Cover image for Vol. 57 Issue 2

Edited By: Paul M. Ness

Impact Factor: 3.042

ISI Journal Citation Reports © Ranking: 2015: 30/70 (Hematology)

Online ISSN: 1537-2995

Author Guidelines

Author Guidelines

Author Guidelines


TRANSFUSION, the journal of the AABB is the foremost publication in the world for new information regarding transfusion medicine.

TRANSFUSION reports on the latest technical advances, discusses opposing viewpoints regarding controversial issues, and presents key conference proceedings. In addition to blood banking and transfusion medicine topics, TRANSFUSION presents submissions concerning tissue transplantation and hematopoietic, cellular, and gene therapies. Acceptance of papers for publication is based on merit; equal consideration will be given to papers submitted by nonmembers and members of the Association.

Manuscripts are considered for publication on the condition that they are submitted solely to TRANSFUSION. Except for abstracts of 500 words or fewer that are published in connection with scientific meetings, no substantial part of the paper may have been submitted for publication elsewhere.


Manuscripts must be submitted online, at Complete, detailed instructions on up-loading your manuscript are available at this website. Any major word processor software may be used, and both DOS-based and Macintosh operating systems are acceptable. In addition, the Conflict of Interest Form and the Submission Fee Form authorizing payment of the $50.00 Manuscript submission fee should be sent to:

Mary Ann Reese
Assistant Editor, TRANSFUSION
The Johns Hopkins University
550 Building/Suite 810
550 North Broadway
Baltimore, MD 21205
Phone: (443) 287-3696; Fax: (443) 287-3900

These forms are available in your Author Center of Manuscript Central online submission system under Instructions and Forms. Please put the manuscript ID number on all forms, which you will receive via email after you have submitted your manuscript. Keep a copy of all materials submitted. Please note on your TITLE PAGE the CONFLICTS OF INTEREST of any of the authors. IF THERE ARE NO CONFLICTS, PLEASE STATE: The authors declare that they have no conflicts of interest relevant to the manuscript submitted to TRANSFUSION. Every manuscript submitted for review must have a statement on the TITLE PAGE acknowledging any conflicts or their absence. Failure to provide this statement will result in the unsubmission of your manuscript from the review process and a resubmission will be sent for review only after the required statement is completed.

OnlineOpen is available to authors of primary research articles who wish to make their article available to non-subscribers on publication, or whose funding agency requires grantees to archive the final version of their article. With OnlineOpen the author, the author's funding agency, or the author's institution pays a fee to ensure that the article is made available to non-subscribers upon publication via Wiley Online Library, as well as deposited in the funding agency's preferred archive. For the full list of terms and conditions see

Any authors wishing to send their paper OnlineOpen will be required to complete the payment form available from our website at:

Prior to acceptance there is no requirement to inform an Editorial Office that you intend to publish your paper OnlineOpen if you do not wish. All OnlineOpen articles are treated in the same way as any other article. They go through the journal's standard peer-review process and will be accepted or rejected based on their own merit.

Processing and Review of Manuscripts. The Editor will provide the author with the name and address of the Associate Editor responsible for the review. Further review by members of the editorial board or other experts will occur. The review process takes about 6 weeks, and most manuscripts require revision before final acceptance.

Proofs and Publication of Manuscripts. All manuscripts are subject to editing for consistency of style by the Editor, the publisher, or both. Authors will be sent page proofs and a query sheet by the publisher. Only important changes in data are allowed. The page proofs and answered query sheet should be returned directly to the publisher by email or fax within 24 hours of receipt to allow for accommodation of authors’ corrections and to avoid publication delay. Manuscripts generally will be published in the order that they are accepted. Keep a copy of the corrected galley pages.

Corrections, Retractions of Research Findings. Errors may occur during the publication process. The Editor will discuss these errors with the senior author and make a decision regarding publication of a correction. If authors discover an error in their work after publication, this should be discussed with the Editor, who will make a decision regarding publication of a correction.

