Plant, Cell & Environment

Cover image for Vol. 39 Issue 12

Accepted Articles (Accepted, unedited articles published online and citable. The final edited and typeset version of record will appear in future.)

Edited By: Keith Mott

Impact Factor: 6.169

ISI Journal Citation Reports © Ranking: 2015: 10/209 (Plant Sciences)

Online ISSN: 1365-3040


  1. 1 - 26
  1. Original Articles

    1. Solanum tuberosum ZPR1 encodes a light-regulated nuclear DNA-binding protein adjusting the circadian expression of StBBX24 to light cycle

      Agnieszka Kiełbowicz-Matuk, Jagoda Czarnecka, Ewa Banachowicz, Pascal Rey and Tadeusz Rorat

      Accepted manuscript online: 8 DEC 2016 12:30AM EST | DOI: 10.1111/pce.12875

      In this work, we report about the characterization in potato of a novel nuclear chromatin-located factor belonging to the ZPR1 family and named StZPR1. We reveal the StZPR1 ability to bind the circadian DNA cis motif ‘CAACAGCATC’, named CIRC, present in the promoter of the clock-controlled StBBX24 gene. We found that the clock-controlled expression pattern of StBBX24 is affected and delayed by 4 h towards night in transgenic lines silenced for StZPR1 expression. Importantly, other BBX genes exhibit altered circadian regulation in these lines. We conclude that ZPR1 is a novel clock-associated protein in plants necessary for the accurate rhythmic expression of specific circadian-regulated genes.

    2. Dissecting the Photoprotective Mechanism Encoded by the flv4-2 Operon: a Distinct Contribution of Sll0218 in Photosystem II Stabilization

      Luca Bersanini, Yagut Allahverdiyeva, Natalia Battchikova, Steffen Heinz, Maija Lespinasse, Essi Ruohisto, Henna Mustila, Jörg Nickelsen, Imre Vass and Eva-Mari Aro

      Accepted manuscript online: 7 DEC 2016 09:31PM EST | DOI: 10.1111/pce.12872

      This work clarifies and dissects the roles of the flv4-2 operon-encoded proteins, Flv2/Flv4 heterodimer and the elusive Sll0218, in photoprotection of the photosynthetic apparatus in Synechosystis. While Flv2/Flv4 heterodimer is involved in an alternative electron transfer route, the Sll0218 protein is localized to specific cell compartments where photosynthetic complexes are assembled, and it is involved in the stabilization of Photosystem II complexes.

    3. Herbivore perception decreases photosynthetic carbon-assimilation and reduces stomatal conductance by engaging 12-oxo-phytodienoic acid, mitogen-activated protein kinase 4 and cytokinin perception

      Ivan D. Meza-Canales, Stefan Meldau, Jorge A. Zavala and Ian T. Baldwin

      Accepted manuscript online: 7 DEC 2016 07:46AM EST | DOI: 10.1111/pce.12874

      Changes in photosynthesis by attack from herbivores have been extensively documented however the complexity of its regulation remains largely unknown. Using a functional genetics approach, we demonstrate that photosynthetic carbon assimilation and stomatal responses respond specifically to Manduca sexta elicitors by engaging 12-OPDA, MPK4-ABA and CK signaling in attacked and un-attacked leaves of Nicotiana attenuata plants.

    4. Leaf gas films contribute to rice (Oryza sativa) submergence tolerance during saline floods

      Max Herzog, Dennis Konnerup, Ole Pedersen, Anders Winkel and Timothy David Colmer

      Accepted manuscript online: 7 DEC 2016 07:43AM EST | DOI: 10.1111/pce.12873

      Flooding has detrimental effects on rice (Oryza sativa) yields, especially if flood waters are saline. We investigated the importance of leaf gas films (air layers forming around submerged super hydrophobic leaves) during rice saline submergence. We found that leaf gas film removal affected rice submergence tolerance negatively, but that Na+ and Cl could still entered into leaves, suggesting a leaf-water interface in spite of leaf gas film presence.

  2. Reviews

    1. Estimating the sensitivity of stomatal conductance to photosynthesis: A review

      Grace L. Miner, William L. Bauerle and Dennis D. Baldocchi

      Accepted manuscript online: 6 DEC 2016 11:39PM EST | DOI: 10.1111/pce.12871

      A common approach for estimating fluxes of CO2 and water in leaf and canopy models is to couple a biochemical model of photosynthesis to a semi-empirical model of stomatal conductance, such as the widely validated Ball-Berry model (e.g., Ball et al., 1987). The designated value of the slope parameter (m) in the Ball-Berry model influences transpiration estimates, but there is a lack of consensus regarding how m varies among species or plant function types (PFTs) or in response to growth conditions, and literature values are highly variable. This review explores the techniques utilized to collect m, discusses factors that can influence estimates, and compiles and synthesizes the reported values of m by species, PFT, and growth conditions for the Ball-Berry, Ball-Berry-Leuning, and the Unified Stomatal Optimization models.

