© John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens, Sharon A. Tooze
Online ISSN: 1600-0854
Cover Gallery Volume 12
For covers from other volumes, go back to the Cover Gallery index.
Browse the covers of Volume 12 below.
Vol. 12, Iss. 12, December 2011
Cover Legend: The electron micrograph shows concentric stacks of organised smooth endoplasmic reticulum (ER) induced by expression of an envelope protein encoded by African Swine Fever virus.
ER cisternae are open in the outer and interior stacks of ER that face the cytosol. The inner stacks have collapsed ER cisternae. ER collapse is driven by disulphide bonds between N-terminal domains of the envelope protein arranged in antiparallel arrays across the ER lumen (see Windsor et al., Traffic 2011; 13(1).)
Vol. 12, Iss. 11, November 2011
Cover Legend: Scanning electron micrograph of pollen tubes growing through the pistil of an Arabidopsis thaliana flower and entering the ovules
Growing pollen tubes are able to rapidly change the orientation of their growth activity in order to respond to directional cues. This reorientation of growth is accomplished by reorienting the growth machinery, a dense aggregation of secretory vesicles that moves in precisely identified spatial patterns within the tubular cell (see Bou Daher and Geitmann, Traffic 2011; 12(11): 1537–1551).
Vol. 12, Iss. 10, October 2011
Cover Legend: The 14-3-3-like soluble domain of Mdm38
The 14-3-3-like soluble domain of Mdm38 is a mitochondrial inner membrane protein. The 14-3-3-like domain links mitochondrial ribosomes to the matrix side of the inner mitochondrial membrane (bottom) and is required for translation of a subset of mitochondria-encoded proteins (see Lupo et al., Traffic 2011; 12(10): 1457–1466).
Vol. 12, Iss. 9, September 2011
Cover Legend: Localization of ESCRT subunits in multivesicular endosomes
HEp2 cells expressing a GTPase-deficient Rab5 mutant that causes enlarged endosomes were fixed and stained with anti-EEA1 (green) and components of the four endosomal sorting complexes required for transport (ESCRTs). Upper left: ESCRT-0, upper right: ESCRT-I, lower left: ESCRT-II, lower right: ESCRT-III (see Malerød et al., Traffic 2011; 12(9):1211-1226).
Vol. 12, Iss. 8, August 2011
Cover Legend: Proposed functions of tethers and scaffolds in the organisation of the TGN
Functions include connecting the TGN with transport carriers emerging from endosomes, promoting the biogenesis of anterograde membrane carriers, facilitating microtubule interactions with the TGN, and as highlighted in this issue and circled, the tethering of retrograde transport carriers with the TGN prior to membrane fusion (see Chia and Gleeson, Traffic 2011; 12(8): 939-947).
Vol.12 Iss.7, July 2011
Cover Legend: High pressure frozen THP-1 monocytes were sectioned and chemically fixed according to the VIS2FIXH method
Subsequently, the sections were immuno-labeled with gold and fluorescent markers for LAMP2, located to lysosomes. The sections were imaged with integrated laser and electron microscopy (iLEM). Positively labeled cells are located based on fluorescence signal (green) and can be investigated with high resolution in the TEM (inset). In the inset, two lipid droplets are shown with electron density inside (left, in close proximity to positively labeled lysosomes, and right, with higher electron density). The VIS2FIXH method allows for the fixation and retention of the neutral lipids, found in the core of the lipid droplets (see Karreman et al., Traffic 2011; 12(7):806-814).
