Host PI(3,5)P2 Activity Is Required for Plasmodium berghei Growth During Liver Stage Infection
Carolina Thieleke-Matos, Mafalda Lopes da Silva, Laura Cabrita-Santos, Cristiana F. Pires, José S. Ramalho, Ognian Ikonomov, Elsa Seixas, Assia Shisheva, Miguel C. Seabra and Duarte C. Barral
Article first published online: 20 AUG 2014 | DOI: 10.1111/tra.12190
The asymptomatic liver stage of malaria infection provides an opportunity for therapeutic intervention to block disease progression and transmission. However, the interaction of Plasmodium parasites with host liver cells remains largely obscure. We found that host PI(3,5)P2 phosphoinositide and its effector TRPML1 are necessary for Plasmodium growth. Hence, we propose that host endocytic vesicles fuse with the parasite vacuole, allowing the exchange of material between the host and the parasite, necessary for parasite replication in the liver.