal detection. In vivo mid-IR spectra of skin are recorded and correlated with invasively measured blood glucose employing partial least squares regression. Furthermore, both techniques are used to study the effects of the reconstitution of the skin's acid mantle after washing.

    8. Light-Emitting Diode treatment ameliorates allergic lung inflammation in experimental model of asthma induced by ovalbumin

      Vanessa Perosino Cardoso Siqueira, Marina Izadora Silveira Evangelista, Alana dos Santos, Rodrigo Labat Marcos, Ana Paula Ligeiro-de-Oliveira, Christiane Pavani, Amílcar Sabino Damazo and Adriana Lino-dos-Santos-Franco

      Version of Record online: 18 APR 2017 | DOI: 10.1002/jbio.201600247

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      Asthma is a multifactorial disease where treatment sometimes is not effective and, in this context new therapies are of great importance. Phototherapy as Light-emitting diode (LED) has emerged as a promisor treatment presenting good results for diseases which are characterized by inflammation. This study open the possibility of a new approach for the asthma treatment showing that drug therapy chould be replaced by LED treatment.

    9. Enhanced Phototoxicity of Photodynamic Treatment by Cx26-composed GJIC via ROS-, calcium- and lipid peroxide-mediated pathways

      Deng-Pan Wu, Tian-Yu Lin, Li-Ru Bai, Jin-Lan Huang, Yan Zhou, Nan Zhou, Sheng-Lei Zhong, Shan Gao and Xiao-Xing Yin

      Version of Record online: 18 APR 2017 | DOI: 10.1002/jbio.201600255

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      In spite of promising initial treatment responses after PDT, recurrences remain at high level. Consequently, there is need for improvement in the effectiveness of PDT. Our study showed that Cx26-composed GJIC increased PDT phototoxicity in transfected HeLa cells, which was related with ROS, calcium and lipid peroxide-mediated pathways. Our study indicates the possibility that maintenance or increase of Cx26-formed GJIC may be used to increase the efficacy of PDT.

    10. 3D in vivo imaging with extended-focus optical coherence microscopy

      Yu Chen, Le A. Trinh, Jeff Fingler and Scott E. Fraser

      Version of Record online: 18 APR 2017 | DOI: 10.1002/jbio.201700008

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      This paper presents an xf-OCM imaging system for 3D biological imaging with 1.6-mm lateral resolution and 2.9-mm axial resolution, and DOF of ∼100 mm that was extended by ∼3X than standard OCM. It combines high spatial resolution, large penetration depth and insensitivity to spherical abberrations, which is well suited to label-freee imaging of living biological samples.

    11. Quantitative cognitive-test characterization of reconnectable implantable fiber-optic neurointerfaces for optogenetic neurostimulation

      I. V. Fedotov, O. I. Ivashkina, M. S. Pochechuev, M. A. Roshchina, K. A. Toropova, A. B. Fedotov, K. V. Anokhin and A. M. Zheltikov

      Version of Record online: 23 FEB 2017 | DOI: 10.1002/jbio.201600225

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      With the optical methods of brain research advancing toward challenging goals of understanding the cell biology behind learning and memory, accurate quantitative characterization of optogenetic neurostimulation in live brain and its cognitive effects becomes even more important. Here, we show that cognitive tests on representative groups of freely behaving transgenic mice enable such a quantitative characterization of optogenetic neurostimulation, allowing the performance of reconnectable optical fibers to be accurately quantified.

    12. Development and application of LED arrays for use in phototherapy research

      Mohammed A. Hadis, Paul R. Cooper, Michael R. Milward, Patricia. C. Gorecki, Edward Tarte, James Churm and William M. Palin

      Version of Record online: 6 FEB 2017 | DOI: 10.1002/jbio.201600273

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      LED micro arrays and a rapid assay method that can be used to identify irradiation parameters in low level light therapy (LLLT), photodynamic therapy and photodissinfection are developed and its application is demonstrated in vitro using dental pulp cells. The method provides a robust and reliable platform for hight-hroughput analysis of biological effects which has time-saving and is cost-effective for use in research laboratories.

    13. Holographic optical tweezers-based in vivo manipulations in zebrafish embryos

      Florian Hörner, Robert Meissner, Sruthi Polali, Jana Pfeiffer, Timo Betz, Cornelia Denz and Erez Raz

      Version of Record online: 6 FEB 2017 | DOI: 10.1002/jbio.201600226

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      We established a procedure allowing the use of holographic optical tweezers for the purpose of manipulating cellular organelles and moving particles introduced into cells. The method allows the characterization of mechanical properties distribution within cells, in the physiological environment of the developing early vertebrate embryo. The method is demonstrated by manipulating the shape of the cell nucleus, and by comparing mechanical features at different locations within the cell, using the delay in bead oscillation as readout.

