Advanced Healthcare Materials

Cover image for Vol. 4 Issue 16

Early View (Online Version of Record published before inclusion in an issue)


  1. 1 - 42
  1. Full Papers

    1. Multiplexed Dosing Assays by Digitally Definable Hydrogel Volumes

      Adele Faralli, Fredrik Melander, Esben Kjær Unmack Larsen, Sergey Chernyy, Thomas L. Andresen and Niels B. Larsen

      Article first published online: 30 NOV 2015 | DOI: 10.1002/adhm.201500542

      Thumbnail image of graphical abstract

      Embedding of free or liposome-encapsulated active compounds in PEG–diacrylate hydrogels of sizes defined by digital light projection is a scalable and flexible method for multiplexed definition of combination drug dosing. Hydrogel material optimization allows dosing of two first-line chemotherapeutic combination regimens for colorectal cancer, as demonstrated on a colorectal cancer cell line.

    2. Liposome-Loaded Cell Backpacks

      Roberta Polak, Rosanna M. Lim, Marisa M. Beppu, Ronaldo N. M. Pitombo, Robert E. Cohen and Michael F. Rubner

      Article first published online: 30 NOV 2015 | DOI: 10.1002/adhm.201500604

      Thumbnail image of graphical abstract

      Liposomes loaded with doxorubicin are successfully embedded into cell backpacks with retention of their structure. Liposome-loaded backpacks are attached to the surface of living monocytes, which remain viable over timescales relevant to usual drug delivery schemes. One new interesting observation suggests a potential new use of cell backpacks as vectors for the direct targeting of cancer cells.

  2. Communications

    1. LumeNEXT: A Practical Method to Pattern Luminal Structures in ECM Gels

      José A. Jiménez-Torres, Stephen L. Peery, Kyung E. Sung and David J. Beebe

      Article first published online: 26 NOV 2015 | DOI: 10.1002/adhm.201500608

      Thumbnail image of graphical abstract

      In vitro biomimetic modeling of physio­logical structures bridges the gap between 2D in vitro culture and animal models. Lumens (tubular structures) are ubiquitous in vivo, being present in blood vessels, mammary ducts, and the lymphatic system. A method ‘LumeNEXT' is presented here that allows the fabrication of 3D embedded lumens where size, structure, distance, and configuration can be controlled using standard poly­dimethylsiloxane micromolding methods.

  3. Full Papers

    1. Biological and Tribological Assessment of Poly(Ethylene Oxide Terephthalate)/Poly(Butylene Terephthalate), Polycaprolactone, and Poly (L\DL) Lactic Acid Plotted Scaffolds for Skeletal Tissue Regeneration

      Wilhelmus J. Hendrikson, Xiangqiong Zeng, Jeroen Rouwkema, Clemens A. van Blitterswijk, Emile van der Heide and Lorenzo Moroni

      Article first published online: 25 NOV 2015 | DOI: 10.1002/adhm.201500067

      Thumbnail image of graphical abstract

      Poly(caprolactone), poly(L/DL)lactic-acid and poly(ethylene oxide terephthalate)/poly(butylene terephthalate) scaffolds are tested for their biological and tribological behavior. The biomaterials are compared biologically by exposing stem-cell-seeded scaffolds to differentiation media. The effects of biomaterial and scaffold architecture on friction are assessed. The best performing biomaterial and architecture are combined to investigate the influence of extracellular matrix formation on friction coefficient.

    2. Sensing and Sensibility: Single-Islet-based Quality Control Assay of Cryopreserved Pancreatic Islets with Functionalized Hydrogel Microcapsules

      Wanyu Chen, Zhiquan Shu, Dayong Gao and Amy Q. Shen

      Article first published online: 25 NOV 2015 | DOI: 10.1002/adhm.201500515

      Thumbnail image of graphical abstract

      The single-islet-based quality control assay consists of a droplet microfluidic device for islet encapsulation with alginate hydrogel, with oxygen sensitive dye embedded in the capsules for quality control, with an optimized cryopreservation protocol.

