Journal of Microscopy

Cover image for Vol. 254 Issue 2

Edited By: Tony Wilson

Impact Factor: 1.633

ISI Journal Citation Reports © Ranking: 2012: 5/9 (Microscopy)

Online ISSN: 1365-2818

Author Guidelines

The Journal of Microscopy publishes top quality review articles, original research articles, Hot Topic papers (fast-tracked short communications),and letters to the Editor, covering all aspects of microscopy and analysis.
Manuscripts will be considered only on the understanding that they have not been published and are not being considered for publication elsewhere. Papers will be published only on the recommendation of suitable referees. Authors must affirm that they have been engaged in the conception and design of an investigation and/or the analysis and interpretation of the data, that they have helped in the drafting of the manuscript and that they have seen the final version.
'Hot Topic - fast-tracked short communication' papers MUST be topical, concise (shorter than original research articles), and of high interest/impact to the microscopy community. The Editor reserves the right to decide if a submitted article is a Hot Topic paper.

Submission to the Journal of Microscopy
Manuscripts should be submitted online at
Full instructions are available on the site and a user ID and password can be obtained on the first visit.
For technical support click on the “Get Help Now” link at the top right of any page.

Manuscript text and tables should ideally be submitted as Word documents. However .rtf or LaTeX files can also be accepted. Figures and images should be submitted as individual files. See the below section (Preparation of Electronic Artwork) for guidelines on the preparation of figures and image files. Supporting Information, such as movies, can also be submitted.

All Word files submitted will automatically be converted to PDF for review purposes. If LaTeX files are submitted, a PDF generated by the author must also be uploaded.
For any query related to online submission or to the status of a submitted manuscript, please contact the Editorial Office, e-mail:

Manuscript Style
Please ensure your manuscript follows the below guidelines:
• Contact details (postal address, email, telephone and fax) for the corresponding author should appear on the first page.
• Manuscript text should be double spaced and in one column.
• Use a standard font (e.g. sans serif, true-type fonts, preferably Arial, or Times (New) Roman if serif fonts required) at a size of 12 pt.
• Manuscripts should include a brief summary/abstract, which accurately summarises the content and is intelligible without reference to the text.
• The hierarchy of headings within the text should be clear; e.g. upper case bold for heading 1, lower case bold for heading 2, italics for heading 3, etc… 
• Spelling should conform with The Concise Oxford Dictionary. SI units must be used.
• Abbreviations should be used sparingly and only if a lengthy name or expression is repeated throughout the manuscript. When used, the abbreviated name or expression should be cited in full at first usage, followed by the accepted abbreviation in parentheses.
• Manufacturer name, town, state (if USA) and country should be given for equipment mentioned in the manuscript.
• Tables should appear on a separate page and must fit on a single page.
• Include up to six keywords that describe your paper for indexing purposes.

Addition of Second Abstract -- Lay Description: In order to make the science we publish more accessible to the non-expert or lay reader the Editors of the Journal of Microscopy have decided that a second abstract in easy to understand non-technical language should accompany every paper submitted to the Journal. These will be published on line on the Journal website and also in hard copy in “in focus” the Royal Microscopical Society magazine. Please submit with your paper an easy to understand abstract of no more than 400 words along with the standard abstract. The abstract may contain links to any on line resources that the authors may wish to make publically available and are related to their research. For more information on this outreach initiative please read Gunter C, Osterrieder A. Genome Biol. 2012 Aug 31;13(8):168. “A modest proposal for an outreach section in scientific publications”.

References should be in the form Joy (2000) or Joy, Smith & Williams (2000). For eight or more authors, use the form Echlin et al. (2000).
The reference list should:
• be listed in alphabetical order of first authors' names.
• (where a journal is cited) - include authors' surnames and initials, date of publication, title of paper, name of journal in full, volume number (and issue number where volume pages do not run on), and first and last page numbers. 
• (where a book is cited) - include authors' surnames and initials, title of book, year of publication, edition if relevant, followed by the publisher and town, county/state (and country if necessary) of publication.

Examples of reference style:
Ashford, A.E. (1998) Dynamic pleiomorphic vacuole systems: are they endosomes and transport compartments in fungal hyphae? Adv. Bot. Res. 28, 119-159.
Causton, B. (1984) The choice of resins for electron immunocytochemistry. Immunolabelling for Electron Microscopy (ed. by J. M. Polak and I. M. Varndell), pp. 29-36. Elsevier, Amsterdam.
Muller, C. (1966) Spherical Harmonics. Springer-Verlag, Berlin.
Fischer-Parton, S. (1999) Role of pH, calcium and vesicle trafficking in regulating hyphal tip growth of Neurospora crassa.
PhD Thesis, University of Edinburgh, Edinburgh.

