Plant, Cell & Environment

Cover image for Vol. 39 Issue 11

Accepted Articles (Accepted, unedited articles published online and citable. The final edited and typeset version of record will appear in future.)

Edited By: Keith Mott

Impact Factor: 6.169

ISI Journal Citation Reports © Ranking: 2015: 10/209 (Plant Sciences)

Online ISSN: 1365-3040


  1. 1 - 23
  1. Original Articles

    1. Clathrin Regulates Blue Light-Triggered Lateral Auxin Distribution and Hypocotyl Phototropism in Arabidopsis

      Ying Zhang, Qinqin Yu, Nan Jiang, Xu Yan, Chao Wang, Qingmei Wang, Jianzhong Liu, Muyuan Zhu, Sebastian Y. Bednarek, Jian Xu and Jianwei Pan

      Accepted manuscript online: 22 OCT 2016 06:00AM EST | DOI: 10.1111/pce.12854

      Brief Summary Statement

      Phototropism, an important survival mechanism in plants, is predominantly triggered by blue light (BL) in which asymmetric auxin distribution is essential. This study show that clathrin regulates BL-triggered hypocotyl phototropic bending through modulating endocytosis and lateralization of the PIN3 auxin efflux transporter and thereby auxin distribution.

    2. Tight Control of Nitrate Acquisition in a Plant Species that Evolved in an Extremely Phosphorus-Impoverished Environment

      M. Asaduzzaman Prodhan, Ricarda Jost, Mutsumi Watanabe, Rainer Hoefgen, Hans Lambers and Patrick M. Finnegan

      Accepted manuscript online: 20 OCT 2016 08:10PM EST | DOI: 10.1111/pce.12853

      Hakea prostrata (Proteaceae), naturally occurring on extremely phosphorus-impoverished soils, responds to nitrogen availability in a fundamentally differently way from that of model plants like Arabidopsis. Instead of accumulating nitrogen, H. prostrata tightly controls its nitrogen acquisition, independent of availability, maintaining a low total and organ nitrogen concentration. This reduces its phosphorus requirement for ribosomal RNA, the major leaf organic phosphorus fraction, contributing to its survival on phosphorus-impoverished soils. Our results on plant functioning in an extreme environment are likely valuable in developing phosphorus-use efficient crops, and thus of interest to many plant biologists.

  2. Special Issues

    1. Predicting stomatal responses to the environment from the optimization of photosynthetic gain and hydraulic cost

      John S. Sperry, Martin D. Venturas, William R.L. Anderegg, Maurizio Mencuccini, D. Scott Mackay, Yujie Wang and David M. Love

      Accepted manuscript online: 20 OCT 2016 05:50PM EST | DOI: 10.1111/pce.12852

      Current land surface models struggle to represent the complex and species-specific manner by which stomata respond to environmental cues, especially soil drought. This paper offers a solution to this problem by assuming the goal of stomatal regulation is to maximize photosynthetic gain minus hydraulic cost. A trait- and process-based "profit-maximizing" algorithm predicts realistic stomatal behavior in response to the gamut of environmental stimuli. This new approach to stomatal optimization theory may prove useful in large-scale modeling of responses to climate change.

  3. Original Articles

    1. A major locus involved in the formation of the radial oxygen loss barrier in adventitious roots of teosinte Zea nicaraguensis is located on the short-arm of chromosome 3

      Kohtaro Watanabe, Hirokazu Takahashi, Saori Sato, Shunsaku Nishiuchi, Fumie Omori, Al Imran Malik, Timothy David Colmer, Yoshiro Mano and Mikio Nakazono

      Accepted manuscript online: 20 OCT 2016 04:55AM EST | DOI: 10.1111/pce.12849


      Formation of a radial oxygen loss (ROL) barrier in roots of waterlogging-tolerant plants can enhance the longitudinal diffusion of oxygen via aerenchyma to the root tip and impede the entry of soil phytotoxins. Zea nicaraguensis, a waterlogging-tolerant wild relative of maize (Z. mays ssp. mays), forms a tight ROL barrier in its roots when waterlogged, whereas maize does not. Using Z. nicaraguensis chromosome segment introgression lines in maize, we identified that the short-arm of chromosome 3 of Z. nicaraguensis endows the inducible ROL barrier root trait. Lines with this chromosomal region had restricted ROL and reduced penetration of an apoplastic solute in the basal root zones but these functional changes were not simply related to suberin and lignin in the outer part of the roots as visualized by histochemical staining.

