Development, Growth & Differentiation

Cover image for Vol. 58 Issue 1

Edited By: Harukazu Nakamura

Impact Factor: 2.42

ISI Journal Citation Reports © Ranking: 2014: 26/41 (Developmental Biology); 124/184 (Cell Biology)

Online ISSN: 1440-169X

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  1. Multi-color FISH facilitates analysis of cell-type diversification and developmental gene regulation in the Parasteatoda spider embryo

    Yasuko Akiyama-Oda and Hiroki Oda

    Article first published online: 9 FEB 2016 | DOI: 10.1111/dgd.12263

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    We established a method for multi-color fluorescence in situ hybridization (mFISH) in the spider embryo. This mFISH technique allowed quadruple staining using four kinds of labels for RNA probes, and it was used for the analysis of overlapping gene expression in the cell level, the relative quantification of gene expression, and combined examination with embryonic RNA interference.

  2. Heterogeneity of chromatoid bodies in adult pluripotent stem cells of planarian Dugesia japonica

    Makoto Kashima, Nobuyoshi Kumagai, Kiyokazu Agata and Norito Shibata

    Article first published online: 9 FEB 2016 | DOI: 10.1111/dgd.12268

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    We show molecular heterogeneity of chromatoid bodies (CBs), which are unique cytoplasmic ribonucleoprotein (RNP) granules of the neoblasts in planarians. We revealed that DjCBC-1-positive CBs, termed cCBs, were distinct from Y-12/DjPiwiC-immunoreactive CBs.

  3. Blastocysts derivation from somatic cell fusion with premature oocytes (prematuration somatic cell fusion)

    Islam M. Saadeldin, Candrani Khoirinaya, Su Jin Kim, Joon Ho Moon, Essam Almadaly and Byeong Chun Lee

    Article first published online: 9 FEB 2016 | DOI: 10.1111/dgd.12264

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    Male somatic cells – expressing red fluorescence protein (RFP) – were fused with immature oocytes, matured in vitro and then parthenogenetically activated. We obtained RFP-expressing embryos indicating the injected somatic cells genetic materials were incorporated and functioned. This approach would be a step forward to treat sterility but needs further improvement.

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    Time in Development (pages 3–5)

    Carina Hanashima, Takashi Nishimura, Harukazu Nakamura and Claudio D. Stern

    Article first published online: 28 JAN 2016 | DOI: 10.1111/dgd.12265

  5. Intermittent hydrostatic pressure maintains and enhances the chondrogenic differentiation of cartilage progenitor cells cultivated in alginate beads

    Yang Li, Jianxin Zhou, Xiaofei Yang, Yiqiu Jiang and Jianchao Gui

    Article first published online: 15 JAN 2016 | DOI: 10.1111/dgd.12261

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    This experiment mainly explores the effects of intermittent hydrostatic pressure (IHP) on chondrogenic differentiation of cartilage progenitor cells cultivated in alginate beads and finds the superiority of this newly rising research object among a number of source cells candidates with IHP.

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