Measuring the Physiologic Properties of Oral Lesions Receiving Fractionated Photodynamic Therapy
Shannon M. Gallagher-Colombo, Harry Quon, Kelly M. Malloy, Peter H. Ahn, Keith A. Cengel, Charles B. Simone II, Ara A. Chalian, Bert W. O'Malley, Gregory S. Weinstein, Timothy C. Zhu, Mary E. Putt, Jarod C. Finlay and Theresa M. Busch
Article first published online: 2 JUL 2015 | DOI: 10.1111/php.12475
Diffuse reflectance spectroscopy with a contact probe was employed as part of a fluorescence and reflectance spectroscopy system to measure the tissue hemoglobin oxygen saturation and hemoglobin content of lesions of premalignant or early microinvasive cancer of the oral cavity. Studies demonstrate the feasibility of incorporating these measurements into treatment with fractionated (two-part) photodynamic therapy (PDT) using 5-aminolevulinic acid. Patient-specific differences in physiologic parameters were detectable at baseline and at times during and after PDT. Photobleaching of photosensitizer was measured by its fluorescence. Results establish the utility of rationally designed spectroscopy probes toward personalized dosimetry in PDT of oral disease.