Plant, Cell & Environment

Cover image for Vol. 39 Issue 10

Accepted Articles (Accepted, unedited articles published online and citable. The final edited and typeset version of record will appear in future.)

Edited By: Keith Mott

Impact Factor: 6.169

ISI Journal Citation Reports © Ranking: 2015: 10/209 (Plant Sciences)

Online ISSN: 1365-3040


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  1. Original Articles

    1. Overexpression of PP2A-C5 that encodes the catalytic subunit 5 of protein phosphatase 2A in Arabidopsis confers better root and shoot development under salt conditions

      Rongbin Hu, Yinfeng Zhu, Jia Wei, Jian Chen, Huazhong Shi, Guoxin Shen and Hong Zhang

      Accepted manuscript online: 27 SEP 2016 07:52AM EST | DOI: 10.1111/pce.12837


      This research was to explore the role of the Arabidopsis catalytic subunit 5 of protein phosphatase 2A (i.e. PP2A-C5) in plant response to salt stresses. It provides evidence that PP2A-C5 plays a positive role in plant response to salt treatments, as the loss of function mutant pp2a-c5-1 displays more impaired growth in root and vegetative development, whereas overexpression of PP2A-C5 leads to better growth under different salt conditions including sodium chloride, potassium chloride, and sodium nitrate.

    2. Subcellular reprogramming of metabolism during cold acclimation in Arabidopsis thaliana

      Imke I. Hoermiller, Thomas Naegele, Hanna Augustin, Simon Stutz, Wolfram Weckwerth and Arnd G. Heyer

      Accepted manuscript online: 19 SEP 2016 03:10PM EST | DOI: 10.1111/pce.12836

      Cold acclimation of plants involves not only changes in metabolite concentrations, but also of their sub-cellular compartmentalization.

      Using a combination of metabolite profiling and fractionation of cellular compartments, sub-cellular metabolite dynamics during cold acclimation were studied in Arabidopsis wildtype and mutants affected in primary metabolism. Although compartmentalization was relatively robust against temperature, characteristic changes occurred especially for the plastids and cytosol. A defect in starch metabolism had a strong impact on these dynamics and reduced plant freezing tolerance.

  2. Invited Reviews

    1. Optimal plant water economy

      Thomas N Buckley, Lawren Sack and Graham D Farquhar

      Accepted manuscript online: 19 SEP 2016 01:20PM EST | DOI: 10.1111/pce.12823

      We review the theory of optimal regulation of plant water loss and suggest directions for continuing research, including (i) improving analytical approximations to the classical theory, as current approximations ignore boundary layer and mesophyll resistance and represent the theory quite poorly; (ii) exploring the role of cyclical variations in hydraulic conductance in optimal diurnal regulation; (iii) distinguishing optimal stomatal responses from changes in stomatal conductance that emerge from optimal shifts in carbon partitioning at long time scales; and (iv) further developing the whole-plant theory and testing its predictions.

  3. Original Articles

    1. Phenotypic plasticity to altered apical bud temperature in Cucumis sativus: more leaves-smaller leaves and vice versa

      Andreas Savvides, Wim van Ieperen, Janneke A. Dieleman and Leo F.M. Marcelis

      Accepted manuscript online: 19 SEP 2016 03:30AM EST | DOI: 10.1111/pce.12835

      Brief summary statement

      In a 28-days study, we maintained temperature differences between apical bud (Tbud) and the rest of the plant (Tplant) ranging from -7 to +8 oC using a custom-made bud temperature control system. Differences between Tbud and Tplant considerably influenced vertical leaf area distribution over the shoot: increasing Tbud beyond Tplant resulted in more and smaller leaves, while decreasing Tbud below Tplant resulted in less and larger leaves. The trade-off between leaf number and leaf area resulted in phenotypic alterations that cannot be predicted, e.g. by crop models, when assuming plant temperature uniformity.

