Photochemistry and Photobiology

Cover image for Vol. 91 Issue 4

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Jean Cadet

Impact Factor: 2.266

ISI Journal Citation Reports © Ranking: 2014: 44/73 (Biophysics); 184/289 (Biochemistry & Molecular Biology)

Online ISSN: 1751-1097


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  1. Research Articles

    1. Impact of Substituents in Tumor Uptake and Fluorescence Imaging Ability of Near-Infrared Cyanine-like Dyes

      Nayan J. Patel, Ethirajan Manivannan, Penny Joshi, Tymish J. Ohulchanskyy, Roger R. Nani, Martin J. Schnermann and Ravindra K. Pandey

      Article first published online: 30 JUL 2015 | DOI: 10.1111/php.12482

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      Impact of substituents in tumor uptake and fluorescence imaging ability of near-infrared heterocyclic polymethine dyes.

    2. Effects of Light and Salinity Stresses in Production of Mycosporine-Like Amino Acids by Gymnodinium catenatum (Dinophyceae)

      Paulo Vale

      Article first published online: 30 JUL 2015 | DOI: 10.1111/php.12488

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      Mycosporine-like amino acids concentration increased with salinity, in particular high polar MAAs. Abrupt changes in light intensity increased rapidly the low polar palythene. In both cases, mycosporine–glycine was one of the MAAs with the greatest increase, while biosynthesis of M370, a major MAA in G. catenatum, progressed slowly. Experiments put into evidence the biosynthetic relations between MAAs and its degradation pathways. MAAs might play a multifunctional role to cope with salinity (shinorine, porphyra-334) and photooxidative (mycosporine–glycine, palythene, M-370) stresses. High salinity and high light conditions also altered chain formation toward shorter chains or solitary cells.

  2. Letters to the Editor

  3. Research Articles

    1. Effectiveness of the Photoactive Dye Methylene Blue versus Caspofungin on the Candida parapsilosis Biofilm in vitro and ex vivo

      Lucia Černáková, Jaroslava Chupáčová, Katarína Židlíková and Helena Bujdáková

      Article first published online: 24 JUL 2015 | DOI: 10.1111/php.12480

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      PDI (photodynamic inactivation) is useful approach for eradication of microorganisms. This work compared effectiveness of methylene blue (MB) with clinically used drug – caspofungin (CAS). Biofilms of Candida parapsilosis were formed in 96-well microtiter plates and irradiated with red LED light (15 J cm−2) for 2.5 h after addition of different MB concentrations. Evaluation of results using CFU and XTT reduction assay showed effectiveness of PDI in contrast to CAS. Microscopic examination of in vitro and ex vivo biofilms formed on mouse tongues confirmed effect of MB on C. parapsilosis strains in the concentration range of 0.7–1.35 mmol L−1.

  4. Special Issue Research Articles

    1. Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells

      María Albana Di Palma, María Gabriela Alvarez and Edgardo N. Durantini

      Article first published online: 24 JUL 2015 | DOI: 10.1111/php.12483

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      The photoreaction type I/type II pathways mediated by ZnPPc4+ was studied in Candida albicans cells. Photoinactivation was investigated in presence of specific molecular probes to detect the presence of ROS, under different experimental conditions and in presence of scavengers of ROS to find information about the photoprocesses that produces the cell death. These investigation indicates that O2(1Δg) is generated in the cells, although a minor extension other radical species can also be involved in the photoinactivation of C. albicans mediated by ZnPPc4+.

  5. Research Articles

    1. Effect of 808 nm Diode Laser on Swimming Behavior, Food Vacuole Formation and Endogenous ATP Production of Paramecium primaurelia (Protozoa)

      Andrea Amaroli, Silvia Ravera, Steven Parker, Isabella Panfoli, Alberico Benedicenti and Stefano Benedicenti

      Article first published online: 24 JUL 2015 | DOI: 10.1111/php.12486

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      Photobiomodulation and Low-Level Laser Therapy are terms applied to the manipulation of cellular behavior using low-intensity light sources and work on the principle of inducing a biological response through energy transfer. In our work, the effect of laser therapy by 808-nm infrared diode laser with a flat-top handpiece (1 W in CW–64 J cm−2) on Paramecium (Protozoa) has been investigated. The therapy increases Paramecium's swimming speed and food vacuole formation and positively affects adenosine triphosphate (ATP) production. The increment of ATP without a thermal effect highlights the positive photobiomodulating effect of the therapy and the optimal irradiation conditions by the flat-top handpiece.

