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All articles accepted from 14 August 2012 are published under the terms of the Creative Commons Attribution License.  All articles accepted before this date, were published under a Creative Commons Attribution Non-Commercial License.

Cover image for Vol. 6 Issue 3

Edited by Pierre Cornelis, Microbial Interactions, VIB Department of Structural Biology, Vrije Universiteit Brussel, Brussels, Belgium

Impact Factor: 2.747

ISI Journal Citation Reports © Ranking: 2016: 56/124 (Microbiology)

Online ISSN: 2045-8827

Author Guidelines

MicrobiologyOpen – steps to publication

1. Submit or confirm your submission at
2. We will send you an email confirmation of your submission details.
3. After review and acceptance, you will be prompted to sign the Open Access Agreement form
. Payment of the article publication charge will be required. You can then track the progress of your article through Wiley Author Services.
4. You will receive notification that your proof is ready for review, and be able to make corrections to your article.
5. Your article will publish on Wiley Online Library. If you have previously signed up for alerts through Wiley’s Author Services, you will be sent an email when your article is published online.

You will be prompted to sign the Open Access Agreement electronically after manuscript review and acceptance. We have introduced a convenient new process for signing your Open Access Agreement electronically (eOAA) that will save you considerable time and effort. The author identified as the formal corresponding author for the paper will receive an email prompting them to login into Author Services; where via the Wiley Author Licensing Service (WALS) they will be able to complete the license agreement on behalf of all authors on the paper. You may preview the copyright terms and conditions here.

This journal now uses eLocators. For more information, please visit the Author Services eLocator page here.


Pierre Cornelis

Address correspondence to the Editorial Office:

The Journal requires that authors submit electronically via the online submission site at

Original articles
Review articles

Manuscripts must be submitted in grammatically correct English. Manuscripts that do not meet this standard cannot be reviewed. Authors for whom English is a second language may wish to consult an English-speaking colleague or consider having their manuscript professionally edited before submission to improve the English. A list of independent suppliers of editing services can be found at All services are paid for and arranged by the author, and use of one of these services does not guarantee acceptance or preference for publication. A manuscript is considered for review and possible publication on the condition that it is submitted solely to MicrobiologyOpen, and that the manuscript or a substantial portion of it is not under consideration elsewhere.

Dual Use Research
MicrobiologyOpen expects that all authors will conform to the National Science Advisory Board for Biosecurity (NSABB) guidelines for Dual Use Life Sciences Research ( For further information, and a description of ‘dual use research of concern’, please refer to the June 2007 NSABB report ( for transmittal 0807_Sept07.pdf). If any of the reported studies may fall in any of these categories, the Editor-in-Chief must be informed at the time of manuscript submission.

Distribution of Strains and Materials
The publication of an article in MicrobiologyOpen is subject to the understanding that authors will distribute freely any strains, clones or antibodies described therein for use in academic research. Authors might wish to make their plasmid constructs available free of charge through Addgene (

Informed Consent
MicrobiologyOpen requires that all appropriate steps be taken in obtaining informed consent of any and all human and/or experimental animal subjects participating in the research comprising the manuscript submitted for review and possible publication. A statement indicating that the protocol and procedures employed were reviewed and approved by the appropriate institutional review committee must be included in the Methods section of the manuscript. For research involving recombinant DNA, containment facilities and guidelines should conform to those of the National Institutes of Health or corresponding institutions. For those investigators who do not have formal ethics review committees, the principles outlined in the Helsinki Declaration should be followed.

Disclosure Statement
MicrobiologyOpen requires that all authors disclose any potential sources of conflict of interest. Any interest or relationship, financial or otherwise, that might be perceived as influencing an author’s objectivity is considered a potential source of conflict of interest. These must be disclosed when directly relevant or directly related to the work that the authors describe in their manuscript. Potential sources of conflict of interest include, but are not limited to, patent or stock ownership, membership of a company board of directors, membership of an advisory board or committee for a company, and consultancy for or receipt of speaker’s fees from a company. The existence of a conflict of interest does not preclude publication in this journal.

If the authors have no conflict of interest to declare, they must also state this at submission.

It is the responsibility of the corresponding author to review this policy with all authors and collectively to list on the cover letter to the Editor-in-Chief, in the manuscript (under the Acknowledgements section), and in the online submission system ALL pertinent commercial and other relationships.

Manuscript Preparation
We place very few restrictions on the way in which you prepare your article, and it is not necessary to try to replicate the layout of the journal in your submission. We ask only that you consider your reviewers by supplying your manuscript in a clear, generic and readable layout, and ensure that all relevant sections are included. Our production process will take care of all aspects of formatting and style. The list below can be used as a checklist to ensure that the manuscript has all the information necessary for successful publication.

     Title page, including title, authors’ names, authors’ affiliations, and contact details (especially email address) for the person to whom the proof notification is to be sent.
     Abstract and 4–6 keywords
     Text (introduction, materials and methods, results, discussion)
     Literature cited (see below for tips on references)
     Tables (may be sent as a separate file if necessary)
     Figure legends
     Acknowledgements, including details of funding bodies with grant numbers

Genetic Nomenclature
Standard genetic nomenclature should be used. For further information, including relevant websites, authors should refer to the Genetic Nomenclature Guide in Trends in Genetics (Elsevier Science Ltd, 1995). For other detailed information, authors should consult Bachman (Microbiol Rev 47: 180–230, 1983) for E. coli K-12, Sanderson and Roth (Microbiol Rev 47: 310–453, 1983) for Salmonella typhimurium; ,Holloway et al. (Microbiol Rev 43: 73–102, 1979) for Bacillus subtilis; Perkins et al. (Microbiol Rev 46: 426–570, 1982) for Neurospora crassa; and the Handbook of Genetics Vol. 1 (R. C. King, ed., Plenum Press, 1974) for Saccharomyces cerevisiae. Nomenclature for DNA restriction and modification enzymes and their genes should follow Roberts et al. (Nucleic Acids Res 31: 1805-1812, 2003).

