Mitogen activated protein kinases SakAHOG1 and MpkC collaborate for Aspergillus fumigatus virulence
Ariane Cristina Mendes de Oliveira Bruder Nascimento, Thaila Fernanda dos Reis, Patrícia Alves de Castro, Juliana I. Hori, Vinícius Leite Pedro Bom, Leandro José de Assis, Leandra Naira Zambelli Ramalho, Marina Campos Rocha, Iran Malavazi, Neil Andrew Brown, Vito Valiante, Axel A. Brakhage, Daisuke Hagiwara and Gustavo H. Goldman
Article first published online: 23 MAR 2016 | DOI: 10.1111/mmi.13354
We investigated which stress responses were influenced by the MpkC and SakA mitogen-activated protein (MAP) kinases of the high-osmolarity glycerol (HOG) pathway in the fungal pathogen Aspergillus fumigatus. The ΔsakA and the double ΔmpkC ΔsakA mutants were more sensitive to osmotic and oxidative stresses, and to cell wall damaging agents. A. fumigatus ΔsakA and ΔmpkC were virulent in mouse survival experiments. In contrast, the ΔmpkC ΔsakA double mutant showed highly attenuated virulence. We propose that both Cell Wall Integrity (CWI) and HOG pathways collaborate, and that MpkC could act by modulating SakA activity upon exposure to several types of stresses and during cell wall biosynthesis.