    3. Exploring the Potential of Starch/Polycaprolactone Aligned Magnetic Responsive Scaffolds for Tendon Regeneration

      Ana I. Gonçalves, Márcia T. Rodrigues, Pedro P. Carvalho, Manuel Bañobre-López, Elvira Paz, Paulo Freitas and Manuela E. Gomes

      Article first published online: 25 NOV 2015 | DOI: 10.1002/adhm.201500623

      Thumbnail image of graphical abstract

      3D rapid prototyped scaffolds with aligned architecture and magnetic responsiveness promote tenogenic differentiation of adipose stem cells with increasing ECM deposition stimulated by an externally applied magnetic field. The magnetic scaffolds also show biocompatibility in an ectopic rat model. These findings guide the rational design of magnetically responsive scaffolds mimicking tendon microenvironment and enabling the combination of therapeutic and diagnostic tools.

    4. Gallium-Loaded Dissolvable Microfilm Constructs that Provide Sustained Release of Ga3+ for Management of Biofilms

      Maggie Herron, Michael J. Schurr, Christopher J. Murphy, Jonathan F. McAnulty, Charles J. Czuprynski and Nicholas L. Abbott

      Article first published online: 24 NOV 2015 | DOI: 10.1002/adhm.201500599

      Thumbnail image of graphical abstract

      Bacterial biofilms have been shown to delay the healing of wounds. Here, the incorporation of the antibiofilm agent Ga3+ into polymeric nanofilms, modification of the nanofilms by cross-linking to control the release rate of Ga3+, integration of the nanofilms onto wound dressings using micrometer-thick water-soluble casts, and the effectiveness of the nanofilms in inhibiting and dispersing biofilms are reported.

  4. Communications

    1. An Apoferritin-based Drug Delivery System for the Tyrosine Kinase Inhibitor Gefitinib

      Anchala I. Kuruppu, Lei Zhang, Hilary Collins, Lyudmila Turyanska, Neil R. Thomas and Tracey D. Bradshaw

      Article first published online: 23 NOV 2015 | DOI: 10.1002/adhm.201500389

      Thumbnail image of graphical abstract

      Anticancer drug Gefitinib encapsulated within human heavy chain apoferritin by diffusion allows pH-controlled sustained release of cargo. The combination of increased cellular uptake, and potent and enhanced antitumor activity against the HER2 overexpressing SKBR3 cell line compared to Gefitinib alone, makes it a promising carrier for delivery of drugs to tumor sites.

  5. Reviews

    1. Bridging the Gap: From 2D Cell Culture to 3D Microengineered Extracellular Matrices

      Yanfen Li and Kristopher A. Kilian

      Article first published online: 23 NOV 2015 | DOI: 10.1002/adhm.201500427

      Thumbnail image of graphical abstract

      Mammalian cells have historically been cultured in the laboratory on rigid, planar substrates. However, cell signaling in 2D environments often fails to recapitulate the complex dynamic processes that occur in vivo. Here we explore how the field of engineered extracellular matrices has evolved from 2D cell culture to 3D microengineered systems that better reflect the complexity of native tissue.

  6. Full Papers

    1. Rapid Optical Cavity PCR

      Jun Ho Son, SoonGweon Hong, Amanda J. Haack, Lars Gustafson, Minsun Song, Ori Hoxha and Luke P. Lee

      Article first published online: 23 NOV 2015 | DOI: 10.1002/adhm.201500708

      Thumbnail image of graphical abstract

      Novel optical cavity polymerase chain reaction (PCR) driven by a light-emitting diode (LED) is demonstrated for fast, accurate, and reliable PCR-based diagnostics. Using optical cavity PCR, nucleic acid amplification with the template DNA concentration has been successfully demonstrated, ranging from 10−5 to 10−8 ng μL−1 within 5–15 min with excellent repeatability and reproducibility.

