Communication
Orthogonal Protein Decoration of DNA Origami*
*
This work was supported by the Deutsche Forschungsgemeinschaft (SA 1952/1‐1, NI 399/10‐1) and the Max‐Planck Society (fellowship to C.M.N.). We are most grateful to Prof. Kai Johnsson for the generous donation of the covalin expression plasmid and helpful discussions. We also thank Prof. Mathias Sprinzl for kind donation of a plasmid encoding for EST2, and Dr. Michael Adler for help with IPCR analyses. Stephanie Reisewitz and Julian Engel are acknowledged for experimental support in cloning.
Abstract
If the face fits: Self‐labeling fusion proteins have been used for the site‐specific decoration of DNA origami. This method even allows individual faces of the quasi‐two‐dimensional plane of the nanostructure to be specifically decorated (see picture), thereby enabling directional immobilization and thus control over the accessibility of distinct proteins presented on the structure.
Citing Literature
Number of times cited: 145
- Peiwen Wu, Yang Yu, Claire E. McGhee, Li H. Tan, Abhijit Mishra, Gerard Wong and Yi Lu, Applications of Synchrotron‐Based Spectroscopic Techniques in Studying Nucleic Acids and Nucleic‐Acid‐Based Nanomaterials, Synchrotron Radiation in Materials Science, (687-756), (2018).
- Yiyang Zhang, Dandan Bao, Shuo Wang, Yuancheng Dong, Fen Wu, Haitao Li and Dongsheng Liu, A Modularly Designable Vesicle for Sequentially Multiple Loading, Small, 14, 8, (2017).
- P Lourdu Xavier and Arun Richard Chandrasekaran, DNA-based construction at the nanoscale: emerging trends and applications, Nanotechnology, 10.1088/1361-6528/aaa120, 29, 6, (062001), (2018).
- Yu Tokura, Sean Harvey, Chaojian Chen, Yuzhou Wu, David Y. W. Ng and Tanja Weil, Fabrication of Defined Polydopamine Nanostructures by DNA Origami‐Templated Polymerization, Angewandte Chemie International Edition, 57, 6, (1587-1591), (2018).
- Yu Tokura, Sean Harvey, Chaojian Chen, Yuzhou Wu, David Y. W. Ng and Tanja Weil, Fabrication of Defined Polydopamine Nanostructures by DNA Origami‐Templated Polymerization, Angewandte Chemie, 130, 6, (1603-1607), (2018).
- Miran Liber, Toma E. Tomov, Roman Tsukanov, Yaron Berger, Mary Popov, Dinesh C. Khara and Eyal Nir, Study of DNA Origami Dimerization and Dimer Dissociation Dynamics and of the Factors that Limit Dimerization, Small, 14, 23, (2018).
- Alessandro Angelin, Olivier Kassel, Sepand Rastegar, Uwe Strähle and Christof M. Niemeyer, Protein‐Functionalized DNA Nanostructures as Tools to Control Transcription in Zebrafish Embryos, ChemistryOpen, 6, 1, (33-39), (2016).
- Isaac Gállego, Brendan Manning, Joan Daniel Prades, Mònica Mir, Josep Samitier and Ramon Eritja, DNA‐Origami‐Driven Lithography for Patterning on Gold Surfaces with Sub‐10 nm Resolution, Advanced Materials, 29, 11, (2017).
- M. Sajfutdinow, K. Uhlig, A. Prager, C. Schneider, B. Abel and D. M. Smith, Nanoscale patterning of self-assembled monolayer (SAM)-functionalised substrates with single molecule contact printing, Nanoscale, 10.1039/C7NR03696E, 9, 39, (15098-15106), (2017).
- Johanna Zessin, Franziska Fischer, Andreas Heerwig, Alfred Kick, Susanne Boye, Manfred Stamm, Anton Kiriy and Michael Mertig, Tunable Fluorescence of a Semiconducting Polythiophene Positioned on DNA Origami, Nano Letters, 10.1021/acs.nanolett.7b02623, 17, 8, (5163-5170), (2017).
- Thang Minh Nguyen, Eiji Nakata, Masayuki Saimura, Huyen Dinh and Takashi Morii, Design of Modular Protein Tags for Orthogonal Covalent Bond Formation at Specific DNA Sequences, Journal of the American Chemical Society, 10.1021/jacs.7b01640, 139, 25, (8487-8496), (2017).
- Heidi-Kristin Walter, Jens Bauer, Jeannine Steinmeyer, Akinori Kuzuya, Christof M. Niemeyer and Hans-Achim Wagenknecht, “DNA Origami Traffic Lights” with a Split Aptamer Sensor for a Bicolor Fluorescence Readout, Nano Letters, 17, 4, (2467), (2017).
- Yingning Gao, Samson Or, Aaron Toop and Ian Wheeldon, DNA Nanostructure Sequence-Dependent Binding of Organophosphates, Langmuir, 33, 8, (2033), (2017).
- Ehsan Akbari, Molly Y. Mollica, Christopher R. Lucas, Sarah M. Bushman, Randy A. Patton, Melika Shahhosseini, Jonathan W. Song and Carlos E. Castro, Engineering Cell Surface Function with DNA Origami, Advanced Materials, 29, 46, (2017).
- Chunxi Hou, Shuwen Guan, Ruidi Wang, Wei Zhang, Fanchao Meng, Linlu Zhao, Jiayun Xu and Junqiu Liu, Supramolecular Protein Assemblies Based on DNA Templates, The Journal of Physical Chemistry Letters, 10.1021/acs.jpclett.7b01564, 8, 17, (3970-3979), (2017).
