Plant, Cell & Environment

Cover image for Vol. 39 Issue 8

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Keith Mott

Impact Factor: 6.169

ISI Journal Citation Reports © Ranking: 2015: 10/209 (Plant Sciences)

Online ISSN: 1365-3040


  1. 1 - 37
  1. Original Articles

    1. Control of floral transition in the bioenergy crop switchgrass

      Lifang Niu, Chunxiang Fu, Hao Lin, Tezera W. Wolabu, Yanqi Wu, Zeng-Yu Wang and Million Tadege

      Version of Record online: 20 JUL 2016 | DOI: 10.1111/pce.12769

      Floral transition is an important biomass trait because longer vegetative phase results in the accumulation of more lignocellulosic biomass. Switchgrass is one of the dedicated biomass feedstocks in the U.S. but the control of its floral transition has not been studied at the molecular level. In this report, we identified PvFT1 as one of the switchgrass florigens together with three AP1-like downstream targets and two SOC1-like genes, which together comprise the switchgrass floral integrators similar to Arabidopsis and rice. This work lays the foundation for understanding the molecular mechanism of floral transition in switchgrass and its ultimate manipulation for improving biomass feedstock yield.

    2. Diacylglycerol kinases activate tobacco NADPH oxidase-dependent oxidative burst in response to cryptogein

      Jean-Luc Cacas, Patricia Gerbeau-Pissot, Jérôme Fromentin, Catherine Cantrel, Dominique Thomas, Emmanuelle Jeannette, Tetiana Kalachova, Sébastien Mongrand, Françoise Simon-Plas and Eric Ruelland

      Version of Record online: 20 JUL 2016 | DOI: 10.1111/pce.12771

      Cryptogein is a protein secreted by the oomycete Phytophthora cryptogea that activates defence mechanisms in tobacco. We show here in BY-2 tobacco suspension cells that phosphatidic acid rapidly accumulates in response to cryptogein because of the coordinated onset of phosphoinositide-dependent phospholipase C and diacylglycerol kinase activities. Both enzyme specific inhibitors and silencing of the phylogenetic cluster III of the tobacco DGK family were found to reduce PA production upon elicitation and to strongly decrease the RBOHD-mediated oxidative burst. This establishes that phosphatidic acid production by diacylglycerol kinases is upstream of the oxidative burst in response to cryptogein.

  2. Meeting Reports

    1. Plant hydraulics as a central hub integrating plant and ecosystem function: meeting report for ‘Emerging Frontiers in Plant Hydraulics’ (Washington, DC, May 2015)

      Lawren Sack, Marilyn C. Ball, Craig Brodersen, Stephen D. Davis, David L. Des Marais, Lisa A. Donovan, Thomas J. Givnish, Uwe G. Hacke, Travis Huxman, Steven Jansen, Anna L. Jacobsen, Daniel M. Johnson, George W. Koch, Christophe Maurel, Katherine A. McCulloh, Nate G. McDowell, Andrew McElrone, Frederick C. Meinzer, Peter J. Melcher, Gretchen North, Matteo Pellegrini, William T. Pockman, R. Brandon Pratt, Anna Sala, Louis S. Santiago, Jessica A. Savage, Christine Scoffoni, Sanna Sevanto, John Sperry, Stephen D. Tyerman, Danielle Way and N. Michele Holbrook

      Version of Record online: 20 JUL 2016 | DOI: 10.1111/pce.12732

      Plant hydraulics is increasingly recognized as a central hub relating fields within plant biology, ecology, evolution, palaeobiology and agriculture, essential to grand challenges such as anticipating and mitigating the impacts of climate change, and improving the security and sustainability of our food supply. A workshop entitled ‘Emerging Frontiers in Plant Hydraulics’ supported by the National Science Foundation, was held in Washington DC, 2015. We summarize the discussions, including controversies regarding measurements and analyses, the emerging frontiers of up-scaled and down-scaled inter-disciplinary research, and the strengthening of connections between research in plant hydraulics, that in allied fields and global modelling efforts.

