Cover image for Vol. 18 Issue 1

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Michael S. Marks, Trina A. Schroer, Robert G. Parton and Sharon A. Tooze

Online ISSN: 1600-0854


  1. 1 - 6

    1. Fundamental mechanisms deliver the Nobel Prize to Ohsumi

      Sharon A. Tooze, Lisa A. Hannan, Michael S. Marks, Tom H. Stevens and Trina A. Schroer

      Version of Record online: 17 JAN 2017 | DOI: 10.1111/tra.12460

  2. Original Articles

    1. Targeting of tail-anchored membrane proteins to subcellular organelles in Toxoplasma gondii

      Leah R. Padgett, Gustavo Arrizabalaga and William J. Sullivan Jr.

      Version of Record online: 17 JAN 2017 | DOI: 10.1111/tra.12464

      Thumbnail image of graphical abstract

      Subcellular localization of tail-anchored (TA) proteins is dependent on targeting sequences within the C-terminus. We performed the first exploration of TA proteins in the phylum Apicomplexa using the protozoan parasite Toxoplasma gondii as a model. We identified 59 putative TA proteins in the Toxoplasma genome and examined the organellar targeting mechanisms for 9 representatives. These findings provide novel insight into this uncharacterized class of proteins in protozoan parasites and how they traffic to different subcellular compartments.


    1. Mitochondrial protein import in trypanosomes: Expect the unexpected

      Anke Harsman and André Schneider

      Version of Record online: 17 JAN 2017 | DOI: 10.1111/tra.12463

      Thumbnail image of graphical abstract

      A comparative analysis of mitochondrial protein import in yeast and trypanosomes, which are only remotely related, reveals an amazing diversity in the composition of the respective machineries. This is surprising since the protein import systems essentially have the same function in all mitochondria. The differences between the import systems provide exciting examples of convergent evolution over large phylogenetic distances.

  4. Original Articles

    1. Distinct complexes of yeast Snx4 family SNX-BARs mediate retrograde trafficking of Snc1 and Atg27

      Mengxiao Ma, Christopher G. Burd and Richard J. Chi

      Version of Record online: 16 JAN 2017 | DOI: 10.1111/tra.12462

      Thumbnail image of graphical abstract

      Live cell imaging of individual yeast endosomes shows that the Snx4 and the Vps5-Vps17 retromer sorting nexins operate concurrently on a maturing endosome. The 3 Snx4 family proteins form 2 functionally distinct heterodimers: Snx4-Atg20 and Snx4-Snx41. Each heterodimer coats an endosome-derived tubule that mediates retrograde sorting of distinct cargo; the v-SNARE, Snc1, is a cargo of the Snx4-Atg20 pathway, and Snx4-Snx41 mediates retrograde sorting of Atg27, an integral membrane protein implicated in selective autophagy.


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      The C-terminal tails of heterotrimeric kinesin-2 motor subunits directly bind to α-tubulin1: Possible implications for cilia-specific tubulin entry

      Mukul Girotra, Shalini Srivastava, Anuttama Kulkarni, Ayan Barbora, Kratika Bobra, Debnath Ghosal, Pavithra Devan, Amol Aher, Akanksha Jain, Dulal Panda and Krishanu Ray

      Version of Record online: 5 JAN 2017 | DOI: 10.1111/tra.12461

      Thumbnail image of graphical abstract

      Tubulin entry into the cilia and flagella is essential for their growth. The article by Girotra et al suggests that the tail domains of heterotrimeric kinesin-2 motor subunits can selectively bind certain α-tubulin1 isotypes and regulate the entry of tubulin into cilia. The conjecture is supported by extensive binding studies in vitro and tissue-cultured cells using recombinant tail fragments of both Drosophila and mouse orthologues, as well as genetic analysis in Drosophila.

    2. Cargo selectivity of yeast sorting nexins

      Björn D.M. Bean, Michael Davey and Elizabeth Conibear

      Version of Record online: 3 JAN 2017 | DOI: 10.1111/tra.12459

      Thumbnail image of graphical abstract

      Yeast sorting nexins direct membrane proteins on different endosomal trafficking pathways, but the number of known cargos is limited, and few sorting signals have been defined. Here, we used a large-scale imaging screen to find 23 new sorting nexin cargo proteins, and identified cytosolic sequences that are necessary for sorting nexin-dependent localization and endosomal recycling. This allowed us to define a consensus sequence for Snx3 and retromer-dependent cargo sorting.


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