- Mari Takahara, Rie Wakabayashi, Kosuke Minamihata, Masahiro Goto and Noriho Kamiya, Primary Amine-Clustered DNA Aptamer for DNA–Protein Conjugation Catalyzed by Microbial Transglutaminase, Bioconjugate Chemistry, 28, 12, (2954), (2017).
- Anja Henning-Knechtel, Johann Knechtel and Mazin Magzoub, DNA-assisted oligomerization of pore-forming toxin monomers into precisely-controlled protein channels, Nucleic Acids Research, 45, 21, (12057), (2017).
- Jerzy Kozyra, Alessandro Ceccarelli, Emanuela Torelli, Annunziata Lopiccolo, Jing-Ying Gu, Harold Fellermann, Ulrich Stimming and Natalio Krasnogor, Designing Uniquely Addressable Bio-orthogonal Synthetic Scaffolds for DNA and RNA Origami, ACS Synthetic Biology, 6, 7, (1140), (2017).
- Fan Hong, Fei Zhang, Yan Liu and Hao Yan, DNA Origami: Scaffolds for Creating Higher Order Structures, Chemical Reviews, 10.1021/acs.chemrev.6b00825, 117, 20, (12584-12640), (2017).
- Bas J. H. M. Rosier, Glenn A. O. Cremers, Wouter Engelen, Maarten Merkx, Luc Brunsveld and Tom F. A. de Greef, Incorporation of native antibodies and Fc-fusion proteins on DNA nanostructures via a modular conjugation strategy, Chemical Communications, 53, 53, (7393), (2017).
- Guido Grossi, Mette Dalgaard Ebbesen Jepsen, Jørgen Kjems and Ebbe Sloth Andersen, Control of enzyme reactions by a reconfigurable DNA nanovault, Nature Communications, 10.1038/s41467-017-01072-8, 8, 1, (2017).
- Arun Richard Chandrasekaran, Nate Anderson, Megan Kizer, Ken Halvorsen and Xing Wang, Beyond the Fold: Emerging Biological Applications of DNA Origami, ChemBioChem, 17, 12, (1081-1089), (2016).
- Julián Valero, Finn Lohmann, Daniel Keppner and Michael Famulok, Single‐Stranded Tile Stoppers for Interlocked DNA Architectures, ChemBioChem, 17, 12, (1146-1149), (2016).
- Zai‐Sheng Wu, Zhifa Shen, Kha Tram, Bruno J. Salena and Yingfu Li, Topological DNA Assemblies Containing Identical or Fraternal Twins, ChemBioChem, 17, 12, (1142-1145), (2016).
- Katja J. Koßmann, Cornelia Ziegler, Alessandro Angelin, Rebecca Meyer, Marc Skoupi, Kersten S. Rabe and Christof M. Niemeyer, A Rationally Designed Connector for Assembly of Protein‐Functionalized DNA Nanostructures, ChemBioChem, 17, 12, (1102-1106), (2016).
- Yu Tokura, Yanyan Jiang, Alexander Welle, Martina H. Stenzel, Katarzyna M. Krzemien, Jens Michaelis, Rüdiger Berger, Christopher Barner‐Kowollik, Yuzhou Wu and Tanja Weil, Bottom‐Up Fabrication of Nanopatterned Polymers on DNA Origami by In Situ Atom‐Transfer Radical Polymerization, Angewandte Chemie, 128, 19, (5786-5791), (2016).
- R. F. Sommese, R. F. Hariadi, K. Kim, M. Liu, M. J. Tyska and S. Sivaramakrishnan, Patterning protein complexes on DNA nanostructures using a GFP nanobody, Protein Science, 25, 11, (2089-2094), (2016).
- Roland Hager, Jonathan R. Burns, Martyna J. Grydlik, Alma Halilovic, Thomas Haselgrübler, Friedrich Schäffler and Stefan Howorka, Co‐Immobilization of Proteins and DNA Origami Nanoplates to Produce High‐Contrast Biomolecular Nanoarrays, Small, 12, 21, (2877-2884), (2016).
- Magdiel Inggrid Setyawati, Rajaletchumy Veloo Kutty and David Tai Leong, DNA Nanostructures Carrying Stoichiometrically Definable Antibodies, Small, 12, 40, (5601-5611), (2016).
- Patrick D. Halley, Christopher R. Lucas, Emily M. McWilliams, Matthew J. Webber, Randy A. Patton, Comert. Kural, David M. Lucas, John C. Byrd and Carlos E. Castro, Daunorubicin‐Loaded DNA Origami Nanostructures Circumvent Drug‐Resistance Mechanisms in a Leukemia Model, Small, 12, 3, (308-320), (2015).
- Yu Tokura, Yanyan Jiang, Alexander Welle, Martina H. Stenzel, Katarzyna M. Krzemien, Jens Michaelis, Rüdiger Berger, Christopher Barner‐Kowollik, Yuzhou Wu and Tanja Weil, Bottom‐Up Fabrication of Nanopatterned Polymers on DNA Origami by In Situ Atom‐Transfer Radical Polymerization, Angewandte Chemie International Edition, 55, 19, (5692-5697), (2016).
- Ziwen Dai, Qi Gao, Man Ching Cheung, Hoi Man Leung, Terrence Chi Kong Lau, Hanadi F. Sleiman, King Wai Chiu Lai and Pik Kwan Lo, A highly versatile platform based on geometrically well-defined 3D DNA nanostructures for selective recognition and positioning of multiplex targets, Nanoscale, 10.1039/C6NR05411K, 8, 43, (18291-18295), (2016).
