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Cover image for Vol. 16 Issue 3

Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens, Sharon A. Tooze

Online ISSN: 1600-0854

Highlights

  • ORIGINAL ARTICLE: Rerouting of Plant Late Endocytic Trafficking Toward a Pathogen Interface

    ORIGINAL ARTICLE: Rerouting of Plant Late Endocytic Trafficking Toward a Pathogen Interface

    Rerouting of late endosomes to host–pathogen interface. Late endosomes, which recruit cargo from plasma membrane and TGN to the vacuole, are diverted toward haustoria. Upon activation, cell surface PRRs can be recruited at the host–pathogen interface via this route. EE: early endosome, LE: late endosome, TGN: trans-Golgi network, MVB: multi vesicular body, vac: vacuole, p: plastid, Cyt.: cytoplasm, PM: plasma membrane, n: nucleus, PRR: pattern recognition receptor, Ton.: tonoplast, EHM: extrahaustorial membrane, EHMx: extrahaustorial matrix.

  • ORIGINAL ARTICLE: LYST Controls the Biogenesis of the Endosomal Compartment Required for Secretory Lysosome Function

    ORIGINAL ARTICLE: LYST Controls the Biogenesis of the Endosomal Compartment Required for Secretory Lysosome Function

    Analysis of lytic granules in control and CHS patient CTLs by CLEM. Representative fluorescence CLEM image obtained from control (A, B) and CHS [P4 ] (C, D) CTLs. CTLs were used between days 15 and 20 of the culture. The cells were pulsed with WGA-488 (green) and chased for 18 h. A and C) from left to right: the electromicroscopy image, fluorescence image and overlay. B and D) magnified view of a lytic granule of (A) and (C), respectively. The ILVs were artificially visualized in red by image processing (B, D, top rows). Details of a region of green fluorescence (WGA-488, green) and red ILV signal are shown on a merged image and on the EM overlay (B, D, bottom right).

  • ORIGINAL ARTICLE: Atg23 and Atg27 Act at the Early Stages of Atg9 Trafficking in S. cerevisiae

    ORIGINAL ARTICLE: Atg23 and Atg27 Act at the Early Stages of Atg9 Trafficking in S. cerevisiae

    Proposed model of Atg9 trafficking to thePAS. Numbered arrows indicate steps in the Atg9 trafficking pathway. Beneath each arrow is the protein(s) or interaction that facilitates that particular step. Steps are (1) formation of Atg9 clusters within the Golgi, (2) budding of Atg9 clusters to form the Atg9 peripheral sites and (3) targeting of Atg9 peripheral sites to the PAS (shown here is the ‘minimal PAS’ that was reconstituted in the MKO strain). (4) Normally, Atg9 is excluded from vacuolar transport pathways; however, in the absence of Atg27, the defect in Atg9 clustering may allow some Atg9 to escape its normal trajectory and instead enter into a vacuolar targeting pathway.

  • ORIGINAL ARTICLE: Intrinsically Disordered Linker and Plasma Membrane-Binding Motif Sort Ist2 and Ssy1 to Junctions

    ORIGINAL ARTICLE: Intrinsically Disordered Linker and Plasma Membrane‐Binding Motif Sort Ist2 and Ssy1 to Junctions

    PM–ER contact sites are restored in the Δtether strain by Ssy1(167–283)-TM. A) The ER in the Δtether strain, which lacks 6 proteins that mediate the formation of PM–ER contact sites, is visualized with an ER marker fused to mCherry. In the Δtether strain, the ER accumulates in the cytoplasm. Upon expression of Ssy1(167–283)-TM cortical ER appeared colocalizing with the reporter protein (open arrow head), while in cells with no apparent expression of Ssy1(167–283)-TM (closed arrowhead), the ER still accumulates in the cytoplasm. Scale bar represents 5 µm. B) Ultrastructural analysis of the Δtether strain without (left panel) and with (right panel) expressing Ssy1(167–283)-TM. Cortical ER appears when Ssy1(167–283)-TM is expressed as is indicated with the arrow heads. CW, cell wall; ER, endoplasmic reticulum; M, mitochondria; N, nucleus; V, vacuole. Scale bars represent 200 nm.

  • ORIGINAL ARTICLE: Rerouting of Plant Late Endocytic Trafficking Toward a Pathogen Interface
  • ORIGINAL ARTICLE: LYST Controls the Biogenesis of the Endosomal Compartment Required for Secretory Lysosome Function
  • ORIGINAL ARTICLE: Atg23 and Atg27 Act at the Early Stages of Atg9 Trafficking in S. cerevisiae
  • ORIGINAL ARTICLE: Intrinsically Disordered Linker and Plasma Membrane‐Binding Motif Sort Ist2 and Ssy1 to Junctions

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Rab5 and Ndfip1 Are Involved in Pten Ubiquitination and Nuclear Trafficking
Yijia Li, Ley-Hian Low, Ulrich Putz, Choo-Peng Goh, Seong-Seng Tan and Jason Howitt

Depending on its localization, Pten (the central antagonist of PI3K signaling in the cytoplasm) is involved in many diverse cellular functions including controlling mitosis and DNA repair, cellular homeostasis, cell migration and/or cell proliferation. Balancing the cellular distribution of Pten is crucial to the function of the cell. Li and colleagues provide evidence that sorting of Pten to various organelles occurs in endosomes. Using bimolecular fluorescence complementation and dominant negative Rab5, they demonstrate that Rab5 and the E3 ligase adaptor protein Ndfip1 work together in to ubiquitinate Pten, which is required for its trafficking to the nucleus.


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