    2. Enhanced Self-Cleaning Properties on Polyester Fabric Under Visible Light Through Single-Step Synthesis of Cuprous Oxide Doped Nano-TiO2

      Hamdam Gaminian and Majid Montazer

      Article first published online: 20 JUL 2015 | DOI: 10.1111/php.12478

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      Here, Cu2O-doped TiO2 nanoparticles were synthesized simultaneous with alkali hydrolysis on the polyester fabric through a simple sonochemical technique. A statistical method namely central composite design was used to determine the effect of three different variables including CuSO4.5H2O, glucose and pH on the self-cleaning properties and weight of the fabric. FE-SEM images and XRD/EDX patterns confirmed formation of Cu2O-doped TiO2 nanoparticles on the polyester fabric. The treated polyester fabric indicated superior durable self-cleaning properties along with high tensile strength.

    3. Reclamation of Water Polluted with Flubendiamide Residues by Photocatalytic Treatment with Semiconductor Oxides

      José Fenoll, Nuria Vela, Isabel Garrido, Ginés Navarro, Gabriel Pérez-Lucas and Simón Navarro

      Article first published online: 17 JUL 2015 | DOI: 10.1111/php.12479

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      The degradation of flubendiamide in aqueous suspensions of binary (ZnO and TiO2) and ternary (Zn2TiO4 and ZnTiO3) oxides of zinc and titanium under artificial light (300–460 nm) irradiation has been studied. The results show that the use of photosensitizer/Na2S2O8, especially with TiO2 and ZnO, is a very effective and rapid method for the elimination of this pesticide in water. In addition, the main photocatalytic intermediate (desiodo-flubendiamide) detected during the degradation of flubendiamide was identified. These results have high environmental interest for the treatment of polluted water and therefore a significant scientific impact.

    4. The Effect of Surface Charge Saturation On Heat-induced Aggregation of Firefly Luciferase

      Jamileh Gharanlar, Saman Hosseinkhani, Reza H. Sajedi and Parichehr Yaghmaie

      Article first published online: 14 JUL 2015 | DOI: 10.1111/php.12467

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      The effect of surface charge saturation on heat-induced aggregation of firefly luciferase. Different surface charge distribution has an effect on thermal aggregation of mutant luciferases at 45°C.

    5. Effects of UV-C and Vacuum-UV TiO2 Advanced Oxidation Processes on the Acute Mortality of Microalgae

      Eric McGivney, Magnus Carlsson, Jon Petter Gustafsson and Elena Gorokhova

      Article first published online: 2 JUL 2015 | DOI: 10.1111/php.12473

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      In response to the transport of invasive species through ballast tanks, the shipping industry has begun employing disinfection treatment systems. This study compares the efficiencies of common treatment systems that employ TiO2 and different UV wavelengths on a freshwater alga (Pseudokirchneriella subcapitata) and a marine alga (Tetraselmis suecica). Treatment efficacy increased in the presence of TiO2 and when Vacuum UV light was added as a part of the irradiation. However, the effects were dependent on the algal species used as test organisms.

    6. Measuring the Physiologic Properties of Oral Lesions Receiving Fractionated Photodynamic Therapy

      Shannon M. Gallagher-Colombo, Harry Quon, Kelly M. Malloy, Peter H. Ahn, Keith A. Cengel, Charles B. Simone II, Ara A. Chalian, Bert W. O'Malley, Gregory S. Weinstein, Timothy C. Zhu, Mary E. Putt, Jarod C. Finlay and Theresa M. Busch

      Article first published online: 2 JUL 2015 | DOI: 10.1111/php.12475

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      Diffuse reflectance spectroscopy with a contact probe was employed as part of a fluorescence and reflectance spectroscopy system to measure the tissue hemoglobin oxygen saturation and hemoglobin content of lesions of premalignant or early microinvasive cancer of the oral cavity. Studies demonstrate the feasibility of incorporating these measurements into treatment with fractionated (two-part) photodynamic therapy (PDT) using 5-aminolevulinic acid. Patient-specific differences in physiologic parameters were detectable at baseline and at times during and after PDT. Photobleaching of photosensitizer was measured by its fluorescence. Results establish the utility of rationally designed spectroscopy probes toward personalized dosimetry in PDT of oral disease.

