Plant, Cell & Environment

Cover image for Vol. 39 Issue 11

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Keith Mott

Impact Factor: 6.169

ISI Journal Citation Reports © Ranking: 2015: 10/209 (Plant Sciences)

Online ISSN: 1365-3040


  1. 1 - 37
  1. Original Articles

    1. Non-selective cation channel activity of aquaporin AtPIP2;1 regulated by Ca2+ and pH

      Caitlin S. Byrt, Manchun Zhao, Mohamad Kourghi, Jayakumar Bose, Sam W. Henderson, Jiaen Qiu, Matthew Gilliham, Carolyn Schultz, Manuel Schwarz, Sunita A. Ramesh, Andrea Yool and Steve Tyerman

      Version of Record online: 18 OCT 2016 | DOI: 10.1111/pce.12832

      The paradigm that aquaporins may only allow permeation of neutral solutes has been challenged by examples of some animal aquaporins that can act as ion channels. Here, we reveal that AtPIP2;1 is permeable to both water and Na+ in heterologous systems. The AtPIP2;1 ionic conductance is inhibited by low pH and Ca2+ similar to previous observations for non-selective cation channels indicating that AtPIP2;1 is a candidate for facilitating Na+ flux across the plasma membrane of root cells and other cells that express PIP2;1, such as guard cells.

    2. Nitrogen fertilization and δ18O of CO2 have no effect on 18O-enrichment of leaf water and cellulose in Cleistogenes squarrosa (C4) – is VPD the sole control?

      Hai Tao Liu, Xiao Ying Gong, Rudi Schäufele, Fang Yang, Regina Theresia Hirl, Anja Schmidt and Hans Schnyder

      Version of Record online: 17 OCT 2016 | DOI: 10.1111/pce.12824

      The 18O enrichment of cellulose stores physiological information from 18O enrichment of leaf water, thus is of great interest to a range of scientific disciplines including palaeoecology, global change science and plant physiology. However, the propagation of the 18O signal from leaf water to cellulose is associated with some degree of signal attenuation (termed pexpx) that is not well understood. Here, we show that the attenuation responds to VPD in a C4 grass (Cleistogenes squarrosa), but was not influenced by nitrogen nutrition or the oxygen isotope composition of CO2.

  2. Reviews

    1. Optimal plant water economy

      Thomas N Buckley, Lawren Sack and Graham D Farquhar

      Version of Record online: 17 OCT 2016 | DOI: 10.1111/pce.12823

      We review the theory of optimal regulation of plant water loss and suggest directions for continuing research, including (i) improving analytical approximations to the classical theory, as current approximations ignore boundary layer and mesophyll resistance and represent the theory quite poorly; (ii) exploring the role of cyclical variations in hydraulic conductance in optimal diurnal regulation; (iii) distinguishing optimal stomatal responses from changes in stomatal conductance that emerge from optimal shifts in carbon partitioning at long time scales; and (iv) further developing the whole-plant theory and testing its predictions.

  3. Original Articles

    1. Association between water and carbon dioxide transport in leaf plasma membranes: assessing the role of aquaporins

      Manchun Zhao, Hwei-Ting Tan, Johannes Scharwies, Kara Levin, John R Evans and Stephen D Tyerman

      Version of Record online: 14 OCT 2016 | DOI: 10.1111/pce.12830

      The osmotic permeability to water and diffusive permeability to CO2 of plasma membranes isolated from leaves were examined to assess co-regulation of transport through aquaporins. The diffusive permeability to CO2 was not limited by unstirred layers or limitations from carbonic anhydrase used in the method. While showing very different osmotic water permeability, plasma membranes from pea and Arabidopsis showed similar CO2 permeability. The temperature dependence of water and CO2 permeation was measured indicating involvement of aquaporins; however, the inhibition of aquaporins reduced water permeability but did not change CO2 permeability. The two permeabilities were positively correlated but only weakly so. These observations are relevant to understanding the role of aquaporins in mesophyll conductance to CO2, and the proposed co-regulation of water and CO2 transport in leaves.

