Impairing both HMA4 homeologs is required for cadmium reduction in tobacco
Verena Liedschulte, Hélène Laparra, James Nicolas Duncan Battey, Joanne Deborah Schwaar, Hervé Broye, Régis Mark, Markus Klein, Simon Goepfert and Lucien Bovet
Accepted manuscript online: 23 NOV 2016 11:30AM EST | DOI: 10.1111/pce.12870
HMA4.1 and HMA4.2 are key players for tobacco zinc and cadmium translocation to the shoot. A mutant approach showed that one single functional HMA4 allele is sufficient for maintaining leaf cadmium level. Therefore, the strategy is to identify optimal mutation combinations in the two HMA4 genes to reduce cadmium without impacting plant growth, i.e. homozygous combination of alleles harbouring one nonsense mutation coupled with one selected missense mutation.