FEBS Journal

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Edited By: Seamus Martin

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Online ISSN: 1742-4658

Virtual Issue on Glycosylation

Virtual Issue Glycosylation

February 2012

Papers selected by: Dan Michele

Front cover: Figure 1 from Aich (2011) FEBS J 278: 1699-1712; Figure 6 from S. Pouilly et al (2012) FEBS J 279: 586-598; Figure 3 from E J A Veldhuizen et al (2011) FEBS J 278: 3930-3941

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To some, carbohydrate modifications of proteins are an annoying nuisance that prevents functional recombinant protein expression or complicates normal protein purification and characterization. To others, the complex, branched stereospecific linkages of glycans are beautiful puzzles to decode and study their function. The field of glycobiology continues to expand at an ever increasing rate. My college biochemistry textbook written in 1990, although touting the great accomplishments of the field in the prior 20 years, could only give a few classic examples of glycoprotein modifications with known function such as the role of proteoglycans in cartilage, the protective role of mucins in epithelium, and antifreeze glycoproteins of arctic fishes. That 1990 textbook in fact indicated that the function of the carbohydrates moieties on most glycoproteins remained “enigmatic”.

While many questions still remain, carbohydrate modifications of proteins have begun to lose their “enigmatic” classification. With the decoding of the human genome and a number of genomes from other species in the last decade and the ever-expanding toolbox to study the chemistry of glycan moieties, the rich diversity of protein glycosylation, the function of the enzymes mediating these beautiful functional decorations on proteins, and the critical role glycosylation in normal biology and human disease is becoming increasingly evident. The identification of important human genetic diseases caused by abnormal glycosylation, such as the multi-syndrome Congenital Disorders of Glycosylation, several forms of muscular dystrophy, glycosaminoglycan diseases such as Ehlers-Danlos Syndrome and chondrodysplasias, and many others, have pointed to the critical role of glycosylation in human health and disease. Furthermore, while many membrane proteins utilize glycans to interact with their extracellular environment, many pathogenic organisms, such as influenza, hemorrhagic fever viruses, and bacteria causing leprosy, hijack these same interactions to gain entry into cells. The growing diverse list of the roles of glycans in important human diseases makes the study of their synthesis, structure, and impact on protein and cellular function critical to our understanding of pathogenic mechanisms.

Our journal’s submissions in the field of glycobiology have reflected this rich diversity, with papers ranging from the study of the roles of glycoproteins in human disease and cellular biology, to explaining how glycans affect the production of the smell of ripe tomatoes (Louveau et al FEBSJ 2011), and the function of hormones in stick bugs (Munte et al FEBSJ 2008). To fully capture this diversity of manuscripts in a single Virtual Issue is impossible. Therefore, this Issue highlights recent papers in the journal demonstrating:

1) how state-of-the-art high-throughput and high-sensitivity glycomic approaches are deciphering new glycomic profiles to shed light on biological mechanisms and pathomechanisms of disease.

2) the mechanisms of how important cellular and protein functions are directly mediated by post-translational protein glycosylation

3) the translation of these newly found mechanisms toward novel “glyco- therapies” for treating important human diseases

Glycomic approaches: new insight into glycan diversity in health and disease

Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid
Adishesha Puneeth Kumar, Chilkunda D. Nandini, Paramahans V. Salimath
FEBS Journal, Volume 278, Issue 1, January 2011, Pages: 143–155

Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues
Cees Bruggink, Ben J. H. M. Poorthuis, Monique Piraud, Roseline Froissart, André M. Deelder, Manfred Wuhrer
FEBS Journal, Volume 277, Issue 14, July 2010, Pages: 2970–2986

Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay
Udayanath Aich, Nia Beckley, Zachary Shriver, Rahul Raman, Karthik Viswanathan, Sven Hobbie, Ram Sasisekharan
FEBS Journal, Volume 278, Issue 10, May 2011, Pages: 1699–1712

Alteration of the N-glycome of bovine milk glycoproteins during early lactation
Shota Takimori, Hideyuki Shimaoka, Jun-Ichi Furukawa, Tadashi Yamashita, Maho Amano, Naoki Fujitani, Yasuhiro Takegawa, Lennart Hammarström, Imre Kacskovics, Yasuro Shinohara, Shin-Ichiro Nishimura
FEBS Journal, Volume 278, Issue 19, October 2011, Pages: 3769–3781

Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans
Rikio Yabe, Hiroaki Tateno, Jun Hirabayashi
FEBS Journal, Volume 277, Issue 19, October 2010, Pages: 4010–4026

A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics
Hisashi Narimatsu, Hiromichi Sawaki, Atsushi Kuno, Hiroyuki Kaji, Hiromi Ito, Yuzuru Ikehara
FEBS Journal, Volume 277, Issue 1, January 2010, Pages: 95–105