    2. Preparation of Engineered Salmonella Typhimurium-Driven Hyaluronic-Acid-Based Microbeads with Both Chemotactic and Biological Targeting Towards Breast Cancer Cells for Enhanced Anticancer Therapy

      Saji Uthaman, Shaohui Zheng, Jiwon Han, Young Jin Choi, Sunghoon Cho, Van Du Nguyen, Jong-Oh Park, Seung-Hwan Park, Jung-Joon Min, Sukho Park and In-Kyu Park

      Article first published online: 19 NOV 2015 | DOI: 10.1002/adhm.201500556

      Thumbnail image of graphical abstract

      Novel dual-tumor-targeting bacteriobots are developed by the integration of CD44-targeted hyaluronic acid microbeads and the tumor-targeting attenuated bacteria strain, Salmonella Typhimurium. These bacteriobots self-propel, and, upon reaching a tumor region, deliver therapeutic cargo embedded inside the microbeads. The combination of a biological system with micro- and nanotechnology is a promising approach towards the development of bio-hybrid microrobots with interesting potential applications.

    3. Bioactive Glass for Large Bone Repair

      Weitao Jia, Grace Y. Lau, Wenhai Huang, Changqing Zhang, Antoni P. Tomsia and Qiang Fu

      Article first published online: 19 NOV 2015 | DOI: 10.1002/adhm.201500447

      Thumbnail image of graphical abstract

      Bioactive glass scaffolds (top right) are demonstrated to be able to bridge large segmental bone defect (left) in a rabbit femur model. Active new bone and blood vessel formation are observed throughout scaffolds (bottom right). It is shown that, for the first time, a synthetic material alone can repair large bone defects as efficiently as the autologous bone graft.

  7. Reviews

    1. Supported Lipid Bilayers for the Generation of Dynamic Cell–Material Interfaces

      Jasper van Weerd, Marcel Karperien and Pascal Jonkheijm

      Article first published online: 17 NOV 2015 | DOI: 10.1002/adhm.201500398

      Thumbnail image of graphical abstract

      Supported lipid bilayers (SLBs) have great potential as a biomimetic cell interface. Their use as a cell membrane mimic has evolved into a platform for bioanalysis and the study of cell–cell and cell–extracellular matrix interactions. The fabrication, modification, analysis, in vitro and in vivo applications of SLBs are reviewed.

  8. Full Papers

    1. Are Fluorescent Organic Nanoparticles Relevant Tools for Tracking Cancer Cells or Macrophages?

      Adrien Faucon, Houda Benhelli-Mokrani, Luis A. Córdova, Bénédicte Brulin, Dominique Heymann, Philippe Hulin, Steven Nedellec and Eléna Ishow

      Article first published online: 9 NOV 2015 | DOI: 10.1002/adhm.201500562

      Thumbnail image of graphical abstract

      Long-term fate of fluorescent organic nanoparticles (FONs), known as very bright imaging agents and made of self-assembled solvatochromic fluorophores, is explored in both cancer cells and monocytes/macrophages. Disintegration of the orange-emitting FONs by monocytes/macrophages is evidenced through the formation of micrometer green-yellowish emitting vesicles. By contrast, cancer cells retain longer the integrity of organic nanoparticles.

  9. Communications

    1. Discovering of Tumor-targeting Peptides using Bi-functional Microarray

      Weizhi Wang, Zihua Wang, Xiangli Bu, Ren Li, Mingxing Zhou and Zhiyuan Hu

      Article first published online: 9 NOV 2015 | DOI: 10.1002/adhm.201500724

      Thumbnail image of graphical abstract

      A bi-functional microarray for in situ peptide screening is presented herein, from which an affinity peptide towards EpCAM was screened out for tumor cell capture.

  10. Full Papers

    1. Bioinspired Oil Core/Silica Shell Nanocarriers with Tunable and Multimodal Functionalities

      Anshuman Jakhmola, Raffaele Vecchione, Daniela Guarnieri, Valentina Belli, Dominic Calabria and Paolo A. Netti

      Article first published online: 29 OCT 2015 | DOI: 10.1002/adhm.201500588

      Thumbnail image of graphical abstract

      A novel multilayered and monodisperse nanocarrier system with tunable and multi­modal functionalities is designed. This new system is prepared at ambient conditions and features multiple platforms for loading two or more different molecules simultaneously. The poly-L-lysine layer controls the deposition of outermost silica layer and also modulates its charge from positive to negative by a simple strategy.