    2. Freezing behaviours in wintering Cornus florida flower bud tissues revisited using MRI

      Masaya Ishikawa, Hiroyuki Ide, Hideyuki Yamazaki, Hiroki Murakawa, Kazuyuki Kuchitsu, William S. Price and Yoji Arata

      Version of Record online: 13 OCT 2016 | DOI: 10.1111/pce.12813

      How plant tissues in a complex organ respond to freezing is not readily visible, and the diversity of such responses (freezing behaviours) and their controlling mechanisms remain unexplored in spite of their importance in cold hardiness mechanisms. In this study, the use of high-resolution magnetic resonance imaging (MRI) allowed visualization of freezing behaviours in Cornus florida flower bud tissues; only anthers and ovules remain stably supercooled to −14 to −21 °C while other floral tissues tolerate extracellular freezing. The abrupt breakdown of supercooled individual ovules and anthers resulted in their all-or-nothing type of injuries. Only gametophytic tissues remaining unfrozen are a novel freezing behaviour in flower buds, and the distribution of ice nucleation activity in flower buds corroborated such a freezing behaviour.

    3. Combined use of herbivore-induced plant volatiles and sex pheromones for mate location in braconid parasitoids

      Hao Xu, Gaylord Desurmont, Thomas Degen, Guoxin Zhou, Diane Laplanche, Luka Henryk and Ted C.J. Turlings

      Version of Record online: 7 OCT 2016 | DOI: 10.1111/pce.12818

      We demonstrate that parasitic wasps use herbivore-induced plant volatiles (HIPVs) in combination with sex pheromones to locate mates, and foraging for hosts and mates are both orientated by HIPVs in parasitoids. To our knowledge, no other study has specifically addressed the use of HIPVs in mate location of parasitoids, and the inclusion of four species of wasps allows us not only to draw several general conclusions but also to call attention to the differences among species in terms of mate-searching strategies.

    4. Genome interrogation for novel salinity tolerant Arabidopsis mutants

      Niels van Tol, Johan Pinas, Henk Schat, Paul J. J. Hooykaas and Bert J. van der Zaal

      Version of Record online: 7 OCT 2016 | DOI: 10.1111/pce.12805

      We have investigated whether a capacity to withstand normally lethal levels of salinity can be evoked in the model plant species Arabidopsis thaliana by inducing large scale and more or less random distortion of gene expression patterns. To this end, we have used zinc finger artificial transcription factors (ZF-ATFs). Within a relatively small collection of Arabidopsis lines expressing ZF-ATFs, we found a large number of lines that were strongly tolerant to 100 mM NaCl. We demonstrate that several ZF-ATF gene constructs act as dominant and heritable agents for salinity tolerance.

    5. You have full text access to this OnlineOpen article
      Time of day determines Arabidopsis transcriptome and growth dynamics under mild drought

      Marieke Dubois, Hannes Claeys, Lisa Van den Broeck and Dirk Inzé

      Version of Record online: 7 OCT 2016 | DOI: 10.1111/pce.12809

      Drought stress forms a major environmental constraint for agriculture worldwide. Drought triggers specific responses in plants, depending on stress severity and duration, and on the organ and its developmental stage. In young, developing leaves, growth is repressed as a mechanism to save water and energy, but the underlying molecular basis is largely unknown. Here, we present a novel approach to explore the short-term molecular mechanisms controlling leaf growth inhibition following drought and show that the time of day is a major determinant of drought responses, affecting both growth and transcriptome dynamics.

    6. C4 photosynthesis in C3 rice: a theoretical analysis of biochemical and anatomical factors

      Shuyue Wang, Danny Tholen and Xin-Guang Zhu

      Version of Record online: 7 OCT 2016 | DOI: 10.1111/pce.12834

      A 3D two-cell reaction diffusion model was developed to explore the photosynthetic efficiency of the C4 metabolic cycle in the anatomical and biochemical background of a C3 leaf. Our results show that integrating a C4 metabolic pathway into rice leaves may lead to an improved photosynthesis under current ambient CO2 concentrations. Partitioning of energy between C3 and C4 photosynthesis and the partitioning of Rubisco between mesophyll and bundle-sheath cells are decisive factors controlling photosynthetic efficiency in an engineered C3–C4 leaf.

