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Microbial Biotechnology

All articles accepted from 30 October 2012 are published under the terms of the Creative Commons Attribution License.   Articles accepted before this date were published under the agreement as stated in the final article.

Cover image for Vol. 8 Issue 6

Edited By: Kenneth N. Timmis, Juan Luis Ramos, Willem de Vos, Siegfried E. Vlaeminck, and Auxiliadora Prieto

Impact Factor: 3.081

ISI Journal Citation Reports © Ranking: 2014: 39/119 (Microbiology); 48/163 (Biotechnology & Applied Microbiology)

Online ISSN: 1751-7915

Author Guidelines

Information for Contributors

Microbial Biotechnology invites original research articles related to the following subject areas:

• Biocatalysis and green chemistry
• Primary metabolites
• Food, beverages and supplements
• Secondary metabolites and natural products
• Pharmaceuticals
• Diagnostics
• Agricultural biotechnology and feedstock formation and conversions
• Algal biotechnology
• Bioenergy
• Biomining, including oil recovery and processing
• Bioremediation
• Biopolymers, biomaterials
• Bionanotechnology
• Biosurfactants and bioemulsifiers
• Compatible solutes and bioprotectants
• Biosensors, monitoring systems, quantitative microbial risk assessment
• Fungal biotechnology
• Technology development
• Protein engineering
• Functional genomics
• Metabolic design
• Systems analysis, modelling
• Process engineering
• Biologically-based analytical methods
• Microbially-based strategies in public health
• Microbially-based strategies to influence global processes

It is a sister journal of the premier microbiology journals Molecular Microbiology, Environmental Microbiology and Cellular Microbiology.

The types of articles published in the journal are:

• Research Articles
• Brief Reports
• Rapid Communications
• Reviews
• Minireviews
• Correspondence

In addition, "Microbial Genomics Update" and "Web Alert", plus other items relating to the journal content and biotechnological applicability, will be regularly featured.

Special Issues

Each year, one or two issues will be Special Issues devoted to particularly topical subjects, and edited by expert guest editors.

Submission of Manuscripts

Manuscripts must be submitted online at Full instructions and support are available on the site and a user ID and password can be obtained on the first visit. Support can be contacted by phone (+1 434 817 2040 ext. 167), e-mail ( or at Except in extenuating circumstances, only manuscripts submitted via the journal's online site will be considered for publication. If for some reason online submission is not possible please contact the Editorial Office for assistance.

Papers must be submitted exclusively to Microbial Biotechnology, and are accepted on the understanding that they have not been, and will not be, published elsewhere. The corresponding author must give consent to publication in a cover letter sent with the paper, which also should explicitly state that the content and authorship of the submitted manuscript has been approved by all authors.

Pre-submission English-language editing

Authors for whom English is a second language may choose to have their manuscript professionally edited before submission to improve the English. A list of independent suppliers of editing services can be found here. All services are paid for and arranged by the author, and use of one of these services does not guarantee acceptance or preference for publication.

Presentation of Manuscripts

Please note that authors should always retain a copy of all material. Papers should be as concise as possible, compatible with clarity and completeness, and should not normally exceed eight printed pages. As a guide, this corresponds to 17 pages of double line-spaced manuscript with three figures/tables, 15 pages of manuscript with six figures/tables or 14 pages of manuscript with eight figures/tables. The text should be double-spaced. Important material that would make the paper excessive in length, or that is not absolutely essential to document the main findings of the paper, may be submitted as "Supporting Information" (see below).

The title page should include the author's name(s), affiliation(s) where the work was conducted, and the address to which all correspondence and proofs should be sent. Telephone and fax numbers should also be supplied, along with an e-mail address, if available. Present addresses of authors should appear as a footnote. A running title of not more than 50 characters should be provided.

All original research papers must include a Summary not exceeding 200 words. The Summary should not include extensive background or describe what was done, but clearly formulate the principal findings and articulate the advance achieved. The main text of full papers should be subdivided into Introduction, Results, Discussion and Experimental Procedures, and can include additional subheadings. The Results and Discussion sections may be combined. Experimental procedures should be sufficiently detailed to enable the experiments to be reproduced.

