R26-WntVis reporter mice showing graded response to Wnt signal levels
Tatsuya Takemoto, Takaya Abe, Hiroshi Kiyonari, Kazuki Nakao, Yasuhide Furuta, Hitomi Suzuki, Shinji Takada, Toshihiko Fujimori and Hisato Kondoh
Article first published online: 31 MAR 2016 | DOI: 10.1111/gtc.12364
We constructed a new Wnt signal reporter, in which HSV-tk promoter-driven histone H2B-EGFP was inserted in the ROSA26 locus. The R26-WntVis allele was introduced into Wnt3a−/− and Wnt3avt/− mutant mouse embryos and compared with wild type embryos to assess its performance. The R26-WntVis reporter was activated in known Wnt-dependent tissues and responded in a graded fashion to signal intensity.