Glycans directly mediate protein function and cellular interactions

Mucin-type O-glycosylation – putting the pieces together
Pia H. Jensen, Daniel Kolarich, Nicolle H. Packer
FEBS Journal, Volume 277, Issue 1, January 2010, Pages: 81–94

The carbohydrate recognition domain of collectins
Edwin J. A. Veldhuizen, Martin van Eijk, Henk P. Haagsman
FEBS Journal, Volume 278, Issue 20, October 2011, Pages: 3930–3941

Viral entry mechanisms: cellular and viral mediators of herpes simplex virus entry
Jihan Akhtar, Deepak Shukla
FEBS Journal, Volume 276, Issue 24, December 2009, Pages: 7228–7236

Type I receptor binding of bone morphogenetic protein 6 is dependent on N-glycosylation of the ligand
Stefan Saremba, Joachim Nickel, Axel Seher, Alexander Kotzsch, Walter Sebald, Thomas D. Mueller
FEBS Journal, Volume 275, Issue 1, January 2008, Pages: 172–183

N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding
Lavinia Bhatt, Claire Murphy, Liam S. O’Driscoll, Maria Carmo-Fonseca, Mary W. McCaffrey, John V. Fleming
FEBS Journal, Volume 277, Issue 15, July 2011, Pages: 3219–3234

N-glycosylation influences the structure and self-association abilities of recombinant nucleolin
Marie-Estelle Losfeld, Arnaud Leroy, Bernadette Coddeville, Mathieu Carpentier, Joël Mazurier, Dominique Legrand
FEBS Journal, Volume 278, Issue 14, August 2010, Pages: 2552–2564

Branched N-glycans regulate the biological functions of integrins and cadherins
Yanyang Zhao, Yuya Sato, Tomoya Isaji, Tomohiko Fukuda, Akio Matsumoto, Eiji Miyoshi, Jianguo Gu, Naoyuki Taniguchi
FEBS Journal, Volume 275, Issue 10, May 2008, Pages: 1939–1948

Disruption of N-linked glycosylation enhances ubiquitin-mediated proteasomal degradation of the human ATP-binding cassette transporter ABCG2
Hiroshi Nakagawa, Kanako Wakabayashi-Nakao, Ai Tamura, Yu Toyoda, Shoko Koshiba, Toshihisa Ishikawa
FEBS Journal, Volume 276, Issue 24, December 2009, Pages: 7237–7252

Asparagine-linked glycosylation of human chymotrypsin C is required for folding and secretion but not for enzyme activity
Melinda Bence, Miklós Sahin-Tóth
FEBS Journal, Volume 278, Issue 22, November 2011, Pages: 4338–4350

“Glyco-therapies”: translation from bench to bedside

The pharmacological chaperone isofagomine increases the activity of the Gaucher disease L444P mutant form of β-glucosidase
Richie Khanna, Elfrida R. Benjamin, Lee Pellegrino, Adriane Schilling, Brigitte A. Rigat, Rebecca Soska, Hadis Nafar, Brian E. Ranes, Jessie Feng, Yi Lun, Allan C. Powe, David J. Palling, Brandon A. Wustman, Raphael Schiffmann, Don J. Mahuran, David J. Lockhart, Kenneth J. Valenzano
FEBS Journal, Volume 277, Issue 7, April 2010, Pages: 1618–1638

Proteoglycans in health and disease: novel roles for proteoglycans in malignancy and their pharmacological targeting
Achilleas D. Theocharis, Spyridon S. Skandalis, George N. Tzanakakis, Nikos K. Karamanos
FEBS Journal, Volume 277, Issue 19, October 2010, Pages: 3904–3923

Design and humanization of a murine scFv that blocks human platelet glycoprotein VI in vitro
Julien Muzard, Maxime Bouabdelli, Muhammad Zahid, Véronique Ollivier, Jean Jacques Lacapère, Martine Jandrot-Perrus, Philippe Billiald
FEBS Journal, Volume 276, Issue 15, August 2009, Pages: 4207–4222

Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris
Bassam Al Atalah, Elke Fouquaert, Dieter Vanderschaeghe, Paul Proost, Jan Balzarini, David F. Smith, Pierre Rougé, Yi Lasanajak, Nico Callewaert, Els J. M. Van Damme
FEBS Journal, Volume 278, Issue 12, June 2011, Pages: 2064–2079

Metabolic glycoengineering through the mammalian GalNAc salvage pathway
Sabrina Pouilly, Véronique Piller, Friedrich Piller
FEBS Journal, Volume 279, Issue 4, February 2012, Pages: 586–598

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