    2. Dermal Patch with Integrated Flexible Heater for on Demand Drug Delivery

      Sara Bagherifard, Ali Tamayol, Pooria Mostafalu, Mohsen Akbari, Mattia Comotto, Nasim Annabi, Masoumeh Ghaderi, Sameer Sonkusale, Mehmet R. Dokmeci and Ali Khademhosseini

      Article first published online: 26 OCT 2015 | DOI: 10.1002/adhm.201500357

      Thumbnail image of graphical abstract

      A wearable drug delivery system based on a hydrogel sheet containing thermo­responsive drug carriers interfaced with a flexible heater is engineered for topical applications. The platform maintains a conformal contact with skin and enables on demand and tunable drug delivery by controlling the hydrogel temperature. The release rates of various molecules with different sizes are studied. The system paves the way toward engineering smart wound dressings.

    3. Investigation of the Viability, Adhesion, and Migration of Human Fibroblasts in a Hyaluronic Acid/Gelatin Microgel-Reinforced Composite Hydrogel for Vocal Fold Tissue Regeneration

      Hossein K. Heris, Jamal Daoud, Sara Sheibani, Hojatollah Vali, Maryam Tabrizian and Luc Mongeau

      Article first published online: 26 OCT 2015 | DOI: 10.1002/adhm.201500370

      Thumbnail image of graphical abstract

      Cell adhesion integrins and kinematics of cell motion are investigated in a novel hyaluronic acid (HA)–gelatin (Ge) microgel-reinforced composite hydrogel (MRCH) scaffold. Human fibroblast cells establish effective adhesion to HA–Ge microgels through the ubiquitous expression of β1 integrin in the cell membrane. The cells within the MRCH have an average motility speed of 0.24 ± 0.08 μm min−1.

    4. Nanoparticle-Mediated Target Delivery of TRAIL as Gene Therapy for Glioblastoma

      Kui Wang, Forrest M. Kievit, Mike Jeon, John R. Silber, Richard G. Ellenbogen and Miqin Zhang

      Article first published online: 26 OCT 2015 | DOI: 10.1002/adhm.201500563

      Thumbnail image of graphical abstract

      A chitosan–PEG-grafted polyethyleneimine (PEI) copolymer-coated iron oxide nanoparticle (NP) can effectively deliver TRAIL encoding plasmid DNA into in glioblastoma (GBM) cells with no notable cytotoxicity demonstrated in vitro. In vivo systemic administration of chlorotoxin (CTX)-activated NP–TRAIL complexes in mice bearing GBM xenografts induces apoptosis of tumor cells and a sustainable reduction of tumor burden.

    5. Cellular and Mitochondrial Dual-Targeted Organic Dots with Aggregation-Induced Emission Characteristics for Image-Guided Photodynamic Therapy

      Guangxue Feng, Wei Qin, Qinglian Hu, Ben Zhong Tang and Bin Liu

      Article first published online: 19 OCT 2015 | DOI: 10.1002/adhm.201500431

      Thumbnail image of graphical abstract

      Dual-targeted nanoparticles (NPs) are fabricated by nanoprecipitation method. Bearing the aggregation-induced emission characteristics (AIE), the obtained AIE NPs show bright red fluorescence and efficient reactive oxygen species' production. The NPs are able to selectively internalize into cancer cells with folate receptor overexpression, and subsequently accumulate at mitochondria, which largely improve the photodynamic therapy efficiency.

    6. Injectable, Pore-Forming Hydrogels for In Vivo Enrichment of Immature Dendritic Cells

      Catia S. Verbeke and David J. Mooney

      Article first published online: 16 OCT 2015 | DOI: 10.1002/adhm.201500618

      Thumbnail image of graphical abstract

      An injectable material system that enriches immature dendritic cells (DCs) locally in vivo is described, which is of interest for the development of biomaterials-based immunotherapies. Noninflammatory, pore-forming hydrogels are combined with gold nanoparticles (AuNPs) for controlled delivery of a DC recruitment factor, leading to effective DC enrichment. Following subcutaneous injection, several million immature DCs accumulate within the gels in vivo.