  4. Commentaries

    1. The MEP pathway as a metabolic crossroad for microbial and plant VOCs

      Manuel Rodriguez-Concepcion

      Accepted manuscript online: 16 SEP 2016 03:30AM EST | DOI: 10.1111/pce.12833

  5. Original Articles

    1. C4 photosynthesis in C3 rice: a theoretical analysis of biochemical and anatomical factors

      Shuyue Wang, Danny Tholen and Xin-Guang Zhu

      Accepted manuscript online: 15 SEP 2016 02:56AM EST | DOI: 10.1111/pce.12834

      A 3D two-cell reaction diffusion model was developed to explore the photosynthetic efficiency of the C4 metabolic cycle in the anatomical and biochemical background of a C3 leaf. Our results show that integrating a C4 metabolic pathway into rice leaves may lead to an improved photosynthesis under current ambient CO2 concentrations. Partitioning of energy between C3 and C4 photosynthesis and the partitioning of Rubisco between mesophyll and bundle-sheath cells are decisive factors controlling photosynthetic efficiency in an engineered C3-C4 leaf.

  6. Invited Reviews

    1. The functional role of xylem parenchyma cells and aquaporins during recovery from severe water stress.

      Francesca Secchi, Chiara Pagliarani and Maciej A. Zwieniecki

      Accepted manuscript online: 15 SEP 2016 02:10AM EST | DOI: 10.1111/pce.12831

      In this review we discuss role of xylem parenchyma cells (VACs) in the maintenance of xylem transport capacity and restoration of vessel/tracheid functionality following drought. We provide overview of xylem parenchyma cell biology with a special focus on aquaporins distribution and activity during the drought stress and hydraulic recovery. We complement review with presentation of the current biological model of parenchyma cell function during recovery from water stress.

  7. Original Articles

    1. Petunia hybrida PDR2 is Involved in Herbivore Defense by Controlling Steroidal Contents in Trichomes

      Joëlle Sasse, Markus Schlegel, Lorenzo Borghi, Friederike Ullrich, Miyoung Lee, Guo-Wei Liu, José-Luis Giner, Oliver Kayser, Laurent Bigler, Enrico Martinoia and Tobias Kretzschmar

      Accepted manuscript online: 15 SEP 2016 02:10AM EST | DOI: 10.1111/pce.12828

      Plants fight against insect herbivory by producing deterrent and toxic compounds. Most of these compounds are stored at exposed sites such as leaf borders and trichomes. Here we show that the Petunia ABC transporter PDR2 plays an important role in deposition of petuniolides and petuniasterones, natural insecticides produced by this plant and that plants with impaired PDR2 function are preferentially attacked by insects.

    2. OsDi19-4 acts downstream of OsCDPK14 to positively regulate ABA response in rice

      Lili Wang, Changchun Yu, Shanglin Xu, Yingguo Zhu and Wenchao Huang

      Accepted manuscript online: 14 SEP 2016 12:41PM EST | DOI: 10.1111/pce.12829

      Drought-induced 19 protein family in plants plays an important role in abiotic stress. Here we show that OsDi19-4, a member of Di19 protein family in rice, can positively regulate ABA response including ABA-induced seed germination inhibition, early seedling growth inhibition and stomatal closure. To address the question “how does OsDi19-4 work in the ABA signaling pathway”, OsCDPK14 is identified to interact with OsDi19-4 and be responsible for the phosphorylation of OsDi19-4, and the phosphorylation of OsDi19-4 is enhanced after ABA treatment. Furthermore, the increased phosphorylation of OsDi19-4 after ABA treatment can enhance its function in regulating downstream ABA-responsive genes. Our study suggests that OsDi19-4 acts downstream of OsCDPK14 to positively regulate ABA response by modulating the expression of ABA-responsive genes in rice. This work discovers a new ABA signaling pathway, which broadens our current view on the physiology functions of Di19 proteins.