  3. Original Articles

    1. Hydraulic conductance and the maintenance of water balance in flowers

      Adam B. Roddy, Craig R. Brodersen and Todd E. Dawson

      Version of Record online: 18 JUL 2016 | DOI: 10.1111/pce.12761

      Flowers are fundamental to successful reproduction for most angiosperm species, yet we know little about how they maintain turgor and regulate water supply and loss. We surveyed a phylogenetically diverse set of species for hydraulic and structural traits associated with the ability to transport water and prevent water loss. The largest variation was among major angiosperm clades, which showed divergent hydraulic structure–function relationships. This suggests that flower of more recently derived clades may rely on preventing the loss of stored water to maintain turgor rather than maintaining high fluxes of water to meet the demands of transpiration.

    2. Photoperiod-dependent changes in the phase of core clock transcripts and global transcriptional outputs at dawn and dusk in Arabidopsis

      Anna Flis, Ronan Sulpice, Daniel D. Seaton, Alexander A. Ivakov, Magda Liput, Christin Abel, Andrew J. Millar and Mark Stitt

      Version of Record online: 15 JUL 2016 | DOI: 10.1111/pce.12754

    3. Most stomatal closure in woody species under moderate drought can be explained by stomatal responses to leaf turgor

      Celia M. Rodriguez-Dominguez, Thomas N. Buckley, Gregorio Egea, Alfonso de Cires, Virginia Hernandez-Santana, Sebastia Martorell and Antonio Diaz-Espejo

      Version of Record online: 15 JUL 2016 | DOI: 10.1111/pce.12774

    4. Hd3a, RFT1 and Ehd1 integrate photoperiodic and drought stress signals to delay the floral transition in rice

      Francesca Galbiati, Remo Chiozzotto, Franca Locatelli, Alberto Spada, Annamaria Genga and Fabio Fornara

      Version of Record online: 11 JUL 2016 | DOI: 10.1111/pce.12760

      Rice plants grown in rainfed areas can experience periods of water scarcity, culminating in drought stress. How drought signals impact on developmental switches, including the transition from vegetative to reproductive growth, has never been thoroughly assessed. We set up a protocol that allows monitoring the effects of drought stress while plants are undergoing reproductive phase change, and using a genome-wide transcriptomic approach we identified clusters of genes differentially responding to drought under conditions that either promote or repress the floral transition. The emerging picture suggests that rice plants experiencing drought adopt a distinct strategy, compared to Arabidopsis, to regulate plant reproduction, and that specific genes in the flowering network act as hubs where abiotic and developmental signals converge.

    5. Systems genetics reveals key genetic elements of drought induced gene regulation in diploid potato

      Dennis van Muijen, A.M. Anithakumari, Chris Maliepaard, Richard G. F. Visser and C. Gerard van der Linden

      Version of Record online: 29 JUN 2016 | DOI: 10.1111/pce.12744

      Solanum tuberosum is a water efficient staple crop yet highly sensitive to drought. A systems genetics approach was used to identify genes and loci involved in drought response. Systems genetics analysis pinpoints to a putative Nuclear factor y subunit C4 gene as key candidate underlying a major trans eQTL hotspot specific to an early response to drought. Further investigation of other eQTL hotspots suggests a role for a putative Homeobox leucine zipper protein 12 in relation to drought in potato.

    6. You have full text access to this OnlineOpen article
      The carbon bonus of organic nitrogen enhances nitrogen use efficiency of plants

      Oskar Franklin, Camila Aguetoni Cambui, Linda Gruffman, Sari Palmroth, Ram Oren and Torgny Näsholm

      Version of Record online: 29 JUN 2016 | DOI: 10.1111/pce.12772

  4. Technical Reports

    1. Gene mining in halophytes: functional identification of stress tolerance genes in Lepidium crassifolium

      Gábor Rigó, Ildikó Valkai, Dóra Faragó, Edina Kiss, Sara Van Houdt, Nancy Van de Steene, Matthew A. Hannah and László Szabados

      Version of Record online: 25 JUN 2016 | DOI: 10.1111/pce.12768

  5. Original Articles

    1. The influence of drought and heat stress on long-term carbon fluxes of bioenergy crops grown in the Midwestern USA

      Eva Joo, Mir Zaman Hussain, Marcelo Zeri, Michael D. Masters, Jesse N. Miller, Nuria Gomez-Casanovas, Evan H. DeLucia and Carl J. Bernacchi

