Genes to Cells

Cover image for Vol. 21 Issue 4

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Mitsuhiro Yanagida

Impact Factor: 2.805

ISI Journal Citation Reports © Ranking: 2014: 70/167 (Genetics & Heredity); 111/184 (Cell Biology)

Online ISSN: 1365-2443


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  1. Brief Reports

    1. Postnatal knockdown of dok-7 gene expression in mice causes structural defects in neuromuscular synapses and myasthenic pathology

      Takahiro Eguchi, Tohru Tezuka, Sadanori Miyoshi and Yuji Yamanashi

      Article first published online: 18 APR 2016 | DOI: 10.1111/gtc.12370

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      Dok-7 is required for the embryonic formation of neuromuscular junction (NMJ), an essential synapse for muscle contraction, and its genetic mutations underlie an NMJ disorder, DOK7 myasthenia. We demonstrate that postnatal knockdown of dok-7 gene expression in mice causes structural defects in NMJs and myasthenic symptoms including reduction in body weight and motor function. Thus, correct physiological levels of dok-7 expression are required for the postnatal maintenance of NMJs, implying its relevance in myasthenia.

  2. Original Articles

    1. Midori-ishi Cyan/monomeric Kusabira-Orange-based fluorescence resonance energy transfer assay for characterization of various E3 ligases

      Ryota Otsubo, Minsoo Kim, Jihye Lee and Chihiro Sasakawa

      Article first published online: 18 APR 2016 | DOI: 10.1111/gtc.12369

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      Our results demonstrate that the MiCy/mKO-based FRET assay is useful for characterizing E3 ligases, as well as for high-throughput E3 inhibitor screening.

    2. Anillin-related protein Mid1 regulates timely formation of the contractile ring in the fission yeast Schizosaccharomyces japonicus

      Tsuyoshi Yasuda, Masak Takaine, Osamu Numata and Kentaro Nakano

      Article first published online: 5 APR 2016 | DOI: 10.1111/gtc.12368

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      We investigated the role of S. japonicus Mid1 during mitosis to compare the molecular mechanisms that determine the cell division site in Schizosaccharomyces. Although the Mid1 dependency for positional control of the cell division site is possibly different between the fission yeast species, we found that Sj Mid1 enhanced CR formation, in a manner possibly similar to that by S. pombe Mid1.

    3. Mammalian target of rapamycin (mTOR) complex 2 regulates filamin A-dependent focal adhesion dynamics and cell migration

      Tatsuhiro Sato, Junko Ishii, Yuki Ota, Eri Sasaki, Yoshio Shibagaki and Seisuke Hattori

      Article first published online: 5 APR 2016 | DOI: 10.1111/gtc.12366

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      We found that filamin A, a for key regulator actin cytoskeletal integrity and cell migration, was phosphorylated at serine 2152 by mTORC2. mTORC2 regulates focal adhesion formation through the phosphorylation of S2152 of filamin A.

    4. Distribution of human single-nucleotide polymorphisms is approximated by the power law and represents a fractal structure

      Norio Gouda, Yuh Shiwa, Motohiro Akashi, Hirofumi Yoshikawa, Ken Kasahara and Mitsuru Furusawa

      Article first published online: 31 MAR 2016 | DOI: 10.1111/gtc.12344

  3. Brief Reports

    1. You have full text access to this OnlineOpen article
      R26-WntVis reporter mice showing graded response to Wnt signal levels

      Tatsuya Takemoto, Takaya Abe, Hiroshi Kiyonari, Kazuki Nakao, Yasuhide Furuta, Hitomi Suzuki, Shinji Takada, Toshihiko Fujimori and Hisato Kondoh

      Article first published online: 31 MAR 2016 | DOI: 10.1111/gtc.12364

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      We constructed a new Wnt signal reporter, in which HSV-tk promoter-driven histone H2B-EGFP was inserted in the ROSA26 locus. The R26-WntVis allele was introduced into Wnt3a−/− and Wnt3avt/− mutant mouse embryos and compared with wild type embryos to assess its performance. The R26-WntVis reporter was activated in known Wnt-dependent tissues and responded in a graded fashion to signal intensity.