  8. Special Issues

    1. Non-selective cation channel activity of aquaporin AtPIP2;1 regulated by Ca2+ and pH

      Caitlin S Byrt, Manchun Zhao, Mohamad Kourghi, Jayakumar Bose, Sam W. Henderson, Jiaen Qiu, Matthew Gilliham, Carolyn Schultz, Manuel Schwarz, Sunita A Ramesh, Andrea Yool and Steve Tyerman

      Accepted manuscript online: 13 SEP 2016 03:15AM EST | DOI: 10.1111/pce.12832

      The paradigm that aquaporins may only allow permeation of neutral solutes has been challenged by examples of some animal aquaporins that can act as ion channels. Here we reveal that AtPIP2;1 is permeable to both water and Na+ in heterologous systems. The AtPIP2;1 ionic conductance is inhibited by low pH and Ca2+ similar to previous observations for non-selective cation channels indicating that AtPIP2;1 is a candidate for facilitating Na+ flux across the plasma membrane of root cells and other cells that express PIP2;1, such as guard cells.

    2. Association between water and carbon dioxide transport in leaf plasma membranes: assessing the role of aquaporins.

      Manchun Zhao, Hwei-Ting Tan, Johannes Scharwies, Kara Levin, John R Evans and Stephen D Tyerman

      Accepted manuscript online: 13 SEP 2016 02:45AM EST | DOI: 10.1111/pce.12830

      Short Summary:

      The osmotic permeability to water and diffusive permeability to CO2 of plasma membranes isolated from leaves were examined to assess co-regulation of transport through aquaporins. The diffusive permeability to CO2 was not limited by unstirred layers or limitations from carbonic anhydrase used in the method. While showing very different osmotic water permeability, plasma membranes from pea and Arabidopsis showed similar CO2 permeability. The temperature dependence of water and CO2 permeation was measured indicating involvement of aquaporins, however, the inhibition of aquaporins reduced water permeability but did not change CO2 permeability. The two permeabilities were positively correlated but only weakly so. These observations are relevant to understanding the role of aquaporins in mesophyll conductance to CO2, and the proposed co-regulation of water and CO2 transport in leaves.

  9. Commentaries

    1. Time to grow: Factors that control plant growth during mild to moderate drought stress.

      Paul E. Verslues

      Accepted manuscript online: 2 SEP 2016 08:15AM EST | DOI: 10.1111/pce.12827

  10. Original Articles

    1. The Inhibition of Protein Translation Mediated by AtGCN1 is Essential for Cold Tolerance in Arabidopsis thaliana

      Linjuan Wang, Houhua Li, Chunzhao Zhao, Shengfei Li, Lingyao Kong, Wenwu Wu, Weisheng Kong, Yan Liu, Yuanyuan Wei, Jian-Kang Zhu and Hairong Zhang

      Accepted manuscript online: 31 AUG 2016 05:25AM EST | DOI: 10.1111/pce.12826

      By using a mapping approach, we cloned AtGCN1, which encodes a homologue of yeast GCN1. Our results showed that AtGCN1 directly interacts with GCN2, forming a complex that is essential for the phosphorylation of the alpha subunit of translation initiation factor eIF2 under a variety of stresses. The atgcn1 mutants were defective in the phosphorylation of eIF2α and were hypersensitive to amino acid starvation and cold stress. Ribosomal profiles showed that the translational state of mRNA was higher in the atgcn1 mutants than in the wild type under cold stress. These results indicate that eIF2α phosphorylation mediated by AtGCN1 inhibits the initiation of protein translation, which contributes to plant tolerance to cold stress.

    2. Nitrogen fertilization and δ18O of CO2 have no effect on 18O-enrichment of leaf water and cellulose in Cleistogenes squarrosa (C4) – is VPD the sole control?

