Volume 101, Issue 10 p. 2915-2924
Original Article

Comparing biocompatibility of gingival fibroblasts and bacterial strains on a different modified titanium discs

Jana Franková,

Corresponding Author

Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic

Correspondence to: J. Franková; e-mail: frankova0@seznam.czSearch for more papers by this author
Veronika Pivodová,

Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic

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Filip Růžička,

Department of Microbiology, Faculty of Medicine, Masaryk University and St. Anne's University Hospital, Brno, Czech Republic

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Kateřina Tománková,

Department of Medical Biophysics, Faculty of Medicine and Dentistry, Institute of Translation Medicine, Palacký University Olomouc, Olomouc, Czech Republic

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Klára Šafářová,

Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic

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Jana Vrbková,

Department of Mathematical Analysis and Applications of Mathematics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic

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Jitka Ulrichová,

Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic

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First published: 25 March 2013
Citations: 13

Abstract

The modification of implant surface situated in the area of peri-implant sulcus has important role in bacterial and cell adhesion. Six different chemically and physically modified titanium discs were prepared: glazed (Tis-MALP), unglazed (Tis-O), unglazed and alkali-etched (Tis-OA), unglazed and coated with ZrN (Tis-OZ), unglazed, sand blasted, and acid etched (Tis-OPAE), and unglazed, sand blasted, acid, and alkali etched (Tis-OPAAE). Analysis of surface topography was determined using scanning electron microscopy and atomic force microscopy (AFM). Biocompatibility of gingival fibroblasts was characterized by the production of tumor necrosis factor alpha, collagen I, matrix metalloproteinase 2 (MMP-2) after 24 and 72 h and expression of α3β1 integrin and vinculin using enzyme-linked immunosorbent assay (ELISA) or modified ELISA after 6 and 24 h. Microorganism adhesion (five bacterial strains) and biofilm formation was also evaluated. The adhesion of bacteria and gingival fibroblasts was significantly higher on titanium disc Tis-OPAAE and biofilm formation on the same surface for Streptococcus mutans, Streptococcus gordonii, and Streptococcus intermedius. The gingival fibroblasts on Tis-OPAAE disc had also significantly lower production of MMP-2. The collagen production was significantly lower on all surfaces with roughness higher than 0.2 μm. This study confirmed that the titanium disc with the surface roughness 3.39 μm (Tis-OPAAE) supported the adhesion of bacterial strains as well as gingival fibroblasts. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A;2915–2924, 2013.

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