© John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze
Online ISSN: 1600-0854
ORIGINAL ARTICLE: Phosphatidylinositol 3,5-Bisphosphate-Rich Membrane Domains in Endosomes and Lysosomes
ORIGINAL ARTICLE: The Sec1/Munc18 Protein Groove Plays a Conserved Role in Interaction with Sec9p/SNAP-25
ORIGINAL ARTICLE: Phosphorylation of αSNAP is Required for Secretory Organelle Biogenesis in Toxoplasma gondii
REVIEW: The Crossroads of Synaptic Growth Signaling, Membrane Traffic and Neurological Disease: Insights from Drosophila
Recently Published Issues
Recently Published Articles
- The HOPS/Class C Vps Complex Tethers High-Curvature Membranes via a Direct Protein–Membrane Interaction
Ruoya Ho and Christopher Stroupe
Version of Record online: 15 JUL 2016 | DOI: 10.1111/tra.12421
Here, we show that the HOPS membrane tethering complex can tether highly curved membranes by binding to these membranes via the amphipathic lipid packing sensor (ALPS) motif in its Vps41p subunit. We propose that this protein–membrane interaction directs the localization of HOPS to the highly curved ‘vertex ring’ at the edge of the flattened zone of contact between tethered yeast vacuoles.
- Vesicles are persistent features of different plastids
Emelie Lindquist, Katalin Solymosi and Henrik Aronsson
Accepted manuscript online: 12 JUL 2016 09:40PM EST | DOI: 10.1111/tra.12427
Vesicles in plastids have been observed repeatedly, suggested to function in thylakoid biogenesis. Previous observations have mainly concerned proplastids and chloroplasts, often being pre-treated to induce formation or inhibit fusion of vesicles. Here we present vesicle-like structures in etio-, etio-chloro-, leuco-, chromo- and desiccoplasts, in addition to both proplastids and chloroplasts. They are here shown without any pre-treatment of plants aiming to enhance vesicle appearance, and in different species (including both C3 and C4 plants), cell types and organs.
- Rab11 Regulates the Mast Cell Exocytic Response
Joshua D. Wilson, Sarah A. Shelby, David Holowka and Barbara Baird
Version of Record online: 11 JUL 2016 | DOI: 10.1111/tra.12418
Mast cell exocytosis causes allergy through the release of mediators from secretory lysosomes; costimulated exocytosis of recycling endosomes (REs) provides a source of additional membrane and may play other roles. Here, we show that a dominant negative form of the RE protein Rab11 (S25N) interferes with both of these exocytic processes. Inhibition mediated by S25N Rab11 can be bypassed using compounds that block actin polymerization. Furthermore, inhibition of stimulated exocytosis by the F-actin stabilizer, jasplakinolide, is not additive with S25N Rab11, implicating Rab11 in actin remodeling necessary for exocytosis.
- BMP2 Transfer to Neighboring Cells and Activation of Signaling
Hamed Alborzinia, Marjan Shaikhkarami, Peter Hortschansky and Stefan Wölfl
Version of Record online: 10 JUL 2016 | DOI: 10.1111/tra.12420
BMP signaling plays a central role in development and organ homeostasis, and is tightly regulated at various levels. Internalization of BMP2 by cells can provide another layer of regulation. We show that internalized BMP2 is transferred to neighboring cells and can induce BMP signaling. Cell–cell contact increased transfer and enhanced signaling. Noggin not only blocked signaling but also enhanced transfer. Inhibition of vesicular transport blocked transfer without clear interference with signaling, suggesting that BMP2 signaling occurs independent of internalization and transfer.
- Identification of new fungal peroxisomal matrix proteins and revision of the PTS1 consensus
Christopher Nötzel, Thomas Lingner, Heiner Klingenberg and Sven Thoms
Accepted manuscript online: 8 JUL 2016 08:30AM EST | DOI: 10.1111/tra.12426
The peroxisomal targeting signal type 1 (PTS1) is the prevalent peroxisomal targeting signal. Its current definition, however, is largely incomplete. We have modelled PTS1 by a machine learning approach in yeast. Based on this, we identified two conserved genes encoding novel peroxisomal proteins and we updated the consensus motif to now include all PTS1 proteins in yeast.