  3. Original Articles

    1. Impairing both HMA4 homeologs is required for cadmium reduction in tobacco

      Verena Liedschulte, Hélène Laparra, James Nicolas Duncan Battey, Joanne Deborah Schwaar, Hervé Broye, Régis Mark, Markus Klein, Simon Goepfert and Lucien Bovet

      Accepted manuscript online: 23 NOV 2016 11:30AM EST | DOI: 10.1111/pce.12870

      Summary Statement

      HMA4.1 and HMA4.2 are key players for tobacco zinc and cadmium translocation to the shoot. A mutant approach showed that one single functional HMA4 allele is sufficient for maintaining leaf cadmium level. Therefore, the strategy is to identify optimal mutation combinations in the two HMA4 genes to reduce cadmium without impacting plant growth, i.e. homozygous combination of alleles harbouring one nonsense mutation coupled with one selected missense mutation.

    2. Rising CO2 from historical concentrations enhances the physiological performance of Brassica napus seedlings under optimal water supply but not under reduced water availability.

      Michele Faralli, Ivan G. Grove, Martin C. Hare, Peter S. Kettlewell and Fabio Fiorani

      Accepted manuscript online: 18 NOV 2016 05:50AM EST | DOI: 10.1111/pce.12868

      The productivity of many important crops is significantly threatened by water shortage and the elevated atmospheric CO2 can significantly interact with physiological processes and crop responses to drought. In this work we show that, as expected, increasing CO2 level positively modulates leaf photosynthetic traits, leaf water-use efficiency and growth under non-stressed conditions. However, the predicted elevated CO2 concentration does not reduce total evapotranspiration under drought when compared to the present (400 ppm) and historical (300 ppm) concentrations because of a larger leaf area that does not buffer transpiration. Therefore the physiological traits analysed decreased similarly under stress for all CO2 concentrations suggesting that increasing atmospheric CO2 may not significantly counteract the negative effect of increasing drought intensity on Brassica napus performance.

  4. Invited Reviews

    1. Ferns, mosses, and liverworts as model systems for light-mediated chloroplast movements

      Noriyuki Suetsugu, Takeshi Higa and Masamitsu Wada

      Accepted manuscript online: 17 NOV 2016 07:51AM EST | DOI: 10.1111/pce.12867

      Molecular mechanisms of chloroplast photorelocation movement are partially elucidated from the molecular genetic research using Arabidopsis thaliana. However, researches using fern, moss, and liverwort not only complement the results by Arabidopsis researches, but also provide new insights that never are given by Arabidopsis research.

  5. Original Articles

    1. You have full text access to this OnlineOpen article
      An empirical method that separates irreversible stem radial growth from bark water content changes in trees: theory and case studies

      Maurizio Mencuccini, Yann Salmon, Patrick Mitchell, Teemu Hölttä, Brendan Choat, Patrick Meir, Anthony O'Grady, David Tissue, Roman Zweifel, Sanna Sevanto and Sebastian Pfautsch

      Accepted manuscript online: 12 NOV 2016 02:55AM EST | DOI: 10.1111/pce.12863


      Our knowledge of the daily patterns of tree stem growth is limited. Sap flow sensors and stem dendrometers were used to isolate irreversible stem radial growth. New theory was developed that fully permits to separate elastic from irreversible diameter changes. Under drought conditions, growth was limited to night-time. Under well-watered conditions, growth tended to occur throughout the 24-hour cycle, with differences from case study to case study. This article is protected by copyright. All rights reserved.

  6. Commentaries

  7. Original Articles

    1. Transcriptomic variation among six Arabidopsis thaliana accessions identified several novel genes controlling aluminium tolerance

      Kazutaka Kusunoki, Yuki Nakano, Keisuke Tanaka, Yoichi Sakata, Hiroyuki Koyama and Yuriko Kobayashi

      Accepted manuscript online: 9 NOV 2016 06:36PM EST | DOI: 10.1111/pce.12866

      Brief Summary Statement

      Aluminum (Al) tolerance is one of the most important agronomic traits for the production of crops in the acid soil regions around the world. Comparative transcriptome analyses using the six Arabidopsis accessions contrasting Al-tolerance identified the relationship between expression level polymorphism (ELP) and Al-tolerance. Five Al-tolerance genes were newly identified from the gene set with ELP associated with Al-tolerance. Differential pattern of the enrichment of promoter SNPs of the genes with ELP suggested cis- and trans- regulatory mechanisms are involved in the ELP under Al stress.

