Genes to Cells

Cover image for Vol. 20 Issue 11

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Mitsuhiro Yanagida

Impact Factor: 2.805

ISI Journal Citation Reports © Ranking: 2014: 70/167 (Genetics & Heredity); 111/184 (Cell Biology)

Online ISSN: 1365-2443


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  1. Original Articles

    1. MiR-29-mediated elastin down-regulation contributes to inorganic phosphorus-induced osteoblastic differentiation in vascular smooth muscle cells

      Ryo Sudo, Fumiaki Sato, Takuya Azechi and Hiroshi Wachi

      Article first published online: 27 NOV 2015 | DOI: 10.1111/gtc.12311

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      This basic study investigated the mechanism of elastin down-regulation in the progression of calcification, focusing on clarifying the effect of microRNA; miR-29 which is the regulator of elastin expression. We demonstrated that elastin down-regulation, which are regulated by miR-29, played a pivotal role in the progression of vascular smooth muscle cells calcification via osteoblastic differentiation.

    2. Distal regulatory element of the STAT1 gene potentially mediates positive feedback control of STAT1 expression

      Katsutoshi Yuasa and Takao Hijikata

      Article first published online: 23 NOV 2015 | DOI: 10.1111/gtc.12316

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      A novel distal regulatory element 5.5URR could mediate positive feedback regulation of the STAT1 gene expression. Through this autoregulation, the 5.5URR could also contribute to the amplification and/or prolongation of intracellular IFN signals.

    3. Effects of 6-meals-a-day feeding and 6-meals-a-day feeding combined with adrenalectomy on daily gene expression rhythms in rat epididymal white adipose tissue

      Yan Su, Ewout Foppen, Zhi Zhang, Eric Fliers and Andries Kalsbeek

      Article first published online: 15 NOV 2015 | DOI: 10.1111/gtc.12315

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      We investigated the importance of adrenal hormones and a daily feeding rhythm as rhythmic outputs of the biological clock to synchronize day/night rhythms in (clock) gene expression in white adipose tissue (WAT). We found that at least one of these two outputs should be present in order for WAT clock gene rhythms to be maintained.

    4. Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines

      Nguyen Ngoc Hoa, Remi Akagawa, Tomomi Yamasaki, Kouji Hirota, Kentaro Sasa, Toyoaki Natsume, Junya Kobayashi, Tetsushi Sakuma, Takashi Yamamoto, Kenshi Komatsu, Masato T. Kanemaki, Yves Pommier, Shunichi Takeda and Hiroyuki Sasanuma

      Article first published online: 2 NOV 2015 | DOI: 10.1111/gtc.12310

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      Using genome-editing technology, we here engineered the nuclease mutants of human TK6 cell line, which are involved in DNA double-strand break repair. Among the mutants, we found that CtIP and Dna2 predominantly function in the process of DSB resection to ensure efficient DSB repair by HR in human cells.

    5. You have full text access to this OnlineOpen article
      Casein kinase 1γ acts as a molecular switch for cell polarization through phosphorylation of the polarity factor Tea1 in fission yeast

      Takayuki Koyano, Karin Barnouin, Ambrosius P. Snijders, Kazunori Kume, Dai Hirata and Takashi Toda

      Article first published online: 2 NOV 2015 | DOI: 10.1111/gtc.12309

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      When Tea1 is entrapped by Cki3 to the cell membrane, Tea1 is hyperphosphorylated, thereby becoming inactive for the potentiation of growth from the cell tip. Under this condition, interaction between Tea1 and Tea4 is compromised. Phosphomimetic Tea1 recapitulates the nongrowth phenotype.

    6. Microphthalmia-associated transcription factor is expressed in projection neurons of the mouse olfactory bulb

      Koji Ohba, Kazuhisa Takeda, Hiroaki Yamamoto and Shigeki Shibahara

      Article first published online: 2 NOV 2015 | DOI: 10.1111/gtc.12312

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      Immunohistochemical analysis of the olfactory bulb (OB) revealed that Mitf-positive cells were detected near the glomerular layer (GL) and in the mitral cell layer (MCL). Arrowheads indicate the two types of Mitf-positive cells: mitral cells (closed arrowheads) and tufted cells (open arrowheads).

