Cover image for Vol. 17 Issue 7

Accepted Articles (Accepted, unedited articles published online and citable. The final edited and typeset version of record will appear in future.)

Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze

Online ISSN: 1600-0854


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  1. Reviews

    1. Rab35 GTPase: a central regulator of phosphoinositides and F-actin in endocytic recycling and beyond

      Kerstin Klinkert and Arnaud Echard

      Accepted manuscript online: 21 JUN 2016 10:30PM EST | DOI: 10.1111/tra.12422

      Thumbnail image of graphical abstract

      In the past ten years, Rab35 has become one of the most studied Rabs involved in a growing number of key cellular functions, including endosomal trafficking, exosome release, phagocytosis, cell migration, immunological synapse formation and neurite outgrowth. In addition, oncogenic activating mutations have been recently found in cancer patients. Here, we provide a comprehensive review on how Rab35 controls these apparently unrelated functions by regulating phosphoinositides and F-actin, both on endosomes and at the plasma membrane.

  2. Original Articles

    1. BMP2 Transfer to Neighboring Cells and Activation of Signaling

      Hamed Alborzinia, Marjan Shaikhkarami, Peter Hortschansky and Stefan Wölfl

      Accepted manuscript online: 16 JUN 2016 02:05AM EST | DOI: 10.1111/tra.12420

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      BMP signaling plays a central role in development and organ homeostasis, and is tightly regulated at various levels. Internalization of BMP2 by cells can provide another layer of regulation. We show that internalized BMP2 is transferred to neighboring cells and can induce BMP signaling. Cell-cell contact increased transfer and enhanced signaling. Noggin blocked signaling but also enhanced transfer. Inhibition of vesicular transport blocked transfer, without clear interference with signaling, suggesting that BMP2 signaling occurs independent of internalization and transfer.

    2. The HOPS/Class C Vps complex tethers high-curvature membranes via a direct protein-membrane interaction

      Ruoya Ho and Christopher Stroupe

      Accepted manuscript online: 16 JUN 2016 02:05AM EST | DOI: 10.1111/tra.12421

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      Here, we show that the HOPS membrane tethering complex can tether highly-curved membranes by binding to these membranes via the ALPS (amphipathic lipid packing sensor) motif in its Vps41p subunit. We propose that this protein-membrane interaction directs the localization of HOPS to the highly-curved “vertex ring” at the edge of the flattened zone of contact between tethered yeast vacuoles.

    3. Rab11 Regulates the Mast Cell Exocytic Response

      Joshua D. Wilson, Sarah A. Shelby, David Holowka and Barbara Baird

      Accepted manuscript online: 11 JUN 2016 01:15AM EST | DOI: 10.1111/tra.12418

      Mast cell exocytosis causes allergy through the release of mediators from secretory lysosomes; co-stimulated exocytosis of recycling endosomes provides a source of additional membrane and may play other roles. Here, we show that a dominant negative form of the recycling endosome protein Rab11 (S25N) interferes with both of these exocytic processes. Inhibition mediated by S25N Rab11 can be bypassed using compounds that block actin polymerization. Furthermore, inhibition of stimulated exocytosis by the F-actin stabilizer, jasplakinolide, is not additive with S25N Rab11, implicating Rab11 in actin remodeling necessary for exocytosis. Secretory lysosome (SL), recycling endosome (RE).

  3. Reviews

    1. Complex polarity: building multicellular tissues through apical membrane traffic

      Alvaro Roman-Fernandez and David M. Bryant

      Accepted manuscript online: 9 JUN 2016 08:56AM EST | DOI: 10.1111/tra.12417

      Thumbnail image of graphical abstract

      Cell polarization involves the asymmetric organization of the cell surface, which is often preceded by intracellular organelle rearrangements. We provide a focused survey of our current understanding of the interplay between the evolutionarily conserved polarity proteins that induce cell polarization, and the membrane trafficking machinery that facilitate such processes. We discuss the crucial roles this plays in allowing polarized cells and tissues to form.


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