Traffic

Cover image for Vol. 18 Issue 1

Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze

Online ISSN: 1600-0854

Cover Gallery Volume 1


Volume 1

For covers from other volumes, go back to the Cover Gallery index.

Browse the covers of Volume 1 below.

Cover Image

Vol. 1, Iss. 12, Dec 2000

Cover illustration: Actin cytoskeleton reorganization through the cell cycle.
The yeast Saccharomyces cerevisiae actin cytoskeleton undergoes complex reorganization events during a cell cycle. Fluorescent rhodamine phalloidin bound to filamentous actin (orange) is shown superimposed to differential contrast micrographs (green). Four distinct actin structures are visible during the two hours reproduction cycle: caps, patches, cables and a bud-neck ring. (Image courtesy of D Ursic and MR Culbertson, University of Wisconsin).
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Cover Image

Vol. 1, Iss. 11, Nov 2000

Cover illustration: Altered trafficking of a fluorescent sphingolipid analog in lipid storage diseases.
Human skin fibroblasts were incubated with BODIPY-lactosylceramide at 37°C and observed under the fluorescence microscope. The fluorescence emission of the BODIPY-fluorophore changes from green to red wavelengths with increasing concentrations in membranes. During endocytosis, the lipid analog is targeted primarily to the Golgi apparatus in normal cells (inset), but accumulates in late endosomes and lysosomes in lipid storage disease fibroblasts, as shown here for Niemann Pick, Type C disease. (Pagano R et al.Traffic 2000; 1).
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Cover Image

Vol. 1, Iss. 10, Oct 2000

Cover illustration: This Swiss 3T3 fibroblast has been injected with a domain from p21-activated kinase that binds to activated Cdc42 and Rac. The domain is labeled with Alexa 546 dye (Molecular Probes). Warmer colors (i.e. red) correspond to higher probe concentrations, indicating regions of Cdc42 and/or Rac activation. Such labeled domains can reveal the distribution of native protein activation as it changes in real time within living cells. (See review Chamberlain and Hahn, Traffic 2000; 1).
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Cover Image

Vol. 1, Iss. 9, Sep 2000

Cover illustration: Brefeldin A effects on MDCK cells. MDCK cells expressing the apical early endosomal protein endotubin were incubated with brefeldin A for 10 minutes at 37°C, washed, and incubated for 2 minutes at 37°C. Cells were labeled to visualize endotubin (green) and internalized transferrin (red). Rapid separation of endotubin and transferrin is seen after this short BFA washout. Gokay and Wilson, Traffic 1(5):354.
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Cover Image

Vol. 1, Iss. 8, Aug 2000

Cover illustration: Structure of Rab GDI. The cover illustrates a ribbon diagram of the 1.04 Å structure of guanine nucleotide dissociation inhibitor (a?-GDI) (Peng et al. (2000), Traffic, 1(3) : 270-281), a protein that regulates the recycling of Rab GTPases involved in vesicle targeting and fusion in the exocytic and endocytic membrane trafficking pathways. The 'mobile effector loop' (fluorescent green) projecting from the protein is responsible for membrane-association of GDI. In the background is a cell expressing GFP-tagged Rab1 showing the distribution of Rab1 (green) and syntaxin 5 (an endogenous targeting/fusion factor) (red) and their co-localization (yellow) on pre-Golgi intermediates and Golgi compartments. The mobile effector loop of GDI is believed to mediate the docking of GDI onto these membranes to direct retrieval of Rab1 to the cytosol following vesicle fusion.
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Cover Image

Vol. 1, Iss. 7, Jul 2000

Cover illustration: Virus like particles in intracellular vesicles following expression of the simian immunodeficiency virus Gag precursor protein in Cos cells. Cells were electroporated to intoduce the SIV Gag orf cloned into pRK5 and cultured for two days. The cells were fixed, embedded in Epon and thin sections were viewed by transmission EM. The negatives were scanned to generate PICT files that were then pseudocolored using Adobe Photoshop. The Cos cell cytoplasm is blue, the gag particles dark red and the lumen of the vacuole beige. The particles have a mean diameter of 120 ± 10 nm. Submitted by Wolfgang Ballensiefen, Annegret Pelchen-Matthews, Mark Shipman and Mark Marsh Medical Research Council Laboratory for Molecular Cell Biology.
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Cover Image

Vol. 1, Iss. 6, Jun 2000

Cover illustration: Structure of the nuclear pore complex. The background represents an energy-filtered transmission electron microscopy image of a native, unstained and unfixed Xenopus nuclear envelope embedded in a thick ice layer (>200 nm). The foreground represents a novel 3-D reconstruction of the nuclear pore complex by zero-loss filtered electron tomography of the specimen depicted in the background. Image by D. Stoffler & U. Aebi.
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Cover Image

Vol. 1, Iss. 5, May 2000

Cover illustration: Crystallographic model of the 3 legs of a clathrin triskelion at 2.6A resolution from Joel Ybe and Peter Hwang, University of California, San Francisco, superimposed against an electron micrograph image of the clathrin lattice taken by Inke Nathke and John Heuser, Washington University, St. Louis. The model is based on the solved structure highlighted in white (Ybe et al (1999) Nature 399: 371-375), which contains two repeated structural motifs, of which seven are predicted to constitute the entire filamentous portion of the clathrin triskelion leg. The structural image was generated by the program Insight II (Molecular Simulations, Inc.).
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Cover Image

Vol. 1, Iss. 4, Apr 2000

Cover illustration: Immunofluorescence microscopy of mature human bone marrow derived dendritic cells (DC) stained for MHC class II molecules. Upon stimulation with inflammatory molecules such as (LPS or TNF-alpha), DCs adopt a typical dendritic morphology while the bulk of MHC class II molecules relocate to the cell surface and to the CIIV class II compartment. Picture courtesy of Evelina Gatti and Philippe Pierre (CIML, Marseille).
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Cover Image

Vol. 1, Iss. 3, Mar 2000

Cover illustration: A GPI-anchored protein, gD1-DAF expressed in Madin Darby Canine Kidney cells which have been deprived of of their exogenous cholesterol source, LDL for 3 days. There is an intracellular pool of gD1-DAF, but labeled gD1-DAF (red) does not colocalize with Di-0-C6 (green), a marker for the endoplasmic reticulum. (Hannan, LA and Edidin, M. J Cell Biol 1996;133:1265-1276.)
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Cover Image

Vol. 1, Iss. 2, Feb 2000

Cover illustration: Scanning electron micrograph of a Trypansoma cruzi trypomastigote invading a culture Madin-Darby canine kidney epithelial cell. The image is magnified 13900 fold. Courtesy of Edith S. Robbins, New York University School of Medicine, Department of Cell Biology, New York, NY 10016, USA.
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Cover Image

Vol. 1, Iss. 1, Jan 2000

Cover illustration: Cellular tracks and motors: Actin and microtubules with attached molecular motors. Shown are side and end on views of renderings of three-dimensional maps calculated from electron micrographs. (see Rayment et al. Science 261:58 - 65 and Sosa et al. 1997 Cell 90: 217 - 224). Images courtesy of Abel W. Lin, Mike Whittaker and Ron Milligan , The Scripps Research Institute.
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