- Jenny V. Le, Yi Luo, Michael A. Darcy, Christopher R. Lucas, Michelle F. Goodwin, Michael G. Poirier and Carlos E. Castro, Probing Nucleosome Stability with a DNA Origami Nanocaliper, ACS Nano, 10, 7, (7073), (2016).
- Ruokun Jia, Ting Wang, Qiao Jiang, Zhengang Wang, Chen Song and Baoquan Ding, Self‐Assembled DNA Nanostructures for Drug Delivery, Chinese Journal of Chemistry, 34, 3, (265-272), (2016).
- Hisashi Tadakuma, Takeya Masubuchi and Takuya Ueda, RNA Study Using DNA Nanotechnology, Nanotechnology Tools for the Study of RNA, 10.1016/bs.pmbts.2015.11.004, (121-163), (2016).
- Piero Morales, Liqian Wang, Abhichart Krissanaprasit, Claudia Dalmastri, Mario Caruso, Mattia De Stefano, Lucia Mosiello, Bruno Rapone, Antonio Rinaldi, Stefano Vespucci, Jesper Vinther, Scott Retterer and Kurt V. Gothelf, Suspending DNA Origami Between Four Gold Nanodots, Small, 12, 2, (169-173), (2015).
- Saminathan Ramakrishnan, Sivaraman Subramaniam, A. Francis Stewart, Guido Grundmeier and Adrian Keller, Regular Nanoscale Protein Patterns via Directed Adsorption through Self-Assembled DNA Origami Masks, ACS Applied Materials & Interfaces, 8, 45, (31239), (2016).
- Xin Li, Yanbo Li, Li Hong and Jean-Luc EPH Darlix, A Novel Self-Assembling DNA Nano Chip for Rapid Detection of Human Papillomavirus Genes, PLOS ONE, 11, 10, (e0162975), (2016).
- Weina Fang, Jianbang Wang and Huajie Liu, Smart Materials for DNA-Based Nanoconstructions, Design, Fabrication, Properties and Applications of Smart and Advanced Materials, 10.1201/b19977-3, (13-52), (2016).
- Yinan Zhang, Jie Chao, Huajie Liu, Fei Wang, Shao Su, Bing Liu, Lan Zhang, Jiye Shi, Lihua Wang, Wei Huang, Lianhui Wang and Chunhai Fan, Transfer of Two‐Dimensional Oligonucleotide Patterns onto Stereocontrolled Plasmonic Nanostructures through DNA‐Origami‐Based Nanoimprinting Lithography, Angewandte Chemie International Edition, 55, 28, (8036-8040), (2016).
- Yinan Zhang, Jie Chao, Huajie Liu, Fei Wang, Shao Su, Bing Liu, Lan Zhang, Jiye Shi, Lihua Wang, Wei Huang, Lianhui Wang and Chunhai Fan, Transfer of Two‐Dimensional Oligonucleotide Patterns onto Stereocontrolled Plasmonic Nanostructures through DNA‐Origami‐Based Nanoimprinting Lithography, Angewandte Chemie, 128, 28, (8168-8172), (2016).
- Sandra Sagredo, Tobias Pirzer, Ali Aghebat Rafat, Marisa A. Goetzfried, Gabriel Moncalian, Friedrich C. Simmel and Fernando de la Cruz, , Angewandte Chemie, 128, 13, (4421-4425), (2016).
- Sandra Sagredo, Tobias Pirzer, Ali Aghebat Rafat, Marisa A. Goetzfried, Gabriel Moncalian, Friedrich C. Simmel and Fernando de la Cruz, Orthogonal Protein Assembly on DNA Nanostructures Using Relaxases, Angewandte Chemie International Edition, 55, 13, (4348-4352), (2016).
- Chun‐Hua Lu and Itamar Willner, Stimuliresponsive DNA‐funktionalisierte Nano‐ und Mikrocontainer zur schaltbaren und kontrollierten Freisetzung, Angewandte Chemie, 127, 42, (12380-12405), (2015).
- Christopher Timm and Christof M. Niemeyer, Assembly and Purification of Enzyme‐Functionalized DNA Origami Structures, Angewandte Chemie International Edition, 54, 23, (6745-6750), (2015).
- Christopher Timm and Christof M. Niemeyer, Assembly and Purification of Enzyme‐Functionalized DNA Origami Structures, Angewandte Chemie, 127, 23, (6849-6854), (2015).
- Chun‐Hua Lu and Itamar Willner, Stimuli‐Responsive DNA‐Functionalized Nano‐/Microcontainers for Switchable and Controlled Release, Angewandte Chemie International Edition, 54, 42, (12212-12235), (2015).
- Marc Skoupi, Carine Vaxelaire, Carsten Strohmann, Mathias Christmann and Christof M. Niemeyer, Enantiogroup‐Differentiating Biocatalytic Reductions of Prochiral Cs‐Symmetrical Dicarbonyl Compounds to meso Compounds, Chemistry – A European Journal, 21, 24, (8701-8705), (2015).
- Miran Liber, Toma E. Tomov, Roman Tsukanov, Yaron Berger and Eyal Nir, A Bipedal DNA Motor that Travels Back and Forth between Two DNA Origami Tiles, Small, 11, 5, (568-575), (2014).
