Journal of Biophotonics

Cover image for Vol. 10 Issue 1

Early View (Online Version of Record published before inclusion in an issue)

Editor: Jürgen Popp

Impact Factor: 3.818

ISI Journal Citation Reports © Ranking: 2015: 10/90 (Optics); 16/77 (BIOCHEMICAL RESEARCH METHODS); 16/72 (Biophysics)

Online ISSN: 1864-0648

Associated Title(s): Laser & Photonics Reviews


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  1. Full Articles

    1. Multiparametric evaluation of hindlimb ischemia using time-series indocyanine green fluorescence imaging

      Huizhi Guang, Chuangjian Cai, Simin Zuo, Wenjuan Cai, Jiulou Zhang and Jianwen Luo

      Version of Record online: 2 MAY 2016 | DOI: 10.1002/jbio.201600029

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      Perfusion rate, vascular perfusion density and time-varying ICG concentration in veins are estimated simultaneously to quantitatively evaluate and compare the vascular perfusion of the normal and ischemic hindlimbs using time-series ICG imaging, which can monitor blood perfusion in vivo. These parameters may provide more effective information for diagnosis of vascular occlusive disease.

    2. Feasibility of photoacoustic evaluations on dual-thermal treatment of ex vivo bladder tumors

      Van Phuc Nguyen, Junghwan Oh, Suhyun Park and Hyun Wook Kang

      Version of Record online: 2 MAY 2016 | DOI: 10.1002/jbio.201600045

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      This paper introduces the feasible application of photoacoustic imaging (PAI) for laser-integrated high intensity focused ultrasound (HIFU) treatment on ex vivo bladder tissue injected with artificial tumor. Due to additive thermal effects at low power levels, the proposed thermal treatment coagulates the targeted tissue. PAI ostensibly identifies the locations of both the artificial and the coagulated tumors with high image contrast. The PAI-guided dual thermal treatments can be a feasible therapeutic modality to treat bladder tumors in an effective manner.

    3. Colocalization of cellular nanostructure using confocal fluorescence and partial wave spectroscopy

      John E. Chandler, Yolanda Stypula-Cyrus, Luay Almassalha, Greta Bauer, Leah Bowen, Hariharan Subramanian, Igal Szleifer and Vadim Backman

      Version of Record online: 25 APR 2016 | DOI: 10.1002/jbio.201500298

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      Instrumentation has been developed enabling colocalization of confocal fluorescence and nanoscale-sensitive spectroscopic microscopy modalities. This combination allows sensing of the heterogeneity of nanoscale intracellular structure within specific fluorescently labeled organelles or molecular structures. To demonstrate the capabilities of this multimodal instrument, the effects on the heterogeneity of nanoscale structure were examined in the nuclei and mitochondria of HeLa cells treated with valinomycin, a potassium ionophore that uncouples oxidative phosphorylation.

    4. Minimally invasive screening for colitis using attenuated total internal reflectance fourier transform infrared spectroscopy

      Jitto Titus, Emilie Viennois, Didier Merlin and A. G. Unil Perera

      Version of Record online: 20 APR 2016 | DOI: 10.1002/jbio.201600041

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      This paper describes a minimally invasive, cost effective spectroscopic technique as a potential screening test for colitis. This simple low risk alternative to biological assays and colonoscopies could lead to a futuristic portable point of care tool similar to a glucose testing meter thereby increasing the possibility of identifying colitis and other gastrointestinal disorders at an early stage.

    5. A method for monitoring of oxygen saturation changes in brain tissue using diffuse reflectance spectroscopy

      Peter Rejmstad, Johannes D. Johansson, Neda Haj-Hosseini and Karin Wårdell

      Version of Record online: 20 APR 2016 | DOI: 10.1002/jbio.201500334

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      Diffuse reflectance spectroscopy is used together with an analytical method for real-time estimation of local oxygen saturation. The algorithm is developed from a set of synthetic spectra generated with the P3-approximation using hemoglobin absorption and scattering mimicking human white matter together with experimental phantom measurements and oxygen pressure regulation for different blood concentrations. The method is exemplified on clinical data collected during brain surgery.

    6. Complex refractive index of normal and malignant human colorectal tissue in the visible and near-infrared

      Panagiotis Giannios, Spyridon Koutsoumpos, Konstantinos G. Toutouzas, Maria Matiatou, George C. Zografos and Konstantinos Moutzouris

      Version of Record online: 19 APR 2016 | DOI: 10.1002/jbio.201600001

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      Multi-wavelength prism coupling refractometry is employed for the ex-vivo reflectance measurement of freshly excised human colorectal specimens. Novel data of the complex refractive index of human intestinal tissue are provided and the index contrast between colorectal tumors and healthy mucosa is demonstrated as a marker of disease, suggesting its potential for novel diagnostic tools in endoscopy.

    7. A novel multiphoton microscopy images segmentation method based on superpixel and watershed

      Weilin Wu, Jinyong Lin, Shu Wang, Yan Li, Mingyu Liu, Gaoqiang Liu, Jianyong Cai, Guannan Chen and Rong Chen

      Version of Record online: 19 APR 2016 | DOI: 10.1002/jbio.201600007

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      Processing procedure of the images segmentation method based on superpixels and watershed (MSW) segmentation algorithm for multiphoton microscopy image.

