Molecular Microbiology

Cover image for Vol. 95 Issue 3

Early View (Online Version of Record published before inclusion in an issue)

Edited By: John D. Helmann

Impact Factor: 5.026

ISI Journal Citation Reports © Ranking: 2013: 19/119 (Microbiology); 56/291 (Biochemistry & Molecular Biology)

Online ISSN: 1365-2958

Associated Title(s): Cellular Microbiology


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  1. Research Articles

    1. The phage tail tape measure protein, an inner membrane protein and a periplasmic chaperone play connected roles in the genome injection process of E. coli phage HK97

      Nichole Cumby, Kelly Reimer, Dominique Mengin-Lecreulx, Alan R. Davidson and Karen L. Maxwell

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12918

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      The phage HK97 infection process requires the presence of the bacterial inner membrane transporter protein, PtsG, and periplasmic folding chaperone, FkpA. Specific amino acid sequences in the HK97 tape measure protein encode the dependencies on these host proteins, as well as superinfection exclusion by the HK97 gp15 protein. Based on these data we propose a model for the in vivo genome injection process of HK97.

    2. You have full text access to this OnlineOpen article
      Yersinia enterocolitica type III secretion injectisomes form regularly spaced clusters, which incorporate new machines upon activation

      Mikhail Kudryashev, Andreas Diepold, Marlise Amstutz, Judith P. Armitage, Henning Stahlberg and Guy R. Cornelis

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12908

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      We show that bacterial type III secretion systems, molecular syringes that bacteria use to inject proteins into eukaryotic host cells, are arranged in small clusters on the membrane of Yersinia enterocolitica. When we activated the system by Calcium chelation, new injectisomes were formed, most of which associated next to the existing clusters. Clustering of type III secretion systems may ensure fast and efficient protein injection and thereby reduce the risk for bacteria to be detected by the host immune system.

  2. MicroCommentary

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      Last but not least: new insights into how FtsN triggers constriction during Escherichia coli cell division

      David S. Weiss

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12925

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      New findings indicate a bacterial cell division protein named FtsN triggers constriction by interacting with FtsA in the cytoplasm and the FtsQLB complex in the periplasm. The FtsN-FtsA interaction is facilitated by ZipA.

  3. Research Articles

    1. The spliceosomal PRP19 complex of trypanosomes

      Daniela L. Ambrósio, Nitika Badjatia and Arthur Günzl

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12910

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      The heteromeric PRP19 complex is important for spliceosome activation. Purification of the PRP19 orthologue in Trypanosoma brucei, a protistan parasite that processes pre-mRNA by spliced leader trans splicing, co-purified 41 [putative] spliceosomal proteins. Sedimentation analysis revealed a complex of seven subunits, consisting of the core PRP19, CDC5, PRL1 and SPF27, and, deviating from other systems, PRP17, SKIP and PPIL1, as well. The complex was predominantly associated with U2/U5/U6 snRNA spliceosomes, and SPF27 silencing interfered with the first step of splicing.

    2. You have full text access to this OnlineOpen article
      A MAT1–2 wild-type strain from Penicillium chrysogenum: functional mating-type locus characterization, genome sequencing and mating with an industrial penicillin-producing strain

      Julia Böhm, Tim A. Dahlmann, Hendrik Gümüşer and Ulrich Kück

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12909

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      We provide the first functional analysis of a MAT1-2 locus from a wild-type strain of the penicillin producing fungus Penicillium chrysogenum. The MAT1-2 locus has functions beyond sexual development and affects spore germination and light-dependent asexual sporulation. Mating of the MAT1-2 wild type with a MAT1-1 industrial producer strain generated sexual spores. We determined the genomic sequences of parental and progeny strains using next generation sequencing and found evidence for genome-wide recombination.

    3. A thioesterase bypasses the requirement for exogenous fatty acids in the plsX deletion of Streptococcus pneumoniae

      Joshua B. Parsons, Matthew W. Frank, Marc J. Eleveld, Joost Schalkwijk, Tyler C. Broussard, Marien I. de Jonge and Charles O. Rock

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12916

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      PlsX (acyl-acyl carrier protein:phosphate transacylase) is essential for phospholipid synthesis in Staphylococcus aureus, and S. aureus plsX knockouts are fatty acid auxotrophs. However, a plsX knockout in Streptococcus pneumoniae grew without a fatty acid supplement. The expression of an acyl-acyl carrier protein thioesterase (TesS) is responsible for the difference in lipid metabolism in these two bacteria.

