Genes to Cells

Cover image for Vol. 21 Issue 8

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Mitsuhiro Yanagida

Impact Factor: 2.481

ISI Journal Citation Reports © Ranking: 2015: 85/165 (Genetics & Heredity); 123/187 (Cell Biology)

Online ISSN: 1365-2443


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  1. Original Articles

    1. Biophysical characterization of drug-resistant mutants of fibroblast growth factor receptor 1

      Kaito Yoza, Rika Himeno, Shinjiro Amano, Yoshihiro Kobashigawa, Shun Amemiya, Natsuki Fukuda, Hiroyuki Kumeta, Hiroshi Morioka and Fuyuhiko Inagaki

      Version of Record online: 25 AUG 2016 | DOI: 10.1111/gtc.12405

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      Affinity of drug-resistant mutants of FGFR1 for ATP-competitive inhibitors were evaluated by biophysical techniques. The only marked reduction in affinity was observed that of PD173074 for the gatekeeper mutant (V561M). The molecular brake mutant (N546K) exhibited increased affinity for the ATP-analogue. These findings will help to clarify the mechanism of drug-resistance in mutant tyrosine kinases.

    2. PKCη deficiency improves lipid metabolism and atherosclerosis in apolipoprotein E-deficient mice

      Kumiko Torisu, Xueli Zhang, Mari Nonaka, Takahide Kaji, Daisuke Tsuchimoto, Kosuke Kajitani, Kunihiko Sakumi, Takehiro Torisu, Kazuhiro Chida, Katsuo Sueishi, Michiaki Kubo, Jun Hata, Takanari Kitazono, Yutaka Kiyohara and Yusaku Nakabeppu

      Version of Record online: 22 AUG 2016 | DOI: 10.1111/gtc.12402

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      We found that the dyslipidemia observed in Prkch+/+Apoe−/− mice was improved in Prkch−/−Apoe−/− mice. HFD-induced liver steatosis was markedly attenuated in Prkch−/−Apoe−/− mice. Consistent with improvements of dyslipidemia, atherosclerotic lesions were decreased in HFD-fed Prkch−/−Apoe−/− mice.

  2. Brief Reports

    1. Application of NanoLuc to monitor the intrinsic promoter activity of GRP78 using the CRISPR/Cas9 system

      Kentaro Oh-hashi, Eri Furuta, Junpei Norisada, Fumimasa Amaya, Yoko Hirata and Kazutoshi Kiuchi

      Version of Record online: 12 AUG 2016 | DOI: 10.1111/gtc.12401

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      We applied a highly sensitive small luciferase, NanoLuc, to establish a knock-in cell line using the CRISPR/Cas9 system and characterized the endogenous promoter activity of the human GRP78 gene.

  3. Original Articles

    1. Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K

      Koichi Yamamoto, Mari T. Furukawa, Kazuhiro Fukumura, Arisa Kawamura, Tomoko Yamada, Hitoshi Suzuki, Tetsuro Hirose, Hiroshi Sakamoto and Kunio Inoue

      Version of Record online: 5 AUG 2016 | DOI: 10.1111/gtc.12400

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      We found that RNA-binding proteins hnRNP K and PSF/SFPQ are necessary for the alternative splicing of HSP105 during heat stress. Our results showed that a group of genes is alternatively spliced during heat stress in an hnRNP K-dependent manner, whereas hnRNP K is not necessary for the stress-induced alternative splicing of the remaining genes. Among the latter group, we found that SRp38/SRSF10 and SC35/SRSF2 are necessary for the alternative splicing of TNRC6A upon heat stress.

    2. CRMP1 and CRMP2 have synergistic but distinct roles in dendritic development

      Hiroko Makihara, Shiori Nakai, Wataru Ohkubo, Naoya Yamashita, Fumio Nakamura, Hiroshi Kiyonari, Go Shioi, Aoi Jitsuki-Takahashi, Haruko Nakamura, Fumiaki Tanaka, Tomoko Akase, Pappachan Kolattukudy and Yoshio Goshima

      Version of Record online: 2 AUG 2016 | DOI: 10.1111/gtc.12399

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      In vitro finding suggests that both CRMP1 and CRMP2 are downstream molecules of Sema3A signaling. Dendritic spine density and branching were reduced in double-heterozygous sema3A+/−;crmp2+/− and sema3A+/−;crmp1+/− mice, but the phenotypic defects had no genetic interaction between crmp1 and crmp2. These findings suggest that both CRMP1 and CRMP2 mediate Sema3A signaling to regulate dendritic spine maturation and patterning, but through overlapping and distinct signaling pathways.

