Cancer Cytopathology

Cover image for Vol. 122 Issue 7

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Celeste N. Powers, MD, PhD

Impact Factor: 4.434

ISI Journal Citation Reports © Ranking: 2012: 11/77 (Pathology); 45/197 (Oncology)

Online ISSN: 1934-6638

Associated Title(s): Cancer, CA: A Cancer Journal for Clinicians


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  1. Original Articles

    1. A minimum fluid volume of 75 mL is needed to ensure adequacy in a pleural effusion: A retrospective analysis of 2540 cases

      Lisa M. Rooper, Syed Z. Ali and Matthew T. Olson

      Article first published online: 24 JUL 2014 | DOI: 10.1002/cncy.21452

      A pleural effusion volume of ≥ 75 mL is required to eliminate the influence of specimen size on diagnostic adequacy. Above 75 mL, larger volumes further decrease the numbers of indeterminate or nondiagnostic results.

    2. The role of SOX11 immunostaining in confirming the diagnosis of mantle cell lymphoma on fine-needle aspiration samples

      Y. Helen Zhang, Joe Liu, Marilyn Dawlett, Ming Guo, Xiaoping Sun and Yun Gong

      Article first published online: 23 JUL 2014 | DOI: 10.1002/cncy.21465

      SOX11 immunostaining on fine-needle aspiration samples is highly accurate for mantle cell lymphoma. The staining can be used as a reliable adjunct to confirm mantle cell lymphoma.

    3. The role of cytopathology and cyst fluid analysis in the preoperative diagnosis and management of pancreatic cysts >3 cm

      Ivan Chebib, Kurt Yaeger, Mari Mino-Kenudson and Martha B. Pitman

      Article first published online: 17 JUL 2014 | DOI: 10.1002/cncy.21460

      Fine needle aspiration of pancreatic cysts >3 cm adds value to imaging studies, especially when compared with computed tomography. Cytology is more specific for the detection of malignancy than imaging for cysts >3 cm.

    4. Wilms tumor 1/Cytokeratin dual-color immunostaining reveals distinctive staining patterns in metastatic melanoma, metastatic carcinoma, and mesothelial cells in pleural fluids: An effective first-line test for the workup of malignant effusions

      James R. Conner, Edmund S. Cibas, Jason L. Hornick and Xiaohua Qian

      Article first published online: 17 JUL 2014 | DOI: 10.1002/cncy.21439

      Simple Wilms tumor 1 (WT1)/AE1/AE3 dual-color double immunohistochemical staining reveals 4 distinct staining patterns in various epithelioid malignant effusions, which can be used as a rapid and cost-effective first-line test for detecting the presence and determining the site of origin of metastases in malignant effusions.

    5. Lung cancer adrenal gland metastasis: Optimal fine-needle aspirate and touch preparation smear cellularity characteristics for successful theranostic next-generation sequencing

      Ferga C. Gleeson, Benjamin R. Kipp, Michael J. Levy, Jesse S. Voss, Michael B. Campion, Douglas M. Minot, Zheng J. Tu, Eric W. Klee, Konstantinos N. Lazaridis and Sarah E. Kerr

      Article first published online: 15 JUL 2014 | DOI: 10.1002/cncy.21464

      Cellular cytologic smears from adrenal gland metastases are suitable for next-generation sequencing in patients with advanced lung cancer. In the current study, the characteristics of acceptable smears are outlined for use in onsite adequacy evaluation.

    6. The low risk of precancer after a screening result of human papillomavirus-negative/atypical squamous cells of undetermined significance papanicolaou and implications for clinical management

      Julia C. Gage, Hormuzd A. Katki, Mark Schiffman, Philip E. Castle, Barbara Fetterman, Nancy E. Poitras, Thomas Lorey, Li C. Cheung, Catherine Behrens, Abha Sharma, Fang-Hui Zhao, Jack Cuzick, Zi Hua Yang and Walter K. Kinney

      Article first published online: 9 JUL 2014 | DOI: 10.1002/cncy.21463

    7. Pancreatic neuroendocrine tumors: Accurate grading with Ki-67 index on fine-needle aspiration specimens using the WHO 2010/ENETS criteria

      Jessica M. Farrell, Judy C. Pang, Grace E. Kim and Z. Laura Tabatabai

      Article first published online: 9 JUL 2014 | DOI: 10.1002/cncy.21457

      The precise and accurate grading of pancreatic neuroendocrine tumors is critical for predicting biologic behavior patient prognosis, and making informed decisions regarding patient management and treatment. Pancreatic neuroendocrine tumors can be accurately graded by analyzing the Ki-67 proliferation indices in fine-needle aspiration specimens.