    4. You have full text access to this OnlineOpen article
      Pivotal and distinct role for Plasmodium actin capping protein alpha during blood infection of the malaria parasite

      Markus Ganter, Zaira Rizopoulos, Herwig Schüler and Kai Matuschewski

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12922

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      Regulation of microfilament dynamics is critical for stage conversion and life cycle progression of Plasmodium and related apicomplexan parasites. Biochemical and experimental genetics evidence revealed a previously unrecognized functional separation of the two Plasmodium berghei actin capping protein (CP) subunits. Distinct actin capping activities by CP alpha homodimers during blood infection and CP alpha/beta heterodimers in mosquito stage parasites likely reflect an adaptation to different hosts. 

    5. Lineage-specific activities of a multipotent mitochondrion of trypanosomatid flagellates

      Ingrid Škodová-Sveráková, Zdeněk Verner, Tomáš Skalický, Jan Votýpka, Anton Horváth and Julius Lukeš

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12920

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      Five newly isolated trypanosomatids were tested for specific mitochondrial enzymatic activities and respiration. The collected data were mapped to phylogeny to see whether there is a correlation between mitochondrial features and phylogeny or not. We concluded that trypanosomatids, in general, possess a mitochondrion that is capable of adjusting to various environments.

    6. The BBA33 lipoprotein binds collagen and impacts Borrelia burgdorferi pathogenesis

      Hui Zhi, Eric H. Weening, Elena Magda Barbu, Jenny A. Hyde, Magnus Höök and Jon T. Skare

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12921

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      Borrelia burgdorferi, the etiologic agent of Lyme disease, binds to extracellular matrix targets, including collagen. Despite this connection, there are no borrelial proteins known to specifically mediate this interaction. In the Zhi et al. report, the surface exposed BBA33 lipoprotein is shown to bind to collagen type VI, and to a lesser extent, type IV. The absence of bba33 greatly impairs borrelial infectivity, providing a link to collagen adherence and B. burgdorferi pathogenesis.

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    8. Legionella pneumophila utilizes a single-player disulfide-bond oxidoreductase system to manage disulfide bond formation and isomerization

      Zegbeh Z. Kpadeh, Shandra R. Day, Brandy W. Mills and Paul S. Hoffman

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12914

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      A single player disulfide-bond oxidase/isomerase system from Legionella pneumophila can functionally replace the two player DsbA/DsbC system in Escherichia coli. These studies demonstrate that the system avoids the futile cycle trap by restricting the reduction of DsbA2 by two membrane spanning DsbD, thereby enabling two DsbB oxidases to recycle the oxidase. DsbA2 is required to manage disulfide bonding in cysteine-rich DotG, a critical core component of the Dot/Icm Type 4b secretion system associated with virulence.

  4. MicroReview

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      Hybrid histidine kinases in pathogenic fungi

      Tatiana A. Defosse, Anupam Sharma, Alok K. Mondal, Thomas Dugé de Bernonville, Jean-Paul Latgé, Richard Calderone, Nathalie Giglioli-Guivarc'h, Vincent Courdavault, Marc Clastre and Nicolas Papon

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12911

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      Hybrid histidine kinases (HHK) are sensing proteins widespread in the fungal kingdom. We propose here an updated compilation and classification of fungal HHK with special focus in the most prominent pathogenic fungi. This analysis revealed that a particular group of fungal HHK, namely group III HHK, remains the most conserved among fungal pathogens of human, plant and insect.

  5. Research Articles

    1. Post-transcriptional regulation of transcript abundance by a conserved member of the tristetraprolin family in Candida albicans

      Melissa L. Wells, Onica L. Washington, Stephanie N. Hicks, Clarissa J. Nobile, Nairi Hartooni, Gerald M. Wilson, Beth E. Zucconi, Weichun Huang, Leping Li, David C. Fargo and Perry J. Blackshear

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12913

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      Zfs1 in Candida albicans is a member of the tristetraprolin (TTP) family of mRNA binding and destabilizing proteins. Zfs1Δ mutants exhibited significant increases in transcripts containing one or more TTP family binding sites. Zfs1 bound to the ideal TTP binding site with high affinity, and co-immunoprecipitated with target transcripts. Although the proposed Zfs1 target transcripts were highly conserved among related species, their putative binding sites often “disappeared” with increasing evolutionary distance from the parental species.

