Development, Growth & Differentiation

Cover image for Vol. 58 Issue 7

Edited By: Harukazu Nakamura

Impact Factor: 2.412

ISI Journal Citation Reports © Ranking: 2015: 20/41 (Developmental Biology); 128/187 (Cell Biology)

Online ISSN: 1440-169X

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  1. Paraptosis-like cell death in Wistar rat granulosa cells

    Nayeli Torres-Ramírez, María L. Escobar, Gerardo H. Vázquez-Nin, Rosario Ortiz and Olga M. Echeverría

    Version of Record online: 29 SEP 2016 | DOI: 10.1111/dgd.12322

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    The follicular atresia is a normal process in mammals' ovaries. Along this process the non-selected oocytes to be ovulated are degraded. It has been described that during atresia, the apoptosis has been identified as the main route of elimination; however, some ultrastructural characteristics observed in the granulosa cells during the atretic process are not corresponding to the classical apoptotic process. In this work we have evidenced that those altered morphology is corresponding with the paraptosis process, which is characterized by the endoplasmic reticulum being swollen, no presence of active caspase-3, and the fragmented chromatin is absent.

  2. Optimizing neuronal differentiation of human pluripotent NT2 stem cells in monolayer cultures

    Sepideh Abolpour Mofrad, Katharina Kuenzel, Oliver Friedrich and Daniel F. Gilbert

    Version of Record online: 26 SEP 2016 | DOI: 10.1111/dgd.12323

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    We have compared two almost similar monolayer culture protocols and one rather different suspension culture approach for differentiation of NT2 cells into neuronal cells using efficiency, i.e. cell number, cluster number and size, as well as the expression of CNS specific markers (including GFAP and MAP2) as quantifiers. Based on the results of our comparative analysis, we have established a refined method for differentiation of NT2 cells in monolayer cultures. We visualize expression of neuronal proteins in NT2-N cells using monoclonal antibodies raised against the markers beta-III-tubulin and Synapsin I and validate the robustness of the method by quantifying cluster number in a dataset generated from multiple replicate experiments conducted during a period of 8 months.

  3. Mitochondrial trafficking through Rhot1 is involved in the aggregation of germinal granule components during primordial germ cell formation in Xenopus embryos

    Haru Tada, Yuya Taira, Keisuke Morichika and Tsutomu Kinoshita

    Version of Record online: 1 SEP 2016 | DOI: 10.1111/dgd.12310

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    Using Xenopus transgenic line with EGFP-labeled mitochondria and truncated form of mitochondria-anchor protein, we demonstrate that mitochondrial GTPase Rhot1 is essential for the aggregation of mitochondria and germinal granule components in germ plasm. Our results suggest that mitochondrial trafficking is involved in the aggregation of germinal granule components, which are essential for primordial germ cells formation.

  4. Molecular markers for X-ray-insensitive differentiated cells in the Inner and outer regions of the mesenchymal space in planarian Dugesia japonica (pages 609–619)

    Machiko Teramoto, Tomomi Kudome-Takamatsu, Osamu Nishimura, Yang An, Makoto Kashima, Norito Shibata and Kiyokazu Agata

    Version of Record online: 17 AUG 2016 | DOI: 10.1111/dgd.12309

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    Planarian mesenchymal space can be divided into two regions using marker genes for both X-ray-sensitive cells and differentiated cells. These findings support the idea that the mesenchymal space may have a key role in regulation of the stem cell system in planarians.

  5. Human pancreatic islets develop through fusion of distinct β and α/δ islets

    Inchul Lee

    Version of Record online: 17 AUG 2016 | DOI: 10.1111/dgd.12308

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    Fetal pancreatic islets develop first in two distinct types consisting of β or α/δ cells, respectively. During the development, they fuse with each other forming the prototype of mature human islets.

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