    2. Substantial role for carbonic anhydrase in latitudinal variation in mesophyll conductance of Populus trichocarpa Torr. & Gray

      Mina Momayyezi and Robert D. Guy

      Accepted manuscript online: 20 OCT 2016 02:25AM EST | DOI: 10.1111/pce.12851

      Using chlorophyll fluorescence and isotope discrimination methods we demonstrate that higher photosynthetic rates in black cottonwood accessions originating from higher latitude are partially supported by higher mesophyll conductance. High latitude genotypes had higher carbonic anhydrase activity and showed less reduction in chloroplast CO2 concentrations when treated with acetazolamide, an inhibitor of carbonic anhydrase. Natural and inhibitor-induced variation in carbonic anhydrase activity were similarly well correlated with mesophyll conductance.

    3. Integrated analysis of rice transcriptomic and metabolomic responses to elevated night temperatures identifies sensitivity- and tolerance-related profiles

      Ulrike Glaubitz, Xia Li, Sandra Schaedel, Alexander Erban, Ronan Sulpice, Joachim Kopka, Dirk K. Hincha and Ellen Zuther

      Accepted manuscript online: 20 OCT 2016 01:35AM EST | DOI: 10.1111/pce.12850


      “Integrated analysis of rice transcriptomic and metabolomic responses to elevated night temperatures identifies sensitivity- and tolerance-related profiles“ by Ulrike Glaubitz, Xia Li, Sandra Schaedel, Alexander Erban, Ronan Sulpice, Joachim Kopka, Dirk K. Hincha and Ellen Zuther.

      Transcript and metabolite profiling were performed on leaves from six rice cultivars in the vegetative stage under high night temperature (HNT) condition. Common HNT responsive genes in all cultivars as well as specific responses for differently tolerant cultivars were identified. Metabolite profiling and enzyme activity measurements supported the hypothesis of a highly activated TCA cycle under HNT conditions in sensitive cultivars. An integrated analysis of transcript and metabolite data enabled the identification of one sensitivity- and one tolerance-related profile pointing to an involvement of GABA signaling in sensitive and of jasmonate signaling in tolerant cultivars.

  4. Invited Reviews

    1. Water transport and energy

      Wieland Fricke

      Accepted manuscript online: 18 OCT 2016 06:40PM EST | DOI: 10.1111/pce.12848

      Water flows at the plant and cell level are set in relation to the energy required to maintain these flows. The focus is on long-distance transport (xylem, phloem) and cellular transport. While the contribution of an active water-cotransport mechanism to xylem water flow and regulation of cell water content cannot be excluded on theoretical grounds, it has to be questioned based on the energy required to support such a transport due to the high hydraulic conductivity of the plasma membrane. Long-distance transport of water, including transport along the phloem, comes cheap in energetic terms when compared to water transport at cellular level, as the latter requires a significant portion of cellular proton pump activity and respiration to support solute transport driving water movement osmotically.

  5. Original Articles

    1. The host plant Pinus pinaster exerts specific effects on phosphate efflux and polyphosphate metabolism of the ectomycorrhizal fungus Hebeloma cylindrosporum: a radiotracer, cytological staining and 31P NMR spectroscopy study

      Margarita Torres-Aquino, Adeline Becquer, Christine Le Guernevé, Julien Louche, Laurie K Amenc, Siobhan Staunton, Hervé Quiquampoix and Claude Plassard

      Accepted manuscript online: 14 OCT 2016 07:50PM EST | DOI: 10.1111/pce.12847

      In this work we examined whether a host or a non-host ectomycorrhizal (ECM) plant can enhance P efflux and affect polyphosphate (polyP) metabolism of an ECM fungus. In a system simulating the symbiotic interface in vitro, we used 32P to quantify P fluxes and 31P NMR to follow the fate of fungal polyP. For the first time, this paper shows that an ECM host plant has a direct effect on polyP hydrolysis to promote fungal Pi release.