If a paper containing fraudulent data has been published, the journal will print a retraction. The retraction will be listed in the contents page, will include in its heading the title of the original article, and in its text will explain why the article is being retracted. The Editor may also choose to publish an announcement to the effect that the validity of previously published work is not ensured.

Press Releases. On occasion, TRANSFUSION may wish to feature a report in a release to the press to coincide with publication. All press releases are subject to final approval by the Editor. The senior author will be notified prior to release. If the author feels a need in exceptional cases to publicize data in advance of publication, the Editor must be consulted.


Manuscripts must be prepared in accordance with the following instructions to authors. These instructions are based on the Uniform Requirements for Manuscripts Submitted to Biomedical Journals (also known as the Declaration of Vancouver). For more information, refer to International Committee of Medical Journal Editors. Uniform requirements for manuscripts submitted to biomedical journals. Ann Intern Med 1997;126:36–47. Manuscripts reporting the results of a randomized controlled trial should be prepared in accordance with the CONSORT Guidelines. Authors should consult the CONSORT Checklist for specific requirements. The checklists can be found at the following websites: (parallel arm randomized controlled trial design); or (other clinical trial designs). Trial registration number and site should be included in the Methods section of your Manuscript.


Manuscripts should be submitted online, at Keep a copy of everything submitted.

The entire article – 1) title page; 2) abstract and key words; 3) text; 4) acknowledgments; 5) references; 6) figure legends; 7) tables and legends; 8) appendices – should be saved in a single file; only electronic figures should be supplied as separate files.

Authors might want to visit the Wiley-Blackwell website for authors at which details further information on the preparation and submission of articles and figures. Please note that Word 2007 is not yet compatible with journal production systems.


Transfusion Clips
TRANSFUSION CLIPS is a new section which will feature short movie clips. Authors will submit their work through Manuscript Central using the “Transfusion Clip” manuscript type. The clip should ideally be shorter than 5 minutes and never exceed 10 minutes. Please prepare a one-page Title page with author(s), affiliation, contact information and Conflict of Interest statement; a brief paragraph of no more than 250 words describing the clip; and one representative stationary picture which can be, but does not have to be, taken from the video. This will be uploaded in the online system, as well as the video clip (in MP4, QT, WMV, AVI or MPEG format). All video clips must be created with commonly-used codecs, and the codec used should be noted in the supporting information legend. Video files should be tested for playback before submission, preferably on computers not used for its creation, to check for any compatibility issues. Wiley-Blackwell will not host codec files, or be responsible for supporting video supporting information where the codec used is non-standard. Please use the File Designation WORD DOCUMENT for the title Page and brief description; and SUPPLEMENTAL FILE for the video clip. The brief description should be placed into the Abstract text box section as well. An accepted clip will be assigned one page in the print journal containing the uploaded information (author(s), affiliation, brief description etc.) plus instructions for locating and viewing the video.

Blood Group Genomics
This section accepts manuscripts covering the molecular biology of RBC’s, WBC’s, and platelets. These include full-length original articles, brief reports (see below) and single page reports of new alleles describing the genetic basis of antigenic polymorphisms of clinical relevance and interest to Transfusion readership.  Topics also include new assays and the application of genomic science to the practice of transfusion medicine. Please contact Connie Westhoff, PhD ( with further submission requirement questions.

1. Use accepted nucleotide, amino acid, and allele notation and symbols as defined by the ISBT and available on the ISBT Working Party on Red Cell Immunogenetics and Terminology website.  Use c. to indicate the position of the nucleotide change in the mRNA, and p. to modify the amino acid change in the protein.  For example , c.574C>T, p.Arg192Trp.

2. cDNA sequencing or full gene sequencing is required to associated a specific polymorphism with an observed phenotypic change and to define a new allele.

3. Nucleotide(s) change(s) identified by cDNA analysis should be confirmed in genomic DNA (by sequencing, PCR-RFLP, SS-PCR).

4. Accession numbers of new or novel sequences should be provided by depositing in an appropriate database (GenBank/EMBL).

5. Web-based resources used (database, online computer program, etc.) should be given along with the URL in a separate section entitled “Web Resources”, following the Acknowledgments. URLs should not to be included in the text as these can change. The date the URL was accessed should also be included.


