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Cover image for Vol. 18 Issue 10

Edited By: Michael S. Marks, Trina A. Schroer, Robert G. Parton and Sharon A. Tooze

Online ISSN: 1600-0854

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Advances in Cell Biology in China

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Celebrating the Contributions of Ari Helenius

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Chemical and Immunological Synapses"

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Recently Published Articles

  1. Phosphatidylinositol (4,5)-bisphosphate targets DOC2B to the plasma membrane

    Lirin Michaeli, Irit Gottfried, Maria Bykhovskaia and Uri Ashery

    Accepted manuscript online: 22 SEP 2017 09:55AM EST | DOI: 10.1111/tra.12528

    Thumbnail image of graphical abstract

    Synopsis

    DOC2B, a Ca2+ sensor, translocates from the cytosol to the plasma membrane (PM) upon calcium elevation and affects vesicle fusion. We show that DOC2B translocation is diffusion-driven and targeted to the PM by specific interactions with PI(4,5)P2, which anchors the protein to the membrane. Molecular dynamics simulations and mutagenesis revealed multiple PI(4,5)P2–DOC2B interactions and suggest that both calcium and PI(4,5)P2 binding are needed for DOC2B translocation. This demonstrates how membrane composition controls vesicle fusion and neuronal communication.

  2. Escherichia coliK1 utilizes host macropinocytic pathways for invasion of brain microvascular endothelial cells

    Lip Nam Loh, Elizabeth M. C. McCarthy, Priyanka Narang, Naveed A. Khan and Theresa H. Ward

    Version of Record online: 20 SEP 2017 | DOI: 10.1111/tra.12508

    Thumbnail image of graphical abstract

    Several clathrin-independent endocytic pathway components have been implicated as regulatory factors exploited by neonatal meningitis-causing Escherichia coliK1 bacteria to infect host cells. Here, we demonstrate that the bacteria induce general cell ruffling, increased fluid phase uptake, and actin-, Rho GTPase- and cholesterol-dependent invasion, which all point to a requirement for macropinocytosis as the route of uptake by the bacteria into non-phagocytic brain microvascular endothelial cells.

  3. MGRN1 mediated ubiquitination of α-tubulin regulates microtubule dynamics and intracellular transport

    Rukmini Mukherjee, Priyanka Majumder and Oishee Chakrabarti

    Accepted manuscript online: 13 SEP 2017 08:13AM EST | DOI: 10.1111/tra.12527

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    Microtubule-based transport is vital in neurons. Here we show that ubiquitination of α-tubulin by MGRN1 regulates microtubule polymer stability and efficiency of cargo transport on microtubule tracks. Functional loss of MGRN1 (by expression of catalytically inactive MGRN1 or presence of cytosolically exposed prion protein, CtmPrP) drastically compromises intracellular transport of mitochondria and endosomes. Hence, ubiquitination mediated microtubule dynamics is important in neurodegeneration associated with certain types of prion diseases.

  4. The dense-core vesicle maturation protein CCCP-1 binds RAB-2 and membranes through its C-terminal domain

    Jérôme Cattin-Ortolá, Irini Topalidou, Annie Dosey, Alexey J. Merz and Michael Ailion

    Version of Record online: 13 SEP 2017 | DOI: 10.1111/tra.12507

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    CCCP-1 is a coiled-coil protein important for dense-core vesicle (DCV) biogenesis. A structure-function analysis of CCCP-1 shows that its C-terminal domain is required for: (1) localization to membrane compartments near the trans-Golgi, (2) binding to activated RAB-2(3) function in DCV biogenesis and (4) direct binding to membranes. CCCP-1 has an elongated shape and forms oligomers. These findings suggest that CCCP-1 resembles members of the golgin family of proteins that act as membrane tethers.

  5. Fusion and scission of membranes: Ubiquitous topological transformations in cells

    Roland L. Knorr, Noboru Mizushima and Rumiana Dimova

    Version of Record online: 13 SEP 2017 | DOI: 10.1111/tra.12509

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    Remodeling of lipid membranes involves gradual bending as well as abrupt events: membrane fusion and membrane scission. Such discontinuous changes alter the number of individual (separate) membranes or the number of (torus-like) holes within the membrane structure: the topology of the structure changes. Here, we review cell membrane remodeling from a topological viewpoint, highlight the large number of topological changes during autophagy and link the two 2016 Nobel prizes honoring autophagy and topology.

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