      Version of Record online: 23 JUN 2016 | DOI: 10.1111/pce.12751

      Perennial grasses have been identified as being, in many ways, ideal feedstocks for bioenergy production. Yet side-by-side comparisons of key carbon fluxes and pools, particularly in response to climate extremes, for perennial grasses compared with the traditional row crops that they replace are needed. This research represents a long-term eddy covariance experiment, encompassing a severe drought, where three perennial ecosystems are compared with traditional row crops. The results show that perennial ecosystems are more resilient to drought than row crops in terms of both carbon uptake and storage and that a post-drought effect may be more likely in a perennial relative to annual ecosystem.

    2. Volatile compounds emitted by diverse phytopathogenic microorganisms promote plant growth and flowering through cytokinin action

      Ángela María Sánchez-López, Marouane Baslam, Nuria De Diego, Francisco José Muñoz, Abdellatif Bahaji, Goizeder Almagro, Adriana Ricarte-Bermejo, Pablo García-Gómez, Jun Li, Jan F. Humplík, Ondřej Novák, Lukáš Spíchal, Karel Doležal, Edurne Baroja-Fernández and Javier Pozueta-Romero

      Version of Record online: 16 JUN 2016 | DOI: 10.1111/pce.12759

      It is known that volatile emissions from some beneficial rhizosphere microorganisms promote plant growth. Here we show that volatile compounds (VCs) emitted by phylogenetically diverse rhizosphere and non-rhizhosphere bacteria and fungi (including plant pathogens and microbes that do not normally interact mutualistically with plants) promote growth and flowering of various plant species, including crops. Transcriptomic analyses revealed that many genes differentially expressed in Arabidopsis plants treated with VCs emitted by the fungal phytopathogen Alternaria alternata were also differentially expressed in plants exposed to VCs emitted by the plant growth promoting rhizobacterium Bacillus subtilis GB03, suggesting that plants react to microbial VCs through highly conserved regulatory mechanisms. The discovery that VCs from pathogenic microorganisms can have beneficial effects on plant growth and development extends knowledge of the diversity and complexity of the interactions involved in modulation of plant physiology, raising questions regarding the evolution of the processes, their ecological significance and potential applications.

  6. Reviews

    1. The β-cyanoalanine synthase pathway: beyond cyanide detoxification

      Marylou Machingura, Eitan Salomon, Joseph M. Jez and Stephen D. Ebbs

      Version of Record online: 16 JUN 2016 | DOI: 10.1111/pce.12755

      This review focuses on the cyanoalanine synthase pathway in plants and covers both its distribution within the plant kingdom and the broader range of functions now attributed to this pathway. For decades, this pathway has been described solely in terms of its role in cyanide detoxification. This has been an entrenched paradigm that has not changed measurably since the pathway's discovery in the 1960s. Research to date, however, has demonstrated that the functions of the pathway stretch beyond simple cyanide detoxification and encompass a range of functions that are fundamental to plant growth and development as well as to stress tolerance. There have been reviews in the past decade or so on cyanide, cyanogenesis and the range of biological degradation/assimilatory pathways for cyanide, but none have specifically focused on the distribution and function of the β-cyanoalanine synthase pathway as we do here. A fundamental goal of this manuscript is to expand the perception of this pathway and its functional importance.

  7. Opinions

    1. Toward an index of desiccation time to tree mortality under drought

      Chris J. Blackman, Sebastian Pfautsch, Brendan Choat, Sylvain Delzon, Sean M. Gleason and Remko A. Duursma

      Version of Record online: 16 JUN 2016 | DOI: 10.1111/pce.12758

  8. Original Articles

    1. Organelle redox autonomy during environmental stress

      Avishay Bratt, Shilo Rosenwasser, Andreas Meyer and Robert Fluhr

      Version of Record online: 16 JUN 2016 | DOI: 10.1111/pce.12746

      The subcellular glutathione disulfide/glutathione (GSH) redox state under various stress conditions was assessed using the redox reporter roGFP2 targeted to chloroplastic, mitochondrial, peroxisomal and cytosolic compartments. Under each stress, particular organelles were affected, indicating highly compartmentalized changes in the redox environment. In addition, early high levels of roGFP2 oxidation were found to be suggestive of latter cell death. The results show both cases of organelle autonomy as well as concerted organelle oxidative change.