One-Page Reports of New Alleles and/or Antigens (guidelines above also apply)

1.      Length: one published page, or less. Contained within the single published page

            Word count: 600 or less 

            One table (or figure)

            Maximum 5 references

2.       Provide a title and the author’s(s’) name(s), degree(s), and institution (s), and source(s) of support in the form of grants or equipment, and CONFLICT OF INTEREST form.

3.      The sequence must be deposited in an appropriate database (GenBank/EMBL) and the accession number provided.

4.      The SNP database should be consulted and the reference number provided if one is available.

5.      An official ISBT allele and/or antigen designation should be provided after the manuscript has undergone peer review.

6.       Suggested format: 

            Background or Case Report

            Brief Methods

            Results (table format is acceptable)

            Ethnicity; prevalence or allele frequency

            Brief Summary



Review articles are not to exceed 4,000 words excluding the abstract, references, tables and illustrations.

Transfusion Medicine Illustrated
Pictures, line drawings, and other visuals (no more than 3) and a brief explanation (no more than 250 words) that together capture a unique aspect of transfusion medicine. The text must include a brief title, the full name and affiliation of each author, plus the name, address, telephone, fax and e-mail address of the corresponding author. Manuscripts should be submitted online, at A Conflict of Interest Form should be completed for each submission and sent to Mary Ann Reese at the address given previously. There is no submission fee for TMI’s.

Transfusion Medicine History Illustrated
TRANSFUSIONis soliciting the submission of unique pictorial material for Transfusion Medicine History Illustrated that will highlight our past, explain our present and suggest the future. Pictures, drawings, and other visuals (no more than 3) and a brief explanation (no more than 300 words) ca be submitted online at The text must include a title, the full name and affiliation of each author, plus the name, address, telephone, fax and e-mail address of the corresponding author. All submission forms should be sent to Mary Ann Reese at the address given previously. There is no submission fee for TMHIs.

Case Reports
Case Reports should include an Abstract, Case Presentation, Discussion, and Conclusion. Manuscript preparation should follow the guidelines of original research manuscripts.

Brief Reports
Brief Reports should follow the guidelines for original research manuscript preparation, with a length of four to ten double-spaced typed pages. They should have a maximum of three figures and or tables, maximum 25 references, and an abstract length not exceeding 250 words.

How Do I ...
The How Do I...section is designed to publish expert opinions in areas of transfusion medicine, blood banking, coagulation/hemostasis and cellular therapies related to topics for which practice, policy or understanding are unclear. All "How Do I ..." submissions should be submitted through Manuscript Central with "How Do I ..." at the beginning of the title. Use Brief Report for the Manuscript Type. Please contact Beth Shaz, MD ( with questions regarding submission of manuscripts to this section.

Clinical Research Focus
This feature contains short articles focusing on a specific aspect of clinical research methodology. Please contact Nancy M. Heddle ( for submission requirements.

Letters to the Editor
A title must be provided for each letter. Letters should not be longer than 2 double-spaced pages and have no more than 5 references. Tables, figures, and illustrations should be limited to a total of 2. The closing should include the author’s(s’) name(s), degree(s), and address(es) but not professional appointments. Letters to the Editor must be submitted online, at Choose LETTERS as the Manuscript Type. Conflict of Interest forms can be emailed or faxed to the Editorial Office. Please place a CONFLICT OF INTEREST STATEMENT either on the Title Page or after the Author closing. If there is no Conflict of Interest, please state that fact. There is no Submission Fee for a Letter.


Research articles are not to exceed 4,000 words, excluding the abstract, references and illustrations. Do not use justified right margins. Begin each of the following sections on a separate page: title page, abstract and key words, text, acknowledgments, references, individual tables, illustrations, and legends. All pages should be saved in a single file, with the exception of figure files. Do not use Registered Trademark symbols, Trademark symbols, Headers, Footers (including page numbers), nor Footnotes in your manuscript. Double Space (2.0) the body of your manuscript.

