Microbial Biotechnology

Cover image for Vol. 9 Issue 3

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Kenneth N. Timmis, Juan Luis Ramos, Willem de Vos, Siegfried E. Vlaeminck, and Auxiliadora Prieto

Impact Factor: 3.081

ISI Journal Citation Reports © Ranking: 2014: 39/119 (Microbiology); 48/163 (Biotechnology & Applied Microbiology)

Online ISSN: 1751-7915

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  1. Minireviews

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      Lactobacillus plantarum WCFS1 and its host interaction: a dozen years after the genome

      Maurits van den Nieuwboer, Saskia van Hemert, Eric Claassen and Willem M. de Vos

      Version of Record online: 27 MAY 2016 | DOI: 10.1111/1751-7915.12368

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      In this review, we present and discuss the main insight provided by the functional genomics research on L. plantarum WCFS1 with specific attention for the molecular mechanisms related to its interaction with the human host and its potential to modify the immune system, and induce other health-related benefits.

  2. Research Articles

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      Long-term ferrocyanide application via deicing salts promotes the establishment of Actinomycetales assimilating ferrocyanide-derived carbon in soil

      Silvia Gschwendtner, Tim Mansfeldt, Susanne Kublik, Evangelia Touliari, Franz Buegger and Michael Schloter

      Version of Record online: 19 MAY 2016 | DOI: 10.1111/1751-7915.12362

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      Deicing salt application leads to significant inputs of ferrocyanide into the road environment, having the potential to release highly toxic cyanide compounds. Despite its ecological relevance, data on its fate are missing so far. Our results revealed (1) that biodegradation in soil occured and (2) that bacteria using ferrocyanide as carbon source were highly similar in pre-exposed soils, belonging mostly to Actinomycetales, whereas in soils without pre-exposition bacteria belonging to Acidobacteria, Gemmatimonadetes and Gammaproteobacteria but not the present Actinomycetales assimilated ferrocyanide-derived carbon.

  3. Minireviews

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      Progress on lipid extraction from wet algal biomass for biodiesel production

      Forough Ghasemi Naghdi, Lina M González González, William Chan and Peer M Schenk

      Version of Record online: 19 MAY 2016 | DOI: 10.1111/1751-7915.12360

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      Lipid recovery and purification from microalgal cells is a significant bottleneck in microalgal oil production due to high costs involved and a high energy demand. The use of wet concentrated algal biomass as a feedstock for oil extraction is desirable as it avoids further concentration and/or drying. This article provides an overview on recent progress on lipid extraction from wet algal biomass and makes recommendations towards further improvements.

  4. Brief Reports

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      Underestimation of ammonia-oxidizing bacteria abundance by amplification bias in amoA-targeted qPCR

      Arnaud Dechesne, Sanin Musovic, Alejandro Palomo, Vaibhav Diwan and Barth F. Smets

      Version of Record online: 11 MAY 2016 | DOI: 10.1111/1751-7915.12366

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      In some sand filters treating groundwater for drinking water production, the abundance of ammonia-oxidizing bacteria (AOB) measured by a qPCR targeting the 16S rRNA gene was up to 50 times higher than that obtained with an amoA-based qPCR. We show, with an in silico primer analysis and cloning-sequencing, that the amoA primers underestimate AOB abundance when AOB Cluster 6A is abundant, while the 16S rRNA gene-qPCR is likely to be affected by amplification of non-target sequences.

  5. Research Articles

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      Secretion of small proteins is species-specific within Aspergillus sp

      Nicolas Valette, Isabelle Benoit-Gelber, Marcos Di Falco, Ad Wiebenga, Ronald P. de Vries, Eric Gelhaye and Mélanie Morel-Rouhier

      Version of Record online: 7 MAY 2016 | DOI: 10.1111/1751-7915.12361

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      In this analysis, we have compared the secretion pattern of small secreted proteins among 8 Aspergilli species in the context of plant biomass degradation and have highlighted putative interesting candidates that could be involved in the degradative process or in the strategies developed by fungi to resist the associated stress that could be due to the toxicity of some aromatic compounds or reactive oxygen species released during degradation.

