Common ragweed (Ambrosia artemisiifolia L.): allergenicity and molecular characterization of pollen after plant exposure to elevated NO2
Feng Zhao, Amr Elkelish, Jörg Durner, Christian Lindermayr, J. Barbro Winkler, Franziska Ruёff, Heidrun Behrendt, Claudia Traidl-Hoffmann, Andreas Holzinger, Werner Kofler, Paula Braun, Christine von Toerne, Stefanie M. Hauck, Dieter Ernst and Ulrike Frank
Article first published online: 19 SEP 2015 | DOI: 10.1111/pce.12601
Our study shows the influence of abiotic factors on the highly allergenic pollen from ragweed. Climate change and global warming as well as other environmental conditions have an influence on the plant and therefore might have also an influence on the allergenic potential of their pollen. We did proteomic studies and infrared spectroscopic analyses of pollen from the non-model plant Ambrosia artemisiifolia to give more insight on how the pollen reacts to elevated NO2. Allergenic proteins as well as allergen transcripts were increased under elevated NO2, and differences in cell wall components have been detected. Additionally, S-nitrosylation of pollen proteins was shown and higher allergen recognition by immuno-blot have been detected. Allergies are increasing, and the allergenic potential of the pollen is influenced by environmental factors. We used highly sophisticated methods, such as two-dimensional difference gel electrophoresis analyses, and were able to detect, beside the well-known Ambrosia allergens, a homolog to another plant allergen that reacted also with the sera of ragweed allergenic patients and therefore might modulate the Ambrosia allergenic response.