Cover image for Vol. 18 Issue 10

Edited By: Michael S. Marks, Trina A. Schroer, Robert G. Parton and Sharon A. Tooze

Online ISSN: 1600-0854

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Celebrating the Contributions of Ari Helenius

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Chemical and Immunological Synapses"

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Recently Published Articles

  1. MGRN1 mediated ubiquitination of α-tubulin regulates microtubule dynamics and intracellular transport

    Rukmini Mukherjee, Priyanka Majumder and Oishee Chakrabarti

    Accepted manuscript online: 13 SEP 2017 08:13AM EST | DOI: 10.1111/tra.12527

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    Microtubule-based transport is vital in neurons. Here we show that ubiquitination of α-tubulin by MGRN1 regulates microtubule polymer stability and efficiency of cargo transport on microtubule tracks. Functional loss of MGRN1 (by expression of catalytically inactive MGRN1 or presence of cytosolically exposed prion protein, CtmPrP) drastically compromises intracellular transport of mitochondria and endosomes. Hence, ubiquitination mediated microtubule dynamics is important in neurodegeneration associated with certain types of prion diseases.

  2. The dense-core vesicle maturation protein CCCP-1 binds RAB-2 and membranes through its C-terminal domain

    Jérôme Cattin-Ortolá, Irini Topalidou, Annie Dosey, Alexey J. Merz and Michael Ailion

    Version of Record online: 13 SEP 2017 | DOI: 10.1111/tra.12507

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    CCCP-1 is a coiled-coil protein important for dense-core vesicle (DCV) biogenesis. A structure-function analysis of CCCP-1 shows that its C-terminal domain is required for: (1) localization to membrane compartments near the trans-Golgi, (2) binding to activated RAB-2(3) function in DCV biogenesis and (4) direct binding to membranes. CCCP-1 has an elongated shape and forms oligomers. These findings suggest that CCCP-1 resembles members of the golgin family of proteins that act as membrane tethers.

  3. Fusion and scission of membranes: Ubiquitous topological transformations in cells

    Roland L. Knorr, Noboru Mizushima and Rumiana Dimova

    Version of Record online: 13 SEP 2017 | DOI: 10.1111/tra.12509

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    Remodeling of lipid membranes involves gradual bending as well as abrupt events: membrane fusion and membrane scission. Such discontinuous changes alter the number of individual (separate) membranes or the number of (torus-like) holes within the membrane structure: the topology of the structure changes. Here, we review cell membrane remodeling from a topological viewpoint, highlight the large number of topological changes during autophagy and link the two 2016 Nobel prizes honoring autophagy and topology.

  4. Heterogeneity in kinesin function (pages 658–671)

    Babu J.N. Reddy, Suvranta Tripathy, Michael Vershinin, Marvin E. Tanenbaum, Jing Xu, Michelle Mattson-Hoss, Karim Arabi, Dail Chapman, Tory Doolin, Changbong Hyeon and Steven P. Gross

    Version of Record online: 8 SEP 2017 | DOI: 10.1111/tra.12504

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    Kinesin motors have been studied extensively at the single-molecule level, and iIt is typically assumed that they function identically. Here we find that this assumption of homogeneous function appears incorrect: variation among motors’ velocities in vivo and in vitro is larger than the stochastic variation expected for an ensemble of “identical” motors.

  5. Golgi α1,4-fucosyltransferase of Arabidopsis thaliana partially localizes at the nuclear envelope (pages 646–657)

    Stephan Rips, Manuel Frank, Annegret Elting, Jan Niklas Offenborn and Antje von Schaewen

    Version of Record online: 5 SEP 2017 | DOI: 10.1111/tra.12506

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    The luminal domains of two homologous fucosyltransferases of Arabidopsis thaliana (At) and tomato (Le), usually residing in the trans-Golgi network (TGN) of higher plant cells, were exchanged to test for involvement of the N- and/or C-terminal domains in partial localization of the AtFucTc enzyme at the nuclear envelope (NE). Our studies show that AtFucTc likely interacts with an unknown (glyco)protein at the NE, which might either reflect chaperone binding (until heteromerization with an unknown partner) or neo-functionalization within A. thaliana.