Research Article

Proteomic analysis of a compatible interaction between sugarcane and Sporisorium scitamineum

  1. Leonard Barnabas1,
  2. N. M. R. Ashwin1,
  3. K. Kaverinathan1,
  4. Anna Rita Trentin2,
  5. Micaela Pivato2,
  6. A. Ramesh Sundar1,*,
  7. P. Malathi1,
  8. R. Viswanathan1,
  9. O. B. Rosana3,
  10. K. Neethukrishna3,
  11. Paolo Carletti2,
  12. Giorgio Arrigoni4,5,
  13. Antonio Masi2,
  14. Ganesh Kumar Agrawal6,7 and
  15. Randeep Rakwal6,7,8

Version of Record online: 4 APR 2016

DOI: 10.1002/pmic.201500245

Issue

PROTEOMICS

PROTEOMICS

Volume 16, Issue 7, pages 1111–1122, April 2016


Additional Information

How to Cite

Barnabas, L., Ashwin, N. M. R., Kaverinathan, K., Trentin, A. R., Pivato, M., Sundar, A. R., Malathi, P., Viswanathan, R., Rosana, O. B., Neethukrishna, K., Carletti, P., Arrigoni, G., Masi, A., Agrawal, G. K. and Rakwal, R. (2016), Proteomic analysis of a compatible interaction between sugarcane and Sporisorium scitamineum. Proteomics, 16: 1111–1122. doi: 10.1002/pmic.201500245

Author Information

  1. 1

    Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India

  2. 2

    Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy

  3. 3

    Bioinformatics Center, ICAR-Indian Institute of Spices Research, Kozhikode, India

  4. 4

    Proteomics Center of Padova University, Padova, Italy

  5. 5

    Department of Biomedical Sciences, University of Padova, Padova, Italy

  6. 6

    Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal

  7. 7

    GRADE (Global Research Arch for Developing Education) Academy Private Limited, Birgunj, Nepal

  8. 8

    Tsukuba International Academy for Sport Studies (TIAS) and Faculty of Health and Sport Sciences, University of Tsukuba, Ibaraki, Japan

*Correspondence: Dr. A. Ramesh Sundar, Plant Pathology Section, Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Coimbatore 641 007, Tamil Nadu, India
E-mail: rameshsundar_sbi@yahoo.co.in
Fax: +91-0422-2472923

  1. Colour Online: See the article online to view Figs. 2, 3 and 4 in colour.

Publication History

  1. Issue online: 4 APR 2016
  2. Version of Record online: 4 APR 2016
  3. Accepted manuscript online: 9 FEB 2016 03:54AM EST
  4. Manuscript Accepted: 2 FEB 2016
  5. Manuscript Revised: 5 JAN 2016
  6. Manuscript Received: 23 JUN 2015

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Enhanced Article (HTML) Get PDF (725K)Get PDF (725K)

Keywords:

  • Compatible interaction;
  • Smut;
  • Sporisorium scitamineum;
  • Sugarcane;
  • Plant proteomics

Smut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. scitamineum infected susceptible sugarcane cultivar at whip emergence stage. Differentially abundant proteins were identified by 2DE coupled with MALDI-TOF/TOF-MS. Comprehensively, 53 sugarcane proteins identified were related to defence, stress, metabolism, protein folding, energy, and cell division; in addition, a putative effector of S. scitamineum, chorismate mutase, was identified. Transcript expression vis-à-vis the activity of phenylalanine ammonia lyase was relatively higher in the infected meristem. Abundance of seven candidate proteins in 2D gel profiles was in correlation with its corresponding transcript expression levels as validated by qRT-PCR. Furthermore, this study has opened up new perspectives on the interaction between sugarcane and S. scitamineum.

View Full Article with Supporting Information (HTML) Enhanced Article (HTML) Get PDF (725K)Get PDF (725K)