Humoral responses to HIVconsv induced by heterologous vaccine modalities in rhesus macaques
Nicola J. Borthwick, Maximillian Rosario, Torben Schiffner, Emma Bowles, Tina Ahmed, Peter Liljeström, Guillaume E. Stewart-Jones, Jan W. Drijfhout, Cornelis J. M. Melief and Tomáš Hanke
Article first published online: 11 MAR 2015 | DOI: 10.1002/iid3.52
The humoral immune response was evaluated in macaques immunised with a vaccine composed of the most conserved regions of HIV-1 and delivered by plasmid DNA, human adenovirus serotype 5, simian adenovirus serotype 63, MVA, Semliki Forest virus replicons and adjuvanted overlapping synthetic long peptides (SLP). We found that only the SLP formulation, but none of the genetic vaccine platforms induced antibodies recognizing linear HIVconsv epitopes, median 32/46 SLP.HIVconsv peptides. These antibodies bound to 15-mer and SLP peptides, recombinant gp120 and trimeric gp140 of HIV-1 Bal, YU2, JRFL and UG037, but failed to react with HIV-1 Bal and IIIB virions and HIV-1 Bal- and IIIB-infected human cells, and consequently failed to induce neutralizing antibodies. Antibodies induced by the SLP.HIVconsv vaccination resulted in positive signals in routine HIV-1 tests, an observation that provides important guidance for recruiting volunteers into future clinical trials.