Cancer Cytopathology

Cover image for Vol. 123 Issue 8

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Celeste N. Powers, MD, PhD

Impact Factor: 3.737

ISI Journal Citation Reports © Ranking: 2014: 16/75 (Pathology); 81/211 (Oncology)

Online ISSN: 1934-6638

Associated Title(s): Cancer, CA: A Cancer Journal for Clinicians


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  1. Original Articles

    1. Suspicious cytologic diagnostic category in endoscopic ultrasound-guided FNA of the pancreas: Follow-up and outcomes

      Evan A. Alston, Sejong Bae and Isam A. Eltoum

      Article first published online: 3 SEP 2015 | DOI: 10.1002/cncy.21611

      The current study assesses how the suspicious category in pancreatic cytology is followed in a large endoscopic ultrasound-guided fine-needle aspiration service, its outcomes, and the predictors that are likely to be associated with the subsequent diagnosis of a neoplastic process.

  2. Commentary

    1. BRAF testing and thyroid FNA

      Marc P. Pusztaszeri, Jeffrey F. Krane and William C. Faquin

      Article first published online: 3 SEP 2015 | DOI: 10.1002/cncy.21614

      The BRAF V600E mutation represents the single most specific marker of malignancy in thyroid nodules, and there are selected scenarios in which BRAF testing alone can be useful.

  3. Original Articles

    1. HPV infection and p16 promoter methylation as predictors of ASC-US/LSIL progression

      Hee Lee and Eun-Ju Lee

      Article first published online: 3 SEP 2015 | DOI: 10.1002/cncy.21615

      The results of the current study indicate that the majority of cases of atypical squamous cells of undetermined significance (ASC-US)/low-grade squamous intraepithelial lesion (LSIL) in a Korean population regressed to normal and only 6.7% progressed to high-grade squamous intraepithelial lesion when followed by regular cytology, and that 17.8% of ASC-US/LSIL cases were found to harbor cervical intraepithelial neoplasia of grade 2 or higher in their tissues on immediate tissue biopsy. Furthermore, this study provides evidence that human papillomavirus infection and p16 promoter methylation are valuable surrogate markers for disease progression from ASC-US/LSIL to high-grade squamous intraepithelial lesion.

    2. Variations in cancer centers' use of cytology for the diagnosis of small cell lung carcinoma in the National Cancer Data Base

      Ted Gansler, Stacey A. Fedewa, Chun Chieh Lin, Ahmedin Jemal and Elizabeth M. Ward

      Article first published online: 31 AUG 2015 | DOI: 10.1002/cncy.21610

      Cytology is an accurate, safe, cost-effective, and guideline-recommended method for the diagnosis of small cell lung carcinoma. However, cytology is used more than twice as frequently in National Cancer Institute-designated cancer centers relative to community cancers centers (31.3% vs 13.2% of small cell lung carcinoma cases, respectively).

  4. Review Articles

    1. You have full text access to this OnlineOpen article
      Preanalytic parameters in epidermal growth factor receptor mutation testing for non–small cell lung carcinoma: A review of cytologic series

      Gilda da Cunha Santos and Mauro Ajaj Saieg

      Article first published online: 19 AUG 2015 | DOI: 10.1002/cncy.21595

      Preanalytic parameters for epidermal growth factor receptor mutation testing are reviewed in non–small cell lung cancer using 4999 cytologic samples from 22 studies. A higher mutation detection rate and lower numbers of insufficient cases are observed for pleural effusions and lymph node samples obtained using endobronchial ultrasound-guided transbronchial needle aspiration compared with histologic specimens, and low cellularity and a lower percentage of tumor cells are associated with higher test failure rates. Future guidelines should consider the current data for specific recommendations regarding cytologic samples.

