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Accepted Articles (Accepted, unedited articles published online and citable. The final edited and typeset version of record will appear in future.)

Edited By: Michael S. Marks, Trina A. Schroer, Professor R.G. Parton and Sharon A. Tooze

Online ISSN: 1600-0854

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  1. ORIGINAL ARTICLES

    1. Editorial process file includedExogenous Lysophospholipids with Large Head Groups Perturb Clathrin-Mediated Endocytosis

      Ieva Ailte, Anne Berit Dyve Lingelem, Audun Sverre Kvalvaag, Simona Kavaliauskiene, Andreas Brech, Gerbrand Koster, Paul Gunnar Dommersnes, Jonas Bergan, Tore Skotland and Kirsten Sandvig

      Accepted manuscript online: 9 JAN 2017 08:15AM EST | DOI: 10.1111/tra.12468

      Thumbnail image of graphical abstract

      Synopsis

      Exogenous addition of saturated lysophospholipids with large head groups to cells strongly inhibits clathrin-mediated endocytosis of transferrin: the number of endocytic pits decreases, the lifetime of AP2-positive pits increases and actin dependency is induced. Experiments with optical tweezers show that less force is required to pull membrane tubules outwards, suggesting that insertion of lysophospholipids in the outer leaflet bends the membrane outwards and counteracts formation of invaginations.

  2. TOOLBOX

    1. GFP-tagged Apolipoprotein E: a useful marker for the study of hepatic lipoprotein egress

      Constantin N. Takacs, Ursula Andreo, Rachel L. Belote, Joan Pulupa, Margaret A. Scull, Caroline E. Gleason, Charles M. Rice and Sanford M. Simon

      Accepted manuscript online: 30 DEC 2016 04:40AM EST | DOI: 10.1111/tra.12467

      Thumbnail image of graphical abstract

      Synopsis

      ApoE-GFP reproduces the behavior of untagged ApoE with respect to lipoprotein secretion from hepatic cells but does not support infectious hepatitis C virus production and is a useful marker for ApoE-containing hepatic lipoproteins.

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      DNA-based probes for flow cytometry analysis of endocytosis and recycling

      Claire Dumont, Ewa Czuba, Moore Chen, Jose A. Villadangos, Angus P.R. Johnston and Justine D. Mintern

      Accepted manuscript online: 26 DEC 2016 03:15AM EST | DOI: 10.1111/tra.12466

      Thumbnail image of graphical abstract

      Synopsis:

      We demonstrate the utility of a DNA-based specific hybridization internalization probe (SHIP) assay to examine endocytosis and recycling of ligands that bind to receptor proteins using flow cytometry. We monitored endocytosis of membrane-bound transferrin receptor and its soluble ligand transferrin in cell lines and primary cells. We demonstrate that internalization and recycling of holo-transferrin, and an antibody against the transferrin receptor behave differently. SHIP provides a convenient and high throughput technique for analysis of trafficking of cell surface receptors and ligands.

  3. Original Articles

    1. Amyloid precursor protein traffics from the Golgi directly to early endosomes in an Arl5b and AP4 dependent pathway

      Wei Hong Toh, Jing Zhi A. Tan, Khalisah L. Zulkefli, Fiona J. Houghton and Paul A. Gleeson

      Accepted manuscript online: 20 DEC 2016 11:58PM EST | DOI: 10.1111/tra.12465

      Thumbnail image of graphical abstract

      Synopsis

      The membrane trafficking of newly synthesised amyloid precursor protein (APP) from the Golgi to the endolysosomal system is not well defined, yet is critical for understanding the processing of APP. We demonstrate that the small G protein Arl5b recruits the adaptor complex AP4 to the trans-Golgi network (TGN) which regulates APP transport from the TGN directly to early endosomes. Our findings also show that the post-Golgi trafficking of newly synthesised APP is relevant for the regulation of amyloid β production.

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