© John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze
Online ISSN: 1600-0854
ORIGINAL ARTICLE: Phosphatidylinositol 3,5-Bisphosphate-Rich Membrane Domains in Endosomes and Lysosomes
ORIGINAL ARTICLE: The Sec1/Munc18 Protein Groove Plays a Conserved Role in Interaction with Sec9p/SNAP-25
ORIGINAL ARTICLE: Phosphorylation of αSNAP is Required for Secretory Organelle Biogenesis in Toxoplasma gondii
REVIEW: The Crossroads of Synaptic Growth Signaling, Membrane Traffic and Neurological Disease: Insights from Drosophila
Recently Published Articles
- Distinct roles of the two VPS33 proteins in the endolysosomal system in Caenorhabditis elegans
Keiko Gengyo-Ando, Eriko Kage-Nakadai, Sawako Yoshina, Muneyoshi Ohtori, Yuko Kagawa-Nagamura, Junichi Nakai and Shohei Mitani
Accepted manuscript online: 25 AUG 2016 03:46AM EST | DOI: 10.1111/tra.12430
Metazoan cells have two Vps33 proteins, VPS33A and VPS33B, which are thought to participate in endocytic membrane trafficking, but their precise roles remain unknown. Here, we used null mutant Caenorhabditis elegans to demonstrate that VPS-33.1 (VPS33A) is essential for viability and both early and late fusion events in the endocytic pathway, whereas VPS-33.2 (VPS33B) is not essential for these events but is required for spermatogenesis. Thus, animals might use these two proteins in distinct contexts.
- Vesicles Are Persistent Features of Different Plastids
Emelie Lindquist, Katalin Solymosi and Henrik Aronsson
Version of Record online: 24 AUG 2016 | DOI: 10.1111/tra.12427
Vesicles in plastids have been observed repeatedly, suggested to function in thylakoid biogenesis. Previous observations have mainly concerned proplastids and chloroplasts, often being pretreated to induce formation or inhibit fusion of vesicles. Here, we present vesicle-like structures in etioplasts, etio-chloroplasts, leucoplasts, chromoplasts and desiccoplasts, in addition to both proplastids and chloroplasts. They are here shown without any pretreatment of plants aiming to enhance vesicle appearance, and in different species (including both C3 and C4 plants), cell types and organs.
- Rab24 interacts with the Rab7/RILP complex to regulate endosomal degradation
Celina Amaya, Rodrigo D. Militello, Sebastián D. Calligaris and María I. Colombo
Accepted manuscript online: 23 AUG 2016 02:55AM EST | DOI: 10.1111/tra.12431
The Rab GTPases family coordinates the endocytic pathway. We have found Rab24 as a component of the endosome-lysosome degradative route. We demonstrate that Rab24 forms a complex with Rab7 and RILP on the membranes of LE, and its activity is essential for Rab7 membrane recruitment and DQ-BSA degradation. Additionally, overexpression of the HOPS subunit Vps41 hampered the Rab24/RILP association. Our data support that Rab24 owns a molecular function in the last steps of the endosomal degradative pathway via its interaction with critical components of the pathway.
- Identification of New Fungal Peroxisomal Matrix Proteins and Revision of the PTS1 Consensus
Christopher Nötzel, Thomas Lingner, Heiner Klingenberg and Sven Thoms
Version of Record online: 22 AUG 2016 | DOI: 10.1111/tra.12426
The peroxisomal targeting signal type 1 (PTS1) is the prevalent PTS. Its current definition, however, is largely incomplete. We have modelled PTS1 by a machine learning approach in yeast. Based on this, we identified two conserved genes encoding novel peroxisomal proteins and we updated the consensus motif to now include all PTS1 proteins in yeast.
- Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis
Sarah R. Elkin, Nathaniel W. Oswald, Dana K. Reed, Marcel Mettlen, John B. MacMillan and Sandra L. Schmid
Version of Record online: 8 AUG 2016 | DOI: 10.1111/tra.12425
The authors have characterized the natural product, ikarugamycin, to demonstrate its utility as a potent and selective acute inhibitor of clathrin-mediated endocytosis.