© John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze
Online ISSN: 1600-0854
ORIGINAL ARTICLE: Phosphatidylinositol 3,5-Bisphosphate-Rich Membrane Domains in Endosomes and Lysosomes
ORIGINAL ARTICLE: The Sec1/Munc18 Protein Groove Plays a Conserved Role in Interaction with Sec9p/SNAP-25
ORIGINAL ARTICLE: Phosphorylation of αSNAP is Required for Secretory Organelle Biogenesis in Toxoplasma gondii
REVIEW: The Crossroads of Synaptic Growth Signaling, Membrane Traffic and Neurological Disease: Insights from Drosophila
Recently Published Articles
- Myosins, actin and autophagy
Antonina J. Kruppa, John Kendrick-Jones and Folma Buss
Accepted manuscript online: 5 MAY 2016 02:05AM EST | DOI: 10.1111/tra.12410
Autophagy is an essential degradation pathway that delivers cytosolic components to the lysosome. This pathway recycles macromolecules for energy production and clears the cytosol from damaged proteins, organelles and invading pathogens. In this review, we will discuss the importance of actin filament dynamics for autophagy progression and highlight the distinct requirement for three classes of myosins during different stages of the autophagy pathway.
- Tropomyosin-Mediated Regulation of Cytoplasmic Myosins
Dietmar J. Manstein and Daniel P. Mulvihill
Article first published online: 4 MAY 2016 | DOI: 10.1111/tra.12399
The metazoan actin-based cytoskeleton facilitates an assortment of diverse cellular functions. This is made possible by the members of the tropomyosin multigene family, which at discrete cellular locations form well-defined copolymers with unique functional properties. Here, we present a unifying theory in which the tropomyosin isoform associating with the actin defines the surface landscape of the copolymer to determine the identity and activity of myosin motors that move upon it.
- Mechanics and Activation of Unconventional Myosins
Christopher Batters and Claudia Veigel
Article first published online: 3 MAY 2016 | DOI: 10.1111/tra.12400
A model showing an idealized unconventional myosin summarizing some of the mechanical specializations that they possess including the ability to backfold and unfold in the presence of calcium; for calmodulin to bind, rebind and change conformation; to produce the working stroke in two phases; to be dimeric and processive; to sense strain and to alter the kinetics dependent on the external load. The motor domains are shown in blue, the calmodulin/light chains are in yellow.
- Lipid Droplets Form from Distinct Regions of the Cell in the Fission Yeast Schizosaccharomyces pombe
Alex Meyers, Zuania P. del Rio, Rachael A. Beaver, Ryan M. Morris, Taylor M. Weiskittel, Amany K. Alshibli, Jaana Mannik, Jennifer Morrell-Falvey and Paul Dalhaimer
Article first published online: 29 APR 2016 | DOI: 10.1111/tra.12394
The authors show that lipid droplets form from different regions of fission yeast Schizosaccharomyces pombe cells based on the dominant neutral lipid of the nascent droplet. Droplets that are enriched in sterol esters form at the tips of polarized cells, whereas droplets that are enriched in triacylglycerols (TAGs) form around the nucleus. Elimination of TAGs completely abolishes lipid droplets, instead vesicle-shaped BODIPY 493/503 structures are observed. Thus, TAG seems necessary for lipid droplet biogenesis in these yeast cells.