  6. Invited Reviews

    1. Water potential regulation, stomatal behaviour and hydraulic transport under drought: deconstructing the iso/anisohydric concept

      Jordi Martínez-Vilalta and Núria Garcia-Forner

      Accepted manuscript online: 14 OCT 2016 07:15AM EST | DOI: 10.1111/pce.12846

      The relationship between leaf water potential regulation (iso/anisohydry) and stomatal behaviour is one of the foundations of our current understanding of plant water relations and drought responses. There are reasons, however, to expect that water potential regulation and stomatal behaviour may be (at least partially) uncoupled across species. We review the literature and provide a quantitative synthesis showing that species with a tight regulation of leaf water potential do not necessarily show greater stomatal control or more constrained assimilation during drought. Therefore, iso/anisohydry cannot be used as an indicator of a specific mechanism of drought-induced mortality or as a proxy for overall plant vulnerability to drought.

  7. Special Issues

    1. Leaf water stable isotopes and water transport outside the xylem

      M. M. Barbour, G. D. Farquhar and T. N. Buckley

      Accepted manuscript online: 14 OCT 2016 06:50AM EST | DOI: 10.1111/pce.12845

      Leaf water isotopes have been suggested to provide information on pathways of water movement within leaves, but interpreting measurements has been difficult. Models of spatially-explicit water transport within leaves predict sites of phase change and provide evidence of the importance of vapour phase transport. Unfortunately rigorous testing of these models is limited by measurement techniques at appropriate scales. Here we review the literature of leaf water isotopes and transport pathways and suggest that bringing these two areas together to develop a spatially, anatomically and isotopically-explicit model of leaf water transport would advance understanding in both areas by generating testable hypotheses of pools and fluxes of leaf water isotopes.

  8. Invited Reviews

    1. Carbon dioxide and water transport through plant aquaporins

      Michael Groszmann, Hannah L Osborn and John R Evans

      Accepted manuscript online: 14 OCT 2016 06:42AM EST | DOI: 10.1111/pce.12844

      Aquaporins are intrinsic membrane proteins encoded by a multigene family that function to increase membrane permeability to water, CO2 and other molecules. There are many levels of regulation (e.g. diurnal expression, membrane targeting, tetramer composition, gating) that result in highly dynamic and tissue specific control of permeability. Owing to the ease of measurement, aquaporins associated with water permeability have been more extensively characterised than those facilitating CO2 transport. Manipulation of aquaporins is actively being explored in efforts to improve plant performance with respect to plant water relations, stomatal function and photosynthesis.

  9. Special Issues

    1. A synthesis of the effects of atmospheric carbon dioxide enrichment on plant hydraulics: implications for whole-plant water use efficiency and resistance to drought

      Jean-Christophe Domec, Duncan D. Smith and Kate McCulloh

      Accepted manuscript online: 14 OCT 2016 06:11AM EST | DOI: 10.1111/pce.12843

      Here we summarize studies on the effects of elevated [CO2] (CO2e) on the structure and function of plant hydraulic architecture, and explore the implications of those changes using a model. Changes in conduit diameter and hydraulic conductance due to CO2e vary among species. The effects of CO2e on the structure and function of plant hydraulic architecture depend on the species and plant functional type. Our analysis reveals that hydraulic traits of woody plants will be negatively affected by CO2e, and those of non-woody plants will be positively affected with higher resistance to drought under future conditions.

  10. Original Articles

    1. Direct observation and modelling of embolism spread between xylem conduits: a case study in Scots pine.

      José M. Torres-Ruiz, Hervé Cochard, Maurizio Mencuccini, Sylvain Delzon and Eric Badel

      Accepted manuscript online: 14 OCT 2016 05:30AM EST | DOI: 10.1111/pce.12840

      The submitted manuscript addresses, by using X-ray microtomography and hydraulic methods, a relevant and poorly evaluated question in plant physiology: the embolism formation and spread among xylem conduits in Scots pine as a case study. Results showed a large variability in embolism resistance within a single ring despite no anatomical differences were observed in those traits associated with resistance to embolism between tracheids. Indees, and for the first time in a tracheid-based species, an increased vulnerability to embolism was observed in tracheids that were embolised once, confirming the occurrence of cavitation fatigue in conifers. Finally, a novel model was developed to simulate how embolism spreads among tracheids, allowing us to confirm the major role of the tracheid-to-tracheid air seeding in the xylem embolism spreading in Scot pine.

