Lipid Droplets Form from Distinct Regions of the Cell in the Fission Yeast Schizosaccharomyces pombe
Alex Meyers, Zuania P. del Rio, Rachael A. Beaver, Ryan M. Morris, Taylor M. Weiskittel, Amany K. Alshibli, Jaana Mannik, Jennifer Morrell-Falvey and Paul Dalhaimer
Article first published online: 29 APR 2016 | DOI: 10.1111/tra.12394
The authors show that lipid droplets form from different regions of fission yeast Schizosaccharomyces pombe cells based on the dominant neutral lipid of the nascent droplet. Droplets that are enriched in sterol esters form at the tips of polarized cells, whereas droplets that are enriched in triacylglycerols (TAGs) form around the nucleus. Elimination of TAGs completely abolishes lipid droplets, instead vesicle-shaped BODIPY 493/503 structures are observed. Thus, TAG seems necessary for lipid droplet biogenesis in these yeast cells.