    7. Design and Characterization of a Multifunctional pH-Triggered Peptide C8 for Selective Anticancer Activity

      Sheng Lu, W. F. Drew Bennett, Yong Ding, Lei Zhang, Helen Y. Fan, Danyang Zhao, Tao Zheng, Ping-Kai Ouyang, Jason Li, Yan Wu, Wen Xu, Dafeng Chu, Yongfang Yuan, Heiko Heerklotz, Mikko Karttunen and P. Chen

      Article first published online: 16 OCT 2015 | DOI: 10.1002/adhm.201500636

      Thumbnail image of graphical abstract

      A multifunctional cationic lytic peptide is designed incorporating amino acid pairing, amphipathic structure, and histidine-mediated pH-sensitivity for controllable membrane lytic activity and nanostructure. The peptide can deliver small-molecule drugs in a pH-triggered manner with selectively enhanced synergetic anticancer activity, which originates from the selective lytic activity of the peptide, representing a novel therapeutic carrier strategy.

    8. Hierarchical Nanofibrous Microspheres with Controlled Growth Factor Delivery for Bone Regeneration

      Chi Ma, Yan Jing, Hongchen Sun and Xiaohua Liu

      Article first published online: 13 OCT 2015 | DOI: 10.1002/adhm.201500531

      Thumbnail image of graphical abstract

      A unique hierarchical nanosphere-encapsulated-in-microsphere scaffolding system is developed as a new injectable cell carrier. Heparin-binding growth factors are encapsulated in gelatin nanospheres and further entrapped by nanofibrous microspheres. Therefore, this system allows the integration of biomimetic materials design with controlled growth factor delivery into one injectable microsphere, leading to enhanced tissue regeneration.

  11. Communications

    1. Carbohydrate-Conjugated Hollow Oblate Mesoporous Silica Nanoparticles as Nanoantibiotics to Target Mycobacteria

      Nanjing Hao, Xuan Chen, Seaho Jeon and Mingdi Yan

      Article first published online: 9 OCT 2015 | DOI: 10.1002/adhm.201500491

      Thumbnail image of graphical abstract

      Engineering nanomaterials with enhanced antibacterial activities remains a critical and practical challenge. Hollow oblate mesoporous silica nanoparticles (HOMSNs) are synthesized by a simple protocol of ammonia hydrothermal treatment of oblate mesoporous silica nanoparticles prepared using dibenzyl ether as a cosolvent. When conjugated with trehalose as the targeting ligand, the antibiotic-encapsulated HOMSNs exhibit high binding affinity and antibacterial efficacy toward mycobacteria.

    2. Nuclear-Targeting MSNs-Based Drug Delivery System: Global Gene Expression Analysis on the MDR-Overcoming Mechanisms

      Xiaoyu Li, Limin Pan and Jianlin Shi

      Article first published online: 9 OCT 2015 | DOI: 10.1002/adhm.201500548

      Thumbnail image of graphical abstract

      The biological mechanisms of nuclear-targeting mesoporous silica nanoparticles (MSNs)-based DDSs (DOX@NT-MSNs) in overcoming multidrug resistance of cancer cells have been studied. It is interesting to find for the first time that DOX@NT-MSNs down-regulate the expression of apoptosis suppressor genes and inhibit DNA repair process by disturbing the p53 pathway.

  12. Full Papers

    1. Photomediated Reactive Oxygen Species-Generable Nanoparticles for Triggered Release and Endo/Lysosomal Escape of Drug upon Attenuated Single Light Irradiation

      Eun Ha Seo, Chung-Sung Lee and Kun Na

      Article first published online: 9 OCT 2015 | DOI: 10.1002/adhm.201500622

      Thumbnail image of graphical abstract

      Photomediated reactive oxygen species (ROS)-generable nanoparticles (SRNs) incorporated with ROS-generator and scavenger in a single delivery platform is developed. Under attenuated single light irradiation, doxorubicin loaded SRNs (D-SRNs) synergistically exhibit simultaneous triggered drug release and endo/lysosomal escape as well as photodynamic therapy for highly efficient cancer treatment.

