In vivo neuron-wide analysis of synaptic vesicle precursor trafficking
Celine I Maeder, Adriana San-Miguel, Emily Ye Wu, Hang Lu and Kang Shen
Accepted manuscript online: 9 DEC 2013 01:08PM EST | DOI: 10.1111/tra.12142
During synapse development, synaptic proteins must be targeted to presynaptic sites. Using neuron-wide time-lapse microscopy in C.elegans, we show that synaptic vesicle precursor (SVP) transport is differentially regulated in axon and dendrites and that polarized transport in concert with local vesicle capturing is necessary for accurate presynapse formation. SVP trafficking analysis of two hypomorphs for UNC-104/KIF-1A in combination with mathematical modelling identified movement directionality, entry of SVPs into the axon and axonal speeds as the important determinants of steady-state SVP distributions.