    7. You have full text access to this OnlineOpen article
      Neural cells play an inhibitory role in pancreatic differentiation of pluripotent stem cells

      Ryutaro Nakashima, Mayu Morooka, Nobuaki Shiraki, Daisuke Sakano, Soichiro Ogaki, Kazuhiko Kume and Shoen Kume

      Article first published online: 30 OCT 2015 | DOI: 10.1111/gtc.12308

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      Neural cells exhibits inhibitory signal for pancreatic differentiation from pluripotent stem cells. The neural cells inhibits Wnt signal. Release of this neural derived signal against Wnt, potentiated pancreatic differentiation.

  2. Meeting Reports

    1. Fission yeast meets a legend in Kobe: report of the Eighth International Fission Yeast Meeting

      Haruhiko Asakawa, Takaharu G. Yamamoto and Yasushi Hiraoka

      Article first published online: 19 OCT 2015 | DOI: 10.1111/gtc.12307

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      Ikuta Shrine, the meeting venue.

  3. Original Articles

    1. You have free access to this content
      Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse

      Yoshiki Tsuchiya, Yoichi Minami, Yasuhiro Umemura, Hitomi Watanabe, Daisuke Ono, Wataru Nakamura, Tomoyuki Takahashi, Sato Honma, Gen Kondoh, Toyojiro Matsuishi and Kazuhiro Yagita

      Article first published online: 12 OCT 2015 | DOI: 10.1111/gtc.12305

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      Rett syndrome (RTT), a neurodevelopmental disorder that is caused by a mutation of MECP2, is frequently associated with abnormal sleep patterns and sleep-associated problems. Here, we generated Mecp2-deficient mice using the CRISPR/Cas9 system and show that the circadian oscillation of PER2Luc reporter expression in the suprachiasmatic nucleus (SCN) was significantly attenuated in Mecp2-deficient mice as well as their activity rhythms. These data indicate that Mecp2 deficiency abrogates the circadian pacemaking ability of the SCN.

    2. Differential binding of ppGpp and pppGpp to E. coli RNA polymerase: photo-labeling and mass spectral studies

      Kirtimaan Syal and Dipankar Chatterji

      Article first published online: 8 OCT 2015 | DOI: 10.1111/gtc.12304

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      ppGpp and pppGpp have an overlapping binding site on RNA polymerase (RNAP). 8-azido ppGpp is mapped on β’ and β subunit of RNAP. 8-azido pppGpp is mapped on β’ subunit of RNAP.

    3. Interaction of human mitochondrial transcription factor A in mitochondria: its involvement in the dynamics of mitochondrial DNA nucleoids

      Katsumi Kasashima and Hitoshi Endo

      Article first published online: 7 OCT 2015 | DOI: 10.1111/gtc.12306

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      We visualized the interaction of TFAM molecules in mitochondria by the Kusabira-Green reporter. The TFAM interaction is involved in the dynamics of mitochondrial nucleoids.

    4. 7-Ketocholesterol-induced lysosomal dysfunction exacerbates vascular smooth muscle cell calcification via oxidative stress

      Ryo Sudo, Fumiaki Sato, Takuya Azechi and Hiroshi Wachi

      Article first published online: 30 SEP 2015 | DOI: 10.1111/gtc.12301

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      In our in vitro study, we demonstrated that the lysosomal-dysfunction-dependent oxidative stress played a pivotal role in the progression of vascular smooth muscle cells calcification by a low concentration of 7-ketocholesterol, and that even normal serum levels of 7-ketocholesterol can be a risk factor for vascular calcification.

    5. Genomic confirmation of nutrient-dependent mutability of mutators in Escherichia coli

      Saburo Tsuru, Yuuka Ishizawa, Atsushi Shibai, Yusuke Takahashi, Daisuke Motooka, Shota Nakamura and Tetsuya Yomo

      Article first published online: 28 SEP 2015 | DOI: 10.1111/gtc.12300

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      We used two mutator strains of Escherichia coli to explore the nutrient dependence of mutation rates at the genomic level. These strains were transferred repeatedly under different nutritional conditions for hundreds of generations to accumulate mutations. Whole-genome sequencing of the offspring revealed that the nutrient dependence of the mutation rates was pervasive at the genomic scale.


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