Title Page
The title page should contain: 1) the title of the article, which should be concise but informative and should not include abbreviations or brand names; 2) a short running head of no more than 40 characters (count letters and spaces) typed at the bottom of the title page and identified as such; 3) first and last names of each author but not his/her titles, degrees, positions, or academic ranks. Attribution for the work should be stated separately (see below); 4) the name(s) of department(s) and institution(s) to which the work should be attributed; 5) disclaimers, if any; 6) name, address, telephone and FAX numbers, and e-mail address of the author responsible for correspondence about the manuscript; 7) either the name and address of the author responsible for reprint requests or a statement that reprints will not be available from the author; and 8) the source(s) of support in the form of grants, equipment, or drugs; 9) CONFLICT OF INTEREST. Please note on the Title Page of your word document all conflicts of interest of any of the authors. If there are no conflicts, please state that fact; 10) No BRAND NAMES in the Title; and no registered trademark symbols throughout the manuscript; 11) Please include the WORD COUNT.

The second page must carry an abstract of not more than 250 words. The abstract should consist of four sections labeled BACKGROUND, STUDY DESIGN AND METHODS, RESULTS, and CONCLUSION. These sections should briefly describe, respectively, the objectives of the study or investigation, the basic procedures (study subjects and analytic methods), the main findings (giving specific data and the statistical significance, if possible), and the principal conclusions. Emphasize new and important aspects of the study. Do not use first-person pronouns in the abstract. Key words should follow the Abstract on the same page.

The text of observational and experimental articles is usually divided into sections using the headings INTRODUCTION, MATERIALS AND METHODS, RESULTS, and DISCUSSION. Long articles may need subheadings within some sections to clarify content, especially in the Materials and Methods, Results, and Discussion sections. Other types of articles, such as reviews and editorials, may require other formats; authors should consult the Editor for guidance. Use as much active voice as possible in writing.

Introduction. Clearly state the purpose of the article. Summarize the rationale used for the study or observations. Give onlypertinent references: do not review the subject extensively. Do not include data or conclusions from work being reported.

Case Report. Include only for manuscripts that focus on individual histories.

Materials and Methods. Describe your selection of observational or experimental subjects (patients or animals, including controls, and the number in each study group) clearly. Identify the methods, apparatus, equipment, reagents, and procedures used in sufficient detail to allow other colleagues to reproduce the results. Publication of an original research article implies that the authors are prepared to distribute freely to academic researchers for their own use any materials (e.g., cell lines, DNA clones, antibodies) used in the experiments. Provide model name or number, and the manufacturer’s name (in parentheses) the first time a reagent, an apparatus, or any equipment is mentioned. Give references to established methods; provide references and brief descriptions of methods that are not well-known; describe new or substantially modified methods, give reasons for using them, and describe their limitations. Identify precisely all drugs and chemicals used, including generic name(s), dose(s), and route(s) of administration.
When reporting experiments conducted on humans, indicate whether the procedures followed were in accordance with the ethical standards of the committee(s) on human experimentation of the institution(s) in which the experiments took place. Details that might identify patients should be avoided unless essential for scientific purposes. If identification of patients is unavoidable, their informed consent should be obtained.
When reporting experiments conducted on animals, indicate whether the institution’s or the National Research Council’s guide for the care and use of laboratory animals was followed. All manuscripts dealing with recombinant DNA research must indicate the physical and biologic containment procedures practiced, in accordance with the Health Guidelines for Research Involving Recombinant DNA Molecules established by the National Institutes of Health.

Statistics. Describe statistical methods in enough detail to enable a knowledgeable reader with access to the original data to verify the reported results. When possible, quantify findings and present them with appropriate indicators of measurement error or uncertainty (such as confidence intervals). Avoid sole reliance on statistical hypothesis testing, such as the use of p values, which fails to convey important quantitative information. Discuss the eligibility of experimental subjects. Give details about randomization. Describe the methods for and success of any blinding of observations. Report treatment complications. Specify the numbers of observations. Report losses to observation (such as dropouts from a clinical trial). References for study design and statistical methods should be to standard works (with pages stated) when possible, rather than to papers in which designs or methods were originally reported. Specify any general-use computer programs used.
Put general descriptions of methods in the Methods section. Avoid nontechnical uses of technical terms in statistics, such as ‘random’ (which implies a randomizing device), ‘normal’, ‘significant’, ‘correlation’, and ‘sample’. Define statistical terms, abbreviations, and most symbols.