      Hai Tao Liu, Xiao Ying Gong, Rudi Schäufele, Fang Yang, Regina Theresia Hirl, Anja Schmidt and Hans Schnyder

      Accepted manuscript online: 31 AUG 2016 03:25AM EST | DOI: 10.1111/pce.12824

      The 18O enrichment of cellulose stores physiological information from 18O enrichment of leaf water, thus is of great interest to a range of scientific disciplines including palaeoecology, global change science, and plant physiology. However, the propagation of the 18O signal from leaf water to cellulose is associated with some degree of signal attenuation (termed pexpx) that is not well understood. Here we show that the attenuation responds to VPD in a C4 grass (Cleistogenes squarrosa), but was not influenced by nitrogen nutrition or the oxygen isotope composition of CO2.

    3. Segregation of Nitrogen use Between Ammonium and Nitrate of Ectomycorrhizas and Beech Trees

      Martin Leberecht, Michael Dannenmann, Javier Tejedor, Judy Simon, Heinz Rennenberg and Andrea Polle

      Accepted manuscript online: 29 AUG 2016 02:25AM EST | DOI: 10.1111/pce.12820

      Ammonium (NH4+) and nitrate (NO3-) uptake of beech (Fagus sylvatica) and their associated ectomycorrhizal (EM) assemblages were studied by 15 N labelling under field conditions.

      In native EM assemblages, long- and short-term 15 N uptake from NH4+ was higher than that from NO3-, regardless of season, water availability and site exposure, whereas in beech long-term 15 N uptake from NO3- was higher than from NH4 + .

      The transfer rates from the EM to beech were lower for 15 N from NH4+ than from NO3-.15 N content in EM was correlated with 15NH4 + -, but not with 15NO3--derived N uptake of the plant suggesting stronger control of the EM assemblage on N provision to the host from NH4+ than from NO3-.

  11. Invited Reviews

    1. Predictable "meta-mechanisms" emerge from feedbacks between transpiration and plant growth and cannot be simply deduced from short-term mechanisms

      François Tardieu and Boris Parent

      Accepted manuscript online: 29 AUG 2016 02:15AM EST | DOI: 10.1111/pce.12822

      Summary statement

      Short term mechanisms govern growth and transpiration. Because of feedbacks, their combination over time results in emerging properties ("meta-mechanisms") that may be counter-intuitive but are reproducible and consistent with results of experiments.

  12. Original Articles

    1. Hydrogen isotopic differences between C3 and C4 land plant lipids: consequences of compartmentation in C4 photosynthetic chemistry and C3 photorespiration

      Youping Zhou, Kliti Grice, Hilary Stuart-Williams, Charles H. Hocart, Arthur Gessler and *Graham D. Farquhar

      Accepted manuscript online: 27 AUG 2016 02:10AM EST | DOI: 10.1111/pce.12821


      • Analysed the 2H/1H ratio of C-Hs of controlled & field grown C3 and C4 plant leaf lipids.
      • C3 versus C4 differences in lipid-over-leaf-water 2H depletion are lipid pathway-dependent.
      • Compartmentation and photorespiration identified as the biochemical bases for such differences.
    2. Combined use of herbivore-induced plant volatiles and sex pheromones for mate location in braconid parasitoids

      Hao Xu, Gaylord Desurmont, Thomas Degen, Guoxin Zhou, Diane Laplanche, Luka Henryk and Ted Turlings

      Accepted manuscript online: 18 AUG 2016 11:01PM EST | DOI: 10.1111/pce.12818

      We demonstrate that parasitic wasps use herbivore-induced plant volatiles (HIPVs) in combination with sex pheromones to locate mates, and foraging for hosts and mates are both orientated by HIPVs in parasitoids. To our knowledge no other study has specifically addressed the use of HIPVs in mate location of parasitoids, and the inclusion of four species of wasps allows us to draw several general conclusions, but also call attention to differences among species in terms of mate searching strategies.

