Genes to Cells

Cover image for Vol. 21 Issue 7

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Mitsuhiro Yanagida

Impact Factor: 2.481

ISI Journal Citation Reports © Ranking: 2015: 85/165 (Genetics & Heredity); 123/187 (Cell Biology)

Online ISSN: 1365-2443


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  1. Original Articles

    1. Integrative analysis of transcriptome and miRNome unveils the key regulatory connections involved in different stages of hepatocellular carcinoma

      Vignesh Ramesh and Kumaresan Ganesan

      Version of Record online: 27 JUL 2016 | DOI: 10.1111/gtc.12396

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      Co-expression based microRNA network using multiple miRNA expression profiles showed the applicability of the miRNA networking approach and was found informative. Interestingly, integration with functionally defined mRNA network modules revealed key regulatory mRNA-miRNA circuits in HCC development and progression. The study provided an holistic view of transcriptome-miRNome at the modular level during HCC conditions.

    2. Characterization of a homologue of mammalian serine racemase from Caenorhabditis elegans: the enzyme is not critical for the metabolism of serine in vivo

      Masumi Katane, Yuki Saitoh, Keita Uchiyama, Kazuki Nakayama, Yasuaki Saitoh, Tetsuya Miyamoto, Masae Sekine, Kouji Uda and Hiroshi Homma

      Version of Record online: 26 JUL 2016 | DOI: 10.1111/gtc.12398

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      A homologue (T01H8.2) of mammalian serine racemase from Caenorhabditis elegans was characterized. The results demonstrate that T01H8.2 shows dehydratase activity toward several hydroxyamino acids in addition to racemase activity. Furthermore, the enzyme does not appear to metabolize d- and l-serine in vivo.

    3. You have full text access to this OnlineOpen article
      ICRF-193, an anticancer topoisomerase II inhibitor, induces arched telophase spindles that snap, leading to a ploidy increase in fission yeast

      Norihiko Nakazawa, Rajesh Mehrotra, Orie Arakawa and Mitsuhiro Yanagida

      Version of Record online: 26 JUL 2016 | DOI: 10.1111/gtc.12397

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      ICRF-193 is an inhibitor of DNA topoisomerase II that is used as an effective anticancer drug. Here, we examined the effects of ICRF-193 treatment on chromatin behavior and spindle dynamics using detailed live mitotic cell analysis in the fission yeast, Schizosaccharomyces pombe. We demonstrated that ICRF-193-treated, unseparated sister chromatids pulling toward opposite spindle poles produce the ‘arched’ and ‘snapped’ unique telophase spindle, resulting in polyploidization.

  2. Brief Reports

    1. Chromosomal location of the DnaA-reactivating sequence DARS2 is important to regulate timely initiation of DNA replication in Escherichia coli

      Yukie Inoue, Hiroyuki Tanaka, Kazutoshi Kasho, Kazuyuki Fujimitsu, Taku Oshima and Tsutomu Katayama

      Version of Record online: 25 JUL 2016 | DOI: 10.1111/gtc.12395

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      The DnaA-reactivating sequence 2, DARS2, on the chromosome forms specific nucleoprotein complexes, producing replication-active ATP-DnaA from inactive ADP-DnaA. We found that the chromosomal location of DARS2 is relevant to the initiation regulation of replication.

  3. Original Articles

    1. You have full text access to this OnlineOpen article
      Caenorhabditis elegans homologue of Prox1/Prospero is expressed in the glia and is required for sensory behavior and cold tolerance

      Eriko Kage-Nakadai, Akane Ohta, Tomoyo Ujisawa, Simo Sun, Yoshikazu Nishikawa, Atsushi Kuhara and Shohei Mitani

      Version of Record online: 12 JUL 2016 | DOI: 10.1111/gtc.12394

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      We performed RNA interference (RNAi) screening for transcription factors that regulate the expression of an amphid sheath glial cell marker and identified pros-1, which encodes a homeodomain transcription factor homologous to Drosophila prospero/mammalian Prox1, as a positive regulator. The functional PROS-1::EGFP fusion protein was localized in the nuclei of the glia and the excretory cell but not in the amphid sensory neurons. We further found that the structure and functions of sensory neurons, such as the morphology of sensory endings, sensory behavior and sensory-mediated cold tolerance, appeared to be affected by pros-1 RNAi.

    2. NEK6-mediated phosphorylation of human TPP1 regulates telomere length through telomerase recruitment

      Yugo Hirai, Miki Tamura, Junji Otani and Fuyuki Ishikawa

      Version of Record online: 11 JUL 2016 | DOI: 10.1111/gtc.12391

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      Human telomere protein TPP1 is phosphorylated in G2/M-phase cells by NEK6 kinase to regulate telomerase reactions.

  4. Brief Reports

    1. Balance between cAMP and Ca2+ signals regulates expression levels of pituitary adenylate cyclase-activating polypeptide gene in neurons

      Mamoru Fukuchi, Yuki Kuwana, Akiko Tabuchi and Masaaki Tsuda

      Version of Record online: 7 JUL 2016 | DOI: 10.1111/gtc.12393

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      Ca2+ signaling evoked by excitatory neurotransmission not only activates the Pacap promoter but also prevents Pacap mRNA degradation. In contrast, cAMP signals, which can be induced by Gs-coupled GPCR activation, reduce the expression levels of Pacap mRNA. However, cAMP signals also participate in the synergistic induction of Pacap in the presence of Ca2+ signals via calcineurin/CRTC1/CREB, resulting in robust PACAP expression in neurons.