    7. Kinetic Model of Photoautotrophic Growth of Chlorella sp. Microalga, Isolated from the Setúbal Lagoon

      Josué Miguel Heinrich and Horacio Antonio Irazoqui

      Article first published online: 29 JUN 2015 | DOI: 10.1111/php.12468

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      Photosynthesis and Microalgal growth rate depends on the local light availability; in this work a kinetic expression for algal growth under light controlling conditions has been derived from a simplified pathway of the light-dependent step of photosynthesis.

    8. Photophysics and Rotational Dynamics of a Hydrophilic Molecule in a Room Temperature Ionic Liquid

      Aninda Chatterjee, Banibrata Maity, Sayeed Ashique Ahmed and Debabrata Seth

      Article first published online: 24 JUN 2015 | DOI: 10.1111/php.12472

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      This paper focuses on the photophysics and rotational diffusion of a hydrophilic solute 7-(N, N′-diethylamino)coumarin-3-carboxylic acid (7-DCCA) in a room temperature ionic liquid methyltrioctylammonium bis(trifluoromethylsulfonyl) imide ([N1888][NTf2]).

    9. Doping of Zinc Oxide with Selected First Row Transition Metals for Photocatalytic Applications

      Darina Schelonka, Jakub Tolasz and Václav Štengl

      Article first published online: 24 JUN 2015 | DOI: 10.1111/php.12469

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      ZnO samples doped with selected first row transition metals were used for degradation of an organic dye Reactive Black 5. The results of the photocatalytic experiments showed that the samples were active under VIS light. The most photocatalytically active was the Fe-doped ZnO.

    10. The Synthesis and Photocatalytic Properties of TiO2 Nanotube Array by Starch-Modified Anodic Oxidation

      Fengjun Zhang, Zijian Liu, Wei Lu, Cong Lyu, Chuan Lyu and Xiansheng Wang

      Article first published online: 17 JUN 2015 | DOI: 10.1111/php.12471

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      The TiO2 nanotube arrays were prepared by anodization process with starch addition. With the starch addition, the viscosity of the electrolyte was controlled, which modified the prepared nanotubes grew completely. The as-prepared nanotubes possessed well uniformed and higher photodegradation responsive than the pure TiO2, and consequently enhancing the photochromic properties.

    11. Estimating the Diurnal Cycle and Daily Insolation of Ultraviolet and Photosynthetically Active Radiation at the Sea Surface

      Victor S. Kuwahara and Satoru Taguchi

      Article first published online: 17 JUN 2015 | DOI: 10.1111/php.12474

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      The diurnal variability of in situ surface (0+) and subsurface (0) ultraviolet radiation and photosynthetically active radiation were measured, modeled and tested. The diurnal variability in UVR and PAR were wavelength dependent and were modeled by a simple sinusoidal equation. Higher n values in the UV-B spectrum suggest sharper increase/decrease near sunrise and sunset hours, ultimately reducing the daily insolation at specific wavelengths and altering the UV-B/(UV-A + PAR) ratio throughout the day.

  6. Invited Reviews

    1. You have free access to this content
      How Does Photoreceptor UVR8 Perceive a UV-B Signal?

      Xiaojing Yang, Sherwin Montano and Zhong Ren

      Article first published online: 11 JUN 2015 | DOI: 10.1111/php.12470

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      Light perception of UV-B photoreceptor UVR8 is triggered by light-induced molecular events originating in two epicenters located at the dimer interface. Clustered tryptophan residues serve as UV-B pigments and undergo concerted conformational changes upon absorbing UV-B photons, which disrupt the dimer interface and lead to dimer dissociation of UVR8.

    2. You have free access to this content
      Green Light to Plant Responses to Pathogens: The Role of Chloroplast Light-Dependent Signaling in Biotic Stress

      María Laura Delprato, Adriana R. Krapp and Néstor Carrillo

      Article first published online: 11 JUN 2015 | DOI: 10.1111/php.12466

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      The effect of light on ROS production by chloroplasts during a non-host pathogen interaction causing LCD is illustrated by infiltrating tobacco leaves with Xanthomonas campestris (see reference [16] in the text). Leaves were incubated with a ROS-reacting probe and observed 19 h post-infiltration by confocal laser microscopy, merging bright field, chlorophyll self-fluorescence (red), and ROS fluorescence (green). In leaves kept in the dark (left), chloroplasts were visualized as a red necklace around the central vacuole. Illuminated tissue (right) shows chloroplasts in yellow due to combination of red and green fluorescence, indicating that pathogen interaction results in light-dependent plastid ROS production.


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