Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle
Z. Li, H. Huang, H. Zhao, K. Meng, J. Zhao, P. Shi, P. Yang, H. Luo, Y. Wang and B. Yao
Article first published online: 13 OCT 2014 | DOI: 10.1111/lam.12318
Significance and Impact of the Study: Ruminal phytases, that are cysteine phytases, are novel in sequences and functions. Great divergence in the constitution and abundance of cysteine phytase genes at the genome and transcriptional levels suggested that transcript data are more reliable to reflect the information of functional genes. Phylogenetic and rarefaction analyses indicated that the cysteine phytase genes from uncultured bacteria instead of Firmicutes play the major phytate-degrading role in rumen, and their constitution is dynamic at different time points. This study provides a new insight into ruminal cysteine phytase genes and undermines their expression profiles over a whole feeding cycle.