  13. Reviews

    1. Microneedles for Transdermal Biosensing: Current Picture and Future Direction

      Letizia Ventrelli, Lucanos Marsilio Strambini and Giuseppe Barillaro

      Article first published online: 6 OCT 2015 | DOI: 10.1002/adhm.201500450

      Thumbnail image of graphical abstract

      Microneedles, a microscale replica of hypodermic needles, represent a new frontier of transdermal biosensing for analytes of clinical interest, e.g., glucose, biomarkers, and ions. The ultimate goal is microneedle-based pain-free and easy-to-use miniaturized biochips for point-of-care analysis, either in situ or ex situ, in transdermal bodily fluids. The current state and future direction of this novel, important, and fascinating research trend is analyzed.

  14. Full Papers

    1. Cubical Shape Enhances the Interaction of Layer-by-Layer Polymeric Particles with Breast Cancer Cells

      Jenolyn F. Alexander, Veronika Kozlovskaya, Jun Chen, Thomas Kuncewicz, Eugenia Kharlampieva and Biana Godin

      Article first published online: 1 OCT 2015 | DOI: 10.1002/adhm.201500537

      Thumbnail image of graphical abstract

      Understanding the role of shape and elasticity in the behavior of objects in the tumor microenvironment is crucial for designing more efficient and less toxic therapies. Sphere- and cube-shaped biocompatible elastic microshells and their rigid core-shell precursors were explored. Cubical particles interact better with breast cancer cells, while elasticity prevents engulfment by the cells of reticulo–endothelial system.

    2. Functionalization of a Collagen–Hydroxyapatite Scaffold with Osteostatin to Facilitate Enhanced Bone Regeneration

      Elaine Quinlan, Emmet M. Thompson, Amos Matsiko, Fergal J. O'Brien and Adolfo López-Noriega

      Article first published online: 28 SEP 2015 | DOI: 10.1002/adhm.201500439

      Thumbnail image of graphical abstract

      Bone tissue regeneration can be accelerated by the use of tissue-engineered scaffolds delivering therapeutic peptides. Chemically immobilizing osteostatin within a collagen–hydroxyapatite scaffold via a crosslinking method supports the release of 4% of the peptide after 21 d. This scaffold demonstrates an osteogenic effect when seeded with preosteoblasts and significantly enhances bone regeneration when implanted in a rat calvarial defect.

    3. Engineering-Aligned 3D Neural Circuit in Microfluidic Device

      Seokyoung Bang, Sangcheol Na, Jae Myung Jang, Jinhyun Kim and Noo Li Jeon

      Article first published online: 2 SEP 2015 | DOI: 10.1002/adhm.201500397

      Thumbnail image of graphical abstract

      A novel microfluidic platform for formation of 3D neural circuit is reported. Microfluidic channels are filled with Matrigel and hydrostatic pressure is maintained across the width of the gel during cross-linking. This generates aligned extracellular matrix (ECM) structures in the Matrigel to guide the axon growth direction. Compared to neurons cultured in randomly cross-linked Matrigel, axon bundles that connect pre-/post-synaptic neurons can be formed.

  15. Communications

    1. Dual Release Carriers for Cochlear Delivery

      Sahar Rahmani, Astin M. Ross, Tae-Hong Park, Hakan Durmaz, Acacia F. Dishman, Diane M. Prieskorn, Nathan Jones, Richard A. Altschuler and Joerg Lahann

      Article first published online: 14 JUL 2015 | DOI: 10.1002/adhm.201500141

      Thumbnail image of graphical abstract

      The fabrication of a carrier system capable of selectively encapsulating two therapeutics, piribedil and glial cell derived neurotrophic factor, in select compartments, and the programming of their independent release rates is investigated. The characterization of these particles, the release of each therapeutic, and the persistence of the particles within Guinea pig cochlea are assessed.

  16. Full Papers

    1. Patterning of Fibroblast and Matrix Anisotropy within 3D Confinement is Driven by the Cytoskeleton

      Janna V. Serbo, Scot Kuo, Shawna Lewis, Matthew Lehmann, Jiuru Li, David H. Gracias and Lewis H. Romer

      Article first published online: 1 JUN 2015 | DOI: 10.1002/adhm.201500030

      Thumbnail image of graphical abstract

      A platform is built to engineer the dimensions and aspect ratios of cell-derived matrix scaffolds for the study of microtissue alignment—an important design feature for tissue engineering. Actin, myosin, and microtubules are found to be drivers of cell and matrix responses to 3D confinement.