    2. A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars

      Jiali Yan, Peitong Wang, Peng Wang, Meng Yang, Xingming Lian, Zhong Tang, Chao-Feng Huang, David E. Salt and Fang Jie Zhao

      Version of Record online: 16 JUN 2016 | DOI: 10.1111/pce.12747

      Excessive cadmium (Cd) accumulation in rice poses a risk to food safety. The tonoplast transporter OsHMA3 plays an important role in restricting Cd translocation from roots to shoots. Here, we identify a new loss-of-function allele of OsHMA3 in Japonica rice cultivars associated with high Cd accumulation in shoots and grain.

  9. Technical Reports

    1. A method for in situ monitoring of the isotope composition of tree xylem water using laser spectroscopy

      Till H. M. Volkmann, Kathrin Kühnhammer, Barbara Herbstritt, Arthur Gessler and Markus Weiler

      Version of Record online: 3 JUN 2016 | DOI: 10.1111/pce.12725

      Analysis of the stable isotope composition of xylem water is a powerful tool for assessing plant water relations, but available methodology has greatly limited the scope of isotope-based studies. Here, we introduce an in situ technique based on laser spectroscopy that allows monitoring of the isotope composition of xylem water in trees continuously and at high frequency while eliminating the need for costly and cumbersome destructive collection of plant material and laboratory-based processing. Results from field application demonstrate that temporal dynamics as well as spatial patterns of integration in xylem water isotope composition can now be resolved through direct measurement.

  10. Original Articles

    1. Powdery mildew suppresses herbivore-induced plant volatiles and interferes with parasitoid attraction in Brassica rapa

      Gaylord A. Desurmont, Hao Xu and Ted C. J. Turlings

      Version of Record online: 12 MAY 2016 | DOI: 10.1111/pce.12752

      The co-occurrence of different antagonists on a plant can have multitrophic consequences at the community level. Here we show that infection by a fungal pathogen, the powdery mildew Erysiphe cruciferarum, on Brassica rapa strongly reduces plant volatiles emitted in response to damage by the insect herbivore Pieris brassicae, and reduces plant attractiveness to the main natural enemy of P. brassicae, the parasitic wasp Cotesia glomerata. Herbivore performance was unaffected by the presence of powdery mildew, but parasitoid performance was negatively affected (decreased cocoon mass). From a pest management standpoint, the presence of powdery mildew on Brassica plants may affect the foraging efficiency of natural enemies, which may in turn have a negative impact on the regulation of pest populations.

    2. A dual system formed by the ARC and NR molybdoenzymes mediates nitrite-dependent NO production in Chlamydomonas

      Alejandro Chamizo-Ampudia, Emanuel Sanz-Luque, Ángel Llamas, Francisco Ocaña-Calahorro, Vicente Mariscal, Alfonso Carreras, Juan B. Barroso, Aurora Galván and Emilio Fernández

      Version of Record online: 12 MAY 2016 | DOI: 10.1111/pce.12739

      A mechanism for nitric oxide production from nitrite is described in plants. Nitrate Reductase is a key enzyme for this function, but not as a catalyser but as an electrons supplier for another molybdoenzyme (NOFNIR) to produce nitric oxide from nitrite in the presence of nitrate.

    3. Shoot tolerance mechanisms to iron toxicity in rice (Oryza sativa L.)

      Lin-Bo Wu, Yoshiaki Ueda, Shang-Kun Lai and Michael Frei

      Version of Record online: 12 MAY 2016 | DOI: 10.1111/pce.12733

    4. The Arabidopsis trichome is an active mechanosensory switch

      Li Hong Zhou, Shao Bao Liu, Peng Fei Wang, Tian Jian Lu, Feng Xu, Guy M. Genin and Barbara G. Pickard

      Version of Record online: 4 MAY 2016 | DOI: 10.1111/pce.12728

      The trichome is a sensor enabling Arabidopsis (and probably its agricultural relatives) to detect forces on the order of the weights that insects apply. When a trichome is bent or brushed, the unique tapering of its wall facilitates basal force transmission and focusing on a pliant zone, which consequently buckles. Force impinging on a surrounding skirt of cells is transduced to a chemical signal, evidenced as oscillation of cytosolic Ca2+. Elevation of apoplastic skirt cell pH is another indicator that the trichome has switched on chemical activity.

