Cover image for Vol. 17 Issue 10

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze

Online ISSN: 1600-0854


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  1. Toolbox

    1. Cresyl violet: a superior fluorescent lysosomal marker

      Philip P. Ostrowski, Gregory D. Fairn, Sergio Grinstein and Danielle E. Johnson

      Version of Record online: 17 OCT 2016 | DOI: 10.1111/tra.12447

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      We have identified cresyl violet as a superior fluorescent lysosomal marker. This red-shifted fluorophore accumulates selectively in lysosomes in a pH-dependent manner. Furthermore, we find that cresyl violet is remarkably photostable and does not photoconvert to other fluorescent species, unlike other widely used lysosomal probes. Cresyl violet does not alter the lysosomal buffering capacity, membrane integrity or luminal pH, it is widely available, and costs ≈30 000 times less than competing probes.

  2. Traffic Interchange

    1. An antimicrobial origin of transit peptides accounts for early endosymbiotic events

      Francis-André Wollman

      Version of Record online: 12 OCT 2016 | DOI: 10.1111/tra.12446

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      Primary endosymbiosis, which gave rise to mitochondria or chloroplasts, required successful targeting of a number of proteins from the host cytosol to the endosymbiotic organelles. A survey of studies published in separate fields of biological research over the past 40 years argue for an antimicrobial origin of targeting peptides. It is proposed that mitochondria and chloroplast derive from microbes that developed a resistance strategy to antimicrobial peptides that consisted in their rapid internalization and proteolytic disposal by microbial peptidases.

  3. Toolbox

    1. You have full text access to this OnlineOpen article
      Using HHsearch to tackle proteins of unknown function: A pilot study with PH domains

      David R. Fidler, Sarah E. Murphy, Katherine Courtis, Pantelis Antonoudiou, Rana El-Tohamy, Jonathan Ient and Timothy P. Levine

      Version of Record online: 9 OCT 2016 | DOI: 10.1111/tra.12432

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      HHsearch (available online as HHpred) is a powerful bioinformatics tool that examines the predicted 3D structure of a protein to detect remote protein homologies. We have applied HHsearch to study a single structural fold (PH and PH-like domains) in a single model organism (yeast) as proof of principle. HHsearch detected known domains in yeast with 99% specificity as well 16 new PH-like domains in 13 proteins. We corroborated the functional importance of 1 predicted domain in Vps13. Similar remote homology searches should be applied genome-wide.

  4. Original Articles

    1. You have full text access to this OnlineOpen article
      KIF1A mediates axonal transport of BACE1 and identification of independently moving cargoes in living SCG neurons

      Christy O. Y. Hung and Michael P. Coleman

      Version of Record online: 5 OCT 2016 | DOI: 10.1111/tra.12428

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      Using two-colour live-cell imaging, we found that KIF1A is the primary anterograde motor protein mediating the axonal transport of beta-secretase 1 (BACE1). We further identified several cargoes that have little or no co-migration with KIF1A-GFP and also move independently from BACE1-mCherry. Our findings suggest that axonally transported cargoes are sorted into different classes of carrier vesicles in the cell body and are transported by cargo-specific motor proteins through the axon.

    2. Rab24 interacts with the Rab7/Rab interacting lysosomal protein complex to regulate endosomal degradation

      Celina Amaya, Rodrigo D. Militello, Sebastián D. Calligaris and María I. Colombo

      Version of Record online: 3 OCT 2016 | DOI: 10.1111/tra.12431

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      The Rab GTPases family coordinates the endocytic pathway. We have found Rab24 as a component of the endosome-lysosome degradative route. We demonstrate that Rab24 forms a complex with Rab7 and RILP on the membranes of LE, and its activity is essential for Rab7 membrane recruitment and DQ-BSA degradation. Additionally, overexpression of the HOPS subunit Vps41 hampered the Rab24/RILP association. Our data support that Rab24 owns a molecular function in the last steps of the endosomal degradative pathway via its interaction with critical components of the pathway.

    3. Distinct roles of the two VPS33 proteins in the endolysosomal system in Caenorhabditis elegans

      Keiko Gengyo-Ando, Eriko Kage-Nakadai, Sawako Yoshina, Muneyoshi Otori, Yuko Kagawa-Nagamura, Junichi Nakai and Shohei Mitani

      Version of Record online: 3 OCT 2016 | DOI: 10.1111/tra.12430

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      Metazoan cells have two Vps33 proteins, VPS33A and VPS33B, which are thought to participate in endocytic membrane trafficking, but their precise roles remain unknown. Here, we used null mutant Caenorhabditis elegans to demonstrate that VPS-33.1 (VPS33A) is essential for viability and both early and late fusion events in the endocytic pathway, whereas VPS-33.2 (VPS33B) is not essential for these events but is required for spermatogenesis. Thus, animals might use these two proteins in distinct contexts.

    4. Tracking adenovirus genomes identifies morphologically distinct late DNA replication compartments

      Tetsuro Komatsu, Derrick R. Robinson, Miharu Hisaoka, Shuhei Ueshima, Mitsuru Okuwaki, Kyosuke Nagata and Harald Wodrich

      Version of Record online: 3 OCT 2016 | DOI: 10.1111/tra.12429

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      Synopsis The spatial relationship between early adenoviral genome replication and subsequent viral gene expression has been established. In contrast, when and where newly replicated genomes are linked to progeny virion production remains unclear. Using direct genome-labeling techniques, we show the formation of a novel subnuclear compartment late in infection, which we named Virus-induced Post-Replication (ViPR) body that functions as a reservoir for replicated adenoviral genomes, and identified a host factor specifically localizing in the domain. The potential role of ViPR bodies is discussed.

  5. Reviews

    1. Complex Polarity: Building Multicellular Tissues Through Apical Membrane Traffic

      Alvaro Román-Fernández and David M. Bryant

      Version of Record online: 1 JUL 2016 | DOI: 10.1111/tra.12417

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      Cell polarization involves the asymmetric organization of the cell surface, which is often preceded by intracellular organelle rearrangements. We provide a focused survey of our current understanding of the interplay between the evolutionarily conserved polarity proteins that induce cell polarization and the membrane trafficking machinery that facilitate such processes. We discuss the crucial roles of these proteins in the formation of polarized cells and tissues.

    2. Cadherin Trafficking for Tissue Morphogenesis: Control and Consequences

      Junior J. West and Tony J. C. Harris

      Version of Record online: 19 MAY 2016 | DOI: 10.1111/tra.12407

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      Tissue morphogenesis often requires the redistribution of cadherin cell–cell adhesion molecules through trafficking. We review the upstream control and downstream consequences of this trafficking in a variety of model systems. We describe how core trafficking machinery integrates with other molecular systems to control cadherin endocytosis, exocytosis and recycling, and how this trafficking has permissive and instructive effects on various aspects of multicellular development.


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