Cancer Cytopathology

Cover image for Vol. 123 Issue 6

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Celeste N. Powers, MD, PhD

Impact Factor: 3.354

ISI Journal Citation Reports © Ranking: 2014: 16/75 (Pathology); 81/211 (Oncology)

Online ISSN: 1934-6638

Associated Title(s): Cancer, CA: A Cancer Journal for Clinicians


  1. 1 - 24
  1. Review Articles

    1. The Cell-CT 3-dimensional cell imaging technology platform enables the detection of lung cancer using the noninvasive LuCED sputum test

      Michael G. Meyer, Jon W. Hayenga, Thomas Neumann, Rahul Katdare, Chris Presley, David E. Steinhauer, Timothy M. Bell, Christy A. Lancaster and Alan C. Nelson

      Article first published online: 6 JUL 2015 | DOI: 10.1002/cncy.21576

      Detection of lung cancer by the LuCED test is based on high-resolution 3-dimensional imaging of cells from sputum using a new analytical device called Cell-CT. LuCED demonstrates the high-sensitivity detection of cancer cells with excellent specificity and has the potential to be used as a primary screener or used adjunctively with low-dose x-ray computed tomography.

  2. Original Articles

    1. Automated 3-dimensional morphologic analysis of sputum specimens for lung cancer detection: Performance characteristics support use in lung cancer screening

      David C. Wilbur, Michael G. Meyer, Chris Presley, Ralph W. Aye, Paul Zarogoulidis, Douglas W. Johnson, Nir Peled and Alan C. Nelson

      Article first published online: 6 JUL 2015 | DOI: 10.1002/cncy.21565

      Three-dimensional scanning of cells can be used to evaluate routinely obtained sputum specimens. Automated rare event analysis and increased morphologic discriminatory power combined with manual digital review demonstrates the potential to improve sensitivity and specificity to make lung cancer screening possible.

    2. Cytopathologic features of epithelioid inflammatory myofibroblastic sarcoma with correlation of histopathology, immunohistochemistry, and molecular cytogenetic analysis

      Jen-Chieh Lee, Jiann-Ming Wu, Jau-Yu Liau, Hsuan-Ying Huang, Cheng-Yu Lo, I-Shiow Jan, Jason L. Hornick and Xiaohua Qian

      Article first published online: 2 JUL 2015 | DOI: 10.1002/cncy.21558

      Epithelioid inflammatory myofibroblastic sarcoma is an aggressive variant of inflammatory myofibroblastic tumor. The cytologic features recapitulate its histologic characteristics. Diagnosis requires a high index of suspicion as well as confirmatory immunohistochemistry and molecular studies.

    3. Use of FTA cards for the storage of breast carcinoma nucleic acid on fine-needle aspiration samples

      Anna Lucia Peluso, Anna Maria Cascone, Lucrezia Lucchese, Immacolata Cozzolino, Antonio Ieni, Chiara Mignogna, Stefano Pepe and Pio Zeppa

      Article first published online: 29 JUN 2015 | DOI: 10.1002/cncy.21577

      The preservation and storage of nucleic acids is important for DNA molecular techniques, and therefore the material obtained by fine-needle aspiration needs to be emphasized. FTA cards can be conveniently used for the storage of breast carcinoma cells obtained by fine-needle aspiration, thereby providing a reliable alternative to traditional methods.

    4. Use of BRAF v600e immunocytochemistry on FNA direct smears of papillary thyroid carcinoma

      Sara E. Wobker, Lawrence T. Kim, Trevor G. Hackman and Leslie G. Dodd

      Article first published online: 16 JUN 2015 | DOI: 10.1002/cncy.21575

      The use of immunostaining with the anti-BRAF V600E antibody VE1 is possible on fine-needle aspirate direct smears. The staining process will require further optimization for routine use.

    5. Effective clinical practices for improved FNA biopsy cell block outcomes

      Brian T. Collins, Telly C. Garcia and Jena B. Hudson

      Article first published online: 16 JUN 2015 | DOI: 10.1002/cncy.21573

      At least 1 dedicated fine-needle aspiration (FNA) biopsy pass appears to improve cell block quality and the terminal FNA biopsy smear contributes significantly to obtaining a high-quality cell block. The performance of dedicated FNA biopsies and the use of high-quality aspirate smears are effective clinical practices with which to improve cell block outcomes.

  3. Commentary

    1. Comments on the recently published “Guidelines for the cytopathologic diagnosis of epithelioid and mixed-type malignant mesothelioma”

      Anders Hjerpe and Katalin Dobra

      Article first published online: 10 JUN 2015 | DOI: 10.1002/cncy.21563

      Recently published guidelines describe how a specific diagnosis based on effusions can be obtained in most patients with malignant mesothelioma. The high positive predictive value makes biopsy sampling redundant in these patients and allows an earlier diagnosis.

