PDT Dose Parameters Impact Tumoricidal Durability and Cell Death Pathways in a 3D Ovarian Cancer Model
Imran Rizvi, Sriram Anbil, Nermina Alagic, Jonathan P. Celli, Lei Zak Zheng, Akilan Palanisami, Michael D. Glidden, Brian W. Pogue and Tayyaba Hasan
Article first published online: 4 APR 2013 | DOI: 10.1111/php.12065
The photosensitizer-light parameters used to construct a photodynamic therapy (PDT) dose significantly impact tumor destruction and regrowth kinetics. A 3D ovarian cancer model (a) is used to evaluate a matrix of benzoporphyin derivative (BPD) and light combinations for three fixed PDT doses (b). PDT with 0.25 μM BPD (blue) provides maximum cytotoxic durability for all doses (c). Representative LIVE/DEAD (green/red) images (d) that were used to generate normalized viabilities in (c), depict increased killing in nodules treated with 0.25 μM BPD-PDT (2) compared to PDT with 1 μM (3) or 10 μM (4) BPD. These findings suggest that customization of PDT parameters could improve treatment outcomes.