    7. You have full text access to this OnlineOpen article
      A user-friendly means to scale from the biochemistry of photosynthesis to whole crop canopies and production in time and space – development of Java WIMOVAC

      Qingfeng Song, Dairui Chen, Stephen P. Long and Xin-Guang Zhu

      Version of Record online: 7 OCT 2016 | DOI: 10.1111/pce.12816

      Windows Intuitive Model of Vegetation response to Atmosphere and Climate Change (WIMOVAC) has been used widely as a generic mechanistically rich model scaling from biochemistry of leaf photosynthesis to canopy processes and plant production. This paper describes an open-source JAVA user-friendly version of WIMOVAC. It is platform independent and can be easily downloaded to a laptop and used without any prior programming skill.

    8. You have full text access to this OnlineOpen article
      Phenotypic plasticity to altered apical bud temperature in Cucumis sativus: more leaves-smaller leaves and vice versa

      Andreas Savvides, Wim van Ieperen, Janneke A. Dieleman and Leo F.M. Marcelis

      Version of Record online: 7 OCT 2016 | DOI: 10.1111/pce.12835

      In a 28 days study, we maintained temperature differences between apical bud (Tbud) and the rest of the plant (Tplant) ranging from −7 to +8 °C using a custom-made bud temperature control system. Differences between Tbud and Tplant considerably influenced vertical leaf area distribution over the shoot: increasing Tbud beyond Tplant resulted in more and smaller leaves, while decreasing Tbud below Tplant resulted in less and larger leaves. The trade-off between leaf number and leaf area resulted in phenotypic alterations that cannot be predicted, for example, by crop models, when assuming plant temperature uniformity.

    9. Hydrogen isotopic differences between C3 and C4 land plant lipids: consequences of compartmentation in C4 photosynthetic chemistry and C3 photorespiration

      Youping Zhou, Kliti Grice, Hilary Stuart-Williams, Charles H. Hocart, Arthur Gessler and Graham D. Farquhar

      Version of Record online: 3 OCT 2016 | DOI: 10.1111/pce.12821

      • Analysed the 2H/1H ratio of carbon-bound hydrogens of controlled and field grown C3 and C4 plant leaf lipids.
      • C3 versus C4 differences in lipid-over-leaf-water 2H depletion are lipid pathway-dependent.
      • Compartmentation and photorespiration identified as the biochemical bases for such differences.
    10. Segregation of nitrogen use between ammonium and nitrate of ectomycorrhizas and beech trees

      Martin Leberecht, Michael Dannenmann, Javier Tejedor, Judy Simon, Heinz Rennenberg and Andrea Polle

      Version of Record online: 3 OCT 2016 | DOI: 10.1111/pce.12820

      Ammonium (NH4+) and nitrate (NO3) uptake of beech (Fagus sylvatica) and their associated ectomycorrhizal (EM) assemblages were studied by 15N labelling under field conditions.

      In native EM assemblages, long-term and short-term 15N uptake from NH4+ was higher than that from NO3, regardless of season, water availability and site exposure, whereas in beech long-term 15N uptake from NO3 was higher than that from NH4+.

      The transfer rates from the EM to beech were lower for 15N from NH4+ than that from NO3.

      15N content in EM was correlated with 15NH4+, but not with 15NO3-derived N uptake of the plant, suggesting a stronger control of the EM assemblage on N provision to the host from NH4+ than that from NO3.

  4. Reviews

    1. Xylem and stomata, coordinated through time and space

      Timothy J Brodribb, Scott AM McAdam and Madeline R Carins Murphy

      Version of Record online: 30 SEP 2016 | DOI: 10.1111/pce.12817

      Vascular plants incur penalties for allowing leaf hydration to range outside strict limits of hydration. Xylem and stomatal tissues regulate the acquisition and loss of water in leaves, and they must work together to maintain a safe level of leaf hydration. This review examines how xylem and stomatal tissues are coordinated to achieve this important role.

  5. Original Articles

    1. Differential antioxidant response between two Symbiodinium species from contrasting environments

      S. Roberty, P. Furla and J. -C. Plumier

      Version of Record online: 30 SEP 2016 | DOI: 10.1111/pce.12825

      Climate changes are responsible for the disruption of the symbiosis between cnidarians and their photosynthetic Symbiodinium. Although this phenomenon, known as coral bleaching, is a consequence to oxidative stress, current understanding of the functioning of the antioxidant network is insufficient for Symbiodinium. We present a comparative analysis on two Symbiodinium species from tropical and temperate environments that highlights differences in the responses of the antioxidant network with the temperate species being more tolerant to an endogenous oxidative stress.