As with the main body of text, the completeness and content of your reference list is more important than the format chosen. A clear and consistent, generic style will assist the accuracy of our production processes and produce the highest quality published work, but it is not necessary to try to replicate the journal’s own style, which is applied during the production process. If you use bibliographic software to generate your reference list, select a standard output style, and check that it produces full and comprehensive reference listings. A guide to the minimum elements required for successful reference linking appears below. The final journal output will use the ‘Harvard’ style of reference citation. If your manuscript has already been prepared using the ‘Vancouver’ system, we are quite happy to receive it in this form. We will perform the conversion from one system to the other during the production process.

Minimum Reference Information

Journal Article
Author(s) in full
Year of publication
Article title
Journal title (preferably not abbreviated)
Volume number
Issue number
Page range

Author(s) in full
Year of publication
Book title
Place of publication
No. Pages

Book Chapter
Author(s) in full
Year of publication
Chapter title
Book Author/Editor
Book title
Place of publication
Page range

Online Resources
References to online research articles should always include a DOI, where available. When referring to other Web pages, it is useful to include a date on which the resource was accessed.

All tables must be cited in the text in the order that they should appear.

All figures must be cited in the text in the order that they should appear. Illustrations are an important medium through which to convey the meaning in your article, and there is no substitute for preparing these to the highest possible standard. Therefore, please create your illustrations carefully with reference to our graphics guidelines (see It is very difficult to improve an image that has been saved or created in an inappropriate format. We realize that not everyone has access to high-end graphics software, so the following information may help if you are having difficulty in deciding how to get the best out of the tools at your disposal.

1. Check your software options to see if you can ‘save as’ or ‘export’ using one of the robust, industry-standard formats. These are:·         Encapsulated PostScript (EPS)
·         Tagged Image File Format (TIFF)
·         Portable Network Graphics (PNG)
·         Portable Document Format (PDF)

2. As a general rule of thumb, images that contain text and line art (graphs, charts, maps, etc.) will reproduce best if saved as EPS or PDF. If you choose this option, it is important to remember to embed fonts. This ensures that any text reproduces exactly as you intend.

3. Images that contain photographic information are best saved as TIFF or PNG, as this ensures that all data are included in the file. JPEG (Joint Photographic Experts Group) should be avoided if possible, as information is lost during compression; however, it is acceptable for purely photographic subjects if the image was generated as a JPEG from the outset (many digital cameras, for example, output only in JPEG format).

4. If you are not sure which format would be the best option, it is always best to default to EPS or PDF as these are more likely to preserve the high-quality characteristics of the original.

5. Microsoft Office. If you have generated your images in Microsoft Office software (Word, Excel, PowerPoint), or similar, it is often best simply to send us the files in their native file formats.

6. Please ensure all images are a minimum of 600 dpi.

Metric System
The metric system should be used for all measurements, weights, etc. Temperatures should be expressed in degrees Celsius (centigrade).

Supporting Information and Data Deposition

Supporting Information can be a useful way for an author to include important but ancillary information with the online version of an article. Examples of Supporting Information include additional tables, data sets, figures, movie files, audio clips, 3D structures, and other related nonessential multimedia files. Supporting Information should be cited within the article text, and a descriptive legend should be included. It is published as supplied by the author, and a proof is not made available prior to publication; for these reasons, authors should provide any Supporting Information in the desired final format.

For further information on recommended file types and requirements for submission, please visit:

It is the policy of MicrobiologyOpen that sequence data must be deposited in the EMBL/GenBank/DDBJ Nucleotide Sequence Data Libraries, the accession number cross-referenced in the published manuscript, and the data made fully available at the time of publication. It is only necessary to submit to one database. The suggested wording for referring to accession-number information is: ‘These sequence data have been submitted to the DDBJ/eMBL/ GenBank databases under accession number U12345’. Details of data submission can be found at: DDBJ/DNA Data Bank of Japan: EMBL: GenBank:

Microarray datasets should be presented in compliance with current practices (see, for example, (MGeD Society at and deposited in an accredited data base such as Array express ( or GEO ( The corresponding reference to the database entry should be included in the text.

Search Engine Optimization for Your Paper

Please consult our SEO Tips for Authors page in order to maximize online discoverability for your published research. Included are tips for making your title and abstract SEO-friendly, choosing appropriate keywords, and promoting your research through social media.

Cover Images
MicrobiologyOpen encourages you to propose one of the figures in your paper as a possible online journal cover, and for potential publication on the official blog of Wiley Open Access:

CrossCheck is a multi-publisher initiative to screen published and submitted content using iThenticate. To find out more about CrossCheck visit By submitting your manuscript to MicrobiologyOpen you accept that your manuscript may be screened, using the iThenticate tool, for textual similarity to other previously published works.

Soon after acceptance, you will receive an email containing your PDF proof. Once you have submitted your corrections, the production office will finalize the layout of your article for publication.

As this is an open access journal, you have free, unlimited access to your article online. However, if you wish to obtain printed reprints, these may be ordered online: (email:

Production Questions
Please direct any questions regarding the production of your article to the Production Editor at