Anaplastic lymphoma kinase gene rearrangements in cytological samples of non-small cell lung cancer: Comparison with histological assessment
Agnese Proietti, Greta Alì, Serena Pelliccioni, Cristiana Lupi, Elisa Sensi, Laura Boldrini, Adele Servadio, Antonio Chella, Alessandro Ribechini, Federico Cappuzzo, Mario Miccoli and Gabriella Fontanini
Article first published online: 19 MAR 2014 | DOI: 10.1002/cncy.21418
Anaplastic lymphoma kinase (ALK) rearrangement is present in 2% to 7% of cases of non-small cell lung cancer. Fluorescence in situ hybridization is currently the elective method for their detection. Although this assay has been approved on histological samples, its usefulness in detecting ALK gene rearrangements in cytological specimens has not been assessed fully. The authors found that ALK gene rearrangement may be easily detected in cytological samples and particularly in direct smears. Moreover, cytological samples may be useful for ALK analysis when insufficient material is available in cell blocks or small biopsy samples.