  6. Technical Reports

    1. Simple and robust methods for remote sensing of canopy chlorophyll content: a comparative analysis of hyperspectral data for different types of vegetation

      Yoshio Inoue, Martine Guérif, Frédéric Baret, Andrew Skidmore, Anatoly Gitelson, Martin Schlerf, Roshanak Darvishzadeh and Albert Olioso

      Version of Record online: 21 SEP 2016 | DOI: 10.1111/pce.12815

      Because the canopy chlorophyll content (CCC) is an essential ecophysiological variable for photosynthetic functioning, remote sensing of CCC is vital for a wide range of ecological and agricultural applications. Simple and robust algorithms were explored for spectral assessment of CCC using the diverse hyperspectral datasets for six vegetation types (rice, wheat, corn, soybean, sugar beet, natural grass) acquired in four locations (Japan, France, Italy, USA). A simple model using the ratio spectral index RSI(R815,R704) with the reflectance at 815 and 704 nm, proved to have the highest accuracy and applicability based on comprehensive analysis on the accuracy, linearity, sensitivity and applicability of various spectral models. The model would work as a robust algorithm for canopy-chlorophyll-metre and/or remote sensing of CCC in ecosystem and regional scales.

  7. Reviews

    1. Osmotic adjustment is a prime drought stress adaptive engine in support of plant production

      Abraham Blum

      Version of Record online: 20 SEP 2016 | DOI: 10.1111/pce.12800

      Osmotic adjustment has long been recognized as an important mechanism for adaptation to drought stress in native and in crop plants. During the late 1980s and early 2000s, contrarian views were published arguing that OA was not important for adaptation or that it was important only for survival but not for plant productivity. These views still resonate occasionally in the literature; therefore, this paper offers a critical review of evidence proving that OA support crop production under drought stress.

    2. Biochemical basis of sulphenomics: how protein sulphenic acids may be stabilized by the protein microenvironment

      P. Trost, S. Fermani, M. Calvaresi and M. Zaffagnini

      Version of Record online: 7 SEP 2016 | DOI: 10.1111/pce.12791

      This review highlights the importance of acidity and nucleophilicity of protein cysteine thiols in determining the rate of H2O2-mediated primary oxidation to sulphenic acids. The stability and reactivity of sulphenic acids is also investigated, being strictly correlated to the cysteine microenvironment and dependent upon structural determinants, which are specific of each protein sensitive to oxidation. These findings reinforce the prominent role of cysteine sulphenic acids in redox signalling, but a combination of biochemical, structural and computational approaches is mandatory to get insight into the kinetic and thermodynamics factors controlling cysteine oxidation.

  8. Original Articles

    1. Variation of DNA methylation patterns associated with gene expression in rice (Oryza sativa) exposed to cadmium

      Sheng Jun Feng, Xue Song Liu, Hua Tao, Shang Kun Tan, Shan Shan Chu, Youko Oono, Xian Duo Zhang, Jian Chen and Zhi Min Yang

      Version of Record online: 7 SEP 2016 | DOI: 10.1111/pce.12793

      This study identified global differential DNA methylation marks associated with gene expression and functional consequences in rice exposed to Cd. Widespread differences were identified in CG and non-CG methylation marks between Cd-exposed and Cd-free rice genomes. A group of genes encoding metal transporters, Cd-detoxified proteins and metal-related transcription factors that were differentially methylated were found to regulate rice tolerance to Cd stress. Our data provide an insight into DNA methylation pattern associated with activation of specific genes responsible for Cd uptake, accumulation and detoxification.

  9. Reviews

    1. An ecophysiological and developmental perspective on variation in vessel diameter

      Uwe G. Hacke, Rachel Spicer, Stefan G. Schreiber and Lenka Plavcová

      Version of Record online: 9 AUG 2016 | DOI: 10.1111/pce.12777

      Variation in xylem vessel diameter impacts transport efficiency, vulnerability to freezing-induced embolism, and other aspects of plant biology. This review provides a synthesis of the ecophysiological implications of variation in lumen diameter together with a summary of our current understanding of vessel development and its endogenous regulation. Emphasis is placed on the presumed role of auxin at multiple developmental stages.

