Cover image for Vol. 71 Issue 5

Edited By: Bruce L. Goode, Makoto Kinoshita, Pekka Lappalainen

Online ISSN: 1949-3592

Virtual Issue

Editor’s Choice Virtual Issue

Research Articles:

Isolation of intraflagellar transport trains
Caterina Mencarelli, Aaron Mitchell, Roberto Leoncini, Joel Rosenbaum, and Pietro Lupetti

An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility
Megha Vaman Rao, Pei-Hsuan Chu, Klaus Michael Hahn, and Ronen Zaidel-Bar

Biochemical evidence that human EB1 does not bind preferentially to the microtubule seam
Emily L. Alberico (nee Ottenweller), Daniel F. Lyons, Ryan J. Murphy, Julia T. Philip, Aranda R. Duan, John J. Correia, and Holly V. Goodson

Establishing a novel knock-in mouse line for studying neuronal cytoplasmic dynein under normal and pathologic conditions
Jun Zhang, Alison E. Twelvetrees, Jacob E. Lazarus, Kiev R. Blasier, Xuanli Yao, Nirja A. Inamdar, Erika L. F. Holzbaur, K.Kevin Pfister and Xin Xiang

Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis
Akihiro C. E. Shibata, Limin H. Chen, Rie Nagai, Fumiyoshi Ishidate, Rahul Chadda, Yoshihiro Miwa, Keiji Naruse, Yuki M. Shirai, Takahiro K. Fujiwara and Akihiro Kusumi

Conserved structural motifs in the central pair complex of eukaryotic flagella
Blanca I. Carbajal-González, Thomas Heuser, Xiaofeng Fu, Jianfeng Lin, Brandon W. Smith, David R. Mitchell and Daniela Nicastro

Regulation of integrin trafficking, cell adhesion, and cell migration by WASH and the Arp2/3 complex
Steve N. Duleh and Matthew D. Welch

CdGAP regulates cell migration and adhesion dynamics in two-and three-dimensional matrix environments
Duncan Wormer, Nicholas O. Deakin and Christopher E. Turner

Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone
Akihiro C. E. Shibata, Takahiro K. Fujiwara, Limin Chen, Kenichi G. N. Suzuki, Yoshiro Ishikawa, Yuri L. Nemoto, Yoshihiro Miwa, Ziya Kalay, Rahul Chadda, Keiji Naruse and Akihiro Kusumi

Structure and activity of full-length formin mDia1
Sankar Maiti, Alphee Michelot, Christopher Gould, Laurent Blanchoin, Olga Sokolova and Bruce L. Goode

Analyses of functional domains within the PF6 protein of the central apparatus reveal a role for PF6 sub-complex members in regulating flagellar beat frequency
Daniel J. Goduti and Elizabeth F. Smith

The structural heterogeneity of radial spokes in cilia and flagella is conserved
Jianfeng Lin, Thomas Heuser, Blanca I. Carbajal-González, Kangkang Song and Daniela Nicastro

Technique Article:

Quantification of MAP and molecular motor activities on geometrically controlled microtubule networks
Didier Portran, Jeremie Gaillard, Marylin Vantard and Manuel Thery

Actin and Regulatory Proteins Virtual Issue

The actin cytoskeleton has a fundamental role in numerous cellular processes from cell migration and morphogenesis to vesicle trafficking and cell division. Consequently, the actin cytoskeleton is also a key player in many developmental and physiological processes in multicellular organisms, and abnormalities in the regulation of actin dynamics are typical for many pathological states such as in cancer and during infection processes of several pathogenic bacteria and viruses.

During the past three years, many important articles concerning biochemistry and biology of actin and actin-associated proteins have been published in Cytoskeleton. These publications have provided important new insights into the structure and dynamics of monomeric and filamentous actin, role of actin isoforms in multicellular organisms, and regulation of the organization and dynamics of the actin cytoskeleton by various actin-binding proteins. For your convenience, we have gathered a selection of recent papers published in Cytoskeleton on this topic and make them freely accessible to you through this Virtual Issue.

