Cover image for Vol. 17 Issue 6

Edited By: Michael S. Marks, Trina A. Schroer, Tom H. Stevens and Sharon A. Tooze

Online ISSN: 1600-0854

Virtual Issue Autophagy

AutophagyVirtual Issue Autophagy

A Centronuclear Myopathy – Dynamin 2 Mutation Impairs Autophagy in Mice
Anne-Cécile Durieux, Stéphane Vassilopoulos, Jeanne Lainé, Bodvaël Fraysse, Laura Briñas, Bernard Prudhon, Josiane Castells, Damien Freyssenet, Gisèle Bonne, Pascale Guicheney and Marc Bitoun
Traffic, Volume 13, Issue 6: 869–879, Abstract
Dynamin 2 (Dnm2) is involved in endocytosis and intracellular membrane trafficking through its function in vesicle formation from distinct membrane compartments. Heterozygous (HTZ) mutations in the DNM2 gene cause dominant centronuclear myopathy or Charcot–Marie–Tooth neuropathy. We generated a knock-in Dnm2R465W mouse model expressing the most frequent human ...

Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites
Felipe Carlos Martín Zoppino, Rodrigo Damián Militello, Ileana Slavin, Cecilia Álvarez and María I. Colombo
Traffic, Volume 11, Issue 9: 1246–1261, Abstract
Autophagy is an important cellular degradation pathway present in all eukaryotic cells. Via this pathway, portions of the cytoplasm and/or organelles are sequestered in double-membrane structures called autophagosomes. In spite of the significant advance ...

Modulation of Local PtdIns3P Levels by the PI Phosphatase MTMR3 Regulates Constitutive Autophagy
Naoko Taguchi-Atarashi, Maho Hamasaki, Kohichi Matsunaga, Hiroko Omori, Nicholas T. Ktistakis, Tamotsu Yoshimori, Takeshi Noda
Traffic, Volume 11 Issue 4: 468 - 478, Abstract
Autophagy is a catabolic process that delivers cytoplasmic material to the lysosome for degradation. The mechanisms regulating autophagosome formation and size remain unclear. Here, we show that autophagosome formation was triggered by the overexpression of a dominant-negative inactive mutant of Myotubularin-related phosphatase 3 (MTMR3). Mutant MTMR3 partially localized to ...

Death by Committee: Organellar Trafficking and Communication in Apoptosis
Joseph E. Aslan, Gary Thomas
Traffic, Volume 10 Issue 10: 1390 - 1404, Abstract
Apoptosis proceeds through a set of evolutionarily conserved processes that co-ordinate the elimination of damaged or unneeded cells. This program of cell death is carried out by organelle-directed regulators, including the Bcl-2 proteins, and ultimately executed by proteases of the caspase family. Although the biochemical mechanisms of apoptosis are increasingly understood, the underlying ...

Irs4p and Tax4p: Two Redundant EH Domain Proteins Involved in Autophagy
Amandine Bugnicourt, Muriel Mari, Fulvio Reggiori, Rosine Haguenauer-Tsapis, Jean-Marc Galan
Traffic, Volume 9 Issue 5: 755 - 769, Abstract
Proteins carrying EPS15 homology (EH) domains are present from yeast to mammals. The characterized members of this protein family are all involved in intracellular trafficking, typically endocytosis and endocytic recycling. We focused on two members of this family in Saccharomyces cerevisiae Irs4p and Tax4p, whose functions are less well characterized. We show that the deletion of IRS4 altered the function of a neighboring gene, VPS51, involved in endocytic ...

A Picky Eater: Exploring the Mechanisms of Selective Autophagy in Human Pathologies
Aniek van der Vaart, Muriel Mari, Fulvio Reggiori
Traffic, Volume 9 Issue 3: 281 - 289, Abstract
Autophagy is a catabolic process conserved among all eukaryotes essential for the cellular and organismal homeostasis. One of the principal roles of this pathway is to maintain an accurate balance between synthesis, degradation and subsequent recycling of cellular components. Under certain conditions, however, cells are also able to modulate autophagy and specifically remove a number of structures that are potentially ...

Induction of Autophagy Promotes Fusion of Multivesicular Bodies with Autophagic Vacuoles in K562 Cells
Claudio M. Fader, Diego Sánchez, Marcelo Furlán, María I. Colombo
Traffic, Volume 9 Issue 2: 230 - 250, Abstract
Morphological and biochemical studies have shown that autophagosomes fuse with endosomes forming the so-called amphisomes, a prelysosomal hybrid organelle. In the present report, we have analyzed this process in K562 cells, an erythroleukemic cell line that generates multivesicular bodies (MVBs) and releases the internal vesicles known as exosomes into the extracellular medium. We have previously shown ...