In Brief Reports and Rapid Communications , Results and Discussion are combined, there is no section Experimental Procedures, and essential experimental details should be incorporated into the corresponding figure and table legends.

All pages must be numbered consecutively. Acknowledgments, References, Table and Figure legends should follow the main text, each starting on a new page. The preferred position of tables and figures should be indicated in the margin of the text. Footnotes should be avoided.


Files should be formatted double-spaced with no hyphenation and automatic word-wrap (no hard returns within paragraphs). Please type your text consistently, e.g. take care to distinguish between '1' (one) and 'l' (lower-case L) and '0' (zero) and 'O' (capital O), etc.


Authors should use the system illustrated below. Only full articles that have been published or are 'in press' may be included in the reference list. In the text, unpublished or submitted studies should be referred to as such (e.g. J. M. Smith, unpublished), or as a personal communication. It is the author's responsibility to obtain permission from colleagues to include their work as a personal communication. In the text, references should be inserted in parentheses in date order, as follows: (Pugsley, 1996; Matsunaga et al., 1997). The reference list should be in alphabetical order according to the first-named author. Papers with two authors should follow those of the first-named author, arranged in alphabetical order according to the name of the second author. Articles with more than two authors should follow those of the first named author in chronological order; with multiple references from the same first author in a given year, please list the references in cited order. The title of the article must be included. For papers with up to seven authors, the names of all authors should be listed. For papers with eight or more authors, the first six names should be listed, followed by 'et al.'. Standard abbreviations of journal titles should be used, as in the Index Medicus. Please see the following examples:

Straetz, M., Mau, M., and Timmis K.N. (1996) System to study horizontal gene exchange among microorganisms without cultivation of recipients. Mol Microbiol 22: 207-215.

Sahm, K., MacGregor, B.J., Jørgensen, B.B., and Stahl, D.A (1999) Sulphate reduction and vertical distribution of sulphate-reducing bacteria quantified by rRNA slot-blot hybridization in a coastal marine sediment. Environ Microbiol 1: 65-74.

Finlay, B.I., Fenchel, T., and Embley, T.M. (1993) Methanogen endosymbiosis in anaerobic climates. In Trends in Microbial Ecology. Guerrero, R., and Pedros-Alio, C. (eds). Barcelona: Spanish Society for Microbiology, pp. 285-288.

References to material available on the World Wide Web can be given, but only if the information is available without charge to readers on an official site. Authors will be asked to provide electronic copies of the cited material for inclusion on the Microbial Biotechnology web site at the discretion of the Editors. The format for citations is as follows:

Beckleheimer, J. (1994). How do you cite URLs in a bibliography? [WWW document]. URL


Tables should be typed as text, using 'tabs' to align columns. The use of table editors should be avoided. Do not use graphics software to create tables.


Please send us digital versions of your figures. The best quality of reproduction both in print and online can be achieved if files are saved in formats that are appropriate for the content of the image. More details are available on our Digital Illustration Page at EPS and TIFF files are preferable, but please consult our web site for further details. Please note that your paper will be processed more quickly if the figures are supplied according to these standards, and do not have to be relabelled or redrawn. Please ensure that electronic artwork is prepared such that, after reduction it will fit across one or two columns or two-thirds page width (80 mm, 169 mm or 110 mm, respectively) as required. Please also ensure that all lettering will be clear and easy to read at the final reproduction size, i.e. no labels should be too large or too small. Avoid using tints if possible; if they are essential to the understanding of the figure, try to make them coarse. No artwork should be incorporated into the text files.

In the full-text online edition of the journal, figure legends may be truncated in abbreviated links to the full-screen version. Therefore, the first 100 characters of any legend should inform the reader of key aspects of the figure.

