A novel l-isoleucine-4′-dioxygenase and l-isoleucine dihydroxylation cascade in Pantoea ananatis
Sergey V. Smirnov, Pavel M. Sokolov, Veronika A. Kotlyarova, Natalya N. Samsonova, Tomohiro Kodera, Masakazu Sugiyama, Takayoshi Torii, Makoto Hibi, Sakayu Shimizu, Kenzo Yokozeki and Jun Ogawa
Article first published online: 2 APR 2013 | DOI: 10.1002/mbo3.87
The genes encoding HilA and HilB from Pantoea ananatis AJ13355 were cloned and expressed in Escherichia coli. The culturing of E. coli cells expressing hilA (E. coli-HilA) or both hilA and hilB (E. coli-HilAB) in the presence of l-isoleucine resulted in the conversion of l-isoleucine into two novel biogenic compounds: l-4′-isoleucine and l-4,4′-dihydroxyisoleucine, respectively. In parallel, two novel enzymatic activities were detected in the crude cell lysates of the E. coli-HilA and E. coli-HilAB strains: l-isoleucine-4′-dioxygenase (HilA) and l-4′-hydroxyisoleucine-4-dioxygenase (HilB) activities, respectively.