  11. Technical Reports

    1. Not a load of rubbish: simulated field trials in large-scale containers

      M. Hohmann, A. Stahl, J. Rudloff, B. Wittkop and R. J. Snowdon

      Version of Record online: 4 MAY 2016 | DOI: 10.1111/pce.12737

      Controlled-environment evaluations of complex traits rarely translate to field performance. To overcome this problem, we established a large-container plant growth system that allows detailed phenotyping of crop plants at field planting densities in deep soil. Yields of field-grown crops were able to be accurately predicted from container-grown plants, enabling detailed studies of stress response physiology under controlled conditions in relation to field performance.

  12. Special Issues

    1. The redox control of photorespiration: from biochemical and physiological aspects to biotechnological considerations

      Olivier Keech, Per Gardeström, Leszek A. Kleczkowski and Nicolas Rouhier

      Version of Record online: 21 APR 2016 | DOI: 10.1111/pce.12713

      This review discusses known and potential redox regulation mechanisms that might affect the photorespiratory enzymes as well as the peripheral enzymes associated to photorespiration. The reported post-translational modifications of key cysteine residues in these enzymes add another layer of complexity for their regulation, which has to be taken into account for any biotechnological strategies aiming at minimizing growth losses associated with photorespiration.

  13. Original Articles

    1. WD40-REPEAT 5a functions in drought stress tolerance by regulating nitric oxide accumulation in Arabidopsis

      Wen-Cheng Liu, Yun-Hui Li, Hong-Mei Yuan, Bing-Lei Zhang, Shuang Zhai and Ying-Tang Lu

      Version of Record online: 18 APR 2016 | DOI: 10.1111/pce.12723

    2. Polyamine oxidase 5 loss-of-function mutations in Arabidopsis thaliana trigger metabolic and transcriptional reprogramming and promote salt stress tolerance

      Xavier Zarza, Kostadin E. Atanasov, Francisco Marco, Vicent Arbona, Pedro Carrasco, Joachim Kopka, Vasileios Fotopoulos, Teun Munnik, Aurelio Gómez-Cadenas, Antonio F. Tiburcio and Rubén Alcázar

      Version of Record online: 13 APR 2016 | DOI: 10.1111/pce.12714

      Arabidopsis atpao5 loss-of-function mutants exhibit constitutive accumulation of thermospermine (tSpm) that associates with enhanced salt tolerance. tSpm triggers transcriptional and metabolic changes that involve promotion of ABA and JA pathways, accumulation of TCA cycle intermediates, compatible solutes along with other effects that additively contribute to salt tolerance. We provide evidence for the involvement of tSpm in plant abiotic stress tolerance.

  14. Reviews

  15. Special Issue

    1. NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity

      D. K. Gupta, L. B. Pena, M. C. Romero-Puertas, A. Hernández, M. Inouhe and L. M. Sandalio

      Version of Record online: 1 APR 2016 | DOI: 10.1111/pce.12711

      Under cadmium stress, respiratory burst oxydase homologs (RBOHs) differentially regulate H2O2 production, with RBOHC being the most important source of reactive oxygen species; anti-oxidative defences are also differentially regulated, with superoxide dismutase regulation by RBOHC, as well as the regulation of redox-couple GSH/GSSG ratio by RBOHC and D and the ASA/DHA by RBOHF being the most important factors. Our results also suggest that RBOHs can play an important role in regulating the root-to-shoot nutrient transport and are important players in the nutrient regulatory hub and, for instance, can be of potential interest in biotechnology to protect shoot from heavy metals.

    2. Nitric oxide function in plant abiotic stress

      Nurun Nahar Fancy, Ann-Kathrin Bahlmann and Gary J. Loake

      Version of Record online: 1 MAR 2016 | DOI: 10.1111/pce.12707

      In this manuscript, we focus on the current state-of-the-art regarding the role of nitric oxide (NO) and S-nitrosylation during abiotic stress responses in plants. This is significant because NO and S-nitrosylation are emerging as important players in plant abiotic stress signalling. The paper should be of interest to readers in the areas of plant abiotic stress, signalling, redox regulation, NO function and S-nitrosylation.