  5. Clinician's Corner

    1. Screening for Anal Cancer

      Irving E. Salit

      Article first published online: 13 AUG 2015 | DOI: 10.1002/cncy.21580

  6. Original Articles

    1. The use of FNA samples for whole-exome sequencing and detection of somatic mutations in breast cancer surgical specimens

      Han-Byoel Lee, Je-Gun Joung, Jisun Kim, Kyung-Min Lee, Han Suk Ryu, Hae-Ock Lee, Hyeong-Gon Moon, Woong-Yang Park, Dong-Young Noh and Wonshik Han

      Article first published online: 11 AUG 2015 | DOI: 10.1002/cncy.21599

      Fine-needle aspiration is feasible for collecting tumor samples that are sufficient for whole-exome sequencing analysis. The higher purity obtained using this method may make it more reliable for genomic studies.

    2. Diagnostic utility of phosphorylated signal transducer and activator of transcription 5 immunostaining in the diagnosis of mammary analogue secretory carcinoma of the salivary gland: A comparative study of salivary gland cancers

      Akihiko Kawahara, Tomoki Taira, Hideyuki Abe, Yorihiko Takase, Takashi Kurita, Eiji Sadashima, Satoshi Hattori, Ichio Imamura, Shinji Matsumoto, Hitomi Fujisaki, Kazunobu Sueyoshi, Jun Akiba and Masayoshi Kage

      Article first published online: 7 AUG 2015 | DOI: 10.1002/cncy.21594

      Initial screening by phosphorylated signal transducer and activator of transcription 5 immunohistochemistry/immunocytochemistry, combined with auxiliary fluorescence in situ hybridization confirmation, is a reliable, economical approach to identifying mammary analogue secretory carcinoma of the salivary gland.

    3. Correlation of p16 immunohistochemistry in FNA biopsies with corresponding tissue specimens in HPV-related squamous cell carcinomas of the oropharynx

      Jalal B. Jalaly, James S. Lewis Jr, Brian T. Collins, Xingyong Wu, Xiao-Jun Ma, Yuling Luo and Cory T. Bernadt

      Article first published online: 4 AUG 2015 | DOI: 10.1002/cncy.21600

      An evaluation of p16 immunohistochemistry of cell blocks from fine-needle aspiration biopsies compared with surgical pathology specimens of human papillomavirus-related oropharyngeal squamous cell carcinoma is reported. There is excellent correlation between the 2 methods using a cutoff of at least 15% focal confluent staining in cell blocks.

    4. Significance of what is not sampled: Characteristics of thyroid nonmicrocarcinomas (>1.0 cm) that were not targeted

      Karen E. Schoedel, Jenna Wolfe, Steven P. Hodak, Shane O. Lebeau, Linwah Yip, Sally E. Carty, Marina N. Nikiforova, Yuri E. Nikiforov and N. Paul Ohori

      Article first published online: 4 AUG 2015 | DOI: 10.1002/cncy.21603

      The significance of nontargeted nonmicrocarcinomas (>1 cm) of the thyroid gland is studied in patients undergoing fine-needle aspiration of other nodules preoperatively. These nonmicrocarcinomas are rare (0.6% of all resected cases), and the majority are low-grade carcinomas; the frequency of having one of the common mutations is similar to the frequency for thyroid carcinomas in general.

    5. Correlation of microbiologic culture and fine-needle aspiration cytology: A 14-year experience at a single institution

      Cecilia G. Clement, Natalie M. Williams-Bouyer, Ranjana S. Nawgiri and Vicki J. Schnadig

      Article first published online: 4 AUG 2015 | DOI: 10.1002/cncy.21590

      The correlation of cytologic diagnoses with the results of microbiological culture and close communication with microbiology personnel are important for patient care and education. Monitoring cytology-microbiology correlation is a valuable quality assurance tool.

