Genes to Cells

Cover image for Vol. 21 Issue 5

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Mitsuhiro Yanagida

Impact Factor: 2.805

ISI Journal Citation Reports © Ranking: 2014: 70/167 (Genetics & Heredity); 111/184 (Cell Biology)

Online ISSN: 1365-2443

VIEW

  1. 1 - 16
  1. Original Articles

    1. Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders

      Mitsuki Shigeta, Yuto Sakane, Midori Iida, Miyuki Suzuki, Keiko Kashiwagi, Akihiko Kashiwagi, Satoshi Fujii, Takashi Yamamoto and Ken-ichi T. Suzuki

      Version of Record online: 24 MAY 2016 | DOI: 10.1111/gtc.12379

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      Targeted gene disruption of tyrosinase (tyr), solute carrier family 45 member 2 (slc45a2) and leukocyte tyrosine kinase (ltk) using Cas9 protein or Cas9 mRNA with sgRNA in X. tropicalis founders.

    2. You have full text access to this OnlineOpen article
      Parametric analysis of RNA-seq expression data

      Tomokazu Konishi

      Version of Record online: 20 MAY 2016 | DOI: 10.1111/gtc.12372

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      Here, introduced a parametric method to normalize RNA-seq data, confirming lognormal distribution of read counts. The level of noise in recent data was much higher than those of microarrays. Primary cause of noise was not on the principle of the methodology, but might be on unintended RNA degradation through wet process.

    3. Endocannabinoid signaling regulates regenerative axon navigation in Caenorhabditis elegans via the GPCRs NPR-19 and NPR-32

      Strahil Iv. Pastuhov, Kunihiro Matsumoto and Naoki Hisamoto

      Version of Record online: 19 MAY 2016 | DOI: 10.1111/gtc.12377

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      We found that the excessive amount of endocannabinoid anandamide (AEA), which is produced by NAPE-1 and NAPE-2, inhibits the axon regeneration response of motor neurons after laser axotomy via the G protein-coupled receptors (GPCRs) NPR-19 and NPR-32 in Caenorhabditis elegans. Further analysis revealed that the AEA signaling is required for the avoidant response of growing axons at the injury site.

    4. Quantification of the HIV transcriptional activator complex in live cells by image-based protein–protein interaction analysis

      Kaori Asamitsu, Katsumi Omagari, Tomoya Okuda, Yurina Hibi and Takashi Okamoto

      Version of Record online: 19 MAY 2016 | DOI: 10.1111/gtc.12375

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      Here we describe the efficacy of novel method, Fluoppi, detecting the Tat:CycT1:CDK9 tri-molecular interaction in live cells. We found addition of Tat significantly stabilizes this trimolecular complex. The specificity of such effects has been confirmed by mutants of relevant proteins.

    5. Identification and molecular docking studies for novel inverse agonists of SREB, super conserved receptor expressed in brain

      Toshihiro Yanai, Aya Kurosawa, Yoshiaki Nikaido, Nozomi Nakajima, Tamio Saito, Hiroyuki Osada, Ayumu Konno, Hirokazu Hirai and Shigeki Takeda

      Version of Record online: 17 MAY 2016 | DOI: 10.1111/gtc.12378

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      One of the orphan GPCR subfamilies, Super conserved Receptor Expressed in Brain (SREB), consists of GPR27, GPR85 and GPR173 and is expressed in the central nervous system. We report the identification of inverse agonists for the SREB family without their agonists.

    6. CCL11-induced eosinophils inhibit the formation of blood vessels and cause tumor necrosis

      Yanjiang Xing, Yijun Tian, Takamasa Kurosawa, Sayaka Matsui, Maki Touma, Takanori Yanai, Qiong Wu and Kenkichi Sugimoto

      Version of Record online: 12 MAY 2016 | DOI: 10.1111/gtc.12371

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      MS-K-CCL11-conditioned medium (CM) and recombinant CCL11 induced eosinophil differentiation and effectively recruited the differentiated eosinophils. The eosinophils damaged the MS-K, NFSA and endothelial cells in a dose-dependent manner. CCL11 was responsible for the limited angiogenesis and necrosis by inducing and attracting eosinophils in the tumors.

    7. ΔNp63α controls YB-1 protein stability: evidence on YB-1 as a new player in keratinocyte differentiation

      Orsola di Martino, Annaelena Troiano, Andrea M. Guarino, Alessandra Pollice, Maria Vivo, Girolama La Mantia and Viola Calabrò

      Version of Record online: 11 MAY 2016 | DOI: 10.1111/gtc.12373

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      The increase in YB-1 oncoprotein level in the cell or YB-1 translocation from the cytoplasm to the nucleus is characteristic of malignant cell growth. ΔNp63α reduces YB-1 protein turnover and leads to accumulation of ubiquitin-conjugated YB-1 into the nucleus. Reduction of YB-1 protein level after treatment with a DNA-damaging agent is inhibited by ΔNp63α suggesting that YB-1 and ΔNp63α interplay can support keratinocyte proliferation and protect cells from apoptosis under genotoxic stress.

  2. Brief Reports

    1. Postnatal knockdown of dok-7 gene expression in mice causes structural defects in neuromuscular synapses and myasthenic pathology

      Takahiro Eguchi, Tohru Tezuka, Sadanori Miyoshi and Yuji Yamanashi

      Version of Record online: 18 APR 2016 | DOI: 10.1111/gtc.12370

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      Dok-7 is required for the embryonic formation of neuromuscular junction (NMJ), an essential synapse for muscle contraction, and its genetic mutations underlie an NMJ disorder, DOK7 myasthenia. We demonstrate that postnatal knockdown of dok-7 gene expression in mice causes structural defects in NMJs and myasthenic symptoms including reduction in body weight and motor function. Thus, correct physiological levels of dok-7 expression are required for the postnatal maintenance of NMJs, implying its relevance in myasthenia.