    2. The Bacterial signal transduction protein GlnB regulates the committed step in fatty acid biosynthesis by acting as a dissociable regulatory subunit of acetyl-CoA carboxylase

      Edileusa C.M. Gerhardt, Thiago E. Rodrigues, Marcelo Müller-Santos, Fabio O. Pedrosa, Emanuel M. Souza, Karl Forchhammer and Luciano F. Huergo

      Article first published online: 30 JAN 2015 | DOI: 10.1111/mmi.12912

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      In bacteria and plant chloroplasts, the rate-limiting step in fatty acid biosynthesis is catalyzed by a multi-subunit form of the acetyl-CoA carboxylase (ACC). This enzyme carboxylates acetyl-CoA to produce malonyl-CoA, which in turn acts as the building block for fatty acid elongation. Here we show that PII proteins form a complex with ACC. This interaction results in ACC inhibition by decreasing the enzyme turnover number. Both interaction and ACC inhibition were relieved by 2-oxoglutarate.

    3. The tap-tpg gene pair on the linear plasmid functions to maintain a linear topology of the chromosome in Streptomyces rochei

      Yosi Nindita, Zhisheng Cao, Yingjie Yang, Kenji Arakawa, Yuh Shiwa, Hirofumi Yoshikawa, Michihira Tagami, Alexander Lezhava and Haruyasu Kinashi

      Article first published online: 24 JAN 2015 | DOI: 10.1111/mmi.12904

    4. Structural basis for targeting the ribosomal protein S1 of Mycobacterium tuberculosis by pyrazinamide

      Juanjuan Yang, Yindi Liu, Jing Bi, Qixu Cai, Xinli Liao, Wenqian Li, Chenyun Guo, Qian Zhang, Tianwei Lin, Yufen Zhao, Honghai Wang, Jun Liu, Xuelian Zhang and Donghai Lin

      Article first published online: 16 JAN 2015 | DOI: 10.1111/mmi.12892

    5. Identification of the amino acids essential for LytSR-mediated signal transduction in Staphylococcus aureus and their roles in biofilm-specific gene expression

      McKenzie K. Lehman, Jeffrey L. Bose, Batu K. Sharma-Kuinkel, Derek E. Moormeier, Jennifer L. Endres, Marat R. Sadykov, Indranil Biswas and Kenneth W. Bayles

      Article first published online: 16 JAN 2015 | DOI: 10.1111/mmi.12902

    6. The disruption of prenylation leads to pleiotropic rearrangements in cellular behavior in Staphylococcus aureus

      Christina N. Krute, Ronan K. Carroll, Frances E. Rivera, Andy Weiss, Ryan M. Young, Andrew Shilling, Mohsen Botlani, Sameer Varma, Bill J. Baker and Lindsey N. Shaw

      Article first published online: 16 JAN 2015 | DOI: 10.1111/mmi.12900

    7. Multiple histidines in the periplasmic domain of the Salmonella enterica sensor kinase SsrA enhance signaling in response to extracellular acidification

      David T. Mulder, Joseph B. McPhee, Sarah A. Reid-Yu, Peter J. Stogios, Alexei Savchenko and Brian K. Coombes

      Article first published online: 16 JAN 2015 | DOI: 10.1111/mmi.12895

    8. The metabolic regulator CodY links Listeria monocytogenes metabolism to virulence by directly activating the virulence regulatory gene prfA

      Lior Lobel, Nadejda Sigal, Ilya Borovok, Boris R. Belitsky, Abraham L. Sonenshein and Anat A. Herskovits

      Article first published online: 30 DEC 2014 | DOI: 10.1111/mmi.12890

    9. Enterococcus faecalis pCF10-encoded surface proteins PrgA, PrgB (aggregation substance) and PrgC contribute to plasmid transfer, biofilm formation and virulence

      Minny Bhatty, Melissa R. Cruz, Kristi L. Frank, Jenny A. Laverde Gomez, Fernando Andrade, Danielle A. Garsin, Gary M. Dunny, Heidi B. Kaplan and Peter J. Christie

      Article first published online: 30 DEC 2014 | DOI: 10.1111/mmi.12893

    10. Identification of NoxD/Pro41 as the homologue of the p22phox NADPH oxidase subunit in fungi

      Isabelle Lacaze, Hervé Lalucque, Ulrike Siegmund, Philippe Silar and Sylvain Brun

      Article first published online: 30 DEC 2014 | DOI: 10.1111/mmi.12876

    11. A two-component, multimeric endolysin encoded by a single gene

      Daniela Proença, Christophe Velours, Clara Leandro, Miguel Garcia, Madalena Pimentel and Carlos São-José

      Article first published online: 8 DEC 2014 | DOI: 10.1111/mmi.12857


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