  17. Communications

    1. M13 Bacteriophage and Adeno-Associated Virus Hybrid for Novel Tissue Engineering Material with Gene Delivery Functions

      So Young Yoo, Hyo-Eon Jin, Dong Shin Choi, Masae Kobayashi, Yohan Farouz, Sky Wang and Seung-Wuk Lee

      Article first published online: 22 MAY 2015 | DOI: 10.1002/adhm.201500179

      Thumbnail image of graphical abstract

      A novel hybrid phage carrying genes from prokaryotic M13 phage and eukaryotic adeno-associated viruses can be used as a tissue engineering material with gene delivery functions. The filamentous shape of the resulting hybrid phage easily forms nanofibrous matrices, which can support cellular growth in tissue culture conditions and deliver the target programmed gene information into the target cells.

    2. Cephalopod-Inspired Miniaturized Suction Cups for Smart Medical Skin

      Moon Kee Choi, Ok Kyu Park, Changsoon Choi, Shutao Qiao, Roozbeh Ghaffari, Jaemin Kim, Dong Jun Lee, Myungbin Kim, Wonji Hyun, Seok Joo Kim, Hye Jin Hwang, Seung-Hae Kwon, Taeghwan Hyeon, Nanshu Lu and Dae-Hyeong Kim

      Article first published online: 20 MAY 2015 | DOI: 10.1002/adhm.201500285

      Thumbnail image of graphical abstract

      Biomimetic miniaturized suction cups (mSCs) are designed for the patient friendly, dry adhesives of smart medical skin. Both strong van der Waals force and induced negative pressure by the ultrasoft mSCs facilitate tight skin coupling without discomfort or irritations, improve sensitivities of the embedded stretchable electronics for continuous vital sign monitoring, and enable multiple drug reloading without loss of the adhesion.

  18. Full Papers

    1. Electroconductive Nanopatterned Substrates for Enhanced Myogenic Differentiation and Maturation

      Hee Seok Yang, Bora Lee, Jonathan H. Tsui, Jesse Macadangdang, Seok-Young Jang, Sung Gap Im and Deok-Ho Kim

      Article first published online: 18 MAY 2015 | DOI: 10.1002/adhm.201500003

      Thumbnail image of graphical abstract

      Bioinspired cell culture substrates are developed to enhance myogenic differentiation and maturation. Polyurethane acrylate films nanopatterned using capillary force lithography are coated with a thin layer of titanium or gold with electron beam deposition to create electroconductive substrates with well-defined nanotopography. These not only induce increased myoblast alignment, but also promote formation of myotubes expressing sarcomeric myosin heavy chain.

    2. In Vitro and In Vivo Evaluation of Whitlockite Biocompatibility: Comparative Study with Hydroxyapatite and β-Tricalcium Phosphate

      Hae Lin Jang, Guang Bin Zheng, Jungha Park, Hwan D. Kim, Hae-Ri Baek, Hye Kyoung Lee, Keunho Lee, Heung Nam Han, Choon-Ki Lee, Nathaniel S. Hwang, Jae Hyup Lee and Ki Tae Nam

      Article first published online: 12 MAY 2015 | DOI: 10.1002/adhm.201400824

      Thumbnail image of graphical abstract

      Inspired by human hard tissue, whitlockite (Ca18Mg2(HPO4)2(PO4)12) nanoparticles are synthesized and evaluated through in vitro and in vivo studies. As a cellular scaffold, whitlockite enhances the osteoblastic differentiation of mesenchymal stem cells better than hydroxyapatite. In addition, whitlockite implants successfully induce bone regeneration in a calvarial defect of rat model, similar to hydroxyapatite and β-tricalcium phosphate.

