Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse
Yoshiki Tsuchiya, Yoichi Minami, Yasuhiro Umemura, Hitomi Watanabe, Daisuke Ono, Wataru Nakamura, Tomoyuki Takahashi, Sato Honma, Gen Kondoh, Toyojiro Matsuishi and Kazuhiro Yagita
Article first published online: 12 OCT 2015 | DOI: 10.1111/gtc.12305
Rett syndrome (RTT), a neurodevelopmental disorder that is caused by a mutation of MECP2, is frequently associated with abnormal sleep patterns and sleep-associated problems. Here, we generated Mecp2-deficient mice using the CRISPR/Cas9 system and show that the circadian oscillation of PER2Luc reporter expression in the suprachiasmatic nucleus (SCN) was significantly attenuated in Mecp2-deficient mice as well as their activity rhythms. These data indicate that Mecp2 deficiency abrogates the circadian pacemaking ability of the SCN.