Review Articles:

Tropomodulins: Pointed-end capping proteins that regulate actin filament architecture in diverse cell types
Sawako Yamashiro, David S. Gokhin, Sumiko Kimura, Roberta B. Nowak and Velia M. Fowler

Cease-fire at the leading edge: New perspectives on actin filament branching, debranching, and cross-linking
Casey A. Ydenberg, Benjamin A. Smith, Dennis Breitsprecher, Jeff Gelles and Bruce L. Goode

Rab8 GTPase as a regulator of cell shape
Johan Peränen

Extracellular inhibitors, repellents, and semaphorin/plexin/MICAL-mediated actin filament disassembly
Ruei-Jiun Hung and Jonathan R. Terman

Conformational dynamics of actin: Effectors and implications for. biological function
Gábor Hild, Beáta Bugyi, Miklós Nyitrai

Actin-depolymerizing factor homology domain: A conserved fold performing diverse roles in cytoskeletal dynamics.
Minna Poukkula, Elena Kremneva, Martina Serlachius and Pekka Lappalainen

Research Articles:

Submembranous septins as relatively stable components of actin-based membrane skeleton
Akari Hagiwara, Yasuhiro Tanaka, Rie Hikawa, Nobuhiro Morone, Akihiro Kusumi, Hiroshi Kimura and Makoto Kinoshita

Different positions of tropomyosin isoforms on actin filament are determined by specific sequences of end-to-end overlaps
Małgorzata Śliwińska, Magdalena Żukowska, Danuta Borys and Joanna Moraczewska

Cofilin is required for organization of sarcomeric actin filaments in chicken skeletal muscle cells
Saeko Miyauchi-Nomura, Takashi Obinata and Naruki Sato

Symmetric cell division of the mouse zygote requires an actin network
Ting Gang Chew, Chanchao Lorthongpanich, Wei Xia Ang, Barbara B. Knowles and Davor Solter

Regulation of late endosomal/lysosomal maturation and trafficking by cortactin affects Golgi morphology
Kellye C. Kirkbride, Nan Hyung Hong, Christi L. French, Emily S. Clark, W. Gray Jerome and Alissa M. Weaver

Structure and activity of full-length formin mDia1
Sankar Maiti, Alphee Michelot, Christopher Gould, Laurent Blanchoin, Olga Sokolova and Bruce L. Goode

Plus-end directed myosins accelerate actin filament sliding by single-headed myosin VI
Bhagavathi Ramamurthy, Wenxiang Cao, Enrique M. De La Cruz and Mark S. Mooseker

Diverse protective roles of the actin cytoskeleton during oxidative stress
Michelle E. Farah, Vladimir Sirotkin, Brian Haarer, David Kakhniashvili and David C. Amberg

WASH and the Arp2/3 complex regulate endosome shape and trafficking.
Steve N. Duleh, Matthew D. Welch

The structure of native G-actin.
Hui Wang, Robert C. Robinson, Leslie D. Burtnick

Isolation and partial purification of the Saccharomyces cerevisiae cytokinetic apparatus.
Brian A. Young, Christopher Buser, and David G. Drubin

A central role for the WH2 domain of Srv2/CAP in recharging actin monomers to drive actin turnover in vitro and in vivo.
Faisal Chaudhry, Kristin Little, Lou Talarico, Omar Quintero-Monzon, Bruce L. Goode

Delayed embryonic development and impaired cell growth and survival in Actg1 null mice.
Tina M. Bunnell, James M. Ervasti

GMF is an evolutionarily developed Adf/cofilin-super family protein involved in the Arp2/3 complex-mediated organization of the actin cytoskeleton.
Kentaro Nakano, Hidekazu Kuwayama, Masato Kawasaki, Osamu Numata, Masak Takaine

The formin FRL1 (FMNL1) is an essential component of macrophage podosomes
Akos T. Mersich, Matthew R. Miller, Halina Chkourko and Scott D. Blystone

Assembly of filopodia by the formin FRL2 (FMNL3)
Elizabeth S. Harris, Timothy J. Gauvin, Ernest G. Heimsath, Henry N. Higgs

Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules.
Jean M. Sanger, Jushuo Wang, Lisa M. Gleason, Prokash Chowrashi, Dipak K. Dube, Balraj Mittal, Victoria Zhukareva, and Joseph W. Sanger

The kinetics of force-induced cell reorganization depend on microtubules and actin.
Alexandra M. Goldyn, Peter Kaiser, Joachim P. Spatz, Christoph Ballestrem, Ralf Kemkemer