    2. Leaf hydraulic conductance and mesophyll conductance are not closely related within a single species

      Karen E Loucos, Kevin A Simonin and Margaret M Barbour

      Accepted manuscript online: 9 NOV 2016 04:21PM EST | DOI: 10.1111/pce.12865


      It is well-known that carbon and water exchange are coupled through stomata at the leaf's surface but less is known about internal regulation of these fluxes, although coordination has been suggested. Combining measurements of stable carbon and oxygen isotopes and leaf hydraulics it was shown that internal carbon and water movement are not correlated under short-term variation in irradiance and VPd in cotton. Partitioning internal transport of CO2 revealed a novel negative relationship between chloroplast membrane conductance and growth CO2 partial pressure. These results suggest independent regulation of internal carbon and water movement within leaves.

    3. Experimental evidence for negative turgor pressure in small leaf cells of Robinia pseudoacacia L versus large cells of Metasequoia glyptostroboides Hu et W.C.Cheng. 1. Evidence from pressure-volume curve analysis of dead tissue.

      Dongmei Yang, Shaoan Pan, Yiting Ding and Melvin T. Tyree

      Accepted manuscript online: 8 NOV 2016 07:50PM EST | DOI: 10.1111/pce.12861


      It is well known that water in xylem conduits is normally under negative pressure, but the concept of negative pressure in living cells (negative turgor) has rarely been addressed experimentally except in the microscopy studies of J. Oertli on plasmolysis and cytorrhysis of living leaf cells. The purpose of this study was to confirm the biomechanical model results of Ding et al. (2014 New Phytologist) by studying negative turgor in dead tissue. This paper confirms theory, that is, that the cell walls of small cells can sustain negative turgor that is -1 MPa more negative than big cells.

    4. Vulnerability to xylem embolism as a major correlate of the environmental distribution of rainforest species on a tropical island.

      Santiago Trueba, Robin Pouteau, Frederic Lens, Taylor S. Feild, Sandrine Isnard, Mark E. Olson and Sylvain Delzon

      Accepted manuscript online: 8 NOV 2016 03:40PM EST | DOI: 10.1111/pce.12859

      Increases in drought-induced tree mortality are being observed in tropical rainforests worldwide, and are also likely to affect the geographical distribution of tropical vegetation. However, the mechanisms underlying the drought vulnerability and environmental distribution of tropical species have been little studied. Trueba et al. measured vulnerability to xylem embolism, along with other easy-to-measure wood and leaf functional traits, of 13 species endemic to the tropical archipelago of New Caledonia. This study shows that xylem embolism vulnerability stands out among other functional traits as a major driver of species environmental distribution. Drought-induced xylem embolism vulnerability behaves as a physiological trait closely associated with the habitat occupation of rainforest woody species.

    5. Experimental evidence for negative turgor pressure in small leaf cells of Robinia pseudoacacia L versus large cells of Metasequoia glyptostroboides Hu et W.C.Cheng. 2. Höfler diagrams below the volume of zero turgor and the theoretical implication for pressure-volume curves of living cells.

      Dongmei Yang, Junhui Li, Yiting Ding and Melvin T. Tyree

      Accepted manuscript online: 8 NOV 2016 03:40PM EST | DOI: 10.1111/pce.12860


      It is well known that water in xylem conduits is normally under negative pressure, but the concept of negative pressure in living cells (negative turgor) has rarely been addressed experimentally except for microscope studies of J. Oertli. This study verifies negative turgor in the small palisade cells of Robinia pseudoacacia L through pressure chamber analysis, models, and quantitative anatomical studies. We demonstrate negative turgor of up to -0.7 MPa in the common range of balance pressure and water content of pressure-volume curves: 0 to 3.3 MPa balance pressure and 0 to 0.35 in relative water content loss of leaves. We conclude that negative turgor needs to be invoked in explain the water balance of leaves with small cells (< 10 µm diameter).