  4. Original Articles

    1. Genomewide approaches for BACH1 target genes in mouse embryonic fibroblasts showed BACH1-Pparg pathway in adipogenesis

      Mitsuyo Matsumoto, Keiichi Kondo, Takuma Shiraki, Andrey Brydun, Ryo Funayama, Keiko Nakayama, Nobuo Yaegashi, Hideki Katagiri and Kazuhiko Igarashi

      Article first published online: 30 MAR 2016 | DOI: 10.1111/gtc.12365

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      Bach1-deficient immortalized MEFs (iMEFs) showed increased expression of mRNA and protein of PPARγ, which is the key factor of adipogenesis. These cells also showed a concomitant increase in ligand-dependent activation of PPARγ target genes compared with wild-type iMEFs. Moreover, Bach1-deficient iMEFs efficiently differentiated to adipocyte compared with wild-type cells in the presence of PPARγ ligands. Our results suggest that BACH1 regulates expression of adipocyte-related genes including Pparg and potentiates adipocyte differentiation capacity.

    2. Cilia play a role in breaking left–right symmetry of the sea urchin embryo

      Ayumi Takemoto, Tatsuo Miyamoto, Fumie Simono, Nao Kurogi, Maki Shirae-Kurabayashi, Akinori Awazu, Ken-ichi T. Suzuki, Takashi Yamamoto and Naoaki Sakamoto

      Article first published online: 29 MAR 2016 | DOI: 10.1111/gtc.12362

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      Ciliary motion is involved in the establishment of left–right asymmetry of sea urchin embryo. The initial cilia generated on small micromeres during the early stage of embryogenesis may be involved in this process.

    3. Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish

      Yoshinari Nakahara, Akihiko Muto, Ryo Hirabayashi, Tetsushi Sakuma, Takashi Yamamoto, Shoen Kume and Yutaka Kikuchi

      Article first published online: 29 MAR 2016 | DOI: 10.1111/gtc.12358

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      The adenohypophysis (AH), the anterior part of the pituitary gland, consists of six types of hormone-secreting cells and their cell fate is regulated by several signaling pathway during animal development. We found that Notch signaling is necessary for AH cell specification in wider time range than previously reported in zebrafish. We also found, by using knockdown and knockout zebrafish embryos, that multiple downstream effectors, including Her4.1 and Hey1, are likely to mediate Notch signaling during AH cell specification.

  5. Meeting Reports

    1. Report on the Conference on Transposition and Genome Engineering 2015 (TGE 2015): advancing cutting-edge genomics technology in the ancient city of Nara

      Knut Woltjen, Takashi Yamamoto, Chikara Kokubu and Junji Takeda

      Article first published online: 29 MAR 2016 | DOI: 10.1111/gtc.12367

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      Group photograph from the Conference on Transposition and Genome Engineering 2015 (TGE 2015) held at Nara Kasugano International Forum—IRAKA—in Nara, Japan on 17 to 20 November 2015.

  6. Original Articles

    1. Identification of YPL014W (Cip1) as a novel negative regulator of cyclin-dependent kinase in Saccharomyces cerevisiae

      Ping Ren, Asrar Malik and Fanli Zeng

      Article first published online: 23 MAR 2016 | DOI: 10.1111/gtc.12361

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      We report a novel Cdk1-interacting protein, YPL014W, which we name Cip1 (for Cdk1-interacting protein 1). Cip1 directly interacts and inhibits Cln2–CDK activity both in vivo and in vitro. Our finding proves Cip1 as a novel negative regulator of cyclin-dependent kinase in S. cerevisiae.

    2. Diverse fission yeast genes required for responding to oxidative and metal stress: Comparative analysis of glutathione-related and other defense gene deletions

      Tomáš Pluskal, Kenichi Sajiki, Joanne Becker, Kojiro Takeda and Mitsuhiro Yanagida

      Article first published online: 23 MAR 2016 | DOI: 10.1111/gtc.12359

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      We constructed a collection of twelve single-gene deletion strains of the fission yeast Schizosaccharomyces pombe designed for the study of oxidative and heavy metal stress responses.

    3. Serine suppresses the motor function of a periplasmic PomB mutation in the Vibrio flagella stator

      Tatsuro Nishikino, Shiwei Zhu, Norihiro Takekawa, Seiji Kojima, Yasuhiro Onoue and Michio Homma

      Article first published online: 23 MAR 2016 | DOI: 10.1111/gtc.12357

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      We found that the addition of serine, a chemotactic attractant, to a PomB(L160C/I186C) mutant restored motility without cleaving the disulfide bridge in Vibrio. Motility was restored without serine in Che mutants. We speculate that serine changed the rotor conformation to suppress the motor function of the PomB mutation.

  7. Brief Reports

    1. FBXL12 regulates T-cell differentiation in a cell-autonomous manner

      Akihiro Nita, Masaaki Nishiyama, Yoshiharu Muto and Keiichi I. Nakayama

      Article first published online: 21 MAR 2016 | DOI: 10.1111/gtc.12360

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      Model for the role of the FBXL12-ALDH3 axis in T-cell differentiation. FBXL12 mediates ubiquitin-dependent degradation of ALDH3 and thereby promotes the differentiation of DP cells into CD4 SP or CD8 SP cells.