    2. Systems analysis of metabolism and the transcriptome in Arabidopsis thaliana roots reveals differential co-regulation upon iron, sulfur and potassium deficiency

      Ilaria Forieri, Carsten Sticht, Michael Reichelt, Norbert Gretz, Malcolm J. Hawkesford, Mario Malagoli, Markus Wirtz and Ruediger Hell

      Accepted manuscript online: 10 OCT 2016 08:51PM EST | DOI: 10.1111/pce.12842


      Acquisition of mineral nutrients by plants is of crucial importance for growth and ultimately yield of crops. In recent years the presence of a general nutrient deficiency-induced transcriptional response has been hypothesized on the basis of global transcriptome analyses from plants starved for diverse nutrients.

      By comparative investigation of iron, sulfur and potassium deficiency we provide evidence that only a small number of genes was regulated in a sense of a general nutrient-deficiency response. However, we observed nutrient specific phenotypic adaptations and specific alterations of the root phytohormone system in response to individual nutrient deficiencies. A comprehensive analysis of sulfur and iron assimilation related metabolites revealed individual patterns in response to the diverse nutrient deficiencies. Dissection of the transcriptome responses to the three nutrients showed cross-talk between the metabolism of iron and sulfur. The lack of such cross-talk under potassium deficiency underpinned the specificity of this interaction. Detailed analysis of this cross-talk revealed that the iron and sulfur uptake and metabolisation pathways were oppositely regulated upon starvation by these nutrients.

    3. Coupled Response of Stomatal and Mesophyll Conductance to Light Enhances Photosynthesis of Shade Leaves under Sunflecks

      Courtney E. Campany, Mark G. Tjoelker, Susanne von Caemmerer and Remko A. Duursma

      Accepted manuscript online: 10 OCT 2016 07:45PM EST | DOI: 10.1111/pce.12841


      Light gradients within tree canopies play a major role in the distribution of plant resources that define the photosynthetic capacity of sun and shade leaves. We found typical lower photosynthetic capacity and lower leaf nitrogen content in shade leaves of Eucalyptus trees grown in whole-tree chambers. Surprisingly, a rapid response of mesophyll and stomatal conductance to simulated sunflecks allowed shade leaves to outperform sun leaves. These dynamic physiological responses of shade leaves have implications for up-scaling leaf level measurements and understanding optimal distribution of resources in plant canopies.

    4. ERF105 is a transcription factor gene of Arabidopsis thaliana required for freezing tolerance and cold acclimation

      Sylvia Bolt, Ellen Zuther, Stefanie Zintl, Dirk K. Hincha and Thomas Schmülling

      Accepted manuscript online: 10 OCT 2016 05:00PM EST | DOI: 10.1111/pce.12838

      Low temperature is an environmental factor that adversely affects plant growth and development and limits the geographical distribution of species, it impacts also the agronomical performance of crop plants. Therefore, understanding the response to cold temperature stress is relevant for both basic biology and application. In this work, we report the charac-terization of an AP2/ERF domain-containing transcription factor gene of Arabidopsis, ERF105. ERF105 expression is induced by cold and we show that the gene is functionally important for the cold stress response. Its effect on freezing tolerance is comparable or even higher than the one of components of the well-studied CBF regulon and the expres-sion behavior of cold-responsive genes indicates that ERF105 may act upstream of the well-known CBF regulon. Taken together, we think that this is a novel and relevant contri-bution for our understanding of the response of plants to cold stress.

  11. Commentaries

    1. Turgor maintenance by osmotic adjustment, an adaptive mechanism for coping with plant water deficits.

      Neil C. Turner

      Accepted manuscript online: 10 OCT 2016 01:30PM EST | DOI: 10.1111/pce.12839

  12. Original Articles

    1. You have full text access to this OnlineOpen article
      Overexpression of PP2A-C5 that encodes the catalytic subunit 5 of protein phosphatase 2A in Arabidopsis confers better root and shoot development under salt conditions

      Rongbin Hu, Yinfeng Zhu, Jia Wei, Jian Chen, Huazhong Shi, Guoxin Shen and Hong Zhang

      Accepted manuscript online: 27 SEP 2016 07:52AM EST | DOI: 10.1111/pce.12837


      This research was to explore the role of the Arabidopsis catalytic subunit 5 of protein phosphatase 2A (i.e. PP2A-C5) in plant response to salt stresses. It provides evidence that PP2A-C5 plays a positive role in plant response to salt treatments, as the loss of function mutant pp2a-c5-1 displays more impaired growth in root and vegetative development, whereas overexpression of PP2A-C5 leads to better growth under different salt conditions including sodium chloride, potassium chloride, and sodium nitrate.

