Editorial process file includedExogenous Lysophospholipids with Large Head Groups Perturb Clathrin-Mediated Endocytosis
Ieva Ailte, Anne Berit Dyve Lingelem, Audun Sverre Kvalvaag, Simona Kavaliauskiene, Andreas Brech, Gerbrand Koster, Paul Gunnar Dommersnes, Jonas Bergan, Tore Skotland and Kirsten Sandvig
Accepted manuscript online: 9 JAN 2017 08:15AM EST | DOI: 10.1111/tra.12468
Exogenous addition of saturated lysophospholipids with large head groups to cells strongly inhibits clathrin-mediated endocytosis of transferrin: the number of endocytic pits decreases, the lifetime of AP2-positive pits increases and actin dependency is induced. Experiments with optical tweezers show that less force is required to pull membrane tubules outwards, suggesting that insertion of lysophospholipids in the outer leaflet bends the membrane outwards and counteracts formation of invaginations.