- Juan Elezgaray, Jean-Pierre Aime and Jean-Michel Arbona, DNA-Origamis as Protein Nanocarriers, Enzyme Nanocarriers, 10.1201/b18970-7, (193-206), (2015).
- Lifeng Zhou, Alexander E. Marras, Hai-Jun Su and Carlos E. Castro, Direct Design of an Energy Landscape with Bistable DNA Origami Mechanisms, Nano Letters, 10.1021/nl5045633, 15, 3, (1815-1821), (2015).
- Hosna Jabbari, Maral Aminpour and Carlo Montemagno, Computational Approaches to Nucleic Acid Origami, ACS Combinatorial Science, 10.1021/acscombsci.5b00079, 17, 10, (535-547), (2015).
- Cuiping Ma, Zhiwei Wu, Wenshuo Wang, Qianqian Jiang and Chao Shi, Three-dimensional DNA nanostructures for colorimetric assay of nucleic acids, J. Mater. Chem. B, 3, 14, (2853), (2015).
- Hao Qi, Guoyou Huang, Yulong Han, Xiaohui Zhang, Yuhui Li, Belinda Pingguan-Murphy, Tian Jian Lu, Feng Xu and Lin Wang, Engineering Artificial Machines from Designable DNA Materials for Biomedical Applications, Tissue Engineering Part B: Reviews, 10.1089/ten.teb.2014.0494, 21, 3, (288-297), (2015).
- L. Wang and G. Arrabito, Hybrid, multiplexed, functional DNA nanotechnology for bioanalysis, The Analyst, 10.1039/C5AN00861A, 140, 17, (5821-5848), (2015).
- , Poster Presentations, Regenerative Medicine, 10, 7s, (S96), (2015).
- Yuhe R. Yang, Yan Liu and Hao Yan, DNA Nanostructures as Programmable Biomolecular Scaffolds, Bioconjugate Chemistry, 10.1021/acs.bioconjchem.5b00194, 26, 8, (1381-1395), (2015). TRANSDUCERS 2015 - 2015 18th International Solid-State Sensors, Actuators and Microsystems Conference Anchorage, AK, USA 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) IEEE , (2015). 978-1-4799-8955-3 Y. Mori, Z. Ma, S. Park, Y. Hirai, T. Tsuchiya and O. Tabata Selective assembly of DNA nanostructure bridging onto a trenched silicon substrate , (2015). 1389 1392 7181192 , 10.1109/TRANSDUCERS.2015.7181192 http://ieeexplore.ieee.org/document/7181192/
- Jiang Li, Fan Li, Hao Pei, Lihua Wang, Qing Huang and Chunhai Fan, Construction of Functional DNA Nanostructures for Theranostic Applications, Advanced Theranostic Materials, (93-130), (2015).
- Alexander E. Marras, Lifeng Zhou, Hai-Jun Su and Carlos E. Castro, Programmable motion of DNA origami mechanisms, Proceedings of the National Academy of Sciences, 10.1073/pnas.1408869112, 112, 3, (713-718), (2015).
- Maria D.R. Peralta, Arpad Karsai, Alice Ngo, Catherine Sierra, Kai T. Fong, Natha Robert Hayre, Nima Mirzaee, Krishnakumar Mayuram Ravikumar, Alexander J. Kluber, Xi Chen, Gang-yu Liu, Michael D. Toney, Rajiv. R. Singh and Daniel Lee Cox, Engineering Amyloid Fibrils from β-Solenoid Proteins for Biomaterials Applications, ACS Nano, 9, 1, (449), (2015).
- Carlos E. Castro, Hai-Jun Su, Alexander E. Marras, Lifeng Zhou and Joshua Johnson, Mechanical design of DNA nanostructures, Nanoscale, 10.1039/C4NR07153K, 7, 14, (5913-5921), (2015).
- E. P. Gates, J. K. Jensen, J. N. Harb and A. T. Woolley, Optimizing gold nanoparticle seeding density on DNA origami, RSC Advances, 10.1039/C4RA15451G, 5, 11, (8134-8141), (2015).
- Henna Päkkilä, Riikka Peltomaa, Urpo Lamminmäki and Tero Soukka, Precise construction of oligonucleotide–Fab fragment conjugate for homogeneous immunoassay using HaloTag technology, Analytical Biochemistry, 472, (37), (2015).
- Théo Calais, Benoit Playe, Jean-Marie Ducéré, Jean-François Veyan, Sara Rupich, Anne Hemeryck, Mehdi Djafari Rouhani, Carole Rossi, Yves J. Chabal and Alain Estève, Role of Alumina Coatings for Selective and Controlled Bonding of DNA on Technologically Relevant Oxide Surfaces, The Journal of Physical Chemistry C, 119, 41, (23527), (2015).
- Eiji Nakata, Huyen Dinh, Tien Anh Ngo, Masayuki Saimura and Takashi Morii, A modular zinc finger adaptor accelerates the covalent linkage of proteins at specific locations on DNA nanoscaffolds, Chemical Communications, 51, 6, (1016), (2015).
- Peiwen Wu, Yang Yu, Claire E. McGhee, Li Huey Tan and Yi Lu, Applications of Synchrotron‐Based Spectroscopic Techniques in Studying Nucleic Acids and Nucleic Acid‐Functionalized Nanomaterials, Advanced Materials, 26, 46, (7849-7872), (2014).
- Maria Tintoré, Ramon Eritja and Carmen Fábrega, DNA Nanoarchitectures: Steps towards Biological Applications, ChemBioChem, 15, 10, (1374-1390), (2014).