    2. US Food and Drug Administration regulatory oversight of laboratory-developed tests: Commentary on the draft guidance

      Mary K. Sidawy

      Article first published online: 4 JUN 2015 | DOI: 10.1002/cncy.21566

      In 1976, Congress introduced the Medical Device Amendments to regulate medical devices intended for use in humans. The definition of “device” encompasses diagnostic devices manufactured by manufacturers as well as laboratory-developed tests (LDTs). To ensure that LDTs are safe and effective, on October 3, 2014, the US Food and Drug Administration issued a draft document outlining a plan to enforce its authority. A second draft document outlines the laboratory requirements for reporting their LDTs and adverse events related to testing.

  4. Original Articles

    1. Digital gene expression profiling of a series of cytologically indeterminate thyroid nodules

      Riccardo Giannini, Liborio Torregrossa, Stefano Gottardi, Lorenzo Fregoli, Nicla Borrelli, Mauro Savino, Elisabetta Macerola, Paolo Vitti, Paolo Miccoli and Fulvio Basolo

      Article first published online: 29 MAY 2015 | DOI: 10.1002/cncy.21564

      Gene expression profiling may improve diagnostic accuracy in indeterminate fine-needle aspiration cytology samples. The current data suggest that preoperative gene expression profiling may be helpful in the classification of thyroid nodules.

  5. Help Desk

  6. Original Articles

    1. The impact of the new proposed Papanicolaou Society of Cytopathology terminology for pancreaticobiliary cytology in endoscopic US-FNA: A single-Institutional experience

      Mauro Ajaj Saieg, Valerie Munson, Shanna Colletti and Aziza Nassar

      Article first published online: 20 MAY 2015 | DOI: 10.1002/cncy.21559

      The application of the new proposed terminology for pancreaticobiliary cytology has a greater impact on specimens previously classified as atypical and suspicious. Its broad use may ultimately contribute to a more standardized therapy for patients with pancreatic disease.

    2. Efficiency of EGFR mutation analysis for small microdissected cytological specimens using multitech DNA extraction solution

      Seo Young Oh and Hoon Taek Lee

      Article first published online: 11 MAY 2015 | DOI: 10.1002/cncy.21550

      The MultiTech DNA extraction solution is a reliable medium that improves the resolution of cell morphology during microdissection. The results of the current study indicate that it is particularly useful in epidermal growth factor receptor mutations of samples containing a small number of cells.

  7. Review Articles

    1. Molecular markers: Implications for cytopathology and specimen collection

      Paul A. VanderLaan

      Article first published online: 11 MAY 2015 | DOI: 10.1002/cncy.21560

      In this era of personalized medicine, testing for a wide array of relevant molecular markers has become an increasingly important component in the diagnostic, prognostic, and therapeutic workup of cytology specimens. The recognition of these established and emerging molecular markers by cytopathologists is an important step toward realizing the promise of personalized medicine.

  8. Original Articles

    1. Poor cell block adequacy rate for molecular testing improved with the addition of Diff-Quik–stained smears: Need for better cell block processing

      Shuko Harada, Emmanuel Agosto-Arroyo, Jessica A. Levesque, Evan Alston, Karen M. Janowski, Gina M. Coshatt and Isam A. Eltoum

      Article first published online: 8 MAY 2015 | DOI: 10.1002/cncy.21561

      The objective of this study is to assess the frequency and types of samples deemed insufficient for lung cancer molecular testing (quantity not sufficient [QNS]). For cases rejected for the inadequacy of cell blocks of cytology specimens, air-dried, Diff-Quik–stained smears have been reviewed and used. The QNS rate for small biopsy specimens is 35.9% (28 of 78), whereas 64% (16 of 25) of cytology cases ordered on CBs are rejected. In combination with Diff-Quik–stained smears, the QNS rate for cytology specimens is 32% (8 of 25). The utilization of Diff-Quik–stained smears for molecular testing has improved the adequacy of cytologic samples, and it provides a minimally invasive alternative to surgical biopsy for molecular analysis.

