Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport
Gustaf E. Rydell, Henri-François Renard, Maria-Daniela Garcia-Castillo, Florent Dingli, Damarys Loew, Christophe Lamaze, Winfried Römer and Ludger Johannes
Accepted manuscript online: 4 APR 2014 08:57AM EST | DOI: 10.1111/tra.12173
SILAC labeling and quantitative mass spectrometry were used to identify Rab12 on Shiga toxin-induced endocytic plasma membrane invaginations. Localization of GFP-Rab12 on these structures was shown by fluorescence microscopy. Shiga toxin trafficking to trans-Golgi/TGN membranes and toxin-mediated inhibition of protein biosynthesis were inhibited in Rab12-depleted cells, suggesting that Rab12 functions in the retrograde transport route.