- Fei Zhang, Jeanette Nangreave, Yan Liu and Hao Yan, Structural DNA Nanotechnology: State of the Art and Future Perspective, Journal of the American Chemical Society, 10.1021/ja505101a, 136, 32, (11198-11211), (2014).
- Zhen-Gang Wang and Baoquan Ding, Engineering DNA Self-Assemblies as Templates for Functional Nanostructures, Accounts of Chemical Research, 47, 6, (1654), (2014).
- Stefan Howorka and Jan Hesse, Microarrays and single molecules: an exciting combination, Soft Matter, 10.1039/c3sm52561a, 10, 7, (931), (2014).
- Zhong Jin, Wei Sun, Peng Yin and Michael S. Strano, Nanolithography Based on Metalized DNA Templates for Graphene Patterning, Current Protocols in Chemical Biology, 6, 2, (53-64), (2014).
- Rebecca Meyer, Stefan Giselbrecht, Bastian E Rapp, Michael Hirtz and Christof M Niemeyer, Advances in DNA-directed immobilization, Current Opinion in Chemical Biology, 10.1016/j.cbpa.2013.10.023, 18, (8-15), (2014).
- Lars Opherden, Jana Oertel, Astrid Barkleit, Karim Fahmy and Adrian Keller, Paramagnetic Decoration of DNA Origami Nanostructures by Eu 3+ Coordination , Langmuir, 10.1021/la501112a, 30, 27, (8152-8159), (2014).
- Jonathan R. Burns, Noura Al‐Juffali, Sam M. Janes and Stefan Howorka, Back Cover: Membrane‐Spanning DNA Nanopores with Cytotoxic Effect (Angew. Chem. Int. Ed. 46/2014), Angewandte Chemie International Edition, 53, 46, (12644-12644), (2014).
- Yu-Chueh Hung, Dennis M. Bauer, Ishtiaq Ahmed and Ljiljana Fruk, DNA from natural sources in design of functional devices, Methods, 10.1016/j.ymeth.2014.03.003, 67, 2, (105-115), (2014).
- Ali Aghebat Rafat, Tobias Pirzer, Max B. Scheible, Anna Kostina and Friedrich C. Simmel, Surface‐Assisted Large‐Scale Ordering of DNA Origami Tiles, Angewandte Chemie International Edition, 53, 29, (7665-7668), (2014).
- Samar Najjar, David Talaga, Léonard Schué, Yannick Coffinier, Sabine Szunerits, Rabah Boukherroub, Laurent Servant, Vincent Rodriguez and Sébastien Bonhommeau, Tip-Enhanced Raman Spectroscopy of Combed Double-Stranded DNA Bundles, The Journal of Physical Chemistry C, 118, 2, (1174), (2014).
- Ali Aghebat Rafat, Tobias Pirzer, Max B. Scheible, Anna Kostina and Friedrich C. Simmel, Oberflächenunterstützte großflächige Anordnung von DNA‐Origami‐Kacheln, Angewandte Chemie, 126, 29, (7797-7801), (2014).
- Tien Anh Ngo, Eiji Nakata, Masayuki Saimura, Tsutomu Kodaki and Takashi Morii, A protein adaptor to locate a functional protein dimer on molecular switchboard, Methods, 10.1016/j.ymeth.2013.10.014, 67, 2, (142-150), (2014).
- Takahiro Yamazaki, Jonathan Gardiner Heddle, Akinori Kuzuya and Makoto Komiyama, Orthogonal enzyme arrays on a DNA origami scaffold bearing size-tunable wells, Nanoscale, 6, 15, (9122), (2014).
- Lynn M. McGregor, Tara Jain and David R. Liu, Identification of Ligand–Target Pairs from Combined Libraries of Small Molecules and Unpurified Protein Targets in Cell Lysates, Journal of the American Chemical Society, 136, 8, (3264), (2014).
- Justin D. Flory, Chad R. Simmons, Su Lin, Trey Johnson, Alessio Andreoni, James Zook, Giovanna Ghirlanda, Yan Liu, Hao Yan and Petra Fromme, Low Temperature Assembly of Functional 3D DNA-PNA-Protein Complexes, Journal of the American Chemical Society, 136, 23, (8283), (2014).
- Max B. Scheible, Günther Pardatscher, Anton Kuzyk and Friedrich C. Simmel, Single Molecule Characterization of DNA Binding and Strand Displacement Reactions on Lithographic DNA Origami Microarrays, Nano Letters, 14, 3, (1627), (2014).
- M. Su, María Tomás-Gamasa, S. Serdjukow, P. Mayer and T. Carell, Synthesis and properties of a Cu( ii ) complexing pyrazole ligandoside in DNA , Chem. Commun., 10.1039/C3CC47561A, 50, 4, (409-411), (2014).
- Michael Fairhead, Denis Krndija, Ed D. Lowe and Mark Howarth, Plug-and-Play Pairing via Defined Divalent Streptavidins, Journal of Molecular Biology, 10.1016/j.jmb.2013.09.016, 426, 1, (199-214), (2014).
- Jonathan R. Burns, Noura Al‐Juffali, Sam M. Janes and Stefan Howorka, Membrane‐Spanning DNA Nanopores with Cytotoxic Effect, Angewandte Chemie, 126, 46, (12674-12678), (2014).
- Elisabeth P. Gates, Andrew M. Dearden and Adam T. Woolley, DNA‐templated lithography and nanofabrication for the fabrication of nanoscale electronic circuitry, Critical Reviews in Analytical Chemistry, 10.1080/10408347.2014.910636, 44, 4, (354-370), (2014).