  8. Original Articles

    1. Conserved interaction of Ctf18-RFC with DNA polymerase ε is critical for maintenance of genome stability in saccharomyces cerevisiae

      Hiroko Okimoto, Seiji Tanaka, Hiroyuki Araki, Eiji Ohashi and Toshiki Tsurimoto

      Article first published online: 14 MAR 2016 | DOI: 10.1111/gtc.12356

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      The specific interaction of Ctf18-RFC with Polε is a conserved feature between these proteins. We demonstrated that the interaction plays a crucial role in maintaining genome stability in budding yeast, probably through recruitment of this PCNA loader to the replication fork.

    2. Organ identity specification factor WGE localizes to the histone locus body and regulates histone expression to ensure genomic stability in Drosophila

      Nao Ozawa, Hirofumi Furuhashi, Keita Masuko, Eriko Numao, Takashi Makino, Tamaki Yano and Shoichiro Kurata

      Article first published online: 8 MAR 2016 | DOI: 10.1111/gtc.12354

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      Organ identity specification factor, Winged-Eye (WGE), localized to specific nuclear foci called the histone locus body (HLB), an evolutionarily conserved nuclear body required for S phase-specific histone mRNA production. We propose that WGE at HLB contributes to genomic stability and development by regulating heterochromatin structure via histone gene regulation.

    3. Glucose-regulated protein 78 (GRP78) binds directly to PIP3 phosphatase SKIP and determines its localization

      Takeshi Ijuin, Naoya Hatano and Tadaomi Takenawa

      Article first published online: 4 MAR 2016 | DOI: 10.1111/gtc.12353

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      SKIP is a PIP3 phosphatase that negatively regulates insulin signaling. SKIP directly binds to GRP78 through the SKICH domain, which is necessary for the localization of SKIP at ER. Dissociation from GRP78 on insulin stimulation allows SKIP to bind to Pak1 at the plasma membrane.

    4. Distinct types of protease systems are involved in homeostasis regulation of mitochondrial morphology via balanced fusion and fission

      Shotaro Saita, Takaya Ishihara, Maki Maeda, Shun-ichiro Iemura, Tohru Natsume, Katsuyoshi Mihara and Naotada Ishihara

      Article first published online: 3 MAR 2016 | DOI: 10.1111/gtc.12351

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      Mitochondria are dynamic organelles, but mechanism regulating balance between fusion and fission has not been clarified. In mitochondrial fission-deficient cells, mitochondrial fusion proteins were repressed. Mitofusins are degraded via ubiquitin-proteasome system mediated by BAT3, and L-OPA1 was processed via OMA1. Thus, distinct types of protease systems are involved in the homeostatic regulation of mitochondrial morphology.

    5. Mib1 modulates dynamin 2 recruitment via Snx18 to promote Dll1 endocytosis for efficient Notch signaling

      Makoto Okano, Hiromi Matsuo, Yuya Nishimura, Katsuto Hozumi, Saho Yoshioka, Ayako Tonoki and Motoyuki Itoh

      Article first published online: 28 FEB 2016 | DOI: 10.1111/gtc.12350

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      Dll1–Notch interaction promotes Mib1 ubiquitin ligase activity, and Dll1 is ubiquitinated by Mib1. The Mib1 ubiquitin ligase activity modulates the recruitment of dynamin 2 via Snx18 to the sites of Dll1 internalization and promotes Dll1 endocytosis. This process leads to mechanical dissociation of the NotchECD and the release of the NICD in adjacent cells.

    6. TDP-43 binds and transports G-quadruplex-containing mRNAs into neurites for local translation

      Akira Ishiguro, Nobuyuki Kimura, Yuto Watanabe, Sumiko Watanabe and Akira Ishihama

      Article first published online: 24 FEB 2016 | DOI: 10.1111/gtc.12352

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      In this study, we found that trans-activation response (TAR) DNA-binding 43-kDa protein (TDP-43), recognizes G-quadruplex-containing mRNAs and transports them up to neurites for local translation. The success was based on the first purification of TDP-43 in soluble dimer forms, allowing in vitro SELEX screening of target RNA molecules. Furthermore, we found that the loss-of-function mutation or over-expression of TDP-43 result in defect of intracellular transport of mRNAs in neural cells, thereby leading to the neurodegenerative disorder such as amyotrophic lateral sclerosis and frontotemporal lobar degeneration.


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