  10. Original Articles

    1. You have full text access to this OnlineOpen article
      Differential expression of microRNAs and potential targets under drought stress in barley

      Jannatul Ferdous, Juan Carlos Sanchez-Ferrero, Peter Langridge, Linda Milne, Jamil Chowdhury, Chris Brien and Penny J. Tricker

      Version of Record online: 4 AUG 2016 | DOI: 10.1111/pce.12764

      A role for microRNAs in regulating gene responses to abiotic stress has been well established but translating this information to practical crop improvement has proved difficult, because we have had little knowledge about genotypic stress-induced variation in expression of miRNAs and their targets. MicroRNA/gene target interaction is usually predicted from bioinformatic analysis of small RNA and degradome sequencing data. Of 11 predicted, drought-regulated microRNAs we were able to show inverse correlation of only two microRNA/ target pairs under drought, and this was genotype dependent. Our results show genotypic variation in miRNA expression and provide evidence of drought-responsive regulation of important microRNA target genes.

    2. Plumbing the depths: extracellular water storage in specialized leaf structures and its functional expression in a three-domain pressure –volume relationship

      Hoa T. Nguyen, Patrick Meir, Joe Wolfe, Maurizio Mencuccini and Marilyn C. Ball

      Version of Record online: 25 JUL 2016 | DOI: 10.1111/pce.12788

      The pressure-volume relationships in field grown leaves of the mangrove, Avicennia marina, exhibited three domains dominated successively by 1) the presence and consumption of extracellular water, 2) variable turgor and loss of intracellular water, and 3) osmotic behavior of flaccid cells and plasmolysis. Multiple sites of extracellular water storage were identified, including hollow trichomes and novel structures named “cisternae”. When fully charged, extracellular and cellular water storage could support a typical evaporation rate of 1 mmol m−2s−1 for 54 and 50 min, respectively, before turgor loss was reached. This study emphasizes the importance of leaf anatomy for the interpretation of PV curves, and identifies extracellular water storage sites that enable transient water use without substantive turgor loss when other factors, such as high soil salinity, constrain rates of water transport.

    3. You have full text access to this OnlineOpen article
      Nitric oxide-fixation by non-symbiotic haemoglobin proteins in Arabidopsis thaliana under N-limited conditions

      Gitto Thomas Kuruthukulangarakoola, Jiangli Zhang, Andreas Albert, Barbro Winkler, Hans Lang, Franz Buegger, Frank Gaupels, Werner Heller, Bernhard Michalke, Hakan Sarioglu, Jörg-Peter Schnitzler, Kim Henrik Hebelstrup, Jörg Durner and Christian Lindermayr

      Version of Record online: 25 JUL 2016 | DOI: 10.1111/pce.12773

      This study reports about a NO-fixing mechanism in Arabidopsis, which allows the fixation of atmospheric NO into nitrogen metabolism. Non-symbiotic haemoglobin class 1 and class 2 were identified as key proteins of the NO-fixation pathway converting NO to nitrate, which is further introduced into the N-metabolism. This mechanism is probably important under conditions with limited N supply via the soil. Moreover, the plant-based NO uptake lowers the concentration of insanitary atmospheric NOx, and in this context, NO-fixation can be beneficial to air quality.

    4. Diacylglycerol kinases activate tobacco NADPH oxidase-dependent oxidative burst in response to cryptogein

      Jean-Luc Cacas, Patricia Gerbeau-Pissot, Jérôme Fromentin, Catherine Cantrel, Dominique Thomas, Emmanuelle Jeannette, Tetiana Kalachova, Sébastien Mongrand, Françoise Simon-Plas and Eric Ruelland

      Version of Record online: 20 JUL 2016 | DOI: 10.1111/pce.12771

      Cryptogein is a protein secreted by the oomycete Phytophthora cryptogea that activates defence mechanisms in tobacco. We show here in BY-2 tobacco suspension cells that phosphatidic acid rapidly accumulates in response to cryptogein because of the coordinated onset of phosphoinositide-dependent phospholipase C and diacylglycerol kinase activities. Both enzyme specific inhibitors and silencing of the phylogenetic cluster III of the tobacco DGK family were found to reduce PA production upon elicitation and to strongly decrease the RBOHD-mediated oxidative burst. This establishes that phosphatidic acid production by diacylglycerol kinases is upstream of the oxidative burst in response to cryptogein.

