Virtual Issue Molecular Enzymology

November 2008

Papers Selected by Nigel Scrutton

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Introduction

Modern enzymology research is interdisciplinary and endeavours to elucidate the relationship between structure, dynamics and chemistry and relate this to metabolic activity within the cell. The aim is to elucidate, at various levels, mechanistic details of biomolecular processes, ranging from complex multi-component pathways and complexes to single proteins (enzymes), either through ensemble measurements, or at the level of isolated (single) molecules. It is an established but rapidly evolving field, uniting structural and computational approaches with analysis of reaction dynamics, engineering, spectroscopy, chemical analysis and theory. Historically, FEBS Journal has encouraged the submission of high quality papers in the field of enzymology and is particularly keen to publish work that reflects the strong interdisciplinary basis of the field. This selection of recent papers reflects this aim and underscores the high quality work published by FEBS Journal in the field of enzymology.

• Structure-guided alteration of coenzyme specificity of formate dehydrogenase by saturation mutagenesis to enable efficient utilization of NADP
  Aggliki Andreadeli, Dimitris Platis, Vladimir Tishkov, Vladimir Popov, Nikolaos Labrou
  FEBS Journal Volume 275, Issue 15, August 2008, Pages: 3859-69
• Importance of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and L-allo-Thr cleavage: crystal structure and biochemical studies
  B. S. Bhavani, V. Rajaram, Shveta Bisht, Purnima Kaul, V. Prakash, M. R. N. Murthy, N. Appaji Rao, H. S. Savithri
  FEBS Journal Volume 275, Issue 18, September 2008, Pages: 4606-19
• Hexameric Ring Structure of the N-terminal Domain of Mycobacterium tuberculosis DnaB Helicase
  Tapan Biswas, Oleg Tsodikov
  FEBS Journal Volume 275, Issue 12, June 2008, Pages: 3064-71
• Inter-flavin electron transfer in cytochrome P450 reductase – effects of solvent and pH identify hidden complexity in mechanism
  Sibylle Brenner, Sam Hay, Andrew Munro, Nigel Scrutton
  FEBS Journal Volume 275, Issue 18, September 2008, Pages: 4540-57
• An intermediate step in the evolution of ATPases – a hybrid F_O-V_O rotor in a bacterial Na F₁F_O ATP synthase
  Michael Fritz, Adriana Klyszejko, Nina Morgner, Janet Vonck, Bernd Brutschy, Daniel Muller, Thomas Meier, Volker Muller
  FEBS Journal Volume 275, Issue 9, May 2008, Pages: 1999-2007
• Covalent flavinylation of vanillyl-alcohol oxidase is an autocatalytic process
  Jianfeng Jin, Hortense Mazon, Robert H.H. van den Heuvel, Dick B. Janssen, Albert J. Heck, Marco Fraaije
  FEBS Journal Volume 275, Issue 20, October 2008, Pages: 5191-5200
• A cysteine residue near the propionate side-chain of heme is the radical site in ascorbate peroxidase
  Sakhito Kitajima, Miyo Kurioka, Tadashi Yoshimoto, Mayumi Shindo, Kenji Kanaori, Kunihiko Tajima, Kenji Oda
  FEBS Journal Volume 275, Issue 3, February 2008, Pages: 470-80
• Characterization of mutations in crucial residues around the Q_o binding site of the cytochrome bc1 complex from Paracoccus denitrificans
  Thomas Kleinschroth, Oliver Anderka, Michaela Ritter, Andreas Stockera, Thomas A. Link, Bernd Ludwig, Petra Hellwig
  FEBS Journal Volume 275, Issue 19, October 2008, Pages: 4773-85
• Directed evolution of a histone acetyltransferase – enhancing thermostability, whilst maintaining catalytic activity and substrate specificity
  Hans Leemhuis, Karl P. Nightingale, F Hollfelder
  FEBS Journal Volume 275, Issue 22, November 2008, Pages: 5635-47
• Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica-polymerase and silica-esterase)
  Werner Muller, Ute Schlobmacher, Xiaohung Wang, Alexandra Boreiko, David Brandt, Stephan Wolf, Wolfgang Tremal, Heinz Schroder
  FEBS Journal Volume 275, Issue 2, January 2008, Pages: 362-70
• Heavy metal ions inhibit molybdoenzyme activity by binding to the dithiolene moiety of molybdopterin in Escherichia coli
  Meina Neumann, Silke Leimkühler
  FEBS Journal Volume 275, Issue 22, November 2008, Pages: 5678-89
• Influence of modulated structural dynamics on the kinetics of a-chymotrypsin catalysis. Insights through chemical glycosylation, molecular dynamics, and domain motion analysis
  Ricardo J. Sola, Kai Griebenow
  FEBS Journal Volume 273, Issue 23, December 2006, Pages: 5303-19
  
• Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis
  Ekaterina Morganova, Boris Illarionov, Thota Sambaiah, Ilka Haase, Adelbert Bacher, Mark Cushman, Markus Fischer, Rudolf Ladenstein
  FEBS Journal Volume 273, Issue 20, October 2006, Pages: 4790-804
• Reactivity of the heme–dioxygen complex of the inducible nitric oxide synthase in the presence of alternative substrates
  David Lefevre-Groboillot, Jean-Luc Boucher, Daniel Mansuy, Dennis Stuehr
  FEBS Journal Volume 273, Issue 1, January 2006, Pages: 180-91
• Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus
  Robert Solomon
  FEBS Journal Volume 272, Issue 1, January 2005, Pages: 259-68
• Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme
  Hans Eklund, Urszula Kosinska , Cecilia Carnrot, Staffan Eriksson, Liya Wang
  FEBS Journal Volume 272, Issue 24, December 2005, Pages: 6365-72
• ATP-dependent ligases in trypanothione biosynthesis – kinetics of catalysis and inhibition by phosphinic acid pseudopeptides
  Sandra L. Oza, Shoujun Chen, Susan Wyllie, James K. Coward, Alan Fairlamb
  FEBS Journal Volume 275, Issue 21, November 2008, Pages: 5408-21
• Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity
  Gernot Rauch, Heidemarie Ehammer, Stephen Bornemann, Peter Macheroux
  FEBS Journal Volume 275, Issue 7, April 2008, Pages: 1464-73
• Kinetic characterization of methionine [gamma]-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis
  Dan Sato, Wataru Yamagata, Shigeharu Harada, Tomoyoshi Nozaki
  FEBS Journal Volume 275, Issue 3, February 2008, Pages: 548-60
• The role of group bulkiness in the catalytic activity of psychrophile cold-active protein tyrosine phosphatase
  Hiroki Tsuruta , Bunzo Mikami, Chiaki Yamamoto, Hiroshi Yamagata
  FEBS Journal Volume 275, Issue 17, September 2008, Pages: 4317-28
• Spectroscopic characterization of oxyferrous complex of prostacyclin synthase in solution and in trapped sol-gel matrix
  Hui-Chun Yeh, Pei-Yung Hsu, Ah-Lim Tsai, Lee-Ho Wang
  FEBS Journal Volume 275, Issue 9, May 2008, Pages: 2305-14

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