© John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze
Online ISSN: 1600-0854
ORIGINAL ARTICLE: Phosphatidylinositol 3,5-Bisphosphate-Rich Membrane Domains in Endosomes and Lysosomes
ORIGINAL ARTICLE: The Sec1/Munc18 Protein Groove Plays a Conserved Role in Interaction with Sec9p/SNAP-25
ORIGINAL ARTICLE: Phosphorylation of αSNAP is Required for Secretory Organelle Biogenesis in Toxoplasma gondii
REVIEW: The Crossroads of Synaptic Growth Signaling, Membrane Traffic and Neurological Disease: Insights from Drosophila
Recently Published Articles
- Dynamin-actin cross-talk contributes to phagosome formation and closure
Florence Marie-Anaïs, Julie Mazzolini, Floriane Herit and Florence Niedergang
Accepted manuscript online: 5 FEB 2016 01:15AM EST | DOI: 10.1111/tra.12386
Phagosome formation relies on profound reorganization of actin and membranes, but the mechanism of phagosome closure remains poorly understood. We used an original experimental set up to monitor phagosome formation and closure in three dimensions in living macrophages using Total Internal Reflection Fluorescence (TIRF) Microscopy. We reveal that a crosstalk between actin and dynamin-2 takes place for phagosome formation and closure, and that dynamin-2 plays a critical role in the effective scission of phagosomes from the plasma membrane.
- Engineered tug-of-war between kinesin and dynein controls direction of microtubule transport in vivo
Karim Rezaul, Dipika Gupta, Irina Semenova, Kazuho Ikeda, Pavel Kraikivski, Ji Yu, Ann Cowan, Ilya Zaliapin and Vladimir Rodionov
Accepted manuscript online: 4 FEB 2016 02:47AM EST | DOI: 10.1111/tra.12385
Recruitment of external plus-end directed microtubule motor kinesin-1 to the surface of pigment granules transported to microtubule minus-ends by cytoplasmic dynein in melanophores creates a tug-of-war between opposing microtubule motors in vivo. Loading with kinesin-1 attenuates minus-end directed runs of pigment granules generated by dynein, and reverses the overall direction of their movement. Therefore in the absence of external signals, a tug-of-war between opposing microtubule motors is sufficient to control the directionality of microtubule transport in vivo.
- Space: a final frontier for vacuolar pathogens
Elizabeth Di Russo Case, Judith A. Smith, Thomas A. Ficht, James E. Samuel and Paul de Figueiredo
Accepted manuscript online: 4 FEB 2016 02:16AM EST | DOI: 10.1111/tra.12382
Intracellular bacteria must appropriate host vesicular traffic and membrane fusion events to build pathogen-specific niches. Here, we review the molecular mechanisms and trafficking pathways that drive two space allocation strategies of intracellular bacteria, the formation of tight and spacious pathogen-containing vacuoles. We relate bacterial modulation of vacuolar space to its impact on critical facets of intracellular parasitism and discuss the evolutionary drivers that may have shaped their replicative vacuoles.
- Structural Basis of Cargo Recognition by Unconventional Myosins in Cellular Trafficking
Jianchao Li, Qing Lu and Mingjie Zhang
Accepted manuscript online: 4 FEB 2016 02:13AM EST | DOI: 10.1111/tra.12383
Unconventional myosins play critical roles in many aspects of cellular tracking processes via binding to different cargo proteins as well as lipid vesicles. This review focuses on the structural basis of cargo recognitions and cargo binding-induced motor activity regulations of several unconventional myosins with prominent roles in cellular trafficking.
- Measuring Exocytosis Rate Using Corrected Fluorescence Recovery After Photoconversion
Nan Luo, An Yan and Zhenbiao Yang
Accepted manuscript online: 29 JAN 2016 04:38AM EST | DOI: 10.1111/tra.12380
An optical method is developed to measure the exocytosis rate of plasma membrane or extracellular matrix proteins. In this method, the protein-of-interest is tagged with a green-to-red photoconvertible fluorescent protein; after photoconverting a region-of-interest on the cell surface, exocytosis-dependent and independent trafficking events are tracked simultaneously for accurate determination of exocytosis rate.