Alphavirus restriction by IFITM proteins
Stuart Weston, Stephanie Czieso, Ian J. White, Sarah E. Smith, Rachael S. Wash, Carmen Diaz-Soria, Paul Kellam and Mark Marsh
Accepted manuscript online: 24 MAY 2016 02:35AM EST | DOI: 10.1111/tra.12416
IFITM3 can inhibit cellular infection by two alphaviruses, Sindbis and Semliki Forest virus (SFV). IFITM2 has lower antiviral activity, and IFITM1 does not restrict either virus through the endocytic route of infection. IFITM3 does not inhibit SFV binding, internalisation into cells, trafficking to endosomes or low pH-induced conformational changes in the E1 fusion protein. However, the viral capsid is not released into the cytosol in IFITM3 expressing cells.