- Jiang Li, Chunhai Fan, Hao Pei, Jiye Shi and Qing Huang, Smart Drug Delivery Nanocarriers with Self‐Assembled DNA Nanostructures, Advanced Materials, 25, 32, (4386-4396), (2013).
- Zhen‐Gang Wang, Chen Song and Baoquan Ding, Functional DNA Nanostructures for Photonic and Biomedical Applications, Small, 9, 13, (2210-2222), (2013).
- Jonathan R. Burns, Kerstin Göpfrich, James W. Wood, Vivek V. Thacker, Eugen Stulz, Ulrich F. Keyser and Stefan Howorka, Lipid‐Bilayer‐Spanning DNA Nanopores with a Bifunctional Porphyrin Anchor, Angewandte Chemie, 125, 46, (12291-12294), (2013).
- Suchetan Pal, Palash Dutta, Haining Wang, Zhengtao Deng, Shengli Zou, Hao Yan and Yan Liu, Quantum Efficiency Modification of Organic Fluorophores Using Gold Nanoparticles on DNA Origami Scaffolds, The Journal of Physical Chemistry C, 117, 24, (12735), (2013).
- Maria Tintoré, Isaac Gállego, Brendan Manning, Ramon Eritja and Carme Fàbrega, DNA Origami as a DNA Repair Nanosensor at the Single‐Molecule Level, Angewandte Chemie, 125, 30, (7901-7904), (2013).
- Lianqing Liu, Wen J. Li, Longhai Li, Osamu Tabata, Zaili Dong and Xiaojun Tian, Manipulation of DNA origami nanotubes in liquid using programmable tapping-mode atomic force microscopy, Micro & Nano Letters, 8, 10, (641), (2013).
- Hiroshi Atsumi, Shigeaki Nakazawa, Chikara Dohno, Kazunobu Sato, Takeji Takui and Kazuhiko Nakatani, Ligand-induced electron spin-assembly on a DNA tile, Chemical Communications, 10.1039/c3cc41801d, 49, 57, (6370), (2013).
- David Smith, Verena Schüller, Christian Engst, Joachim Rädler and Tim Liedl, Nucleic acid nanostructures for biomedical applications, Nanomedicine, 8, 1, (105), (2013).
- Jyun-Liang Lin and Ian Wheeldon, Kinetic Enhancements in DNA–Enzyme Nanostructures Mimic the Sabatier Principle, ACS Catalysis, 3, 4, (560), (2013).
- Rasmus Schøler Sørensen, Anders Hauge Okholm, David Schaffert, Anne Louise Bank Kodal, Kurt V. Gothelf and Jørgen Kjems, Enzymatic Ligation of Large Biomolecules to DNA, ACS Nano, 7, 9, (8098), (2013).
- Thua N. N. Tran, Jinhui Cui, Mark R. Hartman, Songming Peng, Hisakage Funabashi, Faping Duan, Dayong Yang, John C. March, John T. Lis, Haixin Cui and Dan Luo, A Universal DNA-Based Protein Detection System, Journal of the American Chemical Society, 10.1021/ja405872g, 135, 38, (14008-14011), (2013).
- Dennis M. Bauer, Ishtiaq Ahmed, Antonina Vigovskaya and Ljiljana Fruk, Clickable Tyrosine Binding Bifunctional Linkers for Preparation of DNA–Protein Conjugates, Bioconjugate Chemistry, 10.1021/bc4001799, 24, 6, (1094-1101), (2013).
- Roman Tsukanov, Toma E. Tomov, Rula Masoud, Hagai Drory, Noa Plavner, Miran Liber and Eyal Nir, Detailed Study of DNA Hairpin Dynamics Using Single-Molecule Fluorescence Assisted by DNA Origami, The Journal of Physical Chemistry B, 117, 40, (11932), (2013).
- Jonathan R. Burns, Kerstin Göpfrich, James W. Wood, Vivek V. Thacker, Eugen Stulz, Ulrich F. Keyser and Stefan Howorka, Lipid‐Bilayer‐Spanning DNA Nanopores with a Bifunctional Porphyrin Anchor, Angewandte Chemie International Edition, 52, 46, (12069-12072), (2013).
- Michael P. Coyle, Qian Xu, Samantha Chiang, Matthew B. Francis and Jay T. Groves, DNA-Mediated Assembly of Protein Heterodimers on Membrane Surfaces, Journal of the American Chemical Society, 10.1021/ja3101215, 135, 13, (5012-5016), (2013).
- Jie Zhu, Xiaolu Feng, Jingyu Lou, Weidong Li, Sheng Li, Hongxin Zhu, Lun Yang, Aiping Zhang, Lin He, Can Li and Meni Wanunu, Accurate Quantification of microRNA via Single Strand Displacement Reaction on DNA Origami Motif, PLoS ONE, 8, 8, (e69856), (2013).
- Masayuki Endo, Yangyang Yang and Hiroshi Sugiyama, DNA origami technology for biomaterials applications, Biomater. Sci., 1, 4, (347), (2013).
- Maria Tintoré, Isaac Gállego, Brendan Manning, Ramon Eritja and Carme Fàbrega, DNA Origami as a DNA Repair Nanosensor at the Single‐Molecule Level, Angewandte Chemie International Edition, 52, 30, (7747-7750), (2013).
- David Smith, Programmed nucleic acid assembly for nanomedicines, Nucleic Acid-Based Drugs, 10.4155/ebo.13.438, (116-131), (2013).
