Cover image for Vol. 17 Issue 11

Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze

Online ISSN: 1600-0854

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Recently Published Articles

  1. An evolutionary balance: conservation vs innovation in ciliate membrane trafficking

    Sabrice Guerrier, Helmut Plattner, Elisabeth Richardson, Joel B. Dacks and Aaron P. Turkewitz

    Version of Record online: 27 OCT 2016 | DOI: 10.1111/tra.12450

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    Several species of ciliates represent useful experiment organisms for cell biology research, and particularly for analyzing the outcomes of evolutionary trajectories that are distant from those of fungi or animals. This review focuses on 3 phenomena in ciliates that are all related to conserved pathways of endolysosomal trafficking, but with unique ciliate features: phagosome formation and maturation, regulated exocytosis of secretory granules, and programmed degradation of specific nuclei during conjugation.

  2. Cadherin tales: Regulation of cadherin function by endocytic membrane trafficking

    Chantel M. Cadwell, Wenji Su and Andrew P. Kowalczyk

    Version of Record online: 27 OCT 2016 | DOI: 10.1111/tra.12448

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    Cellular rearrangements during development, wound healing and metastasis require dynamic regulation of adhesion. Endocytic membrane trafficking has emerged as a fundamental mechanism by which cells confer plasticity to adhesive junctions. Recent studies indicate that the juxtamembrane domain of classical cadherins contains multiple endocytic motifs, or “switches,” that can be used by cellular membrane trafficking machinery to regulate adhesion. This review focuses on p120-catenin and other cadherin-binding proteins, ubiquitin ligases and growth factors as key modulators of cadherin membrane trafficking.

  3. KymoAnalyzer: A Software Tool for the Quantitative Analysis of Intracellular Transport in Neurons

    Sylvia Neumann, Romain Chassefeyre, George E. Campbell and Sandra E. Encalada

    Accepted manuscript online: 22 OCT 2016 03:55AM EST | DOI: 10.1111/tra.12456

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    KymoAnalyzer is an open-source ImageJ package designed for the analysis of the intracellular transport of fluorescently-labeled cargoes and molecular motors in neuronal projections, as well as in vitro, on isolated microtubules. This semi-automated software generates kymographs from time-lapse movies, guides the user in the manual assignment of particle trajectories from kymograph images, and automatically calculates multiple transport parameters at the single-particle level for thousands of moving particles. It allows cell biologists to perform relevant analyses from large movie datasets.

  4. Cresyl violet: a superior fluorescent lysosomal marker

    Philip P. Ostrowski, Gregory D. Fairn, Sergio Grinstein and Danielle E. Johnson

    Version of Record online: 17 OCT 2016 | DOI: 10.1111/tra.12447

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    We have identified cresyl violet as a superior fluorescent lysosomal marker. This red-shifted fluorophore accumulates selectively in lysosomes in a pH-dependent manner. Furthermore, we find that cresyl violet is remarkably photostable and does not photoconvert to other fluorescent species, unlike other widely used lysosomal probes. Cresyl violet does not alter the lysosomal buffering capacity, membrane integrity or luminal pH, it is widely available, and costs ≈30 000 times less than competing probes.

  5. 14-3-3 proteins regulate K2P5.1 surface expression on T lymphocytes

    Juncal Fernández-Orth, Petra Ehling, Tobias Ruck, Susann Pankratz, Majella-Sophie Hofmann, Peter Landgraf, Daniela C. Dieterich, Karl-Heinz Smalla, Thilo Kähne, Guiscard Seebohm, Thomas Budde, Heinz Wiendl, Stefan Bittner and Sven G. Meuth

    Accepted manuscript online: 15 OCT 2016 02:40AM EST | DOI: 10.1111/tra.12455

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    K2P5.1 channels possess a putative non-classical consensus motif for 14-3-3 proteins that mediates the interaction and promotes K2P5.1 channels to the plasma membrane. An amino acid mutation reduces the binding of 14-3-3 proteins to K2P5.1 resulting in a reduced number of channels at the plasma membrane and a decreased potassium efflux. Pharmacological inhibition of 14-3-3 protein binding to K2P5.1 functionally impacts T cell- proliferation and cytokine production. 14-3-3 proteins may represent a pharmacological target for the treatment of multiple sclerosis and other autoimmune diseases.