3D Electrospun scaffolds promote a cytotrophic phenotype of cultured primary astrocytes
Chew L. Lau, Michelle Kovacevic, Tine S. Tingleff, John S. Forsythe, Holly S. Cate, Daniel Merlo, Cecilia Cederfur, Francesca L. Maclean, Clare L. Parish, Malcolm K. Horne, David R. Nisbet and Philip M. Beart
Article first published online: 9 APR 2014 | DOI: 10.1111/jnc.12702
Astrocytes exist in phenotypes with pro-survival and destructive components, and their biology can be modulated by changing phenotype. Our findings demonstrate murine astrocytes adopt a healthy phenotype when cultured in 3D. Astrocytes proliferate and extend into poly-ε-caprolactone scaffolds displaying 3D stellated morphologies with reduced GFAP expression and actin stress fibres, plus a cytotrophic gene profile. Bioengineered 3D scaffolds have potential to direct inflammation to aid regenerative neurobiology.