Supporting Information

Supporting Information can be a useful way for an author to include important but ancillary information with the online version of an article. Examples of Supporting Information include additional tables, data sets, figures, movie files, audio clips, 3D structures, and other related nonessential multimedia files. Supporting Information should be cited within the article text, and a descriptive legend should be included. It is published as supplied by the author, and a proof is not made available prior to publication; for these reasons, authors should provide any Supporting Information in the desired final format. For further information on recommended file types and requirements for submission, please visit:

Genomics Data Submission

Any nucleotide sequence data reported or referred to in a submitted manuscript must be deposited in one of the three major collaborative databases-DDBJ/EMBL/GenBank-which exchange data on a daily basis. The suggested wording for referring to accession number information is: "These sequence data have been submitted to the DDBJ/EMBL/GenBank databases under accession number U12345". Micro-array experiments should be standardized (e.g. see:, and the data reported should be deposited in one of the principal public data bases, such as GEO ( and ArrayExpress ( and the corresponding accession number given.

Distribution of Strains

The publication of an article in Microbial Biotechnology is subject to the understanding that authors will distribute freely any strains, clones, antibodies or other reagents not readily available described therein, for use in academic research.


In-line equations should be typed as text. The use of graphics programs and aftermarket 'equation editors' should be avoided. Displayed equations are rekeyed by our typesetter.

Standard abbreviations should be as recommended in Quantities, Units, and Symbols (The Royal Society, 1988). Abbreviations of non-standard terms should follow, in parentheses, their first full usage.

External URLs

As web site content and addresses are constantly changing, we would prefer that authors, wherever possible, do not include external URLs in the main body of the manuscript. If possible please include necessary URLs as references as shown above.

Genetic Nomenclature

Standard genetic nomenclature should be used. For more detailed information, authors should consult Bachman (Microbiol Rev 47: 180-230, 1983) for E. coli K-12; Sanderson and Roth (Microbiol Rev 47: 310-453, 1983) for Salmonella typhimurium; Holloway et al. (Microbiol Rev 43: 73-102, 1979) for Bacillus subtilis; Perkins et al. (Microbiol Rev 46: 426-570, 1982) for Neurospora crassa; and the Handbook of Genetics Vol. 1 (R. C. King, ed., Plenum Press, 1974) for Saccharomyces cerevisiae.

Restriction Enzymes

Microbial Biotechnology has adopted the revised convention of naming restriction enzymes without italics. The previous style was EcoRI, KpnI, HindIII, SacII, etc. These should now be written EcoRI, KpnI, HindIII, SacII. For more information on the updated guidelines to naming restriction enzymes please consult Roberts et al. (Nucleic Acids Res 31: 1805-1812).

Accepted Manuscripts

Authors must ensure that all sections of their paper are submitted in editable formats. The main text, excluding figures, should be provided in a single Word or Rich Text Format file, updated to incorporate all revisions. Accepted manuscripts are transmitted by the Editor to the Production Office, which deals with all subsequent enquiries related to publication.

Word 2007

A document created in Word 2007 can result in compatibility problems with typesetting systems. Authors who are using Word 2007 to create their documents should follow the instructions to ensure their article is processed as efficiently: You should not use the 'Insert Equation'‚ feature on the Ribbon as this renders maths in a format that is unsuitable for typesetting. Instead, click Insert->Object->Microsoft Equation 3.0‚. After clicking on 'Object'‚ you will be shown a drop-down menu from which you must select 'Microsoft Equation 3.0'‚ to create your equation in the usual way.

Open Access Agreement

You will be prompted to sign the Open Access Agreement electronically after manuscript review and acceptance. We have introduced a convenient new process for signing your Open Access Agreement electronically (eOAA) that will save you considerable time and effort. If your paper is accepted, the author identified as the formal corresponding author for the paper will receive an email prompting them to login into Author Services, where via the Wiley Author Licensing Service (WALS) they will be able to complete the Creative Commons Attribution License OAA agreement on behalf of all authors on the paper. You may preview the copyright terms and conditions here.

Production Office:
Jonathan R. Rapay
Production Editor
SPi Bldg., Pascor Drive
Sto. Nino, Paranaque City 1700

Conflict of Interest

MBT requires that all authors disclose any potential sources of conflict of interest. Any interest or relationship, financial or otherwise, that might be perceived as influencing an author’s objectivity is considered a potential source of conflict of interest. These must be disclosed when directly relevant or indirectly related to the work that the authors describe in their manuscript. Potential sources of conflict of interest include but are not limited to patent or stock ownership, membership of a company board of directors, membership of an advisory board or committee for a company, and consultancy for or receipt of speaker’s fees from a company. The existence of a conflict of interest does not preclude publication in this journal.

