Photochemistry and Photobiology

Cover image for Vol. 93 Issue 6

Edited By: Jean Cadet

Impact Factor: 2.121

ISI Journal Citation Reports © Ranking: 2016: 47/73 (Biophysics); 201/290 (Biochemistry & Molecular Biology)

Online ISSN: 1751-1097

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  • Phycoviolobilin (PVB) formation and spectral tuning in NpR6012g4 variants

    Phycoviolobilin (PVB) formation and spectral tuning in NpR6012g4 variants

    Phycoviolobilin (PVB) formation and spectral tuning in NpR6012g4 variants. (A) Absorption spectra are shown for H659C L660F (DTCFQ) NpR6012g4 after illumination with the indicated colors of light. (B) Normalized photochemical difference spectra are shown for denatured DTCLQ NpR6012g4 (blue), DTCFQ NpR6012g4 (green) and A622L DTCFQ NpR6012g4 (dusty rose) after illumination with red (650 ± 20 nm), violet (400 ± 35 nm), and green (550 ± 35 nm) light, respectively. (C) Absorption spectra are shown for A622L H659C L660F (A622L DTCFQ) NpR6012g4 before (15Z, blue trace) and after (15E, orange trace) illumination with green light. (D) Normalized 15Z – 15E difference spectra are shown for Anacy_3174g6 (cyan) and A622L DTCFQ NpR6012g4 (dusty rose). (E) Normalized absorption spectra are shown for wild-type NpR6012g4 (dark blue trace), DTCFQ NpR6012g4 (green trace), A622L NpR6012g4 (dusty rose trace), and NpR5113g3 (dark red trace) in the 15Z state after acid denaturation. Wild-type NpR6012g4 contains a phycocyanobilin (PCB) adduct, whereas NpR5113g3 contains a PVB adduct .

  • Formation of reactive oxygen species (ROS) and subsequent cell killing

    Formation of reactive oxygen species (ROS) and subsequent cell killing

    Schematic showing the combination of light, sensitizer and oxygen that results in the formation of reactive oxygen species (ROS) and subsequent cell killing.

  • Absorption spectra

    Absorption spectra

    Absorption spectra (normalized at the B band) in toluene for tolyporphin A (A), synthetic bacteriochlorins (B), native chlorins (C) and native bacteriochlorins (D).

  • Photodynamic therapy (PDT) efficacy of Os-based TLD1822 (320 μm) versus PpIX (10 mm ALA)

    Photodynamic therapy (PDT) efficacy of Os-based TLD1822 (320 μm) versus PpIX (10 mm ALA)

    Photodynamic therapy (PDT) efficacy of Os-based TLD1822 (320 μm) versus PpIX (10 mm ALA) in normoxic (A) and hypoxic (B) conditions under irradiation by red (625 nm, 90 J cm−2, 108 mW cm−2) light. Black bars denote dark toxicity (no light), and white bars denote PDT effect (total cell kill with light alone, and photosensitizer (PS) alone cell kills subtracted). The standard error of the mean is represented by error bars. The significance of cell kill being above zero was tested by one-tailed t-test: *P < 0.05, **P < 0.01.

  • The active sites of Anabaena sensory rhodopsin and of the sodium pump KR2

    The active sites of Anabaena sensory rhodopsin and of the sodium pump KR2

    The active sites of Anabaena sensory rhodopsin and of the sodium pump KR2 have extended hydrogen-bond networks. Selected amino acid residues and water oxygen atoms are shown as bonds and small spheres, respectively. (A) Close view of the retinal Schiff base region in the Anabaena sensory rhodopsin, where the active site has a hydrogen-bonded network including hydroxyl groups. (B) Close view of the extracellular side of KR2. Molecular graphics from panels A and B are based on crystal-structure PDB ID:1XIO, molecule B and PDB ID:3X3C , respectively. C&D Schematic representations of selected hydrogen bonds from structures represented in panels A and B, respectively.

  • ZnPT-induced mitochondrial redox dysregulation and stress response target gene expression

    ZnPT-induced mitochondrial redox dysregulation and stress response target gene expression

    ZnPT-induced mitochondrial redox dysregulation and stress response target gene expression in malignant HaCaT-ras II-4 keratinocytes. Cultured HaCaT-ras II-4 keratinocytes were exposed to ZnPT. (a) Time course of mitochondrial superoxide production in response to ZnPT (5 μm) as detected by MitoSOX Red™ fluorescence microscopy. Bar graph displays quantitative analysis of relative fluorescence intensity [scale bar: 200 μm (upper panels); 40 μm (overlay)]. (b) Mitochondrial transmembrane depolarization was assessed by JC-1 flow cytometric analysis 1 h after ZnPT (5 μm) treatment; numbers indicate percentage of cells inside the circle displaying intact Δψm [n = 3, mean ± SD; (P < 0.05)]. (c–d) Stress response gene expression in response to ZnPT exposure (1 μm; ≤6 h) was determined at the mRNA (fold induction versus ACTB; n = 3; mean ± SD) and protein levels using immunoblot analysis (loading control: β-actin).

  • Mechanistic proposal for the sodium salicylate-catalyzed PO-CL with TCPO and H2O2

    Mechanistic proposal for the sodium salicylate-catalyzed PO-CL with TCPO and H2O2

    Mechanistic proposal for the sodium salicylate-catalyzed PO-CL with TCPO and H2O2. Rate-limiting step: v = k1 [TCPO] [H2O2] [salicylate]. k1 = kter.

  • Phycoviolobilin (PVB) formation and spectral tuning in NpR6012g4 variants
  • Formation of reactive oxygen species (ROS) and subsequent cell killing
  • Absorption spectra
  • Photodynamic therapy (PDT) efficacy of Os-based TLD1822 (320 μm) versus PpIX (10 mm ALA)
  • The active sites of Anabaena sensory rhodopsin and of the sodium pump KR2
  • ZnPT-induced mitochondrial redox dysregulation and stress response target gene expression
  • Mechanistic proposal for the sodium salicylate-catalyzed PO-CL with TCPO and H2O2

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Synthetic DNA hairpins possessing a Michler’s ketone linker at one end separated from a perylenediimide base surrogate by four base pairs were designed to investigate transport of charge between the two chromophores. Pulsed laser excitation of Michler’s ketone with 400 nm light results in formation of a charge transfer excited state which returns to the group state without undergoing electron transport to perylenediimide (upper pathway). Excitation of perylenediimide with 545 nm light results in injection of a positive charge which is transported via the base pairs to Michler’s ketone (lower pathway). For details see the paper “Electronic Interactions of Michler’s Ketone with DNA Bases in Synthetic Hairpins” by Almaz S. Jalilov et al. on pages 739-747 in this issue.
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