Results. Present your results in logical sequence in the text, tables, and/or illustrations. Do not repeat in the text any data presented in tables and/or illustrations; emphasize or summarize only important observations, and avoid tables displaying data showing insignificant differences among groups.

Discussion. Emphasize the new and important aspects of the study and the conclusions that follow from them. Do not repeat in detail the data given in the Results section or in tables or illustrations. Include in the Discussion the implications of the findings and their limitations, and relate the observations to other relevant studies. Link the conclusions with the stated purpose of the study, but avoid unqualified statements and conclusions not completely supported by the data presented. Avoid claiming priority and alluding to work that has not been completed. State new hypotheses when warranted, but clearly label them as such. Recommendations, when appropriate, may be included.

Acknowledgments include one or more statements that specify:1) contributions that should be acknowledged but do not justify authorship, such as general support by a departmental chairman; 2) technical help received; 3) financial and material support, specifying the nature of the support.
Persons who have contributed intellectually to the paper, but whose contributions do not justify authorship, may be named and their function or contribution described as, for example, ‘scientific adviser’, ‘critical review of study proposal’, ‘data collection’, or ‘participation in clinical trial’. Such persons must have given written permission to be named. Authors are responsible for obtaining this written permission from persons acknowledged by name, because readers may infer their endorsement of the data and conclusions.
Technical help should be acknowledged in a paragraph separate from paragraphs acknowledging other contributions.

Number the references consecutively in the order in which they are first mentioned in the text. Identify references in text, tables, and legends by Arabic numerals superscripted (after any necessary punctuation). References cited only in tables or legends should be numbered in accordance with a sequence established by the first mention in the text of the table or illustration.
Please include the first three listed authors, et al. in the Reference List.
Try to avoid using abstracts as references; ‘unpublished observations’ and ‘personal communications’ may not be used as references, although references to written, not oral, communications may be inserted (in parentheses) in the text. Give the name of the person from whom the communication was received and the date (month and year) of the communication. References to manuscripts accepted but not yet published may be included; designate the journal name, followed by ‘in press’ (in parentheses).Information from manuscripts submitted but not yet accepted should be cited in the text as ‘unpublished observations’ or ‘submitted for publication’ (in parentheses).
References must be verified by the author(s) against the original documents. Inclusive page numbers of articles are required. Authors may be asked to provide a copy of the document if necessary for verification.

Type each table double-spaced on a separate sheet. Do not submit photographs of tables. Number tables consecutively and provide a brief title for each. Give each column a short or abbreviated heading. Place explanatory information in footnotes, not in the headings. For footnotes, use the following symbols in this sequence: *, †, ‡, §, ||, ¶, **, ††, etc. Identify statistical measures of variations such as SD and SEM. Omit internal horizontal and vertical rules (lines).
In general, a small table covers one column and is approximately 3.25 in or 80 mm wide and should not have more than four columns. For a large table that is to occupy the full width of a page, use no more than 8 to 10 columns over a width of approximately 6.7 in or 170 mm. In the text, cite each table in consecutive order.
If data from another published or unpublished source are used, obtain written permission to publish the data in TRANSFUSION. Enclose a copy of the permission when submitting the manuscript, and acknowledge the source fully. Some sources charge a fee to reprint information. It is the author’s responsibility to pay any fees associated with publishing data from another source. TRANSFUSION does not pay for permission to reprint material, as it does not charge a fee for granting permission to use TRANSFUSION-copyrighted material.

In general, one table/figure per 500 words of text is acceptable. Tables that restate data presented in either the text or figures will not be accepted. The Editor may recommend that tables containing important backup data too extensive to be published may be made available online only as TRANSFUSION supplementary material. In that event, an appropriate statement will be added to the text.