    5. You have full text access to this OnlineOpen article
      The carbon bonus of organic nitrogen enhances nitrogen use efficiency of plants

      Oskar Franklin, Camila Aguetoni Cambui, Linda Gruffman, Sari Palmroth, Ram Oren and Torgny Näsholm

      Version of Record online: 29 JUN 2016 | DOI: 10.1111/pce.12772

    6. Volatile compounds emitted by diverse phytopathogenic microorganisms promote plant growth and flowering through cytokinin action

      Ángela María Sánchez-López, Marouane Baslam, Nuria De Diego, Francisco José Muñoz, Abdellatif Bahaji, Goizeder Almagro, Adriana Ricarte-Bermejo, Pablo García-Gómez, Jun Li, Jan F. Humplík, Ondřej Novák, Lukáš Spíchal, Karel Doležal, Edurne Baroja-Fernández and Javier Pozueta-Romero

      Version of Record online: 16 JUN 2016 | DOI: 10.1111/pce.12759

      It is known that volatile emissions from some beneficial rhizosphere microorganisms promote plant growth. Here we show that volatile compounds (VCs) emitted by phylogenetically diverse rhizosphere and non-rhizhosphere bacteria and fungi (including plant pathogens and microbes that do not normally interact mutualistically with plants) promote growth and flowering of various plant species, including crops. Transcriptomic analyses revealed that many genes differentially expressed in Arabidopsis plants treated with VCs emitted by the fungal phytopathogen Alternaria alternata were also differentially expressed in plants exposed to VCs emitted by the plant growth promoting rhizobacterium Bacillus subtilis GB03, suggesting that plants react to microbial VCs through highly conserved regulatory mechanisms. The discovery that VCs from pathogenic microorganisms can have beneficial effects on plant growth and development extends knowledge of the diversity and complexity of the interactions involved in modulation of plant physiology, raising questions regarding the evolution of the processes, their ecological significance and potential applications.

    7. Shoot tolerance mechanisms to iron toxicity in rice (Oryza sativa L.)

      Lin-Bo Wu, Yoshiaki Ueda, Shang-Kun Lai and Michael Frei

      Version of Record online: 12 MAY 2016 | DOI: 10.1111/pce.12733

    8. The Arabidopsis trichome is an active mechanosensory switch

      Li Hong Zhou, Shao Bao Liu, Peng Fei Wang, Tian Jian Lu, Feng Xu, Guy M. Genin and Barbara G. Pickard

      Version of Record online: 4 MAY 2016 | DOI: 10.1111/pce.12728

      The trichome is a sensor enabling Arabidopsis (and probably its agricultural relatives) to detect forces on the order of the weights that insects apply. When a trichome is bent or brushed, the unique tapering of its wall facilitates basal force transmission and focusing on a pliant zone, which consequently buckles. Force impinging on a surrounding skirt of cells is transduced to a chemical signal, evidenced as oscillation of cytosolic Ca2+. Elevation of apoplastic skirt cell pH is another indicator that the trichome has switched on chemical activity.

  11. Special Issues

    1. The redox control of photorespiration: from biochemical and physiological aspects to biotechnological considerations

      Olivier Keech, Per Gardeström, Leszek A. Kleczkowski and Nicolas Rouhier

      Version of Record online: 21 APR 2016 | DOI: 10.1111/pce.12713

      This review discusses known and potential redox regulation mechanisms that might affect the photorespiratory enzymes as well as the peripheral enzymes associated to photorespiration. The reported post-translational modifications of key cysteine residues in these enzymes add another layer of complexity for their regulation, which has to be taken into account for any biotechnological strategies aiming at minimizing growth losses associated with photorespiration.

