Letters in Applied Microbiology

Cover image for Vol. 63 Issue 4

Early View (Online Version of Record published before inclusion in an issue)

Edited By: J.-Y. Maillard

Impact Factor: 1.579

ISI Journal Citation Reports © Ranking: 2015: 96/123 (Microbiology); 106/161 (Biotechnology & Applied Microbiology)

Online ISSN: 1472-765X

Associated Title(s): Journal of Applied Microbiology

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  1. Original Articles

    1. Detecting a novel Eriocheir sinensis reovirus by reverse transcription loop-mediated isothermal amplification assay

      Y. Ma, T. Dai, A. Serwadda and H. Shen

      Version of Record online: 15 SEP 2016 | DOI: 10.1111/lam.12630

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      Significance and Impact of the Study: Tremor disease (TD) is one of the most serious diseases of Eriocheir sinensis. A novel E. sinensis reovirus (EsRV) was identified from E. sinensis afflicted with TD and caused high mortality. We developed a reverse transcription loop-mediated isothermal amplification assay with high specificity, sensitivity and rapidity to detect EsRV, which can be used to diagnose aquatic animal diseases, particularly where expensive diagnostic instruments are not available.

    2. Fluorescence in situ hybridization of Microcystis strains producing microcystin using specific mRNA probes

      P. Zeller, A. Méjean, I. Biegala, V. Contremoulins and O. Ploux

      Version of Record online: 14 SEP 2016 | DOI: 10.1111/lam.12634

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      Significance and Impact of the Study: Cyanobacteria producing toxic compounds (cyanotoxins) are present in the environment and in water bodies. Their presence poses a threat on human and animal health. It is thus important to detect, identify and enumerate these toxic Cyanobacteria. Using tyramide signal amplification fluorescence in situ hybridization (TSA-FISH) and specific probes, with confocal laser scanning microscopy, we have specifically detected Microcystis strains producing microcystin toxins. The data presented here might be applied to the monitoring of water bodies at early stages and all along the formation of Microcystis blooms.

    3. Impact of enumeration method on diversity of Escherichia coli genotypes isolated from surface water

      E.C. Martin and T.J. Gentry

      Version of Record online: 14 SEP 2016 | DOI: 10.1111/lam.12633

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      Significance and Impact of the Study: This study characterized the impact of three USEPA-approved Escherichia coli enumeration methods on observed E. coli population diversity in surface water samples. Results indicated that these methods produced similar E. coli enumeration data but were more variable in the diversity of E. coli genotypes observed. Although the three methods enumerate the same species, differences in media composition, growth platform, and incubation temperature likely contribute to the selection of different cultivable populations of E. coli, and thus caution should be used when implementing these methods interchangeably for downstream applications which require cultivated isolates.

    4. High-throughput identification of the microbial biodiversity of cocoa bean fermentation by MALDI-TOF MS

      S. Miescher Schwenninger, S. Freimüller Leischtfeld and C. Gantenbein-Demarchi

      Version of Record online: 4 SEP 2016 | DOI: 10.1111/lam.12621

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      Significance and Impact of the Study: Microbial identification with MALDI-TOF MS has increased the number of samples that can be analysed in a given time, a prerequisite for high-throughput methods. This method is already widely used for the identification of clinical microbial isolates, whereas in food fermentation research, including cocoa bean fermentation, microbiota is mostly identified by time-consuming, biochemical-based phenotyping and molecular approaches. This study presents the use of MALDI-TOF MS for characterizing the microbial biodiversity of cocoa bean fermentation. The feasibility of MALDI-TOF MS identification of cocoa-specific microbiota has been shown with samples collected during on-site studies in two countries of origin, Bolivia and Brazil.

    5. Rapid and accurate identification of Xanthomonas citri subspecies citri by fluorescence in situ hybridization

      D.W. Waite, R. Griffin, R. Taylor and S. George

      Version of Record online: 4 SEP 2016 | DOI: 10.1111/lam.12624

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      Significance and Impact of the Study: Xanthomonas citri subsp. citri (Xcc) is an aggressive and hardy pathogen of citrus plants worldwide. Outbreaks are difficult and costly to contain and the establishment of citrus canker results in restricted trade. In order to extend the existing toolkit for identification of Xcc we developed a novel diagnostic approach based on fluorescence in situ hybridization. Our approach is of comparable specificity and sensitivity to existing methods but can be performed directly on infected tissue making it significantly faster than existing PCRs, and requiring fewer laboratory resources.

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