Cover Illustrations
If you have a color picture you’d like us to consider for the cover, please submit it in one of the following ways: color print, slide, or separated film. If you are creating a computer color graphic, please save the file in either TIFF or EPS format with a minimum resolution of 300 d.p.i. at final size. The CMYK color process should be used in preference to RGB. Send the file and color proof to the Editor.

No more than 1 figure/table per 500 words, maximum 8. Large figures with several sections (1A, 1B, 1C, etc.) may be considered Supplemental data if the numbers are more than 8.

Illustrations include figures, photomicrographs, and photographs. There are three preferred formats for digital artwork submission: Encapsulated PostScript (EPS), Portable Document Format (PDF) and Tagged Image Format (TIFF). We suggest that line art be saved as EPS files. Alternately, these may be saved as PDF files at 600 dots per inch (dpi) or better at final size. Tone art, or photographic images, should be saved as TIFF files with a resolution of 300 dpi at final size. For combination figures, or artwork that contains both photographs and labeling. We recommend saving figures as EPS files, or as PDF files with a resolution of 600 dpi or better at final size. More detailed information on the submission of electronic artwork can be found at:

Titles for illustrations belong in the legends for illustrations, not on the illustrations themselves. If there are more than two figures, please individually number, using Arabic numbers. Photomicrographs must have internal scale markers, and the magnification must be stated. Symbols, arrows, or letters used in the photomicrographs should contrast with the background.
If any person is identifiable in a photograph, written permission from the subject(s) to use the photograph must accompany the manuscript.
Cite each figure in the text in consecutive order. If a figure has been published, acknowledge the original source and submit with the manuscript written permission from the copyright holder (usually the publisher or journal) to reproduce the material. Permission is required, even for one's own publications, except for documents in the public domain.

Photographs in color, approved by the Editor, are published at the author's expense. Color reproduction is $700 per figure.

Legends for Illustrations
Type legends for illustrations double-spaced, starting on a separate page, with Arabic numbers corresponding to the illustration numbers. When symbols, arrows, numbers, or letters are used to identify parts of illustrations, identify and explain each one in the legends. Explain internal scales and identify methods of preparation and staining of material that are illustrated in photomicrographs.


Blood group terminology must follow the conventions an style described by PD Issitt and MC Crookston in 'Blood group terminology: current conventions' (Transfusion 1984;24:2-7).

Nomenclature of platelet-specific antigens should follow the conventions outlined in Metcalfe P, Watkins NA, Ouwehand WH, Kaplan C, Newman P, Kekomaki R, de Haas M, Aster R, Shibata, Y, Smith J, Kiefel V, Santosa S. Nomenclature of human platelet antigens. Vox Sang 2003;85:240-245. AABB. Standards for molecular testing for red cell, platelet, and neutrophil antigens. 1st ed. Bethesda, MD: AABB, 2008. Nomenclature of red cell antigens and genes should follow the conventions outlined in Garratty, G. Dzik W, Issitt PD, et. al. Terminology for blood group antigens and genes--historical origins and guidelines in the new millennium. Transfusion 2000;40:477-89.


Measurements of length, height, weight, and volume should be reported in metric units (meter [m], kilogram [kg], liter [L]) or their decimal multiples. However, body weight may be reported in either kilograms (kg) or pounds (lb).
Temperatures should be given in degrees Celsius. Blood pressure can be given in either torr or mmHg.


The full term for which an abbreviation stands must precede its first use in the text for ALL abbreviations with the exception of units of measurement. Consult the following sources for additional standard abbreviations: 1) CSE Style Manual Committee. Scientific Style and Format: The CSE Manual for Authors, Editors, and Publishers. 7th ed. June, 2006. 2) O'Conner M, Woodford FP. Writing scientific papers in English: an ELSE-Ciba foundation guide for authors. Amsterdam, Oxford, New York: elsevier-Excerpta Medica, 1975; and 3) Day RA, Gaster B (2006) How to Write and Publish a Scientific Paper (6th Edition). Cambridge University Press, Cambridge.
No abbreviations should be used in the title of the manuscript.