    8. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens

      Yi-Zhen Liu, Yan-Yi Jiang, Bo-Shi Wang, Jia-Jie Hao, Li Shang, Tong-Tong Zhang, Jian Cao, Xin Xu, Qi-Min Zhan and Ming-Rong Wang

      Article first published online: 9 JUL 2014 | DOI: 10.1002/cncy.21461

      The 6-protein panel (TP53, Ki67, MCM6, MCM7, KIAA1522 and KIAA0317) is a potential biomarker for the early detection of lung cancer in bronchial brushings. The combinaion of cytology and the protein panel significantly improved the sensitivity of bronchial brushing examination for lung cancer detection

  2. Cytopathology Help Desk

    1. The patient-centered practice

      David B. Kaminsky

      Article first published online: 1 JUL 2014 | DOI: 10.1002/cncy.21450

  3. Original Articles

    1. Reliability of immunostaining using pan-melanoma cocktail, SOX10, and microphthalmia transcription factor in confirming a diagnosis of melanoma on fine-needle aspiration smears

      Jessica Clevenger, Cicily Joseph, Marilyn Dawlett, Ming Guo and Yun Gong

      Article first published online: 20 JUN 2014 | DOI: 10.1002/cncy.21458

      In the current study, SOX10 was found to have the highest detection rate for melanoma on cytology smears, followed by microphthalmia transcription factor (MITF) and pan-melanoma cocktail. The nuclear staining markers, SOX10 and MITF, appear to be more readily interpreted than pan-melanoma cocktail.

    2. Impact of touch preparations on core needle biopsies

      Leung Chu B. Tong, Dorota Rudomina, Natasha Rekhtman and Oscar Lin

      Article first published online: 19 JUN 2014 | DOI: 10.1002/cncy.21447

      Touch preparations used for rapid on-site assessment have the potential to impact the findings in core biopsies.

    3. Cytologic features of parathyroid fine-needle aspiration on ThinPrep preparations

      Shelley I. Odronic, Jordan P. Reynolds and Deborah J. Chute

      Article first published online: 18 JUN 2014 | DOI: 10.1002/cncy.21453

      The cytologic features of parathyroid tissue on ThinPrep slides differ from those found on smears. ThinPrep cases demonstrate lower cellularity, lack papillary architecture, and show a predominant microfollicular pattern compared with aspirate smears.

    4. Papanicolaou tests with coexisting squamous and glandular abnormalities

      Li Yan Khor, Fadi W. Abdul-Karim, Amanda E. Bruening, Dana K. Weber Noffsinger and Christine N. Booth

      Article first published online: 17 JUN 2014 | DOI: 10.1002/cncy.21448

      Dual Papanicolaou test interpretations with both squamous and glandular atypia were more often associated with squamous lesions on follow-up. Dual interpretations were associated with 9 coexisting squamous and glandular lesions on follow-up.

  4. Review Articles

    1. Nucleic acid-based assays for the detection of high-risk human papillomavirus: A technical review

      Jane S. Gibson

      Article first published online: 17 JUN 2014 | DOI: 10.1002/cncy.21451

      Nucleic acid-based high-risk human papillomavirus testing is essential to contemporary cervical cancer screening. An understanding of methodologies associated with human papillomavirus testing is important to the practice of laboratory medicine.

  5. Erratum

    1. You have free access to this content
  6. Original Articles

    1. CD117: A novel ancillary marker for papillary thyroid carcinoma in fine-needle aspiration biopsies

      Marc P. Pusztaszeri, Peter M. Sadow and William C. Faquin

      Article first published online: 11 JUN 2014 | DOI: 10.1002/cncy.21437

      To investigate previous reports of CD117 down-regulation in papillary thyroid carcinoma (PTC), CD117 expression is assessed in cytologic and corresponding histologic samples of PTC (n = 35) compared with a control group of benign thyroid nodules (n = 30). CD117 is expressed by benign thyroid nodules and non-neoplastic follicular epithelium but is absent or nearly absent in PTC, suggesting that CD117 may be useful as an ancillary marker in the evaluation of PTC on thyroid fine-needle aspiration biopsy.

