Cover image for Vol. 17 Issue 10

Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze

Online ISSN: 1600-0854

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Recently Published Articles

  1. Characterization of a caveolin-1 mutation associated with both PAH and congenital generalized lipodystrophy

    Bing Han, Courtney A. Copeland, Yumeko Kawano, Erika Berman Rosenzweig, Eric D. Austin, Layla Shahmirzadi, Sha Tang, Krishnan Raghunathan, Wendy K. Chung and Anne K. Kenworthy

    Accepted manuscript online: 26 SEP 2016 09:10AM EST | DOI: 10.1111/tra.12452

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    We identify a heterozygous F160X mutation in the caveolin-1 (CAV1) gene in a patient with both pulmonary arterial hypertension (PAH) and congenital generalized lipodystrophy (CGL) and examine its effect on caveolae assembly. We find that the mutant protein forms hybrid caveolae together with wild type CAV1 in patient skin fibroblasts and reconstituted CAV1−/− MEFs but that these caveolae are abnormal in several respects. These caveolaer defects may serve as contributing factors to the development of PAH and CGL.

  2. Vps35-dependent recycling of Trem2 regulates microglial function

    Jie Yin, Xiaocui Liu, Qing He, Lujun Zhou, Zengqiang Yuan and Siqi Zhao

    Accepted manuscript online: 26 SEP 2016 09:10AM EST | DOI: 10.1111/tra.12451

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    Model for Vps35-mediated Trem2 recycling. Wild type Trem2 is internalized in a clathrin-dependent manner then trafficked to cell membrane through Vps35. By recycling Trem2, Vps35 is involved in suppressing pro-inflammatory responses of microglia. However, the R47H mutant Trem2 is impaired in interacting with Vps35 and accumulated in lysosome for degradation.

  3. Newly synthesized and recycling pools of the apical protein gp135 do not occupy the same compartments

    Emily H. Stoops, Michael Hull and Michael J. Caplan

    Accepted manuscript online: 20 SEP 2016 10:00AM EST | DOI: 10.1111/tra.12449

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    In polarized epithelial cells, apical or basolateral membrane proteins are sorted in endosomal compartments en route to their target membranes. We used the SNAP tag system to study the intracellular trafficking of the apical protein gp135. Newly synthesized gp135 was shown to traverse the apical recycling endosome (ARE), while post-endocytic gp135 was delivered to the early endosome (AEE) before recycling to the apical membrane. The lack of overlap in the pathways of the newly synthesized and recycling pools of gp135 may represent a mechanism for differential sorting.

  4. Cadherin Tales: Regulation of Cadherin Function by Endocytic Membrane Trafficking

    Chantel M. Cadwell, Wenji Su and Andrew P. Kowalczyk

    Accepted manuscript online: 14 SEP 2016 12:20AM EST | DOI: 10.1111/tra.12448

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    Cellular rearrangements during development, wound healing, and metastasis require dynamic regulation of adhesion. Endocytic membrane trafficking has emerged as a fundamental mechanism by which cells confer plasticity to adhesive junctions. Recent studies indicate that the juxtamembrane domain of classical cadherins contains multiple endocytic motifs, or “switches”, that can be used by cellular membrane trafficking machinery to regulate adhesion. This review focuses on p120-catenin and other cadherin binding proteins, ubiquitin ligases, and growth factors as key modulators of cadherin membrane trafficking.

  5. Cresyl violet: a superior fluorescent lysosomal marker

    Philip P. Ostrowski, Gregory D. Fairn, Sergio Grinstein and Danielle E. Johnson

    Accepted manuscript online: 13 SEP 2016 04:20AM EST | DOI: 10.1111/tra.12447

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    We have identified cresyl violet as a superior fluorescent lysosomal marker. This red-shifted fluorophore accumulates selectively in lysosomes in a pH-dependent manner. Furthermore, we find that cresyl violet is remarkably photostable and does not photoconvert to other fluorescent species, unlike other widely used lysosomal probes. Cresyl violet does not alter the lysosomal buffering capacity, membrane integrity or luminal pH, it is widely available, and costs ≈ 30,000 times less than competing probes.