    3. Theoretical and Experimental Studies of Epidermal Heat Flux Sensors for Measurements of Core Body Temperature

      Yihui Zhang, Richard Chad Webb, Hongying Luo, Yeguang Xue, Jonas Kurniawan, Nam Heon Cho, Siddharth Krishnan, Yuhang Li, Yonggang Huang and John A. Rogers

      Article first published online: 7 MAY 2015 | DOI: 10.1002/adhm.201500110

      Thumbnail image of graphical abstract

      Theoretical approaches, modeling algorithms, materials, and device designs are introduced for noninvasive measurement of core body temperature by using multiple differential temperature sensors that attach intimately onto skin surfaces. Systematic studies of sensitivity, accuracy, and response time include results from analytical models, numerical calculations, and experimental measurement. These findings have potential use in long-term, continuous monitoring of core body temperature.

  19. Reviews

    1. Bottom-Up Engineering of Well-Defined 3D Microtissues Using Microplatforms and Biomedical Applications

      Geon Hui Lee, Jae Seo Lee, Xiaohong Wang and Sang Hoon Lee

      Article first published online: 16 APR 2015 | DOI: 10.1002/adhm.201500107

      Thumbnail image of graphical abstract

      Engineering of 3D tissues, which are building blocks for the bottom-up engineering, has been widely developed with the advances of micro-technologies. This article gives an overview of those micro-technologies that facilitate providing well defined 3D tissues and in vivo mimicking platforms, and reports the applications of 3D tissues and future directions.

  20. Full Papers

    1. Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering

      Xin Zhao, Qi Lang, Lara Yildirimer, Zhi Yuan Lin, Wenguo Cui, Nasim Annabi, Kee Woei Ng, Mehmet R. Dokmeci, Amir M. Ghaemmaghami and Ali Khademhosseini

      Article first published online: 16 APR 2015 | DOI: 10.1002/adhm.201500005

      Thumbnail image of graphical abstract

      A photocrosslinkable, biodegradable, naturally derived hydrogel, gelatin methacrylamide, with tunable mechanical and degradation properties, is developed for skin tissue regeneration. The engineered hydrogels can support keratinocyte adhesion, proliferation, and differentiation to form a multilayered epidermis with barrier functions.

    2. Intracellular Delivery of Bioactive Cargos to Hard-to-Transfect Cells Using Carbon Nanosyringe Arrays under an Applied Centrifugal g-Force

      Minsuk Choi, Sang Ho Lee, Won Bae Kim, Vipul Gujrati, Daejin Kim, Jinju Lee, Jae-Il Kim, Hyungjun Kim, Phei Er Saw and Sangyong Jon

      Article first published online: 2 APR 2015 | DOI: 10.1002/adhm.201400834

      Thumbnail image of graphical abstract

      The carbon nanosyringe arrays under an applied centrifugal g-force can deliver diverse bioactive cargos (small interfering RNAs, plasmids, proteins) directly into the cytosol of hard-to-transfect cells with high efficiency and reproducibility.

  21. Reviews

    1. You have free access to this content
      Engineering Stem Cells for Biomedical Applications

      Perry T. Yin, Edward Han and Ki-Bum Lee

      Article first published online: 13 MAR 2015 | DOI: 10.1002/adhm.201400842

      Thumbnail image of graphical abstract

      Engineered stem cells, which are stem cells that have been modified to enhance their intrinsic properties and to confer them with new functionalities, hold tremendous potential for the treatment of various human diseases and disorders. As such, this Review gives an in-depth account on the current status of engineered stem cells for various biomedical applications.

  22. Communications

    1. STAPLE: Stable Alginate Gel Prepared by Linkage Exchange from Ionic to Covalent Bonds

      Sang Hyeon Hong, Mikyung Shin, Junhee Lee, Ji Hyun Ryu, Suhee Lee, Jae Wook Yang, Wan Doo Kim and Haeshin Lee

      Article first published online: 11 MAR 2015 | DOI: 10.1002/adhm.201400833

      Thumbnail image of graphical abstract

      A new Ca2+-alginate hydrogel with improved physical stability is prepared by covalent tethering of catechols. Ionic to covalent bond transition spontaneously occurrs in physiological conditions. It is found that the kinetics of leaching out of Ca2+ ions from the chelated carboxylates and oxidative intercatechol cross-linking is similar, successfully maintaining the original physical shapes without conventionally performed treatment of cationic polymers on the surface Ca2+-alginate hydrogels.


  1. 1 - 42