    8. Induction of neuronal activation by femtosecond-pulsed laser irradiation and its potential application for amyloid-β–induced toxicity assessment

      Seunghee Lee, Jonghee Yoon, Myunghwan Choi and Chulhee Choi

      Version of Record online: 19 APR 2016 | DOI: 10.1002/jbio.201600004

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      Direct optical stimulation at a neuron using femtosecond-pulsed laser can induce calcium elevation and ROS generation mediated by superoxide. We verified the applicability of such mechanism for the assessment of neuronal functionality in vehicle and Aβ-treated neurons. This work suggests promising applications of the potential for implementing such laser-induced neural activation for rapid and reliable drug screening.

    9. Investigating fibroblast cells under “safe” and “injurious” blue-light exposure by holographic microscopy

      Alejandro Calabuig, Martina Mugnano, Lisa Miccio, Simonetta Grilli and Pietro Ferraro

      Version of Record online: 18 APR 2016 | DOI: 10.1002/jbio.201500340

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      A study on the temporal evolution of cell morphology and volume during blue light exposure is reported. The results reveal a behaviour that is typical of necrotic cells, with early swelling and successive leakage of the intracellular liquids when the laser is set in the “injurious” operation. The approach may open the route to a deep investigation of light-cell interactions, with information about death pathways and threshold conditions between healthy and damaged cells when subjected to light-exposure.

    10. Activity assessment of root caries lesions with thermal and near-IR imaging methods

      Robert C. Lee, Cynthia L. Darling, Michal Staninec, Antonio Ragadio and Daniel Fried

      Version of Record online: 6 APR 2016 | DOI: 10.1002/jbio.201500333

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      The highly mineralized surface layer plays an important role in the dehydration process of remineralized dentin lesions. The PS-OCT algorithm for the automated assessment of remineralization successfully detected the highly mineralized surface layer on root caries lesions. Thermal imaging provided the best performance in root caries lesion activity assessment. Detection of remineralization with these novel methods has the potential to aid in the clinical assessment of root caries lesion activity.

    11. Non-invasive optical assessment of viscosity of middle ear effusions in otitis media

      Guillermo L. Monroy, Paritosh Pande, Ryan L. Shelton, Ryan M. Nolan, Darold R. Spillman Jr., Ryan G. Porter, Michael A. Novak and Stephen A. Boppart

      Version of Record online: 24 MAR 2016 | DOI: 10.1002/jbio.201500313

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      Middle ear disease is commonly evaluated with otoscopy, providing limited qualitative information about the tympanic membrane and middle-ear content. Here a method is presented to assess the general state of the middle ear with cross-sectional optical coherence tomography images that directly reveal the presence of a scattering effusion. Relative Stokes-Einstein coefficients are calculated for various suspensions and phantoms and both in vivo and ex vivo human middle ear effusion samples.

    12. Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy

      Molin Wongwattanakul, Chariya Hahnvajanawong, Patcharaporn Tippayawat, Sirinart Chio-Srichan, Chanvit Leelayuwat, Temduang Limpaiboon, Patcharee Jearanaikoon and Philip Heraud

      Version of Record online: 21 MAR 2016 | DOI: 10.1002/jbio.201500253

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      Cholangiocarcinoma (CCA), a cancer of bile duct epithelium, is a major health problem in Thailand especially in the northeast. Overall treatment outcomes have not shown much improvement because the disease is usually detected at an advanced stage and often shows chemotherapeutic resistance. High-throughput Fourier Transform Infrared (FTIR) microspectroscopy can be used for cell classification and has the potential to diagnose cancer and possibly predict chemo-response. This is the first study to demonstrate the use of FTIR microspectroscopy to assess chemo-response both in vitro and in vivo.

    13. Analysis of major elements in pigmented melanocytic chicken skin using laser-induced breakdown spectroscopy

      Jong Jin Lee, Youngmin Moon, Jung Hyun Han and Sungho Jeong

      Version of Record online: 21 MAR 2016 | DOI: 10.1002/jbio.201500343

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      In this study, concentration difference of the major elements depending on pigmentation level of skin was analysed by LIBS using the melanocytic skin of black silkie chicken. A clear difference in LIBS signal intensity or intensity ratio of the major elements with respect to pigmentation level was observed, demonstrating that LIBS can provide a reliable monitoring technique for selective and complete laser removal of darkly pigmented melanocytic lesion like nevus.

    14. RP-CARS reveals molecular spatial order anomalies in myelin of an animal model of Krabbe disease

      Giuseppe de Vito, Valentina Cappello, Ilaria Tonazzini, Marco Cecchini and Vincenzo Piazza

      Version of Record online: 15 MAR 2016 | DOI: 10.1002/jbio.201500305

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      A new microscopy technique (RP-CARS) is exploited to study a genetic model of the Krabbe disease, a fatal human demyelinating pathology. RP-CARS allows, in a completely label-free fashion, quantifying the molecular order within the axon myelin sheaths, highlighting the loss of myelin structural organization over sub-micrometric length scales during the disease progression. RP-CARS is a promising tool for the study of diseases affecting the structural organization of biological tissues.