  13. Commentaries

    1. The MEP pathway as a metabolic crossroad for microbial and plant VOCs

      Manuel Rodriguez-Concepcion

      Accepted manuscript online: 16 SEP 2016 03:30AM EST | DOI: 10.1111/pce.12833

  14. Invited Reviews

    1. The functional role of xylem parenchyma cells and aquaporins during recovery from severe water stress.

      Francesca Secchi, Chiara Pagliarani and Maciej A. Zwieniecki

      Accepted manuscript online: 15 SEP 2016 02:10AM EST | DOI: 10.1111/pce.12831

      In this review we discuss role of xylem parenchyma cells (VACs) in the maintenance of xylem transport capacity and restoration of vessel/tracheid functionality following drought. We provide overview of xylem parenchyma cell biology with a special focus on aquaporins distribution and activity during the drought stress and hydraulic recovery. We complement review with presentation of the current biological model of parenchyma cell function during recovery from water stress.

  15. Original Articles

    1. OsDi19-4 acts downstream of OsCDPK14 to positively regulate ABA response in rice

      Lili Wang, Changchun Yu, Shanglin Xu, Yingguo Zhu and Wenchao Huang

      Accepted manuscript online: 14 SEP 2016 12:41PM EST | DOI: 10.1111/pce.12829

      Drought-induced 19 protein family in plants plays an important role in abiotic stress. Here we show that OsDi19-4, a member of Di19 protein family in rice, can positively regulate ABA response including ABA-induced seed germination inhibition, early seedling growth inhibition and stomatal closure. To address the question “how does OsDi19-4 work in the ABA signaling pathway”, OsCDPK14 is identified to interact with OsDi19-4 and be responsible for the phosphorylation of OsDi19-4, and the phosphorylation of OsDi19-4 is enhanced after ABA treatment. Furthermore, the increased phosphorylation of OsDi19-4 after ABA treatment can enhance its function in regulating downstream ABA-responsive genes. Our study suggests that OsDi19-4 acts downstream of OsCDPK14 to positively regulate ABA response by modulating the expression of ABA-responsive genes in rice. This work discovers a new ABA signaling pathway, which broadens our current view on the physiology functions of Di19 proteins.

    2. The Inhibition of Protein Translation Mediated by AtGCN1 is Essential for Cold Tolerance in Arabidopsis thaliana

      Linjuan Wang, Houhua Li, Chunzhao Zhao, Shengfei Li, Lingyao Kong, Wenwu Wu, Weisheng Kong, Yan Liu, Yuanyuan Wei, Jian-Kang Zhu and Hairong Zhang

      Accepted manuscript online: 31 AUG 2016 05:25AM EST | DOI: 10.1111/pce.12826

      By using a mapping approach, we cloned AtGCN1, which encodes a homologue of yeast GCN1. Our results showed that AtGCN1 directly interacts with GCN2, forming a complex that is essential for the phosphorylation of the alpha subunit of translation initiation factor eIF2 under a variety of stresses. The atgcn1 mutants were defective in the phosphorylation of eIF2α and were hypersensitive to amino acid starvation and cold stress. Ribosomal profiles showed that the translational state of mRNA was higher in the atgcn1 mutants than in the wild type under cold stress. These results indicate that eIF2α phosphorylation mediated by AtGCN1 inhibits the initiation of protein translation, which contributes to plant tolerance to cold stress.

  16. Invited Reviews

    1. Predictable "meta-mechanisms" emerge from feedbacks between transpiration and plant growth and cannot be simply deduced from short-term mechanisms

      François Tardieu and Boris Parent

      Accepted manuscript online: 29 AUG 2016 02:15AM EST | DOI: 10.1111/pce.12822

      Summary statement

      Short term mechanisms govern growth and transpiration. Because of feedbacks, their combination over time results in emerging properties ("meta-mechanisms") that may be counter-intuitive but are reproducible and consistent with results of experiments.


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