Interobserver reproducibility of cytologic p16INK4a/Ki-67 dual immunostaining in human papillomavirus-positive women
Maria Benevolo, Elena Allia, Daniela Gustinucci, Francesca Rollo, Simonetta Bulletti, Elena Cesarini, Basilio Passamonti, Maria Rosaria Giovagnoli, Elisabetta Carico, Francesca M. Carozzi, Alessandra Mongia, Giulia Fantacci, Massimo Confortini, Teresa Rubino, Cristina Fodero, Sonia Prandi, Natalina Marchi, Angelo Farruggio, Anna Coccia, Luigia Macrì, Bruno Ghiringhello, Guglielmo Ronco, Emma Bragantini, Enzo Polla, Vincenzo Maccallini, Giovanni Negri, Paolo Giorgi Rossi and for the New Technologies for Cervical Cancer Screening 2 (NTCC2) Working Group
Version of Record online: 7 DEC 2016 | DOI: 10.1002/cncy.21800
In human papillomavirus (HPV)-based cervical cancer screening, dual-staining for cyclin-dependent kinase inhibitor 2A (p16ink4a) and Ki-67 has been proposed as a biomarker for triaging women who are positive for HPV DNA. Staining for p16ink4a/Ki-67 has good reproducibility between readers from 9 different laboratories, confirming its robustness, which is a necessary requisite for its introduction into cervical cancer screening.