  8. Invited Reviews

    1. Conflicting demands on angiosperm xylem: tradeoffs among storage, transport, and biomechanics

      R. Brandon Pratt and Anna L. Jacobsen

      Accepted manuscript online: 8 NOV 2016 03:40PM EST | DOI: 10.1111/pce.12862

      Brief Summary Statement:

      The vascular system of woody plants performs multiple functions related to transport, mechanical support, and storage. No one vascular system can maximize performance of all three functions due to structural and evolutionary limitations and this gives rise to tradeoffs. Understanding these tradeoffs and their structural basis yields insight in the evolution of this ecologically and commercially important tissue.

  9. Original Articles

    1. The influence of leaf size and shape on leaf thermal dynamics: does theory hold up under natural conditions?

      A Leigh, S Sevanto, JD Close and AB Nicotra

      Accepted manuscript online: 5 NOV 2016 04:26AM EST | DOI: 10.1111/pce.12857


      Leaf thermal dynamics are strongly influenced by the two-dimensional size and shape of leaves through boundary layer effects, so hot environments are expected to favour selection for small, narrow or dissected leaves. Using thermal imagery of leaves under field conditions, we found that leaf dissection had no or weak effects on leaf thermal dynamics, but effective leaf width strongly predicted both the cooling time constant and leaf-to-air temperature difference. Leaf area influenced the temperature range across the laminae, apparently governed largely by structural variation within leaves. Therefore, we agree that small size has adaptive value in hot environments, but not with the idea that thermal regulation is the primary evolutionary driver of leaf dissection.

    2. METHYLENE BLUE SENSITIVITY 1 (MBS1) is required for acclimation of Arabidopsis to singlet oxygen and acts downstream of β-cyclocitral

      Leonard Shumbe, Stefano D'Alessandro, Ning Shao, Anne Chevalier, Brigitte Ksas, Ralph Bock and Michel Havaux

      Accepted manuscript online: 4 NOV 2016 12:26AM EST | DOI: 10.1111/pce.12856

      In this work, we show that the MBS1 gene (METHYLENE BLUE SENSITIVITY 1) is required for the tolerance of the 1O2-overproducing Arabidopsis mutant ch1 to photooxidative stress: the ch1 mbs1 double mutant lost its capacity to acclimate to 1O2. Moreover, introduction of the mbs1 mutation in the ch1 genetic background resulted in a marked growth phenotype in low light, indicating an additional role for MBS1 in the regulation of 1O2 signaling which controls growth and development. Exposing Arabidopsis plants to high light or to the signal molecule β-cyclocitral induced accumulation of the MBS1 protein. Mutational suppression of MBS1 inhibited the effects of β-cyclocitral on gene expressions and the associated phototolerance. We have thus identified the first downstream partner in the β-cyclocitral-mediated 1O2 signaling pathway in plants.

    3. Temperature-compensated cell production rate and elongation zone length in the root of Arabidopsis thaliana

      Xiaoli Yang, Gang Dong, K. Palaniappan, Guohua Mi and Tobias I. Baskin

      Accepted manuscript online: 3 NOV 2016 11:22PM EST | DOI: 10.1111/pce.12855

      How temperature affects growth components in roots has been little studied. Based on new software for determining the velocity profile, we present a kinematic analysis of growth parameters for the Arabidopsis thaliana root for seedlings grown continuously at temperatures ranging from 15 to 30˚C. A notable finding is that, between 15 to 25˚C, the cumulative rate of cell production is temperature invariant, implying the existence of hitherto unsuspected mechanisms for temperature compensation.

  10. Special Issues

    1. Predicting stomatal responses to the environment from the optimization of photosynthetic gain and hydraulic cost

      John S. Sperry, Martin D. Venturas, William R.L. Anderegg, Maurizio Mencuccini, D. Scott Mackay, Yujie Wang and David M. Love

      Accepted manuscript online: 20 OCT 2016 05:50PM EST | DOI: 10.1111/pce.12852

      Current land surface models struggle to represent the complex and species-specific manner by which stomata respond to environmental cues, especially soil drought. This paper offers a solution to this problem by assuming the goal of stomatal regulation is to maximize photosynthetic gain minus hydraulic cost. A trait- and process-based "profit-maximizing" algorithm predicts realistic stomatal behavior in response to the gamut of environmental stimuli. This new approach to stomatal optimization theory may prove useful in large-scale modeling of responses to climate change.

