Cover image for Vol. 16 Issue 2

Accepted Articles (Accepted, unedited articles published online and citable. The final edited and typeset version of record will appear in future.)

Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens, Sharon Tooze

Online ISSN: 1600-0854


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  1. Research Articles

    1. Molecular analysis and localization of CaARA7 a conventional RAB5 GTPase from characean algae

      Marion C. Hoepflinger, Anja Geretschlaeger, Aniela Sommer, Margit Hoeftberger, Christina Hametner, Takashi Ueda and Ilse Foissner

      Accepted manuscript online: 30 JAN 2015 05:02AM EST | DOI: 10.1111/tra.12267

      This is the first detailed investigation of a conventional RAB5 GTPase (CaARA7/CaRABF2) in a green alga closely related to land plants (Chara australis; Charales). CaARA7 is highly similar to other RAB5 proteins and has intrinsic GTPase activity. It is involved in endosomal membrane trafficking as suggested by localization experiments (immunolabelling of internodal cells using anti-CaARA7 as well as transient expression of different fluorescently tagged RAB5s in tobacco) and by protein mutations in membrane anchoring and GTP binding sites.

  2. Reviews

    1. Molecular control of B cell activation and immunological synapse formation

      Elina Kuokkanen, Vid Šuštar and Pieta K Mattila

      Accepted manuscript online: 30 JAN 2015 01:46AM EST | DOI: 10.1111/tra.12257

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    2. cphMolecular Underpinnings of Synaptic Vesicle Pool Heterogeneity

      Devon C. Crawford and Ege T. Kavalali

      Accepted manuscript online: 23 JAN 2015 10:51PM EST | DOI: 10.1111/tra.12262

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      In this review, Crawford and Kavalali discuss the various molecular components required to traffic synaptic vesicles to the presynaptic plasma membrane for synchronous evoked, asynchronous evoked, or spontaneous fusion. It is becoming clear that synaptic vesicles exhibit distinct molecular identities and a variety of molecules interact with vesicles to modulate their exocytosis in relation to neuronal action potentials. These distinct forms of neurotransmission, in turn, are critical for proper information transfer and synaptic signaling homeostasis throughout the nervous system.

  3. Research Articles

    1. cNew export pathway in Plasmodium falciparum-infected erythrocytes: role of the parasite group II chaperonin, PfTRiC

      Alassane Mbengue, Emilie Vialla, Laurence Berry, Gamou Fall, Nicolas Audiger, Edith Demettre-Verceil, David Boteller and Catherine Braun-Breton

      Accepted manuscript online: 23 JAN 2015 07:51AM EST | DOI: 10.1111/tra.12266

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      The malaria parasite type II chaperonin PfTRiC is exported to the host erythrocyte cytoplasm and translocates unfolded parasite proteins from the parasitophorous vacuole to the membrane of Maurer's clefts. Since some of these exported proteins have the PEXEL export signature, the PfTRiC cargos likely use the PTEX translocon to pass through the parasitophorous vacole membrane. Detection of PfTRiC in the parasitophorous vacuole lumen suggests a role for this chaperonin in the export of parasite proteins through different compartments.

    2. Disease-associated mutations of TREM2 alter the processing of N-linked oligosaccharides in the Golgi apparatus

      Ji-Seon Park, In Jung Ji, Hyun Joo An, Min-Ji Kang, Sang-wook Kang, Dong-Hou Kim and Seung-Yong Yoon

      Accepted manuscript online: 23 JAN 2015 07:51AM EST | DOI: 10.1111/tra.12264

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      Model for trafficking and glycosylation of wild type and mutant TREM2. Wild type TREM2 is normally trafficked and glycosylated from ER to Golgi and plasma membrane. However, Y38C or T66M mutant TREM2 is impaired in this process and accumulated in the ER to induce ER stress. R47H TREM2 is glycosylated differently from the wild type in the Golgi.

    3. Trafficking of acetyl-C16-ceramide-NBD with long-term stability and no cytotoxicity into the Golgi

      Tomohiko Makiyama, Hiroyuki Nakamura, Nobuo Nagasaka, Hisahiro Yamashita, Takuya Honda, Naoto Yamaguchi, Atsushi Nishida and Toshihiko Murayama

      Accepted manuscript online: 23 JAN 2015 07:51AM EST | DOI: 10.1111/tra.12265

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      A549 cells were labeled with a new ceramide having an acetylated C-1 hydroxyl group, C16-N-acyl chain, and 4-nitrobenzo-2-oxa-1,3-diazole-bound C15-sphingosine (acetyl-C16-ceramide-NBD), and the washed cells were further cultured for 36 hr. The left and right panels show an overall picture and a typical picture of daughter cells, respectively. Acetyl-C16-ceramide-NBD preferentially accumulated in the Golgi complex before and after mitosis. Because of its weaker cytotoxicity and resistance to ceramide metabolic enzymes, acetyl-C16-ceramide-NBD is useful as a Golgi-labeling probe in various cell types.