  12. Original Articles

    1. WD40-REPEAT 5a functions in drought stress tolerance by regulating nitric oxide accumulation in Arabidopsis

      Wen-Cheng Liu, Yun-Hui Li, Hong-Mei Yuan, Bing-Lei Zhang, Shuang Zhai and Ying-Tang Lu

      Version of Record online: 18 APR 2016 | DOI: 10.1111/pce.12723

    2. Polyamine oxidase 5 loss-of-function mutations in Arabidopsis thaliana trigger metabolic and transcriptional reprogramming and promote salt stress tolerance

      Xavier Zarza, Kostadin E. Atanasov, Francisco Marco, Vicent Arbona, Pedro Carrasco, Joachim Kopka, Vasileios Fotopoulos, Teun Munnik, Aurelio Gómez-Cadenas, Antonio F. Tiburcio and Rubén Alcázar

      Version of Record online: 13 APR 2016 | DOI: 10.1111/pce.12714

      Arabidopsis atpao5 loss-of-function mutants exhibit constitutive accumulation of thermospermine (tSpm) that associates with enhanced salt tolerance. tSpm triggers transcriptional and metabolic changes that involve promotion of ABA and JA pathways, accumulation of TCA cycle intermediates, compatible solutes along with other effects that additively contribute to salt tolerance. We provide evidence for the involvement of tSpm in plant abiotic stress tolerance.

  13. Reviews

  14. Special Issues

    1. NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity

      D. K. Gupta, L. B. Pena, M. C. Romero-Puertas, A. Hernández, M. Inouhe and L. M. Sandalio

      Version of Record online: 1 APR 2016 | DOI: 10.1111/pce.12711

      Under cadmium stress, respiratory burst oxydase homologs (RBOHs) differentially regulate H2O2 production, with RBOHC being the most important source of reactive oxygen species; anti-oxidative defences are also differentially regulated, with superoxide dismutase regulation by RBOHC, as well as the regulation of redox-couple GSH/GSSG ratio by RBOHC and D and the ASA/DHA by RBOHF being the most important factors. Our results also suggest that RBOHs can play an important role in regulating the root-to-shoot nutrient transport and are important players in the nutrient regulatory hub and, for instance, can be of potential interest in biotechnology to protect shoot from heavy metals.

  15. Special Issue

    1. Nitric oxide function in plant abiotic stress

      Nurun Nahar Fancy, Ann-Kathrin Bahlmann and Gary J. Loake

      Version of Record online: 1 MAR 2016 | DOI: 10.1111/pce.12707

      In this manuscript, we focus on the current state-of-the-art regarding the role of nitric oxide (NO) and S-nitrosylation during abiotic stress responses in plants. This is significant because NO and S-nitrosylation are emerging as important players in plant abiotic stress signalling. The paper should be of interest to readers in the areas of plant abiotic stress, signalling, redox regulation, NO function and S-nitrosylation.

    2. Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco

      Claire Rosnoblet, Hervé Bègue, Cécile Blanchard, Carole Pichereaux, Angélique Besson-Bard, Sébastien Aimé and David Wendehenne

      Version of Record online: 18 JAN 2016 | DOI: 10.1111/pce.12686

      In a previous study, we demonstrated that the chaperone-like protein NtCdc48 is regulated by S-nitrosylation in tobacco cells undergoing an immune response triggered by cryptogein, an elicitin produced by the oomycete Phytophthora cryptogea. In the present original study, we further investigated the function of this chaperone in cryptogein signalling and cryptogein-triggered hypersensitive-like cell death. We reported that only a small proportion of the overall NtCdc48 protein population undergoes S-nitrosylation. However, using different strategies such as gel filtration in native conditions, immunoprecipitation and the generation of a tobacco cell line overexpressing NtCdc48, we demonstrated that this protein is mobilized in response to cryptogein, is present as a hexameric and active complex and interacts with numerous partners related to proteasome-dependent degradation, subcellular trafficking and redox regulation. Importantly, our study highlighted a role for NtCdc48 in cryptogein-triggered cell death. Altogether, this investigation designs NtCdc48 as a new component of plant immunity.

  16. Reviews

    1. The nitrogen–potassium intersection: membranes, metabolism, and mechanism

      Devrim Coskun, Dev T. Britto and Herbert J. Kronzucker

      Version of Record online: 18 JAN 2016 | DOI: 10.1111/pce.12671

      This review summarizes fundamental intersections between the pathways of inorganic nitrogen (NH4+ and NO3) and potassium (K+) acquisition in plants. Uptake, storage, translocation and metabolism are discussed at levels of organization ranging from molecular-genetic processes to whole-plant physiology. The regulation and optimization of plant growth, yield, metabolism and water-use efficiency are discussed in this nutritional context.


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