- Jürgen J. Schmied, Carsten Forthmann, Enrico Pibiri, Birka Lalkens, Philipp Nickels, Tim Liedl and Philip Tinnefeld, DNA Origami Nanopillars as Standards for Three-Dimensional Superresolution Microscopy, Nano Letters, 13, 2, (781), (2013).
- Na Wu, Daniel M. Czajkowsky, Jinjin Zhang, Jianxun Qu, Ming Ye, Dongdong Zeng, Xingfei Zhou, Jun Hu, Zhifeng Shao, Bin Li and Chunhai Fan, Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures, Journal of the American Chemical Society, 10.1021/ja403863a, 135, 33, (12172-12175), (2013).
- Stefan Howorka, DNA Nanoarchitectonics: Assembled DNA at Interfaces, Langmuir, 10.1021/la3045785, 29, 24, (7344-7353), (2013).
- Karl Börjesson, Jakob G. Woller, Elham Parsa, Jerker Mårtensson and Bo Albinsson, A bioinspired self assembled dimeric porphyrin pocket that binds electron accepting ligands, Chemical Communications, 48, 12, (1793), (2012).
- Barbara Saccà and Christof M. Niemeyer, DNA‐Origami: die Kunst, DNA zu falten, Angewandte Chemie, 124, 1, (60-69), (2011).
- Eiji Nakata, Fong Fong Liew, Chisana Uwatoko, Shigeki Kiyonaka, Yasuo Mori, Yousuke Katsuda, Masayuki Endo, Hiroshi Sugiyama and Takashi Morii, Zinc‐Finger Proteins for Site‐Specific Protein Positioning on DNA‐Origami Structures, Angewandte Chemie International Edition, 51, 10, (2421-2424), (2012).
- Eiji Nakata, Fong Fong Liew, Chisana Uwatoko, Shigeki Kiyonaka, Yasuo Mori, Yousuke Katsuda, Masayuki Endo, Hiroshi Sugiyama and Takashi Morii, Zinc‐Finger Proteins for Site‐Specific Protein Positioning on DNA‐Origami Structures, Angewandte Chemie, 124, 10, (2471-2474), (2012).
- Josui Shimada, Tatsuo Maruyama, Momoko Kitaoka, Hisao Yoshinaga, Koji Nakano, Noriho Kamiya and Masahiro Goto, Programmable protein–protein conjugation via DNA-based self-assembly, Chemical Communications, 10.1039/c2cc30618b, 48, 50, (6226), (2012).
- Yangyang Yang, Masayuki Endo, Kumi Hidaka and Hiroshi Sugiyama, Photo-Controllable DNA Origami Nanostructures Assembling into Predesigned Multiorientational Patterns, Journal of the American Chemical Society, 134, 51, (20645), (2012).
- Hidehiko Hirakawa, Tomoaki Haga and Teruyuki Nagamune, Artificial Protein Complexes for Biocatalysis, Topics in Catalysis, 10.1007/s11244-012-9900-5, 55, 16-18, (1124-1137), (2012).
- Jinglin Fu, Minghui Liu, Yan Liu and Hao Yan, Spatially-Interactive Biomolecular Networks Organized by Nucleic Acid Nanostructures, Accounts of Chemical Research, 10.1021/ar200295q, 45, 8, (1215-1226), (2012).
- Ofer I. Wilner and Itamar Willner, Functionalized DNA Nanostructures, Chemical Reviews, 10.1021/cr200104q, 112, 4, (2528-2556), (2012).
- Daniela Cardinale, Noëlle Carette and Thierry Michon, Virus scaffolds as enzyme nano-carriers, Trends in Biotechnology, 30, 7, (369), (2012).
- Jinglin Fu, Minghui Liu, Yan Liu, Neal W. Woodbury and Hao Yan, Interenzyme Substrate Diffusion for an Enzyme Cascade Organized on Spatially Addressable DNA Nanostructures, Journal of the American Chemical Society, 10.1021/ja300897h, 134, 12, (5516-5519), (2012).
- Dana A. Uhlenheuer, Dorothee Wasserberg, Christian Haase, Hoang D. Nguyen, Jan Hendrik Schenkel, Jurriaan Huskens, Bart Jan Ravoo, Pascal Jonkheijm and Luc Brunsveld, Directed Supramolecular Surface Assembly of SNAP‐tag Fusion Proteins, Chemistry – A European Journal, 18, 22, (6788-6794), (2012).
- Barbara Saccà and Christof M. Niemeyer, DNA Origami: The Art of Folding DNA, Angewandte Chemie International Edition, 51, 1, (58-66), (2011).
- Rebecca Meyer and Christof M. Niemeyer, Orthogonal Protein Decoration of DNA Nanostructures, Small, 7, 22, (3211-3218), (2011).
- Barbara Saccà and Christof M. Niemeyer, Covalent Tethering of Protruding Arms for Addressable DNA Nanostructures, Small, 7, 20, (2887-2898), (2011).
- Kasper Jahn, Thomas Tørring, Niels Vinther Voigt, Rasmus Schøler Sørensen, Anne Louise Bank Kodal, Ebbe Sloth Andersen, Kurt Vesterager Gothelf and Jørgen Kjems, Functional Patterning of DNA Origami by Parallel Enzymatic Modification, Bioconjugate Chemistry, 22, 4, (819), (2011).