    15. Visible Raman excitation laser induced power and exposure dependent effects in red blood cells

      Sunita Ahlawat, Nitin Kumar, Abha Uppal and Pradeep Kumar Gupta

      Version of Record online: 15 MAR 2016 | DOI: 10.1002/jbio.201500325

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      For increase in power of Raman excitation laser the oxy-Hb (low spin Fe3+) in RBC transitions to a high spin Fe2+ derivative (deoxy-Hb) due to photo-dissociation of oxygen from Hb. For long exposure durations, this deoxy-Hb irreversibly transitions back to a low spin Fe3+ derivative due to oxidation by the photo-dissociated oxygen. The RBCs deoxygenated via oxygen deprivation ( N2 purging) were very stable against any laser induced effect.

    16. Assessing the efficacy of coherent anti-Stokes Raman scattering microscopy for the detection of infiltrating glioblastoma in fresh brain samples

      Roberta Galli, Ortrud Uckermann, Achim Temme, Elke Leipnitz, Matthias Meinhardt, Edmund Koch, Gabriele Schackert, Gerald Steiner and Matthias Kirsch

      Version of Record online: 11 MAR 2016 | DOI: 10.1002/jbio.201500323

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      Typical feature of malignant brain tumors are infiltrating neoplastic cells, and their intraoperative detection would constitute a key prognostic feature. CARS microscopy is a label-free, non-invasive optical technique that offers the potential to visualize the tumor in situ and could therefore complement the existing intraoperative imaging tools. The morphochemistry of cells retrieved by CARS microscopy enables to discern the tumor from the surrounding normal brain tissue, thereby providing great potential towards tumor resection optimization.

    17. Motion-free endoscopic system for brain imaging at variable focal depth using liquid crystal lenses

      Arutyun Bagramyan, Tigran Galstian and Armen Saghatelyan

      Version of Record online: 8 MAR 2016 | DOI: 10.1002/jbio.201500261

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      An imaging system is introduced, based on gradient index and electrically tunable liquid crystal lenses, allowing motion-free imaging of neural circuitry at variable focal depth in vivo. Electrical tuning of the liquid crystal lens at constant voltage, but variable frequency enables about 75 µm focal shift allowing interrogation of morpho-functional properties of neural networks in various deep brain regions.

    18. Fiber bragg grating sensor based device for simultaneous measurement of respiratory and cardiac activities

      K. Chethana, A. S. Guru Prasad, S. N. Omkar and S. Asokan

      Version of Record online: 4 MAR 2016 | DOI: 10.1002/jbio.201500268

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      This paper reports a novel optical ballistocardiography technique, which is intrinsically safe and non-invasive for the simultaneous measurement of cardiac and respiratory activities using a Fiber Bragg Grating Heart Beat Device. The results of this work offers capabilities of monitoring nascent morphology of cardiac and breathing activity, heart rate variability, heart beat rhythm, etc., which can assist in early clinical diagnosis of many conditions associated with heart and lung malfunctioning.

    19. A comparative evaluation of diffuse reflectance and Raman spectroscopy in the detection of cervical cancer

      Rubina Shaikh, Vasumathi G. Prabitha, Tapas Kumar Dora, Supriya Chopra, Amita Maheshwari, Kedar Deodhar, Bharat Rekhi, Nita Sukumar, C. Murali Krishna and Narayanan Subhash

      Version of Record online: 1 MAR 2016 | DOI: 10.1002/jbio.201500248

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      This clinical study examines the relative merits of Raman and diffuse reflectance spectroscopy for in vivo detection of cervical cancer by processing the spectra recorded from the same sites in patients using principal component and linear discriminant analysis. Raman spectroscopy system that shows slightly improved diagnostic accuracy is more preferred in centralised facilities. But the advantages of affordability, ease of use and portability make the diffuse reflectance spectroscopy system more suited for community screening in low-resource settings.

    20. Multimodal optical analysis of meningioma and comparison with histopathology

      Marc Zanello, Fanny Poulon, Pascale Varlet, Fabrice Chretien, Felipe Andreiuolo, Mélanie Pages, Ali Ibrahim, Johan Pallud, Edouard Dezamis, Georges Abi-Lahoud, François Nataf, Baris Turak, Bertrand Devaux and Darine Abi-Haidar

      Version of Record online: 12 FEB 2016 | DOI: 10.1002/jbio.201500251

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      This study explores one- and two-photon microscopy abilities to help neurosurgeons in refining the margins of resection of meningioma. Two-photon autofluorescence gives images near to the gold standard histopathology and benign meningiomas exhibited less autofluorescence and longer lifetime values than aggressive meningiomas. Two-photon microscopy can give real-time information on the histopathological structure of the tissue (meningioma, infiltrated dura mater, healthy dura mater) encountered during resection.


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