    3. You have full text access to this OnlineOpen article
      A User-Friendly Means to Scale from the Biochemistry of Photosynthesis to Whole Crop Canopies and Production in Time and Space – Development of Java WIMOVAC

      Qingfeng Song, Dairui Chen, Stephen P Long and Xin-Guang Zhu

      Accepted manuscript online: 16 AUG 2016 08:05AM EST | DOI: 10.1111/pce.12816

      Windows Intuitive Model of Vegetation response to Atmosphere and Climate Change (WIMOVAC) has been used widely as a generic mechanistically-rich model scaling from biochemistry of leaf photosynthesis to canopy processes and plant production. This paper describes an open-source JAVA user-friendly version of WIMOVAC. It is platform independent and can be easily downloaded to a laptop and used without any prior programming skill.

    4. Freezing behaviors in wintering Cornus florida flower bud tissues revisited using MRI

      Masaya Ishikawa, Hiroyuki Ide, Hideyuki Yamazaki, Hiroki Murakawa, Kazuyuki Kuchitsu, William S. Price and Yoji Arata

      Accepted manuscript online: 6 AUG 2016 07:39AM EST | DOI: 10.1111/pce.12813

      How plant tissues in a complex organ respond to freezing is not readily visible and the diversity of such responses (freezing behaviors) and their controlling mechanisms remain unexplored in spite of their importance in cold hardiness mechanisms. In this study, the use of high resolution MRI allowed visualization of freezing behaviors in Cornus florida flower bud tissues: only anthers and ovules remain stably supercooled to -14 – -21 °C while other floral tissues tolerate extracellular freezing. The abrupt breakdown of supercooled individual ovules and anthers resulted in their all-or-nothing type of injuries. Only gametophytic tissues remaining unfrozen is a novel freezing behavior in flower buds and the distribution of ice nucleation activity in flower buds corroborated such a freezing behavior

    5. You have full text access to this OnlineOpen article
      Time of day determines Arabidopsis transcriptome and growth dynamics under mild drought

      Marieke Dubois, Hannes Claeys, Lisa Van den Broeck and Dirk Inzé

      Accepted manuscript online: 1 AUG 2016 03:25PM EST | DOI: 10.1111/pce.12809

      Drought stress forms a major environmental constraint for agriculture worldwide. Drought triggers specific responses in plants, depending on stress severity and duration, and on the organ and its developmental stage. In young, developing leaves, growth is repressed as a mechanism to save water and energy, but the underlying molecular basis is largely unknown. Here, we present a novel approach to explore the short-term molecular mechanisms controlling leaf growth inhibition following drought and show that the time of day is a major determinant of drought responses, affecting both growth and transcriptome dynamics.

    6. Genome interrogation for novel salinity tolerant Arabidopsis mutants

      Niels van Tol, Johan Pinas, Henk Schat, Paul J.J. Hooykaas and Bert J. van der Zaal

      Accepted manuscript online: 26 JUL 2016 02:15AM EST | DOI: 10.1111/pce.12805

      We have investigated whether a capacity to withstand normally lethal levels of salinity can be evoked in the model plant species Arabidopsis thaliana by inducing large scale and more or less random distortion of gene expression patterns. To this end, we have used zinc finger artificial transcription factors (ZF-ATFs). Within a relatively small collection of Arabidopsis lines expressing ZF-ATFs we found a large number of lines that were strongly tolerant to 100 mM NaCl. We demonstrate that several ZF-ATF gene constructs act as dominant and heritable agents for salinity tolerance.

    7. Effects of different arbuscular mycorrhizal fungal backgrounds and soils on olive plants growth and water relation properties under well-watered and drought conditions

      Monica Calvo-Polanco, Iván Sánchez-Castro, Manuel Cantos, José Luis García, Rosario Azcón, Juan Manuel Ruiz-Lozano, Carmen R. Beuzón and Ricardo Aroca

      Accepted manuscript online: 23 JUL 2016 10:05AM EST | DOI: 10.1111/pce.12807

      Olive trees are exposed to drought and AM symbiosis may enhance their drought tolerance. Here AM inocula from different origin (humid or arid) were used in order to determine how AM fungi or soil origin modify the response of olive trees to drought. Also novel nine aquaporins from olive trees were cloned. It was found that the AM from the humid origin were the most effective in enhancing drought tolerance, even in the arid soil.


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