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Mitsunori Fukuda

Article first published online: 10 JUN 2013 | DOI: 10.1111/tra.12083

Small GTPase Rab Rab27 is widely conserved in metazoan, and two Rab27 isoforms, Rab27A and Rab27B, are present in vertebrates. Although Rab27A and Rab27B were initially thought to function redundantly by sharing common Rab27 effectors in secretory pathways, recent evidence has indicated that by interacting with different Rab27 effectors they play different roles in special types of secretion. In this review article I describe the current state of our understating of the functions of Rab27 effectors in secretory pathways.

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Frank Adolf, Alexia Herrmann, Andrea Hellwig, Rainer Beck, Britta Brügger and Felix T. Wieland

Article first published online: 10 JUN 2013 | DOI: 10.1111/tra.12084

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Alexander G. Georgiev, Jesper Johansen, Vidhya D. Ramanathan, Yves Y. Sere, Christopher T. Beh and Anant K. Menon

Article first published online: 6 JUN 2013 | DOI: 10.1111/tra.12082

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Sarah Carpentier, Francisca N'Kuli, Giuseppina Grieco, Patrick Van Der Smissen, Virginie Janssens, Hervé Emonard, Benoît Bilanges, Bart Vanhaesebroeck, Héloïse P. Gaide Chevronnay, Christophe E. Pierreux, Donatienne Tyteca and Pierre J. Courtoy

Article first published online: 3 JUN 2013 | DOI: 10.1111/tra.12079

Recycling is a limiting step for receptor-mediated endocytosis. We here show that class III PI3K is the key isoform involved, based on differential pharmacological inhibition (substractive approach) and PI-3P sequestration by overexpressed GFP-(FYVE)x2 in cultured kidney epithelial cells, as well as conditional PI3K-III/Vps34 KO in developing mice kidneys. Acute reversal of PI3K inhibition induced a spectacular burst of recycling tubules that recruited dynamin-GFP and depended on its GTPase activity. This simple procedure may help further dissect recycling machineries.

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Karine Frénal, Chwen L. Tay, Christina Mueller, Ellen S. Bushell, Yonggen Jia, Arnault Graindorge, Oliver Billker, Julian C. Rayner and Dominique Soldati-Favre

Article first published online: 29 MAY 2013 | DOI: 10.1111/tra.12081

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Thomas Schmiedinger, Georg F. Vogel, Oliver Eiter, Kristian Pfaller, Walter A. Kaufmann, Angelika Flörl, Karin Gutleben, Sabine Schönherr, Barbara Witting, Thomas W. Lechleitner, Hannes-L. Ebner, Thomas Seppi and Michael W. Hess

Article first published online: 23 MAY 2013 | DOI: 10.1111/tra.12080

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Elsa Seixas, Mafalda Barros, Miguel C. Seabra and Duarte C. Barral

Article first published online: 29 APR 2013 | DOI: 10.1111/tra.12072

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