References in Articles
We recommend the use of a tool such as EndNote or Reference Manager for reference management and formatting.
EndNote reference styles can be searched for here:
Reference Manager reference styles can be searched for here:

Preparation of Electronic Artwork
Please prepare your illustrations in accordance with the Electronic Artwork Guidelines.
All images/illustrations must be high resolution - a minimum resolution of 300 dpi.
Graphs, charts and maps should be supplied as .eps files (Encapsulated Postscript Format).
EPS files should be saved with fonts embedded.
(Packages which can be used to create EPS files include: Adobe Illustrator 7.0 and above, Deneba Canvas 6.0 and above, CorelDRAW 7.0 and above, SigmaPlot 8.01 and above).
Photographs/halftones/scanned images should be supplied as .tiff (Tagged Image File Format).
(Programs which can be used to create TIFF files include: Adobe Photoshop 4.0 and above, Adobe Illustrator 9.0 and GraphPad Prism 3).
Scanned images: To ensure adequate reproduction the scanning resolution should be:
lineart >800 dpi
half-tones >300 dpi
Figures containing halftone and line images >600 dpi
• Black and white images should be supplied as 'grayscale'.
• Colour images should be supplied as RGB.
• Multipart figures should be supplied in the final layout in one file, labelled (A), (B) etc.
• Supply figures at final size widths if possible: 19 picas (single column) or 40 picas (double column).
• Use sans serif, true-type fonts for labels if possible, preferably Arial or Helvetica, or Times (New) Roman if serif fonts required.
• Ensure all lines and lettering are clear
Click here for further information on the Preparation of electronic artwork.

If your paper is accepted, the author identified as the formal corresponding author for the paper will receive an email prompting them to login into Author Services; where via the Wiley Author Licensing Service (WALS) they will be able to complete the license agreement on behalf of all authors on the paper.

For authors signing the copyright transfer agreement
If the OnlineOpen option is not selected the corresponding author will be presented with the copyright transfer agreement (CTA) to sign. The terms and conditions of the CTA can be previewed in the samples associated with the Copyright FAQs below:
CTA Terms and Conditions

For authors choosing OnlineOpen
If the OnlineOpen option is selected the corresponding author will have a choice of the following Creative Commons License Open Access Agreements (OAA):

  • Creative Commons Attribution License OAA
  • Creative Commons Attribution Non-Commercial License OAA
  • Creative Commons Attribution Non-Commercial -NoDerivs License OAA

To preview the terms and conditions of these open access agreements please visit the Copyright FAQs hosted on Wiley Author Services and visit

If you select the OnlineOpen option and your research is funded by The Wellcome Trust and members of the Research Councils UK (RCUK) you will be given the opportunity to publish your article under a CC-BY license supporting you in complying with Wellcome Trust and Research Councils UK requirements. For more information on this policy and the Journal’s compliant self-archiving policy please visit:

The corresponding author will receive an email alert containing a link to a website. A working e-mail address must therefore be provided for the corresponding author. Acrobat Reader will be required in order to read this file. This software can be downloaded (free of charge) from Adobe. This will enable the file to be opened, read and corrected on screen. Further instructions will be sent with the proof. Hard copy proofs may be posted if no e-mail address is available. Excessive changes made by the author in the proofs, excluding typesetting errors, will be charged separately.

NEW: Online production tracking is now available for your article through Wiley-Blackwell's Author Services
Author Services enables authors to track their article - once it has been accepted - through the production process to publication online and in print. Authors can check the status of their articles online and choose to receive automated e-mails at key stages of production so they don't need to contact the production editor to check on progress. Visit for more details on online production tracking and for a wealth of resources including FAQs and tips on article preparation, submission and more.

Free access to the final PDF offprint of your article will be available through author services only. Please therefore sign up for author services if you would like to access your article PDF offprint and enjoy the many other benefits the services offers. See
Paper offprints may be ordered at prices quoted on the order form, which accompanies the proof, provided that the form is returned with the proofs. The cost is more if the order form arrives too late for the main print run. Offprints are normally dispatched within 3 weeks of publication of the issue in which the paper appears. Please contact the publishers if offprints do not arrive; however, please note that offprints are sent by surface mail, so overseas orders may take up to 6 weeks to arrive.

Author Material Archive Policy
Please note that unless specifically requested, all hardcopy or electronic material submitted will be disposed of 2 months after publication. If you require the return of any material submitted, please inform the editorial office or production editor as soon as possible.

Note to NIH Grantees
Pursuant to NIH mandate, Wiley-Blackwell will post the accepted version of contributions authored by NIH grant-holders to PubMed Central upon acceptance. This accepted version will be made publicly available 12 months after publication. For further information, see