    4. Granule mobility, fusion frequency and insulin secretion are differentially affected by insulinotropic stimuli

      Kirstin Schumacher, Magnus Matz, Dennis Brüning, Knut Baumann and Ingo Rustenbeck

      Accepted manuscript online: 23 JAN 2015 07:31AM EST | DOI: 10.1111/tra.12261

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      The arrival of insulin granules at and departure from the plasma membrane is increased by glucose and high K+, accelerating the turnover. The number of granules remaining at the membrane decreases exponentially with time. The mobility pattern suggests that the majority of fusing granules is derived from long-term resident granules, but a highly mobile fast-fusing subgroup also exists. Increased cytosolic calcium affects fusion, but is not necessary for increased turnover. The actual secretion is additionally shaped by the heterogeneity of the post-fusion fate of the granules and (possibly) oscillatory regulation of fusion.

    5. Hepatitis E Virus enters liver cells through a Dynamin-2, Clathrin and membrane cholesterol dependent pathway

      R. Prasida Holla, Imran Ahmad, Zulfazal Ahmed and Shahid Jameel

      Accepted manuscript online: 23 JAN 2015 06:42AM EST | DOI: 10.1111/tra.12260

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      The hepatitis E virus (HEV) causes large outbreaks and sporadic cases of acute viral hepatitis in developing countries, and is associated with significant morbidity and mortality. Using bacterially expressed HEV-like particles and infectious virus, we have identified the determinants of HEV entry and intracellular trafficking in liver cells. This information is important for understanding HEV biology and may be useful in developing therapeutic interventions against hepatitis E.

    6. A second Las17 monomeric actin-binding motif functions in Arp2/3-dependent actin polymerization during endocytosis

      Daniel Feliciano, Thomas O. Tolsma, Kristen B. Farrell, Al Aradi and Santiago M. Di Pietro

      Accepted manuscript online: 23 JAN 2015 06:42AM EST | DOI: 10.1111/tra.12259

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      Actin polymerization driven by the Arp2/3 complex is essential for endocytosis. A second monomeric actin binding site, LGM, was mapped in Las17, the strongest activator of the Arp2/3 complex. LGM was characterized in vitro utilizing the yeast two-hybrid system, GST-pull down, fluorescence polarization, and pyrene-actin polymerization assays. The functional importance of LGM was established in vivo by analyzing the dynamics of actin polymerization, other components of the endocytic machinery and cargo internalization by live cell fluorescence microscopy.

  4. Reviews

    1. Organizing polarized delivery of exosomes at synapses

      Maria Mittelbrunn, Miguel Vicente Manzanares and Francisco Sánchez-Madrid

      Accepted manuscript online: 23 JAN 2015 06:40AM EST | DOI: 10.1111/tra.12258

      Exosome secretion is a finely tuned mechanism of intercellular communication. Cellular orientation and polarized fusion of multivesicular bodies are central events in this process to enable efficacy and specificity. We offer an integrated perspective of the roles of the different cellular cytoskeletons, small GTPases and phospholipid signaling circuits that drive the polarization of MVBs towards specific points of the plasma membrane and its function in crucial physiological processes including immune and neuronal synapses, cell migration and the function of epithelial sheets.

  5. Traffic Interchanges

    1. Tagging strategies strongly impact the fate of overexpressed caveolin-1

      Bing Han, Ajit Tiwari and Anne K. Kenworthy

      Accepted manuscript online: 30 DEC 2014 03:56AM EST | DOI: 10.1111/tra.12254

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      Our understanding of the properties and functions of caveolin-1 and caveolae is derived in part from transient overexpression studies utilizing C-terminally tagged caveolin-1 constructs. However, the impact of different tags on the behavior of overexpressed caveolin-1 has not been well studied. Here, we show here the ability of overexpressed caveolin-1 to oligomerize correctly and associate with detergent resistant membranes is heavily dependent on nature of the tag. Furthermore, endogenous caveolins are largely excluded from complexes containing tagged caveolin-1.

  6. Research Articles

    1. Atg27 tyrosine sorting motif is important for its trafficking and Atg9 localization

      Verónica A. Segarra, Douglas R. Boettner and Sandra K. Lemmon

      Accepted manuscript online: 30 DEC 2014 02:39AM EST | DOI: 10.1111/tra.12253

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      The transmembrane protein Atg27 facilitates transport of the major autophagy membrane protein, Atg9, to the pre-autophagosomal structure (PAS). This new study demonstrates that Atg27 has a tyrosine-sorting motif in its cytoplasmic tail that mediates its localization to the vacuole membrane by the AP-3 adaptor pathway. Moreover, this motif is also important for preventing Atg9 delivery to the vacuole lumen via the MVB pathway, suggesting that Atg27 retains Atg9 in endosomal reservoirs for mobilization during autophagy.

  7. Reviews

    1. Neuronal and Immune Synapses on the Move at Traffic

      Daniel D. Billadeau and Victor Faundez

      Accepted manuscript online: 18 DEC 2014 03:22PM EST | DOI: 10.1111/tra.12251


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