    2. History of high-risk HPV and Pap test results in a large cohort of patients with invasive cervical carcinoma: Experience from the largest women's hospital in China

      Xiang Tao, Christopher C. Griffith, Xiangrong Zhou, Zhiheng Wang, Yabin Yan, Zaibo Li and Chengquan Zhao

      Article first published online: 8 MAY 2015 | DOI: 10.1002/cncy.21545

      In the current study, the authors explore prior high-risk human papillomavirus and Papanicolaou testing within 1 year of the diagnosis of cervical cancer in the largest women's hospital in China. Both high-risk human papillomavirus testing and Papanicolaou cytology appear to have similar negative rates in this population and fewer women were found to have negative testing results with both testing modalities.

    3. Prior high-risk HPV testing and Pap test results for 427 invasive cervical cancers in China's largest CAP-certified laboratory

      Baowen Zheng, Zaibo Li, Christopher C. Griffith, Shanshan Yan, Congde Chen, Xiangdong Ding, Xiaoman Liang, Huaitao Yang and Chengquan Zhao

      Article first published online: 8 MAY 2015 | DOI: 10.1002/cncy.21557

      This large study from China investigates prior human papillomavirus and Papanicolaou test results for patients with invasive cervical carcinoma from a population of women without access to an established screening program. The data demonstrate a considerable prior high-risk human papillomavirus–negative rate and a lower prior Papanicolaou-negative rate for these patients.

    4. Malignant risk stratification of thyroid FNA specimens with indeterminate cytology based on molecular testing

      Svetlana Paskaš, Jelena Janković, Vladan Živaljević, Svetislav Tatić, Vesna Božić, Aleksandra Nikolić, Dragica Radojković, Svetlana Savin and Dubravka Cvejić

      Article first published online: 27 APR 2015 | DOI: 10.1002/cncy.21554

      Based on the BRAF V600E mutation status, galectin-3 protein expression, and microRNA-221/222 expression, a predictor model distinguishing benign fine-needle aspiration samples with indeterminate cytology from malignant ones has been developed.

  9. Commentary

    1. Evolving role of FNA biopsy in diagnosing lymphoma: Past, present, and future

      Nancy P. Caraway

      Article first published online: 27 APR 2015 | DOI: 10.1002/cncy.21551

      Despite being challenged more than a decade ago, fine-needle aspiration biopsy continues to have a valuable role in diagnosing lymphomas. An updated lymphoma classification and several advances in technologies have aided in its increased acceptance and use; however, now is the time to integrate fine-needle aspiration biopsy with ancillary studies and obtain material for potential molecular testing in the routine workup of lymphomas to ensure its continued use in the future.

  10. Erratum

    1. You have free access to this content
  11. Original Articles

    1. Non-16/18 high-risk HPV infection predicts disease persistence and progression in women with an initial interpretation of LSIL

      Yasmin A. Lyons, Aparna A. Kamat, Haijun Zhou, Dina R. Mody, Mary R. Schwartz, Christopher Hobday and Yimin Ge

      Article first published online: 22 APR 2015 | DOI: 10.1002/cncy.21549

      Non-16/18 high-risk human papillomavirus genotypes strongly predict persistent cervical lesions and progression to high-grade lesions in women with an initial cytology interpretation of a low-grade squamous intraepithelial lesion.

  12. Clinician's Corner

  13. Original Articles

    1. Multiplex sequencing for EZH2, CD79B, and MYD88 mutations using archival cytospin preparations from B-cell non-Hodgkin lymphoma aspirates previously tested for MYC rearrangement and IGH/BCL2 translocation

      Gilda da Cunha Santos, Mauro Ajaj Saieg, Hyang Mi Ko, William R. Geddie, Scott L. Boerner, Kenneth J. Craddock, Michael Crump and Denis Bailey

      Article first published online: 23 MAR 2015 | DOI: 10.1002/cncy.21535

      Cytospin preparations (CP) stored at −20°C for up to 6 years are a reliable source of high-quality genomic material for multiplex sequencing. Successful detection of EZH2 (Y641), CD79B (Y196), and MYD88 (L265) mutations can be achieved using material scraped from archival CP obtained from B-cell non-Hodgkin lymphoma aspirates of cases previously tested for MYC rearrangement and IGH/BCL2 translocation.

  14. Erratum

    1. You have free access to this content
  15. Original Articles

    1. Microphthalmia transcription factor immunohistochemistry for FNA biopsy of ocular malignant melanoma

      Carmen M. Perrino, Jeff F. Wang and Brian T. Collins

      Article first published online: 24 FEB 2015 | DOI: 10.1002/cncy.21531

      This study is an evaluation of immunohistochemistry in the workup of fine-needle aspiration biopsies of ocular malignant melanoma and especially the new marker microphthalmia transcription factor. In addition, we show that microphthalmia transcription factor immunohistochemistry may be reliably performed on alcohol-fixed, Papanicolaou-stained direct smears.


  1. 1 - 24