Vol.12 Iss.6, June 2011
Cover Legend: The facilitative glucose transporter GLUT4 translocates to the cell surface of adipocytes in response to insulin
3T3-L1 adipocytes expressing a version of the facilitative glucose transporter GLUT4 harbouring an HA-epitope tag in its first exofacial loop were either treated with 100nM insulin (right-hand panel) or not (left-hand panel) for 30 minutes prior to labelling for surface (blue) and total (green) GLUT4. Surface GLUT4 was labelled with HA antibody without cell permeabilisation. Total GLUT4 was labelled with rabbit-anti GLUT4 antibodies following permeabilisation with 0.1% saponin. Surface labelling was detected with Alexa-647 and total with Alexa-488 conjugated secondary antibodies. Optical sections were analyzed by confocal laser scanning mocroscopy using a Zeiss LSM PascalExciter fluorescence system. Fluorophores were scanned separately and images overlaid using LSM software. Image by Iain Adamson (Bryant lab) (see Bryant and Gould, Traffic 2011; 12(6): 657-664).
Vol.12 Iss.5, May 2011
Cover Legend: Uropathogenic Escherichia coli (UPEC) entry in endothelial cells
Immunofluorescence of HUVEC expressing YFP-Rac1L61 (green) infected with the J96 UPEC strain. Bacteria were labeled with anti-J96 antibodies prior to cell permeabilization (external UPEC, blue) and after cell permeabilization (total UPEC, red). This picture shows that Rac1 localizes at the site of bacterial entry and around the internalized bacteria (see Visvikis et al., Traffic 2011 ; 12(5)).
Vol.12 Iss.4, April 2011
Cover Legend: Mycobacterium tuberculosis (M.tb) can inhibit the fusion of lysosomes with its phagosome
This image shows that phagosomes containing M.tb do not fuse with lysosomes even in the macrophage expressing the constitutively active form of Rab7. Macrophage in green, M.tb in bleu, Lysosomes in red. See Seto et al., Traffic 2011; 12(4)).
Vol.12 Iss.3, March 2011
Cover Legend: Three-dimensional model of an Arabidopsis Golgi stack and its trans-Golgi network (TGN) compartments
The model was generated from an electron tomogram of an Arabidopsis root meristem cell that was preserved by high-pressure freezing. In Arabidopsis meristem cells, transformation of a trans-most Golgi cisterna into a TGN cisterna and formation of vesicles from TGN cisternae are often illustrated in multiple TGN cisternae located on the trans-side of Golgi stacks. The Golgi stack in the model image has a Golgi-associated TGN cisterna (aqua) and two free TGN cisternae (silver and red). The TGN cisterna (red) farther from the Golgi has more budding vesicles than the intermediate TGN cisterna (silver) does (see Kang et al., Traffic 2011; 12(3)).
Vol.12 Iss.2, February 2011
Cover Legend: Differential and partial co-localization of Orai1 (green) and STIM1 (white) in polarized secretory cells
The image shows that expression of Orai1 is confined to the apical pole of pancreatic acinar cells close to the tight junctions. By contrast, STIM1 expression is found all along the lateral membrane, including domains that do not express Orai1, where STIM1 is likely to gate other Ca2+ influx channels, most likely TRPC channels (see Hong et al., Traffic 2011; 12(2)).
Vol.12 Iss.1, January 2011
Cover Legend: Quantification of compensatory endocytosis in bovine chromaffin cells
Primary chromaffin cells were transfected with GFP-CD39 to label plasma membrane. Cells were stimulated with nicotine (upper row) and exocytotic sites visualized by anti- Dopamine??-Hydroxylase (DBH) antibodies (red labeling). Subsequent endocytosis was followed by the internalization of anti-DBH antibodies (lower row). Cells were observed by confocal microscopy and cell boundary was delineated based on GFP-CD39 labeling (green). The cell area was converted into Euclidean Distance Map (EDM) that affects a grey value (converted here into a rainbow look-up table) to each pixel according to its distance of the plasma membrane. GFP-CD39 labeling was found between the 2 grey lines depicted on EDM pictures and defined the EDM values for the plasma membrane area. Vesicular structures labeled for DBH were segmented and transformed into regions of interest (ROI, grey circles on EDM pictures). ROI were transferred onto EDM and their mean intensity gave the relative position of DBH positive vesicles. (See Ceridono et al. Traffic 2011; 12(1)).