FEBS Journal

Cover image for Vol. 281 Issue 8

Edited By: Seamus Martin

Impact Factor: 4.25

ISI Journal Citation Reports © Ranking: 2012: 74/290 (Biochemistry & Molecular Biology)

Online ISSN: 1742-4658

Molecular Enzymology

May 2012 Molecular Enzymology

Papers selected by Nigel Scrutton

Front cover: Figure 2 from Humble et al. (2012) FEBS Journal, 279: 779–792; Figure 2 from Macheroux et al. (2011) FEBS Journal, 278: 2625–2634; Figure 2 from Marrott et al. (2012) FEBS Journal, 279: 713–723; and figure courtesy of Nigel Scrutton

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Enzymes are superb catalysts. Understanding how they achieve large rate enhancements (up to 1021 over the uncatalysed reaction), coordinate complex reaction cycles and attain high levels of reaction specificity remain major challenges in the field. The study of enzymes and their mechanisms requires input from structural biology, kinetics and spectroscopy, computation and engineering as well as more specialized areas (e.g. single molecule methods, laser spectroscopy and chemical intervention). The FEBS Journal publishes high quality papers that embrace these approaches, revealing intricate details of the structures and dynamics of Nature's catalysts. The following selection of papers was made from FEBS Journal articles and reviews published between April 2011 and April 2012. This Virtual Issue showcases some of the excellent contributions published by FEBS Journal in the flourishing field of Molecular Enzymology.

Comparison of the structural changes in two cellobiohydrolases, CcCel6A and CcCel6C, from Coprinopsis cinerea – a tweezer-like motion in the structure of CcCel6C
Mizuki Tamura, Takatsugu Miyazaki, Yutaro Tanaka, Makoto Yoshida, Atsushi Nishikawa and Takashi Tonozuka
FEBS Journal, Volume 279, Issue 10, May 2012, Pages 1871–1882

Characterization of Cupriavidus metallidurans CYP116B1 – A thiocarbamate herbicide oxygenating P450–phthalate dioxygenase reductase fusion protein
Ashley J. Warman, Jacob W. Robinson, Dominika Luciakova, Andrew D. Lawrence, Ker R. Marshall, Martin J. Warren, Myles R. Cheesman, Stephen E. J. Rigby, Andrew W. Munro and Kirsty J. McLean
FEBS Journal, Volume 279, Issue 9, May 2012, Pages 1675–1693

Crystal structure of a ring-cleaving cyclohexane-1,2-dione hydrolase, a novel member of the thiamine diphosphate enzyme family
Alma Steinbach, Sonja Fraas, Jens Harder, Eberhard Warkentin, Peter M. H. Kroneck and Ulrich Ermler
FEBS Journal, Volume 279, Issue 7, April 2012, Pages 1209–1219

FMN fluorescence in inducible NOS constructs reveals a series of conformational states involved in the reductase catalytic cycle
Dipak K. Ghosh, Krishanu Ray, Albert J. Rogers, Nicholas J. Nahm and John C. Salerno
FEBS Journal, Volume 279, Issue 7, April 2012, Pages 1306–1317

Role of polar and nonpolar residues at the active site for PPIase activity of FKBP22 from Shewanella sp. SIB1
Cahyo Budiman, Takashi Tadokoro, Clement Angkawidjaja, Yuichi Koga and Shigenori Kanaya
FEBS Journal, Volume 279, Issue 6, March 2012, Pages 976–986

The catalytic core of an archaeal 2-oxoacid dehydrogenase multienzyme complex is a 42-mer protein assembly
Nia L. Marrott, Jacqueline J. T. Marshall, Dmitri I. Svergun, Susan J. Crennell, David W. Hough, Michael J. Danson and Jean M. H. van den Elsen
FEBS Journal, Volume 279, Issue 5, March 2012, Pages 713–723

Crystal structures of the Chromobacterium violaceumω-transaminase reveal major structural rearrangements upon binding of coenzyme PLP
Maria S. Humble, Karim E. Cassimjee, Maria Håkansson, Yengo R. Kimbung, Björn Walse, Vahak Abedi, Hans-Jürgen Federsel, Per Berglund and Derek T. Logan
FEBS Journal, Volume 279, Issue 5, March 2012, Pages 779–792

Redesign of coenzyme B12 dependent diol dehydratase to be resistant to the mechanism-based inactivation by glycerol and act on longer chain 1,2-diols
Mamoru Yamanishi, Koichiro Kinoshita, Masaki Fukuoka, Takuya Saito, Aya Tanokuchi, Yuuki Ikeda, Hirokazu Obayashi, Koichi Mori, Naoki Shibata, Takamasa Tobimatsu and Tetsuo Toraya
FEBS Journal, Volume 279, Issue 5, March 2012, pages 793–804