    6. Precursor T-lymphoblastic lymphoma: Speedy diagnosis in FNA and effusion cytology by morphology, immunochemistry, and flow cytometry

      Poonam Bhaker, Anirban Das, Arvind Rajwanshi, Upasana Gautam, Amita Trehan, Deepak Bansal, Neelam Varma and Radhika Srinivasan

      Article first published online: 4 AUG 2015 | DOI: 10.1002/cncy.21584

      Precursor T-lymphoblastic lymphoma is a rare lymphoma presenting clinically in children and adolescents with a rapidly enlarging mediastinal mass, dyspnea, and cervical lymphadenopathy requiring a quick diagnosis. The rapid diagnosis of T-lymphoblastic lymphoma in cytologic specimens such as fine-needle aspiration samples and pleural effusions can be challenging and is best approached by supplementing cytomorphology with flow cytometric immunophenotyping and cell block immunocytochemistry.

  7. Clinician's Corner

  8. Original Articles

    1. Factors affecting the success of next-generation sequencing in cytology specimens

      Sinchita Roy-Chowdhuri, Rashmi S. Goswami, Hui Chen, Keyur P. Patel, Mark J. Routbort, Rajesh R. Singh, Russell R. Broaddus, Bedia A. Barkoh, Jawad Manekia, Hui Yao, L. Jeffrey Medeiros, Gregg Staerkel, Rajyalakshmi Luthra and John Stewart

      Article first published online: 31 JUL 2015 | DOI: 10.1002/cncy.21597

      Next-generation sequencing in cytology specimens is often challenging because of the limited sample volume. The failure of next-generation sequencing is multifactorial, and identifying these pre-analytical variables will allow better selection of cytology material and increase the frequency of successful testing.

    2. HPV DNA testing with cytology triage in cervical cancer screening: Influence of revealing HPV infection status

      Lyndsay Ann Richardson, Mariam El-Zein, Agnihotram V. Ramanakumar, Samuel Ratnam, Ghislain Sangwa-Lugoma, Adhemar Longatto-Filho, Marly Augusto Cardoso, Francois Coutlée, Eduardo L. Franco and The PEACHS (Pap Efficacy After Cervical HPV Status) Study Consortium

      Article first published online: 31 JUL 2015 | DOI: 10.1002/cncy.21596

      In the current study, knowledge of human papillomavirus positivity by cytotechnicians results in a reduction in the diagnostic accuracy of Papanicolaou cytology, contrary to expectations. Findings from this study underscore the importance of maintaining meticulous quality control practices for cytology, even when serving as a triage test subsequent to primary human papillomavirus testing.

    3. Uncommon BRAF mutations in the follicular variant of thyroid papillary carcinoma: New insights

      Esther Diana Rossi, Maurizio Martini, Tommaso Bizzarro, Sara Capodimonti, Tonia Cenci, Celestino Pio Lombardi, Alfredo Pontecorvi, Guido Fadda and Luigi Maria Larocca

      Article first published online: 31 JUL 2015 | DOI: 10.1002/cncy.21586

      Mutational analysis is reshaping the practice of fine-needle aspiration cytology for the diagnosis of thyroid nodules. Although BRAF mutations are the most common in thyroid cancer, rare mutations have been described and are mainly associated with the follicular variant.

    4. Epidermal growth factor receptor mutation status in cell-free DNA supernatant of bronchial washings and brushings

      Akihiko Kawahara, Chihiro Fukumitsu, Tomoki Taira, Hideyuki Abe, Yorihiko Takase, Kazuya Murata, Tomohiko Yamaguchi, Koichi Azuma, Hidenobu Ishii, Shinzo Takamori, Jun Akiba, Tomoaki Hoshino and Masayoshi Kage

      Article first published online: 31 JUL 2015 | DOI: 10.1002/cncy.21583

      Activating epidermal growth factor receptor mutations can be detected with cytology cell-free DNA extracted from the supernatant fluid of liquid-based samples.