    2. Molecular Characterization of 54 cases of false-negative fine-needle aspiration among 1347 papillary thyroid carcinomas

      Agnese Proietti, Nicla Borrelli, Riccardo Giannini, Rossana Romani, Giancarlo Di Coscio, Francesca Quilici, Teresa Rago, Paolo Miccoli, Paolo Vitti and Fulvio Basolo

      Article first published online: 9 JUN 2014 | DOI: 10.1002/cncy.21454

      The rate of false-negative cases on thyroid fine-needle aspiration was 1.3% (18 of 1347 cases) in the referral center. The addition of molecular analysis decreased the false-negative rate to 0.4% (5 of 1347 cases). These data suggest that the addition of BRAF and Ras mutation preoperative assessment may be helpful but the cost benefit must be carefully evaluated.

    3. You have full text access to this OnlineOpen article
      Thyroid fine-needle aspiration cytology: Performance data of neoplastic and malignant cases as identified from 1558 responses in the ASCP Non-GYN Assessment program thyroid fine-needle performance data

      Stan G. Eilers, Paula LaPolice, Perkins Mukunyadzi, Umesh Kapur, Amy Wendel Spiczka, Ajay Shah, Husain Saleh, Adebowale Adeniran, Amberly Nunez, Indra Balachandran, Jennifer J. Clark and Larry Lemon

      Article first published online: 9 JUN 2014 | DOI: 10.1002/cncy.21440

      Data from 1558 responses in the American Society for Clinical Pathology Non-GYN Assessment program for thyroid fine-needle aspirates from thyroid neoplasms and malignancies were evaluated for the correct diagnostic interpretation as well as classifying the incorrect responses into treatment-based groups (nonsurgical benign thyroid nodule, thyroid neoplasm, or malignancy). Participants generally performed well, except for the entities of follicular neoplasm and follicular variant of papillary carcinoma, which were challenging diagnoses to program participants.

    4. The value of the “suspicious for urothelial carcinoma” cytology category: A correlative study of 4 years including 337 patients

      Tuyet Nhung Ton Nu, Wassim Kassouf, Babak Ahmadi-Kaliji, Michele Charbonneau, Manon Auger and Fadi Brimo

      Article first published online: 6 JUN 2014 | DOI: 10.1002/cncy.21449

      A diagnosis of “suspicious for urothelial carcinoma” is likely similar to the recently suggested “atypical urothelial cells, cannot rule out high-grade carcinoma” category. It also carries a significant risk of harboring high-grade urothelial carcinoma.

    5. FNAB of benign thyroid nodules with papillary hyperplasia: A cytological and histological evaluation

      Marc P. Pusztaszeri, Jeffrey F. Krane, Edmund S. Cibas, Gilbert Daniels and William C. Faquin

      Article first published online: 2 JUN 2014 | DOI: 10.1002/cncy.21441

      A subset of benign thyroid nodules with papillary hyperplasia is misinterpreted on fine-needle aspiration biopsy as “suspicious for malignancy” or as “malignant,” potentially resulting in unnecessary surgery. These cases are characterized histologically by a high proportion of papillary hyperplasia and cytologically by papillary structures, variable nuclear atypia, small nuclear size, and cytoplasmic pigment.

    6. Human papillomavirus testing in young women: Clinical outcomes of human papillomavirus triage in a UK cervical screening program

      Michael McKenna and Mary M. McMenamin

      Article first published online: 2 JUN 2014 | DOI: 10.1002/cncy.21444

      Colposcopy referrals increased significantly with the introduction of human papillomavirus triage. In women with borderline cytology or mild dyskaryosis who tested positive for human papillomavirus, there was no significant difference noted in the rate of biopsy-confirmed cases of cervical intraepithelial neoplasia of type 2 or higher or type 3 or higher between women aged < 25 years and those aged ≥ 25 years.

  7. Erratum

    1. You have free access to this content
  8. Original Articles

    1. Factors associated with reduced accuracy in Papanicolaou tests for patients with invasive cervical cancer

      Lichao Zhao, Nicolas Wentzensen, Roy R. Zhang, S. Terence Dunn, Michael A. Gold, Sophia S. Wang, Mark Schiffman, Joan L. Walker and Rosemary E. Zuna

      Article first published online: 28 MAY 2014 | DOI: 10.1002/cncy.21443

      Papanicolaou tests from women with invasive cervical cancer, particularly squamous cancers, have a higher rate of unsatisfactory and limited-quality samples. Human papillomavirus testing of unsatisfactory ThinPrep Pap tests in women aged > 30 years can identify high-risk women in this scenario.