  3. Original Articles

    1. Midori-ishi Cyan/monomeric Kusabira-Orange-based fluorescence resonance energy transfer assay for characterization of various E3 ligases

      Ryota Otsubo, Minsoo Kim, Jihye Lee and Chihiro Sasakawa

      Version of Record online: 18 APR 2016 | DOI: 10.1111/gtc.12369

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      Our results demonstrate that the MiCy/mKO-based FRET assay is useful for characterizing E3 ligases, as well as for high-throughput E3 inhibitor screening.

    2. Anillin-related protein Mid1 regulates timely formation of the contractile ring in the fission yeast Schizosaccharomyces japonicus

      Tsuyoshi Yasuda, Masak Takaine, Osamu Numata and Kentaro Nakano

      Version of Record online: 5 APR 2016 | DOI: 10.1111/gtc.12368

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      We investigated the role of S. japonicus Mid1 during mitosis to compare the molecular mechanisms that determine the cell division site in Schizosaccharomyces. Although the Mid1 dependency for positional control of the cell division site is possibly different between the fission yeast species, we found that Sj Mid1 enhanced CR formation, in a manner possibly similar to that by S. pombe Mid1.

    3. Mammalian target of rapamycin (mTOR) complex 2 regulates filamin A-dependent focal adhesion dynamics and cell migration

      Tatsuhiro Sato, Junko Ishii, Yuki Ota, Eri Sasaki, Yoshio Shibagaki and Seisuke Hattori

      Version of Record online: 5 APR 2016 | DOI: 10.1111/gtc.12366

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      We found that filamin A, a for key regulator actin cytoskeletal integrity and cell migration, was phosphorylated at serine 2152 by mTORC2. mTORC2 regulates focal adhesion formation through the phosphorylation of S2152 of filamin A.

  4. Brief Reports

    1. You have full text access to this OnlineOpen article
      R26-WntVis reporter mice showing graded response to Wnt signal levels

      Tatsuya Takemoto, Takaya Abe, Hiroshi Kiyonari, Kazuki Nakao, Yasuhide Furuta, Hitomi Suzuki, Shinji Takada, Toshihiko Fujimori and Hisato Kondoh

      Version of Record online: 31 MAR 2016 | DOI: 10.1111/gtc.12364

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      We constructed a new Wnt signal reporter, in which HSV-tk promoter-driven histone H2B-EGFP was inserted in the ROSA26 locus. The R26-WntVis allele was introduced into Wnt3a−/− and Wnt3avt/− mutant mouse embryos and compared with wild type embryos to assess its performance. The R26-WntVis reporter was activated in known Wnt-dependent tissues and responded in a graded fashion to signal intensity.

  5. Original Articles

    1. Genomewide approaches for BACH1 target genes in mouse embryonic fibroblasts showed BACH1-Pparg pathway in adipogenesis

      Mitsuyo Matsumoto, Keiichi Kondo, Takuma Shiraki, Andrey Brydun, Ryo Funayama, Keiko Nakayama, Nobuo Yaegashi, Hideki Katagiri and Kazuhiko Igarashi

      Version of Record online: 30 MAR 2016 | DOI: 10.1111/gtc.12365

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      Bach1-deficient immortalized MEFs (iMEFs) showed increased expression of mRNA and protein of PPARγ, which is the key factor of adipogenesis. These cells also showed a concomitant increase in ligand-dependent activation of PPARγ target genes compared with wild-type iMEFs. Moreover, Bach1-deficient iMEFs efficiently differentiated to adipocyte compared with wild-type cells in the presence of PPARγ ligands. Our results suggest that BACH1 regulates expression of adipocyte-related genes including Pparg and potentiates adipocyte differentiation capacity.

    2. Cilia play a role in breaking left–right symmetry of the sea urchin embryo

      Ayumi Takemoto, Tatsuo Miyamoto, Fumie Simono, Nao Kurogi, Maki Shirae-Kurabayashi, Akinori Awazu, Ken-ichi T. Suzuki, Takashi Yamamoto and Naoaki Sakamoto

      Version of Record online: 29 MAR 2016 | DOI: 10.1111/gtc.12362

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      Ciliary motion is involved in the establishment of left–right asymmetry of sea urchin embryo. The initial cilia generated on small micromeres during the early stage of embryogenesis may be involved in this process.

    3. Identification of YPL014W (Cip1) as a novel negative regulator of cyclin-dependent kinase in Saccharomyces cerevisiae

      Ping Ren, Asrar Malik and Fanli Zeng

      Version of Record online: 23 MAR 2016 | DOI: 10.1111/gtc.12361

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      We report a novel Cdk1-interacting protein, YPL014W, which we name Cip1 (for Cdk1-interacting protein 1). Cip1 directly interacts and inhibits Cln2–CDK activity both in vivo and in vitro. Our finding proves Cip1 as a novel negative regulator of cyclin-dependent kinase in S. cerevisiae.

    4. Diverse fission yeast genes required for responding to oxidative and metal stress: Comparative analysis of glutathione-related and other defense gene deletions

      Tomáš Pluskal, Kenichi Sajiki, Joanne Becker, Kojiro Takeda and Mitsuhiro Yanagida

      Version of Record online: 23 MAR 2016 | DOI: 10.1111/gtc.12359

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      We constructed a collection of twelve single-gene deletion strains of the fission yeast Schizosaccharomyces pombe designed for the study of oxidative and heavy metal stress responses.

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