    14. Low-level light emitting diode (LED) therapy suppresses inflammasome-mediated brain damage in experimental ischemic stroke

      Hae In Lee, Sae-Won Lee, Nam Gyun Kim, Kyoung-Jun Park, Byung Tae Choi, Yong-Il Shin and Hwa Kyoung Shin

      Version of Record online: 6 FEB 2017 | DOI: 10.1002/jbio.201600244

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      This study identified a novel therapeutic mechanism in cases of ischemic damage that relies on suppressing neuroinflammation after the insult itself. Post-ischemic low-level light emitting diode (LED) therapy (LED-T; 610 nm, 2 J/cm2) suppressed neuroinflammation and neural cell death in the ischemic cortex via TLR2-mediated activation and the NLRP3 inflammasome, and that this activation was in turn mediated through MAPK and NF-kB pathways. Notably, neurological scores were improved after LED therapy. Our findings suggest that therapeutic interventions targeting the inflammasome with LED would be beneficial to ameliorate brain injury following ischemic stroke.

    15. Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging

      Y. X. Li, V. Gautam, A. Brüstle, I. A. Cockburn, V. R. Daria, C. Gillespie, K. Gaus, C. Alt and W. M. Lee

      Version of Record online: 6 FEB 2017 | DOI: 10.1002/jbio.201600289

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      Modern multiphoton microscopy demands the smooth integration of software and hardware so as to conduct cutting edge in-vivo imaging at single cell level. We introduced an advanced control module capable of freely controlling scanning speed of polygon-mirror and imaging formats on-the-fly. The high dynamic scanning (ms to µs) was used to capture neuron transients and blood flow (mm/s) at single cell level over a large field of view (>500 µm).

    16. Differential standard deviation of log-scale intensity based optical coherence tomography angiography

      Weisong Shi, Wanrong Gao, Chaoliang Chen and Victor X. D. Yang

      Version of Record online: 30 JAN 2017 | DOI: 10.1002/jbio.201600264

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      Differential standard deviation of log-scale intensity (DSDLI) based optical coherence tomography angiography (OCTA) was presented to enhance the contrast of microvasculature maps. The signal noise ratio and the spatial resolution of blood vessels are both improved greatly with our method compared to speckle variance OCT and power intensity method, which suggests a promising application of DSDLI in the disease diagnosis and monitoring.

    17. Inverted multiscale optical resolution photoacoustic microscopy

      Weizhi Qi, Tian Jin, Jian Rong, Huabei Jiang and Lei Xi

      Version of Record online: 27 JAN 2017 | DOI: 10.1002/jbio.201600246

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      An inverted optical resolution photoacoustic microscopy system with multiscale FOVs and resolutions is developed. A cylindrically focused transducer and a two-dimensional galvanometric scanner are integrated to achieve a hybrid optical-mechanical scanning mechanism. Black tapes, carbon fibers, and sharp blades are imaged to evaluate the performance of the system, and in vivo multiscale imaging of vasculatures inside the ears and brains of mice is demonstrated using this system.

    18. Cypate-mediated thermosensitive nanoliposome for tumor imaging and photothermal triggered drug release

      Zhihao Han, Liwei Lv, Yi Ma, Zhaohui Wang, Yuxi Liu, Min Zhang, Siwen Li and Yueqing Gu

      Version of Record online: 20 JAN 2017 | DOI: 10.1002/jbio.201600270

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      Nanoscale drug delivery systems are attracting considerable attention as an alternative strategy to overcome a lot of limitation. The multifunctional photothermal sensitive liposomes drug-loading system was established, with cypate and DOX carried in it for multi-mechanism diagnosis and treatment of tumor diseases. The attractive features of DOX@PTSL demonstrated here support the anticipation of its promising prospect in cancer therapy.

    19. Spectral diffuse reflectance and autofluorescence imaging can perform early prediction of blood vessel occlusion in skin flaps

      Shuo Chen, Caigang Zhu, Christopher Hoe-Kong Chui, Gyanendra Sheoran, Bien-Keem Tan and Quan Liu

      Version of Record online: 17 NOV 2016 | DOI: 10.1002/jbio.201600189

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      In this study, spectral diffuse reflectance and autofluorescence imaging data and reconstructed spectra were used to statistically differentiate control flaps, arterially occluded flaps and venously occluded flaps in a rat skin flap model. Our preliminary results suggest that the proposed spectroscopic imaging technique can achieve high classification accuracy thus could detect and differentiate flaps with venous and arterial occlusion accurately at an early time point in a large skin flap.

    20. Tail artifact removal in OCT angiography images of rodent cortex

      Utku Baran, Woo June Choi, Yuandong Li and Ruikang K. Wang

      Version of Record online: 7 SEP 2016 | DOI: 10.1002/jbio.201600194

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      This paper proposes a simple image processing method to remove the tail artifacts in depth-resolved OCT angiography images. Here, OCT structural images are utilized to generate an adaptive enface mask for removal of these artifacts. The effectiveness of this method is shown by comparing vessel densities and vessel similarities of depth-resolved OCT angiograms in deeper layers of healthy and ischemic rodent cortex, in vivo.


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