    2. The value of second opinion in thyroid cytology: A review

      Rene Gerhard and Scott L. Boerner

      Article first published online: 28 MAY 2014 | DOI: 10.1002/cncy.21436

      Second opinion review of thyroid fine-needle aspiration improves diagnostic accuracy and is better supported by clinical follow-up. Changes in the clinical management occur in a significant number of patients with thyroid nodules after the results of second-opinion diagnoses.

    3. What is the malignancy risk for atypia of undetermined significance?: Three years' experience at a University Hospital in Turkey

      Pinar Uyar Gocun, Esra Karakus, Pinar Bulutay, Mujde Akturk, Murat Akin and Aylar Poyraz

      Article first published online: 28 MAY 2014 | DOI: 10.1002/cncy.21434

      A multimodal approach (clinical, radiologic, and cytopathologic) is very important in the management of thyroid nodules diagnosed as atypia of undetermined significance (AUS)/follicular lesion of undetermined significance. Therefore, recommendation for repeat fine-needle aspiration following an initial diagnosis of AUS should be based on a multimodal approach for each particular patient.

    4. Programmed cell death 4 and microRNA 21 inverse expression is maintained in cells and exosomes from ovarian serous carcinoma effusions

      Rocco Cappellesso, Andrea Tinazzi, Thomas Giurici, Francesca Simonato, Vincenza Guzzardo, Laura Ventura, Marika Crescenzi, Silvia Chiarelli and Ambrogio Fassina

      Article first published online: 28 MAY 2014 | DOI: 10.1002/cncy.21442

      PDCD4 and miR-21 are involved in ovarian serous carcinma oncogenesis. The transfer of miR-21 by exosomes could promote oncogenic transformation in target cells distant from the primary tumor without direct colonization by cancer cells and could be used as diagnostic tool.

    5. Accuracy of bronchial brush and wash specimens prepared by the ThinPrep method in the diagnosis of pulmonary small cell carcinoma

      Cheng Wang, Qiuli Duan, Margaret Kelly and Máire A. Duggan

      Article first published online: 13 MAY 2014 | DOI: 10.1002/cncy.21435

      In the diagnosis of pulmonary small cell carcinoma, unsatisfactory brush and wash Thinprep specimens are infrequent. Both have moderate sensitivity and high specificity, positive predictive value, and negative predictive value, and abnormal cell features resemble those seen in conventional preparations.

  9. Clinician's Corner

  10. Review Articles

    1. Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA): An overview and update for the cytopathologist

      Paul A. VanderLaan, Helen H. Wang, Adnan Majid and Erik Folch

      Article first published online: 23 APR 2014 | DOI: 10.1002/cncy.21431

      Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) allows access to mediastinal, hilar, and parenchymal lung tissue in a safe and minimally invasive fashion, frequently in an outpatient setting. The cytologic materials obtained by EBUS-TBNA have proven excellent for the diagnosis of lung cancer and for ancillary testing in lung cancer.

  11. Original Articles

    1. Evaluation of intraoperative cytological assessment of bone resection margins in patients with oral squamous cell carcinoma

      Markus Nieberler, Patrick Häusler, Enken Drecoll, Mechthild Stoeckelhuber, Herbert Deppe, Frank Hölzle, Andreas Kolk, Klaus-Dietrich Wolff, Marco Rainer Kesting and Gregor Weirich

      Article first published online: 18 APR 2014 | DOI: 10.1002/cncy.21428

      The current study evaluates the intraoperative cytological assessment of bone resection margins to control resection status in patients with bone-infiltrating oral squamous cell carcinomas. The work demonstrates that intraoperative cytological assessment of bone resection margins could supplement the intraoperative assessment of soft tissue margins through the immediate microscopic control of bony resection margins, enabling a guided reresection of positive bone margins during a surgical intervention to optimize surgical concepts, especially for patients with carcinoma, who receive primary reconstruction with osteo-(myo-)cutaneous microvascular transplants.

    2. Targeted multiprobe fluorescence in situ hybridization analysis for elucidation of inconclusive pancreatobiliary cytology

      Tatjana Vlajnic, Gina Somaini, Spasenija Savic, Audrey Barascud, Bruno Grilli, Michelle Herzog, Ellen C. Obermann, Brittany J. Holmes, Syed Z. Ali, Lukas Degen and Lukas Bubendorf

      Article first published online: 18 APR 2014 | DOI: 10.1002/cncy.21429

      This study shows that multitarget fluorescence in situ hybridization (FISH) is diagnostically helpful in cases of inconclusive pancreatobiliary cytology (including atypical and suspicious categories).


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