If the authors have no conflict of interest to declare, they must also state this at submission.
It is the responsibility of the corresponding author to review this policy with all authors and to collectively list in the cover letter to the Editor, in the manuscript (under the Acknowledgment section), and in the online submission system ALL pertinent commercial and other relationships.

Referrals to the Open Access journal MicrobiologyOpen

This journal works together with Wiley’s open access journal, MicrobiologyOpen, to enable rapid publication of good quality research that is unable to be accepted for publication by our journal. Authors will be offered the option of having the paper, along with any related peer reviews, automatically transferred for consideration by the Editor of MicrobiologyOpen. Authors will not need to reformat or rewrite their manuscript at this stage, and publication decisions will be made a short time after the transfer takes place. The Editor of MicrobiologyOpen will accept submissions that report well-conducted research which reaches the standard acceptable for publication. The journal seeks to publish research, pure or applied, that furthers our understanding of microbial interactions and microbial processes. MicrobiologyOpen is compliant with open access mandates and will facilitate wide dissemination of your research findings, while continuing to uphold the Wiley tradition of publishing excellence. Accepted papers can be published rapidly, typically within 15 days of acceptance. MicrobiologyOpen is a Wiley open access journal and article publication fees apply. For more information, please go to

NEW: online production tracking is now available for your article through Wiley Blackwell's Author Services

Author Services enables authors to track their accepted article through the production process to publication online and in print. Authors can check the status of their articles online and choose to receive automated e-mails at key stages of production so they don't need to contact the production editor to check on progress. On receipt of the article at the publisher, the author will receive an e-mail with a unique link that enables them to register and have their article automatically added to the system. Please ensure that a complete e-mail address is provided when submitting the manuscript. Visit for more details on online production tracking and for a wealth of resources including FAQs and tips on article preparation, submission and more.


Page proofs will be despatched about four weeks after acceptance of papers and should be corrected and returned within two days of receipt. Proofs will be sent via email notification of a link with a downloadable Acrobat PDF (portable document format) file. Only corrections and essential changes can be made at this stage. The cost of any extensive changes will be charged to the authors. The journal reserves the right to make minor modifications to manuscripts that do not conform to accepted standards. Such alterations will always be submitted to the authors for approval at the proof stage. Online publication will normally be within 2 weeks of receipt of corrected proofs by the Production Office.

Early View

The journal is covered by Wiley Blackwell's Early View service. Early View articles are complete full-text articles published online in advance of their publication in a printed issue. Articles are therefore available as soon as they are ready, rather than having to wait for the next scheduled print issue. Early View articles are complete and final. They have been fully reviewed, revised and edited for publication, and the authors' final corrections have been incorporated. Because they are in final form, no changes can be made after online publication. The nature of Early View articles means that they do not yet have volume, issue or page numbers, so Early View articles cannot be cited in the traditional way. They are therefore given a Digital Object Identifier (DOI), which allows the article to be cited and tracked before it is allocated to an issue. After print publication, the DOI remains valid and can continue to be used to cite and access the article. More information about DOIs can be found at


Electronic offprints are sent to the corresponding author at his or her e-mail address on the title page of the paper, unless advised otherwise; therefore please ensure that the name, address and e-mail of the receiving author are clearly indicated on the manuscript title page. Free access to final PDF offprint of the published article will be provided to the corresponding author, and will be available via Wiley Blackwell Author Services only. Please register for free access by visiting Author Services and enjoy the many other benefits the service offers. PDF offprints may be distributed subject to the Publisher's terms and conditions.

Paper offprints may be purchased if ordered on the form sent with the proofs. Printed offprints are posted to the correspondence address given for the paper unless a different address is specified when ordered. Note that it is not uncommon for printed offprints to take up to eight weeks to arrive after publication of the journal.