  9. Cytopathology Help Desk

    1. Cytopathology meets basic science

      Elena Vigliar, Umberto Malapelle and Giancarlo Troncone

      Article first published online: 20 JUL 2015 | DOI: 10.1002/cncy.21578

  10. Correspondence

    1. You have full text access to this OnlineOpen article
      Reply to comparison of cervical cancer screening results among 256,648 women in multiple clinical practices

      Douglas S. Rabin, Amy J. Blatt, Ronald Kennedy, Ronald D. Luff and R. Marshall Austin

      Article first published online: 15 JUL 2015 | DOI: 10.1002/cncy.21570

  11. Review Articles

    1. The Cell-CT 3-dimensional cell imaging technology platform enables the detection of lung cancer using the noninvasive LuCED sputum test

      Michael G. Meyer, Jon W. Hayenga, Thomas Neumann, Rahul Katdare, Chris Presley, David E. Steinhauer, Timothy M. Bell, Christy A. Lancaster and Alan C. Nelson

      Article first published online: 6 JUL 2015 | DOI: 10.1002/cncy.21576

      Detection of lung cancer by the LuCED test is based on high-resolution 3-dimensional imaging of cells from sputum using a new analytical device called Cell-CT. LuCED demonstrates the high-sensitivity detection of cancer cells with excellent specificity and has the potential to be used as a primary screener or used adjunctively with low-dose x-ray computed tomography.

  12. Original Articles

    1. Automated 3-dimensional morphologic analysis of sputum specimens for lung cancer detection: Performance characteristics support use in lung cancer screening

      David C. Wilbur, Michael G. Meyer, Chris Presley, Ralph W. Aye, Paul Zarogoulidis, Douglas W. Johnson, Nir Peled and Alan C. Nelson

      Article first published online: 6 JUL 2015 | DOI: 10.1002/cncy.21565

      Three-dimensional scanning of cells can be used to evaluate routinely obtained sputum specimens. Automated rare event analysis and increased morphologic discriminatory power combined with manual digital review demonstrates the potential to improve sensitivity and specificity to make lung cancer screening possible.

    2. Use of FTA cards for the storage of breast carcinoma nucleic acid on fine-needle aspiration samples

      Anna Lucia Peluso, Anna Maria Cascone, Lucrezia Lucchese, Immacolata Cozzolino, Antonio Ieni, Chiara Mignogna, Stefano Pepe and Pio Zeppa

      Article first published online: 29 JUN 2015 | DOI: 10.1002/cncy.21577

      The preservation and storage of nucleic acids is important for DNA molecular techniques, and therefore the material obtained by fine-needle aspiration needs to be emphasized. FTA cards can be conveniently used for the storage of breast carcinoma cells obtained by fine-needle aspiration, thereby providing a reliable alternative to traditional methods.

    3. Use of BRAF v600e immunocytochemistry on FNA direct smears of papillary thyroid carcinoma

      Sara E. Wobker, Lawrence T. Kim, Trevor G. Hackman and Leslie G. Dodd

      Article first published online: 16 JUN 2015 | DOI: 10.1002/cncy.21575

      The use of immunostaining with the anti-BRAF V600E antibody VE1 is possible on fine-needle aspirate direct smears. The staining process will require further optimization for routine use.

    4. Effective clinical practices for improved FNA biopsy cell block outcomes

      Brian T. Collins, Telly C. Garcia and Jena B. Hudson

      Article first published online: 16 JUN 2015 | DOI: 10.1002/cncy.21573

      At least 1 dedicated fine-needle aspiration (FNA) biopsy pass appears to improve cell block quality and the terminal FNA biopsy smear contributes significantly to obtaining a high-quality cell block. The performance of dedicated FNA biopsies and the use of high-quality aspirate smears are effective clinical practices with which to improve cell block outcomes.

  13. Commentary

    1. US Food and Drug Administration regulatory oversight of laboratory-developed tests: Commentary on the draft guidance

      Mary K. Sidawy

      Article first published online: 4 JUN 2015 | DOI: 10.1002/cncy.21566

      In 1976, Congress introduced the Medical Device Amendments to regulate medical devices intended for use in humans. The definition of “device” encompasses diagnostic devices manufactured by manufacturers as well as laboratory-developed tests (LDTs). To ensure that LDTs are safe and effective, on October 3, 2014, the US Food and Drug Administration issued a draft document outlining a plan to enforce its authority. A second draft document outlines the laboratory requirements for reporting their LDTs and adverse events related to testing.


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