  5. Original Articles

    1. Fluorescent-based evaluation of chaperone-mediated autophagy and microautophagy activities in cultured cells

      Masahiro Sato, Takahiro Seki, Ayumu Konno, Hirokazu Hirai, Yuki Kurauchi, Akinori Hisatsune and Hiroshi Katsuki

      Version of Record online: 5 JUL 2016 | DOI: 10.1111/gtc.12390

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      We established a novel method to monitor the activities of chaperone-mediated autophagy and microautophagy, separately, in cultured cell lines and primary cultured neurons.

  6. Brief Reports

    1. Targeting ID2 expression triggers a more differentiated phenotype and reduces aggressiveness in human salivary gland cancer cells

      Tomoki Sumida, Akiko Ishikawa, Hiroyuki Nakano, Tomohiro Yamada, Yoshihide Mori and Pierre-Yves Desprez

      Version of Record online: 1 JUL 2016 | DOI: 10.1111/gtc.12389

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      Inhibitors of DNA binding (ID) proteins are negative regulators of basic helix-loop-helix transcription factors, and generally stimulate cell proliferation and inhibit differentiation. ID2 knockdown triggers important changes in cell behavior, that is, it significantly reduces the expression of N-cadherin, vimentin and Snail, induces E-cadherin expression, and leads to a more differentiated phenotype exemplified by changes in cell shape. ID2 therefore represents a potential therapeutic target during salivary gland cancer progression. HSG, human salivary gland.

    2. Replication fork progression is paused in two large chromosomal zones flanking the DNA replication origin in Escherichia coli

      Masahiro Tatsumi Akiyama, Taku Oshima, Onuma Chumsakul, Shu Ishikawa and Hisaji Maki

      Version of Record online: 28 JUN 2016 | DOI: 10.1111/gtc.12388

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      We succeeded in determining a genomewide replication profile in Escherichia coli cells for the first time. We also found that replication fork progression is impeded at various loci on the E. coli chromosome, including two novel ∼200-kb DNA zones, L-fork-trapping zone (FTZ) and R-FTZ, that flank the replication origin in the growing cells. Forks are paused in the origin-proximal zones in response to thymidine limitation and transcription arrest. Horizontal axis; genome location (kb).

  7. Original Articles

    1. Synovial sarcoma cell lines showed reduced DNA repair activity and sensitivity to a PARP inhibitor

      Hiroyuki Yamasaki, Mamiko Miyamoto, Yuki Yamamoto, Tadashi Kondo, Toshiki Watanabe and Tsutomu Ohta

      Version of Record online: 28 JUN 2016 | DOI: 10.1111/gtc.12387

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      We have demonstrated that two synovial sarcoma cell lines have reduced HR repair activity and that they are sensitive to a PARP inhibitor. Moreover, treating with both a PARP inhibitor and an alkylating agent directly enhanced the therapeutic benefit in the treatment of synovial sarcoma. We also found that the recruitment of RAD51 at DSB sites was deficient in synovial sarcoma cell lines.

    2. You have full text access to this OnlineOpen article
      Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions

      Yoshie Tange, Yuji Chikashige, Shinya Takahata, Kei Kawakami, Masato Higashi, Chie Mori, Tomoko Kojidani, Yasuhiro Hirano, Haruhiko Asakawa, Yota Murakami, Tokuko Haraguchi and Yasushi Hiraoka

      Version of Record online: 23 JUN 2016 | DOI: 10.1111/gtc.12385

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      In the fission yeast, centromeric heterochromatin formation is augmented upon transfer to the rich medium in wild type cells, but this nutrition-dependent augmentation of heterochromatin is insufficient in the absence of Lem2. The phenotype is rescued by the additional expression of Lnp1.

    3. Cyclophilin PpiB is involved in motility and biofilm formation via its functional association with certain proteins

      Aggeliki Skagia, Chrysoula Zografou, Eleni Vezyri, Anastasia Venieraki, Panagiotis Katinakis and Maria Dimou

      Version of Record online: 16 JUN 2016 | DOI: 10.1111/gtc.12383

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      Cyclophilin PpiB negatively modulates swimming, swarming motility and biofilm formation ability of Escherichia coli. Many multicopy suppressors of each phenotype interact with PpiB, and in some cases, PpiB is able to functionally control these proteins.

  8. Brief Reports

    1. M-COPA, a novel Golgi system disruptor, suppresses apoptosis induced by Shiga toxin

      Takayuki Hattori, Miho Watanabe-Takahashi, Isamu Shiina, Yoshimi Ohashi, Shingo Dan, Kiyotaka Nishikawa, Takao Yamori and Mikihiko Naito

      Version of Record online: 15 JUN 2016 | DOI: 10.1111/gtc.12386

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      Shiga toxin (Stx) is a main virulence factor of Stx-producing Escherichia coli (STEC) that contributes to diarrhea and hemorrhagic colitis and occasionally to fatal systemic complications. We report here that 2-methylcoprophilinamide (M-COPA), a compound that induces disassembly of the Golgi apparatus by inactivating ADP-ribosylation factor 1 (Arf1), suppresses Stx-induced apoptosis.


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