- Carlos Ernesto Castro, Fabian Kilchherr, Do-Nyun Kim, Enrique Lin Shiao, Tobias Wauer, Philipp Wortmann, Mark Bathe and Hendrik Dietz, A primer to scaffolded DNA origami, Nature Methods, 10.1038/nmeth.1570, 8, 3, (221-229), (2011).
- Na Wu, Xingfei Zhou, Daniel M. Czajkowsky, Ming Ye, Dongdong Zeng, Yanming Fu, Chunhai Fan, Jun Hu and Bin Li, In situ monitoring of single molecule binding reactions with time-lapse atomic force microscopy on functionalized DNA origami, Nanoscale, 3, 6, (2481), (2011).
- Miriam Kaltenbach, Viktor Stein and Florian Hollfelder, SNAP Dendrimers: Multivalent Protein Display on Dendrimer-Like DNA for Directed Evolution, ChemBioChem, 12, 14, (2208), (2011).
- Suchetan Pal, Reji Varghese, Zhengtao Deng, Zhao Zhao, Ashok Kumar, Hao Yan and Yan Liu, Site‐Specific Synthesis and In Situ Immobilization of Fluorescent Silver Nanoclusters on DNA Nanoscaffolds by Use of the Tollens Reaction, Angewandte Chemie, 123, 18, (4262-4265), (2011).
- Franziska Diezmann and Oliver Seitz, DNA-guided display of proteins and protein ligands for the interrogation of biology, Chemical Society Reviews, 10.1039/c1cs15054e, 40, 12, (5789), (2011).
- Michael Erkelenz, Chi-Hsien Kuo and Christof M. Niemeyer, DNA-Mediated Assembly of Cytochrome P450 BM3 Subdomains, Journal of the American Chemical Society, 10.1021/ja204993s, 133, 40, (16111-16118), (2011).
- Thomas Tørring, Niels V. Voigt, Jeanette Nangreave, Hao Yan and Kurt V. Gothelf, DNA origami: a quantum leap for self-assembly of complex structures, Chemical Society Reviews, 40, 12, (5636), (2011).
- Barbara Saccà and Christof M. Niemeyer, Functionalization of DNA nanostructures with proteins, Chemical Society Reviews, 10.1039/c1cs15212b, 40, 12, (5910), (2011).
- Hendrik Eberhard, Franziska Diezmann and Oliver Seitz, DNA as a Molecular Ruler: Interrogation of a Tandem SH2 Domain with Self-Assembled, Bivalent DNA-Peptide Complexes, Angewandte Chemie International Edition, 50, 18, (4146), (2011).
- Zhao Zhao, Erica L. Jacovetty, Yan Liu and Hao Yan, Encapsulation of Gold Nanoparticles in a DNA Origami Cage, Angewandte Chemie, 123, 9, (2089-2092), (2011).
- Suchetan Pal, Reji Varghese, Zhengtao Deng, Zhao Zhao, Ashok Kumar, Hao Yan and Yan Liu, Site‐Specific Synthesis and In Situ Immobilization of Fluorescent Silver Nanoclusters on DNA Nanoscaffolds by Use of the Tollens Reaction, Angewandte Chemie International Edition, 50, 18, (4176-4179), (2011).
- Zhao Zhao, Erica L. Jacovetty, Yan Liu and Hao Yan, Encapsulation of Gold Nanoparticles in a DNA Origami Cage, Angewandte Chemie International Edition, 50, 9, (2041-2044), (2011).
- Mingxu You, Ruo-Wen Wang, Xiaobing Zhang, Yan Chen, Kelong Wang, Lu Peng and Weihong Tan, Photon-Regulated DNA-Enzymatic Nanostructures by Molecular Assembly, ACS Nano, 10.1021/nn204007y, 5, 12, (10090-10095), (2011).
- Hendrik Eberhard, Franziska Diezmann and Oliver Seitz, DNA as a Molecular Ruler: Interrogation of a Tandem SH2 Domain with Self-Assembled, Bivalent DNA-Peptide Complexes, Angewandte Chemie, 123, 18, (4232), (2011).
- Palash K. Dutta, Reji Varghese, Jeanette Nangreave, Su Lin, Hao Yan and Yan Liu, DNA-Directed Artificial Light-Harvesting Antenna, Journal of the American Chemical Society, 10.1021/ja1115138, 133, 31, (11985-11993), (2011).
- Ronnie Pedersen, Jing Kong, Catalina Achim and Thomas LaBean, Comparative Incorporation of PNA into DNA Nanostructures, Molecules, 10.3390/molecules200917645, 20, 9, (17645-17658), (2015).
- Andre V. Pinheiro, Dongran Han, William M. Shih and Hao Yan, Challenges and opportunities for structural DNA nanotechnology, Nature Nanotechnology, 10.1038/nnano.2011.187, 6, 12, (763-772), (2011)., (2011).
- Reza M. Zadegan and Michael L. Norton, Structural DNA Nanotechnology: From Design to Applications, International Journal of Molecular Sciences, 10.3390/ijms13067149, 13, 12, (7149-7162), (2012).
- Andreas Sprengel, Pascal Lill, Pierre Stegemann, Kenny Bravo-Rodriguez, Elisa-C. Schöneweiß, Melisa Merdanovic, Daniel Gudnason, Mikayel Aznauryan, Lisa Gamrad, Stephan Barcikowski, Elsa Sanchez-Garcia, Victoria Birkedal, Christos Gatsogiannis, Michael Ehrmann and Barbara Saccà, Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions, Nature Communications, 10.1038/ncomms14472, 8, (14472), (2017).
