Cover image for Vol. 17 Issue 12

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze

Online ISSN: 1600-0854


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  1. Original Articles

    1. 14-3-3 Proteins regulate K2P5.1 surface expression on T lymphocytes

      Juncal Fernández-Orth, Petra Ehling, Tobias Ruck, Susann Pankratz, Majella-Sophie Hofmann, Peter Landgraf, Daniela C. Dieterich, Karl-Heinz Smalla, Thilo Kähne, Guiscard Seebohm, Thomas Budde, Heinz Wiendl, Stefan Bittner and Sven G. Meuth

      Version of Record online: 27 NOV 2016 | DOI: 10.1111/tra.12455

      Thumbnail image of graphical abstract

      K2P5.1 channels possess a putative non-classical consensus motif for 14-3-3 proteins that mediates the interaction and promotes K2P5.1 channels to the plasma membrane. An amino acid mutation reduces the binding of 14-3-3 proteins to K2P5.1 resulting in a reduced number of channels at the plasma membrane and a decreased potassium efflux. Pharmacological inhibition of 14-3-3 protein binding to K2P5.1 functionally impacts T-cell proliferation and cytokine production. 14-3-3 proteins may represent a pharmacological target for the treatment of multiple sclerosis and other autoimmune diseases.

  2. Reviews

    1. Microbe-inducible trafficking pathways that control Toll-like receptor signaling

      Yunhao Tan and Jonathan C. Kagan

      Version of Record online: 23 NOV 2016 | DOI: 10.1111/tra.12454

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      In the context of lipopolysaccharide (LPS) stimulation, CD14 and MD-2 serve as Transporters Associated with the eXecution of Inflammation (TAXI) proteins for Toll-like receptors 4 (TLR4) to trigger plasma membrane myddosome formation and induce proinflammatory cytokine production. Subsequent endocytosis of TLR4 activates the TRAM and TRIF-dependent signaling process leading to type I IFN production. Intracellular TLR4 signaling could occur at distinct compartments including endosomes, multivesicular bodies (MVBs), phagosomes. Ubiquitinylation and hepatocyte growth factor regulated tyrosine kinase substrate (HRS)-mediated sorting processes have been implicated in the intracellular sorting of TLR4. In the context of lipotechoic acids (LTA) stimulation, MBL functions as a TAXI protein for the TLR2/6 heterodimer. MBL delivers LTA to the phagosome where a TLR2/6 heterodimer may promote myddosome formation to induce proinflammatory cytokine production.

  3. Toolbox

    1. Polyglutamine toxicity in yeast uncovers phenotypic variations between different fluorescent protein fusions

      Yuwei Jiang, Sonja E. Di Gregorio, Martin L. Duennwald and Patrick Lajoie

      Version of Record online: 1 NOV 2016 | DOI: 10.1111/tra.12453

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      Fluorescent proteins have revolutionized the work of scientists by allowing the direct visualization of protein trafficking and localization in living cells. However, fluorescent proteins are too often seen as interchangeable building blocks than can be easily swapped to accommodate a specific imaging condition. In this study, we used a yeast model of Huntington's diseases to highlight the differential impact of the most common fluorescent proteins on the aggregation and toxicity of expanded polyglutamine proteins.

  4. Reviews

    1. An evolutionary balance: conservation vs innovation in ciliate membrane trafficking

      Sabrice Guerrier, Helmut Plattner, Elisabeth Richardson, Joel B. Dacks and Aaron P. Turkewitz

      Version of Record online: 27 OCT 2016 | DOI: 10.1111/tra.12450

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      Several species of ciliates represent useful experiment organisms for cell biology research, and particularly for analyzing the outcomes of evolutionary trajectories that are distant from those of fungi or animals. This review focuses on 3 phenomena in ciliates that are all related to conserved pathways of endolysosomal trafficking, but with unique ciliate features: phagosome formation and maturation, regulated exocytosis of secretory granules, and programmed degradation of specific nuclei during conjugation.


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