Class III Phosphoinositide 3-Kinase/VPS34 and Dynamin are Critical for Apical Endocytic Recycling
Sarah Carpentier, Francisca N'Kuli, Giuseppina Grieco, Patrick Van Der Smissen, Virginie Janssens, Hervé Emonard, Benoît Bilanges, Bart Vanhaesebroeck, Héloïse P. Gaide Chevronnay, Christophe E. Pierreux, Donatienne Tyteca and Pierre J. Courtoy
Article first published online: 3 JUN 2013 | DOI: 10.1111/tra.12079
Recycling is a limiting step for receptor-mediated endocytosis. We here show that class III PI3K is the key isoform involved, based on differential pharmacological inhibition (substractive approach) and PI-3P sequestration by overexpressed GFP-(FYVE)x2 in cultured kidney epithelial cells, as well as conditional PI3K-III/Vps34 KO in developing mice kidneys. Acute reversal of PI3K inhibition induced a spectacular burst of recycling tubules that recruited dynamin-GFP and depended on its GTPase activity. This simple procedure may help further dissect recycling machineries.