  16. Original Articles

    1. Grapevine petioles are more sensitive to drought induced embolism than stems: evidence from in vivo MRI and microcomputed tomography observations of hydraulic vulnerability segmentation

      Uri Hochberg, Caetano Albuquerque, Shimon Rachmilevitch, Herve Cochard, Rakefet David-Schwartz, Craig R. Brodersen, Andrew McElrone and Carel W. Windt

      Version of Record online: 12 FEB 2016 | DOI: 10.1111/pce.12688

      In this work we examined the “hydraulic vulnerability segmentation” hypothesis which predicts that expendable distal organs are more susceptible to waterߚstress induced cavitation than the main stem of the plant. We explored the differences in embolism formation of intact petioles and stems of grapevines by means of MRI and microCT. The paper presents the first direct evidence and the first imaging of hydraulic vulnerability segmentation in living plants and highlight its importance in grapevine responses to severe water stress.

  17. Commentaries

    1. Broadening the spectrum of photosynthesis in the grass, Alloteropsis semialata

      Gregory Reeves and Julian M. Hibberd

      Version of Record online: 5 FEB 2016 | DOI: 10.1111/pce.12685

  18. Original Articles

    1. Evolutionary implications of C3–C4 intermediates in the grass Alloteropsis semialata

      Marjorie R. Lundgren, Pascal-Antoine Christin, Emmanuel Gonzalez Escobar, Brad S. Ripley, Guillaume Besnard, Christine M. Long, Paul W. Hattersley, Roger P. Ellis, Richard C. Leegood and Colin P. Osborne

      Version of Record online: 21 JAN 2016 | DOI: 10.1111/pce.12665

      C4 photosynthesis is a complex trait that increases productivity in some conditions, but the changes linked to its evolutionary assembly remain poorly understood. In this study, we show for the first time that the grass Alloteropsis semialata, the only species known with both C3 and C4 individuals, also includes C3–C4 intermediates. The presence in a single species of individuals spanning the C3, C3–C4 and C4 continuum opens new avenues for ecological, physiological and genetic comparisons to understand the changes that led to the emergence of this novel pathway.

  19. Special Issue

    1. Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco

      Claire Rosnoblet, Hervé Bègue, Cécile Blanchard, Carole Pichereaux, Angélique Besson-Bard, Sébastien Aimé and David Wendehenne

      Version of Record online: 18 JAN 2016 | DOI: 10.1111/pce.12686

      In a previous study, we demonstrated that the chaperone-like protein NtCdc48 is regulated by S-nitrosylation in tobacco cells undergoing an immune response triggered by cryptogein, an elicitin produced by the oomycete Phytophthora cryptogea. In the present original study, we further investigated the function of this chaperone in cryptogein signalling and cryptogein-triggered hypersensitive-like cell death. We reported that only a small proportion of the overall NtCdc48 protein population undergoes S-nitrosylation. However, using different strategies such as gel filtration in native conditions, immunoprecipitation and the generation of a tobacco cell line overexpressing NtCdc48, we demonstrated that this protein is mobilized in response to cryptogein, is present as a hexameric and active complex and interacts with numerous partners related to proteasome-dependent degradation, subcellular trafficking and redox regulation. Importantly, our study highlighted a role for NtCdc48 in cryptogein-triggered cell death. Altogether, this investigation designs NtCdc48 as a new component of plant immunity.

  20. Reviews

    1. The nitrogen–potassium intersection: membranes, metabolism, and mechanism

      Devrim Coskun, Dev T. Britto and Herbert J. Kronzucker

      Version of Record online: 18 JAN 2016 | DOI: 10.1111/pce.12671

      This review summarizes fundamental intersections between the pathways of inorganic nitrogen (NH4+ and NO3) and potassium (K+) acquisition in plants. Uptake, storage, translocation and metabolism are discussed at levels of organization ranging from molecular-genetic processes to whole-plant physiology. The regulation and optimization of plant growth, yield, metabolism and water-use efficiency are discussed in this nutritional context.


  1. 1 - 37