  11. Original Articles

    1. A major locus involved in the formation of the radial oxygen loss barrier in adventitious roots of teosinte Zea nicaraguensis is located on the short-arm of chromosome 3

      Kohtaro Watanabe, Hirokazu Takahashi, Saori Sato, Shunsaku Nishiuchi, Fumie Omori, Al Imran Malik, Timothy David Colmer, Yoshiro Mano and Mikio Nakazono

      Accepted manuscript online: 20 OCT 2016 04:55AM EST | DOI: 10.1111/pce.12849


      Formation of a radial oxygen loss (ROL) barrier in roots of waterlogging-tolerant plants can enhance the longitudinal diffusion of oxygen via aerenchyma to the root tip and impede the entry of soil phytotoxins. Zea nicaraguensis, a waterlogging-tolerant wild relative of maize (Z. mays ssp. mays), forms a tight ROL barrier in its roots when waterlogged, whereas maize does not. Using Z. nicaraguensis chromosome segment introgression lines in maize, we identified that the short-arm of chromosome 3 of Z. nicaraguensis endows the inducible ROL barrier root trait. Lines with this chromosomal region had restricted ROL and reduced penetration of an apoplastic solute in the basal root zones but these functional changes were not simply related to suberin and lignin in the outer part of the roots as visualized by histochemical staining.

    2. The host plant Pinus pinaster exerts specific effects on phosphate efflux and polyphosphate metabolism of the ectomycorrhizal fungus Hebeloma cylindrosporum: a radiotracer, cytological staining and 31P NMR spectroscopy study

      Margarita Torres-Aquino, Adeline Becquer, Christine Le Guernevé, Julien Louche, Laurie K Amenc, Siobhan Staunton, Hervé Quiquampoix and Claude Plassard

      Accepted manuscript online: 14 OCT 2016 07:50PM EST | DOI: 10.1111/pce.12847

      In this work we examined whether a host or a non-host ectomycorrhizal (ECM) plant can enhance P efflux and affect polyphosphate (polyP) metabolism of an ECM fungus. In a system simulating the symbiotic interface in vitro, we used 32P to quantify P fluxes and 31P NMR to follow the fate of fungal polyP. For the first time, this paper shows that an ECM host plant has a direct effect on polyP hydrolysis to promote fungal Pi release.

  12. Invited Reviews

    1. Water potential regulation, stomatal behaviour and hydraulic transport under drought: deconstructing the iso/anisohydric concept

      Jordi Martínez-Vilalta and Núria Garcia-Forner

      Accepted manuscript online: 14 OCT 2016 07:15AM EST | DOI: 10.1111/pce.12846

      The relationship between leaf water potential regulation (iso/anisohydry) and stomatal behaviour is one of the foundations of our current understanding of plant water relations and drought responses. There are reasons, however, to expect that water potential regulation and stomatal behaviour may be (at least partially) uncoupled across species. We review the literature and provide a quantitative synthesis showing that species with a tight regulation of leaf water potential do not necessarily show greater stomatal control or more constrained assimilation during drought. Therefore, iso/anisohydry cannot be used as an indicator of a specific mechanism of drought-induced mortality or as a proxy for overall plant vulnerability to drought.

  13. Special Issues

    1. Leaf water stable isotopes and water transport outside the xylem

      M. M. Barbour, G. D. Farquhar and T. N. Buckley

      Accepted manuscript online: 14 OCT 2016 06:50AM EST | DOI: 10.1111/pce.12845

      Leaf water isotopes have been suggested to provide information on pathways of water movement within leaves, but interpreting measurements has been difficult. Models of spatially-explicit water transport within leaves predict sites of phase change and provide evidence of the importance of vapour phase transport. Unfortunately rigorous testing of these models is limited by measurement techniques at appropriate scales. Here we review the literature of leaf water isotopes and transport pathways and suggest that bringing these two areas together to develop a spatially, anatomically and isotopically-explicit model of leaf water transport would advance understanding in both areas by generating testable hypotheses of pools and fluxes of leaf water isotopes.

    2. A synthesis of the effects of atmospheric carbon dioxide enrichment on plant hydraulics: implications for whole-plant water use efficiency and resistance to drought

      Jean-Christophe Domec, Duncan D. Smith and Kate McCulloh

      Accepted manuscript online: 14 OCT 2016 06:11AM EST | DOI: 10.1111/pce.12843

      Here we summarize studies on the effects of elevated [CO2] (CO2e) on the structure and function of plant hydraulic architecture, and explore the implications of those changes using a model. Changes in conduit diameter and hydraulic conductance due to CO2e vary among species. The effects of CO2e on the structure and function of plant hydraulic architecture depend on the species and plant functional type. Our analysis reveals that hydraulic traits of woody plants will be negatively affected by CO2e, and those of non-woody plants will be positively affected with higher resistance to drought under future conditions.


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