Vital and dispensable roles of Plasmodium multidrug resistance transporters during blood- and mosquito-stage development
Sanna R. Rijpma, Maarten van der Velden, Takeshi Annoura, Joachim M. Matz, Sanketha Kenthirapalan, Taco W. A. Kooij, Kai Matuschewski, Geert-Jan van Gemert, Marga van de Vegte-Bolmer, Rianne Siebelink-Stoter, Wouter Graumans, Jai Ramesar, Onny Klop, Frans G. M. Russel, Robert W. Sauerwein, Chris J. Janse, Blandine M. Franke-Fayard and Jan B. Koenderink
Version of Record online: 23 MAY 2016 | DOI: 10.1111/mmi.13373
The role of MDR transporters of the ABC superfamily was evaluated in Plasmodium by targeting seven transporter genes, of which three could be successfully deleted. Loss of P. falciparum MDR2 results in reduced asexual growth, while in P. berghei this was the case for MDR3 and MDR5. Interestingly, deletion of MDR2 and MDR5 causes a reduction in oocyst and sporozoite production in both species, indicating a role for ABC transporters in transmission stages.