    3. Integrative analysis of transcriptome and miRNome unveils the key regulatory connections involved in different stages of hepatocellular carcinoma

      Vignesh Ramesh and Kumaresan Ganesan

      Version of Record online: 27 JUL 2016 | DOI: 10.1111/gtc.12396

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      Co-expression based microRNA network using multiple miRNA expression profiles showed the applicability of the miRNA networking approach and was found informative. Interestingly, integration with functionally defined mRNA network modules revealed key regulatory mRNA-miRNA circuits in HCC development and progression. The study provided an holistic view of transcriptome-miRNome at the modular level during HCC conditions.

    4. Characterization of a homologue of mammalian serine racemase from Caenorhabditis elegans: the enzyme is not critical for the metabolism of serine in vivo

      Masumi Katane, Yuki Saitoh, Keita Uchiyama, Kazuki Nakayama, Yasuaki Saitoh, Tetsuya Miyamoto, Masae Sekine, Kouji Uda and Hiroshi Homma

      Version of Record online: 26 JUL 2016 | DOI: 10.1111/gtc.12398

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      A homologue (T01H8.2) of mammalian serine racemase from Caenorhabditis elegans was characterized. The results demonstrate that T01H8.2 shows dehydratase activity toward several hydroxyamino acids in addition to racemase activity. Furthermore, the enzyme does not appear to metabolize d- and l-serine in vivo.

    5. You have full text access to this OnlineOpen article
      ICRF-193, an anticancer topoisomerase II inhibitor, induces arched telophase spindles that snap, leading to a ploidy increase in fission yeast

      Norihiko Nakazawa, Rajesh Mehrotra, Orie Arakawa and Mitsuhiro Yanagida

      Version of Record online: 26 JUL 2016 | DOI: 10.1111/gtc.12397

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      ICRF-193 is an inhibitor of DNA topoisomerase II that is used as an effective anticancer drug. Here, we examined the effects of ICRF-193 treatment on chromatin behavior and spindle dynamics using detailed live mitotic cell analysis in the fission yeast, Schizosaccharomyces pombe. We demonstrated that ICRF-193-treated, unseparated sister chromatids pulling toward opposite spindle poles produce the ‘arched’ and ‘snapped’ unique telophase spindle, resulting in polyploidization.

  4. Brief Reports

    1. Chromosomal location of the DnaA-reactivating sequence DARS2 is important to regulate timely initiation of DNA replication in Escherichia coli

      Yukie Inoue, Hiroyuki Tanaka, Kazutoshi Kasho, Kazuyuki Fujimitsu, Taku Oshima and Tsutomu Katayama

      Version of Record online: 25 JUL 2016 | DOI: 10.1111/gtc.12395

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      The DnaA-reactivating sequence 2, DARS2, on the chromosome forms specific nucleoprotein complexes, producing replication-active ATP-DnaA from inactive ADP-DnaA. We found that the chromosomal location of DARS2 is relevant to the initiation regulation of replication.

  5. Original Articles

    1. You have full text access to this OnlineOpen article
      Caenorhabditis elegans homologue of Prox1/Prospero is expressed in the glia and is required for sensory behavior and cold tolerance

      Eriko Kage-Nakadai, Akane Ohta, Tomoyo Ujisawa, Simo Sun, Yoshikazu Nishikawa, Atsushi Kuhara and Shohei Mitani

      Version of Record online: 12 JUL 2016 | DOI: 10.1111/gtc.12394

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      We performed RNA interference (RNAi) screening for transcription factors that regulate the expression of an amphid sheath glial cell marker and identified pros-1, which encodes a homeodomain transcription factor homologous to Drosophila prospero/mammalian Prox1, as a positive regulator. The functional PROS-1::EGFP fusion protein was localized in the nuclei of the glia and the excretory cell but not in the amphid sensory neurons. We further found that the structure and functions of sensory neurons, such as the morphology of sensory endings, sensory behavior and sensory-mediated cold tolerance, appeared to be affected by pros-1 RNAi.


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