Mechanism for folate-independent aldolase reaction catalyzed by serine hydroxymethyltransferase
Yoko Chiba, Tohru Terada, Masafumi Kameya, Kentaro Shimizu, Hiroyuki Arai, Masaharu Ishii and Yasuo Igarashi
FEBS Journal, Volume 279, Issue 3, February 2012, Pages 504–514

The ‘gating’ residues Ile199 and Tyr326 in human monoamine oxidase B function in substrate and inhibitor recognition
Erika M. Milczek, Claudia Binda, Stefano Rovida, Andrea Mattevi and Dale E. Edmondson
FEBS Journal, Volume 278, Issue 24, December 2011, Pages 4860–4869

Is rat an appropriate animal model to study the involvement of d-serine catabolism in schizophrenia? insights from characterization of d-amino acid oxidase
Luca F. Frattini, Luciano Piubelli, Silvia Sacchi, Gianluca Molla and Loredano Pollegioni
FEBS Journal, Volume 278, Issue 22, November 2011, Pages 4362–4373

A kinetic model linking protein conformational motions, interflavin electron transfer and electron flux through a dual-flavin enzyme – simulating the reductase activity of the endothelial and neuronal nitric oxide synthase flavoprotein domains
Mohammad M. Haque, Claire Kenney, Jesús Tejero and Dennis J. Stuehr
FEBS Journal, Volume 278, Issue 21, November 2011, Pages 4055–4069

Characterization of the PLP-dependent aminotransferase NikK from Streptomyces tendae and its putative role in nikkomycin biosynthesis
Alexandra Binter, Gustav Oberdorfer, Sebastian Hofzumahaus, Stefanie Nerstheimer, Georg Altenbacher, Karl Gruber and Peter Macheroux
FEBS Journal, Volume 278, Issue 21, November 2011, Pages 4122–4135

Crystal structure of Onconase at 1.1 Å resolution – insights into substrate binding and collective motion
Daniel E. Holloway, Umesh P. Singh, Kuslima Shogen and K. Ravi Acharya
FEBS Journal, Volume 278, Issue 21, November 2011, Pages 4136–4149

Structural characterization of angiotensin I-converting enzyme in complex with a selenium analogue of captopril
Mohd Akif, Geoffrey Masuyer, Sylva L. U. Schwager, Bhaskar J. Bhuyan, Govindasamy Mugesh, R. Elwyn Isaac, Edward D. Sturrock and K. Ravi Acharya
FEBS Journal, Volume 278, Issue 19, October 2011, Pages 3644–3650

Selective hydroxylation of alkanes by an extracellular fungal peroxygenase
Sebastian Peter, Matthias Kinne, Xiaoshi Wang, René Ullrich, Gernot Kayser, John T. Groves and Martin Hofrichter
FEBS Journal, Volume 278, Issue 19, October 2011, pages 3667–3675

Substrate-induced conformational changes in Plasmodium falciparum guanosine monophosphate synthetase
Javaid Y. Bhat, Roopa Venkatachala and Hemalatha Balaram
FEBS Journal, Volume 278, Issue 19, October 2011, Pages 3756–3768

Flavogenomics – a genomic and structural view of flavin-dependent proteins
Peter Macheroux, Barbara Kappes and Steven E. Ealick
FEBS Journal, Volume 278, Issue 15, August 2011, pages 2625–2634

Insight into the phosphodiesterase mechanism from combined QM/MM free energy simulations
Kin-Yiu Wong and Jiali Gao
FEBS Journal, Volume 278, Issue 14, July 2011, Pages 2579–2595

Dynamics in electron transfer protein complexes
Qamar Bashir, Sandra Scanu and Marcellus Ubbink
FEBS Journal, Volume 278, Issue 9, May 2011, Pages 1391–1400

Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases
Francesca Esposito, Tatyana Kharlamova, Simona Distinto, Luca Zinzula, Yung-Chi Cheng, Ginger Dutschman, Giovanni Floris, Patrick Markt, Angela Corona and Enzo Tramontano
FEBS Journal, Volume 278, Issue 9, May 2011, Pages 1444–1457

A kinetic model for the burst phase of processive cellulases
Eigil Praestgaard, Jens Elmerdahl, Leigh Murphy, Søren Nymand, K. C. McFarland, Kim Borch and Peter Westh
FEBS Journal, Volume 278, Issue 9, May 2011, pages 1547–1560

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