Communication
Synthesis of the Rheb and K‐Ras4B GTPases*
*
This research was supported by the Max‐Planck‐Gesellschaft, the Fonds der Chemischen Industrie, the DFG (SFB 642), and the Alexander von Humboldt‐Stiftung. We are grateful to Dr. C. Goemans and Prof. Dr. R. Heumann for providing DNA templates of Rheb, and C. Nowak for technical assistance.
Abstract
Now available! Farnesylated and carboxymethylated Rheb (see picture) and K‐Ras4B GTPases were synthesized in useful amounts by a combination of expressed protein ligation and solid‐phase lipopeptide synthesis. The functionality of the proteins was proven by biochemical, biophysical, and cell‐based investigations.
Citing Literature
Number of times cited: 40
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