Beyond attachment: roles of DC-SIGN in dengue virus infection
Ping Liu, Marc Ridilla, Pratik Patel, Laurie Betts, Emily Gallichotte, Lidea Shahidi, Nancy L. Thompson and Ken Jacobson
Accepted manuscript online: 27 JAN 2017 08:11AM EST | DOI: 10.1111/tra.12469
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Whether DC-SIGN functions as merely an attachment factor for dengue virus (DENV) or whether DC-SIGN plays further roles beyond attachment has been controversial. We use mammalian cell culture models, as well as primary dendritic cells, and high resolution, quantitative fluorescence microscopy to track the movements of DC-SIGN and DENV during viral entry. Our results support a model in which DC-SIGN captures DENV and participates, along with a co-receptor, in DENV internalization via clathrin-coated structures and subsequent trafficking to early endosomes.