  6. Minireviews

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      Whole cell biocatalysts: essential workers from Nature to the industry

      Carla C. C. R. de Carvalho

      Version of Record online: 3 MAY 2016 | DOI: 10.1111/1751-7915.12363

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      Rhodococcus erythropolis cells stained with Nile red.

  7. Opinion

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      Benefits and perspectives on the use of biofuels

      Juan-Luis Ramos, Miguel Valdivia, Francisco García-Lorente and Ana Segura

      Version of Record online: 26 APR 2016 | DOI: 10.1111/1751-7915.12356

      The current primary obstacle to biofuels is the current low price of fossil fuels, and the primary incentive to 2G biofuels is the growing world population and need to increase food suplies. Both of these will be increasingly subject to political, regulatory and legislative changes that will be positive for 2G biofuels.

  8. Minireviews

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      Lactic acid bacteria as a cell factory for riboflavin production

      Kiran Thakur, Sudhir Kumar Tomar and Sachinandan De

      Version of Record online: 21 DEC 2015 | DOI: 10.1111/1751-7915.12335

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      Fig 1. Advantages of microbes as cell factories for vitamin synthesis

  9. Brief Reports

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      EpsA is an essential gene in exopolysaccharide production in Lactobacillus johnsonii FI9785

      Enes Dertli, Melinda J. Mayer, Ian J. Colquhoun and Arjan Narbad

      Version of Record online: 24 SEP 2015 | DOI: 10.1111/1751-7915.12314

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      The epsA gene of Lactobacillus johnsonii FI9785 is essential for the production of both heteropolysaccharide EPS-2 and homopolysaccharide EPS-1. Overexpression of this gene can improve EPS production and affect EPS composition, EPS acetylation and surface characteristics of mutant strains.

  10. Research Articles

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      Accumulation of γ-aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy

      Haoyue Yang, Ronge Xing, Linfeng Hu, Song Liu and Pengcheng Li

      Version of Record online: 22 JUL 2015 | DOI: 10.1111/1751-7915.12301

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      A new strain of Enterococcus avium with high γ-aminobutyric acid (GABA)-producing ability from natural fermented scallop. It is the first time to accumulate GABA by E. avium. Scallop was first used as a culture medium to produce GABA.

    2. You have full text access to this OnlineOpen article
      Probiotic properties of Enterococcus strains isolated from traditional naturally fermented cream in China

      Lidong Guo, Tingting Li, Yaru Tang, Lijie Yang and Guicheng Huo

      Version of Record online: 22 JUL 2015 | DOI: 10.1111/1751-7915.12306

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      Enterococcus durans KLDS 6.0930 exhibited a excellent porbiotic properties, including acid and bile tolerance, adhesion ability, and cholesterol removal ability in media. There was a significant decrease in the total cholesterol and low-density lipoprotein cholesterol levels in the serum of Sprague-Dawley rats administrated with KLDS 6.0930 for 4 weeks, compared with the high-cholesterol diet control group. This strain may be used in the future as a good candidate for lowering human serum cholesterol levels.

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      Recombinant pediocin in Lactococcus lactis: increased production by propeptide fusion and improved potency by co-production with PedC

      Alexandre Back, Frédéric Borges, Cécile Mangavel, Cédric Paris, Emmanuel Rondags, Romain Kapel, Arnaud Aymes, Hélène Rogniaux, Marija Pavlović, Auke J. van Heel, Oscar P. Kuipers, Anne-Marie Revol-Junelles and Catherine Cailliez-Grimal

      Version of Record online: 3 JUL 2015 | DOI: 10.1111/1751-7915.12285

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      We show that heterologous production of pediocin in Lactococcus lactis can be improved both quantitatively and qualitatively. The amount of produced pediocin can be increased by inserting the propeptide SD or LEISSTCDA between the signal peptide and the structural mature bacteriocin. The potency of the bacteriocin can be improved by co-expressing the putative Thiol-Disulfide Oxidoreductase (TDOR) PedC.

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