Genes to Cells

Cover image for Vol. 21 Issue 9

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Mitsuhiro Yanagida

Impact Factor: 2.481

ISI Journal Citation Reports © Ranking: 2015: 85/165 (Genetics & Heredity); 123/187 (Cell Biology)

Online ISSN: 1365-2443

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  1. 1 - 14
  1. Brief Reports

    1. Dendritic transport element of human arc mRNA confers RNA degradation activity in a translation-dependent manner

      Kensuke Ninomiya, Mutsuhito Ohno and Naoyuki Kataoka

      Version of Record online: 23 SEP 2016 | DOI: 10.1111/gtc.12439

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      The human arc DTE in 3′ UTR has mRNA-destabilizing activity independent of nonsense-mediated mRNA decay (NMD). Destabilizing activity of the arc DTE is dependent on translation.

    2. Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus

      Tetsushi Sakuma, Keiichi Masaki, Hiromi Abe-Chayama, Keiji Mochida, Takashi Yamamoto and Kazuaki Chayama

      Version of Record online: 23 SEP 2016 | DOI: 10.1111/gtc.12437

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      We have created highly multiplexed CRISPR-Cas9 nuclease and nickase vectors targeting critical regions of the hepatitis B virus (HBV) genome and evaluated their functionality and safety in HepG2 cells. Both vectors could effectively suppress HBV DNA and antigens, and the small number of off-target mutations detected by deep sequencing analysis was extremely rare. Thus, this study provides a proof-of-concept demonstration of CRISPR-Cas9-mediated HBV inactivation, potentially contributing to a well-designed therapeutic approach for curing HBV patients.

  2. Original Articles

    1. DNA damage inhibits lateral root formation by up-regulating cytokinin biosynthesis genes in Arabidopsis thaliana

      La Ode Muhammad Muchdar Davis, Nobuo Ogita, Soichi Inagaki, Naoki Takahashi and Masaaki Umeda

      Version of Record online: 23 SEP 2016 | DOI: 10.1111/gtc.12436

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      Lateral roots (LRs) are an important organ for water and nutrient uptake from soil. However, the underlying mechanism controlling LR formation in response to external factors has remained largely unknown. In this study, we revealed that SOG1 transcription factor regulates DNA repair and cytokinin signaling separately and plays a key role in controlling LR formation under genotoxic stress.

  3. Brief Reports

    1. Development of mouse models of malignant phyllodes tumors by transplantation of syngeneic mammary gland cells expressing mutant H-Ras

      Yayoi Takamoto, Yoshimi Arima and Hideyuki Saya

      Version of Record online: 23 SEP 2016 | DOI: 10.1111/gtc.12435

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      Our work describes mouse models of malignant phyllodes tumors driven by the introduction of H-RasG12V. Mouse undifferentiated mammary gland cells were infected with a retrovirus encoding the human oncoprotein H-RasG12V. The infected cells were transplanted orthotopically into the mammary fat pads of syngeneic mice, and these transplanted cells formed tumors.

  4. Original Articles

    1. Terminal differentiation of cortical neurons rapidly remodels RanGAP-mediated nuclear transport system

      Kazushiro Fujiwara, Koichi Hasegawa, Masahiro Oka, Yoshihiro Yoneda and Kazuaki Yoshikawa

      Version of Record online: 22 SEP 2016 | DOI: 10.1111/gtc.12434

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      RanGAP-mediated nuclear transport system is rapidly remodeled during neuronal terminal differentiation of cortical progenitor cells. SUMO-modified RanGAP undergoes desumoylation and degradation, which impedes the nuclear import of the DNA replication initiation factor Cdc6.

    2. DNase γ, DNase I and caspase-activated DNase cooperate to degrade dead cells

      Ryo Koyama, Tomoya Arai, Marie Kijima, Shoko Sato, Shigetoshi Miura, Makoto Yuasa, Daisuke Kitamura and Ryushin Mizuta

      Version of Record online: 22 SEP 2016 | DOI: 10.1111/gtc.12433

    3. Four domains of Ada1 form a heterochromatin boundary through different mechanisms

      Kazuma Kamata, Kaori Shinmyozu, Jun-ichi Nakayama, Masanori Hatashita, Hiroyuki Uchida and Masaya Oki

      Version of Record online: 20 SEP 2016 | DOI: 10.1111/gtc.12421

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      Heterochromatin boundary function of Ada1 is functionally linked to proteasome processes and that the four relatively small regions in ADA1 form a boundary via different mechanisms.

    4. Perichromosomal protein Ki67 supports mitotic chromosome architecture

      Masatoshi Takagi, Toyoaki Natsume, Masato T. Kanemaki and Naoko Imamoto

      Version of Record online: 9 SEP 2016 | DOI: 10.1111/gtc.12420

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      We revealed that Ki67, a perichromosomal protein, has a role in the assembly and the maintenance of mitotic chromosome architecture, likely in collaboration with TopoIIα. We represented a new model in which mitotic chromosome architecture is supported both internally and externally.

    5. Kinase activity of endosomal kinase LMTK1A regulates its cellular localization and interactions with cytoskeletons

      Govinda Sharma, Koji Tsutsumi, Taro Saito, Akiko Asada, Kanae Ando, Mineko Tomomura and Shin-ichi Hisanaga

      Version of Record online: 7 SEP 2016 | DOI: 10.1111/gtc.12404

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      LMTK1A is a protein kinase regulating endosome transport in neurites in neurons. Different localization of wild type and kinase negative LMTK1A in the microtubule-rich shaft and actin-rich tip of neurites, respectively, imply its role in the track change from microtubule to actin filaments during the transport of endosomal vesicles.

    6. Phosphoproteomics analyses show subnetwork systems in T-cell receptor signaling

      Atsushi Hatano, Masaki Matsumoto and Keiichi I. Nakayama

      Version of Record online: 7 SEP 2016 | DOI: 10.1111/gtc.12406

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      We have identified a novel signaling network motif in which the DAG-Erk pathway and IP3-CN pathway determine protein phosphorylation status in a competitive manner during TCR signaling.

    7. Comprehensive behavioral study and proteomic analyses of CRMP2-deficient mice

      Haruko Nakamura, Naoya Yamashita, Ayuko Kimura, Yayoi Kimura, Hisashi Hirano, Hiroko Makihara, Yuko Kawamoto, Aoi Jitsuki-Takahashi, Kumiko Yonezaki, Kenkichi Takase, Tomoyuki Miyazaki, Fumio Nakamura, Fumiaki Tanaka and Yoshio Goshima

      Version of Record online: 1 SEP 2016 | DOI: 10.1111/gtc.12403

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      We for the first time generated crmp2−/− gene deficient mice. We found that Crmp2−/− mice showed some behavioral abnormalities in activity level, duration of social interaction time and contextual learning. Crmp2−/− mice showed increased mathamphetamine-induced ambulatory activity and serotonin release from the prefrontal cortex. *P < 0.05, **P < 0.01, compared to WT.

    8. Biophysical characterization of drug-resistant mutants of fibroblast growth factor receptor 1

      Kaito Yoza, Rika Himeno, Shinjiro Amano, Yoshihiro Kobashigawa, Shun Amemiya, Natsuki Fukuda, Hiroyuki Kumeta, Hiroshi Morioka and Fuyuhiko Inagaki

      Version of Record online: 25 AUG 2016 | DOI: 10.1111/gtc.12405

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      Affinity of drug-resistant mutants of FGFR1 for ATP-competitive inhibitors were evaluated by biophysical techniques. The only marked reduction in affinity was observed that of PD173074 for the gatekeeper mutant (V561M). The molecular brake mutant (N546K) exhibited increased affinity for the ATP-analogue. These findings will help to clarify the mechanism of drug-resistance in mutant tyrosine kinases.

    9. PKCη deficiency improves lipid metabolism and atherosclerosis in apolipoprotein E-deficient mice

      Kumiko Torisu, Xueli Zhang, Mari Nonaka, Takahide Kaji, Daisuke Tsuchimoto, Kosuke Kajitani, Kunihiko Sakumi, Takehiro Torisu, Kazuhiro Chida, Katsuo Sueishi, Michiaki Kubo, Jun Hata, Takanari Kitazono, Yutaka Kiyohara and Yusaku Nakabeppu

      Version of Record online: 22 AUG 2016 | DOI: 10.1111/gtc.12402

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      We found that the dyslipidemia observed in Prkch+/+Apoe−/− mice was improved in Prkch−/−Apoe−/− mice. HFD-induced liver steatosis was markedly attenuated in Prkch−/−Apoe−/− mice. Consistent with improvements of dyslipidemia, atherosclerotic lesions were decreased in HFD-fed Prkch−/−Apoe−/− mice.

  5. Brief Reports

    1. Application of NanoLuc to monitor the intrinsic promoter activity of GRP78 using the CRISPR/Cas9 system

      Kentaro Oh-hashi, Eri Furuta, Junpei Norisada, Fumimasa Amaya, Yoko Hirata and Kazutoshi Kiuchi

      Version of Record online: 12 AUG 2016 | DOI: 10.1111/gtc.12401

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      We applied a highly sensitive small luciferase, NanoLuc, to establish